Decoction extraction method of xanthoceras sorbifolia 4-flavor soup

Abstract

The invention provides a decoction and extraction method of xanthoceras sorbifolia 4-flavor soup. Single-factor investigation is adopted, and the decocting time, the water adding amount and the soaking time are selected as factors; the method comprises the following steps of: establishing an L9 (34) orthogonal experiment by taking the dry extract yield and the contents of gallic acid, gentiopicroside, epicatechin, dihydromyricetin, ellagic acid and berberine as indexes, measuring and preparing the content and the dry extract rate of a test solution, and obtaining the optimal process conditions that the decoction time is 35 minutes, the water addition amount is 40 times of that of the test solution, and the soaking time is 30 minutes. After six index components obtained from the xanthoceras sorbifolia-four-flavor decoction belong to four single medicines of the prescription respectively, the identification result is that gallic acid and ellagic acid are derived from myrobalan; the epicatechin and the dihydromyricetin are derived from xanthoceras sorbifolia; the gentiopicroside is derived from gentiana macrophylla; berberine is derived from cortex phellodendri. When the content of the shiny-leaved yellowhorn wood-four-flavor decoction prescription is measured, the six effective components can become markers, and a new development concept is provided for researching quality standard formulation of the prescription or developing innovative drugs.

Description

Technical Field

[0001] The invention relates to the field of a decoction and extraction method of a Mongolian medicine Xanthoceras sorbifolia-4-flavor soup, and in particular to a decoction and extraction method of a Xanthoceras sorbifolia-4-flavor soup. Background Art

[0002] The Mongolian medicine Singuanmu-4 Decoction is recorded in the ancient Mongolian medicine book "Four Medical Classics" (subsequent volume), "the decoction of Singuanmu, Terminalia chebula, Gentiana macrophylla, and Phellodendron chinense can treat diseases caused by Xie Ri Wu Su fever." It is also recorded in the works "Four Ganlu" and "Gao Shige Meilin Fang", and is mainly used to treat various Xie Ri Wu Su diseases, rheumatoid diseases, rheumatism, and has the proportion and method of taking prescription drugs.

[0003] Decoctions in Chinese and Mongolian medicine are the most widely used traditional dosage forms in clinical practice. Improper operation of the decoction and extraction process will lead to unstable clinical efficacy. Mongolian medicine decoctions are crushed into coarse powder, then sieved, and all the crushed coarse powder is evenly mixed through an 80-mesh sieve to form a powder; generally, the dosage of Mongolian medicine decoctions is small each time the medicine is taken, and the patients generally take 10 ml-100 ml of water decoctions; Mongolian medicine decoctions are generally decocted once. There are two common decoctions for Mongolian patent medicine: decoction soup and soaking soup. Decoction soup is to crush 3g-5g of medicine into coarse powder according to the decoction preparation requirements, put it into 250mL of water or the required liquid, decocted on a slow fire, and evaporate 2 / 3 of the liquid, such as decoction of seven treasures soup. Soaking soup is to put 3g-5g of medicine into 200L of warm boiled water or the required liquid, soak for 8h-10h, remove the residue and take the juice, such as making three-flavor black alum soup.

[0004] At present, no relevant research on this prescription has been found through literature review, and there is no relevant report on how to determine the content of each component. In order to study the quality standard in the future, a method that can solve the ambiguity in the content determination of Mongolian medicine Sendeng-4-flavor soup is urgently needed. Summary of the invention

[0005] The invention aims to provide a method for decocting and extracting Xanthoceras sorbifolia-4-flavor soup.

