Traditional Chinese medicine composition with effects of warming kidney to strengthen yang and promoting blood circulation to arrest pain and preparation method of traditional Chinese medicine composition

Abstract

The invention provides a traditional Chinese medicine composition with the effects of warming kidney, supporting yang, promoting blood circulation and relieving pain. The traditional Chinese medicine composition is prepared from the following components: agaric, ligusticum wallichii, radix paeoniae rubra, radix achyranthis bidentatae, dragon's blood, cassia twig, eucommia ulmoides (charcoal), herba pyrolae, radix puerariae, rhizoma corydalis, oyster, radix salviae miltiorrhizae, herba epimedii, fructus psoraleae, trogopterus dung and honey-fried licorice root. The radix salviae miltiorrhizae, the herba epimedii, the fructus psoraleae and the trogopterus dung are added in the formula, so that the effects of nourishing blood, promoting blood circulation, dispersing blood stasis, relieving pain, strengthening muscles and bones, warming and invigorating kidney yang, and promoting blood circulation to relieve pain are improved, and the drug effect is remarkably improved by warming and invigorating, removing blood stasis, promoting blood circulation and relieving pain and blending the medicines with the honey-fried licorice root. Pharmacodynamic experiments prove that the traditional Chinese medicine composition prepared by the invention is remarkable in efficacy, high in bioavailability and stable and reliable in quality, can be prepared into different dosage forms according to the requirements of patients, and is convenient to carry and easy to take.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicines, and in particular to a traditional Chinese medicine composition having the effects of warming the kidney and invigorating yang, promoting blood circulation and relieving pain, and a preparation method thereof. Background Art

[0002] With the improvement of people's living standards, the accelerated pace of life, the increase in work pressure, and irregular diet, the pain, stiffness, numbness and other diseases caused by kidney deficiency, blood stasis, and cold obstruction are showing a clear upward trend, seriously affecting the quality of life. Most chemical drugs are chemical synthetic preparations, which have a short-term analgesic effect, but have large side effects and cannot be fundamentally treated.

[0003] The search for a Chinese medicine preparation from traditional Chinese medicine that is effective, safe, and easy to take has attracted widespread attention from the medical community at home and abroad. Summary of the invention

[0004] In view of this, the technical problem to be solved by the present invention is to provide a Chinese medicine composition with the effects of warming the kidney and invigorating yang, promoting blood circulation and relieving pain, and a preparation method thereof, which has a significant therapeutic effect on diseases such as pain, stiffness, numbness, etc. caused by kidney deficiency, blood stasis, and cold evil obstruction.

[0005] To achieve the above object, the present invention provides a Chinese medicine composition having the effects of warming the kidney and invigorating yang, promoting blood circulation and relieving pain, comprising the following components:

[0006] Fungus, Chuanxiong, Red Peony Root, Achyranthes Bidentata, Sanguisorba Officinalis, Cassia Twig, Eucommia (Charcoal), Deer Tongue Herb, Pueraria lobata, Corydalis yanhusuo, Oyster, Salvia miltiorrhiza, Epimedium, Psoralea corylifolia, Aconiti Lateralis Preparata, and Roasted Licorice.

[0007] The invention aims at the diseases such as pain, stiffness and numbness caused by kidney deficiency and blood stasis and cold evil obstruction, takes warming the kidney and assisting yang, promoting blood circulation and relieving pain as the treatment principle, adopts black fungus, chuanxiong, red peony root, Achyranthes bidentata, Sanguisorba officinalis, cinnamon twig, Eucommia bark (charcoal), deer tongue herb, kudzu root, Corydalis yanhusuo and oyster as the prescription, and adds salvia miltiorrhiza, epimedium, psoralea corylifolia and aconiti lobata. In the prescription, black fungus can nourish kidney yin and benefit essence and blood, and is the main medicine; salvia miltiorrhiza and chuanxiong are used together to promote blood circulation and remove blood stasis, promote qi circulation and remove stagnation, and enhance the effects of promoting blood circulation and removing blood stasis, relaxing muscles and relieving pain, and are the assistant medicines; red peony root clears away heat and cools blood, removes blood stasis and relieves pain, and Achyranthes bidentata, psoralea corylifolia, epimedium and Eucommia bark can both nourish kidney qi, warm kidney yang, nourish the kidney and replenish essence, and can nourish the acquired foundation, and are combined with the main medicine to nourish yin and yang, and are altogether the adjuvant medicines. Xuejie, cinnamon twig, kudzu root, and Corydalis yanhusuo can stop bleeding and relieve pain, warm the meridians and promote blood circulation. They are used together with the main medicine to help the waist and knees, replenish vital energy, replenish deficiency and fatigue, and strengthen the muscles and bones. Roasted licorice harmonizes the various medicines. The combination of all the medicines can achieve the effects of warming the kidney and supporting yang, promoting blood circulation and relieving pain.

[0008] Preferably, the Chinese medicine composition comprises the following components in parts by weight:

[0009] 8-20 parts of fungus, 8-15 parts of chuanxiong, 6-15 parts of red peony root, 6-15 parts of achyranthes bidentata, 10-15 parts of blood clotting agent, 10-15 parts of cassia twig, 4-10 parts of eucommia (charcoal), 4-10 parts of deer tongue grass, 4-8 parts of kudzu root, 4-8 parts of yanhusuo, 4-8 parts of oyster, 8-15 parts of salvia miltiorrhiza, 6-15 parts of epimedium, 6-15 parts of psoralea corylifolia, 4-8 parts of aconiti lobata, and 2-6 parts of roasted liquorice.

[0010] In a specific embodiment of the present invention, the above-mentioned Chinese medicine composition comprises the following components:

[0011] 20 parts of fungus, 15 parts of chuanxiong, 15 parts of red peony root, 15 parts of achyranthes bidentata, 15 parts of blood clotting agent, 15 parts of cassia twig, 10 parts of eucommia (charcoal), 10 parts of herba scutellariae, 8 parts of kudzu root, 8 parts of corydalis, 8 parts of oyster, 15 parts of salvia miltiorrhiza, 15 parts of epimedium, 15 parts of psoralea corylifolia, 8 parts of oliguria officinalis, and 6 parts of prepared liquorice.

[0012] In a specific embodiment of the present invention, the above-mentioned Chinese medicine composition comprises the following components:

[0013] 18 parts of fungus, 12 parts of chuanxiong, 12 parts of red peony root, 12 parts of achyranthes bidentata, 12 parts of blood clotting agent, 12 parts of cassia twig, 8 parts of eucommia (charcoal), 8 parts of deer tongue grass, 6 parts of kudzu root, 6 parts of yanhusuo, 6 parts of oyster, 12 parts of salvia miltiorrhiza, 12 parts of epimedium, 12 parts of psoralea corylifolia, 6 parts of cinnamon bark, 4 parts of prepared liquorice.

[0014] In a specific embodiment of the present invention, the above-mentioned Chinese medicine composition comprises the following components:

[0015] 16 parts of fungus, 10 parts of chuanxiong, 10 parts of red peony root, 10 parts of achyranthes bidentata, 10 parts of blood clotting agent, 10 parts of cinnamon twig, 6 parts of eucommia (charcoal), 6 parts of deer tongue grass, 4 parts of kudzu root, 4 parts of yanhusuo, 4 parts of oyster, 10 parts of salvia miltiorrhiza, 10 parts of epimedium, 10 parts of psoralea corylifolia, 4 parts of cinnamon bark, and 2 parts of roasted liquorice.

