Preparation method and application of Luohanshen resistant starch

Abstract

The invention discloses a preparation method and application of Luohanshen resistant starch, and belongs to the technical field of medicine preparation. The preparation method comprises the following steps: adding probiotics (lactobacillus and bifidobacterium) into homogenate of peeled fresh Luohanshen, fermenting, filtering, aging, centrifuging, carrying out alcohol treatment, and drying to obtain the Luohanshen resistant starch. The Luohanshen resistant starch can play a role in preventing and / or treating constipation, remarkably promote the advancing distance of the activated carbon in small intestines of constipation mice, shorten the discharge time of the first black excrement, increase the number of black excrement discharged in 6 hours and the wet weight of the black excrement discharged in 6 hours, increase the content of MTL and SP in serum and increase the content of lactic acid, acetic acid, propionic acid and butyric acid in excrement. According to the preparation method, probiotics (lactobacillus and bifidobacterium) are adopted for synergistic aging to prepare the Luohanshen resistant starch, efficient preparation of the Luohanshen resistant starch is achieved, the content of the Luohanshen resistant starch is 76.89%, the process is simple, the product purity is high, energy consumption is low, and the preparation method is suitable for industrial production.

Description

Technical Field

[0001] The invention belongs to the technical field of medicine preparation methods, and in particular relates to a preparation method and application of radix polygoni ovata resistant starch. Background Art

[0002] The statements herein merely provide background information related to the present invention and do not necessarily constitute prior art.

[0003] Constipation is a common gastrointestinal disease characterized by defecation disorders, such as straining to defecate, prolonged defecation time, difficult defecation, pain and abdominal pain caused by straining to defecate, etc. The prevalence rate among adults worldwide is 2-28%. Constipation can cause great pain and inconvenience to patients, and it may be related to diseases of the respiratory, cardiovascular and nervous systems. Unhealthy lifestyles lead to intestinal diseases and abnormal defecation, but the pathogenesis of constipation is still unclear. At present, drugs for the treatment of constipation are mainly concentrated on laxatives and prokinetic drugs. However, long-term use of these drugs may lead to drug dependence and other adverse reactions, such as dehydration, hypotension, tachycardia, dizziness, abdominal distension and abdominal cramps. Therefore, it is necessary to explore safe and effective alternative treatment options. Nutritional therapy such as dietary adjustment is a good choice for the treatment of constipation. It is very necessary to ingest anti-constipation functional substances from food to improve, prevent and treat constipation. At present, more and more food-borne functional ingredients have been discovered to inhibit constipation.

[0004] Prebiotics can widely play the role of moisturizing the intestines and relieving constipation, and are potential functional factors for improving constipation. The mechanism is that beneficial intestinal bacteria, especially bifidobacteria, can ferment prebiotics to produce a large amount of short-chain fatty acids (SCFAs), which can stimulate intestinal peristalsis and increase the wetness of feces. For example, polysaccharide prebiotics extracted from traditional Chinese medicine, fruits and vegetables, mushrooms, etc. have received widespread attention. These sugars have good anti-constipation effects and fewer side effects, and have potential clinical application value. Exploring more food-borne active ingredients for inhibiting constipation and in-depth research on the mechanism of action are of great significance to human health.

[0005] Luohanshen (commonly known as taro), mainly planted in the plain area of ​​southwest Shandong, is a perennial creeping herb. Its tubers are spherical or irregularly oblong, the skin is earthy yellow, with intermittent ring-shaped textures, and the flesh is white and tender. Luohanshen contains a lot of resistant starch and is a health resource with good development and application prospects. Studies have shown that the content of resistant starch in raw and cooked Luohanshen is higher than that in four common tubers, such as potatoes, taro, sweet potatoes and yam. Therefore, it is an ideal raw material for preparing resistant starch. At present, the known functions of Luohanshen include lowering blood sugar and improving glucose tolerance in diabetic mice. At present, Luohanshen on the domestic market is still mainly used as a medicinal diet, and there is no further processing of Luohanshen as a raw material to prepare active ingredients and use them in the preparation of anti-constipation products. The research on the extraction technology of bioactive ingredients in Luohanshen is the focus of Luohanshen application research. For the preparation of resistant starch, the methods reported in the literature are mainly physical method, enzymatic method and composite method. Traditional methods such as autoclaving, ultrasound, ultra-high pressure, extrusion, microwave puffing, and single enzyme hydrolysis are simple and short in processing time, but the content of resistant starch in the prepared products is poor. The physical method of combining multiple autoclave-cooling treatments can increase the content of resistant starch, but the production cycle is long and the production cost is high. The multi-enzyme combined hydrolysis method can significantly increase the content and purity of resistant starch, but the prepared products have too much residual enzymolyte and enzymolyte products, which mainly contain protein and inorganic salts. Since protein is light yellow in color, a high protein content will affect the whiteness of starch, resulting in low whiteness of the product. Summary of the invention

