A Lactobacillus plantarum hypoglycemic preparation and preparation method thereof

Abstract

The invention relates to the technical field of bacterial agents, and in particular to a lactobacillus plantarum hypoglycemic preparation and a preparation method thereof. Lactobacillus plantarum, lactobacillus acidophilus, lactobacillus casei, lactobacillus rhamnosus and streptococcus thermophilus are uniformly mixed, subjected to two anaerobic cultures, and then dried, anaerobic granulated and anaerobic coated to obtain the lactobacillus plantarum hypoglycemic preparation. The invention realizes real-time monitoring of the bacterial culture and evaluation and judgment of the risk of the bacterial culture by analyzing the change of bacterial concentration during the bacterial culture process, so as to facilitate the subsequent timely repair, maintain the bacterial culture under a normal environment, ensure the high quality of the bacterial culture, accurately regulate the bacterial cells in a culture box by analyzing the time lag between an abnormal oxygen content value and a warning of the culture bacterial concentration, ensure the high quality of the bacterial culture, and make the finally prepared lactobacillus plantarum hypoglycemic preparation higher in quality.

Description

Technical Field

[0001] The invention relates to the technical field of bacterial agents, and in particular to a Lactobacillus plantarum hypoglycemic preparation and a preparation method thereof. Background Art

[0002] Lactobacillus plantarum is a type of lactic acid bacteria. As one of the normal intestinal flora, Lactobacillus plantarum can maintain the stability of the pH of the intestinal environment, adjust the microbial flora in the gastrointestinal tract, and improve symptoms such as diarrhea, constipation, and indigestion. In addition, the various antibacterial substances such as organic acids and bacteriocins produced by Lactobacillus plantarum can promote the absorption of nutrients and enhance the body's immunity.

[0003] Chinese patent publication number CN112940984A relates to a composite lactobacillus preparation for resisting Helicobacter pylori, lowering blood sugar, regulating the stomach and increasing immunity, and its preparation method and application. It adopts plant lactobacillus MiuCul-01, acidophilus lactobacillus MiuLac-01, casei lactobacillus MiaoTech-01, rhamnosus lactobacillus MiaoTech-11, and thermophilic streptococcus MiaoPro-01, and is prepared by a new process combining mixed bacteria inoculation, liquid culture, solid-liquid separation, and solid-state culture, so as to obtain a composite lactobacillus cell with higher vitality, and also retains antibacterial substances such as bacteriocins and organic acids produced during the solid-state culture process, and has the function of resisting Helicobacter pylori and regulating the stomach; it can also produce a variety of metabolites with the functions of lowering blood sugar and increasing immunity. The solid-state culture has a low moisture content and is easy to dry, which can reduce the stress death of the composite lactobacillus during the drying period. Combined with appropriate granulation and coating processes, it can greatly improve the vitality of the composite lactobacillus in the product. The compound lactobacillus probiotic preparation produced can enhance the effects of fighting Helicobacter pylori, lowering blood sugar, regulating the gastrointestinal tract and increasing immunity.

[0004] In the prior art, when Lactobacillus plantarum is used to prepare hypoglycemic preparations, the most influential factor in the preparation process is bacterial culture, and the quality of the culture directly affects the quality of the Lactobacillus plantarum hypoglycemic preparations. At present, in the preparation process of Lactobacillus plantarum hypoglycemic preparations, the parameters of the culture process are usually pre-set to complete the cultivation of Lactobacillus plantarum, and this preparation method cannot realize real-time monitoring and analysis of the culture process. Furthermore, it is also impossible to analyze the oxygen environment, one of the important parameters of the culture, to determine whether it causes interference to the current culture quality. Summary of the invention

[0005] The object of the present invention is to provide a Lactobacillus plantarum hypoglycemic preparation and a preparation method thereof. The technical problem solved by the present invention is that it is impossible to realize real-time monitoring and analysis of the culture process, and further, it is impossible to analyze the oxygen environment, which is one of the important parameters of the culture, to determine whether it causes interference to the current culture quality.