[0016] In a specific embodiment of the present invention, the above-mentioned Chinese medicine composition comprises the following components:

[0017] 14 parts of fungus, 14 parts of chuanxiong, 14 parts of red peony root, 8 parts of achyranthes bidentata, 8 parts of blood clotting agent, 8 parts of cinnamon twig, 8 parts of eucommia (charcoal), 8 parts of deer tongue grass, 6 parts of kudzu root, 6 parts of yanhusuo, 6 parts of oyster, 14 parts of salvia miltiorrhiza, 8 parts of epimedium, 8 parts of psoralea corylifolia, 6 parts of cinnamon bark, and 4 parts of prepared liquorice.

[0018] In a specific embodiment of the present invention, the above-mentioned Chinese medicine composition comprises the following components:

[0019] 10 parts of fungus, 10 parts of chuanxiong, 10 parts of red peony root, 6 parts of achyranthes bidentata, 6 parts of blood clotting, 6 parts of cinnamon twig, 4 parts of eucommia (charcoal), 4 parts of deer tongue grass, 6 parts of kudzu root, 6 parts of yanhusuo, 6 parts of oyster, 10 parts of salvia miltiorrhiza, 6 parts of epimedium, 6 parts of psoralea corylifolia, 6 parts of cinnamon bark, and 2 parts of roasted liquorice.

[0020] In a specific embodiment of the present invention, the above-mentioned Chinese medicine composition comprises the following components:

[0021] 8 parts of fungus, 8 parts of chuanxiong, 6 parts of red peony root, 6 parts of achyranthes bidentata, 6 parts of blood clotting agent, 6 parts of cinnamon twig, 6 parts of eucommia (charcoal), 4 parts of deer tongue grass, 4 parts of kudzu root, 4 parts of corydalis, 4 parts of oyster, 8 parts of salvia miltiorrhiza, 6 parts of epimedium, 6 parts of psoralea corylifolia, 4 parts of cinnamon bark, and 2 parts of roasted liquorice.

[0022] Preferably, the Rhizoma Chuanxiong and Rhizoma Cinnamomi are volatile oils obtained by steam distillation.

[0023] Preferably, the fungus, Achyranthes bidentata, Salvia miltiorrhiza, Epimedium and Psoralea corylifolia are ethanol extracts.

[0024] Preferably, the extracts of the dragon's blood, oyster, red peony root, eucommia bark (charcoal), herbaceous vine, kudzu root, corydalis, aconiti lobata and glycyrrhiza uralensis are water extracts.

[0025] The present invention enriches, separates and purifies effective parts of various medicinal materials and screens components, so that the prepared Chinese medicine composition has more precise and significant therapeutic effect.

[0026] The present invention also provides a method for preparing the above-mentioned Chinese medicine composition, comprising the following steps:

[0027] S1) crushing the raw materials of Chuanxiong and Cinnamon twig into fine powder, sieving and extracting volatile oil by steam distillation, and mixing the obtained volatile oil with cyclodextrin to obtain Chuanxiong and Cinnamon twig extracts;

[0028] S2) grinding the Auricularia auricula, Achyranthes bidentata, Salvia miltiorrhiza, Epimedium, and Psoralea corylifolia into coarse powder, and extracting with ethanol under high pressure countercurrent to obtain a first extract;

[0029] S3) extracting the blood clotting agent, oyster, red peony root, Eucommia bark (charcoal), herba scutellariae, kudzu root, corydalis, truncatum officinale, and glycyrrhiza uralensis by refluxing with water to obtain a second extract;

[0030] S4) The extract obtained in step S1) and the extracts obtained in steps S2) and S3) are mixed uniformly to obtain a Chinese medicine composition.

[0031] Preferably, the step S1) is specifically:

[0032] The raw materials of Chuanxiong and Guizhi are crushed into fine powder, sieved and then steam distilled to extract volatile oil; the volatile oil is mixed and dissolved with ethanol, mixed with β-cyclodextrin, filtered and dried to obtain the Chuanxiong and Guizhi extracts.

[0033] The particle size of the fine powder is preferably 10 to 50 mesh.

[0034] The sieving is preferably a 40 mesh sieve.

[0035] Before steam distillation extraction, it is preferred to add water in advance for soaking.

[0036] The amount of water used is preferably 8 to 12 times the mass of the original drug, and more preferably 10 times.

[0037] The soaking time is preferably 0.5 to 2 hours, more preferably 1 hour.

[0038] The steam distillation extraction time is preferably 4 to 8 hours, more preferably 6 hours.

[0039] After obtaining the volatile oil, preferably, dissolve it with an equal amount of ethanol and set aside; prepare β-cyclodextrin into a saturated aqueous solution at 45-55° C., add the volatile oil while stirring, and continue stirring at 45-55° C. for 2-4 hours, refrigerate overnight, filter, and dry to obtain Chuanxiong and Guizhi extracts.

[0040] The mass ratio of the volatile oil to β-cyclodextrin is 1:(6-12), more preferably 1:10.

[0041] Preferably, the step S2) is specifically:

[0042] The raw materials of Auricularia auricula, Achyranthes bidentata, Salvia miltiorrhiza, Epimedium and Psoralea corylifolia are crushed into coarse powder, and extracted with ethanol in high pressure countercurrent, and the ethanol is removed from the extract to obtain an ethanol extract;

[0043] The ethanol extract is mixed and diluted with water, and then purified by macroporous adsorption resin to obtain a first extract.

[0044] The particle size of the coarse powder is preferably 10 to 50 mesh.

[0045] The temperature of the ethanol high-pressure countercurrent extraction is preferably 60-80°C, more preferably 55°C.

[0046] The time for the ethanol high-pressure countercurrent extraction is preferably 30 to 90 minutes, and more preferably 30 minutes.

[0047] The mass concentration of the ethanol is preferably 30% to 75%, more preferably 60%.

[0048] The ethanol is removed from the extract, and the temperature for removing the ethanol is preferably below 50°C.

[0049] The obtained ethanol extract is mixed with water and diluted, preferably, diluted to 0.5 g of crude drug per ml.

[0050] Then it is purified by macroporous adsorption resin, preferably, first eluted with water and discarded the eluate, and then eluted with ethanol and collected the eluate.

[0051] Preferably, elution is performed with 3 column volumes of water, the eluate is discarded, and then elution is performed with 5 column volumes of 70% ethanol, the ethanol eluate is collected, and concentrated under reduced pressure at below 50°C.

[0052] Preferably, the step S3) is specifically:

[0053] Take Sanguisorba officinalis and oyster, first perform water reflux extraction, then mix with red peony root, Eucommia ulmoides (charcoal), Herba Lycopodii, Puerariae lobatae, Corydalis yanhusuo, Aconiti lobatae, Radix Glycyrrhizae and the residues of steps S1) and S2), perform water reflux extraction and then filter, the obtained filtrate is concentrated into a thick paste, which is then mixed with ethanol and allowed to stand, and the ethanol is removed from the filtrate after filtration. The obtained extract is mixed with water and diluted, and then purified by NKA-9 resin column to obtain a second extract.

[0054] The decocting time is preferably 0.5 to 2 hours, more preferably 1 hour.

[0055] The temperature of the heating reflux is preferably 100° C., and the time is preferably 90 to 120 minutes.

[0056] Preferably, the heating reflux may be performed 1 to 3 times, more preferably 2 times, and preferably each time the heating reflux is performed for 1 hour.

[0057] The relative density of the thick paste is preferably 1.10-1.20 at 60°C.

[0058] Then, the mixture is mixed with ethanol and allowed to stand. Preferably, the ethanol is 90% ethanol, so that the alcohol content in the system reaches 50%.

[0059] The standing time is preferably 12 to 36 hours, more preferably 24 hours.

[0060] After filtering, the filtrate is freed of ethanol, and the obtained extract is mixed with water and diluted. Preferably, water is added to dilute to 0.5 g of crude drug per ml.

