Natural plant eyeliner containing eyelash growth ingredients and preparation method thereof

Abstract

The present invention relates to a natural plant-based eyeliner containing eyelash growth ingredients and a preparation method thereof, belonging to the technical field of biological products. The eyeliner of the present invention contains an Argania spinosa kernel extract and a Jojoba seed extract prepared through a specific fermentation and enzymatic hydrolysis process. When used in combination with other ingredients, the eyeliner promotes eyelash growth, enhances eyelash vitality, and reduces eyelash shedding. The Argania spinosa kernel extract and the Jojoba seed extract do not affect the long-lasting effect of the eyeliner. The combined use of the Argania spinosa kernel extract and the Jojoba seed extract has a good synergistic effect in promoting eyelash growth, significantly increasing eyelash length and density, protecting eyelashes and reducing damage to cosmetics, preventing eyelash shedding, maintaining eyelash density, and being safe and free of side effects.

Description

Technical Field

[0001] The invention belongs to the technical field of biological products, and particularly relates to a natural plant eyeliner containing eyelash growth ingredients and a preparation method thereof. Background Art

[0002] In today's beauty industry, liquid eyeliner has become a crucial eye makeup product, applied to the base of the lashes to enhance the eyes' mystique and allure. Traditional liquid eyeliner is primarily composed of a colorant, solvent, film-forming agent, and other additives. The colorant imparts its color, with classic shades like black and brown common, as well as more individual colors like blue and green. The solvent ensures even dispersion of the colorant and maintains the fluidity of the liquid eyeliner. The film-forming agent dries quickly after application, forming a thin film that enhances the eyeliner's durability. Additives may include preservatives and stabilizers to ensure product quality and stability.

[0003] Early liquid eyeliners had several drawbacks, such as easy smudging, difficulty controlling the amount used, and a long drying time. To address this issue, researchers introduced waterproof and anti-smudging technology. By using specialized film-forming agents and polymers, they create a strong, durable, waterproof film around the eye, protecting it from sweat, tears, and oil.

[0004] Given that certain ingredients in some commercially available liquid eyeliners come into contact with the base of eyelashes, they can affect the ecological environment there, leading to clogged eyelash pores, eye discomfort, impaired normal eyelash growth, and even eyelash loss, seriously impacting the aesthetic appearance of eyelashes. Furthermore, some people are born with sparse, short, or less dense eyelashes. Alternatively, eyelashes may be damaged in daily life through makeup, improper use of eyelash curlers, perming and dyeing, causing them to become brittle, break, or grow slowly. Therefore, there is a need to develop and improve liquid eyeliner technology, including one that promotes eyelash growth, thereby reducing irritation to the eyes and skin, and potentially harming eyelash health. Such eyeliners can improve the condition of eyelashes, promoting their growth and density while defining the eyeline, helping people achieve a more desirable eyelash appearance. This can visually make eyes appear larger and more vibrant, enhancing the overall appearance of a more lively and attractive person. Furthermore, using such eyeliners can repair and promote eyelash growth, restoring the health of lashes. By incorporating the effect of promoting eyelash growth into liquid eyeliner, people only need to use one product when doing eye makeup, which can not only draw exquisite eyeliner but also nourish eyelashes and promote their growth at the same time, saving time and steps and providing convenience for busy modern life. Summary of the Invention

[0005] The existing eyeliners have irritation to the eyes and skin, impact on the health of eyelashes and potential harm. The present invention provides a natural plant eyeliner containing eyelash growth ingredients and a preparation method thereof. The eyeliner of the present invention contains argania spinosa kernel extract and jojoba seed extract, which are used in combination with other ingredients to make the eyeliner have the effects of promoting eyelash growth, enhancing eyelash vitality, and reducing eyelash shedding; the argania spinosa kernel extract and jojoba seed extract do not affect the makeup-lasting effect of the eyeliner, and the combination of the argania spinosa kernel extract and the jojoba seed extract has a good synergistic effect in promoting eyelash growth, which can greatly increase the length and density of eyelashes, protect eyelashes, reduce damage to cosmetics, not easily fall off, maintain eyelash density, and is safe and has no side effects. The specific technical scheme is as follows:

[0006] A natural plant eyeliner containing eyelash growth ingredients comprises the following raw materials in parts by weight: 2 to 3.5 parts of argania spinosa kernel extract, 1 to 2.5 parts of jojoba seed extract, 22 to 28 parts of a film-forming agent, 6 to 10 parts of a colorant, 4 to 6 parts of a moisturizer, 2 to 5 parts of a dispersant, 0.05 to 0.12 parts of a preservative, and 60 to 80 parts of a solvent; the argania spinosa kernel extract is obtained by fermenting the argania spinosa kernel with Propionibacterium frasseri and Bifidobacterium longum, followed by sequential enzymatic hydrolysis with ficin and seraspinases, to obtain an extract with a molecular weight of less than 2 kDa to 3 kDa; the jojoba seed extract is obtained by fermenting the jojoba seeds with Propionibacterium frasseri and Bifidobacterium longum, followed by sequential enzymatic hydrolysis with bromelain and nattokinase, to obtain an extract with a molecular weight of less than 2 kDa to 3 kDa.

[0007] In the above-mentioned liquid eyeliner, the preparation method of the Argania spinosa kernel extract comprises the following steps:

[0008] The kernels of the Argania spinosa tree are crushed, and water 8 to 10 times the mass of the Argania spinosa kernel is added to obtain a base liquid; glucose, peptone, potassium dihydrogen phosphate and magnesium sulfate are added to the base liquid to adjust the pH to 6.5 to 6.8, and 0.5 to 1% by mass of the Argania spinosa kernel of Propionibacterium freudenreichii and Bifidobacterium longum are added respectively, and anaerobic fermentation is carried out at 36 to 38°C for 25 to 30 hours, and the pH is adjusted to 5.5 to 6.5, the temperature is adjusted to 60 to 65°C, and 0.5 to 1.5% by mass of the Argania spinosa kernel of Fig is added. Protease is enzymatically hydrolyzed for 50 to 80 minutes, sterilized and enzyme-inactivated, the pH is adjusted to 8.5 to 9.5, the temperature is adjusted to 37° C. to 40° C., 0.5% to 1.5% of the mass of the Argania spinosa kernel by Serratia protease is added, enzymatically hydrolyzed for 60 to 90 minutes, enzyme is inactivated, the pH is adjusted to 6.8 to 7.2, and sieve filtering is performed to obtain a crude filtrate, the crude filtrate is filtered through a 2 kDa to 3 kDa ultrafiltration membrane to obtain an ultrafiltrate below 2 kDa to 3 kDa, and the ultrafiltrate is freeze-dried to obtain the Argania spinosa kernel extract.

[0009] In the above-mentioned liquid eyeliner, the preparation method of the jojoba seed extract comprises the following steps:

[0010] Jojoba seeds are crushed, and water 8 to 10 times the mass of the jojoba seeds is added to obtain a base liquid; glucose, peptone, potassium dihydrogen phosphate and magnesium sulfate are added to the base liquid to adjust the pH to 6.5 to 6.8, and 0.5% to 1% by mass of the jojoba seeds of Propionibacterium freudenreichii and Bifidobacterium longum are added respectively, and anaerobic fermentation is carried out at 36°C to 38°C for 25 to 30 hours, and the pH is adjusted to 6.0 to 7.5, and the temperature is adjusted to 50°C to 60°C, and 0.5% to 1.5% by mass of the jojoba seeds of pineapple are added. The invention discloses a method for preparing jojoba seed extract by enzymolysis of jojoba seed protease for 60 to 120 minutes, sterilizing and inactivating the enzyme, adjusting the pH to 8.0 to 9.0, and adjusting the temperature to 40° C. to 50° C., adding 1% to 3% by weight of jojoba seed nattokinase, and performing enzymolysis for 90 to 150 minutes, inactivating the enzyme, and adjusting the pH to 6.8 to 7.2. The extract is filtered through a mesh to obtain a crude filtrate, and the crude filtrate is filtered through a 2 kDa to 3 kDa ultrafiltration membrane to obtain an ultrafiltrate below 2 kDa to 3 kDa, and freeze-drying to obtain a jojoba seed extract.

