Forestry waste treatment method for improving forest carbon sequestration capacity

Abstract

The invention discloses a forestry waste treatment method for improving the carbon sequestration capacity of forests, and belongs to the technical field of forestry waste treatment.The forestry waste treatment method includes the steps that forestry waste is subjected to composting and then returned to forests, and the method specifically comprises the steps of material smashing, carbon nitrogen ratio adjusting, water content and pH adjusting, composting, decomposing and the like; an enzyme activity regulating agent is prepared and mixed into material residues for composting, the prepared enzyme activity regulating agent absorbs high-concentration glucose generated by decomposition in a high-temperature stage so as to reduce the inhibition effect of the high-concentration glucose on cellulase, meanwhile, the regulating agent further improves the activity of the cellulase, and by matching with control over conditions such as moisture and pH, the content of the cellulase is improved. According to the present invention, the efficient composting is ensured, the composting decomposition rate and the composting decomposition degree are improved, the fertilizer obtained through the composting can effectively improve the physical and chemical properties of the soil in the forest, can promote the forest growth, and can improve the forest stand quality and the carbon sequestration ability, and the forestry waste treatment method has important significance on the resource utilization of the forestry waste and the forest carbon sequestration and sink increase.

Description

Technical Field

[0001] The invention relates to the technical field of forestry waste treatment, and in particular to a forestry waste treatment method for improving the carbon sequestration capacity of forests. Background Art

[0002] With the continuous expansion of forestry production, the amount of forestry waste has increased year by year. The traditional landfill and incineration treatment methods will not only cause a huge waste of resources, but also cause serious pollution to the environment. At the same time, forestry waste has the characteristics of large quantity, many types and wide distribution. Therefore, effectively utilizing forestry waste resources and maximizing their value is of great significance for saving energy, reducing carbon emissions, protecting the environment and promoting production.

[0003] Composting technology is a process in which forestry waste is crushed and then decomposed and fermented by microorganisms such as fungi, bacteria and actinomycetes to finally form stable humus. Composting is currently a forestry waste treatment technology with a high resource utilization rate. Returning forestry waste compost products to the forest can reduce the use of chemical fertilizers, improve the carbon sequestration capacity of soil in the forest, and at the same time improve the physical and chemical properties of the soil to promote good growth of trees, increase forest biomass and carbon storage, and thus improve the carbon sequestration capacity of the forest. Therefore, returning forestry waste to the forest through composting is an important measure to increase the carbon sink capacity of the forest.

[0004] Due to the large quantity and high weight of forestry waste, transporting it outside the forest to a specific site for composting and then transporting it back to the forest for application will greatly increase transportation and operation costs. Therefore, it is a more rational method to compost in situ in the forest and directly apply the compost to the forest to improve the quality of the forest stand. However, when composting directly in situ in the forest, the maintenance operations such as turning the compost are more complicated. In addition, compared with other composting raw materials, forestry waste contains more difficult-to-decompose organic matter such as cellulose and hemicellulose, and its natural decomposition is extremely slow. Under conventional methods, the natural composting decomposition rate is relatively long. If it is not handled properly, the decomposition is incomplete and stable humus is not formed, which will have adverse effects during the application process.

[0005] Therefore, finding an in-situ composting method suitable for forestry waste to reduce management costs while increasing the composting rate and maturity of forestry waste is an urgent problem that needs to be solved. Summary of the invention

[0006] In view of this, the purpose of the present invention is to provide a method for treating forestry waste for improving the carbon sequestration capacity of forests, so as to solve the problems of high management cost, slow composting rate and incomplete decomposition when carrying out in-situ composting of forestry waste under conventional methods.

[0007] The present invention solves the above technical problems by the following technical means:

[0008] A method for treating forestry waste to improve the carbon sequestration capacity of forests, the method being as follows:

[0009] (1) Material crushing: Collect forest waste branches and crush them into particles with a diameter of 0.5 to 5 cm to obtain fine slag;

[0010] (2) Carbon-nitrogen ratio adjustment: adding nitrogen source to the refined slag to adjust the carbon-nitrogen ratio;

[0011] (3) Water content and pH adjustment: The water content and pH of the refined slag are adjusted to obtain pretreated slag, and then the composite microbial solution is added to mix thoroughly, and then the enzyme activity regulator is added to mix to obtain a bulk material;

[0012] (4) Pile making and composting: The materials are piled up and then composted to obtain compost and then returned to the forest.