[0061] Then, it is purified by NKA-9 resin column, preferably, first eluted with water and the eluate is discarded, and then eluted with ethanol and the eluate is collected.

[0062] More preferably, elution is performed with 4 column volumes of water, the eluate is discarded, and then elution is performed with 6 to 8 column volumes of 55% ethanol, the ethanol eluate is collected, and concentrated under reduced pressure below 50° C. to obtain a second extract.

[0063] The extract obtained in the above step S1) and the extracts obtained in steps S2) and S3) are mixed evenly to obtain a Chinese medicine composition.

[0064] The preparation method provided by the present invention adopts technical means such as ethanol high-pressure countercurrent extraction, and adopts appropriate methods to enrich, separate and purify effective parts of various medicinal materials, which can effectively improve the extraction rate of active ingredients such as auricularia auricula polysaccharide, ferulic acid, salvianolic acid B, paeoniflorin, icariin, psoralen, puerarin, etc. Experiments show that the extracts prepared by the preparation method are combined in a specific ratio to obtain a Chinese medicine composition with obvious efficacy on pain, stiffness, numbness and the like caused by kidney deficiency, blood stasis, and cold evil obstruction.

[0065] Compared with the prior art, the present invention provides a Chinese medicine composition with the effects of warming the kidney and promoting yang, promoting blood circulation and relieving pain, comprising the following components: fungus, chuanxiong, red peony root, cyathula, blood clotting, cinnamon twig, eucommia (charcoal), deer tongue grass, kudzu root, corydalis, oyster, salvia miltiorrhiza, epimedium, psoralea corylifolia, truncatum, and licorice. The addition of salvia miltiorrhiza, epimedium, psoralea corylifolia, and truncatum in the prescription helps to improve the effects of nourishing blood and promoting blood circulation, removing blood stasis and relieving pain, strengthening tendons and bones, warming and tonifying kidney yang, and promoting blood circulation and relieving pain. The medicinal effect is significantly improved by warming and tonifying, removing blood stasis, promoting blood circulation and relieving pain, and licorice harmonizes various medicines.

[0066] The Chinese medicine composition prepared by the present invention has significant efficacy, high bioavailability, stable and reliable quality, and can be prepared into different dosage forms according to the needs of patients through pharmacodynamic experiments. It is convenient to carry and easy to take. Through pharmacodynamic comparative studies, it is determined that the effect of the Chinese medicine composition of the present invention is significantly better than that of the control group. The Chinese medicine composition has significant therapeutic effects on pain, stiffness, numbness and other diseases caused by kidney deficiency and blood stasis, cold evil obstruction, and the efficacy is significantly better than that of the control group. DETAILED DESCRIPTION

[0067] In order to further illustrate the present invention, the Chinese medicine composition with the effects of warming the kidney and invigorating yang, promoting blood circulation and relieving pain provided by the present invention and its preparation method are described in detail below in conjunction with the examples. However, it should be understood that these descriptions are only for further illustrating the features and advantages of the present invention, rather than limiting the claims of the invention.

[0068] All raw materials of the present invention have no particular limitation on their sources, and can be purchased from the market or prepared according to conventional methods known to those skilled in the art.

[0069] Example 1

[0070] Ingredients: 20 parts of fungus, 15 parts of Chuanxiong, 15 parts of Danshen, 15 parts of red peony root, 15 parts of Achyranthes, 15 parts of Epimedium, 15 parts of Psoralea corylifolia, 15 parts of Sanguisorba officinalis, 15 parts of cinnamon twig, 10 parts of Eucommia (charcoal), 10 parts of Deer tongue grass, 8 parts of Pueraria root, 8 parts of Corydalis, 8 parts of Penis Enlargement Oil, 8 parts of Oyster, 6 parts of roasted Licorice root

[0071] (1) Crush Chuanxiong and cinnamon twig into fine powder, pass through a 40-mesh sieve, add 10 times the amount of water, soak for 1 hour, extract volatile oil by steam distillation for 6 hours, collect the volatile oil, dissolve it in an equal amount of ethanol, and set aside; weigh 8 times the amount of cyclodextrin of the volatile oil to prepare a saturated aqueous solution at 45-55°C, add the volatile oil while stirring, and continue stirring at 45-55°C for 3 hours, refrigerate overnight, filter, and dry.

[0072] (2) Auricularia auricula, Achyranthes bidentata, Salvia miltiorrhiza, Epimedium, and Psoralea corylifolia are ground into coarse powder, extracted with 60% ethanol in a high-pressure countercurrent stage for 30 minutes at an extraction temperature of 55°C. The drug residue after extraction is reserved for use, and ethanol is recovered below 50°C. The obtained extract is diluted with water to 0.5 g of crude drug per ml, passed through the treated macroporous adsorption resin, eluted with 3 column volumes of water, discarded the eluate, and then eluted with 70% ethanol at 5 column volumes, collected the ethanol eluate, and concentrated under reduced pressure below 50°C to obtain the product.

[0073] (3) Take Sanguisorba officinalis and oyster shells and decocted them for 1 hour, then add red peony root, Eucommia bark (charcoal), Herba Lycopodii, Radix Puerariae, Rhizoma Corydalis, Rhizoma Cibotii, Radix Glycyrrhizae and the residues of step (1) and (2), add water and reflux to extract twice, each time for 1 hour, filter, concentrate the filtrate to a thick paste with a relative density of 1.10-1.20 and a temperature of 60°C, add 90% ethanol to make the alcohol content reach 50%, let stand for 24 hours, filter, and recover ethanol from the filtrate. Dilute the obtained extract with water to 0.5 g of crude drug per milliliter, pass through the treated NKA-9 resin, elute with 4 times the column volume of water, discard the eluate, and then elute with 6-8 times the column volume of 55% ethanol, collect the ethanol eluate, and concentrate under reduced pressure below 50°C to obtain the product.

[0074] Step (4) The extracts obtained in step (1), step (2) and step (3) are mixed evenly and dried.

[0075] Example 2

[0076] Formula: Fungus 18 parts, Chuanxiong 12 parts, Danshen 12 parts, Red Peony 12 parts, Achyranthes 12 parts, Epimedium 12 parts, Psoralea corylifolia 12 parts, Sanguisorba officinalis 12 parts, Cinnamon twig 12 parts, Eucommia ulmoides (charcoal) 8 parts, Deer tongue grass 8 parts, Pueraria root 6 parts, Corydalis 6 parts, Penisula 6 parts, Oyster 6 parts, Roasted Licorice 4 parts

[0077] (1) Crush Chuanxiong and cinnamon twig into fine powder, pass through a 40-mesh sieve, add 10 times the amount of water, soak for 1 hour, extract volatile oil by steam distillation for 6 hours, collect the volatile oil, dissolve it in an equal amount of ethanol, and set aside; weigh 8 times the amount of cyclodextrin of the volatile oil to prepare a saturated aqueous solution at 45-55°C, add the volatile oil while stirring, and continue stirring at 45-55°C for 3 hours, refrigerate overnight, filter, and dry.

[0078] (2) Auricularia auricula, Achyranthes bidentata, Salvia miltiorrhiza, Epimedium, and Psoralea corylifolia are ground into coarse powder, extracted with 60% ethanol in a high-pressure countercurrent stage for 30 minutes at an extraction temperature of 55°C. The drug residue after extraction is reserved for use, and ethanol is recovered below 50°C. The obtained extract is diluted with water to 0.5 g of crude drug per ml, passed through the treated macroporous adsorption resin, eluted with 3 column volumes of water, discarded the eluate, and then eluted with 70% ethanol at 5 column volumes, collected the ethanol eluate, and concentrated under reduced pressure below 50°C to obtain the product.

