Dual-protein preparation and application thereof in hair care products

Abstract

The invention provides a dual-protein preparation and application thereof in hair care products. A preparation method of the dual-protein preparation comprises the following steps: A, extracting collagen; b, extracting keratin; and C, mixing the collagen and the keratin to prepare the dual-protein preparation. A collagen extraction process in the step A comprises the following steps: a, firstly, cleaning and airing cod skin; b, the treated cod skin is added into a shearing machine to be sheared into fragments, the fragments are put into a stainless steel barrel, enzymatic hydrolysate is added for enzymolysis, and enzymolysis mixed slurry is obtained after uniform stirring; c, adding the enzymolysis mixed slurry into an ultrasonic machine, and carrying out pneumatic ultrasonic extraction to release water-based protein so as to form cod skin active mixed liquid; according to the preparation method, a protein preparation can be obtained through rapid and accurate extraction, the performance of hair care products can be effectively improved, and the content of disulfide bonds in hair is increased.

Description

Technical Field

[0001] The present application relates to the technical field of protein preparation preparation, and in particular to a double protein preparation and its application in hair care products. Background Art

[0002] The main purpose of hair care products is to improve, restore and maintain the conditioning of hair. They are mainly used after shampooing with shampoo or soap. There are two types: rinse with water and leave-in. The purpose is to improve the combing of hair and prevent hair from tangling. When using, let them stay on the hair for a short time and then wash with water to achieve the effect of conditioning the hair. Conditioners are substances that adsorb on the surface of hair to form a coating, making the hair smooth and in good condition overall.

[0003] Currently, protein preparations are added to hair care products, but the added protein preparations cannot effectively increase the disulfide bond content inside the hair, and the hair care effect is poor. Summary of the invention

[0004] The present application provides a dual protein preparation and its application in hair care products to solve the problems of poor hair care performance and low disulfide bond content in hair in the prior art. The prepared protein preparation is used in hair care products to effectively improve the performance of hair care products and increase the disulfide bond content in hair.

[0005] The present application provides a method for preparing a dual protein preparation, the preparation method comprising the following steps: A. Extract collagen; B. Extract keratin; C. Mix collagen and keratin to produce a dual protein preparation.

[0006] Preferably, the collagen extraction process in step A is as follows: a. First, clean and dry the cod skin; b. Add the treated cod skin into a shearing machine and cut it into small pieces, put it into a stainless steel barrel, add enzymolysis solution for enzymolysis, and stir evenly to obtain an enzymolysis mixed slurry; c. Then, the enzymatic mixed slurry is added into an ultrasonic machine for pneumatic ultrasonic extraction to release the aqueous protein to form a cod skin active mixed liquid; d. Finally, acetic acid solution is added to the cod skin active mixed liquid for re-dissolution, and then the acetic acid solution is used as the dialysis external fluid for 10-14 hours, and then distilled water is used as the dialysis external fluid for 12-22 hours. After freeze-drying, collagen is obtained.

[0007] Preferably, the keratin extraction process in step B is as follows: 1) Pre-treat the wool sample; 2) adding the treated wool sample into a grinder for grinding; 3) Add protein extraction buffer and an appropriate amount of protease inhibitor to the ground sample, and stir thoroughly to obtain a protein extract; 4) Add the protein extract into a centrifuge and centrifuge once to obtain a centrifuge; 5) Finally, transfer the supernatant in the centrifuge to a centrifuge tube for secondary centrifugation; 6) Finally, the supernatant is extracted, which is keratin, and the molecular weight of the keratin is 300-3000Da.

[0008] Preferably, in step 2), the grinding speed of the grinder is 200-400 rpm, and the grinding time is 20 min-30 min.

[0009] Preferably, in step 4), the centrifugal speed of the centrifuge is 12000 rpm-14000 rpm, and the centrifugal time is 12 min-16 min.

[0010] Preferably, in step 5), the secondary centrifugation rate of the centrifuge is 8000 rpm-10000 rpm, and the centrifugation time is 5 min-8 min.

[0011] Preferably, the method further comprises the following steps: quantifying the protein in the protein preparation by using the BCA method, and calculating the concentration of the protein in the sample.

[0012] Preferably, a dual protein preparation is used in hair care products. Beneficial Effects

[0013] (1) The preparation method adopted by the present invention can quickly and accurately extract protein preparations. The protein preparations jointly developed with Huzhou Huxing Daily Chemical Co., Ltd. have high protein content. Cod collagen contains higher content of amino acid residues with side groups (-COOH, -NH2, -OH, etc.) that can form hydrogen bonds, salt bonds or peptide bonds, such as glutamic acid, aspartic acid, arginine, serine, hydroxyproline, etc.; wool keratin contains higher content of cysteine / cysteine, and the amino acid composition is similar to the amino acid composition of hair (such as high cysteine / cysteine, glutamate content), which penetrates into the interior of the hair to build disulfide bonds on damaged keratin bonds. The combination of collagen and keratin achieves better hair repair effect. Keratin improves strength through disulfide bond repair mechanism, and collagen synergistically improves hair strength through hydrogen bonds and / or electrostatic interactions; collagen may also have the performance of promoting the adhesion of CMC between hair scale cells and / or CMC in cortical cells. The protein preparation is used in hair care products, which can effectively improve the performance of hair care products and increase the disulfide bond content in hair.

[0014] (2) In the present invention, the protein extraction buffer and protease inhibitor added to the ground product can increase the protein extraction concentration and effectively prevent protein loss.

[0015] (3) In the present invention, the protein extract is subjected to secondary centrifugation, which can quickly separate the protein from the impurities and further increase the protein extraction concentration.

[0016] The above description is only an overview of the technical solution of the embodiment of the present application. In order to more clearly understand the technical means of the embodiment of the present application, it can be implemented in accordance with the contents of the specification. In order to make the above and other purposes, features and advantages of the embodiment of the present application more obvious and easy to understand, the specific implementation methods of the present application are listed below. DETAILED DESCRIPTION

[0017] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0018] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by technicians in the technical field to which this application belongs; the terms used in the specification of the application are only for the purpose of describing specific embodiments and are not intended to limit this application.

[0019] Reference to "embodiments" herein means that a particular feature, structure, or characteristic described in conjunction with the embodiments may be included in at least one embodiment of the present application. The appearance of the phrase "embodiments" in various locations in the specification does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment that is mutually exclusive with other embodiments. It is explicitly and implicitly understood by those skilled in the art that the embodiments described herein may be combined with other embodiments.

[0020] In order to enable those skilled in the art to better understand the solution of the present application, the technical solution in the embodiments of the present application is clearly and completely described below. Embodiment 1;

[0021] The present application provides a method for preparing a dual protein preparation, the preparation method comprising the following steps: A. Extract collagen; B. Extract keratin; C. Mix collagen and keratin to produce a dual protein preparation.

[0022] In this embodiment, the collagen extraction process in step A is as follows: a. First, clean and dry the cod skin; b. Add the treated cod skin into a shearing machine and cut it into small pieces, put it into a stainless steel barrel, add enzymolysis solution for enzymolysis, and stir evenly to obtain an enzymolysis mixed slurry; c. Then, the enzymatic mixed slurry is added into an ultrasonic machine for pneumatic ultrasonic extraction to release the aqueous protein to form a cod skin active mixed liquid; d. Finally, acetic acid solution was added to the cod skin active mixed liquid for re-dissolution, and then the acetic acid solution was used as the dialysis external fluid for 10 hours, and then distilled water was used as the dialysis external fluid for 12 hours. After freeze-drying, collagen was obtained.

[0023] In this embodiment, the keratin extraction process in step B is as follows: 1) Pre-treat the wool sample; 2) adding the treated wool sample into a grinder for grinding; 3) Add protein extraction buffer and an appropriate amount of protease inhibitor to the ground sample, and stir thoroughly to obtain a protein extract; 4) Add the protein extract into a centrifuge and centrifuge once to obtain a centrifuge; 5) Finally, transfer the supernatant in the centrifuge to a centrifuge tube for secondary centrifugation; 6) Finally, the supernatant is extracted, which is keratin, and the molecular weight of the keratin is 300Da.

[0024] In this embodiment, the grinding speed of the grinder in step 2) is 200 rpm and the grinding time is 20 min.

[0025] In this embodiment, in step 4), the centrifugal speed of the centrifuge is 12000 rpm and the centrifugal time is 12 min.

[0026] In this embodiment, in step 5), the secondary centrifugation rate of the centrifuge is 8000 rpm, and the centrifugation time is 5 min.

[0027] In this embodiment, the following steps are also included: protein preparation is quantified by BCA method to calculate the concentration of protein in the sample.

[0028] The amino acid composition of the cod collagen obtained in Example 1 was analyzed. The main amino acids are as follows. The content of glutamic acid, arginine and hydroxyproline obtained by the treatment process is higher: The amino acid composition of the obtained wool keratin was analyzed, and the main amino acids were as follows. The protein obtained by the treatment process has higher contents of cystine, glutamic acid, arginine, etc.: Embodiment 2: The present application provides a method for preparing a dual protein preparation, the preparation method comprising the following steps: A. Extract collagen; B. Extract keratin; C. Mix collagen and keratin to produce a dual protein preparation.

[0029] In this embodiment, the collagen extraction process in step A is as follows: a. First, clean and dry the cod skin; b. Add the treated cod skin into a shearing machine and cut it into small pieces, put it into a stainless steel barrel, add enzymolysis solution for enzymolysis, and stir evenly to obtain an enzymolysis mixed slurry; c. Then, the enzymatic mixed slurry is added into an ultrasonic machine for pneumatic ultrasonic extraction to release the aqueous protein to form a cod skin active mixed liquid; d. Finally, acetic acid solution was added to the cod skin active mixed liquid for re-dissolution, and then the acetic acid solution was used as the dialysis external fluid for 14 hours, and then distilled water was used as the dialysis external fluid for 22 hours. After freeze-drying, collagen was obtained.

[0030] In this embodiment, the keratin extraction process in step B is as follows: 1) Pre-treat the wool sample; 2) adding the treated wool sample into a grinder for grinding; 3) Add protein extraction buffer and an appropriate amount of protease inhibitor to the ground sample, and stir thoroughly to obtain a protein extract; 4) Add the protein extract into a centrifuge and centrifuge once to obtain a centrifuge; 5) Finally, transfer the supernatant in the centrifuge to a centrifuge tube for secondary centrifugation; 6) Finally, the supernatant is extracted, which is keratin, and the molecular weight of the keratin is 3000Da.

[0031] In this embodiment, the grinding speed of the grinder in step 2) is 400 rpm and the grinding time is 30 min.

[0032] In this embodiment, in step 4), the centrifugal speed of the centrifuge is 14000 rpm and the centrifugal time is 16 min.

[0033] In this embodiment, in step 5), the secondary centrifugation rate of the centrifuge is 10000 rpm, and the centrifugation time is 8 min.

[0034] In this embodiment, the following steps are also included: protein preparation is quantified by BCA method to calculate the concentration of protein in the sample.

[0035] The amino acid composition of the cod collagen obtained in Example 2 was analyzed. The main amino acids are as follows. The content of glutamic acid, arginine and hydroxyproline obtained by the treatment process is higher: The amino acid composition of the obtained wool keratin was analyzed, and the main amino acids were as follows. The protein obtained by the treatment process has higher contents of cystine, glutamic acid, arginine, etc.: Embodiment three: The present application provides a method for preparing a dual protein preparation, the preparation method comprising the following steps: A. Extract collagen; B. Extract keratin; C. Mix collagen and keratin to produce a dual protein preparation.

[0036] In this embodiment, the collagen extraction process in step A is as follows: a. First, clean and dry the cod skin; b. Add the treated cod skin into a shearing machine and cut it into small pieces, put it into a stainless steel barrel, add enzymolysis solution for enzymolysis, and stir evenly to obtain an enzymolysis mixed slurry; c. Then, the enzymatic mixed slurry is added into an ultrasonic machine for pneumatic ultrasonic extraction to release the aqueous protein to form a cod skin active mixed liquid; d. Finally, acetic acid solution was added to the cod skin active mixed liquid for re-dissolution, and then the acetic acid solution was used as the dialysis external fluid for 11 hours, and then distilled water was used as the dialysis external fluid for 13 hours. After freeze-drying, collagen was obtained.

[0037] In this embodiment, the keratin extraction process in step B is as follows: 1) Pre-treat the wool sample; 2) adding the treated wool sample into a grinder for grinding; 3) Add protein extraction buffer and an appropriate amount of protease inhibitor to the ground sample, and stir thoroughly to obtain a protein extract; 4) Add the protein extract into a centrifuge and centrifuge once to obtain a centrifuge; 5) Finally, transfer the supernatant in the centrifuge to a centrifuge tube for secondary centrifugation; 6) Finally, the supernatant is extracted, which is keratin, and the molecular weight of the keratin is 1000Da.

[0038] In this embodiment, the grinding speed of the grinder in step 2) is 250 rpm and the grinding time is 22 min.

[0039] In this embodiment, in step 4), the centrifugal speed of the centrifuge is 12500 rpm and the centrifugal time is 13 min.

[0040] In this embodiment, in step 5), the secondary centrifugation speed of the centrifuge is 8500 rpm, and the centrifugation time is 6 min.

[0041] In this embodiment, the following steps are also included: protein preparation is quantified by BCA method to calculate the concentration of protein in the sample.

[0042] The amino acid composition of the cod collagen obtained in Example 3 was analyzed. The main amino acids are as follows. The content of glutamic acid, arginine and hydroxyproline obtained by the treatment process is higher: The amino acid composition of the obtained wool keratin was analyzed, and the main amino acids were as follows. The protein obtained by the treatment process has higher contents of cystine, glutamic acid, arginine, etc.: Embodiment 4: The present application provides a method for preparing a dual protein preparation, the preparation method comprising the following steps: A. Extract collagen; B. Extract keratin; C. Mix collagen and keratin to produce a dual protein preparation.

[0043] In this embodiment, the collagen extraction process in step A is as follows: a. First, clean and dry the cod skin; b. Add the treated cod skin into a shearing machine and cut it into small pieces, put it into a stainless steel barrel, add enzymolysis solution for enzymolysis, and stir evenly to obtain an enzymolysis mixed slurry; c. Then, the enzymatic mixed slurry is added into an ultrasonic machine for pneumatic ultrasonic extraction to release the aqueous protein to form a cod skin active mixed liquid; d. Finally, acetic acid solution was added to the cod skin active mixed liquid for re-dissolution, and then the acetic acid solution was used as the dialysis external fluid for 13 hours, and then distilled water was used as the dialysis external fluid for 21 hours. After freeze-drying, collagen was obtained.

[0044] In this embodiment, the keratin extraction process in step B is as follows: 1) Pre-treat the wool sample; 2) adding the treated wool sample into a grinder for grinding; 3) Add protein extraction buffer and an appropriate amount of protease inhibitor to the ground sample, and stir thoroughly to obtain a protein extract; 4) Add the protein extract into a centrifuge and centrifuge once to obtain a centrifuge; 5) Finally, transfer the supernatant in the centrifuge to a centrifuge tube for secondary centrifugation; 6) Finally, the supernatant is extracted, which is keratin, and the molecular weight of the keratin is 2500Da.

[0045] In this embodiment, the grinding speed of the grinder in step 2) is 350 rpm and the grinding time is 28 min.

[0046] In this embodiment, in step 4), the centrifugal speed of the centrifuge is 13500 rpm and the centrifugal time is 15 min.

[0047] In this embodiment, in step 5), the secondary centrifugation speed of the centrifuge is 9500 rpm, and the centrifugation time is 7 minutes.

[0048] In this embodiment, the following steps are also included: protein preparation is quantified by BCA method to calculate the concentration of protein in the sample.

[0049] The amino acid composition of the cod collagen obtained in Example 4 was analyzed. The main amino acids are as follows. The content of glutamic acid, arginine and hydroxyproline obtained by the treatment process is higher: The amino acid composition of the obtained wool keratin was analyzed, and the main amino acids were as follows. The protein obtained by the treatment process has higher contents of cystine, glutamic acid, arginine, etc.: Embodiment five: The present application provides a method for preparing a dual protein preparation, the preparation method comprising the following steps: A. Extract collagen; B. Extract keratin; C. Mix collagen and keratin to produce a dual protein preparation.

[0050] In this embodiment, the collagen extraction process in step A is as follows: a. First, clean and dry the cod skin; b. Add the treated cod skin into a shearing machine and cut it into small pieces, put it into a stainless steel barrel, add enzymolysis solution for enzymolysis, and stir evenly to obtain an enzymolysis mixed slurry; c. Then, the enzymatic mixed slurry is added into an ultrasonic machine for pneumatic ultrasonic extraction to release the aqueous protein to form a cod skin active mixed liquid; d. Finally, acetic acid solution was added to the cod skin active mixed liquid for re-dissolution, and then the acetic acid solution was used as the dialysis external fluid for 10 hours, and then distilled water was used as the dialysis external fluid for 22 hours. After freeze-drying, collagen was obtained.

[0051] In this embodiment, the keratin extraction process in step B is as follows: 1) Pre-treat the wool sample; 2) adding the treated wool sample into a grinder for grinding; 3) Add protein extraction buffer and an appropriate amount of protease inhibitor to the ground sample, and stir thoroughly to obtain a protein extract; 4) Add the protein extract into a centrifuge and centrifuge once to obtain a centrifuge; 5) Finally, transfer the supernatant in the centrifuge to a centrifuge tube for secondary centrifugation; 6) Finally, the supernatant is extracted, which is keratin, and the molecular weight of the keratin is 1500Da.

[0052] In this embodiment, the grinding speed of the grinder in step 2) is 200 rpm and the grinding time is 30 min.

[0053] In this embodiment, in step 4), the centrifugal speed of the centrifuge is 12000 rpm and the centrifugal time is 16 min.

[0054] In this embodiment, in step 5), the secondary centrifugation speed of the centrifuge is 8000 rpm, and the centrifugation time is 8 min.

[0055] In this embodiment, the following steps are also included: protein preparation is quantified by BCA method to calculate the concentration of protein in the sample.

[0056] The amino acid composition of the cod collagen obtained in Example 5 was analyzed. The main amino acids are as follows. The content of glutamic acid, arginine and hydroxyproline obtained by the treatment process is higher: The amino acid composition of the obtained wool keratin was analyzed, and the main amino acids were as follows. The protein obtained by the treatment process has higher contents of cystine, glutamic acid, arginine, etc.: Embodiment six: The present application provides a method for preparing a dual protein preparation, the preparation method comprising the following steps: A. Extract collagen; B. Extract keratin; C. Mix collagen and keratin to produce a dual protein preparation.

