Composite micro-capsule powder with gastric mucosa repairing effect as well as preparation method and application of composite micro-capsule powder

Abstract

The invention provides composite micro-capsule powder with a gastric mucosa repairing effect as well as a preparation method and application thereof. The composite micro-capsule powder is prepared from the following components in parts by mass: 300 to 380 parts of krill and sea buckthorn seed mixed oil, 50 to 150 parts of sea buckthorn fruit oil, 10 to 50 parts of tremella polysaccharide and 8 to 30 parts of hericium erinaceus polysaccharide. The preparation method comprises the following steps: firstly, mixing krill powder and sea buckthorn seed powder, extracting to obtain krill and sea buckthorn seed mixed oil, then mixing the krill and sea buckthorn seed mixed oil with sea buckthorn fruit oil, and continuously adding an emulsifier and tremella polysaccharide to prepare a mixed microemulsion; meanwhile, a mixed water phase composed of hericium erinaceus polysaccharide and a mixed wall material is prepared, the mixed micro-emulsion is added into the mixed water phase, and then spray drying is conducted to prepare the composite micro-capsule powder. The composite micro-capsule powder prepared by the invention not only has a good gastric mucosa repairing effect, alleviates gastric mucosa damage and promotes gastric mucosa repairing, but also is environment-friendly, non-toxic and free of side effects, has better bioavailability, and can be used for being prepared into multiple dosage forms such as powder, tablets, hard capsules, soft capsules and the like to exert better effects.

Description

Technical Field

[0001] The present application relates to the technical field of food and drug processing, and in particular to a composite microcapsule powder having the function of repairing gastric mucosa, and a preparation method and application thereof. Background Art

[0002] As people's living standards improve, work pressure and irregular life have become the norm. Workplace entertainment and social life have become more frequent, gradually changing people's eating habits and quietly affecting their physical health. Excessive drinking or long-term drinking can cause acute or chronic damage to the gastric mucosa; spicy food, overeating, drugs, etc. will increase the burden on the stomach, causing gastric mucosal damage and then developing into chronic superficial gastritis, gastric ulcers and even gastric cancer. Therefore, regular treatment of early gastric mucosal damage can not only inhibit the progression of gastric disease, but also has important significance for preventing gastric mucosal diseases.

[0003] Currently, there are many drugs for treating gastric diseases, mainly Western medicine, which has the advantage of quick effect, but is highly dependent and has large side effects. At the same time, the drugs can also cause stomach damage, which poses a great hidden danger to patients with chronic gastric diseases.

[0004] In addition, for gastric mucosal damage caused by alcohol, spicy food, and edible drugs, traditional gastric drug treatment will further stimulate the gastric mucosa and cause chronic mucositis, so gastric mucosal damage should be mainly treated with warming and maintenance to control the disease within the controllable range in the early stage. Therefore, at this stage, there is an urgent need for a more environmentally friendly, healthy, non-toxic, non-addictive product that can repair gastric mucosa to provide to consumers. Summary of the invention

[0005] In order to solve the above problems, the present invention provides a composite microcapsule powder having the function of repairing gastric mucosa and its preparation method and application. In the prior art, krill oil is a substance rich in phospholipids, which can effectively protect the liver, but there is no relevant report on relieving gastric damage and repairing gastric mucosa. The present invention first uses krill powder and sea buckthorn seed powder to extract and obtain krill sea buckthorn seed mixed oil, and then after mixing with sea buckthorn fruit oil, emulsifiers such as mono- and diglycerol fatty acid esters and Tremella polysaccharides are continuously added to obtain a mixed microemulsion, and at the same time, an aqueous phase composed of Hericium erinaceus polysaccharides and mixed wall materials is prepared, and the mixed microemulsion is added to the aqueous phase to obtain a composite microcapsule powder, which can not only be applied to powders, granules, microcapsule powders, hard capsules, tablets and other fields, but also the water-insoluble components can be directly applied to water-based foods. The composite microcapsule powder prepared by the present invention not only solves the technical problem that krill sea buckthorn seed mixed oil, sea buckthorn fruit oil, Hericium erinaceus polysaccharide and Tremella polysaccharide are not miscible with each other, but also can give full play to the efficacy of each component, effectively alleviate gastric damage, promote gastric mucosal repair, and has better bioavailability.

[0006] The invention provides a composite microcapsule powder with a gastric mucosa repairing effect, comprising the following components in parts by mass: 300-380 parts of krill seabuckthorn seed mixed oil, 50-150 parts of seabuckthorn fruit oil, 10-50 parts of tremella polysaccharide, and 8-30 parts of hericium erinaceus polysaccharide.

[0007] Optionally, the krill and sea buckthorn seed mixed oil is prepared by uniformly mixing krill powder and sea buckthorn seed powder, and then extracting the mixture by supercritical CO2;

[0008] The preparation steps of the seabuckthorn fruit oil are as follows: crushing and filtering the seabuckthorn fruit to obtain pulp, adding mixed enzyme a for enzymolysis, and then centrifuging to obtain the oil;

[0009] The preparation steps of the Tremella polysaccharide are as follows: crushing dry Tremella, adding mixed enzyme B and then adding water for a primary extraction, inactivating the enzyme and then performing a secondary extraction, filtering and drying to obtain the Tremella polysaccharide;

[0010] The preparation steps of the Hericium erinaceus polysaccharide are as follows: crushing the Hericium erinaceus, adding water for multiple extractions, and then filtering and drying.

[0011] Optionally, the mass ratio of the krill powder to the sea buckthorn seed powder is 2:(1-4), the extraction pressure is 20-35 MPa, the extraction temperature is 30-45° C., and the extraction time is 1-5 h.

[0012] Optionally, the mixed enzyme a consists of pectinase and pepsin; the pectinase accounts for 0.1-1.5wt% of the mass of the pulp; the pepsin accounts for 0.1-0.5wt% of the mass of the pulp; the enzymolysis time is 3-10h, and the enzymolysis temperature is 30-70°C.

[0013] Optionally, the mixed enzyme consists of cellulase and pectinase; the cellulase accounts for 0.5-2wt% of the mass of tremella powder, and the pectinase accounts for 0.1-1.0wt% of the mass of tremella powder; the mass ratio of dry tremella powder to water is 1:(20-60); the first extraction is performed at a temperature of 30-60°C for 1-3h; the second extraction is performed at a temperature of 60-95°C for 0.5-2h.

[0014] Optionally, in the preparation step of Hericium erinaceus polysaccharide, the mass ratio of Hericium erinaceus powder to water is 1:(10-40); the extraction temperature is 60-85°C, the number of extractions is 3 times, the extraction interval is 1.5h / time, and the drying temperature is 150-200°C.

