Traditional Chinese Medicine Composition, Preparation Method and Application Thereof in Anti-Hair Loss, Anti-Dandruff and Itch-Relieving Shampoo

Abstract

The present invention belongs to the technical field of traditional Chinese medicines, and mainly relates to a preparation method of a traditional Chinese medicine composition, in particular to the traditional Chinese medicine composition, the preparation method and its application in an anti-hair loss, anti-dandruff and anti-itching shampoo. The traditional Chinese medicine composition is obtained by fermenting a traditional Chinese medicine mixture; the traditional Chinese medicine mixture comprises 40-60 parts by weight of Polygonum multiflorum Thunb., 15-25 parts by weight of ginseng, 25-35 parts by weight of Poria cocos (Schw.) Wolf, 10-20 parts by weight of Mentha haplocalyx Briq., and 50-60 parts by weight of egg white; the fermentation uses a composite strain fermentation, and the composite strain comprises Lactobacillus acidophilus CICC-6082 and Lactobacillus crispatus CICC-25191. The traditional Chinese medicine composition has the efficacy of preventing hair loss, and at the same time also has the efficacy of removing dandruff and relieving itching, and anti-allergy.

Description

Technical Field

[0001] The present invention belongs to the technical field of traditional Chinese medicine, and mainly relates to a preparation method of a traditional Chinese medicine composition, and particularly relates to a traditional Chinese medicine composition, a preparation method thereof, and an application thereof in an anti - hair - loss, anti - dandruff and antipruritic shampoo. Background Art

[0002] With the improvement of people's health awareness, consumers are increasingly inclined to choose natural and non - irritating hair care and washing products. Traditional Chinese medicine shampoos meet this market demand with their unique natural ingredients and mild hair care and washing effects. Although traditional chemical shampoos meet the needs of cleaning and anti - dandruff to a certain extent, the chemical components therein may cause damage to the scalp and hair quality, such as silicone oil blocking hair follicles and sodium lauryl sulfate inhibiting hair growth. Traditional Chinese medicine shampoos use pure natural traditional Chinese medicine plant ingredients, which are mild and non - irritating, and can reduce damage to the scalp and hair quality. Compared with chemical shampoos, traditional Chinese medicine shampoos are safer and healthier to use. Traditional Chinese medicine components not only have a cleaning effect, but also have multiple effects such as nourishing, repairing, anti - hair - loss, and antipruritic. Traditional Chinese medicine shampoos have broad application prospects. In the future, with the continuous progress of technology and the in - depth research of traditional Chinese medicine, traditional Chinese medicine shampoos will pay more attention to the development of personalization, customization, and high efficiency to meet the needs of different consumers. Summary of the Invention

[0003] In view of the above - mentioned problems, the present invention provides an anti - hair - loss, anti - dandruff and antipruritic traditional Chinese medicine composition for use in the field of shampoos. The traditional Chinese medicine composition of the present invention is obtained by fermenting a traditional Chinese medicine mixture; the traditional Chinese medicine mixture includes 40 - 60 parts by weight of Polygonum multiflorum, 15 - 25 parts by weight of ginseng, 25 - 35 parts by weight of Poria cocos, 10 - 20 parts by weight of Mentha haplocalyx Briq., and 50 - 60 parts by weight of egg white; the fermentation uses a composite strain fermentation, and the composite strain includes Lactobacillus acidophilus CICC - 6082 and Lactobacillus crispatus CICC - 25191. This traditional Chinese medicine composition has the effect of anti - hair - loss, and at the same time also has the effects of anti - dandruff, antipruritic and anti - allergy.

[0004] In the first aspect, the present invention provides a preparation method of a traditional Chinese medicine composition, comprising the following steps:

[0005] S1. Raw material pretreatment: Weigh 40 - 60 g of Polygonum multiflorum, 15 - 25 g of ginseng, 25 - 35 g of Poria cocos, 10 - 20 g of Mentha haplocalyx Briq., and 50 - 60 g of egg white; after cleaning, drying and pulverizing the Polygonum multiflorum, ginseng, Poria cocos, and Mentha haplocalyx Briq., the purpose of cleaning and drying is to remove impurities and moisture;

[0006] S2. Preparation of Bacterial Solution: Lactobacillus acidophilus CICC-6082 and Lactobacillus crispatus CICC-25191 were both purchased from China Center of Industrial Culture Collection. Both were inoculated into MRS agar medium and cultured at 37 °C for 20 - 24 h to activate the strains. Subsequently, they were respectively transferred to shake flasks containing 500 - 1000 mL of MRS liquid medium and fermented for another 8 h. As a result, the concentrations of both bacterial solutions reached 2.0 - 2.2×10 8 cfu / mL. Both bacterial solutions were collected and stored in bacterial solution containers;

