LED light formulations for synergistic improvement of resistance, yield and quality of Anoectochilus roxburghii and their applications

Abstract

This invention relates to the field of medicinal plant cultivation technology, and particularly to an LED light formula for synergistically improving the resistance, yield, and quality of *Anoectochilus roxburghii* (a type of orchid) and its application. This invention utilizes LED blue, red, and far-red light combinations to treat transplanted *Anoectochilus roxburghii*. The treated plants showed significantly increased antioxidant enzyme activity and lignin content compared to the control group, and the progression of stem rot was delayed, with a significant decrease in incidence and disease index. The proposed LED light formula has a stable yield effect in *Anoectochilus roxburghii* production and helps increase the yield of dried *Anoectochilus roxburghii*. The proposed LED light formula also promotes the accumulation of polysaccharides, the effective active ingredient in *Anoectochilus roxburghii*, and can facilitate the in-depth development of *Anoectochilus roxburghii* polysaccharide products. This invention can achieve synergistic improvement in disease resistance, biomass accumulation, and polysaccharide content of *Anoectochilus roxburghii*.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of medicinal plant cultivation, and particularly relates to an LED light formula for synergistically improving the resistance, yield and quality of Anoectochilus roxburghii and application thereof. BACKGROUND

[0002] Anoectochilus roxburghii (Wall.) Lindl. is a rare and precious medicinal plant with medicinal, edible and ornamental values, rich in polysaccharides, flavonoids, sterols and other chemical active ingredients, and has the effects of clearing heat, soothing liver and detoxifying, and tonifying. Due to the destruction of the natural environment, overexploitation, and low seed germination rate and slow growth and development, the wild resources of Anoectochilus roxburghii have been listed in the National Second-class Protected Plant List. In recent years, the demand for Anoectochilus roxburghii in the domestic and foreign markets is increasing, and artificial planting is the only way to protect the wild resources of Anoectochilus roxburghii and promote the sustainable development of the Anoectochilus roxburghii industry. At present, the planting modes of Anoectochilus roxburghii mainly include facility cultivation and wild-like cultivation under forest, and the seedlings are mostly tissue culture seedlings. However, due to the characteristics of tissue culture seedlings, such as strict requirements for environmental factors, there are problems such as weak disease resistance, unstable yield and reduced quality in the process of artificial planting of Anoectochilus roxburghii, and therefore, creating an environment suitable for the growth of Anoectochilus roxburghii after transplanting is the key to successful cultivation.

[0003] Light, as one of the most important environmental factors affecting the growth and development of plants, is an essential factor in the artificial planting of Anoectochilus roxburghii. Wild Anoectochilus roxburghii mostly grows under forest with a shading degree of 60-80%. Correspondingly, Niu et al. (2020) reduced or controlled the light intensity reaching the canopy of Anoectochilus roxburghii by using shading nets or artificial light sources, which to some extent optimized the cultivation environment of Anoectochilus roxburghii. However, the influence of the proportion of different wave bands of light in the light on the growth of Anoectochilus roxburghii after transplanting was ignored in these studies or practices. It is of great significance to explore the optimal light spectrum formula suitable for Anoectochilus roxburghii after transplanting for the cultivation of Anoectochilus roxburghii with high resistance, yield and quality. SUMMARY

[0004] The technical problem to be solved by the present application is to provide an LED light formula for synergistically improving the resistance, yield and quality of Anoectochilus roxburghii in the cultivation of Anoectochilus roxburghii.

[0005] In order to solve the above technical problems, the technical scheme adopted by the present application is as follows:

[0006] The ratio of the blue light irradiance to the red light irradiance is 1.45-1.55, and the ratio of the red light irradiance to the far-red light irradiance is 0.47-0.50.

[0007] Further, in the above-mentioned LED light formula for synergistically improving the resistance, yield and quality of Anoectochilus roxburghii, the irradiance in the range of 400-700 nm is 12-14 W·m -2 , the photosynthetic photon flux density is 50-60 µmol·m -2 ·s -1 , the irradiance of far-red light in the range of 701-800 nm is 10-12 W·m -2 , and the far-red light photon flux density is 60-70 µmol·m -2 ·s -1 .

[0008] Further, in the above-mentioned LED light formula for synergistically improving the resistance, yield and quality of Anoectochilus roxburghii, the irradiance of blue light in the range of 400-500 nm is 7.83 W·m -2 , the irradiance of red light in the range of 601-700 nm is 5.35 W·m -2 , and the ratio of the irradiance of blue light to the irradiance of red light is 1.46.

[0009] Further, in the above-mentioned LED light formula for synergistically improving the resistance, yield and quality of Anoectochilus roxburghii, the irradiance of red light in the range of 601-700 nm is 5.35 W·m -2 , the irradiance of far-red light in the range of 701-800 nm is 11.0 W·m -2 , and the ratio of the irradiance of red light to the irradiance of far-red light is 0.49.

[0010] The present application also relates to the application of the above-mentioned LED light formula for synergistically improving the resistance, yield and quality of Anoectochilus roxburghii in the cultivation method of Anoectochilus roxburghii, which comprises the following steps: after the tissue culture seedlings of Anoectochilus roxburghii are hardened, they are transplanted into soil, the cultivation temperature is 22-24℃, the relative humidity is 70-80%, and the above-mentioned LED light formula is used for the illumination condition.

[0011] The present application also protects the Anoectochilus roxburghii product obtained under the above-mentioned LED light formula for synergistically improving the resistance, yield and quality of Anoectochilus roxburghii, which has high antioxidant enzyme activity, high lignin content, good stem rot resistance and high polysaccharide content.

[0012] The present application also protects the Anoectochilus roxburghii product obtained by the above-mentioned cultivation method of Anoectochilus roxburghii, which has high antioxidant enzyme activity, high lignin content, good stem rot resistance and high polysaccharide content.

[0013] The beneficial effects of this invention are as follows: This invention utilizes different combinations of LED blue, red, and far-red light formulations to treat transplanted *Anoectochilus roxburghii*. The antioxidant enzyme activity and lignin content of the treated plants were significantly increased compared to the control group. The progression of stem rot was delayed, and the incidence and disease index were significantly reduced, achieving a disease prevention effect of 52.3%. This indicates that the LED light formulation proposed in this invention can effectively control the occurrence and development of stem rot in *Anoectochilus roxburghii* production. Compared to the control group, the biomass accumulation in the leaves and stems of the treated plants increased by 1.70%–24.9% to varying degrees, and the dry weight was significantly increased by 11.6%–16.5%. This indicates that the LED light formulation proposed in this invention has a stable yield effect in *Anoectochilus roxburghii* production and helps increase the yield of dried *Anoectochilus roxburghii*. After four months of growth in the treated groups, the polysaccharide content in the leaves and stems of the *Anoectochilus roxburghii* plants increased by 12.2%–17.4% compared to the control group. This indicates that the LED light formulation proposed in this invention helps accumulate the effective active ingredient polysaccharide in *Anoectochilus roxburghii*, which can promote the in-depth development of *Anoectochilus roxburghii* polysaccharide products. This invention can achieve synergistic improvement of disease resistance, biomass accumulation and polysaccharide content in Anoectochilus roxburghii, laying the foundation for the future mass production of highly resistant, high-yielding and high-quality Anoectochilus roxburghii, and providing new ideas for optimizing Anoectochilus roxburghii cultivation techniques and environmental control measures. Attached Figure Description

[0014] Figure 1a This is a diagram of the experimental environment for light treatment in the cultivation method of Anoectochilus roxburghii in Embodiment 1 of the present invention;

[0015] Figure 1b This is a diagram of the controller panel of the light treatment device in the *Anoectochilus roxburghii* cultivation method of Embodiment 1 of the present invention;

[0016] Figure 2a This is the LED spectral distribution diagram of the CK group in the light treatment of the *Anoectochilus roxburghii* cultivation method in Embodiment 1 of the present invention;

[0017] Figure 2b This is a spectral distribution diagram of the LEDs in the FR group during light treatment in the cultivation method of Anoectochilus roxburghii in Embodiment 1 of the present invention;

[0018] Figure 3a This is a bar chart showing the peroxidase activity of *Anoectochilus roxburghii* plants treated with the CK and FR groups in Example 1 of this invention.

