Alfalfa plant height and yield regulation protein MtFDa as well as coding gene and application thereof

Abstract

The invention discloses an alfalfa plant height and yield regulation protein MtFDa as well as a coding gene and application thereof. The invention belongs to the technical field of biology, and particularly relates to alfalfa plant height and yield regulation protein MtFDa as well as a coding gene and application thereof. The protein is any one of the following proteins: A1) a protein with an amino acid sequence as shown in SEQ ID No.2; a2) a protein which is obtained by substitution and / or deletion and / or addition of amino acid residues on the protein of A1), has 80% or more of identity with the protein of A1) and has the same function as the protein of A1); a3) a fusion protein obtained by connecting a protein tag to the N terminal or / and the C terminal of A1) or A2). The alfalfa biomass can be improved by inhibiting and reducing the expression of MtFDa, and the protein can be applied to alfalfa breeding.

Description

Technical Field

[0001] The invention belongs to the field of biotechnology, and in particular relates to alfalfa plant height and yield regulating protein MtFDa and a coding gene and application thereof. background Leguminosae Leguminosae ) There are about 700 genera and 18,000 species of plants, which is the third largest family after Asteraceae and Orchidaceae. It is widely distributed throughout the world and is an important source of food and oil for humans, as well as forage, green manure, medicinal materials and timber. It has important economic, ecological and biological values. However, due to the large genome size of most legume crops and the immature genetic transformation system, the research on functional genomics of legumes is limited. Medicago truncatula ( Medicago truncatula ) is an annual herb and grazing forage. It is an ideal model plant for genetics and genomics research in legumes due to its short growth cycle, small ploidy, small genome, self-pollination and nitrogen fixation.

[0002] The main goal of increasing alfalfa yield is to increase the vegetative body, which is to say, it involves the question of the time of transition from vegetative growth to reproductive growth. If this transition period can be delayed, the growth time of the vegetative body can be extended, thereby achieving the goal of increasing yield. Therefore, exploring the key regulatory genes of plant height and yield provides important resources for cultivating high-yield crops. Summary of the invention

[0003] The technical problem to be solved by the present invention is how to regulate the plant height and / or increase the plant yield.

[0004] In order to solve the problems existing in the prior art, the present invention provides a protein.

[0005] The protein provided by the present invention may be any of the following proteins: A1) a protein with an amino acid sequence as shown in SEQ ID No. 2; A2) a protein obtained by replacing and / or deleting and / or adding amino acid residues of the protein of A1) and having more than 75% identity with the protein of A1) and having the function of regulating plant height and yield; For example, a person skilled in the art can obtain a protein mutant having the same function as the amino acid sequence shown in SEQ ID No. 2 by replacing, deleting and / or adding one or more amino acids based on the amino acid sequence shown in SEQ ID No. 2 and conventional techniques in the art such as conservative substitution of amino acids without affecting its activity; A3) A fusion protein obtained by connecting a protein tag to the N-terminus or / and C-terminus of A1) or A2).

[0006] The protein described in A1) above is named MtFDa. The protein shown in SEQ ID No. 2 consists of 236 amino acid residues.

[0007] In order to facilitate purification or detection of the protein in A1), a tag protein may be connected to the amino terminus or carboxyl terminus of the protein consisting of the amino acid sequence shown in SEQ ID No. 2 in the sequence listing.

[0008] The above proteins can be artificially synthesized, or their encoding genes can be synthesized first and then expressed biologically.

[0009] The tag protein includes but is not limited to: GST (glutathione sulfhydryltransferase) tag protein, His6 tag protein (His-tag), MBP (maltose binding protein) tag protein, Flag tag protein, SUMO tag protein, HA tag protein, Myc tag protein, eGFP (enhanced green fluorescent protein), eCFP (enhanced cyan fluorescent protein), eYFP (enhanced yellow-green fluorescent protein), mCherry (monomeric red fluorescent protein) or AviTag tag protein.

[0010] Those skilled in the art can easily mutate the nucleotide sequence encoding the protein MtFDa of the present invention by using known methods, such as directed evolution or point mutation. Those artificially modified nucleotides having 75% or more identity with the nucleotide sequence of the protein MtFDa isolated by the present invention are all derived from the nucleotide sequence of the present invention and are equivalent to the sequence of the present invention as long as they encode the protein MtFDa and have the function of the protein MtFDa.

[0011] The above-mentioned 75% or more identity may be 80%, 85%, 90% or 95% or more identity.

[0012] Herein, identity refers to the identity of an amino acid sequence or a nucleotide sequence. The identity of an amino acid sequence or a nucleotide sequence can be determined using a homology search site on the Internet, such as the BLAST webpage on the NCBI homepage website. For example, in Advanced BLAST2.1, by using blastp as a program, setting the Expect value to 10, setting all Filters to OFF, using BLOSUM62 as a Matrix, setting the Gap existence cost, Per residue gap cost and Lambda ratio to 11, 1 and 0.85 (default values) respectively and searching for a pair of amino acid sequences or nucleotide sequences to calculate the identity, then the value (%) of the identity can be obtained.

[0013] Herein, the 80% or greater identity may be at least 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% identity.

[0014] Herein, the 90% or greater identity may be at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% identity.

[0015] In the above, the protein is derived from Medicago truncatula ( Medicago truncatula ).

[0016] The present invention also provides a biological material related to the above protein, and the biological material may be any one of the following: B1) a nucleic acid molecule encoding the protein described above; B2) an expression cassette containing the nucleic acid molecule described in B1); B3) a recombinant vector containing the nucleic acid molecule described in B1), or a recombinant vector containing the expression cassette described in B2); B4) a recombinant microorganism containing the nucleic acid molecule described in B1), or a recombinant microorganism containing the expression cassette described in B2), or a recombinant microorganism containing the recombinant vector described in B3); B5) a transgenic plant cell line containing the nucleic acid molecule described in B1) or a transgenic plant cell line containing the expression cassette described in B2); B6) transgenic plant tissue containing the nucleic acid molecule described in B1), or transgenic plant tissue containing the expression cassette described in B2); B7) a transgenic plant organ containing the nucleic acid molecule described in B1) or a transgenic plant organ containing the expression cassette described in B2); C1) a nucleic acid molecule that inhibits, reduces or silences the expression of the gene encoding the protein mentioned above; C2) expressing the gene encoding the nucleic acid molecule described in C1); C3) an expression cassette containing the coding gene described in C2); C4) a recombinant vector containing the coding gene described in C2) or a recombinant vector containing the expression cassette described in C3); C5) a recombinant microorganism containing the coding gene described in C2), or a recombinant microorganism containing the expression cassette described in C3), or a recombinant microorganism containing the recombinant vector described in C4); C6) a transgenic plant cell line containing the coding gene described in C2), or a transgenic plant cell line containing the expression cassette described in C3), or a transgenic plant cell line containing the recombinant vector described in C4); C7) transgenic plant tissue containing the coding gene described in C2), or transgenic plant tissue containing the expression cassette described in C3), or transgenic plant tissue containing the recombinant vector described in C4); C8) A transgenic plant organ containing the coding gene described in C2), or a transgenic plant organ containing the expression cassette described in C3), or a transgenic plant organ containing the recombinant vector described in C4).

[0017] In the above biological material, the nucleic acid molecule described in B1) may be the gene shown in E1) or E2) below: E1) The coding sequence is a cDNA molecule or a DNA molecule of SEQ ID No. 1; E2) The nucleotide sequence is a cDNA molecule or a DNA molecule of SEQ ID No. 2.

[0018] DNA molecule shown in SEQ ID No.1 (regulating plant height and yield traits) In the MtFD Gene) encodes the protein MtFDa whose amino acid sequence is SEQ ID No.2.

[0019] The nucleotide sequence shown in SEQ ID No. 1 is the nucleotide sequence of the gene (CDS) encoding the protein MtFDa.

