A kind of agarwood-wood root compound volatile oil for anti-depression and its application

Abstract

The invention discloses an agarwood-wood compound volatile oil for anti-depression and its application, comprising: low-acid rapeseed oil, lemon essential oil, rosemary essential oil, peppermint essential oil, supercritical volatile agarwood essential oil, supercritical volatile wood essential oil and geranium essential oil; wherein, by volume ratio, low-acid rapeseed: lemon essential oil: rosemary essential oil: peppermint essential oil: supercritical volatile agarwood essential oil: supercritical volatile wood essential oil: geranium essential oil = 2-4: 4-6: 2-4: 4-6: 0.5-2: 0.5-2: 1-3. The agarwood-wood compound volatile oil provided by the invention plays an anti-depressant effect through atomized inhalation administration, and its mechanism of action is related to inhibiting the hyperactivity of the HPA axis and regulating the neurotransmitter level in the hippocampus, providing a natural therapy for depression patients that is administered through nasal inhalation, has a definite efficacy and is suitable for long-term use.

Description

Technical Field

[0001] The invention relates to the technical field of natural plant essential oil products, and in particular to an agarwood-wood compound volatile oil for anti-depression and an application thereof. Background Art

[0002] Depression is a common mood disorder, which is mainly manifested by persistent low mood, loss of interest in daily activities, fatigue, inattention, etc., which seriously affects the quality of life of patients. At present, the treatment of depression mainly relies on drug therapy, such as antidepressants (fluoxetine hydrochloride, etc.), but these drugs are often accompanied by certain side effects, including dependence, dizziness, gastrointestinal discomfort, insomnia, etc. In addition, the efficacy of antidepressants varies from person to person, and some patients respond poorly to drug treatment. Therefore, there is an urgent need to find alternative or auxiliary treatment methods with fewer side effects and more stable efficacy.

[0003] Aromatherapy, as an alternative therapy with a long history, has gained increasing attention in recent years. Many essential oils have the functions of regulating mood, relieving stress, improving sleep, etc. They can help relieve depression symptoms to a certain extent, and have fewer side effects than oral antidepressants.

[0004] Although single essential oils have been used to some extent in antidepressants, their therapeutic effects are often inferior to those of oral antidepressants. Studies have shown that a combination of multiple essential oil ingredients can often enhance their therapeutic effects through synergistic enhancement. However, there is currently a lack of highly effective compound volatile oil formulas specifically for depression on the market. Therefore, the development of a compound volatile oil product specifically for antidepressants has important application prospects and market demand. Summary of the invention

[0005] In order to overcome the deficiencies of the prior art, the present invention provides an agarwood-costus root compound volatile oil for anti-depression and its application, which is used to solve the technical problem that there is a lack of efficient compound volatile oil formula specifically for depression in the current market, thereby providing a natural therapy with few side effects, easy to use and effective in alleviating depression symptoms.

[0006] To solve the above problems, the technical solution adopted by the present invention is as follows:

[0007] A composite agarwood-wood essential oil for anti-depression, comprising: low-acid rapeseed oil, lemon essential oil, rosemary essential oil, peppermint essential oil, supercritical volatile agarwood essential oil, supercritical volatile wood essential oil and geranium essential oil;

[0008] Among them, calculated by volume ratio, the low-acid rapeseed: lemon essential oil: rosemary essential oil: peppermint essential oil: supercritical volatile agarwood essential oil: supercritical volatile wood fragrance essential oil: geranium essential oil = 2-4: 4-6: 2-4: 4-6: 0.5-2: 0.5-2: 1-3.

[0009] As a preferred embodiment of the present invention, the step of obtaining the supercritical volatile agarwood essential oil comprises:

[0010] The agarwood containing balsam is crushed with a grinder, and the agarwood powder is obtained after passing through a 40-60 mesh sieve, and the agarwood powder is dried at a constant temperature of 50-70°C for 5-7h;

[0011] 4-6 kg of dried agarwood powder is placed in supercritical CO 2 Extraction is carried out in an extraction device, wherein the supercritical parameters include: extraction temperature 40-50°C, extraction pressure 30-40MPa, extraction time 2.5-3.5h, supercritical CO 2 Flow rate 180-220L / h;

[0012] Place the extracted agarwood extract in an alcoholization tank and stir and alcoholize at 40-45°C for 1-2 days;

[0013] The alcoholized agarwood extract is placed in a molecular distillation device for segmented molecular distillation separation and purification to obtain the supercritical volatile agarwood essential oil.