[0011] In the above preparation method, the amount of glucose added is 0.05% to 0.15% of the mass of the base liquid; the amount of peptone added is 0.03% to 0.08% of the mass of the base liquid; the amount of potassium dihydrogen phosphate added is 0.005% to 0.008% of the mass of the base liquid; the amount of magnesium sulfate added is 0.003% to 0.005% of the mass of the base liquid; the temperature for sterilization and enzyme inactivation is 80°C to 90°C, and the time for sterilization and enzyme inactivation is 20min to 30min; the temperature for enzyme inactivation is 70°C to 80°C, and the time for enzyme inactivation is 15min to 25min; the mesh size of the sieve is 200mesh to 300mesh.

[0012] In the above-mentioned liquid eyeliner, the film-forming agent is one or more combinations of styrene-acrylate copolymer, carboxymethyl cellulose, hydroxyethyl cellulose, polyvinyl pyrrolidone, acrylate-ethylhexyl acrylate copolymer, and acrylate-dimethylaminoethyl methacrylate copolymer; the colorant is one or more combinations of carbon black, iron red, iron yellow, iron black, and titanium dioxide.

[0013] In the above-mentioned liquid eyeliner, the liquid eyeliner further comprises a preservative, and the preservative is one or more combinations of phenoxyethanol, potassium sorbate, methylparaben, and propylparaben.

[0014] In the above-mentioned liquid eyeliner, the moisturizing agent includes propylene glycol, butylene glycol and sodium hyaluronate, and the mass ratio is: propylene glycol:butylene glycol:sodium hyaluronate = (1-3):(1-3):(0.05-0.1).

[0015] In the above-mentioned liquid eyeliner, the dispersant includes sodium polyacrylate and sodium lignin sulfonate, and the mass ratio is: sodium polyacrylate: sodium lignin sulfonate = (5-8): (1-1.5).

[0016] In the above-mentioned liquid eyeliner, the solvent is purified water.

[0017] The method for preparing the above-mentioned natural plant eyeliner containing eyelash growth ingredients comprises the following steps:

[0018] The solvent is heated to 75° C. to 85° C. by mass, and then a dispersant is added and mixed evenly, followed by a colorant, a moisturizer and a film-forming agent, and mixed evenly. The solvent is cooled to 38° C. to 45° C., and the argania spinosa kernel extract, jojoba seed extract and a preservative are added and mixed evenly to obtain the eyeliner liquid.

[0019] The present invention provides a natural plant eyeliner containing eyelash growth ingredients and a preparation method thereof. Compared with the prior art, the beneficial effects are as follows:

[0020] 1. The eyeliner of the present invention contains argania spinosa kernel extract and jojoba seed extract, which are used in combination with other ingredients to promote eyelash growth, enhance eyelash vitality, and reduce eyelash shedding.

[0021] The Argania spinulosa kernel extract of the present invention is obtained by co-fermenting Argania spinulosa kernels with Propionibacterium fischerii and Bifidobacterium longum, followed by sequential enzymatic hydrolysis with ficin and Serratia protease, to obtain an extract with a molecular weight of less than 2 kDa to 3 kDa. The extract is rich in small molecule peptides with specific efficacy and other small molecule nutrients, is easily absorbed, protects pores, and has the following eyelash growth-promoting effects:

[0022] (1) Promote eyelash growth: Provide sufficient nutrients to eyelash follicles to support the growth and division of eyelash cells; protect hair follicle cells from free radical damage and maintain normal cell metabolism and growth function.

[0023] (2) Stimulate growth factors: stimulate hair follicle cells to produce growth factors, thereby promoting cell proliferation and differentiation, and accelerating the growth rate of eyelashes.

[0024] (3) Increase eyelash density: Activate dormant eyelash follicles and re-enter the growth cycle. This increases the number of eyelashes and thus increases eyelash density. The ingredients in the extract can promote the regeneration and development of hair follicles by regulating the activity of hair follicle stem cells.

[0025] (4) Enhance hair follicle health: Improve the microenvironment of eyelash follicles and enhance the health of hair follicles. Healthy hair follicles can better support the growth of eyelashes, making eyelashes thicker.

[0026] (5) Reduce eyelash shedding: Enhances the structural stability of eyelash follicles, making them more firmly fixed. The small molecule peptide components in the extract help promote the synthesis of structural proteins such as collagen and elastin by hair follicle cells, increasing the strength and toughness of hair follicles. It can prolong the growth period of eyelashes and shorten the resting period, thereby reducing the natural shedding of eyelashes.

[0027] 3. The jojoba seed extract of the present invention is obtained by co-fermenting jojoba seeds with Propionibacterium philippe and Bifidobacterium longum, followed by stepwise enzymatic hydrolysis with bromelain and nattokinase to obtain an extract with a molecular weight of less than 2 kDa to 3 kDa. The extract is rich in effective small molecule peptides and other water-soluble small molecule nutrients, is easily absorbed and utilized, and has the following eyelash growth-promoting effects:

[0028] (1) Promote eyelash growth: Jojoba seed extract is rich in nutrients such as vitamins, minerals, and unsaturated fatty acids. It can promote the growth and division of eyelash cells, protect hair follicle cells from free radical damage, and maintain normal cell metabolism. It can promote eyelash growth by affecting the activity of hormone receptors or regulating the synthesis and metabolism of hormones.

[0029] (2) Increase eyelash density: Jojoba seed extract can activate dormant hair follicle stem cells, causing them to differentiate into new eyelash cells, thereby increasing the number and density of eyelashes.

[0030] (3) Improve the hair follicle microenvironment: It helps to improve the microenvironment around the eyelash follicles, including increasing blood circulation, providing sufficient oxygen and nutrients, and regulating pH value. A good hair follicle microenvironment is conducive to the growth and development of eyelashes and increases the density of eyelashes.

[0031] (4) Enhance the adhesion of hair follicles: The ingredients in the extract can enhance the adhesion between eyelash follicles and surrounding tissues, reducing the loss of eyelashes due to external forces or chemicals.

[0032] (5) Regulate the hair cycle: By regulating the active function of eyelash pores, prolonging the growth period, shortening the resting period and shedding period, thereby reducing the natural shedding of eyelashes.

[0033] Fourth, the addition of a small amount of argania spinosa kernel extract and jojoba seed extract in the present invention does not affect the long-lasting effect of the eyeliner. The combination of argania spinosa kernel extract and jojoba seed extract has a good synergistic effect in promoting eyelash growth, which can significantly increase the length and density of eyelashes, protect eyelashes, reduce damage to cosmetics, prevent eyelashes from falling off, maintain eyelash density, and is safe and has no side effects. DETAILED DESCRIPTION

[0034] The present invention will be further described below with reference to specific implementation cases, but the present invention is not limited to these embodiments.

