Detection device for tolerance of pathogenic microorganisms

Abstract

The invention is applicable to the technical field of pathogenic microorganism research, and provides a pathogenic microorganism tolerance detection device which comprises a detection box for pathogenic microorganism tolerance detection, two vertical partition plates are fixedly mounted in the detection box, and a transverse partition plate is fixedly mounted between the two vertical partition plates; the space between the two vertical partition plates is divided into an upper detection chamber and a lower detection chamber by the transverse partition plate, namely a hot detection chamber and a cold detection chamber; the two first placement seats are arranged in the two detection chambers, and a plurality of annularly distributed placement grooves are formed in the first placement seats and are used for placing a plurality of culture dishes for culturing pathogenic microorganisms; wherein a drug injection mechanism is arranged on the detection box and is used for injecting a drug for detecting the tolerance of pathogenic microorganisms into the culture dish. According to the pathogenic microorganism tolerance detection device provided by the scheme, tolerance detection can be carried out at different temperatures and medicament concentrations at the same time, the time for pathogenic microorganism tolerance detection is greatly shortened, and the detection efficiency is improved.

Description

Technical Field

[0001] The invention belongs to the technical field of pathogenic microorganism research, and in particular relates to a pathogenic microorganism resistance detection device. Background Art

[0002] Pathogenic microorganisms refer to microorganisms that can invade the human body and cause infection or even infectious diseases, or pathogens. Among pathogens, bacteria and viruses are the most harmful. Pathogenic microorganisms refer to prions, fungi, bacteria, spirochetes, mycoplasmas, rickettsiae, chlamydia, and viruses. Research on pathogenic microorganisms and their resistance is of great significance for preventing and controlling diseases, guiding clinical treatment, and optimizing the use of antimicrobial drugs. Understanding the resistance mechanism of pathogenic microorganisms can guide the rational use of clinical drugs, avoid the abuse of antimicrobial drugs, and reduce the occurrence of drug resistance; by studying the resistance of pathogenic microorganisms, new antimicrobial drug targets can be explored, providing ideas and methods for the development of new antimicrobial drugs.

[0003] At present, when performing pathogen resistance detection, the existing pathogen resistance detection device usually places the sample to be tested in a culture dish, then adds drugs, and then places the culture dish in a detection box, and determines the drug resistance by observing the growth state of the microorganisms in the culture dish. However, it is difficult for the existing pathogen resistance detection device to simultaneously perform resistance detection on pathogens under different temperature conditions and different drug concentrations. It is necessary to perform resistance detection multiple times to realize the study of the effects of temperature and drug concentration on pathogens, which greatly prolongs the time used for resistance detection and reduces the detection efficiency. Summary of the invention

[0004] In order to solve the technical problem that existing devices are difficult to simultaneously evaluate the drug resistance of pathogenic microorganisms at different temperatures and drug concentrations, and often require multiple repeated tests, which is not only time-consuming but also reduces the detection efficiency, the present invention provides a pathogenic microorganism resistance detection device.

[0005] The present invention is implemented as follows: a pathogenic microorganism resistance detection device includes: a detection box for pathogenic microorganism resistance detection, two vertical partitions are fixedly installed in the detection box, and a horizontal partition is fixedly installed between the two vertical partitions, and the horizontal partition divides the space between the two vertical partitions into two upper and lower detection chambers, which are a hot detection chamber and a cold detection chamber respectively; two first placement seats are arranged in the two detection chambers, and the first placement seats are provided with a plurality of annular placement grooves for placing a plurality of culture dishes for culturing pathogenic microorganisms; wherein a drug injection mechanism is provided on the detection box for injecting a drug for detecting the resistance of pathogenic microorganisms into the culture dish; and a first transmission mechanism is provided in the detection box for causing the two first placement seats to rotate simultaneously to switch the culture dishes.

[0006] Preferably, two mounting plates are fixedly installed between the two vertical partitions for supporting the two first placement seats, and the first transmission mechanism includes: two first central axes respectively fixedly installed on the bottom of the two first placement seats, and the two first central axes are respectively rotatably connected to the two mounting plates; a first rotating rod rotatably installed on one side of one of the vertical partitions; four first synchronous pulleys respectively fixedly mounted on the bottom ends of the two first central axes and the first rotating rod, and a first synchronous belt is respectively mounted between the two first synchronous pulleys at the bottom ends of the two first central axes and the two first synchronous pulleys on the first rotating rod; wherein the first transmission mechanism is also equipped with a power driving mechanism for driving the first rotating rod in the first transmission mechanism to rotate, so as to realize the rotation of the first placement seat.

[0007] Preferably, a second placement seat is provided above the top of the detection box, and a plurality of annular placement slots are provided on the second placement seat for placing a plurality of medicine bottles containing medicines of different concentrations to provide medicines of different concentrations for pathogenic microorganism resistance detection. A second transmission mechanism is installed in the detection box to drive the second placement seat to rotate so as to switch different medicine bottles.

[0008] Preferably, the second transmission mechanism includes: a second central axis fixedly mounted on the bottom of the second placement seat, the second central axis being rotatably connected to the top of the detection box; two second synchronous pulleys respectively fixedly mounted on the bottom end of the second central axis and the first rotating rod, a second synchronous belt being mounted between the two second synchronous pulleys.

[0009] Preferably, a protective box with an open bottom is hinged on the top of the detection box, and the protective box covers the second placement seat and the medicine bottles thereon inside the protective box. A U-shaped handle is installed on one side of the protective box for opening the protective box.