[0014] As a preferred embodiment of the present invention, the segmented molecular distillation separation and purification comprises:

[0015] The alcoholized agarwood extract is placed in a molecular distillation device and preheated at 50-80°C;

[0016] Molecular distillation is carried out under reduced pressure conditions, the pressure is controlled at 0.01-0.05MPa, the segment temperature is 120-180℃, and volatile components with different boiling points are distilled out in sequence;

[0017] The first stage is distilled at 120-140°C and 0.04-0.05MPa to separate light volatile components;

[0018] The second stage is distillation at 140-160° C. and 0.02-0.03 MPa, and the fractions are collected to obtain the supercritical volatile agarwood essential oil;

[0019] The third stage is distilled at 160-180°C and 0.01-0.02MPa to remove the residual heavy components.

[0020] As a preferred embodiment of the present invention, the supercritical volatile agarwood essential oil collected in the second stage is measured by gas chromatography, and the content of sesquiterpenoid compounds is 40%-60% by mass percentage;

[0021] Wherein, by mass percentage, the content of borneol in the sesquiterpenoid compounds is 10%-20%, the content of linalool is 15%-25%, and the content of β-elemene is 5%-15%.

[0022] As a preferred embodiment of the present invention, the step of obtaining the supercritical volatile costus root essential oil comprises:

[0023] The root of costus root is crushed by a grinder, and after passing through a 20-30 mesh sieve, costus root powder is obtained;

[0024] Weigh 400-600g of the costusroot powder and put it into a supercritical extraction kettle, the extraction pressure is 20-30MPa, the extraction temperature is 30-40°C, the pressure of the separation kettle I is 8-10MPa, the temperature is 30-40°C, the pressure of the separation kettle II is 4-6MPa, and the temperature is 20-30°C;

[0025] The extraction time is 2-4 hours, CO 2 The flow rate is controlled at 150-200 L / h to obtain the supercritical volatile costus root essential oil.

[0026] As a preferred embodiment of the present invention, the supercritical volatile costus root essential oil is quantitatively analyzed by gas chromatography-mass spectrometry, and the content of sesquiterpenoids is 30%-50% and the content of aromatic esters is 20%-30% by mass percentage;

[0027] Among them, by mass percentage, the content of costus lactone is 5%-15%, and the content of norcostus enolone is 10%-20%.

[0028] As a preferred embodiment of the present invention, the low-acid rapeseed oil is rapeseed oil obtained by breeding or genetic modification;

[0029] Wherein, as determined by gas chromatography, the content of erucic acid in the low-acid rapeseed oil is less than 2% by mass, the content of total unsaturated fatty acids is 90%-95%, and the content of α-linolenic acid in the total unsaturated fatty acids is 5%-8%;

[0030] The lemon essential oil is extracted from fresh lemon peel by cold pressing;

[0031] Wherein, measured by gas chromatography, the content of limonene in the lemon essential oil is 60%-80% by mass percentage.

[0032] As a preferred embodiment of the present invention, the rosemary essential oil is extracted from rosemary leaves by steam distillation;

[0033] Wherein, the content of 1,8-cineole in the rosemary essential oil is 30%-50% by mass as determined by gas chromatography;

[0034] The peppermint essential oil is extracted from peppermint leaves by steam distillation;

[0035] Wherein, measured by gas chromatography, the content of menthol in the peppermint essential oil is 40%-60% by mass percentage.

[0036] As a preferred embodiment of the present invention, the geranium essential oil is extracted from geranium leaves by steam distillation;

[0037] Among them, measured by gas chromatography, in terms of mass percentage, the content of citronellol in the geranium essential oil is 35%-50%, and the content of geraniol is 15%-25%.

[0038] An application of agarwood-costusroot compound volatile oil for anti-depression, wherein the compound volatile oil is atomized and inhaled by an aromatherapy device to treat depression.

[0039] Compared with the prior art, the present invention has the following beneficial effects:

[0040] (1) The present invention provides a natural therapy with few side effects, easy to use and effective in relieving depression symptoms by optimizing the ratio and preparation process of the compound volatile oil;

[0041] (2) The agarwood-costus root compound volatile oil provided by the present invention can be inhaled by atomization to improve the gastrointestinal system, appetite and digestive function, thereby effectively preventing and relieving depression, and has fewer side effects than traditional oral antidepressants;

[0042] (3) By atomizing and sniffing the agarwood-costus root compound volatile oil provided by the present invention, the HPA axis hyperactivity caused by depression can be inhibited, thereby effectively preventing and treating depression;

[0043] (4) The agarwood-wood compound volatile oil provided by the present invention can be inhaled by atomization to significantly affect the neurotransmitters 5-HT, NE, ACh, and DA in the hippocampus, thereby reversing the decrease in neurotransmitters caused by depression.

