Optical imaging and electrophysiological recording window fixing device

Abstract

The invention discloses an optical imaging and electrophysiological recording window fixing device which comprises a window base, a window pressing flange ring, a fixing hollow screw and a top cover. According to the optical imaging and electrophysiological recording window fixing device, scalp cutting and windowing operations do not need to be repeatedly carried out, only one-time installation is needed, multiple experiments can be repeatedly carried out subsequently, and repeated injuries to experimental animals are reduced; normal intracranial pressure can be maintained, and the window is sealed in an anti-infection mode. The window base is firm in design and can be fixed to the surface of the skull through dental cement or bone cement. The window pressing flange ring can be replaced and selected to adapt to imaging windows of different animals and different sizes and depths. During an experiment interval, sealing is achieved through the top cover, effective sealing of the window can be guaranteed while operation is easy and convenient, and tissue hyperplasia or infection is reduced. An electrophysiological positioning device is compatible, and electrode implantation is facilitated.

Description

Technical Field

[0001] The invention belongs to the field of biotechnology, and in particular relates to an optical imaging and electrophysiological recording window fixing device. Background Art

[0002] Endogenous optical imaging technology uses a highly sensitive CCD camera to image the optical signals of the cerebral cortex, and detects the changes in local oxygen supply caused by brain neuron activity through the reflection of light of a specific wavelength, thereby revealing brain activity. The results of optical imaging usually reflect the surface signals of the cerebral cortex. Researchers use this technology to obtain cortical functional maps of the brain area of ​​interest. Furthermore, it can be combined with electrophysiological technology to detect neuronal activity at different cortical depths at specific functional locations, thereby revealing the function of brain areas in a more systematic way.

[0003] In many studies, we need to repeatedly image and record electrophysiological data from the same area of ​​the brain of experimental animals. This requires a reliable and stable device that can meet the experimental requirements and can be continuously maintained to prevent infection and tissue proliferation for repeated experiments.

[0004] In previous repeated experimental plans, researchers used flange rings to connect glass slides, pressed them on the surface of the brain, and used several small wooden strips to temporarily fix them on the skull with glue for imaging experiments. At the end of the experiment, they used artificial meninges to press the window, put it back into the skull, sealed the window with bone cement, and sutured the wound. Secondary or multiple experiments require scalp cutting and window surgery at the same location. This plan inevitably causes repeated surgical injuries to animals. At the same time, since the surgical operation increases the duration of the anesthesia experiment, it is not conducive to experimental data collection, and it is also not conducive to animal recovery.

[0005] For example, the invention application with publication number CN113855303A discloses a cranial window for observing brain drug administration and an experimental animal fixation observation device. The cranial window for observing brain drug administration includes a main body and a light-transmitting plate. The main body is provided with a protruding portion. The end face of the protruding portion is provided with a light-transmitting hole and a first perforation. The circumferential surface of the protruding portion is provided with a second perforation. The first perforation and the second perforation are used to cooperate with the drug administration tube of the drug administration pump. The light-transmitting plate is matched on the protruding portion to cover the light-transmitting hole.

[0006] For another example, the utility model with authorization announcement number CN219166501U discloses a skull tissue sealing device and a craniotomy imaging device, wherein the skull tissue sealing device comprises an imaging glass slide and a sealing ring, wherein the optical axis of the imaging glass slide coincides with the central axis of the sealing ring, and one end of the sealing ring is sealed with the imaging glass slide to form a container, wherein a cavity for accommodating a hydrogel is formed inside the container, and an opening is formed at the other end of the sealing ring; and an adhesive layer for sealing and bonding with the skull is provided on the outer surface of the sealing ring. Summary of the invention

[0007] To solve these problems, we designed an imaging and electrophysiological window fixing device that can be permanently fixed to the animal's head and is convenient for disassembly and cleaning of the window. After experimental testing, the device can meet the requirements of imaging and electrophysiological experiments for windows, and is convenient for long-term maintenance and can be used for repeated experiments.