[0015] The present invention also provides a method for preparing the composite microcapsule powder as described above, and the method for preparing the composite microcapsule powder comprises the following steps:

[0016] (1) After krill seabuckthorn seed mixed oil and seabuckthorn fruit oil are mixed, an emulsifier and Tremella polysaccharide are added, the mixture is heated to a suitable temperature, and stirred evenly to obtain a mixed microemulsion;

[0017] (2) adding Hericium erinaceus polysaccharide to the aqueous phase containing the mixed wall material and mixing until completely dissolved to obtain a mixed aqueous phase;

[0018] (3) adding the mixed microemulsion into the mixed aqueous phase, stirring at high speed and then spray drying to obtain the composite microcapsule powder.

[0019] Optionally, in step (1) of the preparation method, the mass ratio of the krill sea buckthorn seed mixed oil to the sea buckthorn fruit oil is (15-30):7; the emulsifier is mono- and di-glycerol fatty acid esters; the mono- and di-glycerol fatty acid esters account for 0.5-2wt% of the mass of the mixed microemulsion, the tremella polysaccharide accounts for 2-10wt% of the mass of the mixed microemulsion, and the heating temperature is 30-80°C.

[0020] Preferably, the heating temperature in step (1) of the preparation method is 50°C.

[0021] Optionally, in step (2) of the preparation method, the mixed wall material is composed of sodium caseinate and modified starch; the sodium caseinate accounts for 1-4wt% of the mass of the mixed aqueous phase, and the modified starch accounts for 5-20wt% of the mass of the mixed aqueous phase; the mass ratio of Hericium erinaceus polysaccharide to water is 1:(2-5); and the mixing temperature is 80°C;

[0022] In step (3) of the preparation method, the mixed microemulsion is added to the mixed aqueous phase, and after high-speed shear mixing at 3000-5000 rpm for 10-20 minutes, the mixture is spray-dried at 150-180° C. to obtain the composite microcapsule powder.

[0023] The present invention also provides the use of any of the above-mentioned composite microcapsule powders, and / or the composite microcapsule powders prepared by any of the above-mentioned methods for preparing the composite microcapsule powders, in the manufacture of food, health products and drug delivery, wherein the application includes the composite microcapsule powder being made into powders, tablets, hard capsules and soft capsules.

[0024] The mechanism of action of each component in this application

[0025] (1) The mechanism of action of a single component in this application

[0026] The krill and seabuckthorn seed mixed oil used in this application is prepared by mixing krill powder and seabuckthorn seed powder evenly and then extracting them through supercritical CO2, wherein the krill and seabuckthorn seed mixed oil contains the effective ingredients of krill oil and seabuckthorn seed oil. Krill oil is rich in Omega-3 polyunsaturated fatty acids, marine phospholipids, choline, astaxanthin, vitamins, minerals, flavonoids, etc. Omega-3 fatty acids are phospholipid-type Omega-3 polyunsaturated fatty acids, which are anti-inflammatory fatty acids that can maintain a healthy balance of cell membranes, resist toxicity and inflammatory attacks, and can reduce ethanol-induced gastric mucosal inflammation damage. At the same time, krill oil can inhibit the secretion of proinflammatory cytokines (TNF-α, IL-6 and IL-1β), the expression of IκBα / NF-κB signaling pathways and the production of nitric oxide (NO), which has the potential to alleviate ethanol-induced acute gastric mucosal damage. The astaxanthin contained in krill oil is the strongest antioxidant discovered in nature by humans so far. Its antioxidant activity far exceeds that of existing antioxidants. It can effectively scavenge free radicals and reduce the damage of oxidative stress to the gastric mucosa.

[0027] Seabuckthorn seed oil contains nearly 160 kinds of active substances, including various unsaturated fatty acids (α-linolenic acid, linoleic acid, oleic acid, etc.), β-carotene, carotenoids and their derivatives, natural vitamin E, flavonoids, sterols, etc. The ratio of α-linolenic acid: linoleic acid in seabuckthorn seed oil is basically 1:1, which is in line with the balance of essential fatty acid intake advocated by the latest lipid nutrition theory. Seabuckthorn seed oil has various functions such as preventing cardiovascular and cerebrovascular diseases, anti-radiation, anti-aging, protecting liver damage, and reducing damage from ulcerative colitis. The fatty acids contained in it can inhibit the total gastric acid excretion and increase the gastric mucus secretion, protect the gastric mucosa, treat gastric ulcers, inhibit gastric emptying, inhibit the movement of gastric smooth muscle, reduce gastric smooth muscle spasm in gastric ulcers, and have a certain analgesic effect.

[0028] Furthermore, the krill powder and sea buckthorn seed powder used in the present application are used as raw materials for supercritical CO2 extraction to prepare the krill and sea buckthorn seed mixed oil, which can better retain the effective content of astaxanthin in the krill oil and play a good antioxidant effect.

[0029] The seabuckthorn fruit oil used in the present application is an oily liquid made from selected seabuckthorn fruit as raw material, and has a unique fatty acid composition, including rich oleic acid (Omega-9), linoleic acid (Omega-6), linolenic acid (Omega-3), palmitoleic acid (Omega-7), etc., and its palmitoleic acid (Omega-7) content is 19.4%-38.5%, which is significantly higher than general vegetable oil. The seabuckthorn fruit pulp oil in the present application has the effects of anti-inflammatory, prevention and treatment of atherosclerosis, protection of the inner mucosal system, improvement of dry eye syndrome, etc., and can also treat skin and mucosal damage, gastric ulcer and other diseases. The unique Omega-7 fatty acid of seabuckthorn fruit oil can reduce the expression of interleukin 6 (IL-6) gene and peroxisome proliferator-activated receptor (PPAR) gene, inhibit NFKB pathway, thereby reducing the expression of inflammatory factors, achieving the purpose of anti-inflammatory, reducing gastric mucosal inflammation, and promoting the healing process of gastric ulcer surface.

[0030] The Hericium erinaceus polysaccharide in the present application, its main components include active substances such as proteins, polypeptides, polysaccharides, terpenes, sterols, etc., which have anti-ulcer and inflammatory, antioxidant and other effects. At the same time, Hericium erinaceus can reduce the swelling degree of gastric tissue and gastric bleeding rate in rats, inhibit the degree of gastric mucosal lesions in rats, and the polysaccharide components of Hericium erinaceus can improve the circulation of gastric mucosa, reduce gastric microvascular damage and reduce microvascular permeability.