[0010] Preferably, the injection mechanism includes: a micro liquid pump fixedly mounted on the inner wall of one side of the detection box and located outside the detection room, the liquid inlet end of the micro liquid pump being connected to a liquid inlet hose; a liquid inlet elbow fixedly connected to the other end of the liquid inlet hose, one end of the liquid inlet elbow being connected to a straw for drawing liquid medicine from a medicine bottle; an electric telescopic rod fixedly mounted on the protective box, the output dry bottom end of the electric telescopic rod being fixedly connected to the liquid inlet elbow for vertically downward movement to extend the bottom end of the straw into the medicine bottle; a three-way pipe fixedly connected to the liquid outlet end of the micro liquid pump, the other two ports of the three-way pipe being connected to a liquid outlet hose; two liquid outlet elbows both fixedly mounted on a vertical partition, one end of the two liquid outlet elbows being respectively connected to one end of two liquid outlet hoses; two nozzles respectively fixedly mounted at the bottom ends of the two liquid outlet elbows, the two nozzles being both horizontally arranged and having a plurality of liquid spray holes at the bottom, the nozzles being located directly above the corresponding culture dishes.

[0011] Preferably, a placing plate is provided in each of the plurality of placing grooves on the first placing seat, a placing groove for placing a culture dish is provided on the top of the placing plate, and a third transmission mechanism is provided in the detection box for driving the corresponding placing plates on the two first placing seats to rotate synchronously, so that the agent can be evenly sprayed into the entire culture dish to improve the resistance detection effect of pathogenic microorganisms, and the third transmission mechanism includes: a plurality of third central shafts respectively fixedly mounted on the bottom of the plurality of placing plates, and the plurality of third central shafts are respectively rotatably connected to the two first placing seats; a plurality of first straight shafts respectively fixedly sleeved on the bottom ends of the plurality of third central shafts Gears; two fourth central shafts respectively mounted on the two mounting plates are rotated, the top ends of the two fourth central shafts are fixedly sleeved with second spur gears, and the two second spur gears are respectively meshed with the two first spur gears; a second rotating rod mounted on the side of the vertical partition away from the first rotating rod is rotated, and two third synchronous pulleys are fixedly sleeved on the second rotating rod; two fourth synchronous pulleys respectively fixedly sleeved on the bottom ends of the two fourth central shafts, and the two fourth synchronous pulleys are respectively sleeved with third synchronous belts on the two third synchronous pulleys; wherein the power drive mechanism is also used to drive the second rotating rod to rotate so as to rotate the corresponding two storage plates.

[0012] It should be noted that the bearings used for the rotation of the first central axis, the second central axis and the fourth central axis are damping bearings, that is, the first placement seat, the second placement seat and the storage plate will not rotate at will without the action of external force.

[0013] Preferably, the power drive mechanism includes: a double-axis motor fixedly mounted on the inner wall of the bottom of the detection box; two connecting shafts respectively fixedly mounted on the two output shafts of the double-axis motor, and connecting blocks are fixedly mounted on the ends of the two connecting shafts away from each other; two rotating shafts respectively rotatably mounted on the two vertical partitions, the bottom end of the first rotating rod and one end of the corresponding rotating shaft are fixedly sleeved with a first bevel gear, and the two first bevel gears are meshed with each other; two second bevel gears respectively fixedly sleeved on the second rotating rod and one end of the other rotating shaft, and the two second bevel gears are meshed with each other; wherein a ratchet mechanism is connected between the rotating shaft and the connecting block, and the two ratchet mechanisms are symmetrically arranged, for allowing the two rotating shafts to rotate only in one direction and in reverse, that is, when the double-axis motor rotates forward, the connecting shaft can drive one rotating shaft to rotate, while the other rotating shaft does not rotate, and conversely, that is, when the double-axis motor reverses, one rotating shaft does not rotate, and the connecting shaft can drive the other rotating shaft to rotate in the reverse direction.

[0014] Preferably, a semiconductor refrigeration plate is embedded in the transverse partition, with the heat-generating side of the semiconductor refrigeration plate facing upward and the cooling side facing downward. Fans are installed on both sides of the semiconductor refrigeration plate, and a plurality of heat dissipation holes are opened on the upper mounting plate for allowing heat to pass through the heat dissipation holes to heat the thermal detection chamber.

[0015] Preferably, two temperature sensors are installed on one of the vertical partitions, and probes of the two temperature sensors extend into two detection chambers respectively.

[0016] Preferably, a plurality of germicidal lamps are fixedly mounted on the sides of the two vertical partitions close to each other. The plurality of germicidal lamps are located in the two detection chambers and are used to sterilize and disinfect the two detection chambers before performing resistance testing on pathogenic microorganisms.

[0017] Preferably, a control panel is provided in the detection box for setting parameters and controlling the operation of the equipment, and two front door panels are provided on the front side of the detection box, corresponding to two detection chambers for taking and placing culture dishes, and visual glass observation windows are provided on the two front door panels. The rear side of the detection box is set as an opening and is equipped with a rear inspection panel.

[0018] Compared with the related art, the pathogenic microorganism resistance detection device provided by the present invention has the following beneficial effects:

[0019] The pathogenic microorganism resistance detection device of the present invention exhibits a significant advantage in that it can simultaneously perform resistance detection at different temperatures and drug concentrations, a function that is crucial for the screening of new drugs and the re-evaluation of existing drugs.

[0020] The automated detection process of the present invention greatly reduces the interference of human factors and ensures the high repeatability and accuracy of the experimental results. This feature not only improves the detection efficiency, but also ensures the reliability and consistency of the experimental data, providing a solid foundation for scientific research and clinical applications. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 A schematic diagram of the three-dimensional structure of a pathogenic microorganism resistance detection device provided by the present invention;

[0022] Figure 2 A schematic diagram of the front view of a pathogenic microorganism resistance detection device provided by the present invention;

[0023] Figure 3 A schematic diagram of a front cross-sectional structure of a pathogenic microorganism resistance detection device provided by the present invention;

[0024] Figure 4 for Figure 3 An enlarged structural diagram of part A shown in FIG.