[0020] The present invention In the MtFD The gene can be any nucleotide sequence that can encode the protein MtFDa. Considering the degeneracy of codons and the preference of codons of different species, those skilled in the art can use codons suitable for expression of a specific species as needed.

[0021] B1) The nucleic acid molecule may also include a nucleic acid molecule obtained by modifying the codon preference based on the nucleotide sequence shown in SEQ ID No. 3.

[0022] B1) The nucleic acid molecule may also include a nucleic acid molecule having a nucleotide sequence identity of more than 95% with the nucleotide sequence shown in SEQ ID No. 3 and derived from the same species.

[0023] The nucleic acid molecule described herein can be DNA, such as cDNA, genomic DNA or recombinant DNA; the nucleic acid molecule can also be RNA, such as gRNA, mRNA, siRNA, shRNA, sgRNA, miRNA or antisense RNA.

[0024] The vectors described herein are well known to those skilled in the art, including but not limited to: plasmids, bacteriophages (such as lambda phage or M13 filamentous phage, etc.), cosmids (i.e., cosmids), Ti plasmids or viral vectors.

[0025] Existing plant expression vectors can be used to construct In the MtFD The plant expression vector includes, but is not limited to, binary Agrobacterium vectors and vectors that can be used for plant microprojectile bombardment. The plant expression vector may also include the 3' non-translated region of the foreign gene, i.e., a polyadenylic acid signal and any other DNA fragments involved in mRNA processing or gene expression. The polyadenylic acid signal can guide the addition of polyadenylic acid to the 3' end of the mRNA precursor, such as but not limited to Agrobacterium crown gall induction (Ti) plasmid genes (such as carmine synthase Nos gene), plant genes (such as soybean storage protein gene) 3' end transcription non-translated regions have similar functions.

[0026] The microorganism described herein may be yeast, bacteria, algae or fungi. Among them, the bacteria may be from the genus Escherichia ( Escherichia ), Erwinia ( Erwinia ), Agrobacterium tumefaciens ( Agrobacterium ), Flavobacterium ( Flavobacterium ), Alcaligenes spp. Alcaligenes ), Pseudomonas spp. ( Pseudomonas ), Bacillus spp. ( Bacillus ) etc. Specifically, it can be Agrobacterium tumefaciens EHA105.

[0027] The present invention also provides a method for regulating plant height and yield, comprising regulating the activity and / or content of the above-mentioned protein in the target plant, or / and the expression level of the gene encoding the protein, to regulate plant height and increase yield.

[0028] In the above method, the regulation of the activity and / or content of the protein MtFDa in the target plant, or / and the expression level of the gene encoding the protein, comprises introducing into the recipient plant a gene encoding the protein that inhibits, reduces or silences the protein. In the MtFD , obtaining target plants with changed plant height and increased yield; In the MtFD The coding gene encodes the protein MtFDa.

[0029] The introduction refers to the introduction by recombinant means, including but not limited to Agrobacterium ( Agrobacterium )-mediated transformation, biolistic methods, electroporation, in planta technology, etc.

[0030] The present invention also provides the use of the above-mentioned protein MtFDa or a substance regulating the expression of a gene or a substance regulating the activity or content of the protein in any of the following: U1) Application in regulating plant height and yield; U2) Application in the preparation of products for regulating plant height and yield; U3) Application in breeding plants with increased plant height and yield; U4) Use in the preparation of products for cultivating plants with increased plant height and yield; U5) Application in plant breeding.

[0031] Herein, the substance that regulates the activity and / or content of the protein may be a substance that regulates the expression of a gene that encodes the protein MtFDa.

[0032] In the above application, the substance that regulates the expression of the gene or the substance that regulates the activity or content of the protein may be a biological material related to the protein, and the biological material may be the biological material described above.

[0033] In the above-mentioned applications and methods, the regulation may be increasing, enhancing or up-regulating.

[0034] In the above-mentioned uses and methods, the regulation may be inhibition, reduction or silencing.

[0035] Herein, the substance that regulates gene expression may be a substance that performs at least one of the following six types of regulation: 1) Regulation at the transcriptional level of the gene; 2) regulation after the transcription of the gene (i.e., regulation of the splicing or processing of the primary transcript of the gene); 3) Regulation of RNA transport of the gene (i.e., regulation of the transport of mRNA of the gene from the nucleus to the cytoplasm); 4) Regulation of the translation of the gene; 5) Regulation of mRNA degradation of the gene; 6) Post-translational regulation of the gene (i.e., regulation of the activity of the protein translated from the gene).

[0036] Herein, the regulation of the expression of the coding gene of the protein may be inhibition, reduction or down-regulation of the coding gene expression. Inhibition, reduction or down-regulation of the coding gene expression may be achieved by T-DNA insertion, gene knockout or gene silencing.

[0037] In order to facilitate the identification and screening of transgenic cells or plants, the recombinant expression vector used can be processed, such as adding genes encoding enzymes or luminescent compounds that can be expressed in plants and can produce color reactions, antibiotic markers with resistance, or chemical agent resistance marker genes, etc. It is also possible to directly screen transformed plants by adversity without adding any selective marker genes.

[0038] The plant obtained by the above method can be a transgenic plant, or a plant obtained by conventional breeding techniques such as hybridization. In the above method, the transgenic plant is understood to include not only first-generation to second-generation transgenic plants, but also their progeny. For transgenic plants, the gene can be propagated in the species, and the gene can also be transferred into other varieties of the same species using conventional breeding techniques, especially including commercial varieties. The transgenic plant includes seeds, callus, complete plants and cells.

[0039] The present invention also provides a method for cultivating plants with improved plant height and yield, comprising: 1) inhibiting, reducing or silencing the expression level of the gene encoding the protein mentioned above in the target plant, or / and inhibiting, reducing or silencing the activity and / or content of the gene encoding the protein mentioned above, to obtain plants with improved plant height and yield; 2) Increasing, enhancing or up-regulating the expression level of the gene encoding the protein mentioned above in the target plant, or / and increasing, enhancing or up-regulating the activity and / or content of the gene encoding the protein mentioned above, to obtain a plant with reduced plant height and yield.

[0040] In a specific embodiment, a method for cultivating plants with increased plant height and yield comprises the following steps: inhibiting the expression of a nucleic acid molecule encoding an MtFDa protein in a target plant to obtain a transgenic plant with increased plant height and yield. The inhibition of the expression of a nucleic acid molecule encoding an MtFDa protein in a target plant can be achieved by introducing a T-DNA targeting a nucleic acid molecule encoding an MtFDa protein into the target plant.

[0041] The knockout vector may be a gene editing vector.

[0042] As an embodiment of the present invention, the method for cultivating plants with increased plant height and yield comprises the following steps: (1) Constructing a protein containing inhibitors In the MtFD Gene editing vectors for gene expression or insertion of T-DNA molecule sequences; (2) introducing the gene editing vector or T-DNA molecule sequence (Tnt1) constructed in step (1) into the plant; (3) Plants with increased plant height and yield are obtained through screening and identification.

[0043] In the present invention, the purpose of plant breeding includes cultivating plants with increased / reduced plant height and / or increased / reduced yield.

[0044] In the above application or method, the plant is any one of the following: N1) Dicotyledonous plants: N2) Plants of the order Rosales; N3) Leguminosae; N4) Medicago; N5) Medicago truncatula.

[0045] In a specific embodiment, the Medicago truncatula may be: Medicago truncatula R108 or a mutant in the media . BRIEF DESCRIPTION OF THE DRAWINGS

[0046] tú1 for In the MtFD Gene structure diagram and mtfda-3 (NF10125), mtfda-4 (NF20902) mutant TNT1 Schematic diagram of the insertion position of the gene (A) and the electrophoresis results of the products amplified using the primer pair MtFDa-F / MtFDa-R (B).

[0047] tú2 Medicago truncatula R108 wild type (WT) and Medicago truncatula in the media Phenotype of the mutant at the initial flowering stage of R108 (A) and biomass statistics (B).

[0048] tú3 Medicago truncatula R108 wild type (WT) and Medicago truncatula in the media Mutants in in the media Phenotype of the mutant at the initial flowering stage (A) and biomass statistics (B). DETAILED DESCRIPTION

[0049] The present invention is further described in detail below in conjunction with specific embodiments, and the examples provided are only for illustrating the present invention, rather than for limiting the scope of the present invention. The examples provided below can be used as a guide for further improvements by those of ordinary skill in the art, and do not constitute a limitation of the present invention in any way.

[0050] The experimental methods in the following examples, unless otherwise specified, are all conventional methods, and are performed according to the techniques or conditions described in the literature in the field or according to the product instructions. The materials, reagents, etc. used in the following examples, unless otherwise specified, can all be obtained from commercial channels.

[0051] Medicago truncatula R108 and in the The mutants are provided by The Nobel Foundation: https: / / www .nobelprize .org / thenobel-prize-organisation / the-nobel- foundation / provide.

[0052] In the following embodiments in theThe numbers in the Medicago truncatula Tnt1 insertion mutant library are: mtfda-3 (NF10125), mtfda-4 (NF20902). According to the information recorded in the Medicago truncatula Tnt1 insertion mutant library, the Tnt1 insertion in the mutant was determined. In the MtFD Location on the genome.

[0053] The following examples were processed using SPSS11.5 statistical software, and the experimental results were expressed as mean ± standard deviation. One-way ANOVA test was used, and P < 0.01 (**) indicated a significant difference, and P < 0.001 (***) indicated a very significant difference.

[0054] Embodiment 1, In the MtFD Confirmation of linkage relationship with biomass increase phenotype and its encoding gene In the MtFD Acquisition 1. According to Medicago truncatula TNT1 The flanking sequence of NF10125 published on the website (https: / / medicago-mutant.noble.org / mutant / ) was used to design the corresponding primers. In the MtFD -Insertion-F and In the MtFD -Insertion-R was combined with the primers of Tnt1 (LTR6 or LTR31) to screen mtfda-4 The Tnt1 insertion site with co-segregated mutant phenotypes was used to preliminarily identify the target gene.

[0055] LRT31-F: 5'-CTCCTCTCGGGGTCGTGGTT-3'; LTR6-R: 5'-GCTACCAACCAAACCAAGTCAA-3'; MtFDa-Insertion-F: 5'-TCCTTGACACCTTTCTCACT-3'; MtFDa-Insertion-R: 5'-ACGGTGAAGCATTTACGAAA-3'.

[0056] Discovery of a gene linked to the mutant In the MtFD , NF10125 mutant TNT1 Inserted into the first exon of the gene. To further prove the function of the mutant, another mutant of the gene was purchased: NF20902 ( mtfda-4 , TNT1 Inserted in the first exon) tú1 Middle A).

[0057] Phenotypic analysis showed: mtfda-3 and mtfda-4 All showed phenotypes of increased plant height and yield. In the MtFD Linkage type with the above phenotypes.

[0058] 2. Total RNA was extracted from the apical meristem (Apex) of Medicago truncatula R108 (wild type) after phase conversion (branch buds converted into flower buds), reverse transcribed into cDNA as a template, and amplified using primer pair MtFDa-F / MtFDa-R.

[0059] MtFDa-F: 5'-ATGGAAGAAGTTTGGAAAGACATAAAC-3'; MtFDa-R: 5'- TCAAAAGGATGATGTTCGATATAAG-3'.

[0060] The obtained PCR amplification products ( tú1 B) was sequenced to obtain the coding region sequence of the target gene (nucleotide sequence as shown in SEQ ID No.1), encoding the protein shown in SEQ ID No.2. It consists of 236 amino acid residues. The protein shown in SEQ ID No.2 of the sequence list was named MtFDa, and the coding gene of MtFDa was named In the MtFD Gene. In the MtFD The CDS of the gene was obtained after comparison and verification on the alfalfa website (http: / / blast.jcvi.org / Medicago-Blast / ) In the MtFD The genomic sequence of the gene (the nucleotide sequence is shown in SEQ ID No.3).

[0061] 3. Extract wild-type R108 and in the media The total RNA of the two mutants was reverse transcribed into cDNA as template. In the MtFD The specific primers MtFDa-F / MtFDa-R were used to amplify In the MtFD The primer pair consisting of primers RT-MtActin-F / RT-MtActin-R was used to amplify the internal reference gene of Medicago truncatula MtActin , Real-time PCR (RT-PCR) was performed.

[0062] The results showed that the two mutants ( mtfda-3、mtfda-4 ) was not detected In the MtFD Gene expression ( tú2 B), further explanation In the MtFD The deletion of the gene leads to an increase in the biomass of Medicago truncatula, and the MtFDa protein plays an important role in the regulation of plant height and yield of Medicago truncatula.

[0063] Embodiment 2, in the media Obtaining mutants and phenotypic analysis By screening the Medicago truncatula Tnt1 insertion mutant library, we purchased the mutant mtfda-3 (NF10125), mtfda-4 (NF20902). Take 30 mutants ( mtfda-3、mtfda-4 ) and Medicago truncatula R108 seeds were vernalized at 4 ℃ for 7 days, planted in nutrient soil, and cultured in an artificial climate chamber (16 h light / 8 h dark) to observe their phenotypes.

[0064] Phenotype collection and biomass statistics were carried out at the early flowering stage of R108. mtfda-3、mtfda-4、 10 strains were counted for WT and 10 strains for WT. tú2 As shown in A: in the media The mutant was significantly higher than the wild type Medicago truncatula R108, and its biomass was significantly greater than the wild type Medicago truncatula R108; in the The mutants were phenotyped at the initial flowering stage ( tú3 A) and biomass statistics ( tú3 Middle B), tfda-3、mtfda-4、 WT and 10 strains were counted, and the results showed that compared with the wild type, in the media The mutant plants were significantly taller and had a significantly greater biomass than the wild type.

[0065] The present invention has been described in detail above. For those skilled in the art, without departing from the purpose and scope of the present invention, and without the need to carry out unnecessary experimental conditions, the present invention can be implemented in a wide range under equivalent parameters, concentrations and conditions. Although the present invention provides specific embodiments, it should be understood that the present invention can be further improved. In a word, according to the principles of the present invention, the application is intended to include any changes, uses or improvements to the present invention, including departure from the disclosed scope in the application, and changes made with conventional techniques known in the art.

Claims

1. A protein, wherein the protein is any of the following: A1) a protein with an amino acid sequence as shown in SEQ ID No. 2; A2) a protein obtained by replacing and / or deleting and / or adding amino acid residues of the protein of A1) and having more than 80% identity with the protein of A1) and having the same function; A3) A fusion protein obtained by connecting a protein tag to the N-terminus or / and C-terminus of A1) or A2).

