Compound traditional Chinese medicine preparation for resisting porcine reproductive and respiratory syndrome virus and use method thereof

Abstract

The invention provides an anti-porcine reproductive and respiratory syndrome virus compound traditional Chinese medicine preparation and a use method thereof. The compound traditional Chinese medicine preparation for resisting the porcine reproductive and respiratory syndrome virus comprises rhizoma polygonati, fried malt, liquorice, radix aconiti lateralis preparata, fructus psoraleae, rhizoma curculiginis, platycodon grandiflorum, parasitic loranthus, morinda officinalis and fructus gardeniae. When the compound traditional Chinese medicine preparation is used for preventing and treating PRRS (porcine reproductive and respiratory syndrome), the litter size, the live litter size and the feeding rate of sows can be effectively increased, the stillbirth number and the mummy birth number are reduced, the average birth weight, the uniformity and the daily gain of piglets are obviously increased, and the diarrhea rate of the piglets is reduced; the sow immunity is enhanced.

Description

Technical Field

[0001] The invention relates to the technical field of Chinese veterinary medicine preparation, and in particular to a compound Chinese medicine preparation for resisting porcine blue ear virus and a use method thereof. Background Art

[0002] Porcine reproductive and respiratory syndrome (PRRS), caused by porcine reproductive and respiratory syndrome virus (PRRSV), is a highly contagious disease that causes reproductive disorders such as loss of appetite, persistent fever, mummified fetuses, weak piglets, abortion, premature births, and other reproductive disorders in pregnant sows, as well as high mortality and respiratory diseases in suckling piglets and growing and fattening pigs. PRRSV infection can also manifest clinically as symptoms such as repeated infertility, atypical estrus, immunosuppression, and persistent infection in estrus sows. The skin ends of pigs infected with PRRSV, especially the tips of the ears, are cyanotic and purple, so most people call porcine reproductive and respiratory syndrome "blue ear disease." Vaccination in pig farms is still an important means of preventing and controlling PRRS. However, the virus is easy to mutate. There are many strains prevalent in pig farms in my country. Vaccination in many pig farms cannot provide effective protection, and PRRS often occurs.

[0003] Therefore, how to effectively prevent PRRS is particularly important. Summary of the invention

[0004] The object of the present invention is to provide a composite Chinese medicine preparation for resisting porcine blue ear virus and a method of using the same.

[0005] A compound Chinese medicine preparation for resisting porcine blue ear virus comprises: polygonatum, roasted malt, liquorice, aconite root, psoralea corylifolia, curculigo orchioides, platycodon grandiflorum, mistletoe, morinda officinalis and gardenia jasminoides.

[0006] Furthermore, the above-mentioned anti-porcine blue ear virus compound Chinese medicine preparation includes, by mass: 3-25 parts of polygonatum; 4-19 parts of roasted malt; 1-10 parts of aconite root; 6-15 parts of liquorice; 13-35 parts of psoralea corylifolia; 12-30 parts of curculigo; 4-16 parts of platycodon; 5-30 parts of mistletoe; 5-30 parts of morinda officinalis; and 0.5-15 parts of gardenia.

[0007] Furthermore, the above-mentioned anti-porcine blue ear virus compound Chinese medicine preparation includes, by mass: 14-17 parts of polygonatum; 8-11 parts of roasted malt; 5-6 parts of aconite root; 8-11 parts of liquorice; 25-30 parts of psoralea corylifolia; 25-28 parts of curculigo; 7-8 parts of platycodon; 11-14 parts of mistletoe; 11-14 parts of morinda officinalis; 3-5 parts of gardenia.

[0008] The present invention also provides a method for using the above-mentioned anti-PRRS virus compound Chinese medicine preparation, comprising: First course of treatment: Start feeding after the sows are weaned from the previous litter, and continue the medication for 14 days; Second course of treatment: 30 days after the end of the first course of treatment, continue to take the medicine for 7 days; The third course of treatment: Start feeding the sow 7 days before the next due date and continue the medication for 7 days; Fourth course of treatment: Start feeding the sows after they give birth and continue taking the medicine for 7 days.

[0009] Processed Polygonatum: tonifies Qi and nourishes Yin, strengthens the spleen, moistens the lungs, and benefits the kidneys.

[0010] Fried malt: promotes qi and aids digestion, strengthens the spleen and stimulates appetite.

[0011] Roasted licorice: nourishes the spleen and stomach, replenishes qi and restores the pulse, relieves cough and asthma, and harmonizes the properties of the medicine.

[0012] Fu Pian: restores yang and rescues adverse conditions, replenishes fire and supports yang, dispels cold and removes dampness. Treats yin excess and suppresses yang, profuse sweating and loss of yang, vomiting, diarrhea, syncope, cold pain in the heart and abdomen, spleen diarrhea and cold dysentery, beriberi, edema, slow convulsions in children, wind-cold-dampness arthralgia, carbuncle, fistula and all diseases of deep cold and chronic cold.

[0013] Psoralea corylifolia: warms the kidney and assists yang, replenishes qi and relieves asthma, warms the spleen and stops diarrhea. It has the effects of warming the spleen and stopping diarrhea, improves the function of the digestive system and reduces the occurrence of diarrhea.

[0014] Curculigo: tonifies kidney yang, strengthens tendons and bones, has sex hormone-like effects, and enhances immunity.

