Natural mixed feed additive and preparation method thereof

Abstract

The invention relates to the technical field of feed formulas, and particularly discloses a natural mixed feed additive and a preparation method thereof. The natural mixed feed additive comprises the following raw materials in parts by mass: 4-7 parts of vitamin E, 5-15 parts of beta-carotene, 10-16 parts of soybean oil, 20-35 parts of deionized water, 5-8 parts of modified probiotic microcapsules and 1-5 parts of an emulsifier, wherein the modified probiotic microcapsules contain sodium alginate-pectin composite microencapsulated probiotics; the preparation method comprises the following steps: heating 10-16 parts of soybean oil to 35-45 DEG C, then adding 4-7 parts of vitamin E and 5-15 parts of beta-carotene, and stirring; adding 1-5 parts of an emulsifier into 20-35 parts of deionized water, heating to 45-55 DEG C, stirring, and then slowly adding into the mixed solution obtained in the step S1; 5-8 parts of modified probiotic microcapsules are added, and stirring is conducted for 8-12 min under the condition of 100-200 r / min. The natural mixed feed additive disclosed by the invention has the advantage that the defect that the feed additive still has defects in the aspect of reducing intestinal diseases of animals can be overcome.

Description

Technical Field

[0001] This application relates to the technical field of feed formulation, and more specifically, to a natural mixed feed additive and a preparation method thereof. Background Art

[0002] As the material basis for the development of the aquaculture industry, with the improvement of the intensification degree of the aquaculture industry, the dependence on feed has gradually increased. In order to meet the needs of animal growth and development for nutritional components and physicochemical balance substances, a small amount of additional substances, namely feed additives, are added in feed production. Feed additives can balance the nutritional components of feed, promote animal growth, prevent the occurrence of diseases, etc. At the same time, the development of the feed safety-related industry faces many new challenges. In recent years, the state has been more strict and standardized in the management and use of feed additives, the threshold has been continuously increased, and the new requirements have also become the driving force for the further development of China's feed additive industry.

[0003] Animals are generally weaned 3 - 4 weeks after birth. For example, piglets. Early weaning makes the underdeveloped intestines prematurely exposed to indigestible solid diets and are transferred to a new environment for feeding. Piglets will have post-weaning stress reactions, causing intestinal oxidative stress and intestinal flora imbalance, which will lead to intestinal damage and persistent inflammation. Intestinal damage and inflammation are the main reasons for causing piglet diarrhea and damaging the growth performance of piglets. In order to prevent piglet post-weaning diarrhea, adding antibiotic feed additives to the diets of weaned piglets has become one of the conventional means in aquaculture. In recent years, the problem of pathogenic bacteria's resistance to antibiotics has been intensifying, and at the same time, the widespread use of antibiotics in aquaculture has caused serious pollution to the environment. Relevant policies have been introduced in most regions of the world to strictly restrict the use of antibiotics in aquaculture. Therefore, there is an urgent need for a feed additive that can replace antibiotics to reduce animal intestinal diseases. Summary of the Invention

[0004] In order to improve the deficiencies of feed additives in reducing animal intestinal diseases, this application provides a natural mixed feed additive and a preparation method thereof.

[0005] A natural mixed feed additive and a preparation method thereof provided by this application adopt the following technical solutions:

[0006] In the first aspect, a natural mixed feed additive provided by this application comprises the following raw materials in parts by mass: 4 - 7 parts of vitamin E, 5 - 15 parts of β-carotene, 10 - 16 parts of soybean oil, 20 - 35 parts of deionized water, 5 - 8 parts of modified probiotic microcapsules, and 1 - 5 parts of emulsifier. The modified probiotic microcapsules contain sodium alginate-pectin composite microencapsulated probiotics.

[0007] The long-term use of antibiotics in livestock and poultry feed can increase the number of drug-resistant strains of pathogenic bacteria, and normal strains will also develop drug resistance, thus affecting the therapeutic effect of drugs. Moreover, drug-resistant bacteria can be transmitted to the human body through the food chain, threatening the control of human diseases. At the same time, antibiotics can enter the environment through animal excretions, thus disrupting the ecological balance and polluting water sources.