[0019] Figure 3b This is a bar chart showing the superoxide dismutase activity of *Anoectochilus roxburghii* plants treated with the CK and FR groups in Example 1 of this invention.

[0020] Figure 4a This is a bar chart showing the lignin content determination results of *Anoectochilus roxburghii* plants treated with the CK and FR groups in Example 1 of this invention.

[0021] Figure 4bThis is a diagram showing the lignin distribution measurement results of *Anoectochilus roxburghii* plants treated with the CK and FR groups in Example 1 of this invention.

[0022] Figure 5a This is a plan view of the *Anoectochilus roxburghii* plants treated in the CK and FR groups in Example 1 of this invention.

[0023] Figure 5b This is a top view of the *Anoectochilus roxburghii* plants treated in the CK and FR groups in Example 1 of the present invention.

[0024] Figure 5c This is a schematic diagram of the rootstock junction of *Anoectochilus roxburghii* plants treated in the CK and FR groups in Example 1 of the present invention.

[0025] Figure 6a This is a bar chart showing the biomass of *Anoectochilus roxburghii* plants treated in the CK and FR groups in Example 1 of this invention.

[0026] Figure 6b This is a bar chart showing the biomass of *Anoectochilus roxburghii* leaves after treatment in the CK and FR groups in Example 1 of this invention.

[0027] Figure 6c This is a bar chart of the biomass of *Anoectochilus roxburghii* stems after treatment in the CK and FR groups in Example 1 of this invention;

[0028] Figure 6d This is a bar chart showing the dry weight percentage of *Anoectochilus roxburghii* after treatment in the CK and FR groups in Example 1 of this invention.

[0029] Figure 7 This is a bar chart showing the polysaccharide content of *Anoectochilus roxburghii* after treatment in the CK and FR groups in Example 1 of this invention. Detailed Implementation

[0030] To explain in detail the technical content, objectives, and effects of the present invention, the following description is provided in conjunction with the embodiments and accompanying drawings.

[0031] LED is short for Light Emitting Diode. It has the characteristics of small size, light weight, long life and low energy consumption. In particular, LED light is pure and specific, and its spectral distribution is stable and easy to adjust, so it has become the preferred artificial light source for plant growth research.

[0032] In this invention, blue light refers to LED light with a wavelength range of 400-500nm, specifically 450nm, denoted by "B"; red light refers to LED light with a wavelength range of 601-700nm, specifically 660nm, denoted by "R"; and far-red light refers to LED light with a wavelength range of 701-800nm, specifically 730nm, denoted by "FR".

[0033] Unless otherwise specified, the experimental methods used in the following examples are conventional methods; the materials and reagents used are commercially available unless otherwise specified. The plant light analyzer used in this invention is a PLA-30 model, purchased from Hangzhou Yuanfang Optoelectronic Information Co., Ltd.; the LED light source is a 10-channel integrated chip lamp board covering ultraviolet / blue / green / yellow / red / far-infrared bands, provided by the Optoelectronic Agricultural Engineering and Technology Research Center of Fujian Agriculture and Forestry University, and the irradiance of each color LED can be controlled by a digital controller.

