Medical polyglutamic acid functional dressing capable of whitening skin and fading spots and preparation method of medical polyglutamic acid functional dressing

Abstract

The invention relates to a medical polyglutamic acid functional dressing for whitening and fading spots and a preparation method thereof, and belongs to the technical field of dressings. The medical polyglutamic acid functional dressing provided by the invention comprises the following components in parts by weight: 0.03-0.07 part of sodium polyglutamate, 0.01-0.05 part of sodium hyaluronate, 0.01-0.09 part of benzyl alcohol and 99.74-100 parts of purified water, the metabolism of skin cells is stimulated by means of sodium polyglutamate, the activity of tyrosinase is inhibited, so that the generation of melanin is reduced, and in cooperation with components such as sodium hyaluronate and the like, the medical polyglutamic acid functional dressing has the effects of promoting the metabolism of skin cells and reducing the generation of melanin. Along with the increase of the service cycle, skin cells are full, skin pigmentation is reduced, spots are gradually faded, finally, the skin is compact, the skin color is uniform and glossy, and the whitening and spot fading effects are effectively achieved.

Description

Technical Field

[0001] The invention belongs to the technical field of dressings and relates to a medical polyglutamic acid functional dressing for whitening and lightening spots and a preparation method thereof. Background Art

[0002] In today's fast-paced world, due to various reasons from the external environment and internal factors, skin problems such as spots and dullness are common, and the demand for skin care products in the market continues to rise. In daily life, due to frequent exposure to strong ultraviolet rays, the skin will accelerate the production of a large amount of melanin in order to protect itself. As we age, the skin's metabolic capacity gradually weakens, and the ability to decompose and excrete melanin also decreases. In addition, staying up late, stress, irregular work and rest, improper skin care, etc. will also seriously affect the skin's metabolic function. After the metabolic capacity is weakened, the melanin metabolism capacity is blocked, and freckles, sun spots, and dark spots are formed in the epidermis of the skin. At present, whitening and spot-removing products are mainly divided into oral and topical. Oral administration is slow to take effect and long-term consumption is likely to increase kidney pressure. Topical application has become the first choice. However, some whitening and spot-removing products on the market may contain irritating ingredients, which are easy to increase the burden on the skin, or although ingredients with whitening and spot-removing effects are added, they cannot penetrate deep into the skin and have low absorption and utilization rates. Summary of the invention

[0003] The purpose of the present invention is to provide a medical polyglutamic acid functional dressing for whitening and lightening spots and a preparation method thereof, which uses sodium polyglutamate to stimulate the metabolism of skin cells and inhibit the activity of tyrosinase, thereby reducing the production of melanin, and cooperates with components such as sodium hyaluronate. As the use cycle increases, skin cells are filled and plump, skin pigmentation is reduced, and spots gradually fade, ultimately making the skin firm, the skin tone even and shiny, and effectively exerting the effects of whitening and lightening spots.

[0004] The purpose of the present invention can be achieved by the following technical solutions:

[0005] The invention discloses a medical polyglutamic acid functional dressing for whitening and lightening spots. The medical polyglutamic acid functional dressing comprises the following components in parts by weight: 0.03-0.07 parts of sodium polyglutamate, 0.01-0.05 parts of sodium hyaluronate, 0.01-0.09 parts of benzyl alcohol and 99.74-100 parts of purified water.

[0006] Furthermore, the preparation steps of the sodium polyglutamate are:

[0007] (1) Inoculate 0.9-1.1×10 Bacillus per liter of culture medium. 7 CFU ratio was inoculated into the fermentation medium, and cultured at 30-34°C for 42-54h, and during the fermentation process, 0.5L / min of oxygen was passed and the stirring speed was 4-10rmp, and the fermentation liquid was obtained after the fermentation was completed;

[0008] (2) centrifuging the fermentation liquid to remove the bacterial cells, and re-dissolving the naturally dried fermentation product in a blueberry extract, ultrasonicating, and stirring to obtain a composite fermentation product;

[0009] (3) The composite fermentation product is subjected to vacuum low-temperature freeze-drying to obtain sodium polyglutamate.

[0010] Furthermore, the raw materials of the fermentation medium in step (2) include 48-56 g / L sodium glutamate, 4.5-5.5 g / L yeast extract, 15.2-16 g / L trisodium citrate, 80-100 g / L glucose, 0.8-1.2 g / L potassium dihydrogen phosphate, 0.3-0.7 g / L magnesium sulfate, 5-15 g / L blueberry extract and the remainder purified water.