[0008] Vitamin E can protect intestinal cells from free radical damage by neutralizing free radicals, maintaining the integrity of the intestinal mucosa. A healthy intestinal mucosa can serve as a physical barrier, making it difficult for pathogenic bacteria to invade, thereby reducing the risk of intestinal infections. Vitamin E can regulate the immune function of animals, enhance the local immune response in the intestine, and improve the resistance to intestinal pathogens. β-carotene can be converted into vitamin A in animals, which can promote the regeneration and repair of intestinal mucosal epithelial cells and maintain the integrity of the intestinal barrier. Intact intestinal epithelial cells can better resist the invasion of pathogens and reduce the occurrence of intestinal diseases. Moreover, β-carotene has certain antioxidant and immunomodulatory effects, which can enhance the immune function of the intestine, promote the growth of beneficial intestinal microorganisms, and also inhibit the release of pro-inflammatory factors and reduce intestinal inflammation. Soybean oil provides energy and essential fatty acids for animals, promotes the stability of the intestinal cell membrane structure, and also helps with the absorption and utilization of vitamin E and β-carotene.

[0009] At the same time, probiotics can colonize in the intestine, compete with harmful bacteria for nutrients and living space, thereby reducing the growth and reproduction of harmful bacteria and regulating the balance of the intestinal flora. Probiotics can also produce some beneficial metabolites, such as organic acids, bacteriocins, etc. These substances can lower the intestinal pH value, inhibit the growth of pathogenic bacteria, and also have a certain antibacterial effect. The structure of the microcapsule can protect probiotics from being easily destroyed when passing through harsh environments such as gastric acid and bile, ensuring that probiotics can reach the intestine smoothly and play their roles. At the same time, during processing and storage, vitamin E can neutralize free radicals, reduce the damage of oxidative stress to probiotics, and maintain their activity. Probiotics activate intestinal-related lymphoid tissues, promote the production of secretory immunoglobulin A, and jointly build a systemic immune defense with vitamin E.

[0010] Preferably, the modified probiotic microcapsule comprises the following raw materials in parts by mass: 1-2 parts of Bifidobacterium and 3-5 parts of alginate-pectin composite microencapsulated probiotics.

[0011] Since Bifidobacterium can reduce the intestinal pH value by producing organic acids, thereby reducing the growth and reproduction of harmful bacteria such as Escherichia coli and Salmonella, making it difficult for harmful bacteria to colonize in the intestine, thus reducing the risk of intestinal infections. Alginate-pectin composite microencapsulated probiotics can increase the number of beneficial bacteria, synergistically optimize the intestinal flora structure, and enhance the stability and health of the intestinal microecology.

[0012] Preferably, the preparation method of the modified probiotic microcapsules is as follows: Activate 1-2 parts of Bifidobacterium in liquid MRS medium at 35-39 °C for 16-20 h, and put 3-5 parts of alginate-pectin complex microencapsulated probiotics into MRS medium for secondary fermentation to obtain modified probiotic microcapsules.

[0013] Due to the appropriate temperature and culture time, Bifidobacterium can grow and reproduce sufficiently, and its metabolic activity is enhanced. Thus, it can quickly play a role after entering the animal intestine, better regulate the balance of intestinal flora, inhibit the growth of harmful bacteria. At the same time, secondary fermentation of the alginate-pectin complex microencapsulated probiotics enables the probiotics inside the microcapsules to obtain more sufficient nutrients, promoting their growth and metabolism. During the secondary fermentation process, Bifidobacterium and the alginate-pectin complex microencapsulated probiotics can interact. Bifidobacterium can utilize the relatively stable microenvironment created by the microencapsulated probiotics to better exert its own functions, and the microencapsulated probiotics can obtain more favorable survival conditions due to the metabolic products of Bifidobacterium. The two work synergistically to enhance the improvement effect on animal intestinal health.