[0006] In view of the shortcomings of the prior art, the purpose of the present invention is to provide a method for preparing radix serrata resistant starch and its application. The present invention utilizes a fermentation method and an aging method to achieve efficient preparation of radix serrata resistant starch.

[0007] In order to achieve the above object, the present invention provides the following technical solutions:

[0008] In a first aspect, the present invention provides a method for preparing scutellaria baicalensis resistant starch, wherein the scutellaria baicalensis resistant starch is prepared by a fermentation method and a coordinated aging method, comprising the following steps:

[0009] The method comprises adding probiotics to the homogenate of peeled fresh fruits of Radix Panacis radix, fermenting, filtering, aging, centrifuging, treating with alcohol, and drying to obtain the probiotics; the probiotics are lactobacillus and bifidobacterium.

[0010] The inventors unexpectedly discovered during the research process that the combined fermentation of Lactobacillus and Bifidobacterium can significantly increase the content of resistant starch in Lohan ginseng prepared by the method.

[0011] Preferably, the lactobacillus is selected from one or more of Lactobacillus reuteri CICC-6132, Lactobacillus casei NRRL B-1922, Lactobacillus rhamnosus AS1.2466, Lactobacillus rhamnosus HN 001, Lactobacillus acidophilus NRRL B-4495, Lactobacillus brevis NRRL B-4527, Lactobacillus mansoni ATCC 7830, Lactobacillus bulgaricus ATCC 11842, Lactobacillus delbrueckii CICC 6045, Lactobacillus plantarum LP 194, Lactobacillus acidophilus TYCA 06 and Lactobacillus helveticus R0052; the bifidobacterium is selected from one or more of Bifidobacterium longum NRRL B-41409, Bifidobacterium breve NRRL B-41408, Bifidobacterium breve M-16V, Bifidobacterium adolescentis ATCC 15703, Bifidobacterium bifidum ATCC 29521, Bifidobacterium bifidum R0071, Bifidobacterium lactis HN 019. One or more of Bifidobacterium animalis BB-12 and Bifidobacterium infantis R0033.

[0012] In one or more embodiments, the peeled fresh radix serrata fruit is prepared according to the following steps: selecting radix serrata that is free of mold and insects, washing with clean water to remove surface dirt, peeling, draining and naturally drying.

[0013] Furthermore, the fresh radix polygoni ginseng fruit homogenate is obtained by mixing peeled fresh radix polygoni ginseng fruit with water and breaking the wall.

[0014] Furthermore, the peeled fresh fruits of Radix Lohanae are mixed with water and homogenized, and the solid-liquid ratio of the peeled fresh fruits of Radix Lohanae to water is 1:2 to 10, calculated in g / mL; preferably 1:3.

[0015] In one or more embodiments, the pH is adjusted to 3.5-6 before adding the probiotics.

[0016] Furthermore, the total inoculation amount of the lactobacillus and bifidobacterium is 0.2% to 5% of the weight of the peeled fresh fruit of Panax quinquefolius, preferably 1 to 2%.

[0017] Furthermore, the lactobacillus and bifidobacterium work together, and the mass ratio of lactobacillus to bifidobacterium is 1:10 to 10:1, preferably 2:3 to 3:2.

[0018] In one or more embodiments, the fermentation temperature and time are 30-45° C. for 12-80 h, respectively, preferably 35-40° C. for 24-48 h.

[0019] In one or more embodiments, the filtration is performed through 2 to 4 layers of gauze, which is repeated 2 to 4 times.

[0020] In one or more embodiments, the aging temperature and time are 0-10° C. and 24-120 h, respectively, preferably aging at 0-4° C. for 24-48 h.

[0021] In one or more embodiments, after the aging is completed, the supernatant is removed by centrifugation to obtain a precipitate. The centrifugation to remove the precipitate is performed at 8000 to 15000 rpm for 10 to 20 minutes.

[0022] Furthermore, the alcohol is ethanol; the mass ratio of the precipitate to the alcohol is 1:(2-6); preferably 1:(2-3).