[0015] Platycodon: It can clear the lungs and eliminate phlegm, relieve sore throat and discharge pus, and enhance the body's anti-inflammatory and immune functions.

[0016] Mistletoe: nourishes the liver and kidneys, strengthens the bones and muscles, and stabilizes pregnancy.

[0017] Morinda officinalis: replenishes kidney yang, expels cold and dampness, and strengthens tendons and bones.

[0018] Gardenia: clears away heat and dampness, cools blood and detoxifies The invention is based on the clinical symptoms of PRRS-infected pigs and the damage of internal organs and tissues in pathological autopsy, combined with the traditional Chinese medicine theory, and adopts the main compatibility idea of ​​invigorating the spleen and replenishing qi, invigorating the liver and kidney, and strengthening the tendons and bones. The corresponding Chinese medicinal pieces of polygonatum, roasted licorice, and fried malt are selected to invigorate the spleen and stomach and replenish qi, aconite root, psoralea corylifolia, curculigo orchioides, mistletoe, and Morinda officinalis are selected to replenish the liver and kidney and strengthen the tendons and bones, platycodon grandiflorum is used to promote lung function and eliminate phlegm, and gardenia is used to clear away heat and detoxify.

[0019] In the prevention and treatment of PRRS, this compound preparation can effectively increase the number of piglets born, the number of live piglets, and the suckling rate of sows, reduce the number of stillbirths and mummified fetuses, and significantly increase the average birth weight, uniformity, and daily weight gain of piglets, and reduce the diarrhea rate of piglets; enhance the immunity of sows; and safely and effectively promote the repair of liver and kidney damage caused by PRRS in sows, providing a new technology for the safe and effective prevention and treatment of the disease. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 This is the electrophoresis diagram of swine fever in the experimental example of the present invention; wherein, the target band is 260bp, M is DL2000 Marker; + is the positive control; - is the negative control; 1 and 2 are samples; Figure 2 The pseudorabies electrophoresis diagram in the experimental example of the present invention; wherein the target band is 260 bp; M is DL2000 Marker; + is a positive control; - is a negative control; 1 and 2 are samples; Figure 3 The PRRS electrophoresis diagram in the experimental example of the present invention; the target band is 500bp; M is DL2000 Marker; + is positive control; - is negative control; 1 and 2 are samples; Figure 4 The circular electrophoresis diagram in the experimental example of the present invention; the target band is 600bp; M is DL2000 Marker; + is positive control; - is negative control; 1 and 2 are samples; Figure 5 This is the red bengal plate agglutination test in the experimental example of the present invention; Figure 6 This is a comparison chart of the number of piglets born and the number of piglets alive in the experimental examples of the present invention; Figure 7 This is a comparison chart of the number of mummies in the experimental examples of the present invention; Figure 8 This is a comparison chart of average birth weight in the experimental examples of the present invention; Fig. 9 This is a comparison chart of daily weight gain in the experimental examples of the present invention; Fig.10 This is a comparison chart of WBC changes in the experimental example of the present invention; Fig.11 A comparison diagram of HGB changes in the experimental examples of the present invention; Fig.12 This is a comparison chart of HCT changes in the experimental examples of the present invention; Fig.13 This is a comparison chart of RBC changes in the experimental examples of the present invention; Fig.14 This is a comparison chart of ALT changes in the experimental examples of the present invention; Fig.15 This is a comparison chart of AST changes in the experimental examples of the present invention; Fig.16 It is a comparison chart of CRE changes in the experimental example; Fig.17 This is a comparison chart of GLU changes in the experimental example; Fig.18 This is a comparison chart of antibody frequency bands before medication in the experimental example; Fig.19 This is a comparison chart of antibody frequency bands during the drug use period in the experimental example; Fig. 20 This is a comparison chart of antibody frequency bands after drug administration in the experimental example. DETAILED DESCRIPTION

[0021] In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be clearly and completely described below in conjunction with the drawings of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0022] There is no specific treatment for viral infectious diseases in modern Western medicine. The main measures taken are vaccination, biosafety isolation and prevention, etc., to block the source of infection and the route of transmission. There is no better way to treat susceptible animals except for very limited measures to enhance their health, such as giving them vitamins and trace elements. According to the theory and practice of traditional Chinese medicine, the rational use of many Chinese medicines, especially by enhancing the spleen and stomach function, liver and kidney function of animals to obtain sufficient nutrients and efficiently utilizing these nutrients, can effectively enhance the body's immune function, effectively prevent the attack of pathogenic microorganisms or reduce the degree of damage.

[0023] In order to effectively improve the prevention and treatment effect of PRRS in large-scale pig farms, the present invention proposes a Chinese medicine compound preparation and its use method, which can strengthen the spleen and qi, replenish liver and kidney functions, enhance the body's immunity, resist blue ear virus infection, and eliminate PRRS. It can effectively prevent and treat clinical symptoms such as cough and asthma, abortion of breeding sows, and stillbirth caused by PRRS infection, and eliminate the infection, and positive pig antigens turn negative. By using a reasonably compatible Chinese medicine compound preparation, the infection and prevalence of PRRS in large-scale pig farms can be effectively controlled.

[0024] Embodiment 1: 3 parts of polygonatum; 19 parts of roasted malt; 10 parts of aconite root; 15 parts of liquorice; 20 parts of psoralea corylifolia; 30 parts of curculigo; 4 parts of platycodon; 5 parts of parasitic loranthus; 30 parts of morinda officinalis; 0.5 parts of gardenia.