[0007] S3. Preparation of Ferment: After mixing the raw materials prepared in step S1, 15 - 20 mL of Lactobacillus acidophilus CICC-6082 bacterial solution and 15 - 20 mL of Lactobacillus crispatus CICC-25191 bacterial solution prepared in step S2 were added, and anaerobic fermentation was carried out at 37 °C for 22 h. After fermentation, 3% H2O2 solution was used to inactivate Lactobacillus acidophilus and Lactobacillus crispatus in the ferment, and the required ferment was obtained; it was rich in various active substances and nutrients produced by the co-fermentation of Chinese herbal medicines with Lactobacillus acidophilus and Lactobacillus crispatus;

[0008] S4. Preparation of Traditional Chinese Medicine Composition: The ferment prepared in step S3 was added with 8 times the amount of water and hot water extracted in a water bath at 95 °C for 1.5 h, extracted 2 times, and the filtrates were combined after filtration and concentrated to a crude drug concentration of 0.5 g / mL to obtain the traditional Chinese medicine composition; among them, the crude drug in the crude drug concentration refers to the total mass of Polygonum multiflorum, ginseng, Poria cocos, mint and egg white.

[0009] Preferably: The components of the MRS agar medium used in step S2 of the present invention are as follows: peptone 10 g, beef extract powder 10 g, yeast extract powder 5 g, glucose 20 g, Tween 1 g, sodium acetate 5 g, ammonium citrate 2 g, dipotassium hydrogen phosphate 2 g, magnesium sulfate heptahydrate 0.2 g, manganese sulfate tetrahydrate 0.05 g, agar 15 g, distilled water 1000 mL, and the pH is adjusted to 6.2 - 6.5; the components of the glucose blood liver agar medium are as follows: peptone 2.5 g, beef extract 1.5 g, glucose blood liver 10 g, agar 18, distilled water 1000 mL, and the pH value is 7.1 ± 0.1.

[0010] Preferably: When the raw materials in step S1 of the present invention are crushed, they need to be crushed into 80 - 100 mesh particles by a crusher.

[0011] Preferably: The volume of the MRS liquid medium in step S2 of the present invention is 700 mL.

[0012] Preferably: The volumes of Lactobacillus acidophilus CICC-6082 bacterial solution and Lactobacillus crispatus CICC-25191 bacterial solution in step S3 of the present invention are both 20 mL.

[0013] Preferably, during the boiling process in step S4 of the present invention, stirring should be carried out regularly to prevent the medicinal materials from sticking to the pot or burning at the bottom.

[0014] In a second aspect, the present invention provides a traditional Chinese medicine composition prepared by the above preparation method, and the traditional Chinese medicine composition has the effects of preventing hair loss, removing dandruff and relieving itching, and anti-allergy.

[0015] In a third aspect, the application of the traditional Chinese medicine composition of the present invention in an anti-hair loss, anti-dandruff and anti-itching shampoo or pharmaceutical product.

[0016] Preferably, the traditional Chinese medicine composition is added as an active ingredient in an anti-hair loss, anti-dandruff and anti-itching shampoo or pharmaceutical product, and the addition amount is 0.5-3%.

[0017] The present invention has the following beneficial effects:

[0018] 1. Under the co-fermentation conditions of Lactobacillus acidophilus CICC-6082 and Lactobacillus crispatus CICC-25191 of the present invention, the synergistic effect of Polygonum multiflorum, Panax ginseng and Poria cocos has the effect of preventing hair loss.

[0019] 2. The synergistic effect of the Poria cocos ferment and the Mentha haplocalyx ferment in the present invention has the effect of removing dandruff and relieving itching.

[0020] 3. The synergistic effect of the Polygonum multiflorum ferment, the Panax ginseng ferment and the egg white ferment in the present invention has the effect of anti-allergy.

[0021] 4. The shampoo prepared from the traditional Chinese medicine composition of the present invention is entirely composed of traditional Chinese medicine ingredients. Compared with some shampoos containing chemical components, the traditional Chinese medicine shampoo is milder and safer and is suitable for people with various skin types.

[0022] 5. The traditional Chinese medicine composition of the present invention is rich in various plant nutrient components, and these components can penetrate into the scalp hair follicles to supplement the nutrients required by the scalp and hair strands and improve the hair quality.

[0023] 6. The shampoo prepared from the traditional Chinese medicine composition of the present invention adopts a pure plant formula, does not add any harmful chemical components, has no pollution to the environment, and is also healthier. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following will briefly introduce the drawings required for use in the embodiments or the description of the prior art. Obviously, the following drawings are only some embodiments of the present invention. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.