[0023] Furthermore, the alcohol treatment is carried out at a temperature of 4 to 25° C. for 6 to 24 hours, preferably at a temperature of 4 to 10° C. for 10 to 14 hours.

[0024] In a second aspect, the present invention provides Lohan radix resistant starch prepared by the above method.

[0025] In a third aspect, the present invention provides the use of the resistant starch prepared by the above method in the preparation of a product for moisturizing the intestine and relieving constipation, wherein the product is a health food or a functional food.

[0026] In a fourth aspect, the present invention provides the use of the resistant starch prepared by the above method in at least one of the following aspects:

[0027] 1) Application in the preparation of products for preventing and / or treating constipation;

[0028] 2) Application in the preparation of products for promoting intestinal motility;

[0029] 3) Application in the preparation of products for improving defecation parameters;

[0030] 4) Application in the preparation of products for increasing serum factor content;

[0031] 5) Application in the preparation of products with increased organic acid content;

[0032] The product described is a pharmaceutical product.

[0033] In one or more embodiments, the constipation is functional constipation or chronic constipation.

[0034] In one or more embodiments, the intestine is the colon, cecum or small intestine, specifically the small intestine tissue of mice.

[0035] In one or more embodiments, the defecation improvement parameter is a constipation improvement or constipation defecation parameter. The defecation parameter may specifically be the time of first black stool discharge, the number of black stool particles discharged in 6 hours, and the wet weight of black stool discharged in 6 hours.

[0036] In one or more embodiments, the serum factor for improving constipation is a serum factor for improving constipation or constipation. The serum factor may specifically be motilin (MTL) and substance P (SP).

[0037] In one or more embodiments, the increasing of organic acid content is increasing the organic acid content in feces. The organic acid is lactic acid, acetic acid, propionic acid, butyric acid, etc.

[0038] When the radix polygoni serrata resistant starch is used as a functional food or a health food, it can be used alone or mixed with other ingredients.

[0039] In clinical application, it is used as a food component in special medical food or health food, which helps to moisten the intestines and relieve constipation, and prevent and / or treat constipation. It can also be used alone or in combination with other drugs to prepare drugs of different dosage forms that can be used clinically by conventional preparation processes, such as adding excipients or flavoring agents and preservatives to prepare capsules for preventing and / or treating constipation or oral liquids for preventing and / or treating constipation.

[0040] In some embodiments, the dosage form of the drug includes capsules, oral solutions, injection solutions, tablets, powders or granules.

[0041] Compared with the prior art, the present invention has the following beneficial effects:

[0042] (1) The present invention adopts probiotics (Lactobacillus and Bifidobacterium) to prepare safflower resistant starch by synergistic aging, thereby achieving efficient preparation of safflower resistant starch. The content of safflower resistant starch is 76.89%. The process is simple, the product purity is high, the energy consumption is low, and it is suitable for industrial production.

[0043] (2) The present invention utilizes probiotics (Lactobacillus and Bifidobacterium) to prepare the Luo Han Shen resistant starch by synergistic aging. In the prevention and / or treatment of constipation, the probiotics and aging play a synergistic role, which can significantly prevent and / or treat constipation, significantly promote the propulsion distance of activated carbon in the small intestine of constipated mice, reduce the discharge time of the first black stool, increase the number of black stool particles discharged in 6 hours and the wet weight of black stool discharged in 6 hours, increase the MTL and SP contents in serum, and increase the contents of lactic acid, acetic acid, propionic acid and butyric acid in feces; it has the effect of moisturizing the intestine and promoting bowel movements.

[0044] (3) The Luo Han Shen resistant starch prepared by the co-aging of probiotics (Lactobacillus and Bifidobacterium) in the present invention significantly promoted the small intestinal peristalsis of mice with constipation induced by diphenoxylate hydrochloride, improved constipation parameters and serum factor content, and increased the content of organic acids in feces. The present invention provides a new option for treating or preventing constipation. BRIEF DESCRIPTION OF THE DRAWINGS

[0045] The accompanying drawings in the specification, which constitute a part of the present invention, are used to provide a further understanding of the present invention. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute improper limitations on the present invention.