[0025] Embodiment 2: 25 parts of polygonatum; 4 parts of roasted malt; 1 part of aconite root; 6 parts of liquorice; 35 parts of psoralea corylifolia; 30 parts of curculigo; 16 parts of platycodon; 30 parts of parasitic loranthus; 5 parts of morinda officinalis; 15 parts of gardenia.

[0026] Embodiment 3: 14 parts of polygonatum; 10 parts of roasted malt; 6 parts of aconite root; 10 parts of liquorice; 13 parts of psoralea corylifolia; 12 parts of curculigo; 8 parts of platycodon; 13 parts of parasitic loranthus; 13 parts of morinda officinalis; 4 parts of gardenia.

[0027] Embodiment 4: 15 parts of polygonatum, 11 parts of roasted malt, 5 parts of aconite root, 8 parts of liquorice, 25 parts of psoralea corylifolia, 28 parts of curculigo, 7 parts of platycodon grandiflorum, 11 parts of parasitic loranthus, 14 parts of morinda officinalis, and 3 parts of gardenia.

[0028] Embodiment 5: 17 parts of polygonatum; 8 parts of roasted malt; 6 parts of aconite root; 11 parts of liquorice; 30 parts of psoralea corylifolia; 25 parts of curculigo; 8 parts of platycodon; 14 parts of parasitic loranthus; 11 parts of morinda officinalis; 5 parts of gardenia.

[0029] Experimental example: Pigs in the diseased farms were clinically diagnosed based on reproductive symptoms, respiratory symptoms, digestive symptoms, systemic symptoms, etc. (Table 1). Corresponding etiological tests were performed based on clinical symptoms and pathological changes. Comprehensive analysis was performed to confirm the diagnosis.

[0030]

[0031] 100 weaned sows were randomly selected and uniformly moved into adjacent pens for unified feeding and management. The 100 sows were randomly divided into 4 groups, with 25 sows in each group, and fed according to the ratio of Example 3, as shown in Table 2.

[0032]

[0033] One week before the experiment began, all sows in the farm were injected with PRRS vaccine.

[0034] The clinical medication experiment was divided into four courses of drug feeding. In each course of the Chinese medicine group, the prepared compound Chinese medicine was mixed with feed and fed to the experimental pigs according to the requirements of the Chinese medicine prescription and grouping. The high-dose group was fed 60 g per head per day, the medium-dose group was fed 50 g per head per day, and the low-dose group was fed 40 g per head per day. The control group used tilmicosin + tyvalocin simultaneously with the Chinese medicine group.

[0035] The clinical drug administration experiment is divided into four courses of drug administration, namely: First course of treatment: Start feeding after the sows are weaned from the previous litter, and continue the medication for 14 days; Second course of treatment: 30 days after the end of the first course of treatment, continue to take the medicine for 7 days; The third course of treatment: Start feeding the sow 7 days before the next due date and continue the medication for 7 days; Fourth course of treatment: Start feeding the sows after they give birth and continue taking the medicine for 7 days.

[0036] Clinical symptom monitoring records During the medication period, the clinical symptoms of the experimental pigs were observed and recorded, including changes in body temperature, feed intake, and mental state.

[0037] Monitoring of reproductive performance of sows The number of piglets born, number of live piglets, survival rate (survival rate = total number of surviving piglets / total number of piglets born*100%), number of stillbirths, number of mummified fetuses, average birth weight, daily weight gain, piglet diarrhea rate (piglet diarrhea rate = number of piglets with diarrhea / total number of live piglets*100%), piglet uniformity (expressed as the difference (standard deviation) in birth weight of piglets and piglets in the same group), number of rearing piglets, etc. were recorded and counted for each sow.

[0038] Sample collection and handling Blood samples were collected from the anterior vena cava of sows at three stages: before (one week after vaccine injection), during (end of the third course of treatment), and after (after all medications were administered). Four pigs were randomly selected from each group, and 3 mL of whole blood and 2 mL of anticoagulated blood were collected from each pig. The anticoagulated blood collected each time was sent for routine blood tests on the same day. After the whole blood was allowed to stand, serum was separated and stored at -20°C for the detection of biochemical indicators, viral antigens and antibodies.

[0039] Routine blood test The anticoagulated blood collected three times during the experiment was sent for testing on the same day, and clinical physiological indicators (i.e., blood routine test) were tested using a fully automatic blood cell analyzer. The total white blood cell count (WBC), lymphocytes (LYM), hemoglobin (HGB), hematocrit (HCT), total red blood cell count (RBC), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), monocytes (MONO), neutrophils (GRAN) and other indicators in the blood were measured.

[0040] Blood biochemical index detection The separated serum was tested for blood biochemical indices using a fully automatic blood biochemical analyzer, including alanine aminotransferase (ALT), aspartate aminotransferase (AST), total blood protein (TP), albumin (ALB), globulin (GLB), albumin-globulin ratio (A / G), urea nitrogen (BUN), serum creatinine (CREA), blood glucose (GLU) and other indicators.

[0041] Antigen testing The PRRSV nucleic acid was extracted from the serum samples collected three times, and the RT-PCR reaction system was configured to perform PRRSV pathogen detection. A 1% agarose gel was prepared for electrophoresis test and the results were observed, which showed negative results.

[0042] Antibody testing The sera collected three times were tested for PRRS antibodies, and the test results were statistically analyzed to calculate the antibody positivity rate, S / P value, antibody dispersion, etc.