[0035] Example 1

[0036] A natural plant-based liquid eyeliner containing eyelash growth ingredients, comprising the following raw materials by weight: 2.8 parts Argania spinosa kernel extract, 1.6 parts Jojoba seed extract, 25 parts film-forming agent, 8 parts colorant, 5 parts humectant, 3.5 parts dispersant, 0.12 parts preservative, and 70 parts solvent. The film-forming agent is styrene-acrylate copolymer; the colorant is carbon black; the humectants include propylene glycol, butylene glycol, and sodium hyaluronate in a mass ratio of propylene glycol:butylene glycol:sodium hyaluronate = 2:2:0.07; the dispersant includes sodium polyacrylate and sodium lignin sulfonate in a mass ratio of sodium polyacrylate:sodium lignin sulfonate = 7:1.2; and the solvent is purified water.

[0037] The preparation method of the Argania spinosa kernel extract comprises the following steps:

[0038] The kernels of the Argania spinosa tree are crushed, and water (9 times the mass of the Argania spinosa tree kernel) is added to obtain a base liquid; 0.1% glucose (by mass of the base liquid), 0.05% peptone (by mass of the base liquid), 0.006% potassium dihydrogen phosphate (by mass of the base liquid), and 0.004% magnesium sulfate (by mass of the base liquid) are added to the base liquid, and the pH is adjusted to 6.6; 0.8% Propionibacterium freudenreichii and 0.8% Bifidobacterium longum (by mass of the Argania spinosa kernel) are added respectively, and anaerobic fermentation is carried out at 37°C for 28 hours, and the pH and temperature are adjusted to 6 and 62°C. 1% of the mass of Ficin by weight of the Argania spinulosa kernel was added, and enzymatic hydrolysis was carried out for 65 minutes. The mixture was sterilized and inactivated at 85°C for 25 minutes. The pH was adjusted to 9 and the temperature was adjusted to 38°C. 1% of the mass of Serratia marcescens by weight of the Argania spinulosa kernel was added, and enzymatic hydrolysis was carried out for 75 minutes. The mixture was inactivated at 75°C for 20 minutes. The pH was adjusted to 7 and filtered through a 250-mesh sieve to obtain a crude filtrate. The crude filtrate was filtered through a 2 kDa ultrafiltration membrane to obtain an ultrafiltrate below 2 kDa. The ultrafiltrate was freeze-dried to obtain an Argania spinulosa kernel extract.

[0039] The preparation method of jojoba seed extract comprises the following steps:

[0040] Jojoba seeds were crushed, and water (9 times the mass of the jojoba seeds) was added to obtain a base liquid; 0.1% glucose (mass of the base liquid), 0.05% peptone (mass of the base liquid), 0.006% potassium dihydrogen phosphate (mass of the base liquid), and 0.004% magnesium sulfate (mass of the base liquid) were added to the base liquid to adjust the pH to 6.7; 0.8% Propionibacterium freudenreichii and Bifidobacterium longum (mass of the jojoba seeds) were added, and anaerobic fermentation was carried out at 37°C for 28 hours, and the pH was adjusted to 6.8. The temperature was adjusted to 55°C. , add 1% bromelain by weight of jojoba seeds, carry out enzymatic hydrolysis for 90 minutes, sterilize and inactivate the enzyme at 85°C for 25 minutes, adjust the pH to 8.5, adjust the temperature to 45°C, add 2% nattokinase by weight of jojoba seeds, carry out enzymatic hydrolysis for 120 minutes, inactivate the enzyme at 75°C for 20 minutes, adjust the pH to 7, filter through a 250-mesh sieve to obtain a crude filtrate, filter the crude filtrate through a 2 kDa ultrafiltration membrane to obtain an ultrafiltrate below 2 kDa, and freeze-dry to obtain a jojoba seed extract.

[0041] The method for preparing the above-mentioned natural plant eyeliner containing eyelash growth ingredients comprises the following steps:

[0042] According to the mass ratio, the solvent was heated to 80° C., and then a dispersant was added and mixed evenly, followed by adding a colorant and mixing evenly, and then a moisturizer and a film-forming agent were added and mixed evenly, and the mixture was cooled to 42° C., and the argania spinosa kernel extract, jojoba seed extract and preservative were added and mixed evenly to obtain the eyeliner liquid.

[0043] The bacterial activity of Propionibacterium freudenreichii used in this example was 2 billion CFU / g; the bacterial activity of Bifidobacterium longum was 1 billion CFU / g; the enzymatic activity of ficin was 100,000 U / g; the enzymatic activity of Serratia marcescens was 200,000 U / g; the enzymatic activity of bromelain was 500,000 U / g; and the enzymatic activity of nattokinase was 10,000 U / g. Phenoxyethanol was used as the preservative.

[0044] Example 2

[0045] A natural plant-based liquid eyeliner containing eyelash growth ingredients, comprising the following raw materials in parts by weight: 2 parts Argania spinosa kernel extract, 1 part Jojoba seed extract, 22 parts film-forming agent, 6 parts colorant, 4 parts humectant, 2 parts dispersant, 0.08 parts preservative, and 60 parts solvent. The film-forming agent is styrene-acrylate copolymer; the colorant is carbon black; the humectants include propylene glycol, butylene glycol, and sodium hyaluronate in a mass ratio of propylene glycol:butylene glycol:sodium hyaluronate = 1:1:0.05; the dispersant includes sodium polyacrylate and sodium lignin sulfonate in a mass ratio of sodium polyacrylate:sodium lignin sulfonate = 5:1; and the solvent is purified water.

[0046] The preparation method of the Argania spinosa kernel extract comprises the following steps:

[0047] The kernels of the Argania spinosa tree are crushed, and water 8 times the mass of the Argania spinosa tree kernel is added to obtain a base liquid; 0.05% of glucose by mass of the base liquid, 0.03% of peptone by mass of the base liquid, 0.005% of potassium dihydrogen phosphate by mass of the base liquid, and 0.003% of magnesium sulfate by mass of the base liquid are added to the base liquid, and the pH is adjusted to 6.5. 0.5% of Propionibacterium freudenreichii and Bifidobacterium longum by mass of the Argania spinosa kernel are added respectively, and anaerobic fermentation is carried out at 36°C for 25 hours, the pH is adjusted to 5.5, the temperature is adjusted to 60°C, and the addition of The method comprises the following steps: adding 0.5% of the mass of Ficin by weight of the Argania spinulosa kernel, performing enzymatic hydrolysis for 50 minutes, sterilizing and inactivating the enzyme at 80°C for 20 minutes, adjusting the pH to 8.5, and adjusting the temperature to 37°C; adding 0.5% of the mass of Serratia marcescens protease by weight of the Argania spinulosa kernel, performing enzymatic hydrolysis for 60 minutes, inactivating the enzyme at 70°C for 15 minutes, adjusting the pH to 6.8, filtering through a 200-mesh sieve to obtain a crude filtrate; filtering the crude filtrate through a 2 kDa ultrafiltration membrane to obtain an ultrafiltrate below 2 kDa, and freeze-drying to obtain an Argania spinulosa kernel extract.

[0048] The preparation method of jojoba seed extract comprises the following steps:

[0049] Jojoba seeds were crushed, and water 8 times the mass of the jojoba seeds was added to obtain a base liquid; 0.05% of glucose by mass of the base liquid, 0.03% of peptone by mass of the base liquid, 0.005% of potassium dihydrogen phosphate by mass of the base liquid, and 0.003% of magnesium sulfate by mass of the base liquid were added to the base liquid, and the pH was adjusted to 6.5; 0.5% of Propionibacterium freudenreichii and Bifidobacterium longum by mass of the jojoba seeds were added respectively, and anaerobic fermentation was carried out at 36°C for 25 hours, and the pH was adjusted to 6.0. The temperature was adjusted to 50°C. 0.5% bromelain by weight of jojoba seeds was added, and enzymatic hydrolysis was carried out for 60 minutes. The mixture was sterilized and inactivated at 80° C. for 20 minutes, the pH was adjusted to 8.0, and the temperature was adjusted to 40° C. 1% nattokinase by weight of jojoba seeds was added, and enzymatic hydrolysis was carried out for 90 minutes. The mixture was inactivated at 70° C. for 15 minutes, and the pH was adjusted to 6.8. The mixture was filtered through a 200-mesh sieve to obtain a crude filtrate. The crude filtrate was filtered through a 2 kDa ultrafiltration membrane to obtain an ultrafiltrate below 2 kDa, and the ultrafiltrate was freeze-dried to obtain a jojoba seed extract.