[0079] (3) Take the blood dragon and oyster and decocted them for 1 hour, then add red peony root, Eucommia bark (charcoal), deer tongue grass, kudzu root, Corydalis yanhusuo, aconiti lobata, licorice root and the residues of step (1) and (2), heat and reflux to extract twice, each time for 1 hour, filter, concentrate the filtrate to a thick paste with a relative density of 1.10-1.20 and a temperature of 60°C, add 90% ethanol to make the alcohol content reach 50%, let it stand for 24 hours, filter, and recover ethanol from the filtrate. Dilute the obtained extract with water to 0.5g of crude drug per milliliter, pass it through the treated NKA-9 resin, elute with 4 times the column volume of water, discard the eluate, and then elute with 6-8 times the column volume of 55% ethanol, collect the ethanol eluate, and concentrate under reduced pressure below 50°C to obtain the product.

[0080] Step (4) The extracts obtained in step (1), step (2) and step (3) are mixed evenly and dried.

[0081] Example 3

[0082] Formula: Fungus 16 parts, Chuanxiong 10 parts, Danshen 10 parts, Red Peony 10 parts, Achyranthes 10 parts, Epimedium 10 parts, Psoralea corylifolia 10 parts, Sanguisorba officinalis 10 parts, Cinnamon twig 10 parts, Eucommia ulmoides (charcoal) 6 parts, Deer tongue grass 6 parts, Pueraria root 4 parts, Corydalis 4 parts, Penisula 4 parts, Oyster 4 parts, Roasted Licorice 2 parts

[0083] (1) Crush Chuanxiong and cinnamon twig into fine powder, pass through a 40-mesh sieve, add 10 times the amount of water, soak for 1 hour, extract volatile oil by steam distillation for 6 hours, collect the volatile oil, dissolve it in an equal amount of ethanol, and set aside; weigh 8 times the amount of cyclodextrin of the volatile oil to prepare a saturated aqueous solution at 45-55°C, add the volatile oil while stirring, and continue stirring at 45-55°C for 3 hours, refrigerate overnight, filter, and dry.

[0084] (2) Auricularia auricula, Achyranthes bidentata, Salvia miltiorrhiza, Epimedium, and Psoralea corylifolia are ground into coarse powder, extracted with 60% ethanol in a high-pressure countercurrent stage for 30 minutes at an extraction temperature of 55°C. The drug residue after extraction is reserved for use, and ethanol is recovered below 50°C. The obtained extract is diluted with water to 0.5 g of crude drug per ml, passed through the treated macroporous adsorption resin, eluted with 3 column volumes of water, discarded the eluate, and then eluted with 70% ethanol at 5 column volumes, collected the ethanol eluate, and concentrated under reduced pressure below 50°C to obtain the product.

[0085] (3) Take the blood dragon and oyster and decocted them for 1 hour, then add red peony root, Eucommia bark (charcoal), deer tongue grass, kudzu root, Corydalis yanhusuo, aconiti lobata, licorice root and the residues of step (1) and (2), heat and reflux to extract twice, each time for 1 hour, filter, concentrate the filtrate to a thick paste with a relative density of 1.10-1.20 and a temperature of 60°C, add 90% ethanol to make the alcohol content reach 50%, let it stand for 24 hours, filter, and recover ethanol from the filtrate. Dilute the obtained extract with water to 0.5g of crude drug per milliliter, pass it through the treated NKA-9 resin, elute with 4 times the column volume of water, discard the eluate, and then elute with 6-8 times the column volume of 55% ethanol, collect the ethanol eluate, and concentrate under reduced pressure below 50°C to obtain the product.

[0086] Step (4) The extracts obtained in step (1), step (2) and step (3) are mixed evenly and dried.

[0087] Example 4

[0088] Ingredients: 14 parts of fungus, 14 parts of Chuanxiong, 14 parts of Danshen, 14 parts of red peony root, 8 parts of Achyranthes, 8 parts of Epimedium, 8 parts of Psoralea corylifolia, 8 parts of Sanguisorba officinalis, 8 parts of Cassia twig, 8 parts of Eucommia (charcoal), 8 parts of Deer tongue grass, 6 parts of Pueraria root, 6 parts of Corydalis, 6 parts of Penis Enlargement Oil, 6 parts of Oyster, 4 parts of roasted Licorice root

[0089] (1) Crush Chuanxiong and cinnamon twig into fine powder, pass through a 40-mesh sieve, add 10 times the amount of water, soak for 1 hour, extract volatile oil by steam distillation for 6 hours, collect the volatile oil, dissolve it in an equal amount of ethanol, and set aside; weigh 8 times the amount of cyclodextrin of the volatile oil to prepare a saturated aqueous solution at 45-55°C, add the volatile oil while stirring, and continue stirring at 45-55°C for 3 hours, refrigerate overnight, filter, and dry.

[0090] (2) Auricularia auricula, Achyranthes bidentata, Salvia miltiorrhiza, Epimedium, and Psoralea corylifolia are ground into coarse powder, extracted with 60% ethanol in a high-pressure countercurrent stage for 30 minutes at an extraction temperature of 55°C. The drug residue after extraction is reserved for use, and ethanol is recovered below 50°C. The obtained extract is diluted with water to 0.5 g of crude drug per ml, passed through the treated macroporous adsorption resin, eluted with 3 column volumes of water, discarded the eluate, and then eluted with 70% ethanol at 5 column volumes, collected the ethanol eluate, and concentrated under reduced pressure below 50°C to obtain the product.

[0091] (3) Take the blood dragon and oyster and decocted them for 1 hour, then add red peony root, Eucommia bark (charcoal), deer tongue grass, kudzu root, Corydalis yanhusuo, aconiti lobata, licorice root and the residues of step (1) and (2), heat and reflux to extract twice, each time for 1 hour, filter, concentrate the filtrate to a thick paste with a relative density of 1.10-1.20 and a temperature of 60°C, add 90% ethanol to make the alcohol content reach 50%, let it stand for 24 hours, filter, and recover ethanol from the filtrate. Dilute the obtained extract with water to 0.5g of crude drug per milliliter, pass it through the treated NKA-9 resin, elute with 4 times the column volume of water, discard the eluate, and then elute with 6-8 times the column volume of 55% ethanol, collect the ethanol eluate, and concentrate under reduced pressure below 50°C to obtain the product.

[0092] Step (4) The extracts obtained in step (1), step (2) and step (3) are mixed evenly and dried.

[0093] Example 5

[0094] Ingredients: 10 parts of fungus, 10 parts of Chuanxiong, 10 parts of Danshen, 10 parts of red peony root, 6 parts of Achyranthes, 6 parts of Epimedium, 6 parts of Psoralea corylifolia, 6 parts of Sanguisorba officinalis, 6 parts of Cassia twig, 4 parts of Eucommia (charcoal), 4 parts of Deer tongue grass, 6 parts of Pueraria root, 6 parts of Corydalis, 6 parts of Penis Enlargement Oil, 6 parts of Oyster, 2 parts of roasted Licorice root

[0095] (1) Crush Chuanxiong and cinnamon twig into fine powder, pass through a 40-mesh sieve, add 10 times the amount of water, soak for 1 hour, extract volatile oil by steam distillation for 6 hours, collect the volatile oil, dissolve it in an equal amount of ethanol, and set aside; weigh 8 times the amount of cyclodextrin of the volatile oil to prepare a saturated aqueous solution at 45-55°C, add the volatile oil while stirring, and continue stirring at 45-55°C for 3 hours, refrigerate overnight, filter, and dry.