[0008] An optical imaging and electrophysiological recording window fixing device, comprising:

[0009] The window base is used for fitting and mounting on the surface of the skull, the window base has a mounting slot, the bottom of the inner side of the mounting slot has a step structure, and the inner side of the mounting slot above the step structure has an internal thread;

[0010] A window-pressing flange ring, comprising a pressing ring and a retaining ring extending downward from the inner ring of the bottom surface of the pressing ring. When in use, a glass slide is installed on the lower surface of the retaining ring and extends into the target window of the skull to be installed. The pressing ring overlaps the step structure. The glass slide used in the maintenance stage has no hole, while the glass slide used in electrophysiological testing has a lower electrode hole.

[0011] The fixed hollow screw is a ring with external threads. When in use, the fixed hollow screw cooperates with the internal threads of the window base to be installed in the installation slot hole, and presses and fixes the window pressing flange ring;

[0012] The top cover is fixedly mounted on the window base during the maintenance phase and is used to cover the mounting slot.

[0013] Preferably, the top surface outer circumference of the window base has a plurality of threaded fixing holes, the top cover is correspondingly provided with a first mounting hole, and the top cover and the window base are fixed by bolts passing through the first mounting holes and cooperating with the threaded fixing holes.

[0014] More preferably, the optical imaging and electrophysiological recording window fixing device further comprises a flange ring fixing part, the flange ring fixing part has a second mounting hole corresponding to the position of the threaded fixing hole on the window base, the structure of the flange ring fixing part avoids the mounting slot hole, and the flange ring fixing part has a fixing arm extending toward the mounting slot hole position, the fixing arm is provided with a threaded fixing through hole, and the window pressing flange ring is fixed by a fixing bolt matched with the threaded fixing through hole against the top surface of the pressing ring of the window pressing flange ring;

[0015] When in use, the fixing hollow screw is used to compress and fix the window pressing flange ring during the maintenance phase, and the flange ring fixing piece is used to compress and fix the window pressing flange ring during electrophysiological testing.

[0016] Further preferably, the optical imaging and electrophysiological recording window fixing device also includes an electrophysiological positioning device connecting piece, the electrophysiological positioning device connecting piece has a fixing area at the bottom and a supporting table at the top, the fixing area has a third mounting hole corresponding to the position of the remaining threaded fixing hole on the window base, and the structure of the electrophysiological positioning device connecting piece avoids the mounting slot hole.

[0017] When in use, during electrophysiological testing, the electrophysiological positioning device connector is fixed to the window base by a bolt passing through the third mounting hole, the electrode mounting arm of the electrophysiological recording device is supported on the support table, and the electrode extends into the mounting slot and passes through the lower electrode hole on the glass slide.

[0018] Further preferably, the window base is provided with four threaded fixing holes located at four corners, the flange ring fixing piece is provided with two second mounting holes, and the fixing area of ​​the electrophysiological positioning device connecting piece is provided with two third mounting holes.

[0019] Preferably, when in use, the window base is fitted on the skull surface using dental cement or bone cement, and the outer periphery of the window base is provided with grooves for increasing friction, wherein the grooves are arranged vertically at intervals or crisscrossed.

[0020] Preferably, the bottom surface of the window base has a lower edge extending outwardly along the mounting slot, and when in use, the lower edge extends into the target window of the skull to be mounted.

[0021] Preferably, a wrench action groove is provided on the top surface of the fixed hollow screw.

[0022] Preferably, a convex ring extending into the installation slot is convexly provided on the bottom surface of the top cover, and the outer diameter of the convex ring matches the installation slot.

[0023] The present invention further provides an electrophysiological recording method, wherein the optical imaging and electrophysiological recording window fixing device is installed on the surface window of the skull of an experimental animal.

[0024] During electrophysiological recording, the top cover is opened for testing; after the testing is completed, the top cover is used to close it.