[0025] Figure 1 It is the hair growth situation of each group of mice after 28 days of drug administration.

[0026] Figure 2 It is the inhibitory effect of the traditional Chinese medicine compositions of Example 2 group and Comparative Examples 8-9 group on bacteria.

[0027] Figure 3 It is the inhibitory effect of the traditional Chinese medicine compositions of Example 2 group and Comparative Examples 8-9 group on fungi.

[0028] Figure 4 It is the anti-allergic effect of the traditional Chinese medicine compositions of Example 3 group and Comparative Examples 10-12 group.

[0029] The following specific examples illustrate the implementation modes of the present invention. Those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification. The present invention can also be implemented or applied through other different specific implementation modes. Various details in this specification can also be modified or changed based on different viewpoints and applications without departing from the spirit of the present invention. It should be noted that, without conflict, the following examples and the features in the examples can be combined with each other.

[0030] Polygonum multiflorum, ginseng, Poria cocos, and Mentha haplocalyx were all purchased from Anhui Kangweifu Pharmaceutical Co., Ltd.; ginger juice was purchased from Qingzhou Yikang Food Factory.

[0031] Example 1

[0032] This example proves that under the condition of co-fermentation of Lactobacillus acidophilus CICC-6082 and Lactobacillus crispatus CICC-25191, Polygonum multiflorum, ginseng, and Poria cocos have a synergistic effect in preventing hair loss.

[0033] This example provides a traditional Chinese medicine composition, and the specific steps are as follows:

[0034] S1. Raw material pretreatment: Weigh 40 g of Polygonum multiflorum, 15 g of ginseng, 25 g of Poria cocos, 10 g of Mentha haplocalyx, and 50 g of egg white; after cleaning Polygonum multiflorum, ginseng, Poria cocos, and Mentha haplocalyx, dry them and crush them into 100-mesh particles respectively. The purpose of cleaning and drying is to remove impurities and moisture;

[0035] S2. Preparation of bacterial liquid: Both Lactobacillus acidophilus CICC-6082 and Lactobacillus crispatus CICC-25191 were purchased from China Center for Industrial Culture Collection. Both were inoculated on MRS agar medium and cultured at 37 °C for 20 h to activate the strains. Subsequently, they were respectively transferred to 700 mL shake flasks of MRS liquid medium and fermented for another 8 h. As a result, the concentrations of both bacterial liquids reached 2.0×10 8 cfu / mL. Both bacterial liquids were collected and stored in bacterial liquid containers;

[0036] S3. Preparation of the fermented product: After mixing the raw materials prepared in step S1, add 20 mL of the Lactobacillus acidophilus CICC-6082 bacterial solution and 20 mL of the Lactobacillus crispatus CICC-25191 bacterial solution prepared in step S2, and perform anaerobic fermentation at 37 °C for 22 h. After fermentation is completed, inactivate the Lactobacillus acidophilus and Lactobacillus crispatus in the fermented product with a 3% H2O2 solution to obtain the required fermented product, which is rich in various active substances and nutrients produced by the co-fermentation of Chinese herbal medicines with Lactobacillus acidophilus and Lactobacillus crispatus;

[0037] S4. Preparation of the traditional Chinese medicine composition: Take the fermented product prepared in step S3, add 8 times the amount of water, and perform hot water extraction in a water bath at 95 °C for 1.5 h. Extract twice, filter the two extraction solutions, combine the filtrates, and concentrate to a crude drug concentration of 0.5 g / mL to obtain the traditional Chinese medicine composition; among them, the crude drug in the crude drug concentration refers to the total mass of Polygonum multiflorum, Panax ginseng, Poria cocos, Mentha haplocalyx, and egg white.

[0038] Comparative example 1: In step S3, Polygonum multiflorum is not added, and the rest of the steps are the same as in Example 1.

[0039] Comparative example 2: In step S3, Panax ginseng is not added, and the rest of the steps are the same as in Example 1.

[0040] Comparative example 3: In step S3, Poria cocos is not added, and the rest of the steps are the same as in Example 1.

[0041] Comparative example 4: In step S2, Lactobacillus acidophilus CICC-6082 is not added, and the rest of the steps are the same as in Example 1.

[0042] Comparative example 5: In step S2, Lactobacillus crispatus CICC-25191 is not added, and the rest of the steps are the same as in Example 1.

[0043] Comparative example 6: Replace Lactobacillus acidophilus CICC-6082 in step S2 with CICC-6091, and the rest of the steps are the same as in Example 1.

[0044] Comparative example 7: Replace Lactobacillus crispatus CICC-25191 in step S2 with CICC-24879, and the rest of the steps are the same as in Example 1.