[0057] In this embodiment, the collagen extraction process in step A is as follows: a. First, clean and dry the cod skin; b. Add the treated cod skin into a shearing machine and cut it into small pieces, put it into a stainless steel barrel, add enzymolysis solution for enzymolysis, and stir evenly to obtain an enzymolysis mixed slurry; c. Then, the enzymatic mixed slurry is added into an ultrasonic machine for pneumatic ultrasonic extraction to release the aqueous protein to form a cod skin active mixed liquid; d. Finally, acetic acid solution was added to the cod skin active mixed liquid for re-dissolution, and then the acetic acid solution was used as the dialysis external fluid for 12 hours, and then distilled water was used as the dialysis external fluid for 17 hours. After freeze-drying, collagen was obtained.

[0058] In this embodiment, the keratin extraction process in step B is as follows: 1) Pre-treat the wool sample; 2) adding the treated wool sample into a grinder for grinding; 3) Add protein extraction buffer and an appropriate amount of protease inhibitor to the ground sample, and stir thoroughly to obtain a protein extract; 4) Add the protein extract into a centrifuge and centrifuge once to obtain a centrifuge; 5) Finally, transfer the supernatant in the centrifuge to a centrifuge tube for secondary centrifugation; 6) Finally, the supernatant is extracted, which is keratin, and the molecular weight of the keratin is 2000Da.

[0059] In this embodiment, the grinding speed of the grinder in step 2) is 300 rpm and the grinding time is 25 min.

[0060] In this embodiment, in step 4), the centrifugal speed of the centrifuge is 13000 rpm and the centrifugal time is 14 min.

[0061] In this embodiment, in step 5), the secondary centrifugation speed of the centrifuge is 9000 rpm, and the centrifugation time is 6 min.

[0062] In this embodiment, the following steps are also included: protein preparation is quantified by BCA method to calculate the concentration of protein in the sample.

[0063] The amino acid composition of the cod collagen obtained in Example 6 was analyzed. The main amino acids are as follows. The content of glutamic acid, arginine and hydroxyproline obtained by the treatment process is higher: The amino acid composition of the obtained wool keratin was analyzed, and the main amino acids were as follows. The protein obtained by the treatment process has higher contents of cystine, glutamic acid, arginine, etc.: Among them, the cod is selected from the Bohai Sea area of ​​China; the wool is selected from the unique Chinese breed - Hu sheep.

[0064] Experimental example: Ellman's color reaction for determination of total sulfhydryl groups and disulfide bonds in proteins: 1. Add 10 μL of Ellman's reagent to each protein solution and react at 25 °C in the dark for 1 h. Use the mixed solution without sample as a blank control.

[0065] 2. Centrifugation: The developed peptide solution was centrifuged at 13600 × g for 10 min, and the supernatant was used for the next step; 3. Absorbance measurement: Using a microplate reader and a transparent 96-well plate, 200 μL of each sample was aspirated and the absorbance of the mixture was measured at 412 nm; 4. Free thiol conversion: Extinction coefficient is 13600M −1 cm −1 The same method is used to determine the total thiol content, but 8 mol / L urea is added to the buffer solution. The calculation formula for free or total thiol and disulfide bonds is as follows: Where D is the dilution factor (diluted to the corresponding multiple so that the absorbance is controlled between 0.2-0.8), A 412 is the absorbance of the sample minus the blank control, C is the concentration of the peptide solution, Ct is the total thiol concentration, and Cf is the free thiol concentration.

[0066] The data obtained is as follows: The protein preparation of the present invention was added to shampoo containing 15% sodium laureth sulfate, 7% cocamidopropyl betaine, an appropriate amount of pH adjuster, an appropriate amount of sodium chloride, an appropriate amount of essence, and the balance of water. After the volunteers used the shampoo, they scored the perception of reduced hair breakage with a needle. If the perception of reduced hair breakage was obvious, it was scored as 10 points, and if the perception was not obvious, it was scored as 1 point. The scoring data were as follows: As can be seen from the above table, the dual protein preparation prepared in Example 6 is used in hair care products. Volunteers perceive less hair breakage, and the dual protein has less hair breakage than the blank and the single protein. In the present invention, the protein extraction buffer and protease inhibitor added to the ground product can increase the protein extraction concentration and effectively prevent the loss of protein. In the present invention, the protein extract is subjected to secondary centrifugation, which can quickly separate the protein from impurities and further increase the protein extraction concentration.