[0013] Furthermore, in step (2), the nitrogen source is any one of cow dung, pig dung, chicken dung, seaweed powder and urea.

[0014] Furthermore, in the step (2), the carbon-nitrogen ratio is adjusted to (25-30):1.

[0015] Furthermore, in step (3), the water content is adjusted to 65-75%, and the pH is adjusted to 7-8.

[0016] Furthermore, in step (3), the composite microbial solution is obtained by mixing Bacillus subtilis solution, Bacillus licheniformis solution and thermoactinomycete solution in a volume ratio of 2:1:1, and the concentration of the composite microbial solution is 1 to 3×10 9 cfu / ml.

[0017] Furthermore, the enzyme activity regulator comprises the following raw materials:

[0018] Polyethylene glycol, calcium pyrophosphate, 2wt% hydrochloric acid solution, 3-aminophenylboric acid, ammonium persulfate, polyethylene glycol diether, curdlan, p-tert-butylaniline, graphene, and sodium hydroxide solution with pH=12.

[0019] Further, the preparation method of the enzyme activity regulator is as follows:

[0020] A: Dissolve polyethylene glycol in water to prepare a 2-5wt% polyethylene glycol solution and put it into a reactor. Add calcium pyrophosphate into a 2wt% hydrochloric acid solution, stir to dissolve, and then add it into the reactor. Heat to 60-80°C for reaction for 20-40min. Cool to room temperature and adjust the pH to 6-7. Add 3-aminophenylboric acid and stir to mix evenly. Then add ammonium persulfate and polyethylene glycol diether. Heat to 55-65°C for reaction for 2-4h. Cool to room temperature and dry to a water content of 10-20%. Then put it into a granulator to prepare granules with a particle size of 2-8mm.

[0021] B: Add curdlan to a sodium hydroxide solution with a pH of 12, stir to dissolve, then add p-tert-butylaniline, stir to react at room temperature for 30 to 60 minutes, then add graphene and stir to mix evenly, heat to 55 to 65°C, continue stirring to react for 5 to 10 minutes, spray it onto the surface of the colloidal particles while hot, and let it stand overnight at room temperature to obtain the enzyme activity regulator.

[0022] Forestry waste contains a large amount of cellulose and other difficult-to-degrade organic substances. When composting, the temperature of the pile gradually rises and enters the high-temperature stage. At this time, microorganisms are highly active and secrete cellulase to decompose cellulose to produce glucose. When cellulose gradually decomposes to produce a large amount of glucose, the high concentration of glucose will inhibit the activity of cellulase, especially β-glucosidase, and affect the decomposition of the remaining cellulose, thereby extending the composting time and reducing the degree of maturity. Therefore, the present invention prepares an enzyme activity regulator and adds it to the pile for composting. Specifically, the enzyme activity regulator is made of polyethylene glycol gel loaded with 3-aminophenylboronic acid to form a granule, and then sprayed with curdlan to encapsulate it. When the compost enters the high-temperature stage, the surface-coated curdlan is reduced to a liquid state and peeled off from the surface of the colloidal particles, exposing the internal colloidal particles. The 3-aminophenylboronic acid loaded in the colloidal particles adsorbs and binds to glucose molecules, reducing the glucose concentration in the surrounding pile, thereby reducing the inhibitory effect of high-concentration glucose on the activity of cellulase. Furthermore, the calcium pyrophosphate in the colloidal particles enters the pile in the form of ions to further activate the activity of cellulase. Thus, the enzyme activity regulator improves the decomposition of cellulose by microorganisms by reducing the inhibitory effect of high-concentration glucose and activating enzyme activity, thereby accelerating the reaction rate and maturity of the compost.

[0023] However, while the 3-aminophenylboronic acid loaded on the polyethylene glycol colloids in the enzyme activity regulator adsorbs glucose molecules, the polyethylene glycol itself has multiple active sites for adsorbing amino acids, inorganic salt ions, etc. in the pile, and the added calcium pyrophosphate can shield and cover these active sites, thereby reducing the nonspecific adsorption of the polyethylene glycol colloids to inorganic salt ions and other substances, ensuring that the colloids can better play the role of adsorbing glucose molecules.