[0011] Furthermore, the pH value of the fermentation medium is 6-7, and after preparation, it needs to be sterilized in a 120° C. autoclave for 15-25 minutes.

[0012] Furthermore, the raw materials of the blueberry extract include 64-75.8wt% blueberry liquid, 1-3.2wt% vitamin C, 2.6-4wt% nicotinamide solution and the balance purified water by mass percentage; the nicotinamide solution consists of 3-8wt% nicotinamide and the balance purified water.

[0013] Furthermore, the preparation steps of the blueberry juice are:

[0014] Fresh blueberries are washed and impurities are removed, and the juice is squeezed. The juice and an ethanol solution with a mass concentration of 65-75wt% are mixed in a volume ratio of 1:4-6, and the mixture is stirred at a speed of 150-250rmp for 10-20min to be evenly mixed, and the mixture is allowed to stand for 1.2-1.8d. The centrifugal speed is set to 6000-9000rmp, and the mixture is centrifuged for 20-30min. The supernatant is taken, and the mixture is concentrated by rotary evaporation at 60°C to 1 / 4 of the original volume to obtain blueberry juice.

[0015] Furthermore, in step (2), the volume ratio of the fermentation product to the blueberry extract is 1:3-5; the rotation speed and time of the centrifugation step are 8000-12000 rpm and 15-25 min, respectively; the frequency, power and time of the ultrasound step are 30-40 kHz, 500-600 W and 10-20 min, respectively; the rotation speed and time of the stirring step are 100-200 rpm and 6-8 min, respectively.

[0016] Furthermore, the vacuum low-temperature freeze-drying in step (3) requires that the composite fermentation product be placed in a -80°C ultra-low temperature refrigerator for precooling for 8-12 hours, and finally freeze-dried at an air pressure of 1 Pa for 12-24 hours.

[0017] Furthermore, the preparation method of the medical polyglutamic acid functional dressing is:

[0018] Sequentially add purified water, sodium polyglutamate and sodium hyaluronate into the batching tank in proportion, set the speed of the batching tank to 20-30 rpm, stir for 4-6 minutes, heat to 80-90°C, keep warm for 15-25 minutes, keep the speed unchanged and stir while cooling to 35-45°C, add benzyl alcohol, continue stirring for 8-12 minutes, let stand, and sterilize for 1 hour to obtain a medical polyglutamic acid functional dressing.

[0019] Beneficial effects of the present invention:

[0020] (1) The sodium polyglutamate of the present invention accelerates the metabolism of cells by stimulating specific signal pathways in skin cells, so that the melanin accumulated in the cells is excreted from the body as the cells metabolize, thereby reducing melanin deposition; and sodium hyaluronate forms a moist protective film on the skin surface with its strong water absorption, which not only prevents water loss and makes skin cells full and plump, but also makes the skin firm, the skin tone even and shiny, and cooperates with sodium polyglutamate to achieve the effect of whitening and lightening spots.

[0021] (2) The present invention adds blueberry extract when preparing sodium polyglutamate, and blueberry extract is rich in multiple active ingredients. The antioxidant ingredients such as anthocyanins can effectively remove free radicals, reduce the stimulation of oxidative stress on melanin production, and inhibit its activity by interacting with tyrosinase, thereby reducing the production of melanin. In addition, the active ingredients in blueberry extract can also provide additional energy support for skin cells; vitamin C, as a powerful antioxidant, can effectively inhibit the activity of tyrosinase, thereby reducing the production of melanin; niacinamide can prevent melanin from transferring to epidermal cells and reduce pigment deposition; in addition, in the preparation process of sodium polyglutamate, blueberry extract is repeatedly added during the fermentation step and the re-dissolution treatment, which further improves the adhesion of the blueberry extract. With the inhibition of melanin production and the discharge of pigments, skin pigment deposition is reduced, and spots gradually fade, thereby exerting a whitening and spot-lightening effect. DETAILED DESCRIPTION

[0022] In order to further illustrate the technical means and effects adopted by the present invention to achieve the predetermined invention purpose, the specific implementation methods, structures, features and effects of the present invention are described in detail below in combination with the embodiments.