[0034] Example 1

[0035] A cultivation method for synergistically improving the resistance, yield, and quality of Anoectochilus roxburghii, including LED light treatment;

[0036] In this embodiment, a CK group and a FR group are set up. The CK group refers to the control group set up to simulate the proportion of different spectral bands in natural light, and the FR group refers to the experimental group set up with the improved spectral band proportions of this invention. The preset light conditions of the CK group and the FR group are achieved by adjusting the digital controller panel that communicates with the integrated chip lamp board. To avoid the influence of light sources from different treatments, each treatment is a self-contained cubic compartment, with five sides shielded by aluminum foil reflectors and one side shielded by aluminum foil reflective cloth, which facilitates the observation and sampling of plant growth status, as per reference. Figure 1a and Figure 1b The light source was positioned directly above the plant, and the irradiance within the 400–700 nm range of the plant canopy was measured to be 12–14 W·m² using a plant light analyzer. -2 The photosynthetic photon flux density is 50–60 μmol·m⁻¹ -2 ·s -1 LED spectral distribution as follows Figure 2a and Figure 2b As shown in Table 1, the average values ​​of the optical parameters are as follows.

[0037] Table 1

[0038]

[0039] In the preferred light formulation, the photosynthetic photon flux in the 400–700 nm range is 59.4 μmol·m⁻¹. -2 ·s -1 The blue light irradiance is 7.83 W·m. -2 The red light irradiance is 5.35 W·m. -2 The far-infrared photon flux density in the 701–800 nm range is 67.5 μmol·m⁻¹. -2 ·s -1 The far-infrared irradiance is 11.0 W·m. -2The irradiance ratio of blue light to red light is 1.46, and the irradiance ratio of red light to far-red light is 0.49.

[0040] Except for the light conditions, the cultivation and management conditions of the *Anoectochilus roxburghii* plants were the same for the CK and FR groups. In this example, 6-month-old tissue culture seedlings of the 'Hongxia' variety were selected as test materials. Healthy tissue culture seedlings with similar morphology and size were selected, cleaned, disinfected, and hardened off before being planted in cultivation trays (40cm long × 40cm wide × 8cm high) and placed under the aforementioned LED light treatment. The photocycle was 12 hours of light / 12 hours of darkness, with an ambient temperature of 22–24℃ and a relative humidity of 70–80% for 4 months of growth in a facility. All plants were watered with tap water every 3 days and fertilized with Flower Treasure Fertilizer No. 2 every 15 days. After the treatment period, plant samples were taken to measure relevant indicators.

[0041] In this embodiment, "PPFD" refers to photosynthetic photon flux density (in μmol). · s -1 · m -2 "E" refers to phytometric irradiance (measured in W). · m -2 The wavelength range is 400–700 nm; “PPFDF” refers to far-red photon flux density (unit: μmol). · s -1 · m -2 "EF" refers to far-red irradiance (measured in watts). · m -2 The wavelength range is 701–800 nm; “ER” refers to red-orange irradiance (unit: W). · m -2 The wavelength range is 601–700 nm; "EB" refers to blue-purple irradiance (measured in W). · m -2 The wavelength range is 400–500 nm; “R / FR” refers to the ratio of red irradiance (ER, 601–700 nm) to far-red irradiance (EF, 701–800 nm).

[0042] The measurement of some traits in the following experimental examples refers to the provisions of the Fujian Provincial Local Standard "Geographical Indication Product Yong'an Golden Thread Lotus" (DB35 / T 1388-2013).

[0043] The measurement methods used were mostly individual measurements and group visual inspections. Tools used for individual measurements included, but were not limited to, rulers, vernier calipers, electronic balances, and spectrophotometers. Methods for some indicators have been labeled. Six individual plants, representing the overall growth status of plants under both the CK and FR treatments, were selected for relevant indicator measurements.