[0006] The present invention is achieved through the following technical solutions:

[0007] The present invention relates to a decoction and extraction method of Xanthoceras sorbifolia-4-flavor decoction, comprising the following steps: taking a single factor investigation, selecting decoction time, water addition amount and soaking time as factors; taking dry extract yield and gallic acid, gentiopicroside, epicatechin, dihydromyricetin, ellagic acid and berberine contents as indicators, establishing an L9 (34) orthogonal experiment, determining and preparing the content and dry extract rate of a test solution, and obtaining the optimal process conditions as follows: decoction time of 35 minutes, water addition amount of 40 times, and soaking time of 30 minutes; the specific steps are as follows:

[0008] Step 1, preparation of reference solution

[0009] Accurately weigh the reference substance: gentiopicroside 0.79 mg, placed in a 10 mL brown volumetric bottle, gallic acid 0.98 mg, epicatechin 0.75 mg, dihydromyricetin 0.95 mg, ellagic acid 0.66 mg, berberine 0.61 mg, respectively placed in a 5 mL brown volumetric bottle, add methanol to dissolve and dilute to the scale, shake well, and prepare a stock solution;

[0010] Then, accurately measure different amounts of each reference substance from the stock solution, dilute to the mark with methanol, and shake well to obtain reference substance solutions of 79 μg / mL gentiopicroside, 19.6 μg / mL gallic acid, 24 μg / mL epicatechin, 15.2 μg / mL dihydromyricetin, 6.325 μg / mL ellagic acid, and 73.2 μg / mL berberine, respectively;

[0011] Step 2, preparation of test solution

[0012] Accurately weigh 5g of the sample Mongolian medicine Crownwood-4-flavor soup powder, put it in a decoction pot, accurately add 60 times the amount of water, decoct once, soak for 20min, decoction time is 25min, filter with a 200-mesh sieve, collect the filtrate, take 1mL, put it in a 25mL volumetric flask, add methanol to dissolve only the scale, shake well, filter with a 0.22μm microporous filter membrane and take the filtrate to obtain the test solution;

[0013] Step 3, determination of content

[0014] According to the reference solution, gallic acid is 0.5μL, 2.5μL, 5μL, 7.5μL, 10μL, 12.5μL, 15μL; gentiopicroside, epicatechin, dihydromyricetin are 2.5μL, 5μL, 7.5μL, 10μL, 12.5μL, 15μL; ellagic acid, berberine are 2.5μL, 5μL, 7.5μL, 10μL, 12.5μL, 15μL, 17.5μL, 20μL; the injection volume is injected into the high performance liquid chromatograph, measured according to the method, and the peak area is recorded;

[0015] Step 4: Precision test

[0016] Take a sample, prepare the test solution according to the above method, accurately draw 10 μL of the test solution under the chromatographic conditions, inject 6 times in sequence, record the chromatographic peak, and determine the RSD% of the peak area (n=6); this indicates that the instrument has good precision;

[0017] Step 5, stability test

[0018] Take a sample and prepare the test solution according to the above method. Accurately pipette 10 μL of the test solution under the chromatographic conditions, and inject it at 0h, 2h, 4h, 6h, 8h, 10h, 12h, and 24h after preparation. Record the chromatographic peaks and determine the RSD% (n=6) of the peak area; this indicates that the test solution is basically stable within 24h.

[0019] Step 6, Repeatability Test

[0020] Take a sample, prepare the test solution according to the above method, accurately pipette 10 μL of the test solution under the chromatographic conditions, record the chromatographic peak, and determine the RSD% (n=6) of the peak area; this shows that the method has good repeatability.

[0021] Step 7, sample recovery test

[0022] Take 6 samples, 2.5 g each, accurately weigh and add gallic acid, gentiopicroside, epicatechin, dihydromyricetin, ellagic acid and berberine reference substances in a 1:1 ratio to each sample, prepare and determine the test solution in sequence according to the test solution preparation method, and calculate the recovery rate;

[0023] Step 8, sample content determination

[0024] Take a sample, prepare the test solution according to the above method, accurately draw 10 μL of the test solution under the chromatographic conditions, record the peak area, calculate the content and RSD% (n=3);