[0050] The method for preparing the above-mentioned natural plant eyeliner containing eyelash growth ingredients comprises the following steps:

[0051] According to the mass ratio, the solvent is heated to 75° C., and then a dispersant is added and mixed evenly, followed by adding a colorant and mixing evenly, followed by adding a moisturizer and a film-forming agent and mixing evenly, and the mixture is cooled to 38° C., and the argania spinosa kernel extract, jojoba seed extract and preservative are added and mixed evenly to obtain the eyeliner liquid.

[0052] The bacterial activity of Propionibacterium freudenreichii used in this example was 2 billion CFU / g; the bacterial activity of Bifidobacterium longum was 1 billion CFU / g; the enzymatic activity of ficin was 100,000 U / g; the enzymatic activity of Serratia marcescens was 200,000 U / g; the enzymatic activity of bromelain was 500,000 U / g; and the enzymatic activity of nattokinase was 10,000 U / g. The preservative was methylparaben.

[0053] Example 3

[0054] A natural plant-based liquid eyeliner containing eyelash growth ingredients, comprising the following raw materials by weight: 3.5 parts Argania spinosa kernel extract, 2.5 parts Jojoba seed extract, 28 parts film-forming agent, 10 parts colorant, 6 parts humectant, 5 parts dispersant, 0.05 parts preservative, and 80 parts solvent. The film-forming agent is styrene-acrylate copolymer; the colorant is carbon black; the humectants include propylene glycol, butylene glycol, and sodium hyaluronate in a mass ratio of propylene glycol:butylene glycol:sodium hyaluronate = 3:3:0.1; the dispersant includes sodium polyacrylate and sodium lignin sulfonate in a mass ratio of sodium polyacrylate:sodium lignin sulfonate = 8:1.5; and the solvent is purified water.

[0055] The preparation method of the Argania spinosa kernel extract comprises the following steps:

[0056] The kernels of the spiny argan tree were crushed, and water 10 times the mass of the kernels was added to obtain a base liquid; 0.15% of glucose by mass of the base liquid, 0.08% of peptone by mass of the base liquid, 0.008% of potassium dihydrogen phosphate by mass of the base liquid, and 0.005% of magnesium sulfate by mass of the base liquid were added to the base liquid, and the pH was adjusted to 6.8. Propionibacterium freudenreichii and Bifidobacterium longum by mass of 1% of the mass of the kernels were added respectively, and anaerobic fermentation was carried out at 38°C for 30h, and the pH was adjusted to 6.5, the temperature was adjusted to 65°C, and the argan tree was added. The method comprises the following steps: adding 1.5% of the mass of Ficin by weight of the Argania spinosa kernel, performing enzymatic hydrolysis for 80 minutes, sterilizing and inactivating the enzyme at 90°C for 30 minutes, adjusting the pH to 9.5, and adjusting the temperature to 40°C; adding 1.5% of the mass of Serratia marcescens by weight of the Argania spinosa kernel, performing enzymatic hydrolysis for 90 minutes, inactivating the enzyme at 80°C for 25 minutes, adjusting the pH to 7.2, and filtering through a 300-mesh sieve to obtain a crude filtrate; filtering the crude filtrate through a 3 kDa ultrafiltration membrane to obtain an ultrafiltrate below 3 kDa, and freeze-drying to obtain an Argania spinosa kernel extract.

[0057] The preparation method of jojoba seed extract comprises the following steps:

[0058] Jojoba seeds were crushed, and water 10 times the mass of the jojoba seeds was added to obtain a base liquid; 0.15% of glucose by mass of the base liquid, 0.08% of peptone by mass of the base liquid, 0.008% of potassium dihydrogen phosphate by mass of the base liquid, and 0.005% of magnesium sulfate by mass of the base liquid were added to the base liquid, and the pH was adjusted to 6.8. Propionibacterium freudenreichii and Bifidobacterium longum by mass of 1% of the jojoba seeds were added respectively, and anaerobic fermentation was carried out at 38°C for 30h, and the pH was adjusted to 7.5. The temperature was adjusted to 60°C, and the temperature was increased. 1.5% bromelain by weight of jojoba seeds was added, and enzymatic hydrolysis was carried out for 120 minutes. The mixture was sterilized and inactivated at 90° C. for 30 minutes. The pH was adjusted to 9.0 and the temperature was adjusted to 50° C. 3% nattokinase by weight of jojoba seeds was added, and enzymatic hydrolysis was carried out for 150 minutes. The mixture was inactivated at 80° C. for 25 minutes. The pH was adjusted to 7.2. The mixture was filtered through a 300-mesh sieve to obtain a crude filtrate. The crude filtrate was filtered through a 3 kDa ultrafiltration membrane to obtain an ultrafiltrate below 3 kDa. The ultrafiltrate was freeze-dried to obtain a jojoba seed extract.

[0059] The method for preparing the above-mentioned natural plant eyeliner containing eyelash growth ingredients comprises the following steps:

[0060] The solvent was heated to 85°C according to the mass ratio, and then a dispersant was added and mixed evenly. Then, a colorant was added and mixed evenly. Then, a moisturizer and a film-forming agent were added and mixed evenly. The solution was cooled to 45°C, and the Argania spinosa kernel extract, the jojoba seed extract, and a preservative were added and mixed evenly to obtain a liquid eyeliner. The preservative was propylparaben.

[0061] The bacterial activity of Propionibacterium freudenreichii used in this embodiment is 3 billion CFU / g; the bacterial activity of Bifidobacterium longum is 2 billion CFU / g; the enzymatic activity of ficin is 100,000 U / g; the enzymatic activity of Serratia protease is 200,000 U / g; the enzymatic activity of bromelain is 500,000 U / g; and the enzymatic activity of nattokinase is 10,000 U / g.

[0062] Example 4

[0063] A natural plant-based liquid eyeliner containing eyelash growth ingredients, comprising the following raw materials by weight: 2 parts Argania spinosa kernel extract, 2.5 parts Jojoba seed extract, 22 parts film-forming agent, 10 parts colorant, 4 parts humectant, 5 parts dispersant, 0.1 parts preservative, and 60 parts solvent. The film-forming agent is styrene-acrylate copolymer; the colorant is carbon black; the humectants include propylene glycol, butylene glycol, and sodium hyaluronate in a mass ratio of propylene glycol:butylene glycol:sodium hyaluronate = 3:1:0.1; the dispersant includes sodium polyacrylate and sodium lignin sulfonate in a mass ratio of sodium polyacrylate:sodium lignin sulfonate = 5:1.5; and the solvent is purified water.

[0064] The preparation method of the Argania spinosa kernel extract comprises the following steps:

[0065] The kernels of the Argania spinosa tree were crushed, and water 8 times the mass of the Argania spinosa tree kernel was added to obtain a base liquid; 0.15% of glucose by mass of the base liquid, 0.03% of peptone by mass of the base liquid, 0.008% of potassium dihydrogen phosphate by mass of the base liquid, and 0.003% of magnesium sulfate by mass of the base liquid were added to the base liquid, and the pH was adjusted to 6.8. Propionibacterium freudenreichii and Bifidobacterium longum by mass of 0.5% of the mass of the Argania spinosa kernel were added respectively, and anaerobic fermentation was carried out at 38°C for 25h, and the pH was adjusted to 6.5, the temperature was adjusted to 60°C, and the addition of 1.5% of the mass of Ficin by weight of the Argania spinulosa kernel was added, and enzymatic hydrolysis was carried out for 50 minutes. The mixture was sterilized and inactivated at 90°C for 20 minutes, the pH was adjusted to 9.5, the temperature was adjusted to 37°C, 1.5% of the mass of Serratia marcescens protease by weight of the Argania spinulosa kernel was added, and enzymatic hydrolysis was carried out for 60 minutes. The enzyme was inactivated at 80°C for 15 minutes, the pH was adjusted to 7.2, and the mixture was filtered through a 200-mesh sieve to obtain a crude filtrate. The crude filtrate was filtered through a 3 kDa ultrafiltration membrane to obtain an ultrafiltrate below 3 kDa, which was freeze-dried to obtain an Argania spinulosa kernel extract.

[0066] The preparation method of jojoba seed extract comprises the following steps:

[0067] Jojoba seeds were crushed, and water 8 times the mass of the jojoba seeds was added to obtain a base liquid; 0.15% of glucose by mass of the base liquid, 0.03% of peptone by mass of the base liquid, 0.008% of potassium dihydrogen phosphate by mass of the base liquid, and 0.003% of magnesium sulfate by mass of the base liquid were added to the base liquid, and the pH was adjusted to 6.8. Propionibacterium freudenreichii and Bifidobacterium longum by mass of 0.5% of the mass of the jojoba seeds were added respectively, and anaerobic fermentation was carried out at 38°C for 25 hours, and the pH was adjusted to 7.5. The temperature was adjusted to 50°C. 1.5% bromelain by weight of jojoba seeds was added, and enzymatic hydrolysis was carried out for 60 minutes. The mixture was sterilized and inactivated at 90° C. for 20 minutes, the pH was adjusted to 9.0, the temperature was adjusted to 40° C., 3% nattokinase by weight of jojoba seeds was added, and enzymatic hydrolysis was carried out for 90 minutes. The mixture was inactivated at 80° C. for 15 minutes, the pH was adjusted to 7.2, and the mixture was filtered through a 200-mesh sieve to obtain a crude filtrate. The crude filtrate was filtered through a 3 kDa ultrafiltration membrane to obtain an ultrafiltrate below 3 kDa, and the ultrafiltrate was freeze-dried to obtain a jojoba seed extract.

[0068] The method for preparing the above-mentioned natural plant eyeliner containing eyelash growth ingredients comprises the following steps:

[0069] The solvent is heated to 85°C according to the mass ratio, and then a dispersant is added and mixed evenly. Then, a colorant is added and mixed evenly. Then, a moisturizer and a film-forming agent are added and mixed evenly. The solution is cooled to 38°C, and the Argania spinosa kernel extract, the jojoba seed extract, and a preservative are added and mixed evenly to obtain a liquid eyeliner. The preservative is potassium sorbate.

[0070] The bacterial activity of Propionibacterium freudenreichii used in this embodiment is 3 billion CFU / g; the bacterial activity of Bifidobacterium longum is 1 billion CFU / g; the enzymatic activity of ficin is 100,000 U / g; the enzymatic activity of Serratia marcescens is 200,000 U / g; the enzymatic activity of bromelain is 500,000 U / g; and the enzymatic activity of nattokinase is 10,000 U / g.

[0071] Example 5

[0072] A natural plant-based liquid eyeliner containing eyelash growth ingredients, comprising the following raw materials by weight: 3.5 parts Argania spinosa kernel extract, 1 part Jojoba seed extract, 28 parts film-forming agent, 6 parts colorant, 6 parts humectant, 2 parts dispersant, 0.08 parts preservative, and 80 parts solvent. The film-forming agent is styrene-acrylate copolymer; the colorant is carbon black; the humectants include propylene glycol, butylene glycol, and sodium hyaluronate in a mass ratio of propylene glycol:butylene glycol:sodium hyaluronate = 1:3:0.05; the dispersant includes sodium polyacrylate and sodium lignin sulfonate in a mass ratio of sodium polyacrylate:sodium lignin sulfonate = 8:1; and the solvent is purified water.

[0073] The preparation method of the Argania spinosa kernel extract comprises the following steps:

[0074] The kernels of the spiny argan tree were crushed, and water 10 times the mass of the kernels was added to obtain a base liquid; 0.05% of glucose by mass of the base liquid, 0.08% of peptone by mass of the base liquid, 0.005% of potassium dihydrogen phosphate by mass of the base liquid, and 0.005% of magnesium sulfate by mass of the base liquid were added to the base liquid, and the pH was adjusted to 6.5. Propionibacterium freudenreichii and Bifidobacterium longum by mass of 1% of the mass of the argan tree kernel were added respectively, and anaerobic fermentation was carried out at 36°C for 30h, and the pH was adjusted to 5.5, the temperature was adjusted to 65°C, and the argan tree was added. The method comprises the following steps: adding 0.5% of the mass of Ficin by weight of the Argania spinosa kernel, performing enzymatic hydrolysis for 80 minutes, sterilizing and inactivating the enzyme at 80°C for 30 minutes, adjusting the pH to 8.5, and adjusting the temperature to 40°C; adding 0.5% of the mass of Serratia marcescens by weight of the Argania spinosa kernel protease, performing enzymatic hydrolysis for 90 minutes, inactivating the enzyme at 70°C for 25 minutes, adjusting the pH to 6.8, and filtering through a 300-mesh sieve to obtain a crude filtrate; filtering the crude filtrate through a 2 kDa ultrafiltration membrane to obtain an ultrafiltrate below 2 kDa, and freeze-drying to obtain an Argania spinosa kernel extract.

[0075] The preparation method of jojoba seed extract comprises the following steps:

[0076] Jojoba seeds were crushed, and water 10 times the mass of the jojoba seeds was added to obtain a base liquid; 0.05% of glucose by mass of the base liquid, 0.08% of peptone by mass of the base liquid, 0.005% of potassium dihydrogen phosphate by mass of the base liquid, and 0.005% of magnesium sulfate by mass of the base liquid were added to the base liquid, and the pH was adjusted to 6.5. Propionibacterium freudenreichii and Bifidobacterium longum by mass of 1% of the jojoba seeds were added respectively, and anaerobic fermentation was carried out at 36°C for 30 hours, and the pH was adjusted to 6.0, the temperature was adjusted to 60°C, and the temperature was increased. 0.5% bromelain by weight of jojoba seeds was added, and enzymatic hydrolysis was carried out for 120 minutes. The mixture was sterilized and inactivated at 80° C. for 30 minutes, the pH was adjusted to 8.0, and the temperature was adjusted to 50° C.; 1% nattokinase by weight of jojoba seeds was added, and enzymatic hydrolysis was carried out for 150 minutes. The mixture was inactivated at 70° C. for 25 minutes, and the pH was adjusted to 6.8. The mixture was filtered through a 300-mesh sieve to obtain a crude filtrate. The crude filtrate was filtered through a 2 kDa ultrafiltration membrane to obtain an ultrafiltrate below 2 kDa, and the ultrafiltrate was freeze-dried to obtain a jojoba seed extract.

[0077] The method for preparing the above-mentioned natural plant eyeliner containing eyelash growth ingredients comprises the following steps:

[0078] According to the mass ratio, the solvent is heated to 75° C., and then a dispersant is added and mixed evenly, followed by adding a colorant and mixing evenly, and then a moisturizer and a film-forming agent are added and mixed evenly, and the mixture is cooled to 45° C., and the argania spinosa kernel extract, jojoba seed extract and preservative are added and mixed evenly to obtain the eyeliner liquid.

[0079] The bacterial activity of Propionibacterium freudenreichii used in this example was 2 billion CFU / g; the bacterial activity of Bifidobacterium longum was 2 billion CFU / g; the enzymatic activity of ficin was 100,000 U / g; the enzymatic activity of Serratia marcescens was 200,000 U / g; the enzymatic activity of bromelain was 500,000 U / g; and the enzymatic activity of nattokinase was 10,000 U / g. Potassium sorbate was used as the preservative.

[0080] Example 6

[0081] The film-forming agent is a mixture of styrene-acrylate copolymer and carboxymethyl cellulose in a mass ratio of 4:1; the colorant is a mixture of titanium dioxide, red iron oxide, yellow iron oxide, and black iron oxide in a mass ratio of 3:4:6:1; the eyeliner also contains 0.12 parts of preservatives such as phenoxyethanol and potassium sorbate in a mass ratio; other parameters and methods are the same as those in Example 1.

[0082] Example 7

[0083] The film-forming agent is a mixture of hydroxyethyl cellulose and acrylate-ethylhexyl acrylate copolymer in a mass ratio of 3:1; the colorant is a mixture of iron oxide red, iron oxide yellow, and iron oxide black in a mass ratio of 2:4:1; the eyeliner also contains 0.1 parts of preservatives such as phenoxyethanol and methylparaben in a mass ratio; other parameters and methods are the same as those in Example 1.

[0084] Example 8

[0085] The film-forming agent is a mixture of polyvinyl pyrrolidone and acrylate-dimethylaminoethyl methacrylate copolymer in a mass ratio of 2:3; the colorant is a mixture of iron oxide black, iron oxide red, and iron oxide yellow in a mass ratio of 0.5:4:1; the eyeliner also contains 0.08 parts of preservatives such as phenoxyethanol and propylparaben in a mass ratio; other parameters and methods are the same as those in Example 1.

[0086] Comparative Example 1

[0087] No Argania spinosa kernel extract was added to the eyeliner, and the content of the Argania spinosa kernel extract was replaced by a solvent; other parameters and methods were the same as in Example 1.

[0088] Comparative Example 2

[0089] In the preparation method of the Argania spinosa kernel extract, Argania spinosa kernels are ground, 9 times the mass of water of the Argania spinosa kernels are added to obtain a base solution; the pH is adjusted to 6, the temperature is adjusted to 62°C, 1% of the mass of ficin by weight of the Argania spinosa kernels is added, enzymatic hydrolysis is carried out for 65 minutes, sterilization is carried out at 85°C for 25 minutes to inactivate the enzyme, the pH is adjusted to 9, the temperature is adjusted to 38°C, 1% of the mass of Serratia marcescens by weight of the Argania spinosa kernels is added, enzymatic hydrolysis is carried out for 75 minutes, the enzyme is inactivated at 75°C for 20 minutes, the pH is adjusted to 7, and the crude filtrate is filtered through a 250-mesh sieve to obtain a coarse filtrate, which is filtered through a 2 kDa ultrafiltration membrane to obtain an ultrafiltrate below 2 kDa, and freeze-dried to obtain the Argania spinosa kernel extract. Other parameters and methods are the same as those in Example 1.

[0090] Comparative Example 3

[0091] In the preparation method of the Argania spinosa kernel extract, Argania spinosa kernels are ground and 9 times the mass of water of the Argania spinosa kernels are added to obtain a base liquid; 0.1% glucose, 0.05% peptone, 0.006% potassium dihydrogen phosphate, and 0.004% magnesium sulfate are added to the base liquid by mass, and the pH is adjusted to 6.6. 0.8% Propionibacterium freudenreichii and Bifidobacterium longum are added, respectively, by mass of the Argania spinosa kernels. Anaerobic fermentation is carried out at 37°C for 28 hours, the pH is adjusted to 7, and the extract is filtered through a 250-mesh sieve to obtain a crude filtrate. The crude filtrate is filtered through a 2 kDa ultrafiltration membrane to obtain an ultrafiltrate with a concentration below 2 kDa, which is then freeze-dried to obtain the Argania spinosa kernel extract. Other parameters and methods are the same as those in Example 1.

[0092] Comparative Example 4

[0093] The preparation method of the Argania spinulosa kernel extract comprises: grinding Argania spinulosa kernels, adding 9 times the mass of water as the mass of the Argania spinulosa kernels to obtain a base liquid; adding 0.1% glucose, 0.05% peptone, 0.006% potassium dihydrogen phosphate, and 0.004% magnesium sulfate as the mass of the base liquid to the base liquid, adjusting the pH to 6.6; adding 0.8% Propionibacterium freudenreichii as the mass of the Argania spinulosa kernels; performing anaerobic fermentation at 37°C for 28 hours, adjusting the pH to 6, and adjusting the temperature to 62°C; adding 1% ficin as the mass of the Argania spinulosa kernels; performing enzymatic hydrolysis for 65 minutes; sterilizing at 85°C for 25 minutes to inactivate the enzyme; adjusting the pH to 7; and filtering through a 250-mesh sieve to obtain a crude filtrate. The crude filtrate is filtered through a 2 kDa ultrafiltration membrane to obtain an ultrafiltrate below 2 kDa, which is freeze-dried to obtain the Argania spinulosa kernel extract. Other parameters and methods are the same as those in Example 1.

[0094] Comparative Example 5

[0095] In the preparation method of the Argania spinulosa kernel extract, Argania spinulosa kernels are ground, 9 times the mass of water of the Argania spinulosa kernels are added to obtain a base liquid; 0.1% glucose, 0.05% peptone, 0.006% potassium dihydrogen phosphate, and 0.004% magnesium sulfate are added to the base liquid, the pH is adjusted to 6.6, 0.8% Bifidobacterium longum by mass of the Argania spinulosa kernels are added, anaerobic fermentation is carried out at 37°C for 28 hours, the pH is adjusted to 9, the temperature is adjusted to 38°C, 1% Serratia protease by mass of the Argania spinulosa kernels is added, enzymatic hydrolysis is carried out for 75 minutes, the enzyme is inactivated at 75°C for 20 minutes, the pH is adjusted to 7, and the crude filtrate is filtered through a 250-mesh sieve to obtain a crude filtrate, which is filtered through a 2 kDa ultrafiltration membrane to obtain an ultrafiltrate below 2 kDa, and freeze-dried to obtain the Argania spinulosa kernel extract. Other parameters and methods are the same as those in Example 1.

[0096] Comparative Example 6

[0097] No jojoba seed extract was added to the eyeliner, and the content of jojoba seed extract was replaced by a solvent; other parameters and methods were the same as in Example 1.

[0098] Comparative Example 7

[0099] In the preparation method of jojoba seed extract, jojoba seeds are crushed, and water 9 times the mass of the jojoba seeds is added to obtain a base liquid; the pH is adjusted to 6.8, the temperature is adjusted to 55°C, 1% of bromelain by mass of the jojoba seeds is added, and enzymatic hydrolysis is carried out for 90 minutes, sterilized at 85°C for 25 minutes to inactivate the enzyme, the pH is adjusted to 8.5, the temperature is adjusted to 45°C, 2% of nattokinase by mass of the jojoba seeds is added, and enzymatic hydrolysis is carried out for 120 minutes, the enzyme is inactivated at 75°C for 20 minutes, the pH is adjusted to 7, and the solution is filtered through a 250-mesh sieve to obtain a crude filtrate, which is filtered through a 2 kDa ultrafiltration membrane to obtain an ultrafiltrate below 2 kDa, and the solution is freeze-dried to obtain the jojoba seed extract. Other parameters and methods are the same as those in Example 1.