[0014] Preferably, the alginate-pectin complex microencapsulated probiotics include the following raw materials: 2-4 ml of alginate-pectin complex solution, 1-3 ml of concentrated bacterial solution, 0.3-0.7 g of calcium carbonate, 6-14 ml of deionized water, 15-25 ml of liquid paraffin, 0.5-1.5 ml of glacial acetic acid.

[0015] Since both alginate and pectin are natural high-molecular polymers, the composite solution formed by the two has good film-forming properties and biocompatibility, and can form a protective film on the surface of probiotics, making them not easily eroded by the external adverse environment, such as gastric acid and bile, enabling the probiotics to reach the intestine smoothly and play a role. At the same time, this composite film can control the release rate of probiotics, making them slowly release in the intestine and prolonging the action time. The concentrated bacterial solution provides a sufficient number of probiotics for microencapsulation, enabling them to have sufficient active bacteria in the animal intestine to play roles such as regulating the balance of intestinal flora, reducing the growth of harmful bacteria, and enhancing intestinal immunity, improving the efficacy of the microencapsulated probiotics.

[0016] Preferably, the preparation method of the alginate-pectin complex microencapsulated probiotics is as follows: Weigh 0.3-0.7 g of calcium carbonate and dissolve it in 6-14 ml of deionized water, stir it evenly with 2-4 ml of alginate-pectin complex solution, then add 1-3 ml of concentrated bacterial solution and mix evenly. Subsequently, disperse it in 15-25 ml of liquid paraffin and stir at 350-450 r / min for 4-6 min. Then add 0.5-1.5 ml of glacial acetic acid and react for 8-12 min. Finally, rinse it with deionized water 2-4 times to obtain alginate-pectin complex microencapsulated probiotics;

[0017] Among them, the preparation method of the concentrated bacterial liquid: Centrifuge the activated bacterial liquid at 3500 - 4500 r / min for 8 - 12 min, wash it with physiological saline 1 - 3 times, and resuspend the washed bacterial cells in physiological saline to obtain the concentrated bacterial liquid.

[0018] Preferably, the sodium alginate - pectin composite solution comprises the following raw materials: 0.1 - 0.3 g of naringin, 26 - 34 ml of deionized water, 0.3 - 0.5 g of pectin, and 0.6 - 1 g of sodium alginate.

[0019] Due to the biological activities of naringin such as antioxidant, anti - inflammatory, neuroprotective, anti - obesity, and anti - diabetic properties, in the composite solution, its antioxidant property can protect probiotics from oxidative damage, maintain the activity of probiotics, and at the same time has effective broad - spectrum antibacterial activity, showing antibacterial effects against bacteria such as Escherichia coli, Staphylococcus aureus, and Listeria innocua, as well as different viruses and fungi, which helps to inhibit the growth of harmful microorganisms in feed and further protect the intestinal health of animals.

[0020] Preferably, the preparation method of the sodium alginate - pectin composite solution: Weigh 0.1 - 0.3 g of naringin and add it to 18 - 22 ml of deionized water, magnetically stir at 55 - 65 °C and 350 - 450 r / min for 25 - 35 min, then add 0.3 - 0.5 g of pectin and continue stirring for 0.5 - 1.5 h to obtain a mixed solution. Separately, take 0.6 - 1 g of sodium alginate and add it to 8 - 12 ml of deionized water, magnetically stir at 20 - 30 °C and 350 - 450 r / min for 0.5 - 1.5 h for dissolution, then transfer the dissolved sodium alginate solution to the mixed solution, continue stirring at 350 - 450 r / min for 0.5 - 1.5 h, then quickly cool in a water bath at 15 - 25 °C for 25 - 35 min, and ultrasonically remove the bubbles in the solution.

[0021] In the second aspect, the present application provides a preparation method of a natural mixed feed additive, adopting the following technical scheme:

[0022] A preparation method of a natural mixed feed additive, comprising the following steps:

[0023] S1: Heat 10 - 16 parts of soybean oil to 35 - 45 °C, then add 4 - 7 parts of vitamin E and 5 - 15 parts of β - carotene, and stir.