[0025] When the optical imaging and electrophysiological recording window fixing device of the present invention is used, the window installation steps are as follows:

[0026] (1) The dura mater is exposed by opening a target window on the surface of the skull of the experimental animal, wherein the size of the window matches the size of the retaining ring at the bottom of the window pressing flange ring.

[0027] (2) Grind the surface near the window of the skull, fit the window base to the surface of the skull, and align the installation slot with the window position. To ensure alignment, when installing the window base, the window pressing flange ring can be placed into the window base, and the retaining ring at the bottom of the window pressing flange ring can be extended into the window to play a positioning role. Then use dental cement or bone cement to coat the periphery and bottom of the window base, wait for it to solidify, and then fix the window base to the skull.

[0028] (3) Clean the window, peel off the dura mater to expose the cortical pia mater, measure the depth with a tape measure, and then install a window-pressing flange ring with a glass slide (without holes) bonded to the bottom and an overall thickness adapted to the window depth into the window base. In order to improve the sealing effect, a silicone sealing ring can be provided between the bottom surface of the window-pressing flange ring and the step structure of the window base.

[0029] (4) Tighten the fixed hollow screw into the window base to tighten the window flange ring below. Observe the surface and periphery of the glass slide. If there is no liquid seepage, or try to drop physiological saline on the surface and the depth does not change, it means that the seal is good.

[0030] (5) After the test is completed, add a few drops of antibiotic injection solution to the sealed surgical window, then install the top cover to the top surface of the window base, and seal the surrounding gaps again with bone wax to complete the window installation steps.

[0031] When conducting optical imaging inspection, you only need to open the top cover to conduct observation and inspection.

[0032] When conducting electrophysiological recording tests, you need to open the top cover first, then unscrew the fixed hollow screw, replace the window-pressing flange ring with the same model but with a lower electrode hole left on the glass slide, and install the electrode for testing.

[0033] The optical imaging and electrophysiological recording window fixing device of the present invention does not require repeated scalp cutting and window opening surgery, only needs to be installed once, and multiple experiments can be repeated subsequently, reducing repeated damage to experimental animals; it can maintain normal intracranial pressure and seal the window to prevent infection.

[0034] The window base is designed to be firmly fixed to the skull surface by dental cement or bone cement. The window flange ring can be replaced to adapt to different animals, different sizes and depths of imaging windows. During the experiment interval, the top cover is used to achieve sealing, which is easy to operate and can ensure effective sealing of the window to reduce tissue hyperplasia or infection. Compatible with electrophysiological positioning devices to facilitate electrode implantation. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 It is a schematic diagram of the assembly structure of the optical imaging and electrophysiological recording window fixing device of the present invention during the maintenance stage.

[0036] Figure 2 This is a schematic diagram of the assembly structure from another perspective during the maintenance phase of the optical imaging and electrophysiological recording window fixing device of the present invention.

[0037] Figure 3 It is a cross-sectional view of the assembly structure of the optical imaging and electrophysiological recording window fixing device of the present invention during the maintenance stage.

[0038] Figure 4 It is a schematic diagram of the exploded structure of the assembly structure during the maintenance stage of the optical imaging and electrophysiological recording window fixing device of the present invention.

[0039] Figure 5 Schematic diagram of the assembly structure for electrophysiological recording detection.

[0040] Figure 6 It is a schematic diagram of the structure of the flange ring fixing.

[0041] Figure 7 This is a schematic diagram of the structure of the connector of the electrophysiological positioning device.

[0042] Figure 8 Schematic diagram of the assembly structure for optical imaging detection.

[0043] Fig. 9 This is a cross-sectional view of the assembly structure during optical imaging inspection.

[0044] Fig.10 It is a schematic diagram of the structure of a hollow screw fastener.