[0044] Thus effectively preventing and treating depression;

[0045] (5) The agarwood-wood compound volatile oil provided by the present invention can be inhaled by atomization to reverse the 5-HT in the hippocampus caused by depression. 1AThe protein expression level decreases, thus effectively preventing and treating depression;

[0046] (6) The aerosol-inhalation of the agarwood-wood compound volatile oil provided by the present invention upregulated the expression of 5-HT in the hippocampus. 1A mRNA and 5-HT 1A expression, thereby effectively preventing and treating depression;

[0047] (7) Agarwood and costus root are still two commonly used aromatic Chinese medicines, but the antidepressant effect and mechanism of the combined administration of the two volatile oils by inhalation have not been studied. The study of the pharmacodynamics of agarwood-costus root compound volatile oil (CMVO) in this invention can provide a reference for other studies to explore the antidepressant effect of aromatic Chinese medicines by aerosol administration.

[0048] The present invention is further described in detail below in conjunction with the accompanying drawings and specific embodiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0049] Figure 1 Figure 2 shows the body weight of rats in each group at week 0 and week 4;

[0050] Figure 2 The effect diagram of each group of rats on baseline sucrose preference (a), final sucrose preference (b), and immobility time in FST (c);

[0051] Figure 3 Figure 2 shows the average speed (a), rest time (b), number of times entering the central area (c), total movement distance (d), and movement distance in the edge area (d) of rats in each group;

[0052] Figure 4 Heat map (f1-f6) of the movement trajectories of rats in each group;

[0053] Figure 5 The effect diagrams are ACTH level (a), CORT level (b), and CRH mRNA expression (c) of rats in each group;

[0054] Figure 6 Figure 2 shows the contents of 5-HT, NE, ACh, and DA in the hippocampus of rats in each group (a, b, c, d);

[0055] Figure 7 Western blot was used to detect 5-HT in the hippocampus of rats in each group. 1A Protein level (a, b), RT-PCR detection of 5-HT in hippocampus 1A mRNA level (c), immunohistochemical detection of 5-HT in hippocampus 1A The result display diagram of expression (d);

[0056] Figure 8These are the immunohistochemical staining images of rats in each group taken under a microscope. DETAILED DESCRIPTION

[0057] The agarwood-wood compound volatile oil for anti-depression provided by the present invention comprises: low-acid rapeseed oil, lemon essential oil, rosemary essential oil, peppermint essential oil, supercritical volatile agarwood essential oil, supercritical volatile wood essential oil and geranium essential oil;

[0058] Among them, by volume ratio, low-acid rapeseed: lemon essential oil: rosemary essential oil: peppermint essential oil: supercritical volatile agarwood essential oil: supercritical volatile wood fragrance essential oil: geranium essential oil = 2-4: 4-6: 2-4: 4-6: 0.5-2: 0.5-2: 1-3.

[0059] Preferably, calculated by volume ratio, low-acid rapeseed: lemon essential oil: rosemary essential oil: peppermint essential oil: supercritical volatile agarwood essential oil: supercritical volatile wood fragrance essential oil: geranium essential oil = 3:5:3:5:1:1:2.

[0060] Preferably, the step of obtaining supercritical volatile agarwood essential oil comprises:

[0061] The agarwood containing balsam is crushed with a grinder, and the agarwood powder is obtained after passing through a 40-60 mesh sieve, and the agarwood powder is dried at a constant temperature of 50-70°C for 5-7h;

[0062] 4-6 kg of dried agarwood powder is placed in supercritical CO 2 Extraction is carried out in an extraction device, wherein the supercritical parameters include: extraction temperature 40-50°C, extraction pressure 30-40MPa, extraction time 2.5-3.5h, supercritical CO 2 Flow rate 180-220L / h;

[0063] Place the extracted agarwood extract in an alcoholization tank and stir and alcoholize at 40-45°C for 1-2 days;

[0064] The alcoholized agarwood extract is placed in a molecular distillation device for segmented molecular distillation separation and purification to obtain supercritical volatile agarwood essential oil.

[0065] More preferably, the segmented molecular distillation separation and purification comprises:

[0066] The alcoholized agarwood extract is placed in a molecular distillation device and preheated at 50-80°C;

[0067] Molecular distillation is carried out under reduced pressure conditions, the pressure is controlled at 0.01-0.05MPa, the segