Molecular marker for stripe rust resisting site of wheat 7AL chromosome and application of molecular marker

Abstract

The invention relates to a molecular marker of a stripe rust resisting site of a wheat 7AL chromosome in the field of crop genetic breeding and application of the molecular marker. The SSR molecular marker primer group of wheat is characterized in that the SSR molecular marker primer group is composed of a primer F and a primer R, the primer F is a DNA molecule with a nucleotide sequence as shown in SEQ ID No.1, and the primer R is a DNA molecule with a nucleotide sequence as shown in SEQ ID No.2. The molecular marker provided by the invention has the characteristics of rapidness, accuracy and high efficiency, can be used for screening plants with stripe rust resistance QTL QYr.cau-7AL, provides technical support for wheat stripe rust character improvement and disease resistance breeding by utilizing molecular marker-assisted selective breeding, and has important application in wheat stripe rust resistance molecular marker-assisted breeding and resistance improvement.

Description

Technical Field

[0001] The invention relates to a molecular marker of a wheat 7AL chromosome stripe rust resistance site in the field of crop genetic breeding and an application thereof. Background Art

[0002] Wheat (Triticum aestivum L.) is the largest crop planted worldwide, providing about 20% of the world's total food energy. Maintaining high and stable wheat yields is crucial to national economy and people's livelihood and global food security (Hickey et al., 2019). However, stripe rust caused by Puccinia striiformis Westend.f.sp.tritici Erikss. has been a serious threat to wheat production safety. Using disease-resistant varieties to control diseases has the advantages of being economical, effective and environmentally friendly.

[0003] Extensive research has been conducted on wheat stripe rust resistance resources at home and abroad. So far, 86 stripe rust resistance genes (Yr1-Yr86) have been officially named, and hundreds of disease resistance quantitative trait loci (QTL) have been reported. Their resistance to stripe rust is divided into two categories: seedling resistance or all-stage resistance (ASR), and adult plant resistance (APR). Wheat varieties with all-stage resistance show an immune response without visible symptoms from the first leaf to the dryness of leaves at maturity, or show high resistance and necrotic spots and / or chlorotic spots. This type of disease resistance is usually controlled by a single gene and has been widely used in the breeding of disease-resistant varieties. However, many genes that control this type of disease resistance have been proven to be race-specific, that is, they are only effective for a few races, such as Yr9 and Yr26, and are easily overcome by new toxic races of wheat stripe rust and become ineffective. Wheat varieties with adult-stage resistance are susceptible to the disease during the seedling growth stage, but have milder stripe rust than susceptible control varieties in the adult stage, for example, lower severity or smaller area under the disease progress curve (AUDPC), showing quantitative resistance characteristics. This type of disease resistance is usually controlled by QTL. In the literature, it is often believed that genes / QTLs controlling quantitative disease resistance are not race-specific, but there are a few exceptions.

[0004] However, there is still a lack of available stripe rust resistance genes / resources in wheat production and breeding. The main reasons include: (1) Most of the 86 Yr genes mentioned above have no disease resistance against new dominant stripe rust species due to their species-specificity; (2) Yr genes from other species are often linked to some undesirable traits (such as late maturity) (linkage drag) and are not conducive to their application in disease resistance breeding, or are suppressed in the genetic background of common wheat; (3) Most of the disease resistance QTLs reported at home and abroad have small disease resistance effects (referred to as "minor effect QTLs") and / or are unstable, that is, they show disease resistance in some experiments, but cannot significantly reduce the disease in other experiments / environments; (4) Even those "major effect QTLs" with large disease resistance effects are difficult to transfer or aggregate into new varieties due to the lack of tightly linked DNA markers. Therefore, on the one hand, it is necessary to continue to screen new disease resistance resources, and on the other hand, the disease resistance QTLs that have been located should be applied to the breeding of new varieties as soon as possible. The efficiency of disease resistance breeding can be improved by obtaining DNA markers closely linked to QTLs and applying marker-assisted selection to accelerate QTL aggregation. Summary of the invention

[0005] The main problem to be solved by the invention is how to identify the stripe rust resistance of wheat and carry out disease-resistant wheat breeding.

[0006] In order to solve the above problems, the present invention provides a wheat SSR molecular marker primer set.

[0007] The wheat SSR molecular marker primer set provided by the present invention consists of primer F and primer R, wherein the primer F is a DNA molecule having a nucleotide sequence as shown in SEQ ID No.1, and the primer R is a DNA molecule having a nucleotide sequence as shown in SEQ ID No.2.

[0008] The IWGSC_RefSeq_v1.0 sequence of the genome of the common wheat variety Chinese Spring is used as the reference genome, and the SSR molecular marker is located at positions 671,733,466 to 671,733,723 of chromosome 7A of the wheat genome (RefSeq v1.0).

[0009] The present invention also provides a kit, which comprises the SSR molecular marker primer set described above.

[0010] The present invention also provides a DNA chip, which comprises the SSR molecular marker primer set described above.

[0011] The present invention also provides any of the following applications of the aforementioned SSR molecular marker primer set, and / or the aforementioned kit, and / or the aforementioned DNA chip:

[0012] (1) Identify or assist in identifying wheat stripe rust resistance;

[0013] (2) Wheat breeding;

[0014] (3) preparing products for identifying or assisting in identifying wheat stripe rust resistance;

[0015] (4) Prepare wheat breeding products.

[0016] The present invention also provides a method for identifying wheat stripe rust resistance, the method comprising: using the wheat genomic DNA to be tested as a template, performing PCR amplification using the SSR molecular marker primer set described above to obtain a PCR amplification product, performing electrophoresis detection on the PCR amplification product, and analyzing the wheat stripe rust resistance according to the electrophoresis detection result.

[0017] Herein, the PCR reaction system (10 μL) can be as follows: 10×PCR buffer 1.00 μL, dNTPs (10 mM) 0.25 μL, Taq enzyme (5 U / μL) 0.10 μL, upstream and downstream primers (1.0 μM) 2.00 μL, template DNA (25 ng / μL) 2.00 μL, and ddH2O 4.65 μL.

[0018] The PCR reaction used a touch down program as follows: (1) denaturation at 94°C for 5 min; (2) denaturation at 94°C for 30 s, annealing at 60°C for 30 s, and extension at 72°C for 30 s, with the annealing temperature decreasing by 0.5°C in each cycle, for 10 consecutive cycles; (3) denaturation at 94°C for 30 s, annealing at 55°C for 30 s, and extension at 72°C for 30 s, for 35 consecutive cycles; (4) extension at 72°C for 10 min, and the reaction was terminated.

[0019] In the above method, the analysis and determination of the wheat stripe rust resistance is as follows: if the PCR amplification product contains a band of the same size as the red wheat PCR product, the genotype of the wheat to be tested is AA; if the PCR amplification product contains a band of the same size as the Thatcher PCR product, the genotype of the wheat to be tested is BB; the stripe rust resistance of the wheat to be tested whose genotype of the SSR molecular marker is AA is higher than that of the wheat to be tested whose genotype of the SSR molecular marker is BB.

[0020] The band size that is the same as the PCR product of red wheat is 260 bp (nucleotide sequence is 5'-TTTCGGGCTGAACGTACAATCAACACAACACTATTCATGGATAACAGCACAGCACAACACAGGAGGATACCAAGAAAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGATAACACATATGAACACATCACAACACAACACTATTATTGATTGTTACATTCTTATTTATTTATTCTGAAAAGGAGAATAGAGCCCCGGCCACTGCATCTGAACGATGCATGCGTCCATTTTACTCCC-3' (SEQ ID No. 3).

[0021] The size of the band identical to that of the Thatcher PCR product is 252 bp (nucleotide sequence: 5′-TTTCGGGGCTGAACGTACAATCAACACAACACTATTCATGGATAACAGCACAGCACAACACAGGAGGATACCAAGAAAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGATAACACATATGAACACATCACAACACAACACTATTATTGATTGTTACATTCTTATTTATTTATTCTGAAAAGGAGAATAGAGCCCCGGCCACTGCATCTGAACGATGCATGCGTCCATTTTACTCCC-3′ (SEQ ID No. 4).

[0022] The present invention also provides a method for wheat breeding, which includes detecting the genotype of the SSR molecular marker mentioned above in the wheat genome, the genotype of the SSR molecular marker is AA or BB, and selecting wheat with the genotype AA of the SSR molecular marker as a parent for breeding, wherein the PCR product of the wheat with the genotype AA is the same size as the PCR product of red wheat; the wheat with the genotype BB is the same size as the Thatcher PCR product.

[0023] In the above breeding method, the substance for detecting the genotype of the SSR molecular marker mentioned above in the wheat genome is the SSR molecular marker primer set mentioned above.

[0024] In the above-mentioned applications, methods and products, the primer composition may be labeled or not labeled with a marker. The marker refers to any atom or molecule that can be used to provide a detectable effect and can be connected to a nucleic acid. Markers include but are not limited to dyes; radioactive labels, such as 32P; binding moieties, such as biotin; haptens, such as digoxin (DIG); luminescent, phosphorescent or fluorescent moieties; and fluorescent dyes alone or in combination with moieties that can inhibit or move the emission spectrum by fluorescence resonance energy transfer (FRET). The marker can provide a signal that can be detected by fluorescence, radioactivity, colorimetry, weight determination, X-ray diffraction or absorption, magnetism, enzyme activity, etc. The marker can be a charged moiety (positive or negative charge) or, alternatively, can be charge neutral. The marker can include a nucleic acid or protein sequence or a combination thereof, as long as the sequence containing the marker is detectable. In some embodiments, the nucleic acid is directly detected (e.g., directly read the sequence) without a marker.

[0025] The present invention also provides application of the above-mentioned method in wheat breeding.

[0026] The material for detecting the polymorphism and genotype of the SSR molecular marker site can be combined with other materials (such as materials for detecting single nucleotide polymorphism or genotype of other molecular markers related to wheat stripe rust resistance) to prepare a product for identifying wheat varieties with resistance to wheat stripe rust.

[0027] Herein, the purpose of the breeding may include cultivating wheat resistant to stripe rust. The wheat may be a pure line or an inbred line.

[0028] The wheat may be a recombinant inbred line population of Red Wheat, Thatcher or Thatcher×Red Wheat, wherein the Red Wheat is the male parent and the Thatcher is the female parent.

[0029] The present invention locates a stripe rust resistance QTL on the wheat chromosome 7AL, named QYr.cau-7AL, and simultaneously develops and obtains a SSR marker Xcau7AL23 that is closely linked to the chromosome, and is a co-dominant marker. The molecular marker provided by the present invention has the characteristics of rapidity, accuracy and high efficiency, and can be used to screen plants with the stripe rust resistance QTL QYr.cau-7AL, and uses molecular marker-assisted selection breeding to provide technical support for wheat stripe rust trait improvement and disease resistance breeding, and has important applications in wheat stripe rust resistance molecular marker-assisted breeding and resistance improvement. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 LOD curve and partial chromosome genetic map of the stripe rust resistance QTL (QYr.cau-7AL) located in the Thatcher×Hongqi wheat population.

[0031] Figure 2 The polyacrylamide gel electrophoresis diagram of the amplified products of the molecular marker Xcau7AL23 in the parents Hongqimai (male parent) and Thatcher (female parent) and some of the tested strains. The arrows in the figure indicate the linkage bands with QYr.cau-7AL in the disease-resistant parent Hongqimai and the tested strains. DETAILED DESCRIPTION

[0032] The present invention is further described in detail below in conjunction with specific embodiments, and the examples provided are only for illustrating the present invention, rather than for limiting the scope of the present invention. The examples provided below can be used as a guide for further improvements by those of ordinary skill in the art, and do not constitute a limitation of the present invention in any way.

[0033] The experimental methods in the following examples, unless otherwise specified, are all conventional methods, and are performed according to the techniques or conditions described in the literature in the field or according to the product instructions. The materials, reagents, etc. used in the following examples, unless otherwise specified, can all be obtained from commercial channels.

[0034] Unless otherwise specified, the quantitative tests in the following examples were performed three times and the results were averaged.

[0035] The wheat variety "Hongqimai" in the following embodiment is a local variety of wheat in my country, which has adult resistance to stripe rust, which has been recorded in: Wang Z., Ren J., Du Z., Che M., Zhang Y., Quan W., Jiang X., Ma Y., Zhao Y., and Zhang Z. Identification of a major QTL on chromosome arm 2AL for reducing yellow rust severity from a Chinese wheat landrace with evidence for durable resistance. Theoretical and Applied Genetics, 2019, 132, 457-471. The public can obtain the biological material from the applicant, which is only used for repeating the experiment of the present invention and cannot be used for other purposes.

[0036] The wheat variety Thatcher in the following examples is an American wheat variety with a pedigree of Marquis / Iumillo / / Marquis / Kanred, and is numbered CItr 10003 in the National Small Grain Collection (Aberdeen, Idaho 83210, USA), and is highly infected with stripe rust.

[0037] The wheat Mingxian 169 in the following examples has been recorded in: Wang Z., Ren J., Du Z., Che M., Zhang Y., Quan W., Jiang X., Ma Y., Zhao Y., and Zhang Z. Identification of a major QTL on chromosome arm 2AL for reducing yellow rust severity from a Chinese wheat landrace with evidence for durable resistance. Theoretical and Applied Genetics, 2019, 132, 457-471. The public can obtain the biological material from the applicant, and the biological material is only used for repeating the experiments of the present invention and cannot be used for other purposes.

[0038] The stripe rust fungus in the following embodiment is Puccinia striiformis CYR32 species, and the original strain was provided by Chen Wanquan and Xu Shichang, researchers at the Institute of Plant Protection, Chinese Academy of Agricultural Sciences, and has been recorded in: Wang Z., Ren J., Du Z., Che M., Zhang Y., Quan W., Jiang X., Ma Y., Zhao Y., and Zhang Z. Identification of a major QTL on chromosome arm 2AL for reducing yellowrust severity from a Chinese wheat landrace with evidence for durable resistance. Theoretical and Applied Genetics, 2019, 132, 457-471. The public can obtain the biological material from the applicant, and the biological material is only used for repeating the experiment of the present invention and cannot be used for other purposes.

[0039] The following examples used SAS 9.4 statistical software to process the data, and the experimental results were expressed as mean ± standard deviation. One-way ANOVA test was used, and P < 0.0001 (****) indicated a very significant difference.

[0040] Example 1. Location of wheat stripe rust resistance QTL (QYr.cau-7AL) and acquisition of closely linked markers

[0041] Samples to be tested: Thatcher×Hongqimai recombinant inbred line: Hongqimai is used as the male parent and Thatcher is used as the female parent. F1 seeds are obtained by hybridization. F2 seeds are harvested from plants grown from F1 seeds. In the following years, recombinant inbred lines are obtained by continuous self-pollination from generation to generation through the single-seed transmission method.

[0042] 1. Phenotypic identification of wheat stripe rust resistance

[0043] The recombinant inbred line population of Thatcher×Hongqimai and the male parent Hongqimai and the female parent Thatcher were planted in Wushan County, Tianshui City, Gansu Province and Shengzhuang Town, Tai'an City, Shandong Province, respectively. A randomized complete block design was adopted with three replications. The experimental plots consisted of a single row of 1 m in length with a row spacing of 25 cm. Each plot was sown with about 40 seeds of a recombinant inbred line, and a group of parents and susceptible controls (Hongqimai, Thatcher, Mingxian 169) were sown every 60 recombinant inbred line plots.

[0044] Southern Gansu is an important source of wheat stripe rust and a source of new subspecies in my country. Stripe rust oversummers on late-maturing spring wheat and volunteer seedlings. These wheat seedlings act as a "green bridge". After the stripe rust reproduces and accumulates, it provides a source of bacteria for the test wheat plants, achieving natural disease in the test area. In Tai'an, Shandong, the summer is hot and the stripe rust cannot oversummer. The test area needs to be artificially inoculated. When the plants are in the tillering to jointing period, a 0.04% Tween 20 aqueous solution is used to prepare a CYR32 summer spore suspension. After sunset, the spray is evenly sprayed on the leaf surface, and then the inoculated wheat plants are covered with plastic film to keep them moist overnight. The plastic film is uncovered before noon the next day. Repeat the inoculation 2 to 3 times within 10 days. The severity of the recombinant inbred line was investigated when the flag leaf severity of the diseased control Mingxian 169 plants reached 10-30%, 50-60%, and 80-90%, respectively.

[0045] Based on the three survey data, the area under the disease progression curve (AUDPC) of each recombinant inbred line was calculated according to the formula: AUDPC = Σ[(x i +x (i+1) ) / 2]×(t (i+1) -t i ), where x i and x (i+1) Respectively represent the ti and t (i+1) Date of medical investigation value, t (i+1) -t i Indicates the tth i and t (i+1) The number of days between dates.

[0046] 2. Genetic map construction and QTL analysis

[0047] DNA from Red Qimai, Thatcher and 126 recombinant inbred lines were extracted by CTAB method. The concentration of DNA stock solution was determined by NanoDrop2000 ultra-micro spectrophotometer, and then diluted to a working solution with a concentration of 50ng / μL. The DNA quality was detected by 0.8% agarose gel. SNP analysis was performed using Illumina iSelect 90K SNP array technology, which was completed by Beijing Capital Biotech Co., Ltd. (http: / / www.capitalbiotech.com), including allele clustering and genotype calling of SNP loci. Markers with missing values ​​greater than 10% and skewed segregation were removed, and redundant markers were removed using the BIN function in the QTL IciMapping V4.0 software. Then, linkage groups were constructed using JoinMap 4.0 software, and genetic distances were measured in centimorgans (cM) calculated by Kosambi function.

[0048] Based on the chromosome genetic map, genotype data and disease phenotype data, the composite interval mapping (CIM) method of Windows QTLCartographer 2.5 software was used for QTL analysis, and LOD = 2.5 was selected as the test standard. A stripe rust resistance QTL was located on the long arm of chromosome 7A ( Figure 1 ), named QYr.cau-7AL, which is within the 7.2cM interval defined by SNP markers IWB316 and IWB36301, and their physical positions in the IWGSC RefSeqv1.0 reference genome are 664.47Mb and 679.59Mb, respectively. SNP marker IWB36931 is located at the peak of the LOD value, that is, it is closely linked to QYr.cau-7AL, and its physical position in the reference genome is 670,780,961 to 670,781,061. QYr.cau-7AL can significantly reduce the severity of stripe rust, explaining 9% to 11% of the phenotypic variation of the disease, and the disease resistance locus comes from the wheat local variety Hongqimai.

[0049] Example 2: Development of SSR markers related to wheat stripe rust

[0050] Log in to the "Wheat Omics" online platform (http: / / wheatomics.sdau.edu.cn / ), use JBrowse to search for Predicted_SSR repeat motifs upstream and downstream of the IWB36931 site in the 7A chromosome sequence of the Chinese Spring reference genome (RefSeq v1.0), and use the PrimerServer tool to design SSR primers online according to their physical location. Then, PCR amplification was performed using the DNA of Hongqi wheat and Thatcher as templates to screen for polymorphic SSR markers. A total of 28 pairs of SSR primers were designed and synthesized in the 669.80-671.80Mb interval of chromosome 7AL, from which the marker Xcau7AL23 was screened out, which was polymorphic between the parents and had clear and easy-to-distinguish electrophoresis bands, and it was closely linked to IWB36931.

[0051] The SSR marker Xcau7AL23 consists of an upstream primer F and a downstream primer R. The specific nucleotide sequence information is as follows:

[0052] Upstream primer F: 5'-TTTCGGGGCTGAACGTACAA-3' (SEQ ID No. 1);

[0053] Downstream primer R: 5'-GGGAGTAAAATGGACGCATGC-3' (SEQ ID No. 2).

[0054] The molecular marker Xcau7AL23 is located at positions 671,733,466 to 671,733,723 of chromosome 7A of the wheat Chinese Spring reference genome (RefSeq v1.0). The molecular marker Xcau7AL23 has two genotypes. If the product size obtained by PCR using the upstream and downstream primers F / R is 260bp (i.e., the same type as the stripe rust-resistant wheat "Hongqimai", this gene type is defined as the AA genotype), the wheat to be tested is a stripe rust-resistant wheat variety; if the product size obtained by PCR using the upstream and downstream primers F / R is 252bp (i.e., the same type as the stripe rust-susceptible wheat Thatcher, this gene type is defined as the BB genotype), the wheat to be tested is a stripe rust-susceptible wheat variety.

[0055] Example 2: Application of molecular marker Xcau7AL23 in identifying wheat stripe rust resistance QYr.cau-7AL

[0056] 104 strains were randomly selected from the Thatcher×Hongqimai recombinant inbred line population for Xcau7AL23 marker detection. The specific method was as follows:

[0057] The genomic DNA of the 104 strains to be tested was extracted as a template, and PCR amplification was performed using the specific primer pair F / R (SEQ ID No. 1 and SEQ ID No. 2) of the molecular marker Xcau7AL23 as primers, and electrophoresis detection was performed.

[0058] The PCR reaction system (10 μL) is as follows: 10×PCR buffer 1.00 μL, dNTPs (10 mM)

[0059] 0.25μL, Taq enzyme (5U / μL) 0.10μL, upstream and downstream primers (1.0μM) 2.00μL, template DNA (25ng / μL) 2.00μL, ddH2O 4.65μL.

[0060] The PCR reaction used the touch down program as follows: (1) denaturation at 94°C for 5 min; (2) denaturation at 94°C for 30 s, annealing at 60°C for 30 s, and extension at 72°C for 30 s, with the annealing temperature decreasing by 0.5°C in each cycle, for 10 cycles; (3) denaturation at 94°C for 30 s, annealing at 55°C for 30 s, and extension at 72°C for 30 s, for 35 cycles; (4) extension at 72°C for 10 min, and the reaction was terminated.

[0061] The PCR amplification products were detected by 6% polyacrylamide denaturing gel electrophoresis, with 1×TBE buffer, 60W constant power, 3000V voltage, and 50min electrophoresis time, followed by silver staining.

[0062] The results are as follows Figure 2 As shown: If the PCR product contains a band of the same size as the red wheat PCR product, which is 260bp, the genotype of the wheat to be tested is "AA"; if the PCR product contains a band of the same size as the Thatcher PCR product, which is 252bp, the genotype of the wheat to be tested is "BB".

[0063] The genotypes of each line and the AUDPC values ​​of the severity of stripe rust in the field in Gansu are shown in Table 1. Among the SSR marker Xcau7AL23, there were 53 plants of the same type as "Hongqimai" containing the stripe rust resistance QTL QYr.cau-7AL (i.e., genotype AA), and 51 plants of the same type as "Thatcher" without QYr.cau-7AL (i.e., genotype BB). Statistical tests (Table 2) showed that the average stripe rust AUDPC value of the former (349.20±164.79) was significantly lower than that of the latter (650.35±157.16), indicating that the genetic effect of QYr.cau-7AL reached an extremely significant level (P<0.0001).

[0064] Table 1. Correspondence between Xcau7AL23 genotype and stripe rust phenotype of Thatcher×Hongqimai recombinant inbred line

[0065]

[0066]

[0067]

[0068]

[0069]

[0070] Note: AA in the table means that the sample has the same genotype as Red Thatcher; BB means that the sample has the same genotype as Thatcher and Red Thatcher.

[0071] Table 2. Effect of QYr.cau-7AL on stripe rust resistance in 104 recombinant inbred lines of Thatcher×Hongqimai

[0072]

[0073] The above results show that the wheat materials with AA genotype at the Xcau7AL23 locus in the wheat genome have higher or potential higher resistance to stripe rust than the wheat with BB genotype at the Xcau7AL23 locus in the wheat genome. It can be seen that the use of SSR marker Xcau7AL23 can effectively screen wheat lines containing QYr.cau-7AL, improve selection efficiency, and be used for disease resistance breeding of wheat.

[0074] The present invention has been described in detail above. For those skilled in the art, without departing from the purpose and scope of the present invention, and without the need to carry out unnecessary experimental conditions, the present invention can be implemented in a wide range under equivalent parameters, concentrations and conditions. Although the present invention provides specific embodiments, it should be understood that the present invention can be further improved. In a word, according to the principles of the present invention, the application is intended to include any changes, uses or improvements to the present invention, including departure from the disclosed scope in the application, and changes made with conventional techniques known in the art.

Claims

1. A wheat SSR molecular marker primer set, characterized in that: The SSR molecular marker primer set consists of primer F and primer R, wherein primer F is a DNA molecule with a nucleotide sequence such as SEQ ID No.1, and primer R is a DNA molecule with a nucleotide sequence such as SEQ ID No.

2.

2. A kit, characterized in that: The kit comprises the SSR molecular marker primer set according to claim 1.

3. A DNA chip, characterized in that The DNA chip comprises the SSR molecular marker primer set according to claim 1.

4. Any of the following uses of the SSR molecular marker primer set of claim 1, and / or the kit of claim 2, and / or the DNA chip of claim 3: (1) Identify or assist in identifying wheat stripe rust resistance; (2) Wheat breeding; (3) preparing products for identifying or assisting in identifying wheat stripe rust resistance; (4) Prepare wheat breeding products.

5. A method for identifying wheat stripe rust resistance, characterized in that: The method comprises: using the wheat genomic DNA to be tested as a template, performing PCR amplification using the SSR molecular marker primer set of claim 1 to obtain a PCR amplification product, performing electrophoresis detection on the PCR amplification product, and analyzing the wheat stripe rust resistance ability based on the electrophoresis detection result.

6. The method according to claim 5, characterized in that The analysis and determination of the wheat stripe rust resistance is as follows: if the PCR amplification product contains a band of the same size as the red wheat PCR product, which is 260bp, then the genotype of the wheat to be tested is AA; if the PCR amplification product contains a band of the same size as the Thatcher PCR product, which is 252bp, then the genotype of the wheat to be tested is BB; the stripe rust resistance of the wheat to be tested whose genotype of the SSR molecular marker is AA is higher than that of the wheat to be tested whose genotype of the SSR molecular marker is BB.

7. A method for wheat breeding, characterized in that: The method comprises detecting the genotype of the SSR molecular marker described in claim 1 in the wheat genome, wherein the genotype of the SSR molecular marker is AA or BB, and selecting wheat with the genotype AA of the SSR molecular marker as a parent for breeding, wherein the PCR product of the wheat with the genotype AA is the same size as the PCR product of red wheat; and the wheat with the genotype BB is the same size as the PCR product of Thatcher.

8. The method according to claim 7, characterized in that: The material of the genotype of the SSR molecular marker is the primer set according to claim 1.

9. Use of the method according to any one of claims 5 to 8 in wheat breeding.

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