[0025] Figure 5 for Figure 3 An enlarged schematic diagram of the structure of part B shown in FIG.

[0026] Figure 6 for Figure 3 An enlarged schematic diagram of the structure of part C shown in FIG.

[0027] Figure 7 for Figure 3 An enlarged structural diagram of part D shown in FIG.

[0028] Figure 8 for Figure 3 An enlarged structural diagram of part E shown in FIG.

[0029] Fig. 9 for Figure 3 An enlarged structural diagram of part F shown in FIG.

[0030] Fig.10 for Figure 3 An enlarged structural diagram of the G portion shown in FIG.

[0031] Fig.11 for Figure 3 An enlarged structural diagram of the H portion shown in FIG.

[0032] Fig.12 for Figure 3 An enlarged structural diagram of part I shown in FIG.

[0033] Fig.13 It is a top view schematic diagram of the assembly of the first placement seat and the storage plate in the present invention;

[0034] Fig.14 It is a schematic diagram of the top view of the mounting plate located in the hot chamber of the present invention;

[0035] Fig.15 It is a side view structural schematic diagram of the connecting block in the present invention;

[0036] Fig.16 It is a rear view structural schematic diagram of the present invention;

[0037] Fig.17 It is a schematic diagram of the side cross-sectional structure inside the ratchet mechanism of the present invention.

[0038] Figure numerals: 1, detection box; 2, vertical partition; 3, horizontal partition; 4, first placement seat; 5, mounting plate; 6, first central axis; 7, first rotating rod; 8, first synchronous pulley; 9, first synchronous belt; 10, second placement seat; 11, medicine bottle; 12, second central axis; 13, second synchronous pulley; 14, second synchronous belt; 15, protection box; 16, micro liquid pump; 17, liquid inlet hose; 18, liquid inlet elbow; 19, straw; 20, electric telescopic rod; 21, three-way pipe; 22, liquid outlet hose; 23, liquid outlet elbow; 24, nozzle; 25, storage plate; 26, culture dish; 27, third central axis; 28, first A straight gear; 29, the fourth middle axis; 30, the second straight gear; 31, the second rotating rod; 32, the third synchronous pulley; 33, the fourth synchronous pulley; 34, the third synchronous belt; 35, the dual-axis motor; 36, the connecting shaft; 37, the connecting block; 38, the rotating shaft; 39, the first bevel gear; 40, the second bevel gear; 41, the ratchet mechanism; 42, the semiconductor cooling plate; 43, the fan; 44, the temperature sensor; 45, the germicidal lamp; 101, the control panel; 102, the front door panel; 103, the rear inspection panel; 201, the U-shaped handle; 411, the ratchet; 412, the driven wheel; 413, the pawl; 501, the heat dissipation hole. DETAILED DESCRIPTION

[0039] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by technicians in the technical field of this application; the terms used in the specification of the application herein are only for the purpose of describing specific embodiments and are not intended to limit this application; the terms "including" and "having" and any variations thereof in the specification of this application and the above-mentioned drawings are intended to cover non-exclusive inclusions. The terms "first", "second", etc. in the specification of this application and the above-mentioned drawings are used to distinguish different objects, not to describe a specific order.

[0040] Reference to "embodiments" herein means that a particular feature, structure, or characteristic described in conjunction with the embodiments may be included in at least one embodiment of the present application. The appearance of the phrase in various locations in the specification does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment that is mutually exclusive with other embodiments. It is explicitly and implicitly understood by those skilled in the art that the embodiments described herein may be combined with other embodiments.

[0041] The embodiment of the present invention provides a pathogenic microorganism resistance detection device, such as Figure 1-17As shown, the pathogenic microorganism resistance detection device includes: a detection box 1 for pathogenic microorganism resistance detection, wherein two vertical partitions 2 are fixedly installed in the detection box 1, and a horizontal partition 3 is fixedly installed between the two vertical partitions 2, and the horizontal partition 3 divides the space between the two vertical partitions 2 into two upper and lower detection chambers, which are a hot detection chamber and a cold detection chamber respectively; two first placement seats 4 are arranged in the two detection chambers, and the first placement seats 4 are provided with a plurality of annular placement grooves for placing a plurality of culture dishes 26 for culturing pathogenic microorganisms; wherein, a drug injection mechanism is provided on the detection box 1 for injecting a drug for detecting the resistance of pathogenic microorganisms into the culture dish 26; and a first transmission mechanism is provided in the detection box 1 for rotating the two first placement seats 4 at the same time to switch the culture dish 26.

[0042] In this embodiment, the pathogenic microorganism resistance detection device has greatly improved the detection efficiency through clever design. The two vertical partitions 2 and the horizontal partition 3 fixedly installed in the detection box 1 work together to accurately divide the space in the box into a hot detection chamber and a cold detection chamber, which provides the possibility for pathogenic microorganism resistance detection under different temperature conditions. The placement grooves distributed in an annular manner on the first placement seat 4 can carry multiple culture dishes 26 at the same time. In conjunction with the first transmission mechanism, the automatic switching of the culture dishes is realized without frequent manual operation, which significantly improves the automation level and work efficiency of the detection. The setting of the drug injection mechanism enables the drug to be accurately injected into each culture dish 26, ensuring the consistency of the detection conditions and reducing human errors. The use of the first transmission mechanism allows the two first placement seats 4 to rotate synchronously, which not only saves operation time, but also ensures the parallelism and comparability of the detection. By simultaneously performing resistance detection on pathogenic microorganisms under different temperature states and drug concentrations, the device greatly shortens the detection cycle and improves the detection efficiency.