[0024] S2: Add 1 - 5 parts of emulsifier to 20 - 35 parts of deionized water, heat to 45 - 55 °C and stir, then slowly add it to the mixed liquid obtained in S1.

[0025] S3: Add 5 - 8 parts of modified probiotic microcapsules and stir for 8 - 12 min under the condition of 100 - 200 r / min.

[0026] In summary, the present application has the following beneficial effects:

[0027] 1. Vitamin E can protect intestinal cells from free radical damage by neutralizing free radicals, maintaining the integrity of the intestinal mucosa. A healthy intestinal mucosa can serve as a physical barrier, making it difficult for pathogenic bacteria to invade, thereby reducing the risk of intestinal infection. Vitamin E can regulate the immune function of animals, enhance the local immune response in the intestine, and improve the resistance to intestinal pathogens. β - carotene can be converted into vitamin A in animals, which can promote the regeneration and repair of intestinal mucosal epithelial cells and maintain the integrity of the intestinal barrier. Intact intestinal epithelial cells can better resist the invasion of pathogens and reduce the occurrence of intestinal diseases. Moreover, β - carotene has certain antioxidant and immunomodulatory effects, which can enhance the immune function of the intestine, promote the growth of beneficial intestinal microorganisms, and also inhibit the release of pro - inflammatory factors and reduce intestinal inflammation. Soybean oil provides energy and essential fatty acids for animals, promotes the stability of the intestinal cell membrane structure, and also helps with the absorption and utilization of vitamin E and β - carotene.

[0028] At the same time, probiotics can colonize in the intestine, compete with harmful bacteria for nutrients and living space, thereby reducing the growth and reproduction of harmful bacteria and regulating the balance of the intestinal flora. Probiotics can also produce some beneficial metabolites, such as organic acids, bacteriocins, etc. These substances can lower the intestinal pH value, inhibit the growth of pathogenic bacteria, and also have a certain antibacterial effect. The structure of the microcapsule can protect probiotics from being easily destroyed when passing through harsh environments such as gastric acid and bile, ensuring that probiotics can reach the intestine smoothly and play their roles. At the same time, during processing and storage, vitamin E can reduce the damage of oxidative stress to probiotics by neutralizing free radicals and maintain their activity. Probiotics promote the generation of secretory immunoglobulin A by activating intestinal - related lymphoid tissues and jointly build a systemic immune defense with vitamin E.

[0029] 2. Since Bifidobacterium can reduce the intestinal pH value by producing organic acids, thereby reducing the growth and reproduction of harmful bacteria such as Escherichia coli and Salmonella, making it difficult for harmful bacteria to colonize in the intestine, thus reducing the risk of intestinal infection. Alginate - pectin complex microencapsulated probiotics can increase the number of beneficial bacteria, synergistically optimize the intestinal flora structure, and enhance the stability and health of the intestinal microecosystem.

[0030] 3. Since appropriate temperature and culture time can allow Bifidobacterium to grow and reproduce sufficiently and enhance its metabolic activity, it can quickly play a role after entering the animal intestine, better regulate the balance of intestinal flora, inhibit the growth of harmful bacteria, and at the same time perform secondary fermentation on alginate-pectin complex microencapsulated probiotics, enabling the probiotics inside the microcapsules to obtain more sufficient nutrients, promoting their growth and metabolism. During the secondary fermentation process, Bifidobacterium and alginate-pectin complex microencapsulated probiotics can interact. Bifidobacterium can utilize the relatively stable microenvironment created by the microencapsulated probiotics to better exert its own functions, and the microencapsulated probiotics can obtain more favorable survival conditions due to the metabolic products of Bifidobacterium. The two work synergistically to enhance the improvement effect on animal intestinal health. Detailed implementation manners

[0031] The following further elaborates on this application in conjunction with Examples 1-10 and Comparative Examples 1-2.

[0032] Raw materials

[0033] Vitamin E CAS: 2074-53-5; β-carotene CAS: 301150-50-5; soybean oil CAS: 8001-22-7; deionized water CAS: 7732-18-5; emulsifier lecithin CAS: 8002-43-5; Bifidobacterium, Shanghai Jiadeer Chemical Technology Co., Ltd.; calcium carbonate CAS: 471-34-1; liquid paraffin CAS: 8042-47-5; glacial acetic acid CAS: 64-19-7; naringin CAS: 10236-47-2; pectin, Huaibei Kele Biotechnology Co., Ltd.; sodium alginate, Lianyungang Tiantian Seaweed Industry Co., Ltd.

[0034] Example 1

[0035] A natural mixed feed additive, comprising the following raw materials: 5.5 g of vitamin E, 10 g of β-carotene, 13 ml of soybean oil, 27.5 ml of deionized water, 6.5 g of modified probiotic microcapsules, and 3 g of lecithin.

[0036] Specifically, the preparation method of the natural mixed feed additive includes the following steps:

[0037] S1: Activate 1.5 ml of Bifidobacterium in liquid MRS medium at 37 °C for 18 h.

[0038] S2: Weigh 0.2 g of naringin and add it to 20 ml of deionized water. Stir magnetically at 60 °C and 400 r / min for 30 min. Then add 0.4 g of pectin and continue stirring for 1 h to obtain a mixed solution. Separately, weigh 0.8 g of sodium alginate and add it to 10 ml of deionized water. Stir magnetically at 25 °C and 400 r / min for 1 h to dissolve it. Then transfer the dissolved sodium alginate solution to the mixed solution and continue stirring at 400 r / min for 1 h. Subsequently, quickly cool it in a 20 °C water bath for 30 min and ultrasonically remove the bubbles in the solution to obtain a sodium alginate-pectin composite solution;

[0039] S3: Weigh 0.5 g of calcium carbonate and dissolve it in 10 ml of deionized water. Stir it evenly with 3 ml of the sodium alginate-pectin composite solution. Then add 2 ml of the concentrated bacterial solution and mix evenly. Subsequently, disperse it into 20 ml of liquid paraffin and stir at 400 r / min for 5 min. Then add 1 ml of glacial acetic acid and react for 10 min. Finally, rinse it 3 times with deionized water to obtain sodium alginate-pectin composite microencapsulated probiotics;

[0040] Among them, the preparation method of the concentrated bacterial solution: Centrifuge the activated bacterial solution at 4000 r / min for 10 min, wash it 2 times with physiological saline, and resuspend the washed bacterial cells in physiological saline to obtain the concentrated bacterial solution;

[0041] S4: Put 4 g of sodium alginate-pectin composite microencapsulated probiotics into MRS medium for secondary fermentation to obtain modified probiotic microcapsules;

[0042] S5: Heat 13 ml of soybean oil to 35 - 45 °C, then add 5.5 g of vitamin E and 10 g of β-carotene and stir;

[0043] S6: Add 3 g of lecithin to 27.5 ml of deionized water, heat it to 50 °C and stir, then slowly add it to the mixed solution obtained in S5;

[0044] S7: Add 6.5 g of modified probiotic microcapsules and stir at 150 r / min for 10 min.

[0045] Example 2 - Example 3

[0046] The difference from Example 1 is that the addition amounts of the components of the natural mixed feed additive are different, as shown in Table 1 specifically.

[0047] Table 1 Component addition amounts of the natural mixed feed additive in Examples 1 - 3

[0048] Example 1 Example 2 Example 3 Vitamin E 5.5g 4g 7g β-Carotene 10g 15g 5g Soybean oil 13ml 10ml 16ml Deionized water 27.5ml 20ml 35ml Modified probiotic microcapsules 6.5g 8g 5g Lecithin 3g 5g 1g

[0049] Example 4

[0050] The difference from Example 1 is that the modified probiotic microcapsules are replaced with probiotics of the same added amount.

[0051] Examples 5 - 6

[0052] The difference from Example 1 is that the added amounts of the components of the modified probiotic microcapsules are different, as specifically shown in Table 2.