[0067] In summary, the preparation method adopted by the present invention can quickly and accurately extract and obtain a protein preparation. The protein preparation is jointly developed with Huzhou Huxing Daily Chemical Co., Ltd. The extracted protein preparation has a high protein content. Cod collagen contains a higher content of amino acid residues with side groups (-COOH, -NH2, -OH, etc.) that can form hydrogen bonds, salt bonds or peptide bonds, such as glutamic acid, aspartic acid, arginine, serine, etc.; wool keratin contains a higher content of cystine / cysteine, and the amino acid composition is similar to the amino acid composition of hair. It penetrates into the interior of the hair and constructs disulfide bonds on damaged keratin bonds. The combination of collagen and keratin achieves a better hair repair effect. Keratin improves strength from a disulfide bond repair mechanism, and collagen synergistically improves hair strength from hydrogen bonds and or electrostatic interactions; collagen may also have the performance of promoting the adhesion of CMC between hair scale cells and or CMC in cortical cells. The protein preparation is used in hair care products, which can effectively improve the performance of hair care products and increase the disulfide bond content inside the hair.

[0068] As described above, the above embodiments are only used to illustrate the technical solutions of the present application, rather than to limit them. Although the present application has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present application.

Claims

1. A method for preparing a dual protein preparation, characterized in that: The preparation method comprises the following steps: A. Extract collagen; B. Extract keratin; C. Mix collagen and keratin to produce a dual protein preparation.

2. The method for preparing a dual protein preparation according to claim 1, characterized in that: The collagen extraction process in step A is as follows: a. First, clean and dry the cod skin; b. Add the treated cod skin into a shearing machine and cut it into small pieces, put it into a stainless steel barrel, add enzymolysis solution for enzymolysis, and stir evenly to obtain an enzymolysis mixed slurry; c. Then, the enzymatic mixed slurry is added into an ultrasonic machine for pneumatic ultrasonic extraction to release the aqueous protein to form a cod skin active mixed liquid; d. Finally, acetic acid solution is added to the cod skin active mixed liquid for re-dissolution, and then the acetic acid solution is used as the dialysis external fluid for 10-14 hours, and then distilled water is used as the dialysis external fluid for 12-22 hours. After freeze-drying, collagen is obtained.

3. The method for preparing a dual protein preparation according to claim 1, characterized in that: The keratin extraction process in step B is as follows: 1) Pre-treat the wool sample; 2) adding the treated wool sample into a grinder for grinding; 3) Add protein extraction buffer and an appropriate amount of protease inhibitor to the ground sample, and stir thoroughly to obtain a protein extract; 4) Add the protein extract into a centrifuge and centrifuge once to obtain a centrifuge; 5) Finally, transfer the supernatant in the centrifuge to a centrifuge tube for secondary centrifugation; 6) Finally, the supernatant is extracted, which is keratin, and the molecular weight of the keratin is 300-3000Da.

4. The method for preparing a dual protein preparation according to claim 3, characterized in that: In the step 2), the grinding speed of the grinder is 200-400 rpm, and the grinding time is 20-30 min.

5. The method for preparing a dual protein preparation according to claim 3, characterized in that: In the step 4), the centrifugal speed of the centrifuge is 12000 rpm-14000 rpm, and the centrifugal time is 12 min-16 min.

6. The method for preparing a dual protein preparation according to claim 3, characterized in that: In the step 5), the secondary centrifugal speed of the centrifuge is 8000 rpm-10000 rpm, and the centrifugal time is 5 min-8 min.

7. The method for preparing a dual protein preparation according to claim 1, characterized in that: The method also includes the following steps: using the BCA method to quantify the protein in the protein preparation, and calculating the concentration of the protein in the sample.

8. A dual protein preparation, characterized in that The protein preparation is used in hair care products.

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