Novel programmed temperature control device for cryopreserved stem cells and temperature control method of novel programmed temperature control device

Abstract

The invention discloses a novel programmed temperature control device for cryopreservation of stem cells and a temperature control method of the novel programmed temperature control device, and relates to the technical field of cell and bioengineering. The novel programmed temperature control device for cryopreservation of stem cells comprises a refrigerator body, and the front side wall of the refrigerator body is rotationally connected with a cabinet door through a hinge; a display, a controller and a handle are fixedly mounted on the front side wall of the cabinet door, and a cavity is formed in the refrigerator body; the cooling device is arranged in the cavity, the cooling device comprises four supporting rods, and the four supporting rods are fixedly installed in the cavity respectively. The temperature inside the storage box can be finely regulated and controlled, it is ensured that stem cells are stored in a proper low-temperature environment, and the survival rate of the cells and the success rate of subsequent use are increased.

Description

Technical Field

[0001] The present invention relates to the field of cell and bioengineering technology, and specifically to a novel programmable temperature control device and a temperature control method for freezing stem cells. Background Art

[0002] In stem cell research and clinical applications, stem cell cryopreservation is a crucial technology. Stem cells need to be stored at extremely low temperatures to maintain their activity and differentiation potential. However, traditional stem cell cryopreservation devices have many shortcomings in temperature control.

[0003] The traditional program cooling instrument uses an external liquid nitrogen tank for refrigeration. It uses microcomputer control technology and designed software to accurately control the amount of liquid nitrogen released to ensure that the frozen biological products are cooled and frozen at a set appropriate freezing rate, thereby achieving the purpose of program cooling. It is currently widely used in sperm, stem cells, bone marrow, umbilical cord blood, tumors, blood, vaccines, skin tissue, viruses, bacteria and other fields for program cooling, preservation and research. The program cooling instrument has been widely used in the biological industry. Because the instrument uses a liquid nitrogen tank, the liquid nitrogen tank has a short storage time and needs to be frequently added with liquid nitrogen. The use of liquid nitrogen tanks also requires professional knowledge to prevent frostbite caused by contact with the skin during operation. In addition, there are related safety hazards in liquid nitrogen tanks. If the liquid nitrogen tank is under certain conditions, the pressure is too high and there is a risk of explosion. The use of this instrument has problems such as maintenance cost, complex operation, safety hazards, and the temperature drops too quickly during use, which makes stem cells vulnerable to damage during the freezing process, causing ice crystals to form inside the stem cells, thereby destroying the cell structure and affecting its subsequent use effect. Therefore, based on the above search and in combination with the prior art, a new programmable temperature control device and a temperature control method for cryopreservation of stem cells are proposed to solve the above problems. Summary of the invention

[0004] The purpose of the present invention is to provide a novel programmable temperature control device and a temperature control method for cryopreserving stem cells, so as to partially or completely solve the problems raised in the above background technology. To achieve the above purpose, the present invention provides the following technical solutions: In a first aspect, a novel program temperature control device for freezing stem cells comprises a refrigerator body, wherein the front side wall of the refrigerator body is rotatably connected to a cabinet door through a hinge, a display, a controller and a handle are fixedly installed on the front side wall of the cabinet door, and a cavity is opened inside the refrigerator body; a cooling device, wherein the cooling device is arranged in the cavity, and the cooling device comprises: four support rods, wherein the four support rods are respectively fixedly installed inside the cavity, and the four support rods are sequentially installed with a first cold storage plate, a second cold storage plate, a third cold storage plate, a fourth cold storage plate and a fifth cold storage plate from bottom to top, and sliding blocks are fixedly installed on both side walls of the first cold storage plate, the third cold storage plate, the fourth cold storage plate and the fifth cold storage plate, and the cavity Slide grooves are provided on both side walls, the sliding blocks slide in the slide grooves, a plurality of electromagnets are arranged inside the slide grooves, the sliding blocks are made of iron material, the second cold storage plate is fixedly mounted on the outer surfaces of the four support rods, a rectangular groove is provided on the top surface of the second cold storage plate, a mounting groove is provided on one side of the rectangular groove, the rectangular groove is connected with the mounting groove, the cooling device also includes a clamping mechanism and a heat preservation mechanism; a storage box, the storage box is arranged in the rectangular groove, a temperature sensor is fixedly installed inside the mounting groove, the second cold storage plate, the third cold storage plate, the fourth cold storage plate and the fifth cold storage plate are all provided with through openings on the top surfaces, and a driving groove is provided on the top surface of the second cold storage plate located on one side of the rectangular groove.

[0005] Preferably, the clamping mechanism includes: a threaded rod 1, which is rotatably connected to the side wall of the rectangular groove, and is provided with two sections of threads with opposite rotation directions. Both ends of the outer surface of the threaded rod 1 are respectively sleeved with driving blocks, and the driving blocks move in the rectangular groove. The side walls of the two driving blocks are fixedly installed with clamps, and the two clamps fix the two side walls of the storage box. The clamping mechanism also includes a driving assembly.

[0006] Preferably, the drive assembly has a fixed plate, the fixed plate is fixedly mounted on the top surface of the first cold storage plate and passes through the through hole, the side wall of the fixed plate is fixedly mounted with a toothed belt, the interior of the drive groove is rotatably connected to a gear, the gear is meshed with the toothed belt, the interior of the drive groove is located on one side of the gear and is rotatably connected to a rotating column, the outer surface of the rotating column and the side wall of the gear are fixedly mounted with couplings, a belt is arranged between the two couplings, one end of the rotating column extends into the rectangular groove and is connected to the side wall of threaded rod one.

[0007] Preferably, the insulation mechanism includes: two electric push rods, the two electric push rods are respectively arranged on two clamps, the top surfaces of the two clamps are provided with placement grooves, the two electric push rods are respectively fixedly installed in the placement grooves, the output ends of the two electric push rods are fixedly installed with top plates, and the top surface of the second cold storage plate is located on one side of the rectangular groove and has a rotating groove.

[0008] Preferably, the heat preservation mechanism also includes: a rotating rod, which is rotatably connected to the inner wall of the rotating groove, a torsion spring is sleeved on the outer surface of one side of the rotating rod, and a low-temperature insulation cloth is fixedly installed on the outer surface of the rotating rod. The heat preservation mechanism also includes a moving component.

[0009] Preferably, the moving component includes: two moving blocks, the two moving blocks are respectively arranged on the two top plates, and moving grooves are opened on the adjacent surfaces of the two top plates, the inner walls of the two moving grooves are rotatably connected with threaded rods 2, the two moving blocks are respectively sleeved on the outer surfaces of the two threaded rods 2 and move in the moving grooves respectively, and the side walls of the two top plates are fixedly installed with driving motors, and the output shaft of the driving motor is connected to the side walls of threaded rods 2.

[0010] Preferably, two sides of the other end of the low-temperature insulation cloth are respectively connected to the side walls of the two moving blocks.

[0011] Preferably, the side walls of the rectangular groove are provided with limiting grooves, and limiting blocks are fixedly mounted on the side walls of the two driving blocks, and the limiting blocks slide in the limiting grooves.

[0012] In a second aspect, a novel programmable temperature control method for cryopreserving stem cells adopts any one of the novel programmable temperature control devices for cryopreserving stem cells in the first aspect, comprising the following steps: Step S100: Open the cabinet door by the handle, and place the stem cells or blood bags to be frozen into the storage box; the storage box is then placed into the rectangular groove of the second cold storage plate, and the cabinet door is closed; Step S200: After closing the cabinet door, the staff sets the required temperature parameters and control mode through the controller; according to the set temperature parameters, the controller starts the cooling device, and the sliding block moves in the slide groove; the movement of the sliding block drives the first cold storage plate, the third cold storage plate, the fourth cold storage plate and the fifth cold storage plate to approach the second cold storage plate, and the stem cells in the storage box are cooled by cold conduction; in the process of the first cold storage plate approaching the second cold storage plate, the fixed plate and the toothed belt move with the first cold storage plate, driving the gear to rotate; the rotation of the gear is transmitted through the coupling and the belt, so that the threaded rod 1 rotates; the rotation of the threaded rod 1 drives the two driving blocks to approach each other, so that the two splints fix the two side walls of the storage box to prevent it from moving; the temperature sensor continuously monitors the temperature inside the storage box.

[0013] Preferably, step S200 further includes step S300: Step S300: When the temperature drop rate exceeds a predetermined value, the controller starts the electric push rod to lift the third cold storage plate upward to slow down the cooling rate. At the same time, the drive motor starts to rotate the threaded rod 2 connected to the output shaft of the drive motor. The rotation of the threaded rod 2 drives the moving block to move in the moving groove, and then drives the low-temperature insulation cloth to unfold and cover the top of the storage box to achieve the insulation effect. The unfolding and winding of the low-temperature insulation cloth are achieved by the cooperation of the rotating rod and the torsion spring. When the covering needs to be cancelled, the drive motor rotates in the opposite direction, and the rebound force of the torsion spring will cause the low-temperature insulation cloth to automatically wind up.

[0014] Compared with the prior art, the beneficial effects of the present invention are: 1. In the present invention application, by providing a cooling device, compared with the liquid nitrogen program cooling equipment, the ultra-low temperature refrigerator program cooling equipment is adopted, which reduces the daily maintenance of the liquid nitrogen tank, the frequent replacement of liquid nitrogen, the danger caused by the replacement of liquid nitrogen, and the danger of explosion caused by the pressure of the liquid nitrogen tank. The refrigerator-type program cooling equipment is more convenient to use than the liquid nitrogen tank method, and the program senses one-button cooling; 2. In the present application, a heat preservation mechanism is provided so that the temperature sensor monitors the temperature changes of the stem cells or blood bags inside the storage box in real time. When the temperature drops too fast and may threaten the safety of the cell structure, the heat preservation mechanism can respond quickly. Through the coordinated action of the electric push rod and the drive motor, the low-temperature insulation cloth is automatically unfolded to cover the top of the storage box, effectively slowing down the temperature drop rate and avoiding the formation of ice crystals in the cells, thereby protecting the integrity and functional activity of the cell structure. The heat preservation mechanism can also be intelligently adjusted according to the feedback of the temperature sensor. By accurately controlling the unfolding degree and covering time of the low-temperature insulation cloth, the internal temperature of the storage box can be finely regulated to ensure that the stem cells are stored in a suitable low-temperature environment, thereby improving the survival rate of the cells and the success rate of subsequent use. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 The left side structural diagram of the present invention application; Figure 2 This is a schematic diagram of the cabinet door in the open state of the present invention; Figure 3 This is a schematic diagram of the cooling device and the refrigerator body separated from each other in the present invention; Figure 4 A schematic diagram of the top view of the second cold storage plate applied for the present invention; Figure 5 This is a schematic diagram of the right side structure of the second cold storage plate applied for by the present invention; Figure 6 This is a schematic diagram of the overall structure of the clamping mechanism for the present invention; Figure 7 Application for this invention Figure 5 The enlarged structural diagram at A in the middle; Figure 8 Application for this invention Figure 6 Enlarged structural diagram at B in the middle.

[0016] In the figure: 1, refrigerator body; 2, cabinet door; 3, display; 4, controller; 5, handle; 6, cavity; 7, support rod; 8, first cold storage plate; 9, second cold storage plate; 10, third cold storage plate; 11, fourth cold storage plate; 12, fifth cold storage plate; 13, sliding block; 14, slide groove; 15, rectangular groove; 16, mounting groove; 17, storage box; 18, temperature sensor; 19, through port; 20, drive groove; 21, fixed Plate; 22, toothed belt; 23, gear; 24, rotating column; 25, coupling; 26, belt; 27, threaded rod one; 28, driving block; 29, clamping plate; 30, placement slot; 31, electric push rod; 32, top plate; 33, rotating slot; 34, rotating rod; 35, torsion spring; 36, low-temperature insulation cloth; 37, moving slot; 38, threaded rod two; 39, moving block; 40, driving motor; 41, limit slot; 42, limit block. DETAILED DESCRIPTION

[0017] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0018] like Figures 1 to 8 As shown, in the first aspect, a novel programmable temperature control device for freezing stem cells comprises a refrigerator body 1, the front side wall of the refrigerator body 1 is rotatably connected to a cabinet door 2 through a hinge, a display 3, a controller 4 and a handle 5 are fixedly mounted on the front side wall of the cabinet door 2, and a cavity 6 is opened inside the refrigerator body 1; A cooling device is arranged in the cavity 6, and the cooling device comprises: four support rods 7, the four support rods 7 are respectively fixedly mounted inside the cavity 6, the four support rods 7 are sequentially mounted with a first cold storage plate 8, a second cold storage plate 9, a third cold storage plate 10, a fourth cold storage plate 11 and a fifth cold storage plate 12 from bottom to top, both side walls of the first cold storage plate 8, the third cold storage plate 10, the fourth cold storage plate 11 and the fifth cold storage plate 12 are fixedly mounted with sliding blocks 13, both side walls of the cavity 6 are provided with slide grooves 14, the sliding blocks 13 slide in the slide grooves 14, the sliding blocks 13 are made of iron material, the second cold storage plate 9 is fixedly mounted on the outer surface of the four support rods 7, a rectangular groove 15 is provided on the top surface of the second cold storage plate 9, a mounting groove 16 is provided on one side of the rectangular groove 15, and the rectangular groove 15 is connected with the mounting groove 16, and the cooling device also comprises a clamping mechanism and a heat preservation mechanism; A storage box 17 is provided in the rectangular groove 15. A temperature sensor 18 is fixedly installed inside the mounting groove 16. Through openings 19 are provided on the top surfaces of the second cold storage plate 9, the third cold storage plate 10, the fourth cold storage plate 11 and the fifth cold storage plate 12. A driving groove 20 is provided on the top surface of the second cold storage plate 9 located on one side of the rectangular groove 15.

[0019] In some embodiments, a plurality of electromagnets are provided inside the slide groove 14, and the novel program temperature control device for freezing stem cells can move the first cold storage plate 8 upward, and move the third cold storage plate 10, the fourth cold storage plate 11 and the fifth cold storage plate 12 downward to cool the blood bag inside the storage box 17 in the second cold storage plate 9. Specifically, the staff opens the cabinet door 2 through the handle 5, puts the blood bag or other stem cells into the storage box 17, puts the storage box 17 into the rectangular groove 15, and then closes the cabinet door 2. The staff controls the first cold storage plate 8, the third cold storage plate 10, the fourth cold storage plate 11 and the fifth cold storage plate 12 through the controller 4. The third cold storage plate 10, the fourth cold storage plate 11 and the fifth cold storage plate 12 are controlled, and the multiple electromagnets 14 can be independently connected to the driving device. The driving device can start the electromagnets in the slide slot 14 in sequence, driving the sliding block 13 to move in the slide slot 14. When the sliding block 13 moves, the first cold storage plate 8, the third cold storage plate 10, the fourth cold storage plate 11 and the fifth cold storage plate 12 are all close to the second cold storage plate 9 in sequence, and the cold energy of the first cold storage plate 8, the third cold storage plate 10, the fourth cold storage plate 11 and the fifth cold storage plate 12 is transferred to the blood bag to cool it down.

[0020] It is worth mentioning that the cooling device can also cool down in two other ways. First, the blood bag is placed in the storage box 17, and the controller 4 controls the second cold storage plate 9 to make it close to the third cold storage plate 10 to implement temperature control; second, antifreeze can be used to reduce the temperature of the antifreeze to minus 80°C, where the antifreeze becomes a cold storage module. If the temperature is not lowered enough, the antifreeze is circulated to the bottom of the second cold storage plate 9 to transfer the cold to the blood bag.

[0021] In some embodiments, a driving device is provided inside the slide 14, and the driving device drives the sliding block 13 to move up and down. For example, the lifting method of the first cold storage plate 8, the third cold storage plate 10, the fourth cold storage plate 11 and the fifth cold storage plate 12 can also be carried out by mechanical transmission methods, such as motor drive, and hydraulic transmission methods, such as hydraulic cylinder drive, etc. Correspondingly, mechanical transmission or hydraulic cylinder drive can also drive the sliding block to move up and down in the slide.

[0022] As a preferred implementation in this embodiment, please refer to Figures 4 to 6As shown, the clamping mechanism includes: a threaded rod 27, the threaded rod 27 is rotatably connected to the side wall of the rectangular groove 15, the threaded rod 27 is provided with two sections of threads with opposite rotation directions, the two ends of the outer surface of the threaded rod 27 are respectively sleeved with driving blocks 28, the driving blocks 28 move in the rectangular groove 15, the side walls of the two driving blocks 28 are fixedly installed with clamping plates 29, the two clamping plates 29 fix the two side walls of the storage box 17, the clamping mechanism also includes a driving assembly, the driving assembly includes a fixing plate 21, the fixing plate 21 is fixedly installed A toothed belt 22 is fixedly installed on the top surface of the first cold storage plate 8 and passes through the through opening 19. A gear 23 is rotatably connected to the inside of the driving groove 20. The gear 23 meshes with the toothed belt 22. A rotating column 24 is rotatably connected to one side of the gear 23 inside the driving groove 20. A coupling 25 is fixedly installed on the outer surface of the rotating column 24 and the side wall of the gear 23. A belt 26 is arranged between the two couplings 25. One end of the rotating column 24 extends into the rectangular groove 15 and is connected to the side wall of the threaded rod 27.

[0023] In the application of the present invention, the storage box 17 can be fixed to prevent it from moving. Specifically, in the process of the first cold storage plate 8 approaching the second cold storage plate 9, the fixed plate 21 and the toothed belt 22 will be driven to move in the through hole 19, so that the gear 23 rotates. When the gear 23 rotates, the coupling 25 connected to the side wall of the gear 23 will rotate synchronously. When the coupling 25 on the gear 23 rotates, the coupling 25 on the rotating column 24 will rotate synchronously through the transmission of the belt 26, so that the threaded rod 27 rotates. When the threaded rod 27 rotates, the two driving blocks 28 will approach each other. When the driving blocks 28 move, the splints 29 connected to the side walls of the two driving blocks 28 will fix the two sides of the storage box 17. To cancel the fixation, you only need to lower the first cold storage plate 8 to keep it away from the second cold storage plate 9. As a preferred implementation in this embodiment, please refer to Figure 4 , Figure 5 and Figure 8 As shown, the insulation mechanism includes: two electric push rods 31, the two electric push rods 31 are respectively arranged on the two clamping plates 29, the top surfaces of the two clamping plates 29 are provided with placement grooves 30, the two electric push rods 31 are respectively fixedly installed in the placement grooves 30, the output ends of the two electric push rods 31 are fixedly installed with top plates 32, the top surface of the second cold storage plate 9 is located on one side of the rectangular groove 15 and is provided with a rotating groove 33, the insulation mechanism also includes: a rotating rod 34, the rotating rod 34 is rotatably connected to the inner wall of the rotating groove 33, a torsion spring 35 is sleeved on the outer surface of one side of the rotating rod 34, and a low-temperature insulation cloth 36 is fixedly installed on the outer surface of the rotating rod 34, and the insulation mechanism also includes a moving component.

[0024] As a preferred implementation in this embodiment, please refer to Figure 4 , Figure 5and Figure 8 The moving component includes: two moving blocks 39, the two moving blocks 39 are respectively arranged on the two top plates 32, and the adjacent surfaces of the two top plates 32 are provided with moving grooves 37. The inner walls of the two moving grooves 37 are rotatably connected with threaded rods 38. The two moving blocks 39 are respectively sleeved on the outer surfaces of the two threaded rods 38 and move in the moving grooves 37 respectively. The side walls of the two top plates 32 are fixedly installed with driving motors 40, and the output shaft of the driving motor 40 is connected to the side walls of the threaded rods 38. The two sides of the other end of the low-temperature insulation cloth 36 are respectively connected to the side walls of the two moving blocks 39.

[0025] In the application of the present invention, the storage box 17 can be kept warm according to the falling temperature of the storage box 17. When the temperature drops too quickly, ice crystals are formed in the blood bag or other stem cells, causing damage to the cell structure, the temperature sensor 18 detects the temperature of the blood bag or other stem cells inside the storage box 17. When the temperature drops more quickly than a predetermined value, the electric push rod 31 automatically starts to push the third cold storage plate 10 upward. At this time, the drive motor 40 is started to rotate the threaded rod 2 38 connected to the output shaft of the drive motor 40. When the threaded rod 2 38 rotates, the moving block 39 moves in the moving groove 37. When the moving block 39 moves, the low-temperature insulation cloth 36 follows the moving block 39 to cover the top of the storage box 17 to avoid the temperature dropping too quickly. When it is necessary to cancel the covering of the storage box 17, the drive motor 40 only needs to rotate in the opposite direction. At this time, the rotating rod 34 will reel in the low-temperature insulation cloth 36 due to the rebound force of the torsion spring 35.

[0026] It is worth mentioning that the temperature sensor 18 is a thermocouple temperature sensor or a thermal resistor temperature sensor.

[0027] As a preferred implementation in this embodiment, please refer to Figure 5 and Figure 7 As shown, the side wall of the rectangular groove 15 is provided with a limit groove 41, and the side walls of the two driving blocks 28 are fixedly installed with limit blocks 42, and the limit blocks 42 slide in the limit groove 41. In the application of the present invention, the two driving blocks 28 can be limited so that they can slide stably in the rectangular groove 15.

[0028] In a second aspect, a novel programmable temperature control method for cryopreserving stem cells adopts any one of the novel programmable temperature control devices for cryopreserving stem cells in the first aspect, characterized in that it comprises the following steps: Step S100: Open the cabinet door 2 through the handle 5, and place the stem cells or blood bags to be frozen into the storage box 17; the storage box 17 is then placed into the rectangular groove 15 of the second cold storage plate 9, and the cabinet door 2 is closed; Step S200: After closing the cabinet door 2, the staff sets the required temperature parameters and control mode through the controller 4; according to the set temperature parameters, the controller 4 starts the cooling device, and the sliding block 13 moves in the slide groove 14; the movement of the sliding block 13 drives the first cold storage plate 8, the third cold storage plate 10, the fourth cold storage plate 11 and the fifth cold storage plate 12 to approach the second cold storage plate 9, and the stem cells in the storage box 17 are cooled by cold conduction; in the process of the first cold storage plate 8 approaching the second cold storage plate 9, the fixed plate 21 and the toothed belt 22 move with the first cold storage plate 8, driving the gear 23 to rotate; the rotation of the gear 23 is transmitted through the coupling 25 and the belt 26, so that the threaded rod 27 rotates; the rotation of the threaded rod 27 drives the two driving blocks 28 to approach each other, so that the two clamping plates 29 fix the two side walls of the storage box 17 to prevent it from moving; the temperature sensor 18 continuously monitors the temperature inside the storage box 17.

[0029] Step S300: When the temperature drop rate exceeds a predetermined value, the controller 4 starts the electric push rod 31 to lift the third cold storage plate 10 or other appropriate cold storage plates upward to slow down the cooling rate. At the same time, the drive motor 40 starts to rotate the threaded rod 38 connected to the output shaft of the drive motor 40; the rotation of the threaded rod 38 drives the moving block 39 to move in the moving groove 37, thereby driving the low-temperature insulation cloth 36 to unfold and cover the top of the storage box 17 to achieve the insulation effect. The unfolding and winding of the low-temperature insulation cloth 36 are achieved by the cooperation of the rotating rod 34 and the torsion spring 35. When the covering needs to be cancelled, the drive motor 40 rotates in the opposite direction, and the rebound force of the torsion spring 35 causes the low-temperature insulation cloth 36 to automatically wind up.

[0030] The present invention is applied for a new type of programmed temperature control method for freezing stem cells. By providing a heat preservation mechanism, a temperature sensor is enabled to monitor the temperature changes of stem cells or blood bags inside the storage box in real time. When the temperature drops too quickly and may threaten the safety of the cell structure, the heat preservation mechanism can respond quickly. Through the coordinated action of the electric push rod and the drive motor, the low-temperature insulation cloth is automatically unfolded to cover the top of the storage box, effectively slowing down the temperature drop rate and avoiding the formation of ice crystals in the cells, thereby protecting the integrity and functional activity of the cell structure. The heat preservation mechanism can also perform intelligent adjustment according to the feedback of the temperature sensor. By accurately controlling the unfolding degree and covering time of the low-temperature insulation cloth, the temperature inside the storage box can be finely controlled to ensure that the stem cells are stored in a suitable low-temperature environment, thereby improving the survival rate of the cells and the success rate of subsequent use.

[0031] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed in the present invention, and they should be covered by the protection scope of the present invention.

Claims

1. A novel programmable temperature control device for cryopreservation of stem cells, characterized in that: include: A refrigerator body (1), wherein the front side wall of the refrigerator body (1) is rotatably connected to a cabinet door (2) via a hinge, a display (3), a controller (4) and a handle (5) are fixedly mounted on the front side wall of the cabinet door (2), and a cavity (6) is provided inside the refrigerator body (1); and a cooling device, wherein the cooling device is arranged in the cavity (6), and comprises: Four support rods (7), the four support rods (7) are respectively fixedly mounted inside the cavity (6), the four support rods (7) are sequentially mounted with a first cold storage plate (8), a second cold storage plate (9), a third cold storage plate (10), a fourth cold storage plate (11) and a fifth cold storage plate (12) from bottom to top, both side walls of the first cold storage plate (8), the third cold storage plate (10), the fourth cold storage plate (11) and the fifth cold storage plate (12) are fixedly mounted with sliding blocks (13), and both side walls of the cavity (6) are provided with A slide groove (14), wherein the sliding block (13) slides in the slide groove (14), wherein a plurality of electromagnets are arranged inside the slide groove (14), wherein the sliding block (13) is made of iron material, wherein the second cold storage plate (9) is fixedly mounted on the outer surfaces of the four support rods (7), wherein a rectangular groove (15) is provided on the top surface of the second cold storage plate (9), wherein a mounting groove (16) is provided on one side of the rectangular groove (15), wherein the rectangular groove (15) is connected to the mounting groove (16), and the cooling device further comprises a clamping mechanism and a heat preservation mechanism; A storage box (17), the storage box (17) being arranged in the rectangular groove (15), a temperature sensor (18) being fixedly installed inside the installation groove (16), the top surfaces of the second cold storage plate (9), the third cold storage plate (10), the fourth cold storage plate (11) and the fifth cold storage plate (12) being provided with openings (19), and the top surface of the second cold storage plate (9) being located on one side of the rectangular groove (15) being provided with a driving groove (20).

2. A novel programmable temperature control device for cryopreservation of stem cells according to claim 1, characterized in that: The clamping mechanism includes: A threaded rod (27) is rotatably connected to the side wall of the rectangular groove (15), and the threaded rod (27) is provided with two sections of threads with opposite rotation directions. The two ends of the outer surface of the threaded rod (27) are respectively sleeved with driving blocks (28), and the driving blocks (28) move in the rectangular groove (15). The side walls of the two driving blocks (28) are fixedly mounted with clamping plates (29), and the two clamping plates (29) fix the two side walls of the storage box (17). The clamping mechanism also includes a driving component.

3. A novel programmable temperature control device for cryopreservation of stem cells according to claim 2, characterized in that: Drive components A fixed plate (21), the fixed plate (21) is fixedly mounted on the top surface of the first cold storage plate (8) and passes through the through opening (19), a toothed belt (22) is fixedly mounted on the side wall of the fixed plate (21), a gear (23) is rotatably connected to the interior of the driving groove (20), the gear (23) is meshed with the toothed belt (22), a rotating column (24) is rotatably connected to the interior of the driving groove (20) and is located on one side of the gear (23), a coupling (25) is fixedly mounted on the outer surface of the rotating column (24) and the side wall of the gear (23), a belt (26) is arranged between the two couplings (25), and one end of the rotating column (24) extends into the rectangular groove (15) and is connected to the side wall of the threaded rod (27).