[0053] Table 2 Added amount table of each component of the modified probiotic microcapsules in Example 1 and Examples 5 - 6

[0054] Example 1 Example 5 Example 6 Bifidobacterium 1.5ml 1ml 2ml Sodium alginate-pectin complex microencapsulated probiotics 4g 5g 3g

[0055] Examples 7 - 8

[0056] The difference from Example 1 is that the added amounts of the components of the sodium alginate - pectin complex microencapsulated probiotics are different, as specifically shown in Table 3.

[0057] Table 3 Added amount table of each component of the sodium alginate - pectin complex microencapsulated probiotics in Example 1 and Examples 7 - 8

[0058] Example 1 Example 7 Example 8 Sodium alginate-pectin composite solution 3ml 4ml 2ml Concentrated bacterial solution 2ml 3ml 1ml Calcium carbonate 0.5g 0.3g 0.7g Deionized water 10ml 6ml 14ml Liquid paraffin 20ml 15ml 25ml Glacial acetic acid 1ml 1.5ml 0.5ml

[0059] Examples 9 - 10

[0060] The difference from Example 1 is that the added amounts of the components of the sodium alginate - pectin complex solution are different, as specifically shown in Table 4.

[0061] Table 4 Added amount table of each component of the sodium alginate - pectin complex solution in Example 1 and Examples 9 - 10

[0062] Example 1 Example 9 Example 10 Naringin 0.2g 0.1g 0.3g Deionized water 30ml 34ml 26ml Pectin 0.4g 0.3g 0.5g Sodium alginate 0.8g 1g 0.6g

[0063] Comparative Example 1

[0064] The difference from Example 1 is that the modified probiotic microcapsules are replaced with antibiotics of the same added amount.

[0065] Comparative Example 2

[0066] The difference from Example 1 is that vitamin E is no longer added.

[0067] Performance detection test

[0068] I. Growth performance test

[0069] Select 220 healthy weaned piglets at 35 days old with similar birth dates and weights, and divide them into 11 groups evenly, with 20 piglets in each group. Add the natural mixed feed additives prepared in Examples 1 - 10 and Comparative Example 1 to the feeding diets of the 11 groups. The addition amount of the natural feed additive is 1.8% of the weight of the piglet diet. Feed continuously for 30 days, with 6 feedings per day, 2 times each in the morning, noon, and evening.

[0070] Before the experiment, record the morning fasting net weight of each group of piglets, and use the average weight as the initial weight. After the experiment, record the morning fasting net weight of the piglets, and use the average weight as the final weight. At the same time, record the diarrhea rate of the health status of the piglets and take the average value. The experimental results are shown in Table 5.

[0071] Among them, the diarrhea rate = (the cumulative diarrhea days of diarrhea piglets / the cumulative feeding days of the whole group of piglets) × 100%.

[0072] Table 5 Growth performance test table of Examples 1 - 10 and Comparative Examples 1 - 2

[0073]

[0074]

[0075] Combined with Example 1 and Comparative Example 1 and Table 5, it can be seen that compared with Example 1, although the average daily gain of Comparative Example 1 has increased, the diarrhea rate of Comparative Example 1 has increased significantly. This shows that compared with adding antibiotics, adding modified probiotic microcapsules can effectively reduce the diarrhea rate of piglets, thereby improving the intestinal health of piglets.

[0076] The reason is that probiotics can colonize in the intestine, compete with harmful bacteria for nutrients and living space, thereby reducing the growth and reproduction of harmful bacteria and regulating the balance of the intestinal flora. Probiotics can also produce some beneficial metabolites, such as organic acids, bacteriocins, etc. These substances can reduce the intestinal pH value, inhibit the growth of pathogenic bacteria, and at the same time have a certain antibacterial effect. The structure of the microcapsule can protect the probiotics from being easily destroyed when passing through harsh environments such as gastric acid and bile, ensuring that the probiotics can reach the intestine smoothly and play a role.