[0043] In a further preferred embodiment of the present invention, two mounting plates 5 are fixedly installed between the two vertical partitions 2 for supporting the two first placement seats 4, and the first transmission mechanism includes: two first central shafts 6 respectively fixedly installed on the bottom of the two first placement seats 4, and the two first central shafts 6 are respectively rotatably connected to the two mounting plates 5; a first rotating rod 7 rotatably installed on one side of one of the vertical partitions 2; four first synchronous pulleys 8 respectively fixedly mounted on the bottom ends of the two first central shafts 6 and the first rotating rod 7, and a first synchronous belt 9 is respectively mounted between the two first synchronous pulleys 8 at the bottom ends of the two first central shafts 6 and the two first synchronous pulleys 8 on the first rotating rod 7; wherein the first transmission mechanism is also equipped with a power driving mechanism for driving the first rotating rod 7 in the first transmission mechanism to rotate so as to realize the rotation of the first placement seat 4.

[0044] In this embodiment, the two mounting plates 5 added between the two vertical partitions 2 not only stably support the two first placement seats 4, but also cleverly integrate the key components of the first transmission mechanism. The first central axis 6 is connected to the mounting plate 5 in rotation, which ensures the flexible rotation of the first placement seat 4. The rotation installation of the first rotating rod 7, and the four first synchronous pulleys 8 and the first synchronous belt 9 matched therewith, construct a set of efficient and precise transmission system, which realizes the stable and synchronous rotation of the first placement seat 4.

[0045] The introduction of the power drive mechanism provides a power source for the rotation of the first rotating rod 7, and then drives the two first placement seats 4 to rotate accurately through the linkage of the first synchronous pulley 8 and the first synchronous belt 9, ensuring the automatic switching of the culture dish 26. This design not only reduces the complexity of manual operation, but also significantly improves the automation level of detection, making the pathogenic microorganism resistance detection process more efficient and accurate. Through the optimization of the first transmission mechanism, the present invention further improves the detection efficiency, while ensuring the reliability of the detection results, providing strong technical support for the research on pathogenic microorganism resistance.

[0046] In a further preferred embodiment of the present invention, a second placement seat 10 is provided above the top of the detection box 1, and a plurality of annular placement grooves are provided on the second placement seat 10 for placing a plurality of medicine bottles 11 containing medicines of different concentrations to provide medicines of different concentrations for pathogenic microorganism resistance detection. A second transmission mechanism is installed in the detection box 1 to drive the second placement seat 10 to rotate so as to switch different medicine bottles 11.

[0047] In this embodiment, a second placement seat 10 is cleverly provided on the top of the detection box 1, and a plurality of storage slots distributed in a ring shape thereon are not only beautiful, but more importantly, they can efficiently carry a plurality of medicine bottles 11 containing medicines of different concentrations, providing a variety of medicine options for pathogenic microorganism resistance detection. The assembly of the second transmission mechanism realizes the automatic rotation of the second placement seat 10, and then can quickly and accurately switch to the required medicine bottle 11, ensuring the resistance detection of pathogenic microorganisms under different concentrations of medicines. This design not only greatly enriches the detection conditions, so that the tolerance of pathogenic microorganisms to medicines of different concentrations can be evaluated in the same test, but also reduces human errors through automated operation, and improves the accuracy and repeatability of the test. Through the efficient operation of the second transmission mechanism, the present invention further optimizes the detection process, shortens the detection cycle, and provides a more accurate and efficient technical means for the in-depth research on pathogenic microorganism resistance and drug development.

[0048] In a further preferred embodiment of the present invention, the second transmission mechanism includes: a second central axis 12 fixedly mounted on the bottom of the second placement seat 10, the second central axis 12 being rotatably connected to the top of the detection box 1; two second synchronous pulleys 13 respectively fixedly mounted on the bottom end of the second central axis 12 and the first rotating rod 7, a second synchronous belt 14 being mounted between the two second synchronous pulleys 13.

[0049] In this embodiment, the ingenious design of the second transmission mechanism further improves the automation level and detection efficiency of the detection device. The second central axis 12 fixedly installed at the bottom of the second placement seat 10 is connected to the top of the detection box 1 through rotation, ensuring the stable rotation of the second placement seat 10. The two second synchronous pulleys 13 are respectively fixed to the bottom end of the second central axis 12 and the first rotating rod 7, and the second placement seat 10 and the first rotating rod 7 are synchronized through the linkage of the second synchronous belt 14.

[0050] This design not only simplifies the operation process, but also allows the first placement seat 4 and the second placement seat 10 to be adjusted simultaneously with one drive, ensuring the accurate switching of the detection conditions, and greatly improving the flexibility and efficiency of the detection. The rapid switching of the medicine bottles 11 of different concentrations, combined with the automatic rotation of the culture dish 26, enables the comprehensive evaluation of the resistance performance of pathogenic microorganisms under various conditions in the same test, providing strong technical support for in-depth research on the resistance mechanism of pathogenic microorganisms and the development of antimicrobial drugs, while also significantly shortening the detection cycle and improving the accuracy and reliability of the test results.

[0051] In another embodiment of the present invention, a protective box 15 with an open bottom is hinged on the top of the detection box 1, and the protective box 15 covers the second placement seat 10 and the medicine bottle 11 thereon in the protective box 15. A U-shaped handle 201 is installed on one side of the protective box 15 for opening the protective box 15.