[0044] Experimental Example 1

[0045] The antioxidant enzyme activity of *Anoectochilus roxburghii* plants treated with the CK and FR groups in Example 1 was determined using the following methods:

[0046] Enzyme extraction: Take 0.5g of fresh stem and leaf samples, add 5.0mL of 50mM phosphate buffer (pH 7.8, containing 1mM EDTA, 0.05% Triton X-100 and 2% polyvinylpyrrolidone) in three portions, and grind in liquid nitrogen until homogenized. Transfer the homogenate to 10ml centrifuge tubes and incubate at 4℃ and 12000rpm. · min -1 Centrifuge for 20 min and collect the supernatant to determine the activities of peroxidase POD and superoxide dismutase SOD;

[0047] Determination of peroxidase (POD) activity: 20 μL of supernatant was added to 3 mL of reaction solution containing 100 mM phosphate buffer (pH 6.0), guaiacol, and 30% hydrogen peroxide. The reaction solution was measured at 470 nm wavelength at 30-second intervals for 5 consecutive times, and the change in absorbance per minute was used to represent POD activity.

[0048] Determination of superoxide dismutase (SOD) activity: 20 μL of supernatant was added to 3 mL of reaction solution containing 50 mM sodium phosphate buffer (pH 7.8), 130 mM methionine, 0.75 mM nitroblue tetrazolium, 0.1 mM EDTA, and 0.02 mM riboflavin. The mixture was reacted under a 4000 lx white fluorescent lamp for 20 min, and SOD activity was measured at 560 nm. The results are as follows: Figure 3a and Figure 3b As shown, the light-colored bars represent CK, and the dark-colored bars represent FR.

[0049] The results showed that the POD activity of *Anoectochilus roxburghii* plants in the FR group was higher in both leaves and stems than that in the CK group, and the difference was significant in the stems (P<0.05). Figure 3a Similarly, SOD activity in the FR group was increased by 39.9% and 31.1% in leaves and stems, respectively, compared to the CK group, with the increase in leaves being highly significant (P<0.01). Figure 3bPOD and SOD can effectively scavenge reactive oxygen free radicals and are an important part of the plant's defense response mechanism. The FR group increased the antioxidant enzyme activity of Anoectochilus roxburghii plants, which helps them survive under stress.

[0050] Experiment Example 2

[0051] The lignin content and distribution of *Anoectochilus roxburghii* plants treated in the CK and FR groups of Example 1 were determined using the following methods:

[0052] Determination of lignin content: Weigh 0.005 g of crushed, dried plant sample into a 2 ml EP tube. A blank tube is included. Add 500 μL of 30% (v / v) acetyl bromide glacial acetic acid solution and 20 μL of 0.69 M perchloric acid to both the sample and blank tubes. Mix thoroughly and seal. Incubate at 80℃ for 40 min to allow for complete acetylation. Remove and allow to cool naturally to room temperature. Add 250 μL of 2 M NaOH and 250 μL of glacial acetic acid solution to terminate the reaction. Mix thoroughly and incubate at room temperature and 12000 rpm. · min -1 Centrifuge for 10 min, take 10 μL of the supernatant and dilute it to 1 mL with 990 μL of glacial acetic acid. Mix thoroughly and then use a 1 mL quartz cuvette to measure the absorbance at a wavelength of 280 nm using a UV spectrophotometer to calculate the lignin content.

[0053] Location of lignin distribution: Fresh *Anoectochilus roxburghii* plants were selected, and 5mm stem segments were cut transversely at the second internode of the rootstock junction. The segments were fixed with FAA fixative for at least 48 hours, dehydrated and cleared using gradient ethanol and xylene solutions, embedded in pure paraffin wax (melting point 56–58℃), and sectioned (10μm thickness) using a Leica automated microtome. The sections were then spread in a 40℃ water bath and baked in a 38℃ oven for at least 72 hours. The sections were placed on glass slides, stained with a few drops of 2% phloroglucinol ethanol solution for 3 minutes, followed by a few drops of 3mol / L hydrochloric acid for 1 minute. After mounting, the sections were observed, and the Wiesner staining method was used to reveal the location of G-lignin by staining with a purple-red color. The above results are as follows: Figure 4a and Figure 4b As shown.