[0025] Step 9, determination of dry paste yield

[0026] 25 mL of the decoction extract was accurately measured and placed in an evaporating dish (M1) of constant mass, and then dried in a drying oven at 105°C to constant weight, placed in a desiccator to cool, and accurately weighed (M2); dry paste yield = (M2-M1) / m, where m is the total mass of the medicinal materials in the sample to be tested;

[0027] Step 10, choose the cooking time

[0028] Weigh 5g of the powder of Crownwood-4 Decoction, put it in a decoction pot, accurately add 60 times the amount of water, decoct once, soak for 20 minutes, and the decoction time is 5 minutes, 15 minutes, 25 minutes, and 35 minutes respectively. Filter with a 200-mesh sieve, collect the filtrate, and determine the various indicators and dry paste rate according to the above method; the results show that the increase in decoction time increases the content of various indicators and the dry paste rate, so the minimum decoction time is abandoned, and 15 minutes, 25 minutes, and 35 minutes are selected for subsequent experiments;

[0029] Step 11, choose the amount of water to add

[0030] Weigh 5g of the powder of Crownwood-4 Decoction, put it in a decoction pot, decoct once, soak for 20min, decoct for 35min, accurately add water at 40, 60, 80, and 100 times, filter with a 200-mesh sieve, collect the filtrate, and determine the indicators and dry paste rate according to the above method; the results show that the increase in the amount of water added is accompanied by a decrease in the content of each indicator and the dry paste rate, and there is no obvious trend when the amount is 80 times, so the highest amount of water is abandoned, and 40 times, 60 times, and 80 times are selected for subsequent experiments;

[0031] Step 12, choose the soaking time

[0032] Weigh 5g of the powder of Crownwood-4 Decoction, put it in a decoction pot, accurately add 60 times the amount of water, decoct once, the decoction time is 20min, the soaking time is 0min, 10min, 20min, 30min respectively, filter with a 200-mesh sieve, collect the filtrate, and determine the various indicators and dry paste rate according to the above method; the results show that the increase of soaking time increases the content of various indicators and the dry paste rate, so the minimum decoction time is abandoned, and 10min, 20min, and 30min are selected for subsequent experiments;

[0033] Step 13, orthogonal experiment

[0034] A single factor study was conducted, and decoction time (A), water addition (B), and soaking time (C) were selected as factors; the dry extract yield and the contents of gallic acid, gentiopicroside, epicatechin, dihydromyricetin, ellagic acid, and berberine were used as indicators to establish an L9 (34) orthogonal experiment. The content and dry extract yield of the test solution were determined and prepared according to the above method; the results showed that the order of influence of each factor on the comprehensive index component was A>B>C, that is, decoction time>water addition>soaking time; the order of influence on the dry extract yield was B>A>C, that is, water addition>decoction time>soaking time; each factor had no significant effect (P>0.05); considering the cost saving and extraction effectiveness, the optimal process conditions were a decoction time of 35 minutes, a water addition of 40 times, and a soaking time of 30 minutes.

[0035] Step 14: Verification test

[0036] Weigh 3 portions of medicinal materials, select the best process optimization conditions obtained in step 13 above for decoction, and measure various indicators and dry paste rate according to the above method. The results show that the content of various indicator components and dry paste rate are relatively stable, and the optimization process is reasonable and feasible.

[0037] The present invention has the following advantages:

[0038] (1) This study used high performance liquid chromatography (HPLC) and L9 (34) orthogonal experiments to determine the contents of gallic acid, epicatechin, dihydromyricetin, gentiopicroside, ellagic acid and berberine in the Mongolian medicine Sendeng-4-flavor soup, and studied the decoction and extraction process. This method is simple and rapid, and solves the scientific problem of unclear content determination of Mongolian medicine Sendeng-4-flavor soup, laying a foundation for its further content determination and quality control.