[0045] Effect of the traditional Chinese medicine composition on hair loss model mice

[0046] 100 SPF - level male C57BL / 6N mice were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. One - week stable breeding was carried out in a constant - temperature oxygen - supply environment at 25 ± 2 °C before the formal experiment. The whole experimental process strictly followed the national regulations on experimental animal management. After 1 - week breeding, alopecia model mice were established. The specific method was as follows: Paraffin and rosin were mixed and melted in a volume ratio of 1:1, cooled to an appropriate temperature, and then evenly applied to the back of the mice. After the colloid solidified, it was quickly removed to remove the back hair of the mice. The depilation area was 4 cm 2 .

[0047] The 100 mice were randomly divided into 10 groups, with 10 mice in each group. Drugs were administered at 8:00 every morning for 28 consecutive days.

[0048] Model group: After modeling, no drugs were administered, and pure water (10 mL / kg) was applied at 8:00 every morning.

[0049] Positive control group (minoxidil group): After modeling, an external spray was used at 8:00 every morning. The specific method was as follows: The back of the mice was smeared with 5% minoxidil spray (0.12 mL / kg).

[0050] Example group (Example 1 group): After modeling, an external traditional Chinese medicine composition was used at 8:00 every morning. The specific method was as follows: The corresponding 20% v / v (diluted with pure water) traditional Chinese medicine composition (10 mL / kg) was applied to the depilated area on the back of the mice, and the skin was massaged with the fingertips for 5 minutes.

[0051] Control group (Control groups 1 - 7): After modeling, an external traditional Chinese medicine composition was used at 8:00 every morning. The specific method was as follows: The corresponding 20% v / v traditional Chinese medicine composition (10 mL / kg) was applied to the depilated area on the back of the mice, and the skin was massaged with the fingertips for 5 minutes.

[0052] After 28 days, the hair growth on the backs of the mice in each group was observed and photographed. The experimental results are shown in Figure 1 ; After photographing, the mice were sacrificed. The skin of the same surface - area depilated areas on the backs of the mice in the model group, positive control group, Example 1 group, and Control groups 1 - 7 was taken. The hair on the skin epidermis was shaved off with a scalpel and weighed precisely to analyze the hair quality of the mice. The results are shown in Table 1.

[0053] Group Number of mice (pcs) Weight of mouse hair (g) Model group 10 0.1171±0.02 Positive control group 10 0.1459±0.02 Example 1 group 10 0.1438±0.02 Comparative example 1 group 10 0.1299±0.02 Comparative example 2 group 10 0.1295±0.02 Comparative example 3 group 10 0.1297±0.02 Comparative example 4 group 10 0.1293±0.02 Comparative example 5 group 10 0.1302±0.02 Comparative example 6 group 10 0.1312±0.02 Comparative example 7 group 10 0.1315±0.02

[0054] Table 1 is the analysis table of the hair quality of mice after 28 - day drug administration, Figure 1 and the hair growth of mice after 28 - day drug administration. From the results, it can be seen that hair grew on the backs of the mice in each group. In the model group, the hair growth in a small part of the area was slower and the hair weight was lighter.

[0055] Compared with the model group, the hair growth in the control groups (Control Groups 1-7) was relatively faster and the hair weight increased relatively; there was little difference between the positive control group and the example group (Example 1 Group). The hair growth was the fastest and the hair weight was the largest. The hair growth in the hair loss area of the mice was lush and uniform, which confirmed that the synergistic effect of Polygonum multiflorum, Panax ginseng, and Poria cocos has the effect of preventing hair loss under fermentation conditions.

[0056] Example 2

[0057] This example proves that the synergistic effect of the fermented product of Poria cocos and the fermented product of Mentha haplocalyx Briq. has the effect of removing dandruff and relieving itching.

[0058] This example provides a traditional Chinese medicine composition, and the specific steps are as follows:

[0059] S1. Raw material pretreatment: Weigh 50 g of Polygonum multiflorum, 20 g of Panax ginseng, 30 g of Poria cocos, 15 g of Mentha haplocalyx Briq., and 55 g of egg white; after cleaning Polygonum multiflorum, Panax ginseng, Poria cocos, and Mentha haplocalyx Briq. and drying them, they are respectively pulverized into 100-mesh particles. The purpose of cleaning and drying is to remove impurities and moisture.