4. A novel programmable temperature control device for cryopreservation of stem cells according to claim 3, characterized in that: The insulation mechanism includes: Two electric push rods (31), the two electric push rods (31) are respectively arranged on two clamping plates (29), the top surfaces of the two clamping plates (29) are provided with placement grooves (30), the two electric push rods (31) are respectively fixedly installed in the placement grooves (30), the output ends of the two electric push rods (31) are fixedly installed with top plates (32), and the top surface of the second cold storage plate (9) is located on one side of the rectangular groove (15) and is provided with a rotation groove (33).

5. A novel programmable temperature control device for cryopreservation of stem cells according to claim 4, characterized in that: The heat preservation mechanism also includes: A rotating rod (34) is rotatably connected to the inner wall of the rotating groove (33), a torsion spring (35) is sleeved on the outer surface of one side of the rotating rod (34), a low-temperature insulation cloth (36) is fixedly mounted on the outer surface of the rotating rod (34), and the insulation mechanism further comprises a moving component.

6. A novel programmable temperature control device for cryopreservation of stem cells according to claim 5, characterized in that: The mobile components include: Two moving blocks (39), the two moving blocks (39) are respectively arranged on the two top plates (32), the adjacent surfaces of the two top plates (32) are each provided with a moving groove (37), the inner walls of the two moving grooves (37) are rotatably connected with a second threaded rod (38), the two moving blocks (39) are respectively sleeved on the outer surfaces of the second threaded rod (38) and respectively move in the moving grooves (37), the side walls of the two top plates (32) are each fixedly mounted with a driving motor (40), the output shaft of the driving motor (40) being connected to the side wall of the second threaded rod (38).

7. A novel programmable temperature control device for cryopreservation of stem cells according to claim 6, characterized in that: Both sides of the other end of the low-temperature insulation cloth (36) are respectively connected to the side walls of the two moving blocks (39).

8. A novel programmable temperature control device for cryopreservation of stem cells according to claim 7, characterized in that: A limiting groove (41) is provided on the side wall of the rectangular groove (15), and limiting blocks (42) are fixedly mounted on the side walls of the two driving blocks (28), and the limiting blocks (42) slide in the limiting groove (41).

9. A novel programmable temperature control method for cryopreservation of stem cells, using the novel programmable temperature control device for cryopreservation of stem cells as claimed in claim 8, characterized in that: The following steps are involved: Step S100: Open the cabinet door by the handle, and place the stem cells or blood bags to be frozen into the storage box; the storage box is then placed into the rectangular groove of the second cold storage plate, and the cabinet door is closed; Step S200: After closing the cabinet door, the staff sets the required temperature parameters and control mode through the controller; according to the set temperature parameters, the controller starts the cooling device, and the sliding block moves in the slide groove; the movement of the sliding block drives the first cold storage plate, the third cold storage plate, the fourth cold storage plate and the fifth cold storage plate to approach the second cold storage plate, and the stem cells in the storage box are cooled by cold conduction; in the process of the first cold storage plate approaching the second cold storage plate, the fixed plate and the toothed belt move with the first cold storage plate, driving the gear to rotate; the rotation of the gear is transmitted through the coupling and the belt, so that the threaded rod 1 rotates; the rotation of the threaded rod 1 drives the two driving blocks to approach each other, so that the two splints fix the two side walls of the storage box to prevent it from moving; the temperature sensor continuously monitors the temperature inside the storage box.

10. A novel programmed temperature control method for cryopreservation of stem cells according to claim 9, characterized in that: Step S200 also includes step S300: Step 3S00: When the temperature drop rate exceeds the preset value, the controller starts the electric push rod to lift the third cold storage plate upward to slow down the cooling rate. At the same time, the drive motor starts to rotate the threaded rod 2 connected to the output shaft of the drive motor; the rotation of the threaded rod 2 drives the moving block to move in the moving groove, and then drives the low-temperature insulation cloth to unfold and cover the top of the storage box to achieve the insulation effect. The unfolding and winding of the low-temperature insulation cloth are realized by the cooperation of the rotating rod and the torsion spring. When the covering needs to be cancelled, the drive motor rotates in the opposite direction, and the rebound force of the torsion spring will cause the low-temperature insulation cloth to automatically wind up.

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