[0046] Figure 1 Effects of the scutellaria baicalensis resistant starch prepared in Example 1 and Comparative Examples 1 and 2 of the present invention on the small intestinal propulsion rate of mice with diphenoxylate hydrochloride-induced constipation; wherein (A) is the small intestinal length, (B) is the activated carbon propulsion distance, and (C) is the small intestinal propulsion rate; data are expressed as mean ± SEM, and compared with the control group, *p < 0.05 is significant, and **p < 0.01 is extremely significant; compared with the model group, #p < 0.05 is significant, and ##p < 0.01 is extremely significant;

[0047] Figure 2 Effects of the resistant starch of Radix Lohanii prepared in Example 1 and Comparative Examples 1 and 2 of the present invention on defecation parameters of mice with constipation induced by diphenoxylate hydrochloride; wherein, (A) is the time of discharge of the first black stool, (B) is the number of black stool particles discharged in 6 hours, and (C) is the wet weight of black stool discharged in 6 hours; data are expressed as mean ± SEM, and compared with the control group, *p<0.05 is significant, **p<0.01 is extremely significant; compared with the model group, #p<0.05 is significant, and ##p<0.01 is extremely significant;

[0048] Figure 3 Effects of the scutellaria baicalensis resistant starch prepared in Example 1 and Comparative Examples 1 and 2 of the present invention on the serum factor content of mice with constipation induced by diphenoxylate hydrochloride; wherein, (A) is the MTL content, and (B) is the SP content; the data are expressed as mean ± SEM, and compared with the control group, *p<0.05 is significant, and **p<0.01 is extremely significant; compared with the model group, #p<0.05 is significant, and ##p<0.01 is extremely significant;

[0049] Figure 4 Effects of the spatholobi resistant starch prepared in Example 1 and Comparative Examples 1 and 2 of the present invention on the content of organic acids in feces of mice with diphenoxylate hydrochloride-induced constipation; wherein, (A) is the lactic acid content, (B) is the acetic acid content, (C) is the propionic acid content, and (D) is the butyric acid content; the data are expressed as mean ± SEM, and compared with the control group, *p < 0.05 is significant, and **p < 0.01 is extremely significant; compared with the model group, #p < 0.05 is significant, and ##p < 0.01 is extremely significant. DETAILED DESCRIPTION

[0050] In order to enable those skilled in the art to more clearly understand the technical solution of the present invention, the technical solution of the present invention will be described in detail below in conjunction with specific embodiments.

[0051] Example 1 - Preparation of Radix Glehniae Resistant Starch by Probiotic Fermentation and Aging

[0052] The preparation method of safflower resistant starch is briefly described as follows: after the peeled safflower resistant starch is homogenized, lactobacillus and bifidobacterium are added, fermented, filtered, aged, centrifuged, treated with alcohol, and dried. The specific preparation method is as follows:

[0053] Take 10 kg of peeled fresh Luo Han Shen fruit and add it into a water reaction container, add 30 L of distilled water, and use a wall breaking machine with a power of 500 W to homogenize for 5 minutes.

[0054] Use citric acid to adjust the pH to 3.5-6.0, inoculate 1% (calculated by the weight of peeled fresh fruit of Luo Han Shen) of Lactobacillus rhamnosus AS1.2466 (purchased from Shanghai Xuanke Biotechnology Co., Ltd.) and Bifidobacterium bifidum ATCC 29521 (purchased from Shanghai Fuxiang Biotechnology Co., Ltd.), the mass ratio of Lactobacillus and Bifidobacterium is 3:2, and ferment at 37°C for 48h. After the fermentation is completed, filter the residue with 4 layers of gauze, repeat the filtration 4 times, and place it at 4°C for aging for 48h. After the aging is completed, centrifuge at 10000rpm for 15min to remove the supernatant and take the precipitate, scrape off the upper protein, and repeat centrifugation to remove the protein 3 times. Slowly add 3 times the volume of ethanol to the precipitate (stirring while adding), precipitate at 4°C for 12h, then centrifuge at 5000rpm / min for 10min, remove the supernatant, and take the precipitate.

[0055]

[0056] Starch was prepared by freeze drying (yield: 25.6%), with a whiteness of 87.19 and a purity of 99.1%, of which the content of resistant starch was 76.89%. The composition is shown in Table 1.

[0057] Table 1

[0058]

[0059] Comparative Example 1- Preparation of Radix Glehniae Resistant Starch by Probiotic Fermentation

[0060] The preparation method of safflower resistant starch by fermentation with probiotics is briefly described as follows: after the safflower peeled fruit is homogenized and filtered, probiotics are added for fermentation, filtration, centrifugation, alcohol treatment, and drying are performed. The specific preparation method is as follows:

[0061] Take 10kg of peeled fresh Luo Han Shen fruit and add it to a water reaction container, add 30L of distilled water, and use a wall breaking machine with a power of 500W to homogenize for 5min. Use citric acid to adjust the pH to 3.5-6.0, access 1% (calculated by the weight of peeled Luo Han Shen fruit) of Lactobacillus rhamnosus AS1.2466 and Bifidobacterium bifidum ATCC 29521, the mass ratio of Lactobacillus and Bifidobacterium is 3:2, and ferment at 37°C for 48h. After the fermentation is completed, filter through 2 layers of gauze to remove the filter residue, repeat the filtration twice, centrifuge at 10000rpm for 15min, centrifuge to remove the supernatant and take the precipitate, and scrape off the upper protein. Slowly add 3 times the volume of ethanol to the precipitate (stirring while adding), precipitate at 4°C for 12h, then centrifuge at 5000rpm / min for 10min, remove the supernatant, and take the precipitate. Starch was prepared by freeze drying (yield 18.5%), with a whiteness of 38.54 and a purity of 98.1%, of which the content of resistant starch was 30.56%. Its composition is shown in Table 2.

[0062] Table 2

[0063]

[0064] Comparative Example 2-Preparation of Radix Lohanii Resistant Starch by Aging Method

[0065] The resistant starch of Luo Han Shen was prepared by the aging method. The preparation method is briefly described as follows: the peeled fresh Luo Han Shen fruit is homogenized, aged, centrifuged, treated with alcohol, and dried. The specific preparation method is as follows:

[0066] Take 10kg of peeled fresh fruit of Luo Han Shen and add it to a water reaction container, add 30L of distilled water, and use a wall-breaking machine with a power of 500W to homogenize for 5min. Filter through 2 layers of gauze to remove the filter residue, repeat the filtration twice, and place it at 4°C for aging for 48h. After aging, centrifuge at 10000rpm for 15min to remove the supernatant and take the precipitate, and scrape off the upper protein. Slowly add 3 times the volume of ethanol to the precipitate (stirring while adding), precipitate at 4°C for 12h, then centrifuge at 5000rpm / min for 10min, remove the supernatant, and take the precipitate. Starch is made into starch (yield is 20.6%) after freeze drying, with a whiteness of 40.63 and a purity of 98.3%, of which the resistant starch content is 28.15%. Its composition is shown in Table 3.

[0067] Table 3

[0068]

[0069] Comparative Example 3- Preparation of Radix Polygoni Multiflori Resistant Starch by Lactobacillus Fermentation

[0070] The resistant starch of Luo Han Shen is prepared by fermentation with Lactobacillus. The preparation method is briefly described as follows: after the peeled fresh Luo Han Shen fruit is homogenized and filtered, Lactobacillus is added for fermentation, filtering, centrifugation, alcohol treatment, and drying. The specific preparation method is as follows:

[0071] Take 10kg of peeled fresh Luo Han Shen fruit and add it to a water reaction container, add 30L of distilled water, and use a wall breaking machine with a power of 500W to homogenize for 5min. Use citric acid to adjust the pH to 3.5-6.0, inoculate 1% (calculated by the weight of peeled fresh Luo Han Shen fruit) of Lactobacillus rhamnosus AS1.2466, and ferment at 37°C for 48h. After the fermentation is completed, filter through 2 layers of gauze to remove the filter residue, repeat the filtration twice, centrifuge at 10000rpm for 15min, centrifuge to remove the supernatant and take the precipitate, and scrape off the upper protein. Slowly add 3 times the volume of ethanol to the precipitate (stirring while adding), precipitate at 4°C for 12h, then centrifuge at 5000rpm / min for 10min, remove the supernatant, and take the precipitate. Starch (yield 16.3%) is made by freeze drying, with a whiteness of 26.54 and a purity of 97.9%, of which the resistant starch content is 20.12%, and its composition is shown in Table 4.