[0023] Example 1

[0024] A medical polyglutamic acid functional dressing for whitening and lightening spots. The medical polyglutamic acid functional dressing of this embodiment comprises the following components by weight: 0.03 parts of sodium polyglutamate, 0.01 parts of sodium hyaluronate, 0.01 parts of benzyl alcohol and 99.74 parts of purified water.

[0025] The preparation steps of the sodium polyglutamate of the present embodiment are:

[0026] (1) The Bacillus was inoculated at a rate of 0.9 × 10 7 CFU ratio was inoculated into the fermentation medium, and cultured at 30°C for 42-54h, and during the fermentation process, 0.5L / min of oxygen was passed and the stirring speed was 4rmp, and the fermentation liquid was obtained after the fermentation was completed;

[0027] (2) centrifuging the fermentation liquid to remove the bacterial cells, and re-dissolving the naturally dried fermentation product in a blueberry extract, ultrasonicating, and stirring to obtain a composite fermentation product;

[0028] (3) The composite fermentation product is subjected to vacuum low-temperature freeze-drying to obtain sodium polyglutamate.

[0029] The raw materials of the fermentation medium of this embodiment in step (2) include 48g / L sodium glutamate, 4.5g / L yeast extract, 15.2g / L trisodium citrate, 80g / L glucose, 0.8g / L potassium dihydrogen phosphate, 0.3g / L magnesium sulfate, 5g / L blueberry extract and the balance purified water.

[0030] The pH value of the fermentation medium in this embodiment is 6, and after preparation, it needs to be sterilized in a pressure cooker at 120° C. for 15 minutes.

[0031] The raw materials of the blueberry extract of this embodiment include 64wt% blueberry liquid, 1wt% vitamin C, 2.6wt% niacinamide solution and the balance purified water by mass percentage; the niacinamide solution of this embodiment consists of 3wt% niacinamide and the balance purified water.

[0032] The preparation steps of the blueberry juice of this embodiment are:

[0033] After washing and removing impurities from fresh blueberries, the juice was squeezed and mixed with an ethanol solution with a mass concentration of 65wt% in a volume ratio of 1:4, stirred at a speed of 150rmp for 10min to mix evenly, allowed to stand for 1.2d, the centrifugal speed was set to 6000rmp, centrifuged for 20min, the supernatant was taken, and concentrated by rotary evaporation at 60°C to 1 / 4 of the original volume to obtain blueberry juice.

[0034] In step (2), the volume ratio of the fermentation product and the blueberry extract of this embodiment is 1:3; the speed and time of the centrifugation step of this embodiment are 8000 rpm and 15 min respectively; the frequency, power and time of the ultrasonic step of this embodiment are 30 kHz, 500 W and 10 min respectively; the speed and time of the stirring step of this embodiment are 100 rpm and 6 min respectively.

[0035] In step (3), the vacuum low-temperature freeze-drying of this embodiment requires that the composite fermentation product is first placed in a -80°C ultra-low temperature refrigerator for precooling for 8 hours, and finally freeze-dried at an air pressure of 1 Pa for 12 hours.

[0036] The preparation method of the medical polyglutamic acid functional dressing of this embodiment is:

[0037] Purified water, sodium polyglutamate and sodium hyaluronate were added into the mixing tank in proportion, the speed of the mixing tank was set to 20 rpm, the temperature was raised to 80°C after stirring for 4 minutes, and the temperature was kept for 15 minutes. The temperature was lowered to 35°C while stirring while keeping the speed unchanged, benzyl alcohol was added, stirring was continued for 8 minutes, the mixture was allowed to stand, and sterilized for 1 hour to obtain a medical polyglutamic acid functional dressing.

[0038] Example 2

[0039] A medical polyglutamic acid functional dressing for whitening and lightening spots. The medical polyglutamic acid functional dressing of this embodiment comprises the following components in parts by weight: 0.07 parts of sodium polyglutamate, 0.05 parts of sodium hyaluronate, 0.09 parts of benzyl alcohol and 100 parts of purified water.

[0040] The preparation steps of the sodium polyglutamate of the present embodiment are:

[0041] (1) The inoculation rate of Bacillus was 1.1×10 7 The ratio of CFU was inoculated into the fermentation medium, and the culture was carried out at 34°C for 54 hours. During the fermentation process, 0.5 L / min of oxygen was passed and the stirring speed was 10 rpm. The fermentation liquid was obtained after the fermentation was completed.