[0052] In this embodiment, a protective box 15 with an open bottom is cleverly hinged on the top of the test box 1. The design of the protective box 15 covers the second placement seat 10 and the medicine bottles 11 thereon, forming a relatively closed and easy-to-operate environment. The U-shaped handle 201 installed on one side of the protective box 15 provides a convenient operating experience for opening the protective box 15, making it easy for testers to quickly place or replace the medicine bottles 11. This design not only effectively protects the medicine bottles 11 from external contamination and ensures the purity of the test conditions, but also helps maintain the stability of the test environment and avoids the test results being affected by external interference.

[0053] In another embodiment of the present invention, the injection mechanism includes: a micro-liquid pump 16 fixedly mounted on the inner wall of one side of the detection box 1 and located outside the detection room, the liquid inlet end of the micro-liquid pump 16 is connected to a liquid inlet hose 17; a liquid inlet elbow 18 fixedly connected to the other end of the liquid inlet hose 17, one end of the liquid inlet elbow 18 is connected to a straw 19 for sucking liquid medicine from the medicine bottle 11; an electric telescopic rod 20 fixedly mounted on the protective box 15, the output dry bottom end of the electric telescopic rod 20 is fixedly connected to the liquid inlet elbow 18, and is used to move vertically downward. , extend the bottom end of the suction tube 19 into the medicine bottle 11; a three-way pipe 21 fixedly connected to the liquid outlet end of the micro liquid pump 16, and the other two ports of the three-way pipe 21 are connected to the liquid outlet hose 22; two liquid outlet elbows 23 are fixedly installed on a vertical partition 2, and one end of the two liquid outlet elbows 23 is respectively connected to one end of the two liquid outlet hoses 22; two nozzles 24 are respectively fixedly installed at the bottom ends of the two liquid outlet elbows 23, and the two nozzles 24 are both horizontally arranged, and a plurality of liquid spray holes are opened at the bottom, and the nozzles 24 are located directly above the corresponding culture dishes 26.

[0054] In this embodiment, the design of the injection mechanism realizes the accurate and automatic delivery of the medicine. As the core component, the micro liquid pump 16 can accurately extract the medicine in the medicine bottle 11 through the connection of the liquid inlet hose 17 and the liquid inlet elbow 18, in conjunction with the suction tube 19. The setting of the electric telescopic rod 20 ensures that the suction tube 19 can accurately extend into the medicine bottle 11 to achieve the absorption of the medicine. The configuration of the three-way pipe 21 and the liquid outlet hose 22, supplemented by the liquid outlet elbow 23 and the nozzle 24, forms a complete medicine delivery system. The nozzle 24 is horizontally arranged just above the culture dish 26, and the multiple liquid spray holes opened at its bottom ensure that the medicine can be sprinkled on the culture dish 26, thereby ensuring the consistency of the detection conditions. This series of designs not only realizes the automatic and accurate delivery of the medicine, but also greatly improves the accuracy and reproducibility of the detection, provides strong technical support for the resistance detection of pathogenic microorganisms, and ensures the reliability of the detection results and the improvement of the detection efficiency.

[0055] In a further preferred embodiment of the present invention, a plurality of placement slots on the first placement seat 4 are each provided with a placement plate 25, a placement slot for placing a culture dish 26 is provided on the top of the placement plate 25, and a third transmission mechanism is provided in the detection box 1 for driving the corresponding placement plates 25 on the two first placement seats 4 to rotate synchronously, so that the agent can be evenly sprayed into the entire culture dish 26, so as to improve the resistance detection effect of pathogenic microorganisms, and the third transmission mechanism includes: a plurality of third central shafts 27 respectively fixedly mounted on the bottom of the plurality of placement plates 25, and the plurality of third central shafts 27 are respectively rotatably connected to the two first placement seats 4; a plurality of first straight gears respectively fixedly sleeved on the bottom ends of the plurality of third central shafts 27 28; two fourth central shafts 29 respectively mounted on the two mounting plates 5 are rotated, the top ends of the two fourth central shafts 29 are fixedly sleeved with second spur gears 30, and the two second spur gears 30 are respectively meshed with the two first spur gears 28; a second rotating rod 31 mounted on the side of the vertical partition 2 away from the first rotating rod 7 is rotated, and two third synchronous pulleys 32 are fixedly sleeved on the second rotating rod 31; two fourth synchronous pulleys 33 respectively fixedly sleeved on the bottom ends of the two fourth central shafts 29, and the two fourth synchronous pulleys 33 and the two third synchronous pulleys 32 are respectively sleeved with third synchronous belts 34; wherein the power driving mechanism is also used to drive the second rotating rod 31 to rotate, so as to rotate the corresponding two storage plates 25.

[0056] In this embodiment, the multiple placement plates 25 on the first placement seat 4 provide precise positioning for the culture dish 26 through the placement grooves opened on the top thereof. The introduction of the third transmission mechanism realizes the synchronous rotation of the placement plates 25, ensures that the medicine can evenly cover the entire culture dish 26, and greatly improves the accuracy and reliability of pathogen resistance detection. The multiple third central shafts 27 are rotatably connected to the first placement seat 4, and cooperate with the first straight gear 28 to ensure the stable rotation of the placement plates 25.

[0057] The meshing of the second spur gear 30 on the two fourth central shafts 29 and the first spur gear 28 ensures the effective transmission of power, while the combination of the second rotating rod 31, the third synchronous pulley 32, the fourth synchronous pulley 33 and the third synchronous belt 34 further enhances the accuracy and stability of the transmission. The reuse of the power drive mechanism demonstrates the efficient integration of resources, realizes the rotation of the second rotating rod 31, and further ensures the accurate rotation of the storage plate 25, providing a guarantee for the uniform spraying of the medicine.

[0058] It should be noted that the bearings used for the rotation of the first central axis 6, the second central axis 12 and the fourth central axis 29 are damping bearings, that is, the first placement seat 4, the second placement seat 10 and the placement plate 25 will not rotate at will without the action of external force. The application of the damping bearing effectively prevents the first placement seat 4, the second placement seat 10 and the placement plate 25 from rotating at will without the action of external force, thereby ensuring the stability of the detection conditions.