[0031] The Tremella used in the present application is a medicinal and edible fungus, which is pure natural, non-toxic, safe, has no side effects, and can be taken for a long time. The chemical components in Tremella include protein and amino acids, enzymes and polysaccharides. Tremella polysaccharide is the main active ingredient of Tremella, which has the effects of regulating immune activity and anti-ulcer, and can inhibit gastric ulcers. Its mechanism of action is mainly acid-reducing, and it further protects the gastric mucosa and inhibits the activity of pepsin.

[0032] (2) Mechanism of synergistic effect between components in this application

[0033] The mixed microemulsion system composed of seabuckthorn seed mixed oil and seabuckthorn fruit oil in the present application is rich in fat and fatty acids, which can not only inhibit gastric acid secretion, but also stimulate the release of various gastrointestinal hormones such as gastric inhibitory peptide, secretin, and intestinal glucagon in the proximal small intestine, among which glucagon can protect the gastric mucosa, secretin and cholecystokinin can further stimulate the epithelial cells on the surface of the gastric mucosa to secrete mucus and enhance the ability to resist damage; at the same time, the unique anti-inflammatory ingredients phospholipid-type Omega-3 fatty acids and Omega-7 fatty acids contained in krill oil and seabuckthorn fruit oil can reduce the inflammatory response caused by gastric mucosal damage, and seabuckthorn seed oil can assist in protecting gastric mucosal damage. Krill oil, seabuckthorn fruit oil and the fatty acids contained in seabuckthorn seed oil can also inhibit the total gastric acid excretion and increase the gastric mucus secretion, synergistically protect the gastric mucosa, reduce the degree of gastric mucosal damage, accelerate the healing process of gastric ulcers, and relieve the pain caused by gastric mucosal damage. At the same time, adding Tremella polysaccharide in the process of preparing the mixed microemulsion can also significantly improve the system stability and uniform dispersion of the mixed microemulsion, and help the mixed microemulsion and the mixed aqueous phase containing Hericium erinaceus polysaccharide to have better dispersion and stability during the combination process.

[0034] The krill seabuckthorn seed mixed oil and seabuckthorn seed oil used in this application are both in oily liquid state, while the Hericium erinaceus polysaccharide and Tremella fuciformis polysaccharide are both in powder state. The above two types of raw materials are insoluble in each other and cannot be further utilized. This application can not only prepare the above composite microcapsule powder into a composite microcapsule powder with good synergistic effect, but also apply it to the fields of powder, granules, hard capsules, soft capsules, tablets, etc., and the original water-insoluble components can be directly applied to water-based foods, which have better bioavailability and can also achieve better synergistic effects.

[0035] The beneficial effects of this application include but are not limited to:

[0036] 1. The present invention firstly provides a composite microcapsule powder for repairing gastric mucosa, with scientific content ratio and synergistic cooperation of various components to repair gastric mucosa; wherein Tremella polysaccharide can form a protective film on the surface of gastric mucosa, effectively isolating direct damage to gastric mucosa by external stimuli such as gastric acid and food, and can play a significant anti-inflammatory role in combination with krill oil Omega-3 fatty acids, seabuckthorn fruit oil Omega-7 fatty acids, seabuckthorn seed oil flavonoid active substances and Hericium erinaceus polysaccharide, thereby reducing gastric mucosal inflammation damage; secondly, krill oil, seabuckthorn fruit oil, seabuckthorn seed oil are rich in fatty acids, Vitamins, minerals and other nutrients can provide necessary nutrients for further repair of gastric mucosa, and cooperate with Hericium erinaceus polysaccharide to further promote the proliferation and differentiation of gastric mucosal cells, and accelerate the healing and regeneration of damaged gastric mucosa. Furthermore, krill oil contains the most effective pure natural antioxidant astaxanthin. The mixed extraction of krill powder and sea buckthorn seed powder can increase the astaxanthin content, which, together with the antioxidant ingredients such as vitamin C and vitamin E in sea buckthorn fruit oil and sea buckthorn seed oil, can remove free radicals in the body and reduce the damage of oxidative stress to gastric mucosa. At the same time, combined with Hericium erinaceus polysaccharide and Tremella polysaccharide, it has the effect of further enhancing the body's immunity.

[0037] 2. The present invention has a significant improvement effect on alcoholic gastric mucosal damage. Through the combined effects of multiple mechanisms such as film-forming protection, anti-inflammatory effects, nutritional support, and promotion of gastric mucosal repair, comprehensive protection of the gastric mucosa is achieved. The present invention can further accelerate the repair process of damaged gastric mucosa and shorten the course of the disease through the synergistic effect of multiple ingredients; through mechanisms such as enhancing immunity and promoting cell regeneration, it helps to reduce the recurrence of gastric mucosal diseases.

[0038] 3. The raw materials provided by the present invention are all natural food raw materials, which are environmentally friendly, healthy, non-toxic, non-addictive, and not only have a good effect on repairing gastric mucosa, but also have better bioavailability, and are not limited by dosage forms. They can be made into various effective dosage forms such as powders, tablets, microcapsules, hard capsules, soft capsules, etc., and exert excellent effects. DETAILED DESCRIPTION

[0039] In order to more clearly illustrate the overall concept of the present invention, a detailed description is given below by way of examples.

[0040] In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Therefore, the protection scope of the present invention is not limited to the specific embodiments disclosed below.

[0041] Hereinafter, the composite microcapsule powder having the function of repairing gastric mucosa and the preparation method and application thereof of the present invention will be described in detail in combination with exemplary embodiments.

[0042] Example 1

[0043] The preparation steps of a composite microcapsule powder for repairing gastric mucosa are as follows:

[0044] (I) Raw material preparation process stage:

[0045] ① Preparation of krill and sea buckthorn seed mixed oil: Antarctic krill is frozen and crushed to obtain Antarctic krill powder, sea buckthorn seeds are crushed to obtain sea buckthorn seed powder, Antarctic krill powder and sea buckthorn seed powder are uniformly mixed at a mass ratio of 2:1, and then supercritical CO2 extraction is performed at an extraction pressure of 25 MPa, an extraction temperature of 35°C, and an extraction time of 2 hours to obtain krill and sea buckthorn seed mixed oil;

[0046] ② Preparation of seabuckthorn fruit oil: Select and wash seabuckthorn fruits, crush and beat with a crusher, filter to obtain pulp, add 0.5wt% pectinase and 0.15wt% pepsin for enzymolysis for 6 hours at a temperature of 55°C, and then centrifuge to obtain seabuckthorn fruit oil (the above proportions are all proportions of the total mass of the pulp);

[0047] ③ Preparation of Tremella polysaccharide: Grind dry Tremella to obtain dry Tremella powder, add 1.2wt% cellulase and 0.5wt% pectinase, and add water once for extraction according to the mass ratio of dry Tremella powder to water of 1:40, the extraction temperature is 55°C, the extraction time is 2h, after the enzyme is inactivated, the secondary extraction is continued at 95°C for 1h, the Tremella polysaccharide extract is obtained after filtering, and the Tremella polysaccharide extract is obtained by spray drying at 160°C (the above proportions are all proportions of the total mass of the dry Tremella powder);

[0048] ④ Preparation of Hericium erinaceus polysaccharide: crush Hericium erinaceus, and extract with water according to the mass ratio of Hericium erinaceus powder to water of 1:30, the extraction temperature is 75°C, the extraction times are 3 times, and the extraction interval is 1.5h / time, and the Hericium erinaceus polysaccharide extract is filtered to obtain the Hericium erinaceus polysaccharide extract, and spray-dried at 160°C to obtain Hericium erinaceus polysaccharide;

[0049] (II) Preparation process of composite microcapsule powder:

[0050] ① Preparing a mixed microemulsion: 340 parts of krill seabuckthorn seed mixed oil and 140 parts of seabuckthorn fruit oil were mixed, and 1wt% of mono- and diglycerol fatty acid esters and 30 parts of Tremella polysaccharide were added, heated to 50° C., and stirred to mix uniformly to prepare a mixed microemulsion (the above proportions are all proportions of the total mass of the mixed microemulsion);

[0051] ②Preparing a mixed aqueous phase: adding 3wt% sodium caseinate and 15wt% modified starch (the mass ratio of maltodextrin to sodium starch octenyl succinate is 3:5) as a mixed wall material to 30 parts of water, and then adding 15 parts of Hericium erinaceus polysaccharide as a filler; heating to 80°C and stirring until completely dissolved to obtain a mixed aqueous phase (the above proportions are all proportions of the total mass of the mixed aqueous phase);

[0052] ③ The microemulsion was slowly added into the aqueous phase, high-speed shear mixing was performed at 4000 rpm for 15 minutes, and composite microcapsule powder was prepared after spray drying at 160°C.

[0053] Example 2

[0054] The preparation steps of a composite microcapsule powder for repairing gastric mucosa are as follows:

[0055] (I) Raw material preparation process stage:

[0056] ① Preparation of krill and sea buckthorn seed mixed oil: Antarctic krill is frozen and crushed to obtain Antarctic krill powder, and sea buckthorn seeds are crushed to obtain sea buckthorn seed powder. Antarctic krill powder and sea buckthorn seed powder are uniformly mixed at a mass ratio of 1:1, and then put into an extraction kettle. The extraction pressure is 35Mpa, the extraction temperature is 45°C, and the extraction time is 5 hours to obtain krill and sea buckthorn seed mixed oil;

[0057] ② Preparation of seabuckthorn fruit oil: Select and wash seabuckthorn fruits, crush and beat with a crusher, filter to obtain pulp, add 1.5wt% pectinase and 0.5wt% pepsin to perform enzymolysis for 10 hours at a temperature of 70°C, and then centrifuge to obtain seabuckthorn fruit oil (the above proportions are all proportions of the total mass of the pulp);

[0058] ③ Preparation of Tremella polysaccharide: Grind dry Tremella to obtain dry Tremella powder, add 2wt% of cellulase and 1wt% of pectinase, and add water once for extraction according to the mass ratio of dry Tremella powder to water of 1:60, the extraction temperature is 60°C, the extraction time is 1h, and after the enzyme is inactivated, the secondary extraction is continued at 95°C for 1h, and the Tremella polysaccharide extract is obtained after filtering, and the Tremella polysaccharide extract is spray-dried at 160°C to obtain Tremella polysaccharide (the above proportions are all proportions of the total mass of the dry Tremella powder);

[0059] ④ Preparation of Hericium erinaceus polysaccharide: crush Hericium erinaceus, and extract with water according to the mass ratio of Hericium erinaceus powder to water of 1:40, the extraction temperature is 60°C, the extraction times are 3 times, and the extraction interval is 1.5h / time, and the Hericium erinaceus polysaccharide extract is filtered to obtain the Hericium erinaceus polysaccharide extract, and spray-dried at 160°C to obtain Hericium erinaceus polysaccharide;

[0060] (II) Preparation process of composite microcapsule powder:

[0061] ① Preparing a mixed microemulsion: 380 parts of krill seabuckthorn seed mixed oil and 150 parts of seabuckthorn fruit oil were mixed, and 2 wt% of mono- and diglycerol fatty acid esters and 50 parts of Tremella polysaccharide were added, heated to 80° C., and stirred to mix uniformly to prepare a mixed microemulsion (the above proportions are all proportions of the total mass of the mixed microemulsion);

[0062] ②Preparing a mixed aqueous phase: adding 4wt% sodium caseinate and 20wt% modified starch (the mass ratio of maltodextrin to sodium starch octenyl succinate is 3:5) as a mixed wall material to 150 parts of water, and then adding 30 parts of Hericium erinaceus polysaccharide as a filler; heating to 80°C and stirring until completely dissolved to obtain a mixed aqueous phase (the above proportions are all proportions of the total mass of the mixed aqueous phase);

[0063] ③ The microemulsion was slowly added into the aqueous phase, high-speed shear mixing was performed at 3000 rpm for 30 minutes, and composite microcapsule powder was prepared after spray drying at 160°C.

[0064] Example 3

[0065] The preparation steps of a composite microcapsule powder for repairing gastric mucosa are as follows:

[0066] Raw material preparation process stage:

[0067] ① Preparation of krill and sea buckthorn seed mixed oil: Antarctic krill is frozen and crushed to obtain Antarctic krill powder, and sea buckthorn seeds are crushed to obtain sea buckthorn seed powder. Antarctic krill powder and sea buckthorn seed powder are uniformly mixed at a mass ratio of 1:2, and then put into an extraction kettle. The extraction pressure is 20Mpa, the extraction temperature is 30°C, and the extraction time is 1 hour to obtain krill and sea buckthorn seed mixed oil;

[0068] ② Preparation of seabuckthorn fruit oil: Select and wash seabuckthorn fruits, crush and beat with a crusher, filter to obtain pulp, add 0.1wt% pectinase and 0.1wt% pepsin for enzymolysis for 3 hours at a temperature of 30°C, and then centrifuge to obtain seabuckthorn fruit oil (the above proportions are all proportions of the total mass of the pulp);

[0069] ③ Preparation of Tremella polysaccharide: Grind dry Tremella to obtain dry Tremella powder, add 0.5wt% of cellulase and 0.1wt% of pectinase, and extract with water at a mass ratio of 1:20 of dry Tremella powder to water, the extraction temperature is 30°C, the extraction time is 1h, and after the enzyme is inactivated, the extraction is continued at 60°C for 0.5h, and the Tremella polysaccharide extract is obtained after filtering, and the Tremella polysaccharide extract is spray-dried at 160°C to obtain Tremella polysaccharide (the above proportions are all proportions of the total mass of the dry Tremella powder);

[0070] ④ Preparation of Hericium erinaceus polysaccharide: crush Hericium erinaceus, and extract with water according to the mass ratio of Hericium erinaceus powder to water of 1:10, the extraction temperature is 60°C, the extraction times are 3 times, and the extraction interval is 1.5h / time, and the Hericium erinaceus polysaccharide extract is filtered to obtain the Hericium erinaceus polysaccharide extract, and spray-dried at 160°C to obtain Hericium erinaceus polysaccharide;

[0071] Composite microcapsule powder preparation process stages:

[0072] ① Preparing a mixed microemulsion: 300 parts of krill seabuckthorn seed mixed oil and 70 parts of seabuckthorn fruit oil were mixed, and 0.5wt% of mono- and diglycerol fatty acid esters and 10 parts of Tremella polysaccharide were added, heated to 30°C, and stirred to mix uniformly to prepare a mixed microemulsion (the above proportions are all proportions of the total mass of the mixed microemulsion);

[0073] ②Preparing a mixed aqueous phase: adding 1wt% sodium caseinate and 5wt% modified starch (the mass ratio of maltodextrin to sodium starch octenylsuccinate is 3:5) as a mixed wall material to 16 parts of water, and then adding 8 parts of Hericium erinaceus polysaccharide as a filler; heating to 80°C and stirring until completely dissolved to obtain a mixed aqueous phase (the above proportions are all proportions of the total mass of the mixed aqueous phase);

[0074] ③ The microemulsion was slowly added into the aqueous phase, high-speed shear mixing was performed at 4000 rpm for 15 minutes, and composite microcapsule powder was prepared after spray drying at 160°C.

[0075] Example 4

[0076] The preparation steps of a composite microcapsule powder for repairing gastric mucosa are as follows:

[0077] Raw material preparation process stage:

[0078] ① Preparation of krill and sea buckthorn seed mixed oil: Antarctic krill is frozen and crushed to obtain Antarctic krill powder, and sea buckthorn seeds are crushed to obtain sea buckthorn seed powder. The Antarctic krill powder and sea buckthorn seed powder are uniformly mixed at a mass ratio of 1.5:1, and then put into an extraction kettle. The extraction pressure is 30Mpa, the extraction temperature is 35°C, and the extraction time is 3 hours to obtain krill and sea buckthorn seed mixed oil;

[0079] ② Preparation of seabuckthorn fruit oil: Select and wash the seabuckthorn fruit, crush and beat it with a crusher, filter it to obtain pulp, add 1.0wt% pectinase and 0.3wt% pepsin to perform enzymolysis for 6 hours at a temperature of 55°C, and then centrifuge to obtain seabuckthorn fruit oil (the above proportions are all proportions of the total mass of the pulp);

[0080] ③ Preparation of Tremella polysaccharide: Grind dry Tremella to obtain dry Tremella powder, add 1.2wt% of cellulase and 0.6wt% of pectinase, and extract with water at a mass ratio of 1:50 of dry Tremella powder to water, the extraction temperature is 55°C, the extraction time is 2h, and the enzyme is inactivated and then the extraction is continued at 95°C for 1h, and the Tremella polysaccharide extract is obtained after filtering, and the Tremella polysaccharide extract is spray-dried at 160°C to obtain Tremella polysaccharide (the above proportions are all proportions of the total mass of the dry Tremella powder);

[0081] ④ Preparation of Hericium erinaceus polysaccharide: crush Hericium erinaceus, and extract with water according to the mass ratio of Hericium erinaceus powder to water of 1:40, the extraction temperature is 75°C, the extraction times are 3 times, and the extraction interval is 1.5h / time, and the Hericium erinaceus polysaccharide extract is filtered to obtain the Hericium erinaceus polysaccharide extract, and spray-dried at 160°C to obtain Hericium erinaceus polysaccharide;

[0082] Composite microcapsule powder preparation process stages:

[0083] ① Preparing a mixed microemulsion: 300 parts of krill seabuckthorn seed mixed oil and 140 parts of seabuckthorn fruit oil were mixed, and 1.5wt% of mono- and diglycerol fatty acid esters and 40 parts of Tremella polysaccharide were added, and the mixture was heated to 60°C, and stirred and mixed to obtain a mixed microemulsion (the above ratio is the ratio of the total mass of the mixed microemulsion);

[0084] ②Preparing a mixed aqueous phase: adding 4wt% sodium caseinate and 15wt% modified starch (the mass ratio of maltodextrin to sodium starch octenyl succinate is 3:5) as a mixed wall material to 54 parts of water, and then adding 18 parts of Hericium erinaceus polysaccharide as a filler; heating to 80°C and stirring until completely dissolved to obtain a mixed aqueous phase (the above proportions are all proportions of the total mass of the mixed aqueous phase);

[0085] ③ The microemulsion was slowly added into the aqueous phase, high-speed shear mixing was performed at 4000 rpm for 15 minutes, and composite microcapsule powder was prepared after spray drying at 160°C.

[0086] Example 5

[0087] In this embodiment, another comparative composite microcapsule powder is prepared by the same preparation method as that in Example 1. The difference from Example 1 is that, in the raw material preparation stage, in the step of preparing the krill and sea buckthorn seed mixed oil, Antarctic krill powder and sea buckthorn seed powder are uniformly mixed in a mass ratio of 1:5 to prepare the krill and sea buckthorn seed mixed oil; in the composite microcapsule powder preparation process stage, in the process of preparing the mixed microemulsion, mono- and di-glycerol fatty acid esters are not added, and only the tremella polysaccharide component is added to prepare the composite microcapsule powder.

[0088] Example 6

[0089] In this embodiment, another comparative composite microcapsule powder is prepared by the same preparation method as in Example 1. The difference from Example 1 is that, in the composite microcapsule powder preparation process stage, krill sea buckthorn seed mixed oil, sea buckthorn fruit oil, mono- and diglycerol fatty acid esters, tremella polysaccharide, sodium caseinate, modified starch, Hericium erinaceus polysaccharide, and aqueous phase are directly subjected to simple physical mixing, and spray drying at 160° C. is performed to prepare the composite microcapsule powder.

[0090] Comparative Example 1

[0091] In this comparative example, another comparative composite microcapsule powder is prepared by the same preparation method as in Example 1. The difference from Example 1 is that in the composite microcapsule powder preparation process stage, yam polysaccharide (commercially available) is used instead of tremella polysaccharide prepared in the raw material preparation process stage to prepare the composite microcapsule powder.