[0045] Reference numerals:

[0046] Window base 1, step structure 11, threaded fixing hole 12, groove 13, bottom edge 14,

[0047] Window pressure flange ring 2, pressure ring 21, retaining ring 22,

[0048] Fix the hollow screw 3, the wrench action groove 31,

[0049] Top cover 4, first mounting hole 41, convex ring 42,

[0050] Flange ring fixing member 5, second mounting hole 51, fixing arm 52, threaded fixing through hole 53,

[0051] Electrophysiological positioning device connector 6, fixing area 61, support table 62, third mounting hole 63,

[0052] Tighten the wrench 7. DETAILED DESCRIPTION

[0053] like Figures 1 to 4As shown, an optical imaging and electrophysiological recording window fixing device is an assembly diagram structure in the maintenance stage, including a window base 1, a window pressing flange ring 2, a fixing hollow screw 3 and a top cover 4.

[0054] The window base 1 is used to fit on the surface of the skull. The window base 1 is an annular structure as a whole, with a mounting slot in the middle. The bottom of the mounting slot has a step structure 11, and the part of the mounting slot above the step structure 11 has an internal thread.

[0055] When in use, the window base 1 is fitted on the surface of the skull using dental cement or bone cement, and the outer periphery of the window base 1 is provided with grooves 13 for increasing friction. A plurality of grooves 13 are arranged vertically at intervals. Dental cement or bone cement can be embedded in the grooves 13, so that the installation and fixation of the window base 1 is more firmly secured.

[0056] In one embodiment, Figure 3 In the cross-sectional view shown, the bottom surface of the window base 1 is a plane. In another embodiment, as Fig. 9 As shown, the bottom surface of the window base 1 has a lower edge 14 extending outwardly along the mounting slot. When in use, the lower edge 14 extends into the target window of the skull to be mounted.

[0057] The window pressing flange ring 2 includes a pressing ring 21 and a retaining ring 22 extending downward from the inner circle of the bottom surface of the pressing ring 21. When in use, a glass slide (not shown) is installed on the lower surface of the retaining ring 22 and extends into the target window of the skull to be installed. The pressing ring 21 overlaps the step structure 11. The glass slide used in the maintenance stage has no hole, while the glass slide used in electrophysiological testing has a lower electrode hole.

[0058] The window pressing flange ring 2 needs to take into account windows of different sizes and different depths on the skull, so the window pressing flange ring 2 can be designed with different diameters and different heights of the retaining ring 22. When in use, the window pressing flange ring 2 is fixed to the glass slide of the corresponding model with UV-curing glue. The glass slide is round and its diameter is equal to the outer diameter of the retaining ring 22. During the experiment, the selection is made according to the specific situation. The depth selection of the window pressing flange ring 2 ensures that a matching depth can be selected during the experiment, that is, the cortical surface fluctuation caused by the retaining ring 22 of the window pressing flange ring 2 extending too shallowly will not affect the optical imaging, and the retaining ring 22 of the window pressing flange ring 2 extending too deeply will not cause insufficient blood supply to the local cortex.

[0059] The fixed hollow screw 3 is a ring with external threads. When in use, the fixed hollow screw 3 is matched with the internal threads of the window base 1 and installed in the installation slot, and the window pressing flange ring 2 is pressed and fixed. The top surface of the fixed hollow screw 3 is provided with a wrench action groove 31. Fig.10As shown, a tightening spanner 7 for installing and removing the fixed hollow screw 3 is provided. The bottom of the tightening spanner 7 is inserted into two opposite spanner action grooves 31, and then the fixed hollow screw 3 can be fixed to the window base 1 or removed from the window base 1 by rotating. In the structure shown in the figure, the top surface of the fixed hollow screw 3 is provided with four spanner action grooves 31, and the four spanner action grooves 31 are evenly distributed in a circle, and two opposite spanner action grooves are a group.

[0060] The top cover 4 is a cover sheet structure, which is fixedly mounted to the window base 1 during the maintenance phase and is used to cover the installation slot.