[0060] S2. Preparation of bacterial liquid: Lactobacillus acidophilus CICC-6082 and Lactobacillus crispatus CICC-25191 are both purchased from the China Center for Industrial Culture Collection. Both are inoculated on MRS agar medium and cultured at 37 °C for 22 h to activate the strains. Subsequently, they are respectively transferred to shake flasks of 700 mL of MRS liquid medium and fermented for another 8 h. As a result, the concentrations of both bacterial liquids reach 2.1×10 8 cfu / mL. Both bacterial liquids are collected and stored in a bacterial liquid container;

[0061] S3. Preparation of fermented product: After mixing the raw materials prepared in step S1, add 20 mL of the bacterial liquid of Lactobacillus acidophilus CICC-6082 and 20 mL of the bacterial liquid of Lactobacillus crispatus CICC-25191 prepared in step S2, and ferment anaerobically at 37 °C for 22 h. After fermentation, 3% H2O2 solution is used to inactivate Lactobacillus acidophilus and Lactobacillus crispatus in the fermented product, and the required fermented product is obtained, which is rich in various active substances and nutrients produced by the co-fermentation of Chinese herbal medicines with Lactobacillus acidophilus and Lactobacillus crispatus.

[0062] S4. Preparation of traditional Chinese medicine composition: Take the fermented product prepared in step S3, add 8 times the amount of water, and hot water extract in a water bath at 95 °C for 1.5 h, extract 2 times. After filtering the two extraction liquids, combine the filtrates and concentrate to a crude drug concentration of 0.5 g / mL to obtain the traditional Chinese medicine composition; among them, the crude drug in the crude drug concentration refers to the total mass of Polygonum multiflorum, Panax ginseng, Poria cocos, Mentha haplocalyx Briq., and egg white.

[0063] Control Group 8: In step S3, Poria cocos is not added, and the rest of the steps are the same as those in Example 2.

[0064] Comparative Example 9: In step S3, mint is not added, and the remaining steps are the same as those in Example 2.

[0065] In vitro antibacterial test

[0066] Refer to Appendix C4 "Test Method for Antibacterial Performance of Dissoluble Antibacterial (Inhibitory) Products" in the National Standard of the People's Republic of China GB15797—2002 "Hygiene Standards for Disposable Sanitary Products" for testing. Take 1.0 mL of the test sample and add it to 0.1 mL of the bacterial suspension (concentration about 10 6 cfu / mL), mix evenly, after 20 minutes, add 9 mL of PBS and mix well, dilute by 2 dilution factors, take 1 mL each and place them in two parallel petri dishes, then add the corresponding culture medium and mix evenly for cultivation. Staphylococcus epidermidis (ATCC-49134, purchased from China Center for Industrial Culture Collection) is placed in nutrient agar medium and cultured at 37 °C for 48 h; Malassezia furfur (KWIK-STIK 2PK, purchased from China Center for Industrial Culture Collection) is placed in tryptic soy agar medium and cultured at 30 °C for 4 d; Staphylococcus aureus (CICC-10145, purchased from China Center for Industrial Culture Collection) is placed in tryptic soy agar medium or nutrient agar medium and cultured at 37 °C for 48 h; Pseudomonas aeruginosa (CICC-21954, purchased from China Center for Industrial Culture Collection) is placed in tryptic soy agar medium and cultured at 37 °C for 48 h; Candida albicans (ATCC-10231, purchased from Haibo Biotechnology Co., Ltd.) is placed in potato dextrose agar medium and cultured at 28 °C for 72 h, and colony counting is performed. The above tests are repeated 3 times, and the average value is taken. At the same time, a blank control is set up, and a positive control group (Tongrentang Maoyanning Shampoo) is established.

[0067] Through the antibacterial test, judge the inhibitory effect of the test sample on Staphylococcus epidermidis, Malassezia furfur, Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans. An antibacterial rate greater than 50% indicates that the product has antibacterial effect, that is, anti-dandruff effect, and the higher the antibacterial rate, the better the effect.

[0068] Antibacterial rate calculation formula: X = (A - B) / A × 100%

[0069] Where: X --- antibacterial rate %

[0070] A --- average recovered colonies of the control sample;

[0071] B --- average recovered colonies of the test sample.

[0072] Figure 2 shows the inhibitory effect of the traditional Chinese medicine compositions in the Example 2 group and the Comparative Example 8-9 groups on bacteria, Figure 3This shows the inhibitory effects of the traditional Chinese medicine compositions in Example 2 group and Comparative Examples 8 - 9 group against fungi. From the results, it can be seen that the inhibitory effects of the Example 2 group against bacteria and fungi are significantly higher than those of the Comparative Example groups and also better than those of the positive control group, indicating that the Example 2 group has the best antibacterial effect. Moreover, it can be seen from the figure that the inhibition rates of Example 2 against microorganisms such as Malassezia furfur and Staphylococcus epidermidis reach over 80%, showing an obvious antibacterial and anti-dandruff effect, which confirms that the synergistic effect of the Poria cocos ferment and Mentha haplocalyx ferment in the Example 2 group has a better effect of relieving itching and removing dandruff.