[0077] Bifidobacterium can reduce the intestinal pH value by producing organic acids, thereby reducing the growth and reproduction of harmful bacteria such as Escherichia coli and Salmonella, making it difficult for harmful bacteria to colonize in the intestine, thus reducing the risk of intestinal infection. Alginate - pectin complex microencapsulated probiotics can increase the number of beneficial bacteria, synergistically optimize the intestinal flora structure, and enhance the stability and health of the intestinal microecology.

[0078] Combined with Example 1 and Comparative Example 2 and Table 5, it can be seen that compared with Example 1, the average daily weight gain of Comparative Example 2 decreased significantly, and at the same time, the diarrhea rate of Comparative Example 2 increased significantly. Thus, it shows that compared with not adding vitamin E, adding vitamin E can effectively reduce the diarrhea rate of piglets, thereby improving the intestinal health of piglets.

[0079] The reason is that vitamin E can protect intestinal cells from free radical damage by neutralizing free radicals, maintaining the integrity of the intestinal mucosa. A healthy intestinal mucosa can serve as a physical barrier, making it difficult for pathogenic bacteria to invade, thereby reducing the risk of intestinal infection. Vitamin E can regulate the immune function of animals, enhance the local immune response in the intestine, and improve the resistance to intestinal pathogens. At the same time, during processing and storage, vitamin E can reduce the damage of oxidative stress to probiotics by neutralizing free radicals and maintain their activity. Probiotics promote the generation of secretory immunoglobulin A by activating intestinal-related lymphoid tissues, and jointly build a systemic immune defense with vitamin E.

[0080] Combined with Example 1 and Examples 2 - 3 and Table 5, it can be seen that compared with Example 1, the average daily weight gain of Examples 2 and 3 decreased, and the diarrhea rate of Examples 2 and 3 increased. Thus, it shows that the addition amount of each component of the natural mixed feed additive affects the intestinal health of piglets.

[0081] Combined with Example 1 and Example 4 and Table 5, it can be seen that compared with Example 1, the average daily weight gain of Example 4 decreased significantly, and the diarrhea rate of Example 4 also increased significantly. Thus, it shows that compared with adding conventional probiotics, adding modified probiotic microcapsules can effectively improve the intestinal health of piglets.

[0082] Combined with Example 1 and Examples 5 - 6 and Table 5, it can be seen that compared with Example 1, the average daily weight gain of Examples 5 and 6 decreased, and the diarrhea rate of Examples 5 and 6 also increased significantly. Thus, it shows that the addition amount of each component of the modified probiotic microcapsules affects the intestinal health of piglets.

[0083] Combined with Example 1 and Examples 7 - 8 and Table 5, it can be seen that compared with Example 1, the average daily weight gain of Examples 7 and 8 decreased, and the diarrhea rate of Examples 7 and 8 also increased significantly. Thus, it shows that the addition amount of sodium alginate - pectin composite microencapsulated probiotics affects the intestinal health of piglets.

[0084] Combined with Example 1 and Examples 9 - 10 and in combination with Table 5, it can be seen that compared with Example 1, the average daily gain of Examples 9 and 10 has decreased, and the diarrhea rate of Examples 9 and 10 has also increased significantly. Thus, it shows that the addition amount of the sodium alginate - pectin composite solution affects the intestinal health of piglets.

[0085] This specific embodiment is only an interpretation of the present application, and it is not a limitation to the present application. After reading this specification, those skilled in the art can make modifications to this embodiment without creative contributions as needed, but as long as it is within the scope of the claims of the present application, it is protected by the patent law.

Claims

1. A natural mixed feed additive, characterized in that, It includes the following raw materials in parts by mass: 4 - 7 parts of vitamin E, 5 - 15 parts of β - carotene, 10 - 16 parts of soybean oil, 20 - 35 parts of deionized water, 5 - 8 parts of modified probiotic microcapsules, and 1 - 5 parts of emulsifier. The modified probiotic microcapsules contain sodium alginate - pectin complex microencapsulated probiotics.