[0006] The purpose of the present invention can be achieved by the following technical solutions:

[0007] A method for preparing a Lactobacillus plantarum hypoglycemic preparation comprises the following steps:

[0008] Lactobacillus plantarum MiuCul-01 (Lactobacillus plantarum); Lactobacillus acidophilus MiuLac-01 (Lactobacillus acidophilus), Lactobacillus casei MiaoTech-01 (Lactobacillus casei), Lactobacillus rhamnosus MiaoTech-11 (Lactobacillus rhamnosus), Streptococcus thermophilus MiaoPro-01 (Streptococcusthermophilus) are mixed uniformly, and subjected to two anaerobic cultures, followed by drying, anaerobic granulation, and anaerobic coating to obtain a Lactobacillus plantarum hypoglycemic preparation;

[0009] During the two anaerobic cultures, a culture monitoring process is provided, which includes the following steps:

[0010] S121: during the bacterial cell culture process, collection time points at the same time interval are set, and the bacterial cells in the incubator are sampled for quality inspection at each collection time point to obtain samples to be tested, bacterial cell data of the samples to be tested are obtained by plate counting method, and data analysis is performed on the bacterial cell data to obtain a culture monitoring value;

[0011] S122: Acquire the culture monitoring value during the bacterial culture process, and determine whether the culture condition of the bacterial body is risky based on the culture monitoring value. If the culture condition shows a high risk, generate a culture repair signal;

[0012] S123: based on the generated culture repair signal, obtaining suspected impact data causing a high risk of bacterial culture, wherein the suspected impact data includes an oxygen content value of the incubator, and performing data processing on the suspected impact data to obtain a similarity overlap ratio;

[0013] S124: judging whether the obtained cultured bacterial concentration warning is caused by abnormal oxygen content based on the similarity overlap ratio, and if it is caused by abnormal dissolved oxygen concentration, generating an impact signal;

[0014] S125: Based on the impact signal, a control instruction is sent to control the ventilation frequency of the incubator.

[0015] As a further solution of the present invention: the specific method of obtaining the culture monitoring value is:

[0016] Obtain the concentration value of the cultured bacteria in the sample to be tested, and compare it with the concentration warning value and the concentration standard value;

[0017] If the concentration value of the cultured bacteria is lower than the standard concentration value, a bacteria abnormality signal is generated;

[0018] If the concentration value of the cultured bacteria is greater than or equal to the concentration standard value, and the concentration value of the cultured bacteria is less than the concentration warning value, a bacteria warning signal is generated;

[0019] If the concentration value of the cultured bacteria is greater than or equal to the concentration warning value, a normal bacteria signal is generated;

[0020] When there are no abnormal bacterial signals and there are bacterial warning signals or normal bacterial signals, culture risk monitoring and analysis is performed;

[0021] The process of developing risk monitoring analysis is as follows:

[0022] By formula The culture monitoring value KN is obtained, where s1 and s2 are preset proportional coefficients, KZ is expressed as the abnormal number ratio, and NZ is expressed as the abnormal degree ratio.

[0023] As a further solution of the present invention: the process of obtaining the abnormal number ratio KZ is:

[0024] The number of all samples that generate bacterial warning signals among the samples to be tested is obtained, and the ratio is processed with the total number of samples to be tested to obtain the abnormal number ratio, which is marked as KZ.

[0025] As a further solution of the present invention: the process of obtaining the abnormality degree ratio NZ is:

[0026] Obtain the bacterial concentration value of the sample that generates a bacterial warning signal, perform subtraction processing on it and the concentration warning value and take the absolute value to obtain the concentration warning difference, sum and average the concentration warning differences of all samples that generate bacterial warning signals to obtain the concentration warning mean, and perform ratio processing on it and the concentration warning value to obtain the abnormality ratio, which is marked as NZ.

[0027] As a further solution of the present invention: the process of generating the culture repair signal is:

[0028] If the culture monitoring value is greater than the culture monitoring threshold, a culture repair signal is generated.

[0029] As a further solution of the present invention: the process of obtaining the similarity coincidence ratio is:

[0030] Obtain the incubator oxygen content value at the acquisition time point and compare it with the oxygen content threshold value;

[0031] If the oxygen content value is less than the oxygen content threshold, a normal oxygen content signal is generated;

[0032] If the oxygen content value is greater than or equal to the oxygen content threshold, an oxygen content abnormality signal is generated;

[0033] The abnormal oxygen content signal or the normal oxygen content signal generated at the same collection point time is compared and analyzed with the normal bacterial cell signal or the bacterial cell warning signal, respectively. If the abnormal oxygen content signal and the bacterial cell warning signal, or the normal oxygen content signal and the bacterial cell normal signal are generated at the same time, the collection point time is marked as a coincident collection point;

[0034] The number of overlapping acquisition points is obtained, and the number of overlapping acquisition points is ratioed to the number of all acquisition time points to obtain a similarity overlap ratio.