[0096] (2) Auricularia auricula, Achyranthes bidentata, Salvia miltiorrhiza, Epimedium, and Psoralea corylifolia are ground into coarse powder, extracted with 60% ethanol in a high-pressure countercurrent stage for 30 minutes at an extraction temperature of 55°C. The drug residue after extraction is reserved for use, and ethanol is recovered below 50°C. The obtained extract is diluted with water to 0.5 g of crude drug per ml, passed through the treated macroporous adsorption resin, eluted with 3 column volumes of water, discarded the eluate, and then eluted with 70% ethanol at 5 column volumes, collected the ethanol eluate, and concentrated under reduced pressure below 50°C to obtain the product.

[0097] (3) Take the blood dragon and oyster and decocted them for 1 hour, then add red peony root, Eucommia bark (charcoal), deer tongue grass, kudzu root, Corydalis yanhusuo, aconiti lobata, licorice root and the residues of step (1) and (2), heat and reflux to extract twice, each time for 1 hour, filter, concentrate the filtrate to a thick paste with a relative density of 1.10-1.20 and a temperature of 60°C, add 90% ethanol to make the alcohol content reach 50%, let it stand for 24 hours, filter, and recover ethanol from the filtrate. Dilute the obtained extract with water to 0.5g of crude drug per milliliter, pass it through the treated NKA-9 resin, elute with 4 times the column volume of water, discard the eluate, and then elute with 6-8 times the column volume of 55% ethanol, collect the ethanol eluate, and concentrate under reduced pressure below 50°C to obtain the product.

[0098] Step (4) The extracts obtained in step (1), step (2) and step (3) are mixed evenly and dried.

[0099] Example 6

[0100] Formula: Fungus 8 parts, Chuanxiong 8 parts, Danshen 8 parts, Red Peony 6 parts, Achyranthes 6 parts, Epimedium 6 parts, Psoralea corylifolia 6 parts, Sanguisorba officinalis 6 parts, Cinnamon twig 6 parts, Eucommia ulmoides (charcoal) 6 parts, Deer tongue grass 4 parts, Pueraria root 4 parts, Corydalis 4 parts, Penis 4 parts, Oyster 4 parts, Roasted Licorice 2 parts

[0101] (1) Crush Chuanxiong and cinnamon twig into fine powder, pass through a 40-mesh sieve, add 10 times the amount of water, soak for 1 hour, extract volatile oil by steam distillation for 6 hours, collect the volatile oil, dissolve it in an equal amount of ethanol, and set aside; weigh 8 times the amount of cyclodextrin of the volatile oil to prepare a saturated aqueous solution at 45-55°C, add the volatile oil while stirring, and continue stirring at 45-55°C for 3 hours, refrigerate overnight, filter, and dry.

[0102] (2) Auricularia auricula, Achyranthes bidentata, Salvia miltiorrhiza, Epimedium, and Psoralea corylifolia are ground into coarse powder, extracted with 60% ethanol in a high-pressure countercurrent stage for 30 minutes at an extraction temperature of 55°C. The drug residue after extraction is reserved for use, and ethanol is recovered below 50°C. The obtained extract is diluted with water to 0.5 g of crude drug per ml, passed through the treated macroporous adsorption resin, eluted with 3 column volumes of water, discarded the eluate, and then eluted with 70% ethanol at 5 column volumes, collected the ethanol eluate, and concentrated under reduced pressure below 50°C to obtain the product.

[0103] (3) Take the blood dragon and oyster and decocted them for 1 hour, then add red peony root, Eucommia bark (charcoal), deer tongue grass, kudzu root, Corydalis yanhusuo, aconiti lobata, licorice root and the residues of step (1) and (2), heat and reflux to extract twice, each time for 1 hour, filter, concentrate the filtrate to a thick paste with a relative density of 1.10-1.20 and a temperature of 60°C, add 90% ethanol to make the alcohol content reach 50%, let it stand for 24 hours, filter, and recover ethanol from the filtrate. Dilute the obtained extract with water to 0.5g of crude drug per milliliter, pass it through the treated NKA-9 resin, elute with 4 times the column volume of water, discard the eluate, and then elute with 6-8 times the column volume of 55% ethanol, collect the ethanol eluate, and concentrate under reduced pressure below 50°C to obtain the product.

[0104] Step (4) The extracts obtained in step (1), step (2) and step (3) are mixed evenly and dried.

[0105] Comparative Example 1 (replacing Salvia miltiorrhiza and Epimedium with Panax notoginseng and Cistanche deserticola)

[0106] Ingredients: 20 parts of fungus, 15 parts of Chuanxiong, 15 parts of Panax notoginseng, 15 parts of red peony root, 15 parts of Achyranthes bidentata, 15 parts of Cistanche deserticola, 15 parts of Psoralea corylifolia, 15 parts of Sanguisorba officinalis, 15 parts of cinnamon twig, 10 parts of Eucommia ulmoides (charcoal), 10 parts of Herba Lucidae, 8 parts of Puerariae, 8 parts of Corydalis, 8 parts of Penis Enlargement Oil, 8 parts of Oyster, 6 parts of roasted Licorice root

[0107] (1) Crush Chuanxiong and cinnamon twig into fine powder, pass through a 40-mesh sieve, add 10 times the amount of water, soak for 1 hour, extract volatile oil by steam distillation for 6 hours, collect the volatile oil, dissolve it in an equal amount of ethanol, and set aside; weigh 8 times the amount of cyclodextrin of the volatile oil to prepare a saturated aqueous solution at 45-55°C, add the volatile oil while stirring, and continue stirring at 45-55°C for 3 hours, refrigerate overnight, filter, and dry.

[0108] (2) Auricularia auricula, Achyranthes bidentata, Panax notoginseng, Cistanche deserticola, and Psoralea corylifolia are crushed into coarse powder, and extracted with 60% ethanol in a high-pressure countercurrent stage for 30 minutes at an extraction temperature of 55°C. The drug residue after extraction is reserved for use, and ethanol is recovered below 50°C. The obtained extract is diluted with water to 0.5 g of crude drug per ml, passed through the treated macroporous adsorption resin, eluted with 3 column volumes of water, discarded the eluate, and then eluted with 70% ethanol at 5 column volumes, collected the ethanol eluate, and concentrated under reduced pressure below 50°C to obtain the product.

[0109] (3) Take the blood dragon and oyster and decocted them for 1 hour, then add red peony root, Eucommia bark (charcoal), deer tongue grass, kudzu root, Corydalis yanhusuo, aconiti lobata, licorice root and the residues of step (1) and (2), heat and reflux to extract twice, each time for 1 hour, filter, concentrate the filtrate to a thick paste with a relative density of 1.10-1.20 and a temperature of 60°C, add 90% ethanol to make the alcohol content reach 50%, let it stand for 24 hours, filter, and recover ethanol from the filtrate. Dilute the obtained extract with water to 0.5g of crude drug per milliliter, pass it through the treated NKA-9 resin, elute with 4 times the column volume of water, discard the eluate, and then elute with 6-8 times the column volume of 55% ethanol, collect the ethanol eluate, and concentrate under reduced pressure below 50°C to obtain the product.

[0110] Step (4) The extracts obtained in step (1), step (2) and step (3) are mixed evenly and dried.

[0111] Comparative Example 2 (the prescription of Danshen, Epimedium, Psoralea corylifolia, Ophiopogon japonicus, and Radix Glycyrrhizae uralensis is missing)

[0112] Ingredients: 20 parts of black fungus, 15 parts of Chuanxiong, 15 parts of red peony root, 15 parts of Achyranthes bidentata, 15 parts of Sanguisorba officinalis, 15 parts of cinnamon twig, 10 parts of Eucommia ulmoides (charcoal), 10 parts of deer tongue grass, 8 parts of Pueraria root, 8 parts of Corydalis, 8 parts of oyster

[0113] (1) Crush Chuanxiong into fine powder, pass through a 40-mesh sieve, add 10 times the amount of water, soak for 1 hour, extract volatile oil by steam distillation for 6 hours, collect the volatile oil, dissolve it in an equal amount of ethanol, and set aside; weigh 8 times the amount of cyclodextrin of the volatile oil to make a saturated aqueous solution at 45-55°C, add the volatile oil while stirring, and continue stirring at 45-55°C for 3 hours, refrigerate overnight, filter, and dry.