[0073] Example 3

[0074] This example proves that the synergistic effect of the Polygonum multiflorum ferment, Panax ginseng ferment, and egg white ferment has an anti-allergic effect.

[0075] This example provides a traditional Chinese medicine composition, and the specific steps are as follows:

[0076] S1. Raw material pretreatment: Weigh 60 g of Polygonum multiflorum, 25 g of Panax ginseng, 35 g of Poria cocos, 20 g of Mentha haplocalyx, and 60 g of egg white; after cleaning Polygonum multiflorum, Panax ginseng, Poria cocos, and Mentha haplocalyx, dry them and then crush them into 100 - mesh particles respectively. The purpose of cleaning and drying is to remove impurities and moisture.

[0077] S2. Preparation of bacterial liquid: Lactobacillus acidophilus CICC - 6082 and Lactobacillus crispatus CICC - 25191 are both purchased from the China Center for Industrial Culture Collection. Both are inoculated into MRS agar medium and cultured at 37 °C for 24 h to activate the strains. Subsequently, they are respectively transferred to 700 - mL MRS liquid medium shake flasks and fermented for another 8 h. As a result, the concentrations of both bacterial liquids reach 2.2×10 8 cfu / mL. Both bacterial liquids are collected and stored in a bacterial liquid container.

[0078] S3. Preparation of ferment: After mixing the raw materials prepared in step S1, add 20 mL of the Lactobacillus acidophilus CICC - 6082 bacterial liquid and 20 mL of the Lactobacillus crispatus CICC - 25191 bacterial liquid prepared in step S2, and ferment anaerobically at 37 °C for 22 h. After fermentation is completed, inactivate Lactobacillus acidophilus and Lactobacillus crispatus in the ferment with 3% H2O2 solution, and the required ferment is obtained, which is rich in various active substances and nutrients produced by the co - fermentation of Chinese herbal medicines with Lactobacillus acidophilus and Lactobacillus crispatus.

[0079] S4. Preparation of traditional Chinese medicine composition: Take the ferment prepared in step S3 and add 8 times the amount of water, and perform hot water extraction in a water bath at 95 °C for 1.5 h, extract twice. After filtering the two extraction liquids, combine the filtrates and concentrate them to a crude drug concentration of 0.5 g / mL, and the traditional Chinese medicine composition is obtained; among them, the crude drug in the crude drug concentration refers to the total mass of Polygonum multiflorum, Panax ginseng, Poria cocos, Mentha haplocalyx, and egg white.

[0080] Comparative Example 10: In step S3, polygonum multiflorum is not added, and the remaining steps are the same as those in Example 3.

[0081] Comparative Example 11: In step S3, ginseng is not added, and the remaining steps are the same as those in Example 3.

[0082] Comparative Example 12: In step S3, egg white is not added, and the remaining steps are the same as those in Example 3.

[0083] Determination of anti-allergic efficacy by hyaluronidase in vitro inhibition method

[0084] Preparation of experimental reagents:

[0085] Acetic acid buffer solution (pH = 5.6): Measure 1.155 mL of glacial acetic acid and dilute it to 100 mL. Take 4.8 mL as solution A; weigh 2.72 g of sodium acetate crystal, dissolve it in water and make up the volume to 100 mL. Take 45.2 mL as solution B; mix solution A and solution B and make up the volume to 100 mL with water and mix well. Measure its pH value precisely and adjust it to pH = 5.6 with solution A or solution B.

[0086] Ehrlich reagent; Weigh 0.8 g of p-dimethylaminobenzaldehyde and dissolve it in 15 mL of concentrated hydrochloric acid and 15 mL of absolute ethanol.

[0087] Acetylacetone solution; Take 3.5 mL of acetylacetone and dissolve it in 50 mL of 1.0 mol / L sodium carbonate solution.

[0088] The hyaluronidase solution and sodium hyaluronate solution can be directly prepared into corresponding concentrations with pH 5.6 acetic acid buffer solution. Among them, hyaluronidase is purchased from Shanghai Aladdin Biochemical Technology Co., Ltd., and sodium hyaluronate is purchased from Guangzhou Pengyuan Chemical Co., Ltd.