[0061] The top surface of the window base 1 has a plurality of threaded fixing holes 12 on its periphery, and the top cover 4 is correspondingly provided with a first mounting hole 41. The top cover 4 and the window base 1 are fixed by bolts (bolts not shown in the figure) passing through the first mounting holes 41 and cooperating with the threaded fixing holes 12.

[0062] The bottom surface of the top cover 4 is provided with a convex ring 42 extending into the installation slot, and the outer diameter of the convex ring 42 matches the installation slot. The provision of the convex ring 42 can improve the sealing performance.

[0063] like Figures 5-6 As shown, the optical imaging and electrophysiological recording window fixing device of the present invention further includes a flange ring fixing member 5, which has a second mounting hole 51 corresponding to the position of the threaded fixing hole 12 on the upper part of the window base 1, and the flange ring fixing member 5 is fixed to the window base 1 by a fixing bolt (not shown) passing through the second mounting hole 51 and cooperating with the threaded fixing hole 12. The structure of the flange ring fixing member 5 avoids the mounting slot hole of the window base 1, and the flange ring fixing member 5 is roughly a semicircular ring structure.

[0064] The flange ring fixing part 5 has a fixing arm 52 extending toward the mounting slot, and a threaded fixing through hole 53 is provided on the fixing arm 52. The window pressing flange ring 2 is fixed by a fixing bolt (not shown) matched with the threaded fixing through hole 53 against the top surface of the pressing ring 21 of the window pressing flange ring 2.

[0065] During use, the fixed hollow screw 3 is used in conjunction with the fixing bolt to compress and fix the window-pressing flange ring 2 during the maintenance phase, and the flange ring fixing piece 5 is used to compress and fix the window-pressing flange ring 2 during electrophysiological testing. During electrophysiological testing, we need to select multiple recording sites according to the purpose of the experiment in combination with the functional map obtained by optical imaging. Therefore, the position of the electrode needs to be adjusted in one experiment. If the fixed hollow screw 3 is still used to compress and fix the window-pressing flange ring 2, then each time the position is adjusted, the fixed hollow screw 3 needs to be loosened first, and then the fixed hollow screw 3 needs to be tightened after the adjustment is completed, which is very troublesome to operate and use.

[0066] Therefore, the present invention uses the flange ring fixing part 5 in conjunction with the fixing bolts to temporarily fix the window pressure flange ring 2 during electrophysiological testing. When adjusting, it is only necessary to loosen the fixing bolts to adjust the angle of the window pressure flange ring 2, thereby adjusting the electrode position. After adjustment, re-tighten the fixing bolts to fix the window pressure flange ring 2. The entire adjustment process is relatively simple and convenient.

[0067] like Figure 5 and Figure 7 As shown, the optical imaging and electrophysiological recording window fixing device of the present invention also includes an electrophysiological positioning device connector 6, which has a fixing area 61 at the bottom and a supporting table 62 at the top, and the fixing area 61 has a third mounting hole 63 corresponding to the position of the remaining threaded fixing hole 12 on the window base 1, and the electrophysiological positioning device connector 6 is fixed to the window base 1 by a fixing bolt (not shown) passing through the third mounting hole 63 and cooperating with the threaded fixing hole 12. The structure of the electrophysiological positioning device connector 6 avoids the mounting slot hole.

[0068] When in use, during electrophysiological testing, the electrophysiological positioning device connector 6 is fixed to the window base 1 by a bolt passing through the third mounting hole 63, and the hydraulic micro-push device of the electrophysiological recording electrode is fixed to the supporting table 62 by screws. The electrode extends into the mounting slot and passes through the lower electrode hole on the glass slide. This fixed setting can effectively ensure that the electrode implantation trajectory is perpendicular to the cortical mark surface.

[0069] In a preferred embodiment, Figure 7 As shown, the window base 1 is provided with four threaded fixing holes 12 at four corners, the flange ring fixing member 5 is provided with two second mounting holes 51, and the fixing area 61 of the electrophysiological positioning device connecting member 6 is provided with two third mounting holes 63. The flange ring fixing member 5 and the electrophysiological positioning device connecting member 6 are fixed to the window base 1 using fixing bolts respectively.

