A mouse DIO movement model and its construction method

Abstract

The present application relates to the technical field of animal experiments, and specifically discloses a mouse DIO exercise model and a method for constructing it. The method for constructing a mouse DIO exercise model disclosed in the present application specifically includes the following steps: giving experimental mice a high-calorie feed to induce the establishment of a mouse DIO model; grouping and exercising the mice; the exercise intervention includes an exercise pattern combining a running exercise intervention group and a swimming exercise intervention group; performing mouse index detection; and data analysis to obtain a mouse DIO exercise model. The method for constructing a mouse DIO exercise model provided in the present application is simple to operate and feasible, and can effectively reduce the weight of the mouse DIO model, reduce body fat content, and improve blood sugar and blood lipid levels, thereby obtaining a stable mouse DIO exercise model, providing an effective experimental model for studying the pathogenesis and treatment methods of obesity and related diseases.

Description

Technical Field

[0001] The present application relates to the technical field of animal experiments, and in particular to a mouse DIO movement model and a construction method thereof. Background Art

[0002] With the improvement of people's living standards and changes in eating habits, obesity is becoming more and more serious. Obesity not only affects personal image, but also increases the risk of various diseases, such as diabetes and cardiovascular disease.

[0003] In medical research, the mouse DIO model is widely used to study the pathogenesis and treatment of obesity and related diseases. However, there are still some shortcomings in the current exercise intervention method based on the mouse DIO model. The exercise program lacks standardization, the intensity, frequency and duration are not uniform, which affects the comparability of experimental results, there are large individual differences, and the reactions and adaptability of individuals of different strains and the same strain are different, which makes it difficult to control the impact on the results. The mechanism of action is not studied in depth, the interaction of multiple factors is complex and lacks long-term follow-up research, the experimental conditions and technology are limited, the exercise equipment cannot fully simulate natural movement, the detection indicators are limited, and there are differences between the mouse model and humans, which affects the clinical application transformation. These shortcomings limit the effectiveness and promotion of this method. Summary of the invention

[0004] In order to solve the above technical problems, the present application provides a mouse DIO movement model and a construction method thereof.

[0005] The present application provides a method for constructing a mouse DIO movement model, which specifically includes the following steps:

[0006] S1: Giving experimental mice a high-calorie diet to induce a mouse DIO model; the high-calorie diet formula specifically comprises the following components in parts by weight: 96-98 parts of high-fat diet, 2-4 parts of honeysuckle vine extract, 0.4-0.8 parts of pomegranate peel polyphenols, and 0.1-0.3 parts of phaseolin;

[0007] S2: performing grouping and exercise intervention on mice; the exercise intervention includes an exercise mode combining a running exercise intervention group and a swimming exercise intervention group;

[0008] The specific method of the running exercise intervention group program is: using a professional mouse treadmill, running training is performed twice a day, each time for 26-34 minutes, the speed is gradually increased from the initial 3-7m / min to 13-17m / min, and the slope is set during the running process to increase the exercise intensity;

[0009] The specific method of the swimming exercise intervention group program is: swimming training in a constant temperature swimming tank, 2-4 times a week, each time for 15-25 minutes, and the water temperature is maintained at 30-32°C;

[0010] S3: perform mouse index detection;

[0011] S4: Data analysis, obtaining the mouse DIO movement model.

[0012] Preferably, the specific steps of S1 are:

[0013] S1-1: Select C57BL / 6 mice of similar size and weight, 6 to 8 weeks old, as experimental mice;

[0014] S1-2: The experimental mice were placed in a standard feeding environment for one week and given a normal diet;

[0015] S1-3: After the adaptation period, high-calorie diet was given to induce the DIO model, and sufficient drinking water was provided;

[0016] S1-4: Continue to give high-calorie diet for 7-12 weeks, measure the weight and body fat content of mice every week, and when the weight of mice increases by 25% compared with the initial weight and the body fat content increases to 40%, it is determined that the mouse DIO model is successfully established.

[0017] Preferably, the high-calorie feed formula specifically comprises the following components in parts by weight: 96-98 parts of high-fat feed, 2-4 parts of honeysuckle vine extract, 0.4-0.8 parts of pomegranate peel polyphenols, and 0.1-0.3 parts of phaseolin.

[0018] Preferably, the high-calorie feed formula specifically comprises the following components in parts by weight: 97 parts of high-fat feed, 3 parts of honeysuckle vine extract, 0.6 parts of pomegranate peel polyphenols, and 0.2 parts of phaseolin.

[0019] The high-calorie feed provided in this application helps to reduce the occurrence of obesity caused by excessive energy accumulation in experimental mice, increases the obesity index of mice, shortens the period of building a mouse DIO model, and thus helps to reduce the cost of the experiment. In addition, the feed is experimentally safe and can be used for modeling without any worries.

[0020] Preferably, the specific method of placing the experimental mice in a feeding environment for adaptation in S1-2 is:

[0021] Provide a stable breeding environment. The temperature, humidity, and lighting conditions must meet the survival needs of mice. Keep the breeding environment clean and sanitary. Change the bedding regularly to prevent the spread of disease. Avoid environmental noise and interference to reduce the stress response of mice.

[0022] Preferably, the specific method for grouping mice and performing exercise intervention in S3 is:

[0023] S2-1: The successfully established DIO mice were randomly divided into an exercise intervention group and a control group;

[0024] S2-2: The mice in the exercise intervention group underwent an exercise program, including a combined exercise pattern of the running exercise intervention group and the swimming exercise intervention group;

[0025] S2-3: The mice in the control group were maintained in a normal breeding environment without any exercise intervention, and their diet was the same as that of the exercise intervention group.

[0026] Preferably, the specific method of the running exercise intervention group is: using a professional mouse treadmill, performing running training twice a day, each time for 28-32 minutes, the speed gradually increases from the initial 4-6m / min to 14-16m / min, and setting a slope during running to increase the intensity of exercise;

[0027] The specific method of the swimming exercise intervention group program is: swimming training is performed in a constant temperature swimming tank 2-4 times a week, each time for 18-22 minutes, and the water temperature is maintained at 30-32°C to avoid hypothermia of the mice.

[0028] Preferably, the specific method of the running exercise intervention group is: using a professional mouse treadmill, performing running training twice a day, each time for 28-32 minutes, and gradually increasing the speed from the initial 5m / min to 15m / min, and setting an appropriate slope during running to increase the intensity of exercise;

[0029] The specific method of the swimming exercise intervention group program is: swimming training is performed in a constant temperature swimming tank 3 times a week, each time for 20 minutes, and the water temperature is maintained at 30-32°C to avoid hypothermia of the mice.

[0030] Preferably, the specific method for detecting mouse indicators is as follows:

[0031] S3-1: During the exercise intervention, the body weight, body fat content, blood sugar, and blood lipid indexes of mice were regularly tested. The testing time points were set at 2, 4, 6, 8, 10, 12, 14, and 16 weeks after the start of the exercise intervention.

[0032] S3-2: Body weight measurement: Use an electronic scale to accurately measure the body weight of mice. Each measurement should be performed at the same time to reduce errors.

[0033] S3-3: Body fat content detection: Dual-energy X-ray absorptiometry or magnetic resonance imaging was used to measure the body fat content of mice and evaluate the fat distribution;

[0034] S3-4: Blood glucose and blood lipid testing: The blood of mice was collected by tail tip blood sampling method, and blood glucose and blood lipid levels were tested using a blood glucose meter and a blood lipid tester, respectively, including fasting blood glucose, postprandial blood glucose, total cholesterol, triglycerides, high-density lipoprotein cholesterol and low-density lipoprotein cholesterol.

[0035] Preferably, the specific method in the data analysis step is as follows:

[0036] S4-1: Statistical analysis was performed on various indicators of mice in the exercise intervention group and the control group, and the differences between the two groups were compared. The statistical methods of t-test or variance analysis were used to determine the effect of exercise intervention;

[0037] S4-2: Analyze the effects of exercise intervention on the DIO exercise model in mice, including weight loss, body fat reduction, and lower blood sugar and blood lipid levels, and visually demonstrate the changing trend of the effect of exercise intervention over time;

[0038] S4-3: Based on the literature and existing research results, further explore the mechanism of action of exercise intervention.

[0039] In some specific embodiments, the modeling period of the mouse DIO exercise model is 12-16 weeks; when various detection indicators of the experimental mice reach a plateau, the mouse DIO exercise model is established.

[0040] In summary, the technical solution of this application has the following effects:

[0041] The present application provides an exercise intervention method based on the mouse DIO model to obtain a mouse DIO exercise model, which is simple and feasible to operate, and can effectively reduce the weight of mice, reduce body fat content, and improve blood sugar and blood lipid levels, thereby obtaining a stable mouse DIO exercise model.

[0042] The method and mouse DIO exercise model provided in this application provide an effective experimental means and experimental model for studying the pathogenesis and treatment methods of obesity and its related diseases. By analyzing the effects of exercise intervention, we can gain an in-depth understanding of the therapeutic mechanism of exercise on obesity and provide a theoretical basis for the development of new obesity treatment methods. DETAILED DESCRIPTION

[0043] The present application is further described in detail below in conjunction with examples, comparative examples and performance testing experiments. These examples should not be construed as limiting the scope of protection claimed in the present application.

[0044] The high-fat feed used in this application is Sino D12492; honeysuckle vine extract (product number RDTTQW01) and white kidney bean extract (product number BYDTQW01) were purchased from Xi'an Shizeyuan Biotechnology Co., Ltd.; pomegranate peel polyphenols (product number MF-009298), phaseol (product number MF-013225), tea polyphenols (product number MF-008921), and quercetin (product number MF-003025) were purchased from Xinyang Mufan Biotechnology Co., Ltd.; the remaining raw materials used can be obtained through commercial purchase.

[0045] Unless the context clearly dictates otherwise, unmodified nouns and nouns modified by "the" include singular and plural referents.

[0046] As used in the specification and claims, the terms "comprises," "comprising," "having," "may," "containing," and variations thereof as used herein refer to open transitional phrases, terms, or words that require the presence of specified ingredients / steps and allow for the presence of other ingredients / steps. However, such descriptions should be interpreted as also describing compositions or methods as "consisting of" and "consisting essentially of" the recited ingredients / steps, which allows for the presence of only the specified ingredients / steps and any unavoidable impurities that may result therefrom, and excludes other ingredients / steps.

[0047] Numerical values ​​in the specification and claims of this application should be understood to include the same numerical values ​​when reduced to the same number of significant figures and numerical values ​​that differ from the stated value by less than the experimental error of ordinary measurement techniques of the type described in this application for determining the stated value.

[0048] All ranges disclosed herein are inclusive of the stated endpoints and are independently combinable.

[0049] The terms "about" and "approximately" can be used to include any numerical value that can be varied without changing the basic function of the value. When used with a range, "about" and "approximately" also disclose the range defined by the absolute values ​​of the two endpoints, for example, "about 2 to about 4" also discloses a range of "2 to 4". Generally, the terms "about" and "approximately" can refer to ±10% of the indicated number. However, for temperature, the term "approximately" refers to ±1°C.

[0050] Example

[0051] Examples 1-5

[0052] Examples 1-5 respectively provide a mouse DIO model and a method for constructing the same.

[0053] The difference between the above embodiments is that the dosage of each component in the high-calorie feed is different, as shown in Table 1.

[0054] The method for constructing the mouse DIO model in the above embodiment is: giving the experimental mice a high-calorie diet to induce the establishment of the mouse DIO model;

[0055] A total of 10 C57BL / 6 mice (weight 21 ± 2 g) of similar size and weight, aged 6 to 8 weeks were selected;

[0056] The mice were placed in a standard feeding environment for one week and given a normal diet. The specific methods of the mice's adaptation steps in the feeding environment are as follows: provide a stable feeding environment, and the temperature, humidity, light and other conditions must meet the survival needs of the mice. Keep the feeding environment clean and hygienic, change the bedding regularly to prevent the spread of diseases, and avoid environmental noise and interference to reduce the stress response of the mice.

[0057] After the adaptation period, high-calorie feed (as shown in Table 1, each raw material component was mixed according to the corresponding weight portion) was given to induce the establishment of the DIO model, and sufficient drinking water was provided.

[0058] The mice were fed a high-calorie diet for 7-12 weeks, and their body weight and body fat content were measured every week. When the mice's body weight increased by about 25% compared to their initial weight and their body fat content increased to about 40%, the DIO model was successfully established.

[0059] Table 1 Amount of each component in high calorie feed in Examples 1-5 and Comparative Examples 1-5

[0060] Comparative Example

[0061] Comparative Examples 1-5

[0062] Comparative Examples 1-5 respectively provide a mouse DIO model and a method for constructing the same.

[0063] The difference between the comparative example and Example 1 is that the dosage of each component in the high-calorie feed is different, as shown in Table 1.

[0064] The remaining parameters of the comparative example and embodiment 1 are the same.

[0065] Performance testing

[0066] (1) The experimental method in Examples 1-5 and Comparative Examples 1-5 is to continue to give high-calorie feed for 8-12 weeks, and measure the body weight and body fat content of mice at 3 weeks and 6 weeks of the experiment and every week thereafter. When the body weight of the mice increases by about 25% compared with the initial body weight and the body fat content increases to about 40%, it is determined that the DIO model is successfully established.

[0067] The results of the mouse body weight test are shown in Table 2.

[0068] Table 2 Body weight test results of mice in Examples 1-5 and Comparative Examples 1-5

[0069]

[0070] Combined with Table 2, by comparing the test results of Examples 1-5 with Comparative Examples 1-5, it can be seen that compared with the commercially available high-fat feed in Example 1 (the test period reaches 10 weeks), or the combination of high-fat feed, white bean extract, pomegranate peel polyphenols, and phaseol, or the combination of high-fat feed, honeysuckle vine extract, tea polyphenols, and phaseol, or the combination of high-fat feed, honeysuckle vine extract, pomegranate peel polyphenols, and quercetin; the high-calorie feed provided in Examples 2-6 of the present application (combined use of high-fat feed, honeysuckle vine extract, pomegranate peel polyphenols, and phaseol) is used to construct a mouse DIO model, which has the effect of rapidly increasing mouse obesity, thereby more quickly establishing a mouse DIO model. The above results show that the high-calorie feed provided in the present application helps the occurrence of obesity caused by excessive energy accumulation in experimental mice, increases the mouse obesity index, shortens the cycle of constructing a mouse DIO model, and thus helps to reduce the cost of the experiment.

[0071] (2) Mouse serum biochemical indices: After the mouse DIO model was successfully established, the changes in serum biochemical indices of the experimental mice in Example 1, Comparative Example 1 and the control group were measured.

[0072] The test results are shown in Table 3.

[0073] Table 3 Serum biochemical index test results of mice in Example 1, Comparative Example 1 and Control Group

[0074]

[0075] Combined with the results of serum biochemical test indicators of experimental mice in Table 3, it can be seen that after the mouse DIO model in Example 1 was successfully established, there was no drug toxicity in the liver function and kidney function of the mice, indicating that the high-calorie feed provided in this application for preparing the feed for constructing a mouse obesity model has experimental safety and can be safely used for modeling. Example 6

[0076] Example 6 provides a mouse DIO movement model and a method for constructing the same.

[0077] The method for constructing the mouse DIO movement model in this embodiment is:

[0078] S1: Experimental mice were given a high-calorie diet to induce the establishment of a mouse DIO model;

[0079] S1-1: 15 C57BL / 6 mice of similar size and weight, aged 6 to 8 weeks were selected;

[0080] S1-2: Place the mice in a standard feeding environment for one week and give them a normal diet. The specific steps for the mice to adapt to the feeding environment are as follows: provide a stable feeding environment, and the temperature, humidity, light and other conditions must meet the survival needs of the mice. Keep the feeding environment clean and hygienic, change the bedding regularly to prevent the spread of diseases, and avoid environmental noise and interference to reduce the stress response of the mice.

[0081] S1-3: After the adaptation period, high-calorie feed (prepared in Example 2) was given to induce the establishment of a DIO model, and sufficient drinking water was provided.

[0082] S1-4: The mice were fed a high-calorie diet for 8 weeks, and their body weight and body fat content were measured every week. When the mice's body weight increased by about 25% compared to their initial weight and their body fat content increased to about 40%, the DIO model was successfully established.

[0083] S2: Grouping and exercise intervention of mice, including a running exercise intervention group and a swimming exercise intervention group;

[0084] S2-1: The successfully established DIO mice were randomly divided into an exercise intervention group and a control group;

[0085] S2-2: The mice in the exercise intervention group underwent a specific exercise program, including the exercise intervention group being divided into a running exercise intervention group and a swimming exercise intervention group. The number of mice in each of the control group, the running exercise intervention group, and the swimming exercise intervention group was 5 (numbered 1, 2, 3, 4, and 5, respectively);

[0086] The specific method of the running exercise intervention group is: using a professional mouse treadmill, running training is performed twice a day, each time for 30 minutes, and the speed is gradually increased from the initial 5m / min to 15m / min. During the running process, an appropriate slope is set to increase the intensity of exercise;

[0087] The specific method of the swimming exercise intervention group is: swimming training in a constant temperature swimming tank, three times a week, 20 minutes each time. The water temperature is maintained at 30-32℃ to prevent the mice from hypothermia.

[0088] S2-3: The mice in the control group were maintained in a normal breeding environment without any exercise intervention, and their diet was the same as that of the exercise intervention group.

[0089] S3: perform mouse index detection;

[0090] S3-1: During the exercise intervention, the body weight, body fat content, blood sugar, and blood lipid indicators of mice were regularly tested, and the testing time points were set at the 4th, 8th, and 12th week after the start of the exercise intervention.

[0091] S3-2: Body weight measurement: Use an electronic scale to accurately measure the body weight of mice. Each measurement should be performed at the same time to reduce errors.

[0092] S3-3: Body fat content detection: Dual-energy X-ray absorptiometry (DEXA) or magnetic resonance imaging (MRI) technology was used to measure the body fat content of mice and evaluate the fat distribution.

[0093] S3-4: Blood glucose and blood lipid testing: The blood of mice was collected by tail tip blood sampling method, and blood glucose and blood lipid levels were tested using a blood glucose meter and a blood lipid tester, respectively, including fasting blood glucose, postprandial blood glucose, total cholesterol, triglycerides, high-density lipoprotein cholesterol and low-density lipoprotein cholesterol.

[0094] S4: Data analysis;

[0095] S4-1: Statistical analysis was performed on various indicators of mice in the exercise intervention group and the control group, and the differences between the two groups were compared. Statistical methods such as t-test and variance analysis were used to determine the therapeutic effect of exercise intervention.

[0096] S4-2: Analyze the therapeutic effect of exercise intervention on the mouse DIO model, including weight loss, body fat reduction, lower blood sugar and blood lipid levels, etc., draw indicator change curves, and intuitively show the changing trend of the effect of exercise intervention over time.

[0097] S4-3: Based on literature and existing research results, we will explore in depth the mechanism of action of exercise intervention to provide a theoretical basis for further research and clinical application.

[0098] The body weight, body fat content, blood sugar and blood lipid levels of mice at different test cycles in Example 6 were measured, and the results are shown in Table 4 below.

[0099] Table 4 Results of body weight, body fat content, blood sugar and blood lipid test of mice in different test cycles

[0100]

[0101] In summary, it can be clearly seen from Table 4 that the DIO exercise model of mice was constructed after the exercise intervention method based on the mouse DIO model, showing significant effects. After the specific exercise intervention, the weight gain of the mice in the running exercise intervention group and the swimming exercise intervention group was gradually reduced compared with the initial weight, and the body fat content was also significantly reduced. At the same time, the blood sugar and blood lipid levels also gradually decreased with the extension of the exercise intervention time, which shows that exercise intervention can effectively improve the glucose and lipid metabolism of mice and reduce the risk of diabetes and cardiovascular diseases. The indicators of the mice in the control group continued to deteriorate, highlighting the importance of exercise intervention. When the modeling period was 14-16 weeks, the experimental mice in the running exercise intervention group had stable indicators, and the DIO exercise model of mice was constructed; when the modeling period was 12-14 weeks, the experimental mice in the swimming exercise intervention group had stable indicators, and the DIO exercise model of mice was constructed, so as to better provide a strong reference and reference for the treatment of human obesity and related diseases.

[0102] Although the present invention has been described in detail above with general descriptions and specific embodiments, it is obvious to those skilled in the art that some modifications or improvements may be made thereto based on the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention all fall within the scope of protection claimed by the present invention.

Claims

1. A method for constructing a mouse DIO movement model, characterized in that: The specific steps include: S1: Experimental mice were given a high-calorie diet to induce the establishment of a mouse DIO model; The high-calorie feed formula specifically includes the following components in parts by weight: 96-98 parts of high-fat feed, 2-4 parts of honeysuckle vine extract, 0.4-0.8 parts of pomegranate peel polyphenols, and 0.1-0.3 parts of phaseolin; S2: performing grouping and exercise intervention on mice; the exercise intervention includes an exercise mode combining a running exercise intervention group and a swimming exercise intervention group; The specific method of the running exercise intervention group program is: using a professional mouse treadmill, running training is performed twice a day, each time for 26-34 minutes, the speed is gradually increased from the initial 3-7m / min to 13-17m / min, and the slope is set during the running process to increase the exercise intensity; The specific method of the swimming exercise intervention group program is: swimming training in a constant temperature swimming tank, 2-4 times a week, each time for 15-25 minutes, and the water temperature is maintained at 30-32°C; S3: mouse index detection; S4: Data analysis to obtain a mouse DIO exercise model; the modeling period of the mouse DIO exercise model is 12-16 weeks; when various detection indicators of the experimental mice reach a plateau, the mouse DIO exercise model is constructed.

2. The method for constructing a mouse DIO movement model according to claim 1, characterized in that: The specific steps of S1 are: S1-1: Select C57BL / 6 mice of similar size and weight, 6 to 8 weeks old, as experimental mice; S1-2: The experimental mice were placed in a standard feeding environment for one week and given a normal diet; S1-3: After the adaptation period, high-calorie diet was given to induce the DIO model, and sufficient drinking water was provided; S1-4: Continue to give high-calorie diet for 7-12 weeks, measure the weight and body fat content of mice every week, and when the weight of mice increases by 25% compared with the initial weight and the body fat content increases to 40%, it is determined that the mouse DIO model is successfully established.

3. The method for constructing a mouse DIO movement model according to claim 2, characterized in that: The high-calorie feed formula specifically includes the following components in parts by weight: 97 parts of high-fat feed, 3 parts of honeysuckle vine extract, 0.6 parts of pomegranate peel polyphenols, and 0.2 parts of phaseolin.

4. The method for constructing a mouse DIO movement model according to claim 2, characterized in that: The specific method of placing the experimental mice in the breeding environment for adaptation in S1-2 is: Provide a stable breeding environment. The temperature, humidity, and lighting conditions must meet the survival needs of mice. Keep the breeding environment clean and sanitary. Change the bedding regularly to prevent the spread of disease. Avoid environmental noise and interference to reduce the stress response of mice.

5. The method for constructing a mouse DIO movement model according to claim 1, characterized in that: The specific method for grouping mice and exercising intervention in S3 is: S2-1: The successfully established DIO mice were randomly divided into an exercise intervention group and a control group; S2-2: The mice in the exercise intervention group underwent an exercise program, including a combined exercise pattern of the running exercise intervention group and the swimming exercise intervention group; S2-3: The mice in the control group were maintained in a normal breeding environment without any exercise intervention, and their diet was the same as that of the exercise intervention group.

6. The method for constructing a mouse DIO movement model according to claim 1, characterized in that: The specific method of the running exercise intervention group program is: using a professional mouse treadmill, running training is performed twice a day, each time for 28-32 minutes, the speed is gradually increased from the initial 4-6m / min to 14-16m / min, and an appropriate slope is set during the running process to increase the exercise intensity; The specific method of the swimming exercise intervention group program is: swimming training is performed in a constant temperature swimming tank 2-4 times a week, each time for 18-22 minutes, and the water temperature is maintained at 30-32°C to avoid hypothermia of the mice.

7. The method for constructing a mouse DIO movement model according to claim 6, characterized in that: The specific method of the running exercise intervention group program is: using a professional mouse treadmill, running training is performed twice a day, each time for 28-32 minutes, the speed is gradually increased from the initial 5m / min to 15m / min, and an appropriate slope is set during the running process to increase the exercise intensity; The specific method of the swimming exercise intervention group program is: swimming training is performed in a constant temperature swimming tank 3 times a week, each time for 20 minutes, and the water temperature is maintained at 30-32°C to avoid hypothermia of the mice.

8. The method for constructing a mouse DIO movement model according to claim 1, characterized in that: The specific method for performing mouse index detection is as follows: S3-1: During the exercise intervention, the body weight, body fat content, blood sugar, and blood lipid indexes of mice were regularly tested. The testing time points were set at 2, 4, 6, 8, 10, 12, 14, and 16 weeks after the start of the exercise intervention. S3-2: Body weight measurement: Use an electronic scale to accurately measure the body weight of mice. Each measurement should be performed at the same time to reduce errors. S3-3: Body fat content detection: Dual-energy X-ray absorptiometry or magnetic resonance imaging was used to measure the body fat content of mice and evaluate the fat distribution; S3-4: Blood glucose and blood lipid testing: The blood of mice was collected by tail tip blood sampling method, and blood glucose and blood lipid levels were tested using a blood glucose meter and a blood lipid tester, respectively, including fasting blood glucose, postprandial blood glucose, total cholesterol, triglycerides, high-density lipoprotein cholesterol and low-density lipoprotein cholesterol.

9. The method for constructing a mouse DIO movement model according to claim 1, characterized in that: The specific method in the data analysis step is as follows: S4-1: Statistical analysis was performed on various indicators of mice in the exercise intervention group and the control group, and the differences between the two groups were compared. The statistical methods of t-test or variance analysis were used to determine the effect of exercise intervention; S4-2: Analyze the effects of exercise intervention on the DIO exercise model of mice, including weight loss, body fat reduction, and lower blood sugar and blood lipid levels, and intuitively display the changing trend of the effect of exercise intervention over time; the modeling period of the DIO exercise model of mice is 12-16 weeks; when the various detection indicators of the experimental mice reach a plateau, the DIO exercise model of mice is established; S4-3: Based on the literature and existing research results, further explore the mechanism of action of exercise intervention.

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