2. The protein according to claim 1, characterized in that: The protein is derived from alfalfa.

3. A biological material related to the protein according to claim 1 or 2, wherein the biological material is any one of the following: B1) A nucleic acid molecule encoding the protein according to claim 1 or 2; B2) an expression cassette containing the nucleic acid molecule described in B1); B3) a recombinant vector containing the nucleic acid molecule described in B1), or a recombinant vector containing the expression cassette described in B2); B4) a recombinant microorganism containing the nucleic acid molecule described in B1), or a recombinant microorganism containing the expression cassette described in B2), or a recombinant microorganism containing the recombinant vector described in B3); B5) a transgenic plant cell line containing the nucleic acid molecule described in B1) or a transgenic plant cell line containing the expression cassette described in B2); B6) transgenic plant tissue containing the nucleic acid molecule described in B1), or transgenic plant tissue containing the expression cassette described in B2); B7) a transgenic plant organ containing the nucleic acid molecule described in B1) or a transgenic plant organ containing the expression cassette described in B2); C1) a nucleic acid molecule that inhibits, reduces or silences the expression of a gene encoding a protein as claimed in claim 1 or 2; C2) expressing a gene encoding the nucleic acid molecule described in C1); C3) an expression cassette containing the coding gene described in C2); C4) a recombinant vector containing the coding gene described in C2) or a recombinant vector containing the expression cassette described in C3); C5) a recombinant microorganism containing the coding gene described in C2), or a recombinant microorganism containing the expression cassette described in C3), or a recombinant microorganism containing the recombinant vector described in C4); C6) a transgenic plant cell line containing the coding gene described in C2), or a transgenic plant cell line containing the expression cassette described in C3), or a transgenic plant cell line containing the recombinant vector described in C4); C7) transgenic plant tissue containing the coding gene described in C2), or transgenic plant tissue containing the expression cassette described in C3), or transgenic plant tissue containing the recombinant vector described in C4); C8) A transgenic plant organ containing the coding gene described in C2), or a transgenic plant organ containing the expression cassette described in C3), or a transgenic plant organ containing the recombinant vector described in C4).

4. The biomaterial according to claim 3, characterized in that B1) The nucleic acid molecule is the gene shown in E1) or E2) below: E1) The coding sequence is a cDNA molecule or a DNA molecule of SEQ ID No. 1; E2) The nucleotide sequence is a cDNA molecule or a DNA molecule of SEQ ID No.

3.

5. A method for regulating plant height and yield, characterized in that: The method comprises regulating the activity and / or content of the protein described in claim 1 or 2, or / and the expression level of the gene encoding the protein described in claim 1 or 2 in the target plant to regulate the plant height and yield.

6. The method according to claim 5, characterized in that The regulating the activity and / or content of the protein described in claim 1 or 2 in the target plant, or / and the expression level of the gene encoding the protein described in claim 1 or 2, comprises introducing a gene encoding the protein that inhibits, reduces or silences the protein into the recipient plant, thereby obtaining a target plant having a higher plant height and yield than the recipient plant; the encoding gene encodes the protein described in claim 1 or 2.

7. Application, characterized in that, The application is any of the following: U1) Use of the protein or gene expression regulating substance of claim 1 or 2 or the substance regulating the activity or content of the protein in regulating plant height and yield; U2) Use of the protein or gene expression regulating substance of claim 1 or 2 or the substance regulating the activity or content of the protein in the preparation of a product for regulating plant height and yield; U3) Use of the protein or gene expression regulating substance of claim 1 or 2 or the substance regulating the activity or content of the protein in cultivating plants with altered plant height and yield; U4) Use of the protein or gene expression regulating substance according to claim 1 or 2 or the substance regulating the activity or content of the protein in the preparation of products for cultivating plants with increased plant height and yield; U5) Use of the protein or gene expression regulating substance according to claim 1 or 2 or the substance regulating the activity or content of the protein in plant breeding.

8. The use according to claim 7, characterized in that: The substance that regulates gene expression or the substance that regulates the activity or content of the protein is a biological material related to the protein, and the biological material is the biological material according to claim 3 or 4.

9. A method for cultivating plants with altered plant height and yield, comprising: 1) Inhibiting or reducing or silencing the expression of the gene encoding the protein of claim 1 in the target plant, or / and inhibiting or reducing or silencing the activity and / or content of the gene encoding the protein of claim 1, to obtain a plant with increased plant height and yield; 2) Increasing, enhancing and / or up-regulating the expression level of the gene encoding the protein described in claim 1 in the target plant, or / and increasing, enhancing and / or up-regulating the activity and / or content of the gene encoding the protein described in claim 1, to obtain a plant with reduced plant height and yield.

10. The method according to any one of claims 5, 6 or 9, or the use according to claim 7 or 8, characterized in that: The plant is any one of the following: N1) Dicotyledonous plants: N2) Plants of the order Rosales; N3) Leguminosae; N4) Medicago; N5) Medicago truncatula.

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