[0043] Clinical diagnosis results Through observation of the pigs in the farm, it was found that the return rate of sows after mating was high, about 13%; in the late pregnancy, about 1% of the sows had premature births and miscarriages, about 63% of the sows gave birth to mummies, about 54% of the sows gave birth to stillborn fetuses, and the rate of weak piglets produced by sows was about 55%; some sows had constipation and vomiting. The preliminary diagnosis was that the farm had sow reproductive disorders.

[0044] Laboratory diagnosis results Two blood samples were collected from the diseased pigs in the farm for testing for classical swine fever virus, pseudorabies virus, porcine reproductive and respiratory syndrome virus, porcine circovirus, and brucellosis. The test results were positive for porcine reproductive and respiratory syndrome, and negative for classical swine fever, pseudorabies, porcine circovirus, and brucellosis (Table 3, Figures 1 to 5 ), and based on the test results, the farm was finally confirmed to be a PRRS-positive farm.

[0045]

[0046] Temperature monitoring results The statistical results of body temperature changes were as follows: During the medication period, body temperature of five pigs was measured four times at random, and the body temperature measured each time was within the normal range of pigs (Table 4).

[0047]

[0048] Appetite monitoring results: During the first stage of medication, the newly weaned sows in the four groups all had a low initial average feed intake. On the first day of medication, the feed intake of the control group was higher than that of the Chinese medicine group; the feed intake of the medium-dose group and the low-dose group began to recover on the second day of medication, and the feed intake of the high-dose group began to recover four days after medication; the average feed intake of each experimental pig group slowly increased over time, but the average feed intake decreased within two days before and after mating (Table 5).

[0049]

[0050] During the second and third phases of medication, the pigs' feed intake was normal (Tables 6 and 7).

[0051]

[0052] During the fourth phase of medication, the sows in the four groups that had just given birth ate less or not at all within two days after giving birth; on the third day after giving birth, the average feed intake of the sows in the four groups recovered to 60%; on the fifth day after giving birth, the feed intake of the sows in the control group recovered to 100%, while the feed intake of the sows in the Chinese medicine group only recovered to 80%; on the sixth day after giving birth, the feed intake of the sows in the low-dose group recovered to 100%; on the seventh day after giving birth, the feed intake of the sows in the medium-dose group recovered to 100%, but the average feed intake of the pigs in the high-dose group only recovered to 80% (Table 8).

[0053]

[0054] Mental status monitoring results During the experiment, the mental state of the pigs in the entire experimental herd was normal, without any signs of depression or hyperactivity; their movements and sleep were normal; no abnormalities were found in the visible mucous membranes; and there were no abnormalities in their breathing.

[0055] Sow reproductive performance monitoring results All data were analyzed by one-way analysis of variance in SPSS 26.

[0056] The total number of piglets born in the medium-dose group was 415, and the average number of piglets born was 16.61, which was significantly higher than the 343 and 13.72 piglets in the control group (P < 0.05); the total number of live piglets in the control group was 294, and the average number of live piglets was 11.76, which was significantly lower than that in the experimental groups (P < 0.05). Figure 6 ); The total number of stillbirths in the control group was 49, and the average number of stillbirths was 1.96, which was significantly higher than the 23 in the high-dose group (P < 0.05), and also higher than the 29 and 28 in the medium and low-dose groups; the total number of mummies in the control group was 21, and the average number of mummies was 0.84, which was significantly higher than that in each experimental group (P < 0.05). (Table 9, Figure 7 ) The average birth weights of piglets in the high, medium and low dose groups were 1.39 kg, 1.39 kg and 1.38 kg, respectively, which were significantly higher than the 1.27 kg in the control group (P < 0.05). Figure 8 The average daily weight gain of piglets in the high, medium and low dose groups was 153.35 g, 178.92 g and 158.64 g respectively, which were significantly higher than the control group's 120.83 g. ( Fig. 9 ); The number of piglets with diarrhea in the control group was 105, and the diarrhea rate was 35.71%, which was significantly higher than that in the experimental groups (P < 0.05), (Table 9).

[0057] The standard deviation of piglets at birth in the medium-dose group was the smallest at 0.13, and the uniformity of piglet birth weight was the best (Table 9). The piglets were adjusted according to the total number of live piglets, and each group of 25 sows had 348 piglets. After the piglets were weaned, the nursing rate of sows in the high-dose group was 89.9%, which was higher than 84.2% in the control group (Table 9).

[0058]

[0059] Note: Data in the same row with the same lowercase letters indicate no significant difference (P>0.05), different lowercase letters indicate significant difference (P<0.05), and different uppercase letters indicate extremely significant difference (P<0.01). The same applies below.

[0060] Blood test results All data were analyzed by one-way analysis of variance in SPSS 26.

[0061] The WBC of the TCM group continued to increase from below the lower limit of normal to the normal range, increasing from 8.7×10^9 / l, 8.5×10^9 / l, 8.1×10^9 / l to 13.0×10^9 / l, 11.8×10^9 / l, 11.3×10^9 / l, respectively. The control group decreased from 8.1×10^9 / l to 7.0×10^9 / l in the middle period of medication, and increased to 10.6×10^9 / l after medication. The HGB of the TCM group continued to increase from below the lower limit of normal to normal, increasing from 7.8 g / dl, 8.0 g / dl, 7.2 g / dl to 10.7 g / dl, 11.0 g / dl, 10.3 g / dl, respectively. The control group returned to normal after the end of medication, but decreased from 9.3 g / dl to 8.9 in the middle period of medication. g / dl; HCT in the TCM group continued to increase from below the lower limit of normal to normal, increasing from 23.4%, 22.9%, 20.4% to 33.2%, 34.3%, 32.5%, respectively; the control group returned to normal after the end of medication, but decreased from 31.6% to 25.3% in the middle period of medication; RBC in the TCM group continued to increase from below the lower limit of normal, increasing from 3.58×10^12 / l and 3.83×10^12 / l in the high and medium dose groups to the normal 5.29×10^12 / l and 5.16×10^12 / l, respectively; the control group decreased from 4.87×10^12 / l to 4.03×10^12 / l in the middle period of medication; MCV in the TCM group continued to decrease from above the upper limit of normal, decreasing from 65.3 fl, 65.4 fl, 64.9 fl to 58.0 fl, 58.1 fl, 58.6 fl, close to the upper limit of normal values, the control group was 65.1 fl before medication, decreased to 63.2 fl in the middle of medication, and increased to 64.5 fl after medication; the MCH of the Chinese medicine group continued to decrease from above the upper limit of normal values, the high-dose group decreased from 22.3 pg to the normal 20.6 pg, and the control group continued to increase, from 21.4 pg to 22.2 pg; the MCHC of the Chinese medicine group continued to decrease from above the upper limit of normal values, from 34.1 g / dL, 34.9 g / dL, 34.8 g / dL to the normal 32.7 g / dL, 32.3 g / dL, 31.7 g / dL, the control group was normal before medication at 33.8 g / dL, increased to 34.1g / dL in the middle of medication, exceeding the normal value, and returned to the normal 32.8 g / dL after medication. (Table 10, Figure 10-13 ).

[0062] Comparing the data before and after medication, after medication, the WBC value of the high-dose group increased by 49.14%, the RBC value increased by 47.77%, the MCV value decreased by 11.22%, and the MCH value decreased by 7.74%; the LYM value of the medium-dose group increased by 81.25%; the HGB value of the low-dose group increased by 42.71%, the HCT value increased by 59.07%, and the MCHC value decreased by 8.91%. The above results show that the red blood cell production and balance recovery levels of sows in the high-dose group and the low-dose group in the experimental group were significantly higher than those in the control group, and the recovery speed of the immune function level in the experimental group was also faster than that in the control group. (Table 11).

[0063]

[0064]

[0065] Note: The positive percentage in the table indicates the increase in the value after medication compared with the value before medication; the negative percentage indicates the decrease in the value after medication compared with the value before medication.

[0066] Blood biochemistry test results All data were analyzed by one-way analysis of variance in SPSS 26.

[0067] During the entire experimental period, the ALT of the Chinese medicine group continued to decrease from 44% above the upper limit of normal values, and returned to normal in the middle of the medication. After the medication, the ALT value of the medium-dose group decreased by 45.9%, and the control group was still 12.5% ​​above the upper limit of normal values ​​after the medication ended; the AST of the Chinese medicine group continued to decrease from 250% above the upper limit of normal values, and the low-dose group decreased by 72.27% after the medication and returned to normal, and the control group was still 61.76% above the upper limit of normal values ​​after the medication ended; the CRE of the Chinese medicine group continued to decrease from 69% above the upper limit of normal values, and after the medication ended, the CRE of the medium-dose group decreased by 36%, only 5% above the upper limit of normal values, and the control group was still 47% above the upper limit of normal values; the GLU of the Chinese medicine group continued to increase from 16% below the lower limit of normal values ​​to normal, and the GLU value of the low-dose group increased by 51.61%, and the control group decreased in the middle of the medication, and increased in the later period, but was still 10% below the lower limit of normal values; the TP, ALB, GLB, A / G, and UREA of the four groups all changed within the normal range. (Table 12, Figure 14-17 , Table 13).

[0068]

[0069]

[0070] Antigen test results Through testing, the results of PRRSV antigen detection were as follows: before medication, 7 of the 16 samples collected were positive, 9 were negative, and the positive rate was 43.75%. During and after medication, all 16 samples collected were negative, and the positive rate was 0%.

[0071] Antibody test results The results of antibody detection before, during and after medication are shown in Table 14. Some data were analyzed for significance by one-way analysis of variance in SPSS 26.

[0072] Before medication, the PRRSV antibody positive rate of the experimental pigs was 100%. The S / P mean values ​​of the high-dose group, medium-dose group, low-dose group and control group were 2.86, 2.84, 2.87 and 2.86, respectively. In the high-dose group, 50% of the S / P value frequency band was between 2-2.8, and 50% were strongly positive (P>2.8). In the medium-dose group, 25% of the S / P value frequency band was between 0.4-2, and 75% were strongly positive (P>2.8). In the low-dose group, 25% of the S / P value frequency band was between 2-2.8, and 50% were strongly positive (P>2.8). In the control group, 75% of the S / P value frequency band was between 2-2.8, and 25% were strongly positive (P>2.8). The dispersion was 0.20, 0.14, 0.17, and 0.17, and the difference was not significant; (Table 14, Fig.18 ).

[0073] In the middle period of medication, the PRRSV antibody positive rate of the four groups was 100%, and the average S / P values ​​were 1.99, 1.99, 1.99, and 1.74, respectively, with no significant difference. Compared with before the experiment, they decreased by 0.87, 0.85, 0.88, and 1.12, respectively. The S / P value frequency band distribution of the Chinese medicine group was: 50% between 0.4-2, 50% between 2-2.8, and the S / P value frequency band of the control group was 75% between 0.4-2, and 25% was strongly positive (P>2.8). The dispersion of the Chinese medicine group was 0.15, 0.23, and 0.24, respectively, which was significantly lower than 0.75 of the control group (P<0.05); (Table 14, Fig.19 ).

[0074] After medication, the positive rates of the Chinese medicine group were 100%, 100%, and 100%, which were significantly higher than the 25% of the control group (P < 0.05). The average S / P values ​​were 0.72, 0.69, 0.68, and 0.51, respectively, with no significant difference. Compared with before the experiment, they decreased by 2.14, 2.15, 2.19, and 2.35, respectively. The S / P value frequency band distribution of the Chinese medicine group was: 100% between 0.4-2, the S / P value frequency band of the control group was 25% between 0.4-2, and 75% was negative (P < 0.4). The discreteness of the Chinese medicine group was 0.09, 0.13, and 0.14, respectively, which was significantly lower than the 0.61 of the control group (P < 0.05). (Table 14, Fig. 20 ).

[0075]

[0076] In this experiment, the pigs in the farm had serious sow reproductive disorders. The return rate of sows after mating reached 11.2%, and there were premature births and miscarriages. The number of sows giving birth to mummies reached 76.7%, the number of sows giving birth to stillborn fetuses reached 64.3%, and the number of sows giving birth to weak piglets reached 69.2%. According to the clinical symptoms of sow reproductive disorders, blood samples of the affected pigs were collected to exclude brucellosis through tube agglutination tests, and PCR technology was used to perform differential diagnosis of swine fever virus, pseudorabies virus, porcine reproductive and respiratory syndrome virus, porcine circovirus, etc. Finally, based on the clinical symptoms and the results of laboratory pathogen detection, a comprehensive analysis confirmed that it was caused by PRRS infection.

[0077] From the perspective of traditional Chinese medicine, PRRS is described in the Yellow Emperor's Classic of Internal Medicine as "whatever is manifested inside, will be manifested outside." This means that problems with the internal organs of the body will manifest on the surface of the body. In traditional Chinese medicine theory, "the kidney governs the two yin organs," which means that problems with the kidneys will manifest in reproductive disorders such as miscarriage, estrus, dead sperm, and diarrhea; "the kidney opens to the ears," which means that problems with the kidneys will manifest in the ears; "the lungs govern breathing, and the kidneys govern the intake of air," which means that respiratory symptoms such as shallow breathing and increased frequency will occur when there are problems with the kidneys; "blue is cyan, and cyan enters the liver," which means that problems with the liver will manifest in cyan and blue on the skin, ears, and other parts.

[0078] Considering the palatability of Chinese medicine, in this experiment, the prepared Chinese medicine slices were crushed and sieved through 120 mesh, and the medicine was mixed with feed and fed according to the group. During the first stage of medication, the recovery rate of feed intake of pigs in the Chinese medicine group was lower than that of the control group, among which the feed intake recovery rate of pigs in the high-dose group was lower than that of the medium-dose group and the low-dose group. In the fourth stage of medication, the feed intake rate of pigs in the high-dose group was also the worst. It is suggested that the Chinese medicine slices should be crushed and sieved through 120 mesh and then directly mixed and fed. The feed intake of sows is closely related to the amount of addition. In practice, the screening and determination of the appropriate amount of addition is also very critical.

[0079] In this experiment, both the experimental group and the control group were sows from the same pig farm who were weaned at the same time and had similar ages and parities. The semen used for breeding was provided by the same healthy boar. Under the same feeding and management conditions, the production performance of sows in three different doses of Chinese medicine groups and the control group was compared. The number of piglets born, the number of live piglets, and the suckling rate of the three Chinese medicine groups were significantly higher than those of the control group. The number of stillbirths and mummies in the control group exceeded that of the Chinese medicine group. The total number of piglets born in the medium-dose group was 415, and the average number of piglets born was 16.61, which was significantly higher than 343 in the control group (P < 0.05); the total number of live piglets in the control group was 294, and the average number of live piglets was 11.76, which was significantly lower than that in the other groups (P < 0.05); the total number of stillbirths in the control group was 49, and the average number of stillbirths was 1.96, which was significantly higher than 23 in the high-dose group (P < 0.05); the total number of mummified fetuses in the control group was 21, and the average number of mummified fetuses was 0.84, which was significantly higher than that in the other groups (P < 0.05). After weaning of piglets, the sow nursing rate in the high-dose group was 89.9%, which was higher than 84.2% in the control group.

[0080] In this experiment, the average birth weight of piglets born by sows in each dosage group was statistically analyzed. The results showed that the average birth weight of piglets in the high-dose group and the medium-dose group was 1.39 kg, and the average birth weight of piglets in the low-dose group was 1.38 kg, which was significantly higher than 1.27 kg in the control group (P < 0.05). The piglets in the medium-dose group were the most uniform, with the standard deviation of the average birth weight of piglets being only 0.13. The average daily weight gain of piglets in the medium-dose group was 178.92 g, the average daily weight gain of piglets in the low-dose group was 158.64 g, and the average daily weight gain of piglets in the high-dose group was 153.35 g, which were all significantly higher than 120.83 g in the control group. The control group had the largest number of piglets with diarrhea, reaching 105, and the diarrhea rate of piglets was 35.71%.

[0081] In summary, the anti-PRRS virus compound Chinese medicine preparation provided by the present invention can effectively increase the number of piglets born, the number of live piglets, and the suckling rate of sows in PRRS-positive farms, and reduce the stillbirth rate and mummified fetuses; at the same time, it can also significantly increase the average birth weight and daily weight gain of piglets, significantly reduce the diarrhea rate of piglets, and improve the uniformity of piglets.

[0082] In this experiment, before medication, the number of white blood cells (WBC) and lymphocytes (LYM) in the pig herd were both 21%-56% lower than the lower limit of normal values. This shows that the pigs in the pig farm were infected with blue ear virus, and the blue ear virus replicated in the macrophages of the sows, destroying the immune organs, causing a decline in immunity, and thus immunosuppression. After the medication, the number of white blood cells in the Chinese medicine group returned to normal, and the number of white blood cells in the control group was still at a low level, lower than the normal reference value. Among them, the number of white blood cells in the high-dose group increased by 49.14% compared with before medication; the number of lymphocytes in the medium-dose group increased by 81.25% compared with before medication, which was much higher than the 23.35% increase in the control group. It shows that the anti-porcine blue ear virus compound Chinese medicine preparation provided by the present invention can effectively relieve the immunosuppression of sows caused by PRRSV infection and promote the repair of their immune function.

[0083] In this experiment, before medication, the total red blood cell count (RBC), hemoglobin (HGB), and hematocrit (HCT) of the pigs were all 20%-36% lower than the lower limit of normal values, and the mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), and mean corpuscular hemoglobin concentration (MCHC) were all 1%-17% higher than the upper limit of normal values. Folic acid requires the participation of liver cells in the body to be absorbed, utilized, and metabolized. In this experiment, the experimental pigs were infected with PRRSV, which caused liver damage to the body, resulting in a decrease in the body's absorption and utilization of folic acid, thereby causing anemia. After the medication, the total number of red blood cells in the high-dose group returned to normal, an increase of 47.77% compared with before medication; the hemoglobin, hematocrit, mean corpuscular hemoglobin concentration and other values ​​of the Chinese medicine group returned to normal ranges, among which the hemoglobin in the low-dose group increased by 42.71% compared with before medication, much higher than the 13.71% increase in the control group, and the hematocrit in the low-dose group increased by 59.07% compared with before medication, much higher than the 7.99% increase in the control group. It shows that the anti-porcine blue ear virus compound Chinese medicine preparation provided by the present invention can effectively alleviate macrocytic anemia caused by folic acid and vitamin B12 deficiency in sows, and promote the recovery of anemic sows.

[0084] In this experiment, before medication, the alanine aminotransferase of the entire experimental pig group was 50% higher than the upper limit of the normal reference value, and the ratio of aspartate aminotransferase to alanine aminotransferase was greater than 2, indicating that the experimental pigs had liver parenchymal damage. After the medication, the alanine aminotransferase of the Chinese medicine group returned to normal, among which the alanine aminotransferase of the medium-dose group decreased by 45.49% compared with before medication, which was much higher than the 23.73% of the control group; the aspartate aminotransferase of the low-dose group decreased to the normal reference value, which was 72.27% lower than before medication. The results show that when sows are infected with blue ear disease and cause substantial liver damage, the use of the anti-porcine blue ear virus compound Chinese medicine preparation provided by the present invention will not cause further damage to the liver parenchyma, but will help promote the repair of liver parenchymal damage.

[0085] Among the blood biochemical indicators of sows, serum total protein (TP), albumin (ALB), globulin (GLB), albumin-globulin ratio (A / G) and other indicators are usually used to reflect whether the synthetic function of the liver is normal. Serum total protein has the function of maintaining colloidal osmotic pressure and blood pH, eliminating toxins and immune functions; albumin has the function of maintaining osmotic pressure; globulin has immunity, and the normal value of albumin-globulin ratio varies between 1-2.5. Elevated total protein in serum is common in hemoconcentration and monoclonal immunoglobulin disease caused by excessive water loss; diseases such as nephrotic syndrome, cirrhosis, and impaired liver function can lead to decreased serum total protein. In this experiment, before and after medication, the serum total protein, albumin, globulin, and albumin-globulin ratio of the experimental pig group all changed within the normal reference value range, indicating that during the experiment, the compound Chinese medicine preparation would not damage the synthetic function of the pig liver.

