Pressure control device and method for lysine fermentation production

Abstract

The invention discloses a lysine fermentation production pressure control device, which comprises a fermentation tank, the volume of the fermentation tank can be enlarged and reduced by changing the volume of the fermentation tank or the size of a cavity of a container communicated with the fermentation tank; the fermentation tank is provided with a communicated pressure connecting pipe, and the other end of the connecting pipe is provided with a contraction chamber; a spring is arranged in the contraction cavity; and a control push rod is arranged at the other end of the contraction chamber. The defects in the prior art are effectively overcome.

Description

Technical Field

[0001] The invention belongs to the field of bioengineering and equipment technology, and relates to a lysine fermentation production pressure control device and method. Background Art

[0002] L-lysine (referred to as lysine) is a major biotechnology product, mainly used as a food and feed additive, and widely used in food, animal husbandry and breeding industries. At present, it is obtained by adding other chemical agents to organic matter such as soybeans and corn to prepare a culture medium, and then inoculating bacteria for fermentation. During the fermentation process, the nitrogen source, carbon source, pH, pressure, conversion rate, etc. must be strictly controlled, and various solutions or gases must be added to maintain the environment required by the bacteria in the tank.

[0003] At present, the pressure is controlled by the air valve, pressurization is achieved by air intake, and decompression is achieved by air discharge. This method can well control the pressure inside the tank, but it has defects. When the outside gas enters the tank, it will change the gas composition content inside the tank, as well as factors such as temperature; miscellaneous bacteria in the air enter the tank and contaminate it. Summary of the invention

[0004] In order to solve the above problems, the present invention discloses a lysine fermentation production pressure control device and method to overcome the shortcomings of the prior art.

[0005] The present invention is achieved through the following technical solutions: A lysine fermentation production pressure control device comprises a fermentation tank, the volume of the fermentation tank can be expanded and reduced, and the expansion and reduction are achieved by changing the volume of the fermentation tank or the size of the cavity of the container connected to the fermentation tank; The fermentation tank is provided with a communicating pressure connecting pipe, the other end of the connecting pipe is provided with a contraction chamber; a spring is provided in the contraction chamber; and the other end of the contraction chamber is provided with a control push rod.

[0006] The contraction chamber is made of rubber material and adopts a bellows; the spring is elastically fitted with the control push rod.

[0007] The diameter of the connecting pipe is not less than 1 / 3 of the diameter of the fermentation tank.

[0008] An air supply pipe is arranged behind the connecting pipe; the air supply pipe is connected with the pump group and the sterile gas tank through a valve.

[0009] A lysine fermentation production pressure control device comprises a fermentation tank, wherein the fermentation tank is segmented; during the fermentation process, one segment of the fermentation tank can be pressed toward another segment.

[0010] One section of the fermenter may be pressed against another section, which means that the cavity inside the fermenter is squeezed.

[0011] An air bag is arranged in one section of the fermentation tank, and the air bag is connected to an external pump group through a pipeline, and shrinkage is achieved through the pump group.

[0012] The method is that during the fermentation process, the pressure required by the bacteria becomes larger, but the pressure in the fermentation tank is too small, and the contraction chamber is pushed by the control push rod, the contraction chamber is compressed, and the internal gas flows back to the fermentation tank to achieve pressurization; otherwise, the pressure is reduced.

[0013] The present application adjusts the tank pressure by changing the inner cavity of the fermentation tank, thereby effectively solving the problem of bacterial contamination. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 It is a schematic diagram of the structure of this application; Figure 2 This is another structural diagram. DETAILED DESCRIPTION

[0015] In order to enable those skilled in the art to better understand the technical solution in the present application, the technical solution will be fully described below in conjunction with the specific embodiments of the present application. Example

[0016] A lysine fermentation production pressure control device comprises a fermentation tank, wherein the volume of the fermentation tank can be expanded and reduced, and the expansion and reduction are achieved by changing the volume of the fermentation tank or the size of the cavity of a container connected to the fermentation tank.

[0017] according to Figure 1 The fermentation tank 1 is provided with a communicating pressure connecting pipe 2, the other end of which is provided with a contraction chamber 3; a spring 4 is provided in the contraction chamber; and a control push rod 5 is provided at the other end of the contraction chamber.

[0018] The contraction chamber is made of rubber material and adopts a bellows; the spring is elastically fitted with the control push rod.

[0019] The diameter of the connecting pipe is not less than 1 / 3 of the diameter of the fermentation tank.