[0114] (2) Take the dried blood and decocted it for 1 hour, then add red peony root, Eucommia ulmoides (charcoal), Herba Lycopodii, Rhizoma Corydalis and the residue of step (1), heat and reflux to extract twice, each time for 1 hour, filter, concentrate the filtrate to a thick paste with a relative density of 1.10-1.20 and a temperature of 60°C, add 90% ethanol to make the alcohol content reach 50%, let it stand for 24 hours, filter, and recover ethanol from the filtrate. Dilute the obtained extract with water to 0.5g of crude drug per milliliter, pass it through the treated NKA-9 resin, elute it with 4 times the column volume of water, discard the eluate, and then elute it with 6-8 times the column volume of 55% ethanol, collect the ethanol eluate, and concentrate it under reduced pressure below 50°C to obtain the product.

[0115] Step (3) The extracts obtained in step (1) and step (2) are mixed evenly and dried.

[0116] Comparative Example 3

[0117] Recipe: 500 parts of fungus, 50 parts of Chuanxiong, 50 parts of red peony root, 50 parts of Achyranthes bidentata, 50 parts of Sanguisorba officinalis, 50 parts of cinnamon twig, 50 parts of Eucommia (charcoal), 50 parts of deer tongue grass, 50 parts of Pueraria root, 67 parts of Corydalis yanhusuo, and 67 parts of oyster.

[0118] Preparation method: Dragon's blood and oyster are crushed into fine powders respectively and set aside; Auricularia auricula is respectively extracted twice with 7 times the amount of 80% ethanol and 50% ethanol by heating and refluxing, each time for 2 hours, filtered, combined with the secondary extracts, ethanol is recovered, and concentrated to a clear paste with a relative density of 1.10 (60°C) for use; The residues are decocted with 8 times the amount of water for 2 hours, filtered, combined with the above clear paste, and concentrated to a thick paste with a relative density of 1.20-1.30 (50°C); The remaining eight flavors such as Chuanxiong are crushed into coarse powders, extracted twice with 75% ethanol by heating and refluxing, the first time with 7 times the amount for 2 hours, and the second time with 3 times the amount for 1 hour, the extracts are combined, filtered, the filtrate is recovered with ethanol and concentrated to a thick paste with a relative density of 1.20-1.30 (50°C); The above two thick pastes are combined, oyster powder and starch are added in appropriate amounts, dried at 80°C, crushed into fine powder, mixed with the fine powder of Dragon's blood, and filled into capsules to prepare 1000 capsule preparations.

[0119] Comparative Example 4 (Original Wood Extract Capsule)

[0120] formula

[0121] 50 parts of black fungus, 5 parts of chuanxiong, 5 parts of red peony root, 5 parts of cyathula, 5 parts of blood clotting, 5 parts of cassia twig, 5 parts of eucommia bark (charcoal), 5 parts of kudzu root, 5 parts of yanhusuo, 6 parts of oyster, 6 parts

[0122] The above eleven herbs, including dragon's blood and oyster, are crushed into fine powders for later use; black fungus is respectively extracted twice with 7 times the amount of 80% ethanol and 50% ethanol by heating and refluxing, each time for 2 hours, filtered, the secondary extracts are combined, the ethanol is recovered, and the extracts are concentrated to a clear paste with a relative density of 1.10 (60° C.) for later use; the medicinal residues are decocted with 8 times the amount of water for 2 hours, filtered, combined with the above clear paste, and concentrated to a thick paste with a relative density of 1.20-1.30 (50° C.); the remaining eight herbs such as Chuanxiong are crushed into coarse powders, extracted twice with 75% ethanol by heating and refluxing, the first time with 7 times the amount for 2 hours, and the second time with 3 times the amount for 1 hour, the extracts are combined, filtered, the filtrate is recovered with ethanol and concentrated to a thick paste with a relative density of 1.20-1.30 (50° C.); the above two thick pastes are combined, oyster powder and starch are added in appropriate amounts, dried at 80° C., crushed into fine powder, mixed with the dragon's blood fine powder, and filled into capsules to make 1000 capsules.

[0123] Example 7 Pharmacodynamics Study

[0124] 1 Experimental Materials

[0125] 1.1 Trial Drugs

[0126] The preparations prepared in the above examples and comparative examples were provided by Jilin Xiuzhen Pharmaceutical New Drug Development Co., Ltd.

[0127] 1.2 Animals

[0128] There were 72 Wistar rats, half male and half female, and 138 ICR mice, including 36 females and 102 males, all of which were SPF grade. The animal production license number was SCXK(Ji)-2020-0002. The maintenance feed for SPF mice and rats was provided by Changchun Yisi Experimental Animal Technology Co., Ltd.

[0129] 1.3 Reagents

[0130] Interleukin-6 kit, interleukin-9 kit, testosterone (T) kit and estradiol (E2) kit were purchased from Nanjing Jiancheng Biotechnology Co., Ltd.

[0131] 2 Experimental methods:

[0132] 2.1 Therapeutic effect on rats with knee osteoarthritis of kidney deficiency and blood stasis type

[0133] 2.1.1 Grouping

[0134] 72 rats were randomly divided into a normal group of 6, a knee osteoarthritis group, and a model group of knee osteoarthritis with kidney deficiency and blood stasis (referred to as the composite model group) of 66. Methods for establishing animal models: ① The kidney deficiency model was established by ovariectomy. Ketamine (0.1g / kg) was injected intraperitoneally for anesthesia. Under aseptic operation, the rats were entered through the midline approach to the back of the waist, the fascia of the waist was separated, the ovaries were separated and exposed, and the fallopian tubes and surrounding blood vessels were ligated. The ovaries were removed and observed for 3 months. ② The blood stasis model was established by adrenocortical hormone plus epinephrine. Hydrocortisone 10mg / kg was injected intramuscularly, once a day, and epinephrine 0.36mg / kg was injected subcutaneously once after 13 days of medication. 3. The osteoarthritis model adopts the classic Hulth model, which is a classic surgical modeling method. The specific steps are: the animal is anesthetized and lies on the operating table, and a longitudinal incision of about 2 cm is made on the medial side of the knee joint under aseptic conditions to expose the knee joint, and then the anterior and posterior cruciate ligaments and the medial collateral ligament are cut off, the medial meniscus is completely removed, and the articular cartilage surface is retained. The injured limb is not fixed after surgery, and it moves freely. Antibiotics can be appropriately given to prevent infection, and the animals are driven away 7d after surgery, 30min / d, and driven away twice, and an obvious osteoarthritis model is obtained after 4 weeks. The composite model is composed of the above 3 models, and a composite osteoarthritis model is formed on the basis of kidney deficiency and blood stasis. First, a kidney deficiency model is established, and after 8 weeks, blood stasis model and osteoarthritis model are modeled simultaneously. 66 model rats are randomly divided into 11 groups, namely, a model group, a comparative example 1-4 group and an embodiment 1-6 group, 6 rats in each group, and administration is carried out for 4 weeks, and material verification is carried out after 12 weeks.

[0135] 2.1.2 Main outcome measures

[0136] 2.1.2.1 Blood rheology index detection:

[0137] After the rats were anesthetized, 5 mL of blood was collected from the abdominal aorta, anticoagulated with heparin, and shaken well. The blood rheological changes, including low shear reduction viscosity and aggregation index, were detected using a fully automatic self-cleaning blood rheometer.

[0138] 2.1.2.2 Detection of serum testosterone (T), estrogen (E2), interleukin 6 (IL-6) and interleukin 9 (IL-9)

[0139] Blood was collected from the aorta and centrifuged at 3000 r / min for 15 min to separate the serum. ELISA kits were used to measure the levels of T, E2, IL-6, and IL-9 in the serum. The specific operations were carried out according to the instructions in the kit.

[0140] 2.1.3 Experimental Results

[0141] 2.1.3.1 Changes in blood rheology in rats

[0142] The results showed that compared with the blank group, the low shear reduction blood viscosity and aggregation index of the model group increased significantly (P<0.001). Compared with the model group, the low shear reduction viscosity and aggregation index of each medication group decreased to varying degrees (P<0.05, P<0.001). Compared with the preparation group of comparative example 3, the low shear reduction viscosity and aggregation index of Example 2 and 3 groups decreased significantly to varying degrees (P<0.05, P<0.01), and the aggregation index of Example 4 group decreased significantly (P<0.05); compared with comparative example 4, the aggregation index of Example 2 decreased very significantly (P<0.01), the low shear reduction viscosity and aggregation index of Example 3 group decreased significantly (P<0.05), and the aggregation index of Example 4 group decreased significantly (P<0.05). See Table 1 for details.

[0143] Table 1 Changes of low shear reduced blood viscosity and aggregation index in rats in each group

[0144]

[0145]

[0146] Note: Compared with the blank group, △△△ P<0.001; compared with the model group, # P<0.05, ### P<0.001; compared with comparative example 3, ▲ P<0.05, ▲▲ P<0.01; compared with comparative example 4, * P<0.05,** P<0.01.

[0147] 2.1.3.2 Effects on serum levels of T, E2, IL-6, and IL-9

[0148] The results showed that compared with the blank group, the levels of IL-6, IL-9 and E2 in the serum of the model group were significantly increased (P<0.001), and the level of T was significantly decreased (P<0.001); compared with the model group, the levels of IL-6, IL-9 and E2 in the serum of each administration group were decreased to varying degrees (P<0.05, P<0.01, P<0.001), and the level of T was significantly increased (P<0.001); compared with comparative example 3, the level of IL-6 in the serum of the Example 2 group was significantly decreased (P<0.001 ), the levels of IL-6, IL-9 and E2 in serum of Example 3 group were significantly reduced (P<0.05), the level of T was significantly increased (P<0.05), and the levels of IL-6 and IL-9 in serum of Example 4 group were significantly reduced (P<0.05); compared with Comparative Example 4, the levels of IL-6, IL-9 and E2 in serum of Example 3 group were significantly reduced (P<0.05), the level of T was significantly increased (P<0.05), and the levels of IL-6 and IL-9 in serum of Example 4 group were significantly reduced (P<0.05). See Table 2 for details.

[0149] Table 2 Effect of serum IL-6 and IL-9 levels in rats in each group

[0150]

[0151]

[0152] Note: Compared with the blank group, △△△ P<0.001; compared with the model group, # P<0.05, ## P<0.01, ### P<0.001; compared with comparative example 3, ▲ P<0.05, ▲▲▲ P<0.001; compared with comparative example 4, * P<0.05.

[0153] 2.2 Experiment on pain induced by thermal stimulation in mice

[0154] 2.2.1 Experimental methods

[0155] Non-pregnant female mice with a pre-selected hot plate method pain reaction latency (pain threshold) within the range of 10-30 seconds were randomly divided into 11 groups, with 6 mice in each group. One hour after gavage on the eighth day, the mice in each group were placed on a constant temperature water bath hot plate device pre-heated to 55±0.19℃. A 1 / 100 second electronic table was used to record the time from the time the mouse was placed on the hot plate to the appearance of the paw licking reaction (latency or pain threshold period), and the percentage increase in the pain threshold of each group relative to the blank control group was calculated.

[0156] The percentage increase of pain threshold relative to the blank control group = (average pain threshold of the experimental group - average pain threshold of the blank group) / average pain threshold of the blank group

[0157] 2.2.2 Experimental results

[0158] The results showed that compared with the blank group, except for comparative examples 1 and 2, the pain thresholds of the other drug-administered groups increased to varying degrees (P<0.05, P<0.01); compared with comparative examples 3 and 4, Example 3 increased significantly (P<0.05), and the extension rate reached 92.6%. See Table 3 for details.

[0159] Table 3 Effects on the pain threshold of hot plate test in mice

[0160]

[0161]

[0162] Note: Compared with the blank group, # P<0.05, ## P<0.01, ### P<0.001; compared with the CN200610065610.5 preparation group, ▲ P<0.05; compared with Yuanmujie capsule, * P<0.05.

[0163] 2.3 Effects of acetic acid on peritoneal capillary permeability in mice

[0164] 2.3.1 Experimental methods

[0165] 72 Kunming mice, half male and half female, were randomly divided into 12 groups. Except for the first group as the normal control group, the other groups, doses, volumes and time were the same as above. One hour after the last administration, each mouse was injected with 0.5% Evans blue saline solution 0.1ml / 10g through the tail vein, and then 0.6% acetic acid 0.1ml / 10g was injected intraperitoneally (except the normal control group). After 20 minutes, the mice were killed by dislocating the cervical vertebra, the abdominal skin and muscles were cut open, and the abdominal cavity was washed several times with 6ml saline. The washing liquid was sucked out with a pipette, and then physiological saline was added to 10ml after merging. Centrifuged at 3000r / min for 15 minutes, the supernatant was taken for colorimetric determination at 590nm, and the amount of dye infiltrated into the abdominal cavity of each mouse was calculated on the regression line.

[0166] 2.3.2 Experimental Results

[0167] The results showed that each administration group could significantly inhibit the increase of peritoneal capillary permeability of mice caused by acetic acid, and had the effect of reducing capillary permeability, and the effect of Example 3 was better than that of Comparative Examples 3 and 4. See Table 4 for details.

[0168] Table 4 Effects on peritoneal capillary permeability in mice

[0169]

[0170]

[0171] Note: Compared with the blank group, △△△ P<0.001; compared with the model group, ### P<0.001; compared with the CN200610065610.5 preparation group, ▲ P<0.05; compared with Yuanmujie capsule, * P<0.05, ** P<0.01.

[0172] 3 Conclusion

[0173] Through the above-mentioned efficacy experiments conducted on the Chinese medicine composition of the present invention, it is found that the Chinese medicine composition of the present invention can reduce inflammatory reactions by inhibiting the level of inflammatory factors in serum, improve microcirculation disorders and abnormal blood rheology, improve serum sex hormone levels, and have a certain therapeutic effect on rats with kidney deficiency and blood stasis. Its effect is significantly better than that of comparative examples 1-4.

[0174] The above embodiments are only used to help understand the method and core idea of ​​the present invention. It should be noted that, for those skilled in the art, several improvements and modifications can be made to the present invention without departing from the principles of the present invention, and these improvements and modifications also fall within the scope of protection of the claims of the present invention.

Claims

1. A Chinese medicine composition having the effects of warming the kidney and invigorating yang, promoting blood circulation and relieving pain, comprising the following components: Fungus, Chuanxiong, Red Peony Root, Achyranthes Bidentata, Sanguisorba Officinalis, Cassia Twig, Eucommia (Charcoal), Deer Tongue Herb, Pueraria lobata, Corydalis yanhusuo, Oyster, Salvia miltiorrhiza, Epimedium, Psoralea corylifolia, Aconiti Lateralis Preparata, and Roasted Licorice.