[0054] The results showed that, compared with the CK group, the FR group increased lignin synthesis and accumulation in the leaves and stems of *Anoectochilus roxburghii* plants, with a highly significant difference of 0.01 in the stems (P<0.01). Figure 4a ); Phloroglucinol staining and microscopic observation results showed that, compared with the CK group, the FR group promoted the deposition of lignin in the stem nodes. Figure 4bLignin is an important secondary metabolite in plants, a crucial component of cell walls, and a hallmark of defense responses. The FR group increased the lignin content and deposition in *Anoectochilus roxburghii* plants, which helps enhance the host's defense capabilities.

[0055] Experimental Example 3

[0056] The resistance of *Anoectochilus roxburghii* plants treated with the CK and FR groups in Example 1 to stem rot was tested using the following methods:

[0057] Pathogen inoculation: The method of root drenching was used with a suspension of *Fusarium oxysporum* (the pathogen causing stem rot). Specifically, after culturing the pathogen on solid PDA medium at 28°C for 7 days, uncontaminated strains of suitable size were selected and transferred to liquid PDA medium (5mm diameter, 6-7 pieces / 100mL) using a hand-held punch. The medium was then incubated at 28°C and 150 rpm. · min -1 Incubate in a shaker for 3–5 days. After the bacterial culture turns purplish-red, filter and dilute appropriately with sterile distilled water, calculating a concentration of 1 × 10⁻⁶ using a Neubauer hemocytometer. 6 Prepare a spore suspension of 15 mL / mL for later use. Pipette the prepared pathogen suspension into the root-stem junction of the tested plants, adding 15 mL of suspension to each plant. Use sterile liquid PDA as a control group. Record the disease incidence after inoculation.

[0058] Evaluation of stem rot disease index of Anoectochilus roxburghii: 30 days after inoculation with pathogen, the number of diseased plants was counted, and the stem rot disease of Anoectochilus roxburghii was recorded according to the disease classification standard (Table 2). The incidence rate, disease index and disease control effect were calculated.

[0059] Table 2 Disease Severity of Anoectochilus roxburghii and Corresponding Symptoms

[0060]

[0061] Incidence rate (%) = (Number of infected plants / Total number of plants surveyed) × 100

[0062] Disease index (%) = Σ(Disease level × Number of plants at that level) / (Total number of plants × Most severe disease level) × 100

[0063] Disease prevention effect (%) = (Disease index of CK group - Disease index of FR group) / Disease index of CK group × 100

[0064] The measurement results are shown in Table 3:

[0065] Table 3. Disease incidence of stem rot in Anoectochilus roxburghii plants.

[0066]

[0067] The results showed that, compared with the CK group, the FR group treatment delayed the disease progression. Figure 5a , Figure 5b and Figure 5c The incidence rate decreased by 23.4%, the disease severity index decreased by 37.7%, and the disease prevention effect reached 52.3%.

[0068] Experiment Example 4

[0069] The *Anoectochilus roxburghii* plants treated in the CK and FR groups of Example 1 were subjected to overall growth-related trait measurements, including: plant height: the height from the base of the rhizome to the terminal bud; stem diameter: the diameter at the midpoint of the second stem segment counting upwards from the base; number of leaves: a leaf is counted as one leaf if it is more than half open; leaf area: the area of ​​all leaves on the plant, with each leaf calculated as leaf length (excluding the petiole, the vertical distance from the bottom to the highest point) × leaf width (the longest distance from the outermost edge of the left side of the leaf to the outermost edge of the right side) × 0.75. The results of these measurements are shown in Table 4.

[0070] Table 4. Statistical Table of Relevant Traits of Overall Plant Growth Vigor

[0071]

[0072] The results above indicate that there was no significant difference in the overall growth of plants in the CK group and the FR group. The plants of Anoectochilus roxburghii did not show a shade avoidance response under the low R / FR conditions in the FR group, which is conducive to the synergistic improvement of growth and defense.