[0100] Comparative Example 8

[0101] In the preparation method of jojoba seed extract, jojoba seeds are crushed, and water 9 times the mass of the jojoba seeds is added to obtain a base liquid; 0.1% glucose, 0.05% peptone, 0.006% potassium dihydrogen phosphate, and 0.004% magnesium sulfate are added to the base liquid by mass, and the pH is adjusted to 6.7. 0.8% Propionibacterium freudenreichii and Bifidobacterium longum are added, respectively, by mass of the jojoba seeds. Anaerobic fermentation is carried out at 37° C. for 28 hours, the pH is adjusted to 7, and the mixture is filtered through a 250-mesh sieve to obtain a crude filtrate. The crude filtrate is filtered through a 2 kDa ultrafiltration membrane to obtain an ultrafiltrate below 2 kDa, which is then freeze-dried to obtain the jojoba seed extract. Other parameters and methods are the same as those in Example 1.

[0102] Comparative Example 9

[0103] In the preparation method of jojoba seed extract, jojoba seeds are crushed, 9 times the mass of water of the jojoba seeds are added to obtain a base liquid; 0.1% glucose by mass of the base liquid, 0.05% peptone by mass of the base liquid, 0.006% potassium dihydrogen phosphate by mass of the base liquid, and 0.004% magnesium sulfate by mass of the base liquid are added to the base liquid, the pH is adjusted to 6.7, 0.8% Propionibacterium freudenreichii by mass of the jojoba seeds are added, anaerobic fermentation is carried out at 37°C for 28 hours, the pH is adjusted to 8.5, the temperature is adjusted to 45°C, 2% nattokinase by mass of the jojoba seeds is added, enzymatic hydrolysis is carried out for 120 minutes, the enzyme is inactivated at 75°C for 20 minutes, the pH is adjusted to 7, and the crude filtrate is filtered through a 250-mesh sieve to obtain a crude filtrate, the crude filtrate is filtered through a 2 kDa ultrafiltration membrane to obtain an ultrafiltrate below 2 kDa, and the ultrafiltrate is freeze-dried to obtain the jojoba seed extract. Other parameters and methods are the same as those in Example 1.

[0104] Comparative Example 10

[0105] In the preparation method of jojoba seed extract, jojoba seeds are crushed, 9 times the mass of water of the jojoba seeds are added to obtain a base liquid; 0.1% glucose by mass of the base liquid, 0.05% peptone by mass of the base liquid, 0.006% potassium dihydrogen phosphate by mass of the base liquid, and 0.004% magnesium sulfate by mass of the base liquid are added to the base liquid, the pH is adjusted to 6.7, 0.8% Bifidobacterium longum by mass of the jojoba seeds is added, anaerobic fermentation is carried out at 37° C. for 28 hours, the pH is adjusted to 6.8, the temperature is adjusted to 55° C., 1% bromelain by mass of the jojoba seeds is added, enzymatic hydrolysis is carried out for 90 minutes, sterilization is carried out at 85° C. for 25 minutes to inactivate the enzyme, the pH is adjusted to 7, and the crude filtrate is filtered through a 250-mesh sieve to obtain a crude filtrate, the crude filtrate is filtered through a 2 kDa ultrafiltration membrane to obtain an ultrafiltrate below 2 kDa, and the ultrafiltrate is freeze-dried to obtain the jojoba seed extract. Other parameters and methods are the same as those in Example 1.

[0106] The liquid eyeliners of Examples 1 to 5 and Comparative Examples 1 to 10 were tested.

[0107] 1. Patch test

[0108] Thirty subjects (female aged 20 to 35) were selected, and 0.025g of each liquid eyeliner sample was placed in a spot tester chamber and applied to the inside of the subject's arm. The spot tester was removed after 24 hours, and the skin condition was observed within 48 hours. No adverse reactions were found, and the results showed that the eyeliner was safe.

[0109] 2. Animal Test on Eyelash Growth Promotion

[0110] Several Wi star rats (500g-550g body weight) were selected and randomly divided into 15 groups, with 8 rats in each group (each group used the eyeliner of each embodiment and comparative example respectively). After the rats were anesthetized, the upper eyelashes of both eyes were plucked out. The skin at the root of the upper eyelashes of the left eye of the rat was painted with eyeliner every day, and the skin at the root of the upper eyelashes of the right eye was painted with purified water; once a day for 45 days. The average growth length of the left and right upper eyelashes was measured respectively, and the eyelash growth promotion rate of change was calculated. Eyelash growth promotion rate of change = (average growth length of the left upper eyelash - average growth length of the right upper eyelash) ÷ average growth length of the right upper eyelash × 100%; the test results are shown in Table 1 below.

[0111] Table 1 Eyelash growth promotion rate of change

[0112]

[0113] The above results show that the eyeliners of Examples 1 to 5 have a good effect on promoting eyelash growth in rats. Comparing the results of Comparative Example 1, it can be seen that without the addition of the Argania spinosa kernel extract, the eyelash growth-promoting effect is significantly reduced, while the Argania spinosa kernel extract has a good eyelash growth-promoting effect. Comparing the results of Comparative Examples 2 to 5, it can be seen that changing the preparation method of the Argania spinosa kernel extract and omitting some of the fermentation and enzymatic hydrolysis steps results in different product components, which seriously affects the eyelash growth-promoting effect of the Argania spinosa kernel extract. Comparing the results of Comparative Example 6, it can be seen that without the addition of the jojoba seed extract, the eyelash growth-promoting effect is significantly reduced, while the jojoba seed extract has a good eyelash growth-promoting effect. Comparing the results of Comparative Examples 7 to 10, it can be seen that changing the preparation method of the jojoba seed extract and omitting some of the fermentation and enzymatic hydrolysis steps results in different product components, which seriously affects the eyelash growth-promoting effect of the jojoba seed extract. In addition, by comparing the results of Comparative Examples 1 and 6 with the results of Example 1, it can be seen that the combination of Argania spinosa kernel extract and jojoba seed extract has a good synergistic effect on promoting eyelash growth.

[0114] 3. Human trial on eyelash growth promotion

[0115] 90 subjects were selected. They met the following criteria: relatively short and sparse upper eyelashes, with comparable density and average length for the left and right upper eyelashes, with a relative deviation of ±1%. They were divided into 15 groups, each consisting of 6 subjects (each group used the eyeliner from each example and comparative example). Every morning, the sample eyeliner was applied to the upper lashes of the left eye, while the eyeliner without Argania spinosa kernel extract and Jojoba seed extract (i.e., the eyeliner prepared without Argania spinosa kernel extract and Jojoba seed extract in Example 1) was applied to the upper lashes of the right eye. After three months of use, the density and average length of the left and right upper eyelashes were measured. The rate of change in eyelash growth density was calculated for the left eye relative to the right eye: rate of change = (left eye upper eyelash density - right eye upper eyelash density) ÷ right eye upper eyelash density × 100%. The rate of change in eyelash growth was calculated for the left eye relative to the right eye: rate of change = (left eye upper eyelash density - right eye upper eyelash density) ÷ right eyelash density × 100%. The rate of change in eyelash growth was calculated for the left eye relative to the right eye: rate of change = (left eye upper eyelash average length - right eyelash average length) ÷ right eyelash average length × 100%. The test results are shown in Table 2 below.