2. The Chinese medicine composition according to claim 1, characterized in that: According to parts by weight, it includes the following components: 8-20 parts of fungus, 8-15 parts of chuanxiong, 6-15 parts of red peony root, 6-15 parts of achyranthes bidentata, 10-15 parts of blood clotting agent, 10-15 parts of cassia twig, 4-10 parts of eucommia (charcoal), 4-10 parts of deer tongue grass, 4-8 parts of kudzu root, 4-8 parts of yanhusuo, 4-8 parts of oyster, 8-15 parts of salvia miltiorrhiza, 6-15 parts of epimedium, 6-15 parts of psoralea corylifolia, 4-8 parts of aconiti lobata, and 2-6 parts of roasted liquorice.

3. The Chinese medicine composition according to claim 1, characterized in that According to parts by weight, it includes the following components: 20 parts of fungus, 15 parts of chuanxiong, 15 parts of red peony root, 15 parts of achyranthes bidentata, 15 parts of blood clotting, 15 parts of cinnamon twig, 10 parts of eucommia (charcoal), 10 parts of leucophylla, 8 parts of kudzu root, 8 parts of yanhusuo, 8 parts of oyster, 15 parts of salvia miltiorrhiza, 15 parts of epimedium, 15 parts of psoralea corylifolia, 8 parts of cinnamomea chinensis, 6 parts of roasted liquorice; Or 18 parts of fungus, 12 parts of chuanxiong, 12 parts of red peony root, 12 parts of achyranthes bidentata, 12 parts of blood clotting, 12 parts of cinnamon twig, 8 parts of eucommia (charcoal), 8 parts of leucophylla, 6 parts of kudzu root, 6 parts of yanhusuo, 6 parts of oyster, 12 parts of salvia miltiorrhiza, 12 parts of epimedium, 12 parts of psoralea corylifolia, 6 parts of cinnamomea chinensis, 4 parts of roasted liquorice; Or 16 parts of fungus, 10 parts of chuanxiong, 10 parts of red peony root, 10 parts of achyranthes bidentata, 10 parts of blood clotting, 10 parts of cinnamon twig, 6 parts of eucommia (charcoal), 6 parts of deer tongue grass, 4 parts of kudzu root, 4 parts of yanhusuo, 4 parts of oyster, 10 parts of salvia miltiorrhiza, 10 parts of epimedium, 10 parts of psoralea corylifolia, 4 parts of cinnamon bark, 2 parts of roasted liquorice; Or 14 parts of fungus, 14 parts of chuanxiong, 14 parts of red peony root, 8 parts of achyranthes bidentata, 8 parts of blood clotting, 8 parts of cinnamon twig, 8 parts of eucommia (charcoal), 8 parts of deer tongue grass, 6 parts of kudzu root, 6 parts of yanhusuo, 6 parts of oyster, 14 parts of salvia miltiorrhiza, 8 parts of epimedium, 8 parts of psoralea corylifolia, 6 parts of cinnamon bark, 4 parts of roasted liquorice; Or 10 parts of fungus, 10 parts of chuanxiong, 10 parts of red peony root, 6 parts of achyranthes bidentata, 6 parts of blood clotting, 6 parts of cinnamon twig, 4 parts of eucommia (charcoal), 4 parts of deer tongue grass, 6 parts of kudzu root, 6 parts of yanhusuo, 6 parts of oyster, 10 parts of salvia miltiorrhiza, 6 parts of epimedium, 6 parts of psoralea corylifolia, 6 parts of cinnamon bark, 2 parts of roasted liquorice; Or 8 parts of Auricularia auricula, 8 parts of Ligusticum chuanxiong, 6 parts of Red Peony Root, 6 parts of Achyranthes Bidentata, 6 parts of Sanguisorba Officinalis, 6 parts of Cinnamon Twig, 6 parts of Eucommia (charcoal), 4 parts of Herba Deere, 4 parts of Puerariae Radix, 4 parts of Corydalis Scutellariae, 4 parts of Oyster, 8 parts of Salvia miltiorrhiza, 6 parts of Epimedium, 6 parts of Psoralea corylifolia, 4 parts of Ophiopogon japonicus, and 2 parts of Radix Glycyrrhizae Preparata.

4. The Chinese medicine composition according to any one of claims 1 to 3, characterized in that: The rhizome of Chuanxiong and cassia twig are volatile oils obtained by steam distillation.

5. The Chinese medicine composition according to any one of claims 1 to 3, characterized in that: The fungus, Achyranthes bidentata, Salvia miltiorrhiza, Epimedium and Psoralea corylifolia are ethanol extracts.

6. The Chinese medicine composition according to any one of claims 1 to 3, characterized in that: The extracts of the dragon's blood, oyster, red peony root, eucommia bark (charcoal), herba scutellariae, kudzu root, yanhusuo, tsaoko and licorice are water extracts.

7. The method for preparing the Chinese medicine composition according to any one of claims 1 to 6, comprising the following steps: S1) crushing the raw materials of Chuanxiong and Cinnamon twig into fine powder, sieving and extracting volatile oil by steam distillation, and mixing the obtained volatile oil with cyclodextrin to obtain Chuanxiong and Cinnamon twig extracts; S2) grinding the Auricularia auricula, Achyranthes bidentata, Salvia miltiorrhiza, Epimedium, and Psoralea corylifolia into coarse powder, and extracting with ethanol under high pressure countercurrent to obtain a first extract; S3) extracting the blood clotting agent, oyster, red peony root, Eucommia bark (charcoal), herba scutellariae, kudzu root, corydalis, truncatum officinale, and glycyrrhiza uralensis by refluxing with water to obtain a second extract; S4) The extract obtained in step S1) and the extracts obtained in steps S2) and S3) are mixed evenly to obtain a Chinese medicine composition.

8. The preparation method according to claim 7, characterized in that: The step S1) is specifically as follows: The raw materials of Chuanxiong and Guizhi are crushed into fine powder, sieved and then steam distilled to extract volatile oil; the volatile oil is mixed with ethanol and dissolved, mixed with β-cyclodextrin, filtered and dried to obtain Chuanxiong and Guizhi extracts; The mass ratio of the volatile oil to beta-cyclodextrin is 1:(6-12).

9. The preparation method according to claim 7, characterized in that: The step S2) is specifically as follows: The raw materials of Auricularia auricula, Achyranthes bidentata, Salvia miltiorrhiza, Epimedium, and Psoralea corylifolia are crushed into coarse powder, and extracted with ethanol in high pressure countercurrent, and the ethanol is removed from the extract to obtain an ethanol extract; The ethanol extract is mixed and diluted with water, and then purified by a macroporous adsorption resin to obtain a first extract; The temperature of the ethanol high pressure countercurrent extraction is 60-80°C and the time is 30-90 minutes; The macroporous adsorption resin is first eluted with water and the eluent is discarded, and then eluted with ethanol and the eluent is collected.

10. The preparation method according to claim 7, characterized in that: The step S3) is specifically as follows: Take Sanguisorba officinalis and oyster, decoct them first, then mix them with red peony root, Eucommia bark (charcoal), Herba Lycopodii, Radix Puerariae, Rhizoma Corydalis, Rhizoma Aconiti Lateralis, Radix Glycyrrhizae Preparata and the residues of steps S1) and S2), heat and reflux for extraction and then filter, concentrate the obtained filtrate into a thick paste, then mix it with ethanol and let it stand, filter and remove ethanol from the filtrate, mix the obtained extract with water and dilute it, and purify it with NKA-9 resin column to obtain a second extract; The heating reflux temperature is 100°C and the time is 90 to 120 minutes; The relative density of the thick paste is 1.10-1.20 at 60°C; The purification by NKA-9 resin column specifically comprises first eluting with water and discarding the eluate, and then eluting with ethanol and collecting the eluate.

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