[0024] Since the gel formed by curdlan needs to reach a temperature of about 60°C before it can be reduced from a gel state to a liquid state, in order to ensure that the wrapped curdlan can be separated from the colloid particles in time to ensure that the colloid particles can effectively play their role, the curdlan is treated with tert-butylaniline to reduce its liquid transition temperature by reducing the degree of aggregation between molecules. At the same time, graphene particles are embedded in the curdlan colloid. Graphene can absorb the surrounding heat to further promote the timely shedding of curdlan. At the same time, graphene has high thermal conductivity to transfer heat in the pile, thereby ensuring the consistency of temperature in the pile without turning the pile to promote uniform decomposition of the pile.

[0025] Furthermore, in step A, the mass ratio of the polyethylene glycol solution, calcium pyrophosphate, 2 wt% hydrochloric acid solution, 3-aminophenylboric acid, ammonium persulfate, and polyethylene glycol diether is (3-6): (0.2-0.6): (1-3): (0.5-1): (0.01-0.02): (0.03-0.06).

[0026] Furthermore, in the step B, the mass ratio of the curd rubber, the sodium hydroxide solution, the p-tert-butylaniline and the graphene is (1-2): (3-6): (0.1-0.2): (0.04-0.08).

[0027] Furthermore, the mass ratio of the pretreated residue to the enzyme activity regulator is 100:(4-7).

[0028] Beneficial effects:

[0029] The present invention prepares an enzyme activity regulator during in-situ composting of forestry waste for mixed composting, and the enzyme activity regulator adsorbs the high-concentration glucose generated by the compost at a high temperature stage to reduce the inhibitory effect of high-concentration glucose on the activity of cellulase, and can further activate cellulase, so that the cellulase can further decompose cellulose in the material, shorten the decomposition time, improve the degree of decomposition, and reduce the cost of composting management. The fertilizer formed by composting can effectively improve the physical and chemical properties of soil in the forest, thereby promoting better growth of trees and improving the quality of forest stands. DETAILED DESCRIPTION

[0030] The present invention will be described in detail below with reference to specific embodiments:

[0031] The invention discloses a method for treating forestry waste for improving the carbon fixation capacity of forests. Before composting the forestry waste, an enzyme activity regulator is first prepared, as shown below.

[0032] Example 1: Preparation of enzyme activity regulator

[0033] A: Dissolve polyethylene glycol in water to prepare 4 kg of 3 wt% polyethylene glycol solution and put it into a reactor, put 0.4 kg of calcium pyrophosphate into 2 kg of 2 wt% hydrochloric acid solution, stir and dissolve, then add it into the reactor, heat to 70°C for reaction for 30 min, cool to room temperature and adjust pH to 6.5, add 0.7 kg of 3-aminophenylboric acid and stir to mix evenly, then add 0.015 kg of ammonium persulfate and 0.04 kg of polyethylene glycol diether, heat to 60°C for reaction for 3 h, cool to room temperature and dry at 45°C until the moisture content is 15%, then put it into a granulator to make granules with a particle size of 4 mm;

[0034] B: Add 1.5 kg of curdlan to 4.5 kg of sodium hydroxide solution with a pH of 12, stir to dissolve, then add 0.5 kg of p-tert-butylaniline, stir to react at room temperature for 40 minutes, then add 0.05 kg of graphene and stir to mix evenly, heat to 60°C and continue stirring to react for 7 minutes, spray evenly onto the surface of the gel particles while hot, with a spraying thickness of about 1.5 mm, and let stand overnight at room temperature to obtain the enzyme activity regulator.

[0035] Example 2: Preparation of enzyme activity regulator II

[0036] A: Dissolve polyethylene glycol in water to prepare 3 kg of 2 wt% polyethylene glycol solution and put it into a reactor, add 0.2 kg of calcium pyrophosphate to 1 kg of 2 wt% hydrochloric acid solution, stir and dissolve, then add it into the reactor, heat to 60°C for reaction for 20 min, cool to room temperature and adjust pH to 6, add 0.5 kg of 3-aminophenylboric acid and stir and mix well, then add 0.01 kg of ammonium persulfate and 0.03 kg of polyethylene glycol diether, heat to 55°C for reaction for 2 h, cool to room temperature and dry at 45°C until the moisture content is 10%, then put it into a granulator to make granules with a particle size of 2 mm;

[0037] B: Add 1 kg of curdlan to 3 kg of sodium hydroxide solution with a pH of 12, stir to dissolve, then add 0.1 kg of p-tert-butylaniline, stir to react at room temperature for 30 minutes, then add 0.04 kg of graphene and stir to mix evenly, heat to 55°C and continue stirring to react for 5 minutes, spray evenly onto the surface of the gel particles while hot, with a spraying thickness of about 1 mm, and let stand overnight at room temperature to obtain the enzyme activity regulator.

[0038] Example 3: Preparation of enzyme activity regulator

[0039] A: Dissolve polyethylene glycol in water to prepare 6 kg of 4 wt% polyethylene glycol solution and put it into a reactor, add 0.6 kg of calcium pyrophosphate into 3 kg of 2 wt% hydrochloric acid solution, stir and dissolve, then add it into the reactor, heat to 80°C for reaction for 40 min, cool to room temperature and adjust pH to 7, add 1 kg of 3-aminophenylboric acid and stir and mix well, then add 0.02 kg of ammonium persulfate and 0.06 kg of polyethylene glycol diether, heat to 65°C for reaction for 4 h, cool to room temperature and dry at 45°C until the moisture content is about 18%, then put it into a granulator to make granules with a particle size of 6 mm;

[0040] B: Add 2 kg of curdlan to 6 kg of sodium hydroxide solution with a pH of 12, stir to dissolve, then add 0.2 kg of p-tert-butylaniline, stir to react at room temperature for 60 min, then add 0.08 kg of graphene and stir to mix evenly, heat to 65°C and continue stirring to react for 10 min, spray evenly onto the surface of the gel particles while hot, with a spraying thickness of about 2 mm, and let stand overnight at room temperature to obtain the enzyme activity regulator.

[0041] Comparative Example 1: Preparation of enzyme activity regulator

[0042] In contrast to Example 1, the only difference is that calcium pyrophosphate is not added in step A when preparing the enzyme activity regulator in Comparative Example 1, as follows:

[0043] A: Dissolve polyethylene glycol in water to prepare 4 kg of 3 wt% polyethylene glycol solution and put it into a reactor, adjust the pH to 6.5, add 0.7 kg of 3-aminophenylboric acid and stir to mix evenly, then add 0.015 kg of ammonium persulfate and 0.04 kg of polyethylene glycol diether, heat to 60 ° C and stir to react for 3 hours, cool to room temperature and dry at 45 ° C to a moisture content of 15%, and then put it into a granulator to make granules with a particle size of 4 mm;

[0044] B: Same as Example 1

[0045] Comparative Example 2: Preparation of enzyme activity regulator

[0046] In contrast to Example 1, the only difference is that in the preparation of the enzyme activity regulator in Comparative Example 2, p-tert-butylaniline is not added in step B, as follows:

[0047] A: Same as Example 1;

[0048] B: Add 1.5 kg of curdlan to 4.5 kg of sodium hydroxide solution with a pH of 12, stir to dissolve, then add 0.05 kg of graphene and stir to mix evenly, heat to 60°C and stir to react for 7 minutes, spray evenly onto the surface of the colloidal particles while hot, with a spraying thickness of about 1.5 mm, and let stand overnight at room temperature to obtain the enzyme activity regulator.

[0049] Comparative Example 3: Preparation of enzyme activity regulator

[0050] In contrast to Example 1, the only difference is that the preparation of the enzyme activity regulator in Comparative Example 3 lacks step B, which is as follows:

[0051] A: Dissolve polyethylene glycol in water to prepare 4 kg of 3 wt% polyethylene glycol solution and put it into a reactor, put 0.4 kg of calcium pyrophosphate into 2 kg of 2 wt% hydrochloric acid solution, stir and dissolve, then add it into the reactor, heat to 70°C for reaction for 30 min, cool to room temperature and adjust the pH to 6.5, add 0.7 kg of 3-aminophenylboric acid and stir to mix evenly, then add 0.015 kg of ammonium persulfate and 0.04 kg of polyethylene glycol diether, heat to 60°C for reaction for 3 h, cool to room temperature and dry at 45°C to a moisture content of 15%, then put it into a granulator to make granules with a particle size of 4 mm, which is an enzyme activity regulator.

[0052] Comparative Example 4: Preparation of enzyme activity regulator

[0053] In contrast to Example 1, the only difference is that the amount of calcium pyrophosphate added during the preparation of the enzyme activity regulator in Comparative Example 4 is 0.05 kg, and the remaining steps are the same as in Example 1.

[0054] Comparative Example 5: Preparation of enzyme activity regulator

[0055] In contrast to Example 1, the only difference is that the amount of calcium pyrophosphate added during the preparation of the enzyme activity regulator in Comparative Example 5 is 0.8 kg, and the remaining steps are the same as in Example 1.

[0056] Comparative Example 6: Preparation of enzyme activity regulator

[0057] In contrast to Example 1, the only difference is that the enzyme activity regulator in Comparative Example 6 is prepared according to a conventional method, as follows:

[0058] A: Dissolve polyethylene glycol in water to prepare 4 kg of 3 wt% polyethylene glycol solution and put it into a reactor, adjust the pH to 6.5, add 0.7 kg of 3-aminophenylboric acid and stir to mix evenly, then add 0.015 kg of ammonium persulfate and 0.04 kg of polyethylene glycol diether, heat to 60 ° C and stir to react for 3 hours, cool to room temperature and dry at 45 ° C to a moisture content of 15%, then put it into a granulator to make 4 mm granules, which are the regulating agent.

[0059] Comparative Example 7: Preparation of enzyme activity regulator

[0060] In contrast to Example 1, the only difference is that the polyethylene glycol granules are replaced with activated carbon when preparing the enzyme activity regulator in Comparative Example 1, as follows:

[0061] The activated carbon is granulated into particles with a particle size of about 4 mm; 1.5 kg of curdlan is added to 4.5 kg of sodium hydroxide solution with a pH of 12, and 0.5 kg of p-tert-butylaniline is added after stirring to dissolve, and 0.05 kg of graphene is added after stirring and reacting at room temperature for 40 minutes, and the mixture is stirred and mixed evenly, and the temperature is raised to 60° C. and the stirring and reaction is continued for 7 minutes, and the activated carbon is evenly sprayed onto the surface of the activated carbon particles while hot, with a spraying thickness of about 1.5 mm. The enzyme activity regulator is obtained after standing at room temperature overnight.

[0062] Example 4: Method for treating forest waste to improve forest carbon sequestration capacity

[0063] The composite microbial culture liquid used in this example has a concentration of about 2×10 9 The Bacillus subtilis bacterial solution, the thermoactinomycete bacterial solution and the Bacillus licheniformis bacterial solution are mixed in a volume ratio of 2:1:1.

[0064] (1) Material crushing: collecting forestry waste branches and crushing them in situ to a particle size of about 3 cm to obtain fine slag;

[0065] (2) Adjustment of carbon-nitrogen ratio: Add chicken manure to the refined residue to adjust the carbon-nitrogen ratio to 26:1;

[0066] (3) Water content and pH adjustment: The water content of the refined slag is adjusted to 70%, and the pH is adjusted to 7.5 to obtain a pretreated slag; the pretreated slag and the composite microbial liquid are mixed at a mass ratio of 100:1, and then the enzyme activity regulator prepared by the method of Example 1 is added at a mass ratio of pretreated slag: enzyme activity regulator = 100:5, and mixed to obtain a bulk material;

[0067] (4) Pile making and composting: The materials are piled into a trapezoidal pile with a height of 2 m, a width of 1.5 m and a length of 1.5 m for composting.

[0068] Experiment 1: Experiment on forestry waste treatment methods to improve forest carbon sequestration capacity

[0069] 1. For comparison, the enzyme activity regulators prepared in Example 1 and Comparative Examples 1 to 7 were subjected to a forestry waste composting experiment. The experiment was specifically conducted in a fir forest at the base of Jinshan Village, Shibao Town, Zhong County, Chongqing. The experiment was divided into 9 groups: experimental group 1, control groups 1 to 7, and a blank control group. The enzyme activity regulators and composting methods used in each group are as follows:

[0070] Experimental group 1: the enzyme activity regulator prepared by the method of Example 1 and the composting method of Example 4;

[0071] Control groups 1 to 7: using the enzyme activity regulators prepared in Comparative Examples 1 to 7 and the composting method of Example 4 respectively;

[0072] Blank control group: no enzyme activity regulator was used, and the remaining steps were the same as those in Example 4.

[0073] 2. Record the time of completion of composting in each group (the composting is considered complete when the temperature in the center of the pile drops to room temperature, no irritating or unpleasant odor is emitted, and the pH is stable in the range of 6.5 to 7.5). After the composting is completed, measure the cellulose degradation rate of each group, and the data are shown in Table 1:

[0074] Composting time (d) Cellulose degradation rate (%) Experimental Group 1 36 49.67 Control group 1 38 42.55 Control group 2 37 44.29 Control group 3 40 46.13 Control group 4 37 45.96 Control group 5 37 47.52 Control group 6 41 37.47 Control group 7 40 41.94 Blank control 42 28.34

[0075] According to the data analysis in Table 1:

[0076] (1) Experimental group 1 was added with the enzyme activity regulator prepared by the method of the present invention, which could more efficiently degrade cellulose in forestry waste and shorten the composting time. Specifically, the composting time was shortened to 36 days. After the composting was completed, the cellulose degradation rate could reach 49.67%.

[0077] (2) The enzyme activity regulator in control group 1 failed to effectively activate the cellulase activity because calcium pyrophosphate was not added. At the same time, more active sites were exposed in the sodium alginate gel to adsorb components other than glucose molecules, including microorganisms, thereby reducing the amount of glucose molecules adsorbed. In turn, the activity of microorganisms and their secreted enzymes was inhibited, which prolonged the composting time and significantly reduced the cellulose degradation rate.

[0078] (3) In the control group 2, since the enzyme activity regulator did not contain tert-butylaniline, the coated curdlan was unable to be separated from the colloid particles in a timely and efficient manner when entering the high-temperature maturity stage, which limited the function of the internal colloid particles. In the control group 3, since the enzyme activity regulator did not use curdlan for embedding, the colloid particles adsorbed low-concentration glucose molecules at the beginning of composting, which resulted in less carbon source available to the microorganisms in the early stage of composting, reduced the physiological metabolic activity of the microorganisms in the early stage of composting, and prolonged the composting time.

[0079] (4) In the control group 7, the colloid particles were replaced with activated carbon particles. The activated carbon indiscriminately adsorbed glucose molecules and substances including cellulase, reducing the concentration of active enzymes. The enzyme activity was greatly inhibited and the regulatory effect on composting was small. As a result, the composting time was long and the cellulose decomposition rate was low.

[0080] Experiment 2: Forestry waste restoration experiment

[0081] The fertilizer obtained by composting the experimental group 1, control groups 1 to 7, and the blank control group in Experiment 1 was subjected to an effect test experiment. Specifically, soil was dug from the fir forest in Jinshan Village, Shibao Town, Zhong County, and divided into pots up to 2 / 3 of the pots. Then, 40 one-year-old Phoebe zhennan seedlings with consistent growth conditions were selected and planted in pots filled with forest soil. Then, they were evenly divided into 9 groups: corresponding to the experimental group 1, control groups 1 to 7, and the blank control group, with 5 pots in each group. After 20 days, the fertilizer obtained by composting in Experiment 1 was applied to each group, as follows:

[0082] Experimental group 1: 1 kg of fertilizer obtained from experimental group 1 in experiment 1 was applied;

[0083] Control groups 1 to 7: 1 kg of fertilizer obtained from control groups 1 to 7 in experiment 1 was applied respectively;

[0084] Blank control group: Apply 1 kg of fertilizer obtained from the blank control group in Experiment 1.

[0085] The same management method was used for maintenance, and then the plant height growth of each group of Phoebe zhennan was measured after 4 months and 8 months of fertilizer application. The data obtained are shown in Table 2:

[0086] Table 2

[0087]

[0088]

[0089] According to the data analysis in Table 2:

[0090] The fertilizer obtained by composting forestry waste can provide nutrients required for tree growth, promote tree growth, and improve forest quality. According to the plant height growth data of each group of experimental group 1, control groups 1-7, and blank control group, the fertilizer obtained by composting forestry waste using the method of the present invention is more completely decomposed, has more sufficient nutrients, and has a better effect on promoting tree growth. Specifically, after the fertilizer obtained by composting experimental group 1 in this experiment 1 is applied to Phoebe zhennan, the plant height growth of Phoebe zhennan in the 4th and 8th months is better than that of control groups 1-7 and blank control group.

[0091] The above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the present invention. Although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the purpose and scope of the technical solutions of the present invention, which should be included in the scope of the claims of the present invention. The techniques, shapes, and structural parts not described in detail in the present invention are all known technologies.