[0059] In a further preferred embodiment of the present invention, the power drive mechanism includes: a double-axis motor 35 fixedly mounted on the inner wall of the bottom of the detection box 1; two connecting shafts 36 respectively fixedly mounted on the two output shafts of the double-axis motor 35, and the ends of the two connecting shafts 36 away from each other are fixedly mounted with connecting blocks 37; two rotating shafts 38 respectively rotatably mounted on the two vertical partitions 2, and the bottom end of the first rotating rod 7 and one end of the corresponding rotating shaft 38 are fixedly sleeved with a first bevel gear 39, and the two first bevel gears 39 are meshed; and the two connecting shafts 36 are respectively fixedly sleeved on the first and second vertical partitions 2. The two second bevel gears 40 on the second rotating rod 31 and at one end of the other rotating shaft 38 are meshed with each other; wherein a ratchet mechanism 41 is connected between the rotating shaft 38 and the connecting block 37, and the two ratchet mechanisms 41 are symmetrically arranged to enable the two rotating shafts 38 to rotate only in one direction and in the reverse direction, that is, when the dual-axis motor 35 rotates forward, the connecting shaft 36 can drive one rotating shaft 38 to rotate while the other rotating shaft does not rotate; conversely, when the dual-axis motor 35 rotates reversely, one rotating shaft does not rotate, while the connecting shaft 36 can drive the other rotating shaft 38 to rotate in the reverse direction.

[0060] In this embodiment, the ingenious design of the power drive mechanism provides a stable power source for the core operation. The dual-axis motor 35, as a power source, realizes effective power transmission through the connecting shaft 36 and the connecting block 37 on its output shaft. The fine meshing of the first bevel gear 39 and the second bevel gear 40 ensures the precise distribution and conversion of power. The innovative application of the two ratchet mechanisms 41 enables the two rotating shafts 38 to achieve directional and reverse rotation, and cooperates with the forward and reverse rotation of the dual-axis motor 35 to achieve precise control of the first rotating rod 7 and the second rotating rod 31.

[0061] This design not only realizes the effective transmission and conversion of power, but also ensures the independent and coordinated operation of the first placement seat 4 and the second placement seat 10, as well as the precise rotation of the storage plate 25 through the clever arrangement of the structure, creating conditions for the uniform spraying of the agent. Through the optimization of the power drive mechanism, the present invention realizes the high degree of automation and intelligence of the detection device, significantly improves the accuracy and efficiency of the detection, and provides a strong technical support for the in-depth study of the resistance detection of pathogenic microorganisms. The innovation and practicality of this design not only reflects the precise control of the detection conditions, but also greatly simplifies the detection process, shortens the detection cycle, and provides a solid technical guarantee for the efficient and accurate detection of the resistance of pathogenic microorganisms.

[0062] In a further preferred embodiment of the present invention, a semiconductor refrigeration plate 42 is embedded on the transverse partition 3, with the heat-generating side of the semiconductor refrigeration plate 42 facing upward and the cooling side facing downward. Fans 43 are installed on both sides of the semiconductor refrigeration plate 42, and a plurality of heat dissipation holes 501 are opened on the upper mounting plate 5 for allowing heat to pass through the heat dissipation holes 501 to heat the thermal detection chamber.

[0063] In this embodiment, the semiconductor cooling sheet 42 cleverly embedded on the diaphragm 3 becomes the key to regulating the temperature in the detection chamber. Its heat-generating side faces upward and the cooling side faces downward, and with the assistance of the fan 43, a unique temperature control system is formed. The multiple heat dissipation holes 501 on the upper mounting plate 5 ensure the effective dissipation of heat and provide the necessary heating strips for the thermal detection chamber. In addition, the cooperation between the fan 43 and the heat dissipation holes 501 enables the heat to be effectively dissipated into the thermal detection chamber, providing a suitable hotter temperature for the culture dish therein.

[0064] In a further preferred embodiment of the present invention, two temperature sensors 44 are installed on one of the vertical partitions 2, and the probes of the two temperature sensors 44 extend into two detection chambers respectively.

[0065] In this embodiment, two temperature sensors 44 are cleverly installed on the vertical partition 2, and their probes extend into two detection chambers respectively, realizing real-time monitoring of the experimental environment temperature. This design not only ensures the accurate collection of temperature parameters, but also provides key data support for temperature control during the experiment. The sensitive detection of the temperature sensor 44, combined with the precise control of the semiconductor cooling plate 42, jointly creates an ideal temperature control environment required for the experiment, greatly improving the accuracy and reliability of the experimental results.

[0066] In a further preferred embodiment of the present invention, a plurality of germicidal lamps 45 are fixedly installed on the sides of the two vertical partitions 2 close to each other. The plurality of germicidal lamps 45 are located in the two detection chambers and are used to sterilize and disinfect the two detection chambers before performing resistance testing on pathogenic microorganisms.

[0067] In this embodiment, a number of germicidal lamps 45 are fixedly installed on one side of the two vertical partitions 2 close to each other, providing efficient sterilization and disinfection functions for the test room. These germicidal lamps 45 are located in the two test rooms, ensuring that the internal environment of the test room is thoroughly purified before the pathogen resistance test is carried out, eliminating potential pollution sources, and laying a foundation for the smooth progress of the experiment. This design not only enhances the safety of the experimental environment, but also improves the reliability and effectiveness of the experimental results. By performing sterilization and disinfection in advance, the interference of external bacteria is effectively avoided, and the purity and consistency of the experimental conditions are ensured. The introduction of the germicidal lamp 45 not only provides a strong guarantee for the preliminary preparation of the experiment, but also simplifies the experimental process, saves experimental time, and creates favorable conditions for the efficient implementation of pathogen resistance detection.

[0068] In a further preferred embodiment of the present invention, a control panel 101 is provided in the detection box 1 for setting parameters and controlling equipment operation, and two front door panels 102 are provided on the front side of the detection box 1, corresponding to two detection chambers for taking and placing culture dishes 26, and visual glass observation windows are provided on the two front door panels 102, and the rear side of the detection box 1 is set as an opening and is installed with a rear inspection panel 103.

[0069] In this embodiment, the control panel 101 provided in the detection box 1 becomes the "brain" of the entire device, responsible for parameter setting and equipment operation control, ensuring the accuracy and automation of the experimental process. The two front door panels 102 correspond to the two detection chambers, which are convenient for the experimenter to easily take and put the culture dish 26. The visual glass observation window equipped on it allows real-time monitoring of the internal situation during the experiment, which not only ensures the safety of the experiment, but also improves the experimental efficiency. The rear opening and the detachable design of the rear inspection panel 103 ensure the convenience of equipment maintenance and troubleshooting.

[0070] It should be noted that the ratchet mechanism 41 includes a ratchet 411, a driven wheel 412, and a pawl 413. The principle of the ratchet mechanism 41 is the same as that of the ratchet mechanism on the market, which belongs to the existing mature technology, and its structural principle will not be described in detail here. Fig.17 From the perspective shown, when the ratchet 411 rotates counterclockwise, the driven wheel 412 can be driven to rotate through the pawl 413; when the ratchet 411 rotates clockwise, the driven wheel 412 does not rotate. Thus, when the dual-axis motor 35 is started, only one of the rotating shafts 38 rotates, and when the first placement seat 4 and the second placement seat 10 rotate synchronously, the storage plate 25 does not rotate; when the storage plate 25 rotates, the first placement seat 4 and the second placement seat 10 do not rotate.

[0071] The ingenious design of the ratchet mechanism 41 ensures the unidirectional rotation characteristics of the rotating shaft 38, thereby realizing independent and coordinated movements between the first placement seat 4 and the second placement seat 10 and the storage plate 25. When the connecting block 37 rotates counterclockwise under the drive of the dual-axis motor 35, the ratchet 411 drives the driven wheel 412 to rotate through the pawl 413, thereby driving one rotating shaft 38 to rotate while the other remains stationary. Conversely, when rotating clockwise, the other rotating shaft 38 responds, realizing alternating operation of the two rotating shafts, ensuring the independent movement of the first placement seat 4 and the second placement seat 10, while the storage plate 25 rotates independently when needed without affecting each other.

[0072] It is worth noting that the circuits, electronic components and modules involved in the present invention are all prior art, and those skilled in the art can fully implement them. Needless to say, the content protected by the present invention does not involve improvements to software and methods.

[0073] The present solution also provides a controller, which is disposed on the device. When in use, the controller can be used to start each electrical device to automatically operate, and the cooling and heat generation of the semiconductor refrigeration plate 42 can be automatically temperature controlled. The power connection method of each electrical device is an existing mature technology, and the control circuit of the controller can be realized by simple programming by technicians in this field. It is a well-known technology in this field and will not be elaborated here.

[0074] In summary, compared with the related art, the device of the present invention can simultaneously explore the resistance detection of pathogenic microorganisms under different control temperatures and drug concentrations, which is of great significance for the screening of new drugs and the re-evaluation of existing drugs, because it can reveal the changes in the sensitivity of pathogenic microorganisms to drugs, thereby guiding the formulation and optimization of clinical treatment plans.

[0075] In addition, the device of the present invention reduces human operation errors and improves the repeatability and accuracy of detection through an automated detection process.

[0076] In the several embodiments provided in this application, it should be understood that the disclosed device can be implemented in other ways.

[0077] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the scope of protection of the invention. Obviously, the described embodiments are only some embodiments of the present invention, rather than all embodiments. Based on these embodiments, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention. Although the present invention has been described in detail with reference to the above embodiments, ordinary technicians in this field can still combine, add, delete or make other adjustments to the features in the various embodiments of the present invention according to the circumstances without conflict, without making creative work, so as to obtain different other technical solutions that do not deviate from the concept of the present invention in essence, and these technical solutions also belong to the scope of protection of the present invention.

Claims

1. A pathogenic microorganism resistance detection device, characterized in that: include: A detection box (1) for pathogenic microorganism resistance detection, wherein two vertical partitions (2) are fixedly installed in the detection box (1), and a transverse partition (3) is fixedly installed between the two vertical partitions (2), and the transverse partition (3) divides the space between the two vertical partitions (2) into two upper and lower detection chambers, namely a hot detection chamber and a cold detection chamber; Two first placement seats (4) are arranged in the two detection chambers, and the first placement seats (4) are provided with a plurality of placement grooves distributed in an annular manner for placing a plurality of culture dishes (26) for culturing pathogenic microorganisms; The detection box (1) is provided with a drug injection mechanism for injecting a drug for detecting the resistance of pathogenic microorganisms into the culture dish (26); Furthermore, a first transmission mechanism is provided in the detection box (1) for causing the two first placement seats (4) to rotate simultaneously to switch the culture dishes (26).