2. The natural mixed feed additive according to claim 1, characterized in that The modified probiotic microcapsules include the following raw materials in parts by mass: 1 - 2 parts of Bifidobacterium, 3 - 5 parts of sodium alginate - pectin complex microencapsulated probiotics.

3. The natural mixed feed additive according to claim 2, wherein The preparation method of the modified probiotic microcapsules: Activate 1 - 2 parts of Bifidobacterium in liquid MRS medium at 35 - 39 °C for 16 - 20 h, and put 3 - 5 parts of sodium alginate - pectin complex microencapsulated probiotics into MRS medium for secondary fermentation to obtain the modified probiotic microcapsules.

4. The natural mixed feed additive according to claim 3, characterized in that, The sodium alginate - pectin complex microencapsulated probiotics include the following raw materials: 2 - 4 ml of sodium alginate - pectin complex solution, 1 - 3 ml of concentrated bacterial liquid, 0.3 - 0.7 g of calcium carbonate, 6 - 14 ml of deionized water, 15 - 25 ml of liquid paraffin, 0.5 - 1.5 ml of glacial acetic acid.

5. A natural mixed feed additive according to claim 4, characterized in that The preparation method of the sodium alginate - pectin complex microencapsulated probiotics: Weigh 0.3 - 0.7 g of calcium carbonate, dissolve it in 6 - 14 ml of deionized water, stir it evenly with 2 - 4 ml of sodium alginate - pectin complex solution, then add 1 - 3 ml of concentrated bacterial liquid and mix evenly. Subsequently, disperse it into 15 - 25 ml of liquid paraffin and stir at 350 - 450 r / min for 4 - 6 min. Then add 0.5 - 1.5 ml of glacial acetic acid and react for 8 - 12 min. Finally, rinse it with deionized water 2 - 4 times to obtain the sodium alginate - pectin complex microencapsulated probiotics. Among them, the preparation method of the concentrated bacterial liquid: Centrifuge the activated bacterial liquid at 3500 - 4500 r / min for 8 - 12 min, wash it with physiological saline 1 - 3 times, and resuspend the washed bacterial cells in physiological saline to obtain the concentrated bacterial liquid.

6. The natural mixed feed additive according to claim 5, characterized in that, The sodium alginate - pectin complex solution includes the following raw materials: 0.1 - 0.3 g of naringin, 26 - 34 ml of deionized water, 0.3 - 0.5 g of pectin, 0.6 - 1 g of sodium alginate.

7. The natural mixed feed additive according to claim 6, characterized in that, The preparation method of the sodium alginate - pectin complex solution: Weigh 0.1 - 0.3 g of naringin and add it to 18 - 22 ml of deionized water, stir magnetically at 55 - 65 °C and 350 - 450 r / min for 25 - 35 min, then add 0.3 - 0.5 g of pectin and continue to stir for 0.5 - 1.5 h to obtain a mixed solution. Separately, weigh 0.6 - 1 g of sodium alginate and add it to 8 - 12 ml of deionized water, stir magnetically at 20 - 30 °C and 350 - 450 r / min for 0.5 - 1.5 h to dissolve it. Then transfer the dissolved sodium alginate solution to the mixed solution and continue to stir at 350 - 450 r / min for 0.5 - 1.5 h. Subsequently, quickly cool it in a water bath at 15 - 25 °C for 25 - 35 min and ultrasonically remove the bubbles in the solution.

8. A method for preparing a natural mixed feed additive according to any one of claims 1-7, characterized in that, It includes the following steps: S1: Heat 10 - 16 parts of soybean oil to 35 - 45 °C, then add 4 - 7 parts of vitamin E and 5 - 15 parts of β-carotene, and stir. S2: Add 1 - 5 parts of emulsifier to 20 - 35 parts of deionized water, heat to 45 - 55 °C and stir, then slowly add it to the mixed solution obtained in S1. S3: Add 5 - 8 parts of modified probiotic microcapsules and stir at 100 - 200 r / min for 8 - 12 min.

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