Claims

1. A method for treating forestry waste to improve the carbon sequestration capacity of forests, characterized in that: The method is as follows: (1) Material crushing: collecting forestry waste branches and crushing them into particles with a diameter of 0.5 to 5 cm to obtain fine slag; (2) Carbon-nitrogen ratio adjustment: adding nitrogen source to the refined slag to adjust the carbon-nitrogen ratio; (3) Water content and pH adjustment: The water content and pH of the refined slag are adjusted to obtain pretreated slag, and then the composite microbial solution is added to mix thoroughly, and then the enzyme activity regulator is added to mix to obtain a bulk material; (4) Pile making and composting: The materials are piled up and then composted to obtain compost and then returned to the forest.

2. A method for treating forestry waste to improve the carbon sequestration capacity of forests according to claim 1, characterized in that: In the step (2), the nitrogen source is any one of cow dung, pig dung, chicken dung, seaweed powder and urea.

3. A method for treating forestry waste to improve the carbon sequestration capacity of forests according to claim 2, characterized in that: In the step (2), the carbon-nitrogen ratio is adjusted to (25-30):

1.

4. A method for treating forestry waste to improve the carbon sequestration capacity of forests according to claim 3, characterized in that: In the step (3), the water content is adjusted to 65-75%, and the pH is adjusted to 7-8.

5. A method for treating forestry waste to improve the carbon sequestration capacity of forests according to claim 4, characterized in that: The composite microbial solution in step (3) is obtained by mixing Bacillus subtilis solution, Bacillus licheniformis solution and thermoactinomycete solution in a volume ratio of 2:1:1, and the concentration of the composite microbial solution is 1 to 3×10 9 cfu / mL.

6. A method for treating forestry waste to improve the carbon sequestration capacity of forests according to claim 5, characterized in that: The enzyme activity regulator comprises the following raw materials: Polyethylene glycol, calcium pyrophosphate, 2wt% hydrochloric acid solution, 3-aminophenylboric acid, ammonium persulfate, polyethylene glycol diether, curdlan, p-tert-butylaniline, graphene, and sodium hydroxide solution with pH=12.

7. A method for treating forestry waste to improve the carbon sequestration capacity of forests according to claim 6, characterized in that: The preparation method of the enzyme activity regulator is as follows: A: Dissolve polyethylene glycol in water to prepare a 2-5wt% polyethylene glycol solution and put it into a reactor. Add calcium pyrophosphate into a 2wt% hydrochloric acid solution, stir to dissolve, and then add it into the reactor. Heat to 60-80°C for reaction for 20-40min. Cool to room temperature and adjust the pH to 6-7. Add 3-aminophenylboric acid and stir to mix evenly. Then add ammonium persulfate and polyethylene glycol diether. Heat to 55-65°C for reaction for 2-4h. Cool to room temperature and dry to a water content of 10-20%. Then put it into a granulator to prepare granules with a particle size of 2-8mm. B: Add curdlan to a sodium hydroxide solution with a pH of 12, stir to dissolve, then add p-tert-butylaniline, stir to react at room temperature for 30 to 60 minutes, then add graphene and stir to mix evenly, heat to 55 to 65°C, continue stirring to react for 5 to 10 minutes, spray it onto the surface of the colloidal particles while hot, and let it stand overnight at room temperature to obtain the enzyme activity regulator.

8. A method for treating forestry waste to improve the carbon sequestration capacity of forests according to claim 7, characterized in that: In the step A, the mass ratio of the polyethylene glycol solution, calcium pyrophosphate, 2 wt% hydrochloric acid solution, 3-aminophenylboric acid, ammonium persulfate, and polyethylene glycol diether is (3-6): (0.2-0.6): (1-3): (0.5-1): (0.01-0.02): (0.03-0.06).

9. A method for treating forestry waste to improve the carbon sequestration capacity of forests according to claim 8, characterized in that: In the step B, the mass ratio of the curd rubber, the sodium hydroxide solution, the p-tert-butylaniline and the graphene is (1-2): (3-6): (0.1-0.2): (0.04-0.08).

10. The method for treating forestry waste to improve the carbon sequestration capacity of forests according to claim 1, characterized in that: The mass ratio of the pretreated residue to the enzyme activity regulator is 100:(4-7).

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