[0072] Table 4

[0073]

[0074] Comparative Example 4 - Preparation of Radix Lohanii Resistant Starch by Bifidobacterium Fermentation

[0075] The preparation method of safflower resistant starch is briefly described as follows: after the peeled safflower resistant starch is homogenized and filtered, bifidobacteria are added for fermentation, filtration, centrifugation, alcohol treatment, and drying. The specific preparation method is as follows:

[0076] Take 10kg of peeled fresh fruit of Luo Han Shen and add it to a water reaction container, add 30L of distilled water, and use a wall breaking machine with a power of 500W to homogenize for 5min. Use citric acid to adjust the pH to 3.5-6.0, inoculate 1% (calculated by the weight of peeled fresh fruit of Luo Han Shen) of Bifidobacterium bifidum ATCC 29521, and ferment at 37°C for 48h. After the fermentation is completed, filter through 2 layers of gauze to remove the filter residue, repeat the filtration twice, centrifuge at 10000rpm for 15min, centrifuge to remove the supernatant and take the precipitate, and scrape off the upper protein. Slowly add 3 times the volume of ethanol to the precipitate (stirring while adding), precipitate at 4°C for 12h, then centrifuge at 5000rpm / min for 10min, remove the supernatant, and take the precipitate. Starch (yield of 14.6%) is made by freeze drying, with a whiteness of 24.63 and a purity of 97.6%, of which the resistant starch content is 16.43%. Its composition is shown in Table 5.

[0077] Table 5

[0078]

[0079] Compared with Example 1, the resistant starch content of Comparative Examples 3 and 4 was significantly increased, and other parameters were also improved. It is speculated that the reason is that in this environment, Lactobacillus and Bifidobacterium promote each other's growth and proliferation, produce a large amount of extracellular enzymes and metabolites, and promote the production of resistant starch.

[0080] Application examples:

[0081] 1. Effect of Radix Glehniae Resistant Starch on Small Intestinal Propulsion Rate in Mice with Diphenoxylate Hydrochloride-induced Constipation

[0082] Four-week-old female SPF Kunming mice were fed adaptively for one week in an SPF animal room (temperature: 22±2℃; humidity: 50±10%; lighting: 12h light and dark alternation, 8:00-20:00 as daytime). The mice were divided into 6 groups (8 mice in each group), namely normal group, model group, positive control group, comparative example 1 Luohan resistant starch group, comparative example 2 Luohan resistant starch group and Example 1 Luohan resistant starch group. The gavage administration cycle was 14 days, and mice were gavaged regularly every morning. The normal group and the model group were gavaged with distilled water; the positive control group was gavaged with bisacodyl aqueous solution at a dose of 0.1g / kg; the comparative example 1 Luohan resistant starch group, the comparative example 2 Luohan resistant starch group and the example 1 Luohan resistant starch group were gavaged with the corresponding Luohan resistant starch homogenate at a dose of 1000mg / kg. After 14 days of feeding and gavage of samples, mice were gavaged with corresponding samples on the 15th and 16th days. After gavage of samples, all groups except the normal group were gavaged with 30 mg / kg of diphenoxylate hydrochloride, and the normal group was gavaged with distilled water. On the 17th day, the mice were dissected and the small intestinal propulsion rate, the time of discharge of the first black stool and the wet weight of the black stool discharged in 6 hours, serum SP, MTL and other indicators were measured.

[0083] like Figure 1 As shown in A, compared with the normal group, the length of the small intestine of mice with diphenoxylate hydrochloride-induced constipation was significantly reduced. Compared with the model group, the length of the small intestine of mice in the positive control group, comparative example 1, comparative example 2 and example 1 Luo Han Shen resistant starch intervention group increased by 8.0%, 2.0%, 1.0% and 10.8% respectively (p<0.05). Figure 1 As shown in B, compared with the normal group, the activated carbon propulsion distance of mice with diphenoxylate hydrochloride-induced constipation was significantly reduced. Compared with the model group, the activated carbon propulsion distance of mice in the positive control group and the Luo Han Shen resistant starch intervention group in Example 1 increased significantly, with increases of 122.9% and 115.6%, respectively (both p<0.01), while the increase in comparison example 1 was only 11.2% (p>0.05), and the increase in comparison example 2 was only 5.7% (p>0.05). Figure 1As shown in Figure C, the small intestinal propulsion rate of mice with constipation induced by diphenoxylate hydrochloride was significantly reduced. Compared with the model group, the positive control group and the Luohanshen resistant starch intervention group of mice in Example 1 increased significantly, with increases of 129.7% and 128.9%, respectively (both p<0.01), while the increase in comparative example 1 was only 7.4% (p>0.05), and the increase in comparative example 2 was only 1.9% (p>0.05).