[0039] (2) The present invention uses high performance liquid chromatography to obtain six index components from the four-flavor decoction, and then classifies them with the four single herbs in the prescription. The identification results show that gallic acid and ellagic acid are derived from Terminalia chebula; epicatechin and dihydromyricetin are derived from the clary sibiricum; gentiopicroside is derived from Gentiana macrophylla; and berberine is derived from Phellodendron amurense. The above six active ingredients may become markers when determining the content of the four-flavor decoction, which will provide new development concepts for the formulation of the quality standards of the prescription or the development of innovative drugs. BRIEF DESCRIPTION OF THE DRAWINGS

[0040] Figure 1 This is a process flow chart of the decoction and extraction method of Xanthoceras sorbifolia-4-flavor soup involved in the present invention;

[0041] Figure 2 It is a comparison chart of the chromatograms of the reference substance and the sample of the present invention. DETAILED DESCRIPTION

[0042] The present invention is described in detail below in conjunction with specific embodiments. It should be noted that the following embodiments are only further descriptions of the present invention, but the protection scope of the present invention is not limited to the following embodiments.

[0043] Example

[0044] This embodiment relates to a method for decoction and extraction of Xanthoceras sorbifolia-4-flavor decoction, see Figure 1 The specific deficiencies are as follows:

[0045] 1. Instrument:

[0046] Agilent 1260 Infinity II high performance liquid chromatograph, Agilent Technologies, Inc.; SQP electronic balance (actual graduation value (d) is 0.01 mg / 0.1 mg), Sartorius Scientific Instruments (Beijing) Co., Ltd.; HH.S21-Ni4 electric constant temperature water bath, Beijing 328 Scientific Instruments Co., Ltd.; JP-040S Jiemeng ultrasonic cleaning machine, Shenzhen Jiemeng Cleaning Equipment Co., Ltd.; filter membrane (organic microporous, pore size: 0.45 μm), Tianjin Fuchengda Technology Co., Ltd.; filter membrane (water filter membrane, pore size: 0.45 μm), USA Origin Membranes.

[0047] 2. Drug testing

[0048] Gentianopicroside (110770-202219, content 98.1%), China Food and Drug Inspection Institute; Dihydromyricetin (19031501, content 99.57%), Beijing Putian Tongchuang Biotechnology Co., Ltd.; Chromatographically pure (acetonitrile, methanol), Tianjin Damao Chemical Reagent Factory; Ultrapure water, Yaner Fudi Bioanalytical Instruments (Shanghai) Co., Ltd.; Mongolian medicine Sendeng-4 flavor soup 20201120 Tongliao Horqin Left Middle Banner Mongolian Hospital (in-hospital preparation).

[0049] 3. Methods and steps:

[0050] (1) Chromatographic conditions

[0051] Venusil MPC18 chromatographic column (4.6x300 mm, 5μm); mobile phase acetonitrile (B): 0.3% phosphoric acid aqueous solution (A), gradient elution (0-6min, 2%-4% B; 6-15min, 4%-10% B; 10-59min, 10%-50% B; 59-66min, 2% B); flow rate: 1.0mL / min; column temperature: 30℃; detection wavelength: 260nm; injection volume 10μL.

[0052] (2) Preparation of reference solution

[0053] Weigh 0.79 mg of the reference substance gentiopicroside accurately and place it in a 10 mL brown volumetric bottle, 0.98 mg of gallic acid, 0.75 mg of epicatechin, 0.95 mg of dihydromyricetin, 0.66 mg of ellagic acid, and 0.61 mg of berberine in 5 mL brown volumetric bottles, add methanol to dissolve and dilute to the scale, shake well, and prepare a stock solution. Then accurately measure different amounts of each reference substance from the stock solution, add methanol to dilute to the scale, shake well, and finally obtain gentiopicroside 79 μg / mL gallic acid 19.6 μg / mL, epicatechin 24 μg / mL, dihydromyricetin 15.2 μg / mL, ellagic acid 6.325 μg / mL, berberine 73.2 μg / mL reference substance solutions.

[0054] (3) Preparation of test solution

[0055] Accurately weigh about 5g of sample powder (Mongolian medicine Scutellaria baicalensis-4-flavor soup, also known as Sendeng-4-flavor soup), put it in a decoction pot, accurately add 60 times the amount of water, decoct once, soak for 20min, decoction time is 25min, filter with a 200-mesh sieve, collect the filtrate, take another 1mL, put it in a 25mL volumetric flask, add methanol to dissolve only the scale, shake well, filter with a 0.22μm microporous filter membrane and take the filtrate.

[0056] (4) Content determination

[0057] (4.1) Linear relationship investigation

[0058] According to the reference solution prepared above, gallic acid was measured at 0.5μL, 2.5μL, 5μL, 7.5μL, 10μL, 12.5μL, and 15μL; gentiopicroside, epicatechin, and dihydromyricetin were measured at 2.5μL, 5μL, 7.5μL, and according to the linear relationship inspection reference solution, gallic acid was measured at 0.5μL, 2.5μL, 5μL, 7.5μL, 10μL, 12.5μL, and 15μL; gentiopicroside, epicatechin, and dihydromyricetin were measured at 2.5μL, 5μL, 7.5μL, 10μL, 12.5μL, and 15μL; ellagic acid and berberine were measured at 2.5μL, 5μL, 7.5μL, 10μL, 12.5μL, 15μL, 17.5μL, and 20μL. The sample volume was injected into the high performance liquid chromatograph, and the peak area was recorded. The peak area (Y) was used for regression analysis on the concentration (X). The linear relationship was good, see the linear relationship of the index components, see Figure 2 As shown in Table 1.

[0059] Table 1

[0060] Reference Regression equation Correlation coefficient r Linear range (m / μg) gallic acid Y=1419.4X+37.392 0.9996 0.0098-0.294 Gentiopicroside Y=914.81X+20.618 0.9993 0.1975-1.185 Epicatechin Y=175.78X+2.5654 0.9992 0.06-0.36 Dihydromyricetin Y=517.01X+2.5503 0.9995 0.038-0.228 Ellagic acid Y=2067.1X-1.1453 0.9998 0.0158-0.1265 Berberine Y=3251X+3.1273 0.9995 0.0076-0.061

[0061] In the above Table 1, the regression equations of the selected index components are gallic acid y=1419.4x+37.392, 0.9996, the injection amount is 0098~0.294μg; gentiopicroside y=914.81x+20.618, r=0.9993, the injection amount is 0.1975~1.185μg; epicatechin y=175.78x+2.5654, r=0.9992 0.06~0.36μg; dihydromyricetin y=517.01x+2.5503,r=0.9995; injection volume is 0.038~0.228μg; ellagic acid y=2067.1x-1.1453,r=0.99980.0158~0.1265μg; berberine y=3251x+3.1273,r=0.9995, 0.0076~0.061μg range is linear with the peak area. It can be obtained that the average recovery rates are 106.35%, 101.64%, 97.04%, 101.81%, 102.99, 98.10% respectively.

[0062] (4.2) Methodological review

[0063] (4.2.1) Precision test

[0064] Take a sample and prepare the test solution according to the above method. Accurately pipette 10 μL of the test solution under the above chromatographic conditions, inject 6 times in sequence, record the chromatographic peaks, and determine the RSD% (n=6) of the peak area, indicating that the instrument has good precision, as shown in Table 2.

[0065] (4.2.2) Stability test

[0066] Take a sample and prepare the test solution according to the above method. Accurately pipette 10 μL of the test solution under the above chromatographic conditions and inject it at 0h, 2h, 4h, 6h, 8h, 10h, 12h and 24h after preparation. Record the chromatographic peaks and determine the RSD% (n=6) of the peak area. It shows that the test solution is basically stable within 24h, as shown in Table 2.