[0092] Comparative Example 2

[0093] In this comparative example, another comparative composite microcapsule powder is prepared by the same preparation method as in Example 1. The difference from Example 1 is that in the composite microcapsule powder preparation process stage, Poria polysaccharide (commercially available) is used to replace Hericium erinaceus polysaccharide prepared in the raw material preparation process stage to prepare the composite microcapsule powder.

[0094] Comparative Example 3

[0095] In this comparative example, another comparative composite microcapsule powder was prepared by the same preparation method as in Example 1. The difference from Example 1 is that, in the composite microcapsule powder preparation process stage, perilla seed oil was used instead of sea buckthorn fruit oil to prepare the composite microcapsule powder.

[0096] Comparative Example 4

[0097] In this comparative example, another comparative composite microcapsule powder was prepared by the same preparation method as in Example 1. The difference from Example 1 is that, in the raw material preparation process stage, krill oil was prepared only by krill powder in the preparation process of krill and sea buckthorn seed mixed oil, and no sea buckthorn seed powder was added; in the composite microcapsule powder preparation process stage, krill oil was used instead of krill and sea buckthorn seed mixed oil to prepare the composite microcapsule powder.

[0098] Comparative Example 5

[0099] In this comparative example, another comparative composite microcapsule powder was prepared by the same preparation method as in Example 1. The difference from Example 1 was that, in the raw material preparation process stage, wolfberry seeds were used to replace the krill powder component, and wolfberry seed powder and sea buckthorn seed powder were extracted to prepare wolfberry seed and sea buckthorn seed mixed oil, and in the composite microcapsule powder preparation process stage, wolfberry seed and sea buckthorn seed mixed oil was used to replace the krill and sea buckthorn seed mixed oil to prepare the composite microcapsule powder.

[0100] Comparative Example 6

[0101] In this comparative example, another comparative composite microcapsule powder was prepared by the same preparation method as in Example 1. The difference from Example 1 is that, in the composite microcapsule powder preparation process stage, no Tremella polysaccharide was added during the preparation of the mixed microemulsion, and only mono- and diglycerol fatty acid ester components were added to prepare the composite microcapsule powder.

[0102] Comparative Example 7

[0103] In this comparative example, another comparative composite microcapsule powder was prepared by the same preparation method as in Example 1. The difference from Example 1 is that during the composite microcapsule powder preparation process, Hericium erinaceus polysaccharide was not added to prepare the composite microcapsule powder.

[0104] Test 1: The composite microcapsule powder of the present invention has an improving effect on alcoholic gastric mucosal damage. The following are the results of animal experiments and human experiments:

[0105] (I) Animal experiment verification results:

[0106] 1. Raw material preparation: Take the composite microcapsule powder prepared in Examples 1-6 and Comparative Examples 1-7.

[0107] 2. Grouping: Healthy male Wistar mice were selected, and after adaptive feeding for 1 week, they were randomly divided into a blank group, a control group, Example 1-6 groups, and Comparative Example 1-7 groups, with 10 mice in each group.

[0108] 3. Administration: The blank group and the model group were given an equal amount of water, and the Example 1-6 and the Comparative Example 1-7 groups were given (2 g / kg) by gavage, respectively, once a day for 6 consecutive days. After the administration on the 6th day, they were fasted for 24 hours, but water was not withheld. On the 7th day, except for the blank group, the other groups were gavaged with 80% ethanol 0.1 ml / 10 g 90 minutes after administration, and the animals were killed 4 hours later, and the stomach tissues were taken for subsequent experiments.

[0109] 4. Experimental Results

[0110] 4.1 Normal rat body weight changes

[0111] During the experiment, the body weight of rats was recorded every day, and the body weight of mice (unit / g) was not much different from that of the blank group.

[0112] Table 1. Statistics of rat body weight

[0113]

[0114]

[0115] 4.2 Mouse gastric juice pH test

[0116] Table 2. Statistics of pH value of mouse gastric juice

[0117] Group pH Blank Group 3.3±0.9 Control group 6.6±2.5 Example 1 4.2±1.4 Example 2 4.8±1.6 Example 3 4.9±0.9 Example 4 5.0±2.5 Example 5 5.1±2.9 Example 6 5.3±1.5 Comparative Example 1 5.4±1.2 Comparative Example 2 5.5±2.4 Comparative Example 3 5.8±1.8 Comparative Example 4 5.8±3.1 Comparative Example 5 5.7±2.1 Comparative Example 6 5.4±1.8 Comparative Example 7 5.5±1.7

[0118] Analysis of experimental results: The pH values ​​of gastric juice of mice in each group are shown in Table 2. The composite microcapsule powders prepared in Examples 1-4 can alleviate the increase in gastric juice pH caused by alcohol gavage, and the composite microcapsule powders prepared in Examples 1-3 have a better improvement effect on the pH value, especially Example 1, which has the most significant improvement effect.

[0119] Compared with Example 1, the improvement effect of Comparative Examples 1-2 is poorer. The reason is that Comparative Example 1 replaces Tremella polysaccharide with yam polysaccharide, and Comparative Example 2 replaces Hericium erinaceus polysaccharide with Poria polysaccharide. The Tremella polysaccharide used in the present application includes protein and amino acids, enzymes and polysaccharides, which have the effects of regulating immune activity and anti-ulcer, can inhibit gastric ulcers, and further protect gastric mucosa and inhibit pepsin activity. In addition, Hericium erinaceus polysaccharide includes active substances such as proteins, polypeptides, polysaccharides, terpenes, sterols, etc., which have anti-ulcer and inflammation, antioxidant effects, etc., can effectively reduce the swelling degree of gastric tissue and gastric bleeding rate in mice, and can also improve the circulation of gastric mucosa.

[0120] Compared with Example 1, the improvement effects of Comparative Examples 3-5 are also poor. The reason is that in Comparative Example 3, perilla seed oil is used instead of sea buckthorn fruit oil, in Comparative Example 4, a mixed oil is prepared by extracting flaxseed and krill powder, and in Comparative Example 5, a mixed oil is prepared by extracting wolfberry seed powder and sea buckthorn seed powder. The sea buckthorn fruit oil used in the present application includes rich palmitoleic acid (Omega-7), which can effectively reduce gastric inflammation and promote the healing process of gastric mucosal damage. At the same time, α-linolenic acid and linoleic acid in sea buckthorn seed oil and Omega-3 polyunsaturated fatty acids in krill oil can effectively reduce gastric mucosal inflammation damage, inhibit total gastric acid excretion and increase gastric mucus secretion, and further protect the gastric mucosa.

[0121] Compared with Example 1, the improvement effect of Comparative Examples 6-7 is also poor, because Comparative Example 6 does not add Tremella polysaccharide, and Comparative Example 7 does not add Hericium erinaceus polysaccharide. The Tremella polysaccharide used in the present application can not only indirectly affect the pH value of gastric juice by improving the body's immunity with kiwi polysaccharide, but the Tremella polysaccharide can also improve the stability and uniform dispersibility of the mixed microemulsion system, which is beneficial to better dispersion of Hericium erinaceus polysaccharide in the mixed aqueous phase to achieve the best effect.

[0122] In Example 5, the component ratio of Antarctic krill powder and sea buckthorn seed powder was changed during the raw material preparation stage, and mono- and di-glycerol fatty acid esters were not added during the composite microcapsule powder preparation process, so the beneficial synergistic effect between the components could not be fully exerted. In Example 6, the components were simply physically mixed during the composite microcapsule powder preparation stage, and the final product obtained had poor stability and uneven mixing, so the beneficial effects between the components could not be fully exerted.

[0123] In summary, some anti-inflammatory ingredients present in the krill sea buckthorn seed mixed oil and sea buckthorn fruit oil in the present application can effectively alleviate the rapid changes in the pH value of gastric juice by reducing gastric inflammation and damage; at the same time, the active ingredients in sea buckthorn seed oil and sea buckthorn fruit oil can effectively cover and protect the gastric mucosa, reduce gastric acid irritation and gastric mucosal damage, and the unsaturated fatty acids and astaxanthin components in krill oil can also contribute to the repair and regeneration of gastric mucosa; in addition, Tremella polysaccharide and kiwi polysaccharide can also indirectly affect the pH value of gastric juice by improving the body's immunity.

[0124] 4.3 MDA content in mouse gastric tissue

[0125] Malondialdehyde (MDA) is produced by lipid peroxidation of polyunsaturated fatty acids and is a marker of oxidative stress. Therefore, the degree of lipid peroxidation and the body's oxidative stress level can be assessed by the content of MDA in tissues (unit: nmol / L).

[0126] Table 3. Statistics of MDA content in mouse stomach tissue

[0127] Group MDA (nmol / L) Blank Group 5.5±2.15 Control group 8.4±3.65 Example 1 6.2±2.19 Example 2 6.8±1.56 Example 3 6.9±1.52 Example 4 7.0±1.64 Example 5 7.2±3.10 Example 6 7.3±3.10 Comparative Example 1 7.6±2.36 Comparative Example 2 7.5±1.58 Comparative Example 3 8.0±1.64 Comparative Example 4 7.9±1.35 Comparative Example 5 8.1±2.01 Comparative Example 6 7.6±1.91 Comparative Example 7 7.7±1.25

[0128] Analysis of experimental results: Compared with the composite microcapsule powders prepared from the other components, the composite microcapsule powders prepared from Examples 1-4 can effectively reduce the content of MDA in gastric mucosal tissue, and the composite microcapsule powders prepared from Examples 1-3 are more effective in reducing the MDA content, especially Example 1 has the best improvement effect, which can effectively alleviate oxidative damage to the gastric mucosa. The main reason is that the krill and sea buckthorn seed mixed oil and sea buckthorn fruit oil used in the present application contain higher content of Omega-3 fatty acids, phospholipids, astaxanthin, vitamins and other bioactive ingredients, have better antioxidant and anti-inflammatory effects, and are beneficial to reducing gastric mucosal oxidation. Sea buckthorn seed oil and sea buckthorn fruit oil also have the function of protecting gastric mucosa and scavenging free radicals, thereby effectively reducing the content of MDA in gastric juice; at the same time, the krill and sea buckthorn seed mixed oil prepared by supercritical CO2 extraction using krill powder and sea buckthorn seed powder as raw materials used in the present application can better retain the effective content of astaxanthin in krill oil and play a good antioxidant effect; and Tremella polysaccharide can further reduce gastric mucosal oxidative damage and help scavenge free radicals through its strong antioxidant effect. Kiwi polysaccharide has the effect of improving blood circulation and further reducing the content of MDA.

[0129] 4.4 Relative expression of TNF-α, IL-1β and IL-6 in mouse gastric tissue

[0130] Ethanol causes gastric damage by directly damaging the gastric mucosa, causing gastric tissue to produce free radicals, reducing the secretion of gastric mucus, and causing related symptoms such as gastric bleeding, and activating the body's immune system, stimulating the production of proinflammatory factors, and the activation of IL-1β and IL-6 will induce the production of TNF-α again, exacerbating the inflammatory response. After 80% ethanol gavage, the relative expression levels of TNF-α, IL-1β and IL-6 (unit: pg / mL) were significantly increased, indicating that alcohol gavage can stimulate the production of proinflammatory factors in rats, and the sample groups can reduce the content of proinflammatory factors, indicating that Example 1-4 groups can effectively reduce inflammation caused by alcohol, and Example 1-3 groups have better improvement effects on inflammatory reactions, especially Example 1 group has the best improvement effect, which can better reduce the probability of gastric inflammation.

[0131] Table 4. Statistics of mouse gastric tissue experimental data

[0132]

[0133]

[0134] The above animal experiments show that the composite microcapsule powder can protect the gastric mucosa by alleviating alcohol-induced oxidative damage to gastric mucosal epithelial cells, reducing gastric tissue inflammation, and regulating gastric juice pH.

[0135] (II) Feedback from human test experiment:

[0136] 1. Raw material preparation: Take the composite microcapsule powder prepared in Examples 1-6 and Comparative Examples 1-7.

[0137] 2. Grouping: 130 men with drinking habits were selected and randomly divided into 13 groups according to their drinking habits, drinking amount, drinking status, etc., with 10 people in each group.

[0138] 3. Experimental method: One hour after lunch, Group 1 to Group 6 took 20 g of the composite microcapsule powder prepared by Groups 1 to 6 of Examples, and Group 7 to Group 13 took 20 g of the composite powder prepared by Groups 1 to 7 of Comparative Examples, respectively. Then, they drank a total of 300 mL of liquor (alcohol content of 42°) within 30 minutes. After 2 hours, they scored using the questionnaire and took a weighted average of the final scores.