[0070] When the optical imaging and electrophysiological recording window fixing device of the present invention is used, the window installation steps are as follows:

[0071] (1) The dura mater is exposed by opening a target window on the surface of the skull of the experimental animal, wherein the size of the window matches the size of the retaining ring 22 at the bottom of the window pressing flange ring 2.

[0072] (2) Grind the surface near the window of the skull, fit the window base 1 to the surface of the skull, and align the installation slot with the window position. To ensure alignment, when installing the window base 1, the window pressing flange ring 2 can be placed into the window base 1, and the retaining ring 22 at the bottom of the window pressing flange ring 2 can be extended into the window to play a positioning role. Then use dental cement or bone cement to apply it on the periphery and bottom of the window base 1, wait for it to solidify, and then fix the window base 1 to the skull.

[0073] (3) Clean the window, peel off the dura mater to expose the cortical pia mater, measure the depth with a tape measure, and then install the window pressing flange ring 2 with a glass slide (without holes) bonded to the bottom and the overall thickness adapted to the window depth into the window base 1. In order to improve the sealing effect, a silicone sealing ring can be provided between the bottom surface of the pressing ring 21 of the window pressing flange ring 2 and the step structure 11 of the window base 1.

[0074] (4) Tighten the fixed hollow screw 3 into the window base 1 to tighten the window-pressing flange ring 2 below. Observe the surface and periphery of the glass slide. If there is no liquid seepage, or if a drop of saline solution is dropped onto the surface and the depth does not change, it indicates that the seal is good.

[0075] (5) After the test is completed, the top cover 4 is installed on the top surface of the window base 1, and the gap is sealed again with bone wax to complete the window installation steps.

[0076] When performing optical imaging detection, you only need to open the top cover 4 to conduct observation and detection. After the experiment, before installing the top cover 4, add a small amount of antibiotic injection to the installation slot to prevent infection. Then install the top cover 4, and use bone wax to further seal the joint between the top cover 4 and the window base 1 so that you can conduct experiments again later.

[0077] When conducting electrophysiological recording detection, it is necessary to first open the top cover 4, then unscrew the fixed hollow screw 3, and replace the window pressing flange ring 2 with the same model but with a lower electrode hole on the glass slide. Then, the flange ring fixing part 5 and the electrophysiological positioning device connecting part 6 are respectively fixed to the window base 1 using fixing bolts. Then, the fixing bolts that cooperate with the threaded fixing through hole 53 are used to press against the top surface of the pressing ring 21 of the window pressing flange ring 2 to fix the window pressing flange ring 2. The electrode mounting arm of the electrophysiological recording device is supported on the supporting table 62, and the electrode extends into the mounting slot and passes through the lower electrode hole on the glass slide. When the electrode position needs to be adjusted, loosen the fixing bolts used to tighten and fix the window pressing flange ring 2, and then adjust the angle of the window pressing flange ring 2 to adjust the electrode position. After adjustment, re-tighten the fixing bolts to fix the window pressing flange ring 2. After the test is completed, re-disassemble the flange ring fixing part 5 and the electrophysiological positioning device connecting part 6, and then use the fixed hollow screw 3 to re-tighten and fix the window pressing flange ring 2. Before installing the top cover 4, a small amount of antibiotic injection is first added to the installation slot to prevent infection. Then the top cover 4 is installed, and bone wax is used to further seal the joint between the top cover 4 and the window base 1 so that the experiment can be carried out again later.