[0073] Experimental Example 5

[0074] The biomass accumulation of *Anoectochilus roxburghii* plants treated in the CK and FR groups in Example 1 was measured using the following methods:

[0075] Each plant was manually separated into stems and leaves. After weighing the fresh weight using an electronic balance, the plants were placed in paper bags and blanched at 105℃ for 10 minutes, then dried at 70℃ until constant weight was achieved to determine the biomass accumulation in different parts. The dry weight percentage (%) was calculated as (plant dry weight / plant fresh weight) × 100. Results showed that the fresh and dry weights of the whole plant, leaves, and stems were increased to some extent in the FR group, but not significantly (P>0.05). Compared with the CK group, the FR group increased the dry weight percentage of leaves and stems, with a significant increase in leaves (P<0.05). Figure 6a , Figure 6b , Figure 6c and Figure 6d The light-colored bars are CK, and the dark-colored bars are FR.

[0076] The above results indicate that the FR group has the effect of stabilizing plant growth morphology and biomass accumulation in the production of Anoectochilus roxburghii, and also helps to increase the yield of dried Anoectochilus roxburghii.

[0077] Experimental Example 6

[0078] The effective component polysaccharide of *Anoectochilus roxburghii* plants treated in the CK and FR groups in Example 1 was determined using the following method:

[0079] 0.1 g of pulverized dried plant sample was extracted in 8 ml of deionized water (100 °C) for 20 min. After cooling, it was extracted at room temperature and 4500 rpm. · min -1 Centrifuge for 10 min (repeated 3 times), combine the supernatants containing sugar, and bring the total volume to 50 ml with deionized water. Take 1 ml of the supernatant and determine and calculate the soluble sugar content at 625 nm using the sulfuric acid-anthrone method, and determine and calculate the reducing sugar content at 540 nm using the 3,5-dinitrosalicylic acid (DNS) colorimetric method. Polysaccharide content = soluble sugar content - reducing sugar content. The results showed that compared with the CK group, the polysaccharide content in the leaves of *Anoectochilus roxburghii* increased by 12.2% and in the stems by 17.4% under the FR treatment, indicating that the FR group helps accumulate polysaccharides in *Anoectochilus roxburghii*, with a more significant increase in the stems. Figure 7 In this context, the light-colored bars represent CK, and the dark-colored bars represent FR.

[0080] The above description is merely an embodiment of the present invention and does not limit the patent scope of the present invention. Any equivalent modifications made based on the content of the present invention specification and drawings, or direct or indirect applications in related technical fields, are similarly included within the patent protection scope of the present invention.

Claims

1. LED light recipe for synergistically improving resistance, yield and quality of Ananas comosus, characterized in that, The irradiance in the range of 400-700 nm in the light recipe is 12-14 W·m -2 ; The photosynthetic photon flux in the 400–700 nm range is 59.4 µmol·m⁻¹. -2 ·s -1 The blue light irradiance in the 400-500nm range is 7.83 W·m. -2 The red light irradiance in the 601~700nm range is 5.35W·m. -2 The far-infrared photon flux density in the 701–800 nm range is 67.5 µmol·m⁻¹. -2 ·s -1 The far-infrared irradiance is 11.0 W·m. -2 The irradiance ratio of blue light to red light is 1.46, and the irradiance ratio of red light to far-red light is 0.

49.

2. Use of LED light recipe synergistically improving resistance, yield and quality of Ananas comosus in an Ananas comosus cultivation method, characterized in that, The Anoectochilus roxburghii cultivation method comprises the following steps: after the Anoectochilus roxburghii tissue culture seedlings are hardened, the Anoectochilus roxburghii tissue culture seedlings are transplanted into soil, the cultivation temperature is 22-24°C, the relative humidity is 70-80%, and the light condition adopts the LED light formula in claim 1.

3. The Anoectochilus roxburghii product obtained by the cultivation of claim 1 has high antioxidant enzyme activity, high lignin content, good stem rot resistance, and high polysaccharide content.

4. The Anoectochilus roxburghii product obtained by the cultivation method of claim 2 has high antioxidant enzyme activity, high lignin content, good stem rot resistance, and high polysaccharide content.

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