[0042] (2) centrifuging the fermentation liquid to remove the bacterial cells, and re-dissolving the naturally dried fermentation product in a blueberry extract, ultrasonicating, and stirring to obtain a composite fermentation product;

[0043] (3) The composite fermentation product is subjected to vacuum low-temperature freeze-drying to obtain sodium polyglutamate.

[0044] The raw materials of the fermentation medium of this embodiment in step (2) include 56 g / L sodium glutamate, 5.5 g / L yeast extract, 16 g / L trisodium citrate, 100 g / L glucose, 1.2 g / L potassium dihydrogen phosphate, 0.7 g / L magnesium sulfate, 15 g / L blueberry extract and the remainder purified water.

[0045] The pH value of the fermentation medium in this embodiment is 7, and after preparation, it needs to be sterilized in a pressure cooker at 120° C. for 25 minutes.

[0046] The raw materials of the blueberry extract of this embodiment include 75.8wt% blueberry liquid, 3.2wt% vitamin C, 4wt% niacinamide solution and the balance purified water by mass percentage; the niacinamide solution of this embodiment consists of 8wt% niacinamide and the balance purified water.

[0047] The preparation steps of the blueberry juice of this embodiment are:

[0048] After washing and removing impurities from fresh blueberries, the juice was squeezed and mixed with an ethanol solution with a mass concentration of 75wt% in a volume ratio of 1:6, stirred at a speed of 250rmp for 20min to mix evenly, allowed to stand for 1.8d, the centrifugal speed was set to 9000rmp, centrifuged for 30min, the supernatant was taken, and concentrated by rotary evaporation at 60°C to 1 / 4 of the original volume to obtain blueberry juice.

[0049] In step (2), the volume ratio of the fermentation product of this embodiment to the blueberry extract is 1:5; the rotation speed and time of the centrifugation step of this embodiment are 12000 rpm and 25 min respectively; the frequency, power and time of the ultrasonic step of this embodiment are 40 kHz, 600 W and 20 min respectively; the rotation speed and time of the stirring step of this embodiment are 200 rpm and 8 min respectively.

[0050] In step (3), the vacuum low-temperature freeze-drying of this embodiment requires that the composite fermentation product is first placed in a -80°C ultra-low temperature refrigerator for precooling for 12 hours, and finally freeze-dried at an air pressure of 1 Pa for 24 hours.

[0051] The preparation method of the medical polyglutamic acid functional dressing of this embodiment is:

[0052] Add purified water, sodium polyglutamate and sodium hyaluronate into the mixing tank in proportion, set the speed of the mixing tank to 30 rpm, stir for 6 minutes, heat to 90°C, keep warm for 25 minutes, keep the speed unchanged and stir to cool to 45°C, add benzyl alcohol, continue stirring for 12 minutes, let stand, and sterilize for 1 hour to obtain a medical polyglutamic acid functional dressing.

[0053] Example 3

[0054] A medical polyglutamic acid functional dressing for whitening and lightening spots. The medical polyglutamic acid functional dressing of this embodiment comprises the following components in parts by weight: 0.05 parts of sodium polyglutamate, 0.03 parts of sodium hyaluronate, 0.05 parts of benzyl alcohol and 99.87 parts of purified water.

[0055] The preparation steps of the sodium polyglutamate of the present embodiment are:

[0056] (1) Inoculate Bacillus at a rate of 1×10 7The ratio of CFU was inoculated into the fermentation medium, and the culture was carried out at 32°C for 46 hours. During the fermentation process, 0.5 L / min of oxygen was passed and the stirring speed was 7 rpm. The fermentation liquid was obtained after the fermentation was completed.

[0057] (2) centrifuging the fermentation liquid to remove the bacterial cells, and re-dissolving the naturally dried fermentation product in a blueberry extract, ultrasonicating, and stirring to obtain a composite fermentation product;

[0058] (3) The composite fermentation product is subjected to vacuum low-temperature freeze-drying to obtain sodium polyglutamate.

[0059] The raw materials of the fermentation medium of this embodiment in step (2) include 52 g / L sodium glutamate, 5 g / L yeast extract, 15.6 g / L trisodium citrate, 90 g / L glucose, 1 g / L potassium dihydrogen phosphate, 0.5 g / L magnesium sulfate, 10 g / L blueberry extract and the remainder purified water.

[0060] The pH value of the fermentation medium in this embodiment is 6.5, and after preparation, it needs to be sterilized in a pressure cooker at 120° C. for 20 minutes.

[0061] The raw materials of the blueberry extract of this embodiment include 69.9wt% blueberry liquid, 2.1wt% vitamin C, 3.3wt% niacinamide solution and the balance purified water by mass percentage; the niacinamide solution of this embodiment consists of 5.5wt% niacinamide and the balance purified water.

[0062] The preparation steps of the blueberry juice of this embodiment are:

[0063] After washing and removing impurities from fresh blueberries, the juice was squeezed and mixed with an ethanol solution with a mass concentration of 70wt% in a volume ratio of 1:5, stirred at a speed of 200rpm for 15min to mix evenly, allowed to stand for 1.5d, the centrifugal speed was set to 7500rpm, centrifuged for 25min, the supernatant was taken, and concentrated by rotary evaporation at 60°C to 1 / 4 of the original volume to obtain blueberry juice.

[0064] In step (2), the volume ratio of the fermentation product and the blueberry extract of this embodiment is 1:4; the rotation speed and time of the centrifugation step of this embodiment are 10000 rpm and 20 min respectively; the frequency, power and time of the ultrasonic step of this embodiment are 35 kHz, 550 W and 15 min respectively; the rotation speed and time of the stirring step of this embodiment are 150 rpm and 7 min respectively.

[0065] In step (3), the vacuum low-temperature freeze-drying of this embodiment requires that the composite fermentation product is first placed in a -80°C ultra-low temperature refrigerator for precooling for 10 hours, and finally freeze-dried at an air pressure of 1 Pa for 18 hours.

[0066] The preparation method of the medical polyglutamic acid functional dressing of this embodiment is:

[0067] Add purified water, sodium polyglutamate and sodium hyaluronate into the mixing tank in proportion, set the speed of the mixing tank to 25 rpm, stir for 5 minutes and then heat to 85°C, keep warm for 20 minutes, keep the speed unchanged and stir while cooling to 40°C, add benzyl alcohol, continue stirring for 10 minutes, let stand, and sterilize for 1 hour to obtain a medical polyglutamic acid functional dressing.

[0068] Comparative Example 1

[0069] On the basis of Example 3, the blueberry extract in the fermentation medium was removed and replaced with an equal weight of purified water. Other conditions were the same as those in Example 3.

[0070] Comparative Example 2

[0071] On the basis of Example 3, the nicotinamide solution in the blueberry extract was removed and replaced with an equal weight of purified water, and other conditions were consistent with Example 3.

[0072] Comparative Example 3

[0073] On the basis of Example 3, vitamin C in the blueberry extract was removed and replaced with an equal weight of purified water. Other conditions were the same as in Example 3.

[0074] Comparative Example 4

[0075] On the basis of Example 3, keeping other conditions consistent with Example 3, the preparation steps of sodium polyglutamate are changed to:

[0076] (1) Inoculate Bacillus at a rate of 1×10 7 The ratio of CFU was inoculated into the fermentation medium, and the culture was carried out at 32°C for 46 hours. During the fermentation process, 0.5 L / min of oxygen was passed and the stirring speed was 7 rpm. The fermentation liquid was obtained after the fermentation was completed.

[0077] (2) The fermentation liquid is centrifuged to remove the bacterial cells, and then subjected to vacuum low-temperature freeze-drying to obtain sodium polyglutamate.

[0078] 1. Skin patch test

[0079] Thirty volunteers with healthy skin were selected, half male and half female, aged between 20 and 50 years old. All volunteers had no history of skin disease, no known allergy to the test materials, and had not used any drugs or cosmetics that might affect skin reactions within at least 2 weeks before the test.

[0080] The dressing prepared in Examples 1-3 was used as a sample, 0.02-0.03 g of the sample was placed in a spot tester, and applied to the flexor side of the subject's forearm with a low-allergenic tape. The patch was gently pressed with the palm of the hand to evenly apply it to the skin. After 24 hours, the spot tester was removed, and the skin reactions were observed 0.5 h, 6 h, 12 h, and 24 h after removal. The results were recorded according to the skin reaction grading standards in the "Hygiene Standards for Cosmetics" and the "Safety Technical Standards for Cosmetics".

[0081] Among them, the scoring grading is divided into 0-4 levels, 0 is a negative reaction; 1 is a suspicious reaction, with only faint erythema; 2 is a weak positive reaction, with erythema, infiltration, edema, and papules; 3 is a strong positive reaction, with erythema, infiltration, edema, papules, and the reaction may exceed the test area; 4 is a very strong positive reaction, accompanied by obvious erythema, severe infiltration, edema, confluent herpes, and the reaction exceeds the test area; the test results are shown in Table 1.

[0082] Table 1 Skin patch test results

[0083]

[0084] As can be seen from Table 1, the medical polyglutamic acid functional dressing prepared by the present invention has mild ingredients, is safe, and has no adverse skin reactions when used.

[0085] 2. Whitening effect test

[0086] The medical polyglutamic acid functional dressings prepared in Examples 1-3 and Comparative Examples 1-4 were used as samples, and volunteers aged 20-50, half male and half female, were selected. All volunteers had no history of skin disease, no known allergies to the test materials, and had not used any drugs or cosmetics that might affect skin reactions within at least 2 weeks before the test.

[0087] Each group of samples corresponded to 10 volunteers (half male and half female), who applied 0.2 g of the samples on a 3 cm × 3 cm area of ​​facial skin every morning and evening for 4 weeks. The MPA580 multi-functional skin tester was used to test the ITA values ​​before use and after 4 weeks of use to evaluate the whitening effect of the samples. The average values ​​of each group of samples were taken and recorded as shown in Table 2.

[0088] Table 2

[0089] When not in use After 4 weeks of use Example 1 39 62 Example 2 40 61 Example 3 38 63 Comparative Example 1 39 51 Comparative Example 2 37 53 Comparative Example 3 40 57 Comparative Example 4 39 48

[0090] As shown in Table 2, the whitening effect of the medical polyglutamic acid functional dressing prepared in Examples 1-3 of the present invention is better than that of the products prepared in Comparative Examples 1-4, and has good whitening effect. However, Comparative Examples 1-3 removed the blueberry extract or replaced a certain component in the blueberry extract, and could not achieve the inhibitory and clearing effect of the melanin brought about by the synergy of the components in the embodiments, and the whitening effect was weakened; Comparative Example 4 removed the redissolution treatment of the fermentation product by the blueberry extract, which may cause the loss of components during the preparation process, and lacked the gain effect of the superposition treatment, so the whitening effect was weakened.

[0091] 3. Spot lightening effect test

[0092] The medical polyglutamic acid functional dressings prepared in Examples 1-3 and Comparative Examples 1-4 were used as samples, and female volunteers aged 25-45 were selected. All volunteers had no history of skin disease, no known allergies to the test materials, and had not used any drugs or cosmetics that might affect skin reactions within at least 2 weeks before the test.

[0093] Each group of samples corresponded to 10 volunteers, who applied 0.5 g of the samples on their facial skin every morning and evening for one month. The cheek area was tested using a VISA digital skin analyzer. During the test, smile lines, reflective areas, moles and hair were avoided as much as possible. The spot-lightening effect of the samples was evaluated by comparing the spots before use, after one month of use, and after one month of discontinuation. The spot reduction rate of each group and the spot rebound rate after one month of discontinuation were calculated, and the average values ​​were taken and recorded as shown in Table 3.

[0094] Table 3

[0095]

[0096] As shown in Table 3, the medical polyglutamic acid functional dressings prepared in Examples 1-3 of the present invention have better freckle effect than the products prepared in Comparative Examples 1-4, have good freckle effect, and do not rebound after discontinuation of use. However, any change or replacement of the components or steps in the preparation steps of sodium polyglutamate of the present invention will lead to a weakening of the synergistic effect, and affect the inhibition and removal of melanin, thereby leading to a weakening of the spot lightening effect, and there is also the possibility of spot rebound.

[0097] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any form. Although the present invention has been disclosed as a preferred embodiment as above, it is not used to limit the present invention. Any technical personnel in this field can make some changes or modify the technical contents disclosed above into equivalent embodiments without departing from the scope of the technical solution of the present invention. However, any brief modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention are still within the scope of the technical solution of the present invention.

Claims

1. A medical polyglutamic acid functional dressing for whitening and lightening spots, characterized in that: The medical polyglutamic acid functional dressing comprises the following components in parts by weight: 0.03-0.07 parts of sodium polyglutamate, 0.01-0.05 parts of sodium hyaluronate, 0.01-0.09 parts of benzyl alcohol and 99.74-100 parts of purified water.

2. The medical polyglutamic acid functional dressing for whitening and lightening spots according to claim 1, characterized in that: The preparation steps of described sodium polyglutamate are: (1) Inoculate 0.9-1.1×10 Bacillus per liter of culture medium. 7 CFU ratio was inoculated into the fermentation medium, and cultured at 30-34°C for 42-54h, and during the fermentation process, 0.5L / min of oxygen was passed and the stirring speed was 4-10rmp, and the fermentation liquid was obtained after the fermentation was completed; (2) centrifuging the fermentation liquid to remove the bacterial cells, and re-dissolving the naturally dried fermentation product in a blueberry extract, ultrasonicating, and stirring to obtain a composite fermentation product; (3) The composite fermentation product is subjected to vacuum low-temperature freeze-drying to obtain sodium polyglutamate.

3. The medical polyglutamic acid functional dressing for whitening and lightening spots according to claim 2, characterized in that: The raw materials of the fermentation medium in step (2) include 48-56 g / L sodium glutamate, 4.5-5.5 g / L yeast extract, 15.2-16 g / L trisodium citrate, 80-100 g / L glucose, 0.8-1.2 g / L potassium dihydrogen phosphate, 0.3-0.7 g / L magnesium sulfate, 5-15 g / L blueberry extract and the balance purified water.

4. The medical polyglutamic acid functional dressing for whitening and lightening spots according to claim 3, characterized in that: The pH value of the fermentation medium is 6-7, and after preparation, it needs to be sterilized in a 120° C. autoclave for 15-25 minutes.

5. The medical polyglutamic acid functional dressing for whitening and lightening spots according to claim 2 or 3, characterized in that: The raw materials of the blueberry extract include 64-75.8wt% blueberry liquid, 1-3.2wt% vitamin C, 2.6-4wt% nicotinamide solution and the balance purified water by mass percentage; the nicotinamide solution is composed of 3-8wt% nicotinamide and the balance purified water.

6. The medical polyglutamic acid functional dressing for whitening and lightening spots according to claim 5, characterized in that: The preparation steps of the blueberry liquid are: Fresh blueberries are washed and impurities are removed, and the juice is squeezed. The juice and an ethanol solution with a mass concentration of 65-75wt% are mixed in a volume ratio of 1:4-6, and the mixture is stirred at a speed of 150-250rmp for 10-20min to be evenly mixed, and the mixture is allowed to stand for 1.2-1.8d. The centrifugal speed is set to 6000-9000rmp, and the mixture is centrifuged for 20-30min. The supernatant is taken, and the mixture is concentrated by rotary evaporation at 60°C to 1 / 4 of the original volume to obtain blueberry juice.

7. The medical polyglutamic acid functional dressing for whitening and lightening spots according to claim 2, characterized in that: In step (2), the volume ratio of the fermentation product to the blueberry extract is 1:3-5; the rotation speed and time of the centrifugation step are 8000-12000 rpm and 15-25 min respectively; the frequency, power and time of the ultrasound step are 30-40 kHz, 500-600 W and 10-20 min respectively; the rotation speed and time of the stirring step are 100-200 rpm and 6-8 min respectively.

8. The medical polyglutamic acid functional dressing for whitening and lightening spots according to claim 2, characterized in that: The vacuum low-temperature freeze-drying in step (3) requires that the composite fermentation product be placed in a -80°C ultra-low temperature refrigerator for precooling for 8-12 hours, and finally freeze-dried at an air pressure of 1 Pa for 12-24 hours.

9. A method for preparing the medical polyglutamic acid functional dressing for whitening and lightening spots according to any one of claims 1 to 8, characterized in that: The preparation method is: Sequentially add purified water, sodium polyglutamate and sodium hyaluronate into the batching tank in proportion, set the speed of the batching tank to 20-30 rpm, stir for 4-6 minutes, heat to 80-90°C, keep warm for 15-25 minutes, keep the speed unchanged and stir while cooling to 35-45°C, add benzyl alcohol, continue stirring for 8-12 minutes, let stand, and sterilize for 1 hour to obtain a medical polyglutamic acid functional dressing.

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