[0089] In vitro anti-allergic experiment: Mix 0.1 mL of CaCl2 solution with a concentration of 0.25 mmol / L and 0.5 mL of hyaluronidase solution with a concentration of 1250 unit / mL, mix well, and incubate at a constant temperature of 37 °C for 20 min; then add 0.5 mL of sample solution and continue to incubate at a constant temperature for 20 min; then add 0.5 mL of sodium hyaluronate solution with a concentration of 0.5 mg / mL and keep warm for 40 min; then add 0.1 mL of NaOH solution with a concentration of 0.4 mol / L and 0.5 mL of acetylacetone solution, and heat in a boiling water bath for 15 min, then immediately cool with ice water for 5 min; finally add 1.0 mL of Ehrlich reagent and dilute it with 3.0 mL of absolute ethanol, let it stand for 30 min to develop color, measure the absorbance value at a wavelength of 530 nm, and calculate the inhibition rate of the sample on hyaluronidase. The experimental results are known from Figure 4 It can be seen.

[0090] Calculation formula:

[0091] In the formula: A is the ABS value of the control solution (using acetic acid buffer solution instead of the sample solution);

[0092] B is the ABS value of the control blank solution (using acetic acid buffer solution instead of the sample solution and enzyme solution);

[0093] C is the ABS value of the sample solution;

[0094] D is the ABS value of the sample blank solution (using acetic acid buffer solution instead of enzyme solution).

[0095] Figure 4 It is the anti-allergic effect of the traditional Chinese medicine composition in Example 3 group and Comparative Examples 10 - 12 groups. As can be seen from the figure, the anti-allergic effects of the traditional Chinese medicine compositions in Comparative Examples 10 - 12 groups are all less than 1.5%, and they cannot play an anti-allergic role. While the anti-allergic effect of the traditional Chinese medicine composition in Example 3 group is greater than 85%, having a good anti-allergic effect, which confirms that the synergistic effect of polygonum multiflorum ferment, ginseng ferment, and egg white ferment has an anti-allergic effect.

[0096] Example 4

[0097] This example provides a traditional Chinese medicine composition, and the specific steps are as follows:

[0098] S1. Raw material pretreatment: Weigh 50 g of polygonum multiflorum, 20 g of ginseng, 30 g of poria cocos, 15 g of mentha haplocalyx, and 55 g of egg white; after cleaning polygonum multiflorum, ginseng, poria cocos, and mentha haplocalyx, crush and dry them, and then respectively crush them into 100-mesh particles. The purpose of cleaning and drying is to remove impurities and moisture;

[0099] S2. Preparation of bacterial liquid: Lactobacillus acidophilus CICC - 6082 and Lactobacillus crispatus CICC - 25191 are both purchased from the China Center for Industrial Culture Collection. Both are inoculated into MRS agar medium and cultured at 37°C for 22 h to activate the strains. Subsequently, they are respectively transferred to 700 mL of MRS liquid medium shake flasks and fermented for another 8 h. As a result, the concentrations of both bacterial liquids reach 2.1×10 8 cfu / mL. Both bacterial liquids are collected and stored in a bacterial liquid container;

[0100] S3. Preparation of the fermented product: After mixing the raw materials prepared in step S1, add 20 mL of the Lactobacillus acidophilus CICC-6082 bacterial solution and 20 mL of the Lactobacillus crispatus CICC-25191 bacterial solution prepared in step S2, and perform anaerobic fermentation at 37 °C for 22 h. After fermentation is completed, inactivate the Lactobacillus acidophilus and Lactobacillus crispatus in the fermented product with a 3% H2O2 solution to obtain the required fermented product, which is rich in various active substances and nutrients produced by the co-fermentation of Chinese herbal medicines with Lactobacillus acidophilus and Lactobacillus crispatus;

[0101] S4. Preparation of the traditional Chinese medicine composition: Take the fermented product prepared in step S3, add 8 times the amount of water, and perform hot water extraction in a water bath at 95 °C for 1.5 h. Extract twice. After filtering the two extraction solutions, combine the filtrates and concentrate to a crude drug concentration of 0.5 g / mL to obtain the traditional Chinese medicine fermented extract. Then, add ginger juice at 5% v / v of the amount of the traditional Chinese medicine fermented extract, and stir evenly to obtain the traditional Chinese medicine composition; among them, the crude drug in the crude drug concentration refers to the total mass of Polygonum multiflorum, ginseng, Poria cocos, Mentha haplocalyx, and egg white.

[0102] Application Example 1

[0103] Add the traditional Chinese medicine composition obtained in Example 4 as an active ingredient to an anti-hair loss, anti-dandruff, and itching-relieving shampoo product or medicament, and the addition amount of the traditional Chinese medicine composition is 1%.

[0104] The above embodiments only represent the specific implementation manners of the present invention. The description is relatively specific and detailed, but it should not be construed as a limitation on the protection scope of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present invention, any other changes, modifications, substitutions, combinations, and simplifications made without departing from the spirit and principle of the present invention shall be equivalent replacement methods and are all included in the protection scope of the present invention.