[0116] Table 2 Eyelash growth promotion test results

[0117]

[0118] The above results show that the eyeliners of Examples 1 to 5 have a good eyelash growth-promoting effect and can effectively improve the vitality of eyelashes and reduce eyelash shedding. Comparing the results of Comparative Example 1, it can be seen that without the addition of Argania spinosa kernel extract, the eyelash growth-promoting effect is significantly reduced, and the Argania spinosa kernel extract has a good eyelash growth-promoting effect. Comparing the results of Comparative Examples 2 to 5, it can be seen that changing the preparation method of Argania spinosa kernel extract and omitting some fermentation and enzymatic hydrolysis steps results in different product components, which seriously affects the eyelash growth-promoting effect of Argania spinosa kernel extract. Comparing the results of Comparative Example 6, it can be seen that without the addition of jojoba seed extract, the eyelash growth-promoting effect is significantly reduced, and the jojoba seed extract has a good eyelash growth-promoting effect. Comparing the results of Comparative Examples 7 to 10, it can be seen that changing the preparation method of jojoba seed extract and omitting some fermentation and enzymatic hydrolysis steps results in different product components, which seriously affects the eyelash growth-promoting effect of jojoba seed extract. In addition, by comparing the results of Comparative Examples 1 and 6 with the results of Example 1, it can be seen that the combination of Argania spinosa kernel extract and jojoba seed extract has a good synergistic effect on promoting eyelash growth.

Claims

1. A natural plant eyeliner containing eyelash growth ingredients, characterized in that: The eyeliner comprises the following raw materials in parts by weight: 2 to 3.5 parts of Argania spinosa kernel extract, 1 to 2.5 parts of jojoba seed extract, 22 to 28 parts of a film-forming agent, 6 to 10 parts of a colorant, 4 to 6 parts of a moisturizing agent, 2 to 5 parts of a dispersant, 0.05 to 0.12 parts of a preservative, and 60 to 80 parts of a solvent; the solvent is purified water; The preparation method of the Argania spinosa kernel extract comprises the following steps: grinding Argania spinosa kernels, adding water 8 to 10 times the mass of the Argania spinosa kernels to obtain a base liquid; adding glucose, peptone, potassium dihydrogen phosphate and magnesium sulfate to the base liquid, adjusting the pH to 6.5 to 6.8, adding 0.5% to 1% of the mass of the Argania spinosa kernels of Propionibacterium freudenreichii and Bifidobacterium longum, respectively, performing anaerobic fermentation at 36°C to 38°C for 25 to 30 hours, adjusting the pH to 5.5 to 6.5, adjusting the temperature to 60°C to 65°C, adding 0. 5% to 1.5% of ficin is added, and enzymatic hydrolysis is performed for 50 to 80 minutes, followed by sterilization and enzyme inactivation, and the pH is adjusted to 8.5 to 9.5, and the temperature is adjusted to 37°C to 40°C. 0.5% to 1.5% of the mass of the Argania spinosa kernel by weight of Serratia marcescens protease is added, and enzymatic hydrolysis is performed for 60 to 90 minutes, enzyme inactivation is performed, and the pH is adjusted to 6.8 to 7.

2. The crude filtrate is filtered through a 2 kDa to 3 kDa ultrafiltration membrane to obtain an ultrafiltrate below 2 kDa to 3 kDa, and the ultrafiltrate is freeze-dried to obtain an Argania spinosa kernel extract. The preparation method of the jojoba seed extract comprises the following steps: grinding jojoba seeds, adding water 8 to 10 times the mass of the jojoba seeds to obtain a base liquid; adding glucose, peptone, potassium dihydrogen phosphate and magnesium sulfate to the base liquid, adjusting the pH to 6.5 to 6.8, adding 0.5% to 1% by mass of jojoba seeds of Propionibacterium freudenreichii and Bifidobacterium longum, respectively, performing anaerobic fermentation at 36°C to 38°C for 25 to 30 hours, adjusting the pH to 6.0 to 7.5, adjusting the temperature to 50°C to 60°C, adding 0.5% to 1% by mass of jojoba seeds of Propionibacterium freudenreichii and Bifidobacterium longum, and performing anaerobic fermentation at 36°C to 38°C for 25 to 30 hours. .5% to 1.5% bromelain, enzymatically hydrolyze for 60 min to 120 min, sterilize and inactivate the enzyme, adjust the pH to 8.0 to 9.0, adjust the temperature to 40°C to 50°C, add 1% to 3% nattokinase by weight of jojoba seeds, enzymatically hydrolyze for 90 min to 150 min, inactivate the enzyme, adjust the pH to 6.8 to 7.2, filter through a mesh to obtain a crude filtrate, filter the crude filtrate through a 2 kDa to 3 kDa ultrafiltration membrane to obtain an ultrafiltrate below 2 kDa to 3 kDa, and freeze-dry to obtain a jojoba seed extract; The film-forming agent is one or more combinations of styrene-acrylate copolymer, carboxymethyl cellulose, hydroxyethyl cellulose, polyvinyl pyrrolidone, acrylate-ethylhexyl acrylate copolymer, and acrylate-dimethylaminoethyl methacrylate copolymer.

2. The natural plant eyeliner containing eyelash growth ingredients according to claim 1, characterized in that: The amount of glucose added is 0.05% to 0.15% of the base liquid mass; the amount of peptone added is 0.03% to 0.08% of the base liquid mass; the amount of potassium dihydrogen phosphate added is 0.005% to 0.008% of the base liquid mass; the amount of magnesium sulfate added is 0.003% to 0.005% of the base liquid mass; the temperature for sterilization and enzyme inactivation is 80°C to 90°C, and the time for sterilization and enzyme inactivation is 20min to 30min; the temperature for enzyme inactivation is 70°C to 80°C, and the time for enzyme inactivation is 15min to 25min; the mesh size of the sieve is 200mesh to 300mesh.

3. The natural plant eyeliner containing eyelash growth ingredients according to claim 1, characterized in that: The colorant is one or more combinations of carbon black, iron red, iron yellow, iron black, and titanium dioxide.

4. The natural plant eyeliner containing eyelash growth ingredients according to claim 1, characterized in that: The eyeliner also includes a preservative, which is one or more combinations of phenoxyethanol, potassium sorbate, methylparaben, and propylparaben.

5. The natural plant eyeliner containing eyelash growth ingredients according to claim 1, characterized in that: The moisturizing agent includes propylene glycol, butylene glycol and sodium hyaluronate, and the mass ratio is: propylene glycol: butylene glycol: sodium hyaluronate = (1-3): (1-3): (0.05-0.1).

6. The natural plant eyeliner containing eyelash growth ingredients according to claim 1, characterized in that: The dispersant includes sodium polyacrylate and sodium lignin sulfonate, and the mass ratio is: sodium polyacrylate: sodium lignin sulfonate = (5-8): (1-1.5).

7. A method for preparing a natural plant eyeliner containing eyelash growth ingredients, for preparing the natural plant eyeliner containing eyelash growth ingredients according to claim 1, characterized in that: The preparation method comprises the following steps: The solvent is heated to 75° C. to 85° C. by mass, and then a dispersant is added and mixed evenly, followed by a colorant, a moisturizer and a film-forming agent, and mixed evenly. The solvent is cooled to 38° C. to 45° C., and the argania spinosa kernel extract, jojoba seed extract and a preservative are added and mixed evenly to obtain the eyeliner liquid.

Images