[0139] Table 5. Statistics of human test data

[0140]

[0141]

[0142] Questionnaire analysis: From the human trial questionnaire feedback results in Table 5 above, it can be seen that the data in Examples 1-4 all have a certain alleviating effect on alcoholic gastric damage, and the improvement effect of Examples 1-3 is more significant, especially Example 1 has the best alleviating effect on alcoholic gastric damage. By comparison, it can be seen that compared with Example 1, Example 5 has a different ratio of krill powder and sea buckthorn seed powder in the process of preparing krill and sea buckthorn seed mixed oil, and no mono- and diglycerol fatty acid esters are added in the preparation of the mixed microemulsion; Compared with Example 1, Example 6 only physically mixes the components in the composite microcapsule powder preparation process; Compared with Example 1, Comparative Example 1 uses yam polysaccharide (commercially available) instead of Tremella polysaccharide in the composite microcapsule powder preparation process; Compared with Example 1, Comparative Example 2 uses yam polysaccharide (commercially available) instead of Tremella polysaccharide in the composite microcapsule powder preparation process. Poria polysaccharide (commercially available) was used to replace Hericium erinaceus polysaccharide; Comparative Example 3, compared with Example 1, no sea buckthorn fruit oil was added in the preparation process of the composite microcapsule powder; Comparative Example 4, compared with Example 1, only krill oil was used as a raw material in the krill sea buckthorn seed mixed oil; Comparative Example 5, compared with Example 1, wolfberry seed powder was used to replace krill powder, and mixed oil was prepared with sea buckthorn seed powder; Comparative Example 6, compared with Example 1, Tremella polysaccharide was not added during the preparation of the mixed microemulsion; Comparative Example 7, compared with Example 1, Hericium erinaceus polysaccharide was not added in the preparation process of the composite microcapsule powder. The composite microcapsule powder finally prepared by the above comparative experiments is inferior to that of Example 1 in terms of the effect of improving and relieving alcoholic gastric damage, indicating that the composite microcapsule powder prepared by the present application has the effect of significantly improving and relieving alcoholic gastric damage.

[0143] The above is only the embodiment of the present application, and the protection scope of the present application is not limited by these specific embodiments, but is determined by the claims of the present application. For those skilled in the art, the present application can have various changes and variations. Any modification, equivalent replacement, improvement, etc. made within the technical ideas and principles of the present application should be included in the protection scope of the present application.

Claims

1. A composite microcapsule powder having the function of repairing gastric mucosa, characterized in that: The invention comprises the following components in parts by weight: 300-380 parts of krill and seabuckthorn seed mixed oil, 50-150 parts of seabuckthorn fruit oil, 10-50 parts of tremella polysaccharide and 8-30 parts of hericium erinaceus polysaccharide.

2. The composite microcapsule powder according to claim 1, characterized in that: The preparation steps of the krill and sea buckthorn seed mixed oil are as follows: krill powder and sea buckthorn seed powder are uniformly mixed, and then supercritical CO2 extraction is performed to obtain the mixed oil; The preparation steps of the seabuckthorn fruit oil are as follows: crushing and filtering the seabuckthorn fruit to obtain pulp, adding mixed enzyme a for enzymolysis, and then centrifuging to obtain the oil; The preparation steps of the Tremella polysaccharide are as follows: crushing dry Tremella, adding mixed enzyme B and then adding water for a primary extraction, inactivating the enzyme and then performing a secondary extraction, filtering and drying to obtain the Tremella polysaccharide; The preparation steps of the Hericium erinaceus polysaccharide are as follows: crushing the Hericium erinaceus, adding water for multiple extractions, and then filtering and drying.

3. The composite microcapsule powder according to claim 2, characterized in that: The mass ratio of the krill powder to the sea buckthorn seed powder is 2:(1-4), the extraction pressure is 20-35 MPa, the extraction temperature is 30-45° C., and the extraction time is 1-5 hours.

4. The composite microcapsule powder according to claim 2, characterized in that: The mixed enzyme a is composed of pectinase and pepsin; the pectinase accounts for 0.1-1.5wt% of the mass of the pulp; the pepsin accounts for 0.1-0.5wt% of the mass of the pulp; the enzymolysis time is 3-10h, and the enzymolysis temperature is 30-70℃.

5. The composite microcapsule powder according to claim 2, characterized in that: The mixed enzyme consists of cellulase and pectinase; the cellulase accounts for 0.5-2wt% of the mass of the tremella powder, and the pectinase accounts for 0.1-1.0wt% of the mass of the tremella powder; the mass ratio of the dry tremella powder to water is 1:(20-60); the first extraction is performed at a temperature of 30-60°C for 1-3h; and the second extraction is performed at a temperature of 60-95°C for 0.5-2h.

6. The composite microcapsule powder according to claim 2, characterized in that: In the preparation step of the Hericium erinaceus polysaccharide, the mass ratio of Hericium erinaceus powder to water is 1:(10-40); the extraction temperature is 60-85°C, the number of extractions is 3 times, the extraction interval is 1.5h / time, and the drying temperature is 150-200°C.

7. The method for preparing the composite microcapsule powder according to any one of claims 1 to 6, characterized in that: The preparation method of the composite microcapsule powder comprises the following steps: (1) krill seabuckthorn seed mixed oil and seabuckthorn fruit oil are mixed, heated, and then an emulsifier and Tremella polysaccharide are added, and stirred to obtain a mixed microemulsion; (2) adding Hericium erinaceus polysaccharide to the aqueous phase containing the mixed wall material and mixing until completely dissolved to obtain a mixed aqueous phase; (3) adding the mixed microemulsion into the mixed aqueous phase, stirring at high speed and then spray drying to obtain the composite microcapsule powder.

8. The method for preparing the composite microcapsule powder according to claim 7, characterized in that: In step (1) of the preparation method, the mass ratio of the krill sea buckthorn seed mixed oil to the sea buckthorn fruit oil is (15-30):7; the emulsifier is mono- and di-glycerol fatty acid esters; the mono- and di-glycerol fatty acid esters account for 0.5-2wt% of the mass of the mixed microemulsion, the tremella polysaccharide accounts for 2-10wt% of the mass of the mixed microemulsion, and the heating temperature is 30-80°C.

9. The method for preparing the composite microcapsule powder according to claim 7, characterized in that: In step (2) of the preparation method, the mixed wall material is composed of sodium caseinate and modified starch; the sodium caseinate accounts for 1-4wt% of the mass of the mixed aqueous phase, and the modified starch accounts for 5-20wt% of the mass of the mixed aqueous phase; the mass ratio of the Hericium erinaceus polysaccharide to water is 1:(2-5); and the mixing temperature is 80°C; In step (3) of the preparation method, the mixed microemulsion is added to the mixed aqueous phase, and after high-speed shear mixing at 3000-5000 rpm for 10-20 minutes, the mixture is spray-dried at 150-180° C. to obtain the composite microcapsule powder.

10. Use of the composite microcapsule powder according to any one of claims 1 to 6, and / or the composite microcapsule powder prepared by the preparation method of the composite microcapsule powder according to any one of claims 7 to 9 in the manufacture of food, health care products and drug delivery, wherein the composite microcapsule powder can be made into powder, tablets, hard capsules and soft capsules.

Images