Claims

1. An optical imaging and electrophysiological recording window fixing device, characterized in that: include: The window base is used for fitting and mounting on the surface of the skull, the window base has a mounting slot, the bottom of the inner side of the mounting slot has a step structure, and the inner side of the mounting slot above the step structure has an internal thread; The window-pressing flange ring includes a pressing ring and a retaining ring extending downward from the inner circle of the bottom surface of the pressing ring. When in use, a glass slide fixed with ultraviolet light curing glue is installed on the lower surface of the retaining ring. The retaining ring with the glass slide extends into the target window of the skull to be installed, and the pressing ring overlaps the step structure. The glass slide used in the maintenance stage has no holes, while the glass slide used in electrophysiological testing has a lower electrode hole. The fixed hollow screw is a ring with external threads. When in use, the fixed hollow screw cooperates with the internal threads of the window base to be installed in the installation slot hole, and presses and fixes the window pressing flange ring; The top cover is fixedly mounted on the window base during the maintenance phase and is used to cover the mounting slot.

2. The optical imaging and electrophysiological recording window fixing device according to claim 1, characterized in that: The top surface outer circumference of the window base is provided with a plurality of threaded fixing holes, and the top cover is correspondingly provided with a first mounting hole, and the top cover and the window base are fixed by bolts passing through the first mounting holes and cooperating with the threaded fixing holes.

3. The optical imaging and electrophysiological recording window fixing device according to claim 2, characterized in that: It also includes a flange ring fixing piece, the flange ring fixing piece has a second mounting hole corresponding to the position of the threaded fixing hole on the window base, the structure of the flange ring fixing piece avoids the mounting slot hole, and the flange ring fixing piece has a fixing arm extending toward the mounting slot hole position, the fixing arm is provided with a threaded fixing through hole, and the window pressing flange ring is fixed by a fixing bolt matched with the threaded fixing through hole against the top surface of the pressing ring of the window pressing flange ring; When in use, the fixing hollow screw is used to compress and fix the window pressing flange ring during the maintenance phase, and the flange ring fixing piece is used to compress and fix the window pressing flange ring during electrophysiological testing.

4. The optical imaging and electrophysiological recording window fixing device according to claim 3, characterized in that: It also includes an electrophysiological positioning device connector, which has a fixing area at the bottom and a supporting table at the top, and the fixing area has a third mounting hole corresponding to the position of the remaining threaded fixing hole on the window base, and the structure of the electrophysiological positioning device connector avoids the mounting slot hole. When in use, during electrophysiological testing, the electrophysiological positioning device connector is fixed to the window base by a bolt passing through the third mounting hole, the electrode propulsion device mounting arm of the electrophysiological recording device is supported on the support table, and the electrode extends into the mounting slot and passes through the lower electrode hole on the glass slide.

5. The optical imaging and electrophysiological recording window fixing device according to claim 4, characterized in that: The window base is provided with four threaded fixing holes located at four corners, the flange ring fixing piece is provided with two second mounting holes, and the fixing area of ​​the electrophysiological positioning device connecting piece is provided with two third mounting holes.

6. The optical imaging and electrophysiological recording window fixing device according to claim 1, characterized in that: When in use, the window base is fitted on the surface of the skull using dental cement or bone cement, and a groove for increasing friction is arranged on the periphery of the window base.

7. The optical imaging and electrophysiological recording window fixing device according to claim 1, characterized in that: The bottom surface of the window base has a lower edge extending outwardly along the installation slot. When in use, a circular skull window is opened with an area slightly larger than the lower edge of the base, and the lower edge is extended into the target window of the skull to be installed.

8. The optical imaging and electrophysiological recording window fixing device according to claim 1, characterized in that: The top surface of the fixed hollow screw is provided with a wrench action groove.

9. The optical imaging and electrophysiological recording window fixing device according to claim 1, characterized in that: The bottom surface of the top cover is convexly provided with a convex ring extending into the installation slot hole, and the outer diameter of the convex ring matches the installation slot hole.

10. An electrophysiological recording method, characterized in that: The optical imaging and electrophysiological recording window fixing device according to any one of claims 1 to 9 is mounted on the surface window of the skull of an experimental animal. During electrophysiological recording, the top cover is opened for testing; after the testing is completed, the top cover is used to close it.

Images