2. The pathogenic microorganism resistance detection device according to claim 1, characterized in that: Two mounting plates (5) are fixedly mounted between the two vertical partitions (2) for supporting the two first placement seats (4), and the first transmission mechanism comprises: Two first central shafts (6) are respectively fixedly mounted on the bottoms of the two first placement seats (4), and the two first central shafts (6) are respectively rotatably connected to the two mounting plates (5); Rotating a first rotating rod (7) installed on one side of one of the vertical partitions (2); Four first synchronous pulleys (8) are respectively fixedly mounted on the bottom ends of the two first central shafts (6) and the first rotating rod (7), and a first synchronous belt (9) is respectively mounted between the two first synchronous pulleys (8) at the bottom ends of the two first central shafts (6) and the two first synchronous pulleys (8) on the first rotating rod (7); The first transmission mechanism is also equipped with a power drive mechanism for driving the first rotating rod (7) in the first transmission mechanism to rotate, so as to realize the rotation of the first placement seat (4).

3. The pathogenic microorganism resistance detection device according to claim 2, characterized in that: A second placement seat (10) is arranged above the top of the detection box (1), and a plurality of placement slots distributed in an annular shape are provided on the second placement seat (10) for placing a plurality of medicine bottles (11) containing medicines of different concentrations, so as to provide medicines of different concentrations for pathogenic microorganism resistance detection. A second transmission mechanism is installed in the detection box (1) for driving the second placement seat (10) to rotate so as to switch different medicine bottles (11).

4. The pathogenic microorganism resistance detection device according to claim 3, characterized in that: The second transmission mechanism comprises: A second central axis (12) fixedly mounted on the bottom of the second placement seat (10), the second central axis (12) being rotatably connected to the top of the detection box (1); Two second synchronous pulleys (13) are respectively fixedly sleeved on the bottom end of the second central shaft (12) and the first rotating rod (7), and a second synchronous belt (14) is sleeved between the two second synchronous pulleys (13).

5. The pathogenic microorganism resistance detection device according to claim 4, characterized in that: A plurality of placement slots on the first placement seat (4) are each provided with a placement plate (25), a placement slot for placing a culture dish (26) is provided on the top of the placement plate (25), and a third transmission mechanism is provided in the detection box (1), for driving the corresponding placement plates (25) on the two first placement seats (4) to rotate synchronously, so that the medicine can be evenly sprayed into the entire culture dish (26), and the third transmission mechanism comprises: A plurality of third central shafts (27) respectively fixedly mounted on the bottom of the plurality of storage plates (25), the plurality of third central shafts (27) being rotatably connected to the two first storage seats (4) respectively; A plurality of first spur gears (28) respectively fixedly sleeved on the bottom ends of a plurality of third middle shafts (27); Two fourth central shafts (29) are rotatably mounted on the two mounting plates (5), the top ends of the two fourth central shafts (29) are fixedly sleeved with second spur gears (30), and the two second spur gears (30) are respectively meshed with the two first spur gears (28); A second rotating rod (31) is rotatably mounted on a side of the vertical partition (2) away from the first rotating rod (7), and two third synchronous pulleys (32) are fixedly sleeved on the second rotating rod (31); Two fourth synchronous pulleys (33) are respectively fixedly sleeved on the bottom ends of the two fourth central shafts (29), and the two fourth synchronous pulleys (33) and the two third synchronous pulleys (32) are respectively sleeved with third synchronous belts (34); The power drive mechanism is also used to drive the second rotating rod (31) to rotate, so as to rotate the corresponding two storage plates (25).

6. The pathogenic microorganism resistance detection device according to claim 5, characterized in that: The power drive mechanism comprises: A double-shaft motor (35) fixedly mounted on the inner wall of the bottom of the detection box (1); Two connecting shafts (36) respectively fixedly mounted on the two output shafts of the dual-shaft motor (35), and a connecting block (37) is fixedly mounted on ends of the two connecting shafts (36) that are away from each other; Two rotating shafts (38) are rotatably mounted on the two vertical partitions (2), and the bottom end of the first rotating rod (7) and one end of the corresponding rotating shaft (38) are both fixedly sleeved with a first bevel gear (39), and the two first bevel gears (39) are meshed with each other; Two second bevel gears (40) are respectively fixedly sleeved on the second rotating rod (31) and one end of another rotating shaft (38), and the two second bevel gears (40) are meshed with each other; A ratchet mechanism (41) is connected between the rotating shaft (38) and the connecting block (37), and the two ratchet mechanisms (41) are symmetrically arranged to enable the two rotating shafts (38) to rotate only in one direction and in the opposite direction.

7. The pathogenic microorganism resistance detection device according to claim 1, characterized in that: A semiconductor cooling plate (42) is embedded on the transverse partition (3), with the heat generating side of the semiconductor cooling plate (42) facing upward and the cooling side facing downward. Fans (43) are installed on both sides of the semiconductor cooling plate (42), and a plurality of heat dissipation holes (501) are provided on the upper mounting plate (5) for allowing heat to pass through the heat dissipation holes (501) to heat the thermal detection chamber.

8. The pathogenic microorganism resistance detection device according to claim 1, characterized in that: Two temperature sensors (44) are installed on one of the vertical partitions (2), and probes of the two temperature sensors (44) extend into two detection chambers respectively.

9. The pathogenic microorganism resistance detection device according to claim 1, characterized in that: A plurality of sterilizing lamps (45) are fixedly mounted on the sides of the two vertical partitions (2) close to each other. The plurality of sterilizing lamps (45) are located in the two detection chambers and are used to sterilize and disinfect the two detection chambers before carrying out resistance testing on pathogenic microorganisms.

10. The pathogenic microorganism resistance detection device according to claim 1, characterized in that: The detection box (1) is provided with a control panel (101) for setting parameters and controlling the operation of the equipment, and the front side of the detection box (1) is provided with two front door panels (102), corresponding to two detection chambers, for taking in and placing culture dishes (26), and the two front door panels (102) are provided with visual glass observation windows, and the rear side of the detection box (1) is arranged as an opening and is installed with a rear inspection panel (103).

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