[0020] An air supply pipe is arranged behind the connecting pipe; the air supply pipe is connected with the pump group and the sterile gas tank through a valve.

[0021] The method is that during the fermentation process, the pressure required by the bacteria becomes larger, but the pressure in the fermentation tank is too small, and the contraction chamber is pushed by the control push rod, the contraction chamber is compressed, and the internal gas flows back to the fermentation tank to achieve pressurization; otherwise, the pressure is reduced. Example

[0022] according to Figure 2 A lysine fermentation production pressure control device includes a fermentation tank, which is a segmented fermentation tank; during the fermentation process, one segment of the fermentation tank can be pressed against another segment.

[0023] One section of the fermenter may be pressed against another section, which means that the cavity inside the fermenter is squeezed.

[0024] An air bag 6 is provided in one section of the fermentation tank, and the air bag is connected to an external pump group through a pipeline, and shrinkage is achieved through the pump group.

[0025] The method is that during the fermentation process, when the pressure required by the bacteria increases and the pressure in the fermentation tank is too low, the air bag is inflated through a pipeline, and the gas at the upper part of the fermentation tank flows to the lower part of the fermentation tank to achieve pressurization; otherwise, the pressure is reduced. Example

[0026] For experimental comparison, the experimental group was Example 1, and the control group pressurized the fermentation tank through an air pump and a pipeline.

[0027] In the control group, the fermentation was carried out for 12 hours, the pressure was 0.95-1.05 kPa, and the bacterial contamination rate of the fermentation liquid was measured to be 0.26%.

[0028] In the experimental group, the fermentation was carried out for 12 hours, the pressure was 0.95-1.05 kPa, and the bacterial contamination rate of the fermentation liquid was measured to be 0.03%.

[0029] To sum up, this application eliminates outside air and reduces the bacteria contamination rate by 0.23%. Example

[0030] The control group pressurized the fermentation tank through an air pump and a pipeline, and the experimental group adopted Example 2.

[0031] For 100 L of fermentation liquid, the control group produced 96.38 L of supernatant liquid; the experimental group produced 96.51 L of supernatant liquid.

[0032] Conclusion: For every 100L of fermentation liquid, the yield was slightly increased by 0.13L, and the appropriate temperature and gas composition improved the efficiency of the strain.

[0033] Although the above describes the specific implementation of the present invention in combination with the embodiments, it is not intended to limit the scope of protection of the present invention. Those skilled in the art should understand that it should be clear to those skilled in the art that these modifications or improvements made without departing from the spirit of the present invention all fall within the scope of protection of the present invention.

Claims

1. A lysine fermentation production pressure control device, comprising a fermentation tank, the volume of which can be expanded and reduced by changing the volume of the fermentation tank or the size of the cavity of a container connected to the fermentation tank; The fermentation tank is provided with a communicating pressure connecting pipe, the other end of the connecting pipe is provided with a contraction chamber; a spring is provided in the contraction chamber; and the other end of the contraction chamber is provided with a control push rod.

2. A lysine fermentation production pressure control device according to claim 1, characterized in that: The contraction chamber is made of rubber material and adopts a bellows; the spring is elastically fitted with the control push rod.

3. A lysine fermentation production pressure control device according to claim 1, characterized in that: The diameter of the connecting pipe is not less than 1 / 3 of the diameter of the fermentation tank.

4. A lysine fermentation production pressure control device according to claim 1, characterized in that: An air supply pipe is arranged behind the connecting pipe; the air supply pipe is connected with the pump group and the sterile gas tank through a valve.

5. A lysine fermentation production pressure control device according to claim 1, characterized in that: The invention comprises a fermentation tank, wherein the fermentation tank is segmented; during the fermentation process, one segment of the fermentation tank can be pressed toward another segment.

6. A lysine fermentation production pressure control device according to claim 5, characterized in that: One section of the fermenter may be pressed against another section, which means that the cavity inside the fermenter is squeezed.

7. A lysine fermentation production pressure control device according to claim 6, characterized in that: An air bag is arranged in one section of the fermentation tank, and the air bag is connected to an external pump group through a pipeline, and shrinkage is achieved through the pump group.

8. The device according to claim 1, wherein the method is as follows: during the fermentation process, when the pressure required by the bacteria increases and the pressure in the fermentation tank is too low, the push rod is controlled to push the contraction chamber, the contraction chamber is compressed, and the internal gas flows back to the fermentation tank to achieve pressurization; otherwise, the pressure is reduced.

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