[0084] 2. Effect of Radix Glehniae Resistant Starch on Defecation Parameters of Diphenoxylate Hydrochloride-Induced Constipated Mice

[0085] The animal groups and interventions were the same as above. Figure 2 As shown in A, compared with the normal group, the time of first black stool discharge in mice with diphenoxylate hydrochloride-induced constipation was significantly increased. Compared with the model group, the time of first black stool discharge in the positive control group and the Luo Han Shen resistant starch intervention group of Example 1 was significantly reduced, with a reduction of 40.6% and 36.5%, respectively (both p<0.01), while the reduction in comparative example 1 was only 7.0% (p>0.05), and the reduction in comparative example 2 was only 2.2% (p>0.05). Figure 2 As shown in B-2C, compared with the normal group, the number of black stool particles and the wet weight of black stool discharged at 6 hours in mice with constipation induced by diphenoxylate hydrochloride were significantly reduced. Compared with the model group, the number of black stool particles and the wet weight of black stool discharged at 6 hours in the positive control group and the Luo Han Shen resistant starch intervention group of Example 1 were significantly increased, wherein the increase in Example 1 was 115.4% and 84.6% respectively (both p<0.01), while the increase in Comparative Example 1 was only 19.2% and 7.7% respectively (p>0.05), and the increase in Comparative Example 2 was only 7.8% and 2.3% respectively (p>0.05).

[0086] 3. Effect of Radix Glehniae Resistant Starch on Serum Factor Contents in Mice with Diphenoxylate Hydrochloride-induced Constipation

[0087] The animal groups and interventions were the same as above. Figure 3 As shown in A-3B, compared with the normal group, the serum MTL and SP levels of mice with constipation induced by diphenoxylate hydrochloride were significantly reduced. Compared with the model group, the serum MTL and SP levels of mice in the positive control group and the Luohanshen resistant starch intervention group of Example 1 were significantly increased, wherein the increase in Example 1 was 30.9% and 69.1% respectively (all p<0.01), while the increase in Comparative Example 1 was only 5.6% and 6.9% respectively (p>0.05), and the increase in Comparative Example 2 was only 1.4% and 1.2% respectively (p>0.05).

[0088] 4. Effect of Radix Glehniae Resistant Starch on the Content of Organic Acids in Feces of Mice with Diphenoxylate Hydrochloride-induced Constipation

[0089] Animal grouping and intervention were the same as above. Figure 4 As shown in A-4D, compared with the normal group, the content of lactic acid, acetic acid, propionic acid and butyric acid in the feces of mice with constipation induced by diphenoxylate hydrochloride was significantly reduced. Compared with the model group, the content of lactic acid, acetic acid, propionic acid and butyric acid in the positive control group and the Luohanshen resistant starch intervention group of Example 1 mice increased significantly, among which the Luohanshen resistant starch intervention group of Example 1 mice increased by 42.4%, 59.2%, 105.0% and 292.6% (all p<0.01), while the increase in comparative example 1 was only 4.9%, 9.3%, 13.0% and 19.2% (p>0.05), and the increase in comparative example 2 was only 2.0%, 2.1%, 3.2% and 0.7% (p>0.05).

[0090] In summary, the radix strychnifolia resistant starch prepared by probiotic fermentation and aging in Example 1 has a more significant effect when used, which is much higher than the effects of Comparative Examples 1 and 2, indicating that probiotics and aging have a synergistic effect.

[0091] Analysis of its principle: In the scheme of the present invention, the extracellular enzymes of probiotics during fermentation can hydrolyze the amorphous region and part of the crystalline region of starch granules, reducing the degree of polymerization of starch and shortening the chain length. At the same time, the organic acid produced during the metabolism of probiotics penetrates into the micelles of starch granules, breaking the α-1,4-glycosidic bonds of starch molecules, causing the molecular chain to break into short-length starch chain molecules. The molecular weight of starch becomes smaller, the degree of polymerization decreases, and the ability of starch molecules to recrystallize is enhanced, making it easy to generate resistant starch under aging conditions. Therefore, the use of probiotic fermentation and aging method can significantly increase the content of resistant starch.

[0092] Based on this principle, other lactobacilli and bifidobacteria that secrete extracellular enzymes and produce organic acids can be used for the preparation of radix strychnifoliae resistant starch.