[0086] Among the sow's blood biochemical indicators, urea (UREA), serum creatinine (CRE), and blood glucose (GLU) are usually used to detect whether the sow's renal function is impaired. The concentration of urea in the blood is mainly affected by the function of the kidneys and the consumption and catabolism of proteins. Increased blood urea is common in renal damage, nephritis, and excessive protein decomposition in the body; decreased blood urea is common in liver parenchyma damage, acute liver atrophy, etc. Serum creatinine is the most common indicator for judging renal function. When serum creatinine is high, it means that there is a problem with renal function. Glucose in the blood is called blood glucose. Glucose is an important source of energy for the body. Diseases such as diabetes, chronic pancreatitis, hyperadrenalism, and intracranial hemorrhage can cause pathological increases in blood glucose; pathological hypoglycemia is common in islet cell tumors, acute liver disease, adrenal insufficiency, hypothyroidism, and long-term malnutrition, which can cause pathological decreases in blood glucose. In this experiment, before medication, the urea index of the experimental pig group was normal, the serum creatinine index was 78% higher than the upper limit of normal, and the blood sugar index was 16% lower than the lower limit of normal, indicating that the experimental pig group had symptoms such as kidney damage and renal dysfunction. After medication, the blood sugar index of the Chinese medicine group returned to normal, among which the blood sugar index of the low-dose group increased by 50% compared with before medication, which was much higher than the 6% increase of the control group; the blood creatinine index of the low-dose group decreased by 36.27% compared with before medication, which was only 5% higher than the upper limit of normal. The results show that when sows are infected with PRRS and their kidney function is damaged, the use of the anti-porcine blue ear virus compound Chinese medicine preparation provided by the present invention will not aggravate the damage to the kidneys, but will help the sows to restore the function of the damaged kidneys.

[0087] In this experiment, indirect ELISA was used to detect antibodies in the blood samples collected from sows. The results showed that before medication, the PRRSV antibody positivity rate of the experimental pig group was 100%, and the S / P averages of the high-dose group, medium-dose group, low-dose group and control group were 2.86, 2.84, 2.87 and 2.86, respectively. The S / P values ​​of the high-dose group and the control group were all greater than 2, and 75% of the S / P values ​​of the medium-dose group and the low-dose group were greater than 2, with no significant difference; in the middle of medication, the PRRSV antibody positivity rate of the four groups was 100%, and the average S / P values ​​were 1.99, 1.99, 1.99, and 1.74, respectively, with no significant difference, which was a decrease of 0.87, 0.85, 0.88, 1.12, half of the S / P values ​​of the three groups in the Chinese medicine group were between 2-2.8, and 25% of the S / P values ​​of the control group were strongly positive; after medication, the positive rates of the four groups were 100%, 100%, 100% and 25% respectively, and the average S / P values ​​were 0.72, 0.69, 0.68 and 0.51 respectively, with no significant difference, compared with before the experiment, they decreased by 2.14, 2.15, 2.19 and 2.35 respectively, the S / P values ​​of the three groups in the Chinese medicine group were between 0.4-2, and 75% of the S / P values ​​of the control group were negative. The level of porcine blue ear antibody is not the higher the better, the antibody dispersion is low, the uniformity is high, and the antibody value is best in a good area, so regular testing and scientific analysis are required, and regular antibody testing can warn of the outbreak of the corresponding disease. Comparing the three antibody discreteness of the four groups, before medication, the antibody discreteness of the four groups was 0.20, 0.14, 0.17, and 0.17, respectively. In the middle of medication, the antibody discreteness of the four groups was 0.15, 0.23, 0.24, and 0.75, respectively. After the end of medication, the antibody discreteness of the four groups was 0.09, 0.13, 0.14, and 0.61, respectively. It can be seen that in the middle and late stages of medication, the antibody discreteness of the control group is higher than that of the Chinese medicine group. It shows that the anti-porcine blue ear virus compound Chinese medicine preparation provided by the present invention helps to eliminate PRRS wild virus infection in sows, delays the attenuation of PRRSV antibody levels in sows, and helps to stabilize the antibody level at a good level.

[0088] In summary, the anti-PRRS virus compound Chinese medicine preparation provided by the present invention can effectively eliminate the immunosuppression caused by PRRS infection in sows, enhance the immunity of sows, and significantly improve the reproductive performance of sows. In the prevention and treatment of PRRS, not only is there no toxic side effect of liver and kidney damage in sows, but it can also promote the repair of liver and kidney damage in sows.

[0089] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A compound Chinese medicine preparation for resisting porcine blue ear virus, characterized in that: include: Polygonatum sibiricum, roasted malt, licorice, aconite root, psoralea corylifolia, curculigo orchioides, platycodon grandiflorum, mistletoe, morinda officinalis, gardenia jasminoides.

2. The anti-PRRS virus compound Chinese medicine preparation according to claim 1, characterized in that: The invention comprises, by weight: 3-25 parts of polygonatum; 4-19 parts of roasted malt; 1-10 parts of aconite root; 6-15 parts of liquorice; 13-35 parts of psoralea corylifolia; 12-30 parts of curculigo; 4-16 parts of platycodon; 5-30 parts of mistletoe; 5-30 parts of morinda officinalis; and 0.5-15 parts of gardenia.

3. The anti-PRRS virus compound Chinese medicine preparation according to claim 2, characterized in that: The invention comprises, by weight: 14-17 parts of polygonatum; 8-11 parts of roasted malt; 5-6 parts of aconite root; 8-11 parts of liquorice; 25-30 parts of psoralea corylifolia; 25-28 parts of curculigo; 7-8 parts of platycodon grandiflorum; 11-14 parts of mistletoe; 11-14 parts of morinda officinalis; and 3-5 parts of gardenia.

4. A method for using the anti-PRRS virus compound Chinese medicine preparation according to any one of claims 1 to 3, characterized in that: include: First course of treatment: Start feeding after the sows are weaned from the previous litter, and continue the medication for 14 days; Second course of treatment: 30 days after the end of the first course of treatment, continue to take the medicine for 7 days; The third course of treatment: Start feeding the sow 7 days before the next due date and continue the medication for 7 days; Fourth course of treatment: Start feeding the sows after they give birth and continue taking the medicine for 7 days.

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