Claims

1. An anti-hair loss, anti-dandruff and itch-relieving traditional Chinese medicine composition, characterized in that: The traditional Chinese medicine composition is obtained by fermenting a traditional Chinese medicine mixture; The traditional Chinese medicine mixture is composed of the following raw materials in parts by weight: 40 - 60 parts of Polygonum multiflorum, 15 - 25 parts of ginseng, 25 - 35 parts of Poria cocos, 10 - 20 parts of Mentha haplocalyx Briq., and 50 - 60 parts of egg white; The composite strain used for the fermentation consists of Lactobacillus acidophilus CICC - 6082 and Lactobacillus crispatus CICC - 25191.

2. The anti - hair - loss, anti - dandruff and itch - relieving traditional Chinese medicine composition according to claim 1, wherein: This traditional Chinese medicine composition has the effects of preventing hair loss, removing dandruff and relieving itching, and anti - allergy.

3. The preparation method of the anti - hair - loss, anti - dandruff and antipruritic traditional Chinese medicine composition according to claim 1, characterized in that: The specific steps are as follows: S1. Raw material pretreatment: Weigh the following raw materials, 40 - 60 g of Polygonum multiflorum, 15 - 25 g of ginseng, 25 - 35 g of Poria cocos, 10 - 20 g of Mentha haplocalyx Briq., and 50 - 60 g of egg white; after cleaning the Polygonum multiflorum, ginseng, Poria cocos, and Mentha haplocalyx Briq., dry and crush them; S2. Preparation of bacterial liquid: Select Lactobacillus acidophilus CICC-6082 and Lactobacillus crispatus CICC-25191, inoculate them into MRS agar medium respectively, culture at 37 °C for 20 - 24 h to obtain activated strains, transfer these two activated strains into shake flasks containing 500 - 1000 mL of MRS liquid medium respectively, continue fermentation for 8 h, and when the concentrations of both bacterial liquids reach 2.0 - 2.2×10 8 cfu / mL, collect and store them in special bacterial liquid containers respectively; S3. Fermentation: Mix the raw materials prepared in step S1 evenly, then add 15 - 20 mL of the Lactobacillus acidophilus CICC - 6082 bacterial liquid and 15 - 20 mL of the Lactobacillus crispatus CICC - 25191 bacterial liquid prepared in step S2, and carry out anaerobic fermentation at 37 °C for 22 h. After the fermentation is completed, use 3% H2O2 solution to inactivate the Lactobacillus acidophilus and Lactobacillus crispatus in the fermented product, so as to obtain the required fermented product; S4. Preparation of traditional Chinese medicine composition: Take the fermented product prepared in step S3, add 8 times of water, carry out hot water extraction in a water bath at 95 °C for 1.5 h, extract 2 times, filter, combine the filtrates and concentrate to a crude drug concentration of 0.5 g / mL to obtain the traditional Chinese medicine composition; among them, the crude drug in the crude drug concentration refers to the total mass of Polygonum multiflorum, ginseng, Poria cocos, Mentha haplocalyx Briq., and egg white.

4. The preparation method of the anti - hair - loss, anti - dandruff and itch - relieving traditional Chinese medicine composition according to claim 3, characterized in that: The components of the MRS agar medium used in step S2 are: 10 g of peptone, 10 g of beef extract powder, 5 g of yeast extract powder, 20 g of glucose, 1 g of Tween, 5 g of sodium acetate, 2 g of ammonium citrate, 2 g of dipotassium hydrogen phosphate, 0.2 g of magnesium sulfate heptahydrate, 0.05 g of manganese sulfate monohydrate, 15 g of agar, and 1000 mL of distilled water.

5. The preparation method of the anti-hair loss, anti-dandruff and itch-relieving traditional Chinese medicine composition according to claim 3, characterized in that: When crushing the raw materials in step S1, it is necessary to crush them into particles of 80 - 100 mesh with a crusher.

6. The preparation method of the anti - hair - loss, anti - dandruff and antipruritic traditional Chinese medicine composition according to claim 3, characterized in that: The volume of the Lactobacillus acidophilus CICC - 6082 bacterial liquid in step S3 is 20 mL.

7. The preparation method of the anti - hair - loss, anti - dandruff and itch - relieving traditional Chinese medicine composition according to claim 3, wherein: The volume of the Lactobacillus crispatus CICC - 25191 bacterial liquid in step S3 is 20 mL.

8. Use of the anti - hair - loss and anti - dandruff and itching traditional Chinese medicine composition according to claim 1 or 2 in the preparation of anti - hair - loss and anti - dandruff and itching shampoo.

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