[0093] In some embodiments, the lactobacillus can be one or more of Lactobacillus reuteri CICC-6132, Lactobacillus casei NRRLB-1922, Lactobacillus rhamnosus AS1.2466, Lactobacillus rhamnosus HN 001, Lactobacillus acidophilus NRRL B-4495, Lactobacillus brevis NRRL B-4527, Lactobacillus mansoni ATCC 7830, Lactobacillus bulgaricus ATCC 11842, Lactobacillus delbrueckii CICC6045, Lactobacillus plantarum LP 194, Lactobacillus acidophilus TYCA 06 and Lactobacillus helveticus R0052; the bifidobacterium can be Bifidobacterium longum NRRL B-41409, Bifidobacterium breve NRRL B-41408, Bifidobacterium breve M-16V, Bifidobacterium adolescentis ATCC 15703, Bifidobacterium bifidum ATCC 29521, one or more of Bifidobacterium bifidum R0071, Bifidobacterium lactis HN019, Bifidobacterium animalis BB-12 and Bifidobacterium infantis R0033.

[0094] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A method for preparing radix spatholobi resistant starch, characterized in that: The steps include: The method comprises adding probiotics to the homogenate of peeled fresh fruits of Radix Panacis radix, fermenting, filtering, aging, centrifuging, treating with alcohol, and drying to obtain the probiotics; the probiotics are a combination of lactobacillus and bifidobacterium.

2. The method for preparing radix polygoni rosaefolii resistant starch according to claim 1, characterized in that: The lactobacillus is selected from one or more of Lactobacillus reuteri CICC-6132, Lactobacillus casei NRRL B-1922, Lactobacillus rhamnosus AS 1.2466, Lactobacillus rhamnosus HN 001, Lactobacillus acidophilus NRRL B-4495, Lactobacillus brevis NRRL B-4527, Lactobacillus mansoni ATCC 7830, Lactobacillus bulgaricus ATCC 11842, Lactobacillus delbrueckii CICC 6045, Lactobacillus plantarum LP 194, Lactobacillus acidophilus TYCA06 and Lactobacillus helveticus R0052; the bifidobacterium is selected from one or more of Bifidobacterium longum NRRL B-41409, Bifidobacterium breve NRRL B-41408, Bifidobacterium breve M-16V, Bifidobacterium adolescentis ATCC 15703, Bifidobacterium bifidum ATCC 29521, Bifidobacterium bifidum R0071, Bifidobacterium lactis HN 019. One or more of Bifidobacterium animalis BB-12 and Bifidobacterium infantis R0033.

3. The method for preparing radix polygoni rosaefolii resistant starch according to claim 1, characterized in that: Take the peeled fresh fruit of Radix Lohanae and mix it with water, and process it into a homogenate. The solid-liquid ratio of the peeled fresh fruit of Radix Lohanae to water is 1:2-10, calculated in g / mL; preferably 1:

3.

4. The method for preparing the resistant starch of Radix Polygoni Multiflori according to claim 1, characterized in that: Before adding probiotics, adjust the pH to 3.5-6.

5. The method for preparing radix polygoni rosaefolii resistant starch according to claim 1, characterized in that: The total inoculation amount of the lactobacillus and bifidobacterium is 0.2% to 5% of the weight of the peeled fresh fruit of Radix Panax chinensis, preferably 1% to 2%.

6. The method for preparing the resistant starch of Radix Polygoni Multiflori according to claim 1, characterized in that: The mass ratio of the lactobacillus to the bifidobacterium is 1:10 to 10:1, preferably 2:3 to 3:

2.

7. The method for preparing radix polygoni rosaefolii resistant starch according to claim 1, characterized in that: The fermentation temperature and time are 30-45°C for 12-80 hours, preferably 35-40°C for 24-48 hours.

8. The scutellaria baicalensis resistant starch prepared according to the method for preparing scutellaria baicalensis resistant starch according to any one of claims 1 to 7.

9. Use of the scutellaria baicalensis resistant starch prepared by the method for preparing scutellaria baicalensis resistant starch according to any one of claims 1 to 7 in preparing a product for moisturizing the intestine and facilitating bowel movements, wherein the product is a health food or a functional food.

10. Use of the scutellaria baicalensis resistant starch prepared by the method for preparing scutellaria baicalensis resistant starch according to any one of claims 1 to 7 in at least one of the following aspects: 1) Application in the preparation of products for preventing and / or treating constipation; 2) Application in the preparation of products for promoting intestinal motility; 3) Application in the preparation of products for improving defecation parameters; 4) Application in the preparation of products for increasing serum factor content; 5) Application in the preparation of products for increasing the content of organic acids in feces, wherein the organic acids are lactic acid, acetic acid, propionic acid and butyric acid; The product described is a pharmaceutical product.

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