[0067] (4.2.3) Repeatability test

[0068] Take a sample and prepare the test solution according to the above method. Accurately pipette 10 μL of the test solution under the above chromatographic conditions, record the chromatographic peak, and determine the RSD% (n=6) of the peak area, indicating that the method has good repeatability, as shown in Table 2.

[0069] Table 2

[0070] Indicator ingredients Precision (RSD%) Stability (RSD%) Repeatability (RSD%) gallic acid 1.17 1.03 1.54 Gentiopicroside 0.24 0.50 0.35 Epicatechin 1.39 1.81 1.97 Dihydromyricetin 1.45 1.70 1.77 Ellagic acid 0.77 1.66 1.27 Berberine 1.04 0.96 0.36

[0071] (4.2.4) Sample recovery test

[0072] Take 6 samples, each 2.5 g, and add gallic acid, gentiopicroside, epicatechin, dihydromyricetin, ellagic acid and berberine reference substances in a 1:1 ratio to each sample. Prepare and measure the test solution in sequence according to the test solution preparation method, and calculate the recovery rate, as shown in Table 3.

[0073] Table 3

[0074]

[0075]

[0076] (4.2.5) Sample content determination

[0077] Take a sample and prepare the test solution according to the above method. Accurately pipette 10 μL of the test solution under the above chromatographic conditions, record the peak area, calculate the content and RSD% (n=3). The results are shown in Table 4.

[0078] Table 4

[0079]

[0080]

[0081] (5) Determination of dry paste yield

[0082] 25 mL of the decoction extract is accurately measured and placed in an evaporating dish (M1) of constant mass, then dried in a drying oven at 105°C to constant weight, placed in a desiccator to cool, and accurately weighed (M2). Dry paste yield = (M2-M1) / m, where m is the total mass of the medicinal material in the sample to be tested.

[0083] (6) Single factor study

[0084] A single factor investigation was conducted using the decoction extraction method (decoction time, amount of water added, soaking time), dry paste yield, and the contents of gallic acid, gentiopicroside, epicatechin, dihydromyricetin, ellagic acid, and berberine as indicators.

[0085] (6.1) Cooking time

[0086] Weigh 5g of the powder of Crownwood-4 Decoction, put it in a decoction pot, add 60 times the amount of water, decoct once, soak for 20 minutes, and the decoction time is 5 minutes, 15 minutes, 25 minutes, and 35 minutes respectively. Filter with a 200-mesh sieve, collect the filtrate, and determine the various indicators and dry paste rate according to the above method. The results show that the increase in decoction time increases the content of various indicators and the dry paste rate, so the minimum decoction time is abandoned, and 15 minutes, 25 minutes, and 35 minutes are selected for subsequent experiments.

[0087] (6.2) Amount of water added

[0088] Weigh 5g of the powder of Crownwood-4 flavor soup, put it in a decoction pot, decoct once, soak for 20min, decoct for 35min, accurately add water at 40, 60, 80, and 100 times, filter with a 200-mesh sieve, collect the filtrate, and determine the indicators and dry paste rate according to the above method. The results show that the increase in the amount of water added reduces the content of each indicator and the dry paste rate, and there is no obvious trend when it reaches 80 times, so the highest amount of water is abandoned, and 40 times, 60 times, and 80 times are selected for subsequent experiments.

[0089] (6.3) Soaking time

[0090] Weigh 5g of the powder of the 4-flavor soup of the crown wood, put it in a decoction pot, add 60 times the amount of water, decoct once, decoction time 20min, soak time 0min, 10min, 20min, 30min, filter with a 200-mesh sieve, collect the filtrate, and determine the various indicators and dry paste rate according to the above method. The results show that the increase in soaking time increases the content of various indicators and the dry paste rate, so the minimum decoction time is abandoned, and 10min, 20min, and 30min are selected for subsequent experiments.

[0091] (7) Orthogonal experiment

[0092] The results of single factor investigation were adopted, and decoction time (A), water addition (B), and soaking time (C) were selected as the investigation factors. The dry paste yield and the contents of gallic acid, gentiopicroside, epicatechin, dihydromyricetin, ellagic acid, and berberine were used as indicators to establish the L9 (34) orthogonal experiment, as shown in Table 5. The content and dry paste rate of the test solution were determined and prepared according to the above method, as shown in Table 6 and Table 7. From the results in Table 7, it can be concluded that the order of influence of each factor on the comprehensive index component is A>B>C, that is, decoction time>water addition>soaking time; the order of influence of dry paste rate is B>A>C, that is, water addition>decoction time>soaking time; each factor has no significant effect (P>0.05). Considering the cost saving and extraction effectiveness, the optimal process conditions are selected as decoction time of 35min, water addition of 40 times, and soaking time of 30min.

[0093] Table 5

[0094] level Boiling time / min(A) Water addition / times (B) Soaking time / min(C) 1 15 40 10 2 25 60 20 3 35 80 30

[0095] Table 6

[0096]

[0097]

[0098] Note: The unit of each component in Table 6 is: (mg / g)

[0099] Table 7

[0100]

[0101] (7) Verification test

[0102] Weigh 3 portions of medicinal materials, select the best process optimization conditions for decoction, and determine the various indicators and dry paste rate according to the above method. The results show that the content of each indicator component and the dry paste rate are relatively stable, and the optimization process is reasonable and feasible, as shown in Table 8.

[0103] Table 8

[0104] Evaluation indicators 1 2 3 Gallic acid / (mg / g) 0.89 0.92 0.90 Gentiopicroside / (mg / g) 5.25 5.17 5.20 Epicatechin / (mg / g) 1.88 1.83 1.85 Dihydromyricetin / (mg / g) 0.79 0.84 0.80 Ellagic acid / (mg / g) 0.75 0.77 0.73 Berberine / (mg / g) 0.35 0.38 0.36 Dry paste yield% 15.48 15.69 15.66

[0105] The method involved in the present invention uses the extract rate and the content of each effective ingredient as indicators for content determination. Through single factor investigation and orthogonal test results, it is concluded that the optimal process conditions of the 4-flavor decoction of Senna sorbifolia are 35 minutes of decoction time, 40 times of water addition, and 30 minutes of soaking time. The decoction uses gallic acid, gentiopicroside, epicatechin, dihydromyricetin, ellagic acid and berberine as indicators; in addition, the present invention also uses high performance liquid chromatography to obtain 6 index components from the 4-flavor decoction of Senna sorbifolia respectively and attributes them to the 4 single herbs of the decoction. The identification results show that gallic acid and ellagic acid are derived from Terminalia chebula; epicatechin and dihydromyricetin are derived from Senna sorbifolia; gentiopicroside is derived from Gentiana macrophylla; and berberine is derived from Phellodendron chinense. The above 6 effective ingredients may become markers when determining the content of the Senna sorbifolia sorbifolia 4-flavor decoction, which will provide new development concepts when studying the formulation of the quality standards of the decoction or developing innovative drugs.

[0106] The above describes the specific embodiments of the present invention. It should be understood that the present invention is not limited to the above specific embodiments, and those skilled in the art may make various modifications or variations within the scope of the claims, which will not affect the essence of the present invention.

Claims

1. A decoction and extraction method of Xanthoceras sorbifolia-4-flavor decoction, characterized in that: A single factor investigation was conducted, and decoction time, water addition amount, and soaking time were selected as factors. The dry paste yield and the contents of gallic acid, gentiopicroside, epicatechin, dihydromyricetin, ellagic acid, and berberine were used as indicators to establish an L9(34) orthogonal experiment to determine and prepare the content and dry paste rate of the test solution. The optimal process conditions were obtained as follows: decoction time of 35 min, water addition amount of 40 times, and soaking time of 30 min.

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