[0035] As a further solution of the present invention: the generation process of the influence signal is:

[0036] If the similarity overlap ratio is greater than or equal to the similarity overlap ratio threshold, an influence signal is generated.

[0037] As a further solution of the present invention: the process of regulating the ventilation frequency of the incubator is:

[0038] Extract the bacterial early warning signal collection time interval corresponding to the maximum concentration value of the cultured bacteria, obtain the start time and end time of the bacterial early warning signal collection time interval, and then use the start time as the base point to collect oxygen content values ​​at each time point in sequence, determine the time point when the oxygen content normal signal turns into the oxygen content abnormal signal, and record it as the reference time point;

[0039] By formula , the ventilation frequency control value TH is calculated; wherein, PY is the preset ventilation frequency of the bacteria during the culture process, TS represents the starting time of the bacteria warning signal collection time interval, TJ represents the end time of the bacteria warning signal collection time interval, and TC represents the reference time point.

[0040] As a further solution of the present invention: the process of obtaining the bacterial early warning signal collection time interval is:

[0041] When the impact signal is obtained, all adjacent bacterial early warning signals are combined and processed to obtain the bacterial early warning signal collection time interval.

[0042] A Lactobacillus plantarum hypoglycemic preparation comprises the following components: 10-15 parts of Lactobacillus plantarum MiuCul-01; 5-10 parts of Lactobacillus acidophilus MiuLac-01; 3-7 parts of Lactobacillus casei MiaoTech-01; 2-6 parts of Lactobacillus rhamnosus MiaoTech-11; and 8-12 parts of Streptococcus thermophilus MiaoPro-01.

[0043] Beneficial effects of the present invention:

[0044] The plant lactobacillus hypoglycemic preparation of the present invention is prepared by mixing plant lactobacillus with other bifidobacteria, and through the synergistic effect of these lactobacilli and bifidobacteria, the obtained probiotic preparation product has the effects of lowering blood sugar, increasing immunity, etc.

[0045] The present invention sets collection time points at the same time interval during the bacterial cell culture process, performs sampling quality inspection on the bacterial cells in the culture box at each collection time point to obtain samples to be tested, obtains bacterial cell data of the samples to be tested by a plate counting method, performs data analysis on the bacterial cell data, and obtains a culture monitoring value; obtains the culture monitoring value during the bacterial cell culture process, and judges whether the culture condition of the bacterial cells is risky based on the culture monitoring value; if the culture condition shows a relatively high risk, generates a culture repair signal; the present invention realizes real-time monitoring of bacterial cell culture and evaluates and judges the risk of bacterial cell culture by analyzing the change of bacterial cell concentration during the bacterial cell culture process, so as to facilitate the subsequent timely repair thereof, maintain the bacterial cell culture under a normal environment, ensure the high quality of the bacterial cell culture, and make the finally prepared Lactobacillus plantarum hypoglycemic preparation higher in quality;

[0046] The present invention obtains suspected influencing data causing a high risk of bacterial culture based on the generated culture repair signal, wherein the suspected influencing data includes the oxygen content value of the culture box, performs data processing on the suspected influencing data to obtain a similarity overlap ratio; based on the similarity overlap ratio, it is judged whether the obtained culture bacterial concentration warning is caused by abnormal oxygen content, and if it is caused by abnormal dissolved oxygen concentration, a control signal is generated; based on the influencing signal, a control instruction is sent to control the ventilation frequency of the culture box; the present invention analyzes the correlation influence of the oxygen content value and the culture bacterial concentration warning, and determines that under its influence, there is a phenomenon of incompatibility between the current bacteria and the preset ventilation frequency, based on this, by analyzing the time lag between the abnormal oxygen content value and the culture bacterial concentration warning, the bacteria in the culture box are accurately regulated to ensure the high quality of the bacterial culture, so that the final Lactobacillus plantarum hypoglycemic preparation is of higher quality. BRIEF DESCRIPTION OF THE DRAWINGS

[0047] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following briefly introduces the drawings required for use in the description of the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0048] Figure 1 The present invention provides a flowchart of a method for preparing a Lactobacillus plantarum hypoglycemic preparation provided in Example 1. DETAILED DESCRIPTION

[0049] In order to enable those skilled in the art to better understand the scheme of the present invention, the technical scheme in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work should fall within the scope of protection of the present invention.

[0050] Embodiment 1: Figure 1 As shown, a Lactobacillus plantarum hypoglycemic preparation and a preparation method provided by an embodiment of the present invention specifically include the following steps:

[0051] S1. Lactobacillus plantarum MiuCul-01 (Lactobacillus plantarum), with a preservation number of CGMCC20865, purchased from Shanxi Haocheng Biotechnology Co., Ltd.; Lactobacillus acidophilus MiuLac-01 (Lactobacillus acidophilus), with a preservation number of CGMCC 20864, ​​purchased from Shandong Jiacheng Biotechnology Co., Ltd.; Lactobacillus casei MiaoTech-01 (Lactobacillus casei), with a preservation number of CGMCC 20863, purchased from Xi'an Mixianer Biotechnology Co., Ltd.; Lactobacillus rhamnosus MiaoTech-11 (Lactobacillus rhamnosus), purchased from Xi'an Qianyecao Biotechnology Co., Ltd., with a preservation number of CGMCC 20862; Streptococcus thermophilus MiaoPro-01 (Streptococcus thermophilus), with a preservation number of CGMCC 20861, purchased from Native Peptide Biotechnology (Tianjin) Co., Ltd., mixed evenly, and placed in a liquid incubator for the first anaerobic culture. The resulting culture fluid was centrifuged to obtain mixed cells of Lactobacillus plantarum for later use;

[0052] S2, to the mixed thalline of obtained plant lactobacillus and solid culture box, carry out second anaerobic culture under sealed state, carry out low temperature drying or freeze drying or vacuum drying after culturing to form the mixed thalline solid of plant lactobacillus;

[0053] S3, crushing the mixed bacterial solids of Lactobacillus plantarum, anaerobically granulating, and anaerobically coating to obtain a Lactobacillus plantarum hypoglycemic preparation;

[0054] In the preparation method of the above-mentioned Lactobacillus plantarum hypoglycemic preparation, the control of anaerobic culture conditions will have a great influence on the growth rate and metabolic activity of the obtained bacteria in the two cultures of the mixed bacteria composed of Lactobacillus plantarum and the like, and will further affect the quality of the preparation of the Lactobacillus plantarum hypoglycemic preparation. Therefore, a culture monitoring process is provided in the first anaerobic culture and the second anaerobic culture, and the culture monitoring process includes the following steps:

[0055] S121: during the bacterial cell culture process, collection time points at the same time interval are set, and the bacterial cells in the incubator are sampled for quality inspection at each collection time point to obtain samples to be tested, bacterial cell data of the samples to be tested are obtained by plate counting method, and data analysis is performed on the bacterial cell data to obtain a culture monitoring value;

[0056] In some embodiments, during the culture process of the mixed bacteria composed of Lactobacillus plantarum and the like, the preset culture time is divided into a plurality of collection time points at the same time interval, and at each collection time point, a sample of the bacteria in the incubator is selected to obtain a sample to be tested, and the concentration value of the cultured bacteria of the sample to be tested is obtained by the plate count method;

[0057] Then, the concentration value of the cultured bacteria is analyzed to determine the culture monitoring value;

[0058] Specifically, the specific method for obtaining the culture monitoring value is:

[0059] Obtain the concentration value of the cultured bacteria in the sample to be tested, and compare it with the concentration warning value and the concentration standard value; it should be explained that the concentration warning value is pre-set by the technicians, which is greater than the concentration standard value, indicating the safe setting range of the bacteria concentration, so that when the bacteria concentration decreases, the bacteria culture environment can be controlled in time;

[0060] The meaning of the concentration standard value is that if it is less than the concentration standard value, it means that the bacterial culture effect is not good, and if it is greater than the concentration standard value, it means that the bacterial culture effect meets the process requirements;

[0061] If the concentration value of the cultured bacteria is less than the standard concentration value, it means that the concentration of the bacteria detected at the collection time point does not meet the standard of bacterial culture, and a bacterial abnormality signal is generated;

[0062] If the concentration value of the cultured bacteria is less than the concentration warning value and greater than or equal to the concentration standard value, it means that the bacteria concentration detected at the collection time point exceeds the concentration warning range preset in the process, and a bacteria warning signal is generated;

[0063] If the concentration value of the cultured bacteria is greater than or equal to the concentration warning value, it means that the concentration of the bacteria detected at the collection time point does not exceed the concentration warning range preset in the process, and a normal bacteria signal is generated;

[0064] First, the number of samples with abnormal bacterial signals, bacterial warning signals, and normal bacterial signals in the samples to be tested is obtained. If an abnormal bacterial signal appears within the sample collection time, the bacterial culture equipment and environment are immediately checked at the collection point. If no abnormal bacterial signal appears and a bacterial warning signal or a normal bacterial signal appears, the culture risk monitoring and analysis is performed. If all normal bacterial signals appear, the bacterial culture work is continued;

[0065] The process of developing risk monitoring analysis is as follows:

[0066] Obtain the number of samples that generate bacterial warning signals among the samples to be tested, and perform ratio processing on it with the total number of samples to be tested to obtain the abnormal number ratio, which is marked as KZ;

[0067] Obtain the bacterial concentration value of the sample generating bacterial warning signal, perform subtraction processing on it and the concentration warning value and take the absolute value to obtain the concentration warning difference, sum and average the concentration warning differences of all samples generating bacterial warning signals to obtain the concentration warning mean, and perform ratio processing on it and the concentration warning value to obtain the abnormality ratio, which is marked as NZ;

[0068] The obtained abnormal number ratio KZ and abnormal degree ratio NZ are processed and the formula is used The culture monitoring value KN is obtained, where s1 and s2 are both preset proportional coefficients, s1=2.314, s2=2.921;

[0069] It should be noted that the purpose of the culture monitoring value KN is to analyze the number of samples with cultured bacterial concentration warnings and the degree of sample warnings in the samples to be tested, and then judge the culture status of the bacteria;

[0070] S122: Acquire the culture monitoring value during the bacterial culture process, and determine whether the culture condition of the bacterial body is risky based on the culture monitoring value. If the culture condition shows a high risk, generate a culture repair signal;

[0071] In some embodiments, a culture monitoring value during bacterial culture is obtained and compared with a culture monitoring threshold value;

[0072] If the culture monitoring value is less than or equal to the culture monitoring threshold, it means that during the culture process, the number of warnings and the degree of warning values ​​of the bacterial concentration are small, and the culture collection can be continued to complete the bacterial culture work;

[0073] If the culture monitoring value is greater than the culture monitoring threshold, it means that the culture risk of the cultured bacteria in the pond to be repaired is low, and a culture repair signal is generated;

[0074] The technical scheme of the above steps is: in the process of bacterial cell cultivation, collection time points with the same time interval are set, and the bacteria in the culture box are sampled for quality inspection at each collection time point to obtain samples to be inspected, and the bacterial cell data of the samples to be inspected are obtained by a plate counting method, and the bacterial cell data are analyzed to obtain a cultivation monitoring value; the cultivation monitoring value in the process of bacterial cell cultivation is obtained, and whether the cultivation situation of the bacteria is risky is judged based on the cultivation monitoring value, and if the cultivation situation shows a greater risk, a cultivation repair signal is generated. The present invention realizes real-time monitoring of bacterial cell cultivation and evaluates and judges the risk of bacterial cell cultivation by analyzing the change of bacterial cell concentration in the process of bacterial cell cultivation, so as to facilitate the subsequent timely repair, maintain the bacterial cell cultivation under a normal environment, ensure the high quality of bacterial cell cultivation, and make the final prepared plant lactobacillus hypoglycemic preparation higher in quality.

[0075] S123: based on the generated culture repair signal, obtaining suspected impact data causing a high risk of bacterial culture, wherein the suspected impact data includes an oxygen content value of the incubator, and performing data processing on the suspected impact data to obtain a similarity overlap ratio;

[0076] Among them, a fluorescence sensor is arranged in the incubator to collect the oxygen content in the incubator; the fluorescence sensor is based on the interaction between oxygen molecules and fluorescent materials, and measures the oxygen concentration through the fluorescence quenching effect;

[0077] In some embodiments, the oxygen content value of the incubator at the collection time point is obtained and compared with the oxygen content threshold value;

[0078] If the oxygen content value is less than the oxygen content threshold, a normal oxygen content signal is generated;

[0079] If the oxygen content value is greater than or equal to the oxygen content threshold, an oxygen content abnormality signal is generated;

[0080] The abnormal oxygen content signal or normal oxygen content signal generated at the same collection point time is compared and analyzed with the normal bacterial cell signal or bacterial cell warning signal respectively. If the abnormal oxygen content signal and bacterial cell warning signal, or the normal oxygen content signal and bacterial cell normal signal are generated at the same time, the collection point time is marked as a coincident collection point, otherwise, it is recorded as a deviated collection point;

[0081] Obtain the number of overlapping acquisition points, and perform ratio processing on the number of overlapping acquisition points and the number of all acquisition time points to obtain a similarity overlap ratio;

[0082] It should be noted that the purpose of the similarity coincidence ratio is to analyze the coincidence between the oxygen content value of the incubator and the cultured bacterial concentration, and to determine whether the cultured bacterial concentration warning is caused by abnormal oxygen content, so as to facilitate the subsequent regulation to promote bacterial cultivation and achieve a more efficient repair of the bacterial culture environment;

[0083] S124: judging whether the obtained cultured bacterial concentration warning is caused by abnormal oxygen content based on the similarity overlap ratio, and if it is caused by abnormal dissolved oxygen concentration, generating an impact signal;

[0084] Specifically, the obtained similarity overlap ratio is compared with a similarity overlap ratio threshold;

[0085] If the similarity overlap ratio is greater than or equal to the similarity overlap ratio threshold, it means that the culture bacterial concentration warning is caused by abnormal oxygen content, and an impact signal is generated;

[0086] If the similarity coincidence ratio is less than the similarity coincidence ratio threshold, it means that the culture bacterial concentration warning is not caused by abnormal oxygen content, and the equipment and environment for bacterial culture need to be checked;

[0087] S125: sending a control instruction based on the impact signal to control the ventilation frequency of the incubator;

[0088] Among them, during the bacterial culture process, the purpose of ventilation is to continuously control the atmosphere in the incubator to ensure that the anaerobic environment required by the bacteria meets the culture standards. The current ventilation method uses an interval frequency to input protective gases such as nitrogen into the incubator to ensure the oxygen concentration of the incubator. Since the gas environment of the bacteria will change during the bacterial culture, this fixed frequency ventilation will affect the bacterial culture for a period of time;

[0089] In some embodiments, when an impact signal is obtained, a control instruction is sent to the ventilation equipment (including a ventilation pump) of the incubator. When the control instruction is obtained, all adjacent bacterial warning signals are merged (for example, the 6th, 7th, and 8th collection time points are all bacterial warning signals, which are regarded as the overall bacterial warning signal) to obtain the bacterial warning signal collection time interval;

[0090] Extract the bacterial early warning signal collection time interval corresponding to the maximum concentration value of the cultured bacteria, obtain the starting time of the bacterial early warning signal collection time interval, and then collect oxygen content values ​​in sequence from the starting time forward, and determine the time point when the oxygen content normal signal turns into the oxygen content abnormal signal according to the above-mentioned oxygen content value and oxygen content threshold judgment method, and record it as the reference time point;

[0091] By formula , calculate the ventilation frequency control value TH; where PY is the preset ventilation frequency of the bacteria during the culture process, TS represents the start time of the bacteria warning signal collection time interval, TJ represents the end time of the bacteria warning signal collection time interval, and TC represents the reference time point;

[0092] The obtained ventilation frequency control value TH is sent to the ventilation device of the incubator in the form of a regulation instruction;

[0093] The technical solution of the above steps is as follows: based on the generated culture repair signal, suspected influencing data causing a high risk of bacterial culture is obtained, wherein the suspected influencing data includes the oxygen content value of the incubator, and the suspected influencing data is processed to obtain a similarity overlap ratio; based on the similarity overlap ratio, it is judged whether the obtained culture bacterial concentration warning is caused by abnormal oxygen content, and if it is caused by abnormal dissolved oxygen concentration, a control signal is generated; based on the influencing signal, a control instruction is sent to regulate the ventilation frequency of the incubator; the present invention analyzes the correlation influence of the oxygen content value and the culture bacterial concentration warning, and determines that under its influence, there is a phenomenon of incompatibility between the current bacteria and the preset ventilation frequency. Based on this, by analyzing the time lag between the abnormal oxygen content value and the culture bacterial concentration warning, the bacteria in the incubator are accurately regulated to ensure the high quality of the bacterial culture, so that the final Lactobacillus plantarum hypoglycemic preparation is of higher quality.

[0094] Embodiment 2: A Lactobacillus plantarum hypoglycemic preparation comprises the following components: 10 parts of Lactobacillus plantarum MiuCul-01 (Lactobacillus plantarum); 5 parts of Lactobacillus acidophilus MiuLac-01 (Lactobacillus acidophilus); 3 parts of Lactobacillus casei MiaoTech-01 (Lactobacillus casei); 2 parts of Lactobacillus rhamnosus MiaoTech-11 (Lactobacillus rhamnosus); 8 parts of Streptococcus thermophilus MiaoPro-01 (Streptococcus thermophilus).

[0095] Embodiment 3: A Lactobacillus plantarum hypoglycemic preparation comprises the following components: 12 parts of Lactobacillus plantarum MiuCul-01 (Lactobacillus plantarum); 7 parts of Lactobacillus acidophilus MiuLac-01 (Lactobacillus acidophilus); 5 parts of Lactobacillus casei MiaoTech-01 (Lactobacillus casei); 4 parts of Lactobacillus rhamnosus MiaoTech-11 (Lactobacillus rhamnosus); 10 parts of Streptococcus thermophilus MiaoPro-01 (Streptococcus thermophilus).

[0096] Embodiment 4: A Lactobacillus plantarum hypoglycemic preparation comprises the following components: 10 parts of Lactobacillus plantarum MiuCul-01 (Lactobacillus plantarum); 5 parts of Lactobacillus acidophilus MiuLac-01 (Lactobacillus acidophilus); 3 parts of Lactobacillus casei MiaoTech-01 (Lactobacillus casei); 2 parts of Lactobacillus rhamnosus MiaoTech-11 (Lactobacillus rhamnosus); 8 parts of Streptococcus thermophilus MiaoPro-01 (Streptococcus thermophilus).

[0097] The above formulas are all dimensionless and numerical calculations. The formula is a formula for the most recent real situation obtained by collecting a large amount of data and performing software simulation. The preset parameters in the formula are set by technicians in this field according to actual conditions.

[0098] The above is a detailed description of an embodiment of the present invention, but the content is only a preferred embodiment of the present invention and cannot be considered to limit the scope of implementation of the present invention. All equivalent changes and improvements made within the scope of the present invention should still fall within the scope of the patent coverage of the present invention.

Claims

1. A method for preparing a Lactobacillus plantarum hypoglycemic preparation, characterized in that: The following steps are involved: 10-15 parts of Lactobacillus plantarum MiuCul-01 (Lactobacillus plantarum); 5-10 parts of Lactobacillus acidophilus MiuLac-01 (Lactobacillus acidophilus); 3-7 parts of Lactobacillus casei MiaoTech-01 (Lactobacillus casei); 2-6 parts of Lactobacillus rhamnosus MiaoTech-11 (Lactobacillus rhamnosus); 8-12 parts of Streptococcus thermophilus MiaoPro-01 (Streptococcus thermophilus) are mixed uniformly, and subjected to two anaerobic cultures, followed by drying, anaerobic granulation, and anaerobic coating to obtain a Lactobacillus plantarum hypoglycemic preparation; During the two anaerobic cultures, a culture monitoring process is provided, which includes the following steps: S121: during the bacterial cell culture process, collection time points at the same time interval are set, and at each collection time point, sampling and quality inspection are performed on the bacterial cells in the culture box to obtain samples to be inspected, and data analysis is performed on the bacterial cell data to obtain the culture monitoring value; Obtain the concentration value of the cultured bacteria in the sample to be tested, and compare it with the concentration warning value and the concentration standard value; If the concentration value of the cultured bacteria is lower than the standard concentration value, a bacteria abnormality signal is generated; If the concentration value of the cultured bacteria is greater than or equal to the concentration standard value, and the concentration value of the cultured bacteria is less than the concentration warning value, a bacteria warning signal is generated; If the concentration value of the cultured bacteria is greater than or equal to the concentration warning value, a normal bacteria signal is generated; When there are no abnormal bacterial signals and there are bacterial warning signals or normal bacterial signals, culture risk monitoring and analysis is performed; The process of developing risk monitoring analysis is as follows: By formula The culture monitoring value KN is obtained, wherein s1 and s2 are preset proportional coefficients, KZ is expressed as the abnormal number ratio, and NZ is expressed as the abnormal degree ratio; S122: Acquire the culture monitoring value during the bacterial culture process, and judge the risk level of the bacterial culture situation based on the culture monitoring value. If the culture situation shows a high risk, generate a culture repair signal; If the culture monitoring value is greater than the culture monitoring threshold, a culture repair signal is generated; S123: based on the generated culture repair signal, obtaining suspected impact data causing a high risk of bacterial culture, wherein the suspected impact data includes an oxygen content value of the incubator, and performing data processing on the suspected impact data to obtain a similarity overlap ratio; Obtain the incubator oxygen content value at the acquisition time point and compare it with the oxygen content threshold value; If the oxygen content value is less than the oxygen content threshold, a normal oxygen content signal is generated; If the oxygen content value is greater than or equal to the oxygen content threshold, an oxygen content abnormality signal is generated; The abnormal oxygen content signal or the normal oxygen content signal generated at the same collection point time is compared and analyzed with the normal bacterial cell signal or the bacterial cell warning signal, respectively. If the abnormal oxygen content signal and the bacterial cell warning signal, or the normal oxygen content signal and the bacterial cell normal signal are generated at the same time, the collection point time is marked as a coincident collection point; Obtain the number of overlapping acquisition points, and perform ratio processing on the number of overlapping acquisition points and the number of all acquisition time points to obtain a similarity overlap ratio; S124: judging whether the cultured bacterial concentration warning is caused by abnormal oxygen content based on the similarity overlap ratio, and generating an impact signal if it is caused by abnormal dissolved oxygen concentration; If the similarity overlap ratio is greater than or equal to the similarity overlap ratio threshold, an influence signal is generated; S125: sending a control instruction based on the impact signal to control the ventilation frequency of the incubator; The process of obtaining the abnormal number ratio KZ is as follows: Obtain the number of samples that generate bacterial warning signals among the samples to be tested, and perform ratio processing on it with the total number of samples to be tested to obtain the abnormal number ratio, which is marked as KZ; The process of obtaining the abnormality ratio NZ is as follows: Obtain the bacterial concentration value of the sample that generates a bacterial warning signal, perform subtraction processing on it and the concentration warning value and take the absolute value to obtain the concentration warning difference, sum and average the concentration warning differences of all samples that generate bacterial warning signals to obtain the concentration warning mean, and perform ratio processing on it and the concentration warning value to obtain the abnormality ratio, which is marked as NZ.

2. The method for preparing a Lactobacillus plantarum hypoglycemic preparation according to claim 1, characterized in that: The process of regulating the ventilation frequency of the incubator is as follows: Extract the bacterial early warning signal collection time interval corresponding to the maximum concentration value of the cultured bacteria, obtain the start time and end time of the bacterial early warning signal collection time interval, and then use the start time as the base point to collect oxygen content values ​​at each time point in sequence, determine the time point when the oxygen content normal signal turns into the oxygen content abnormal signal, and record it as the reference time point; By formula The ventilation frequency control value TH is calculated; wherein, PY is the preset ventilation frequency of the bacteria during the culture process, TS represents the starting time of the bacteria warning signal collection time interval, TJ represents the end time of the bacteria warning signal collection time interval, and TC represents the reference time point.

3. The method for preparing a Lactobacillus plantarum hypoglycemic preparation according to claim 2, characterized in that: The process of obtaining the bacterial early warning signal collection time interval is as follows: When the impact signal is obtained, all adjacent bacterial early warning signals are combined and processed to obtain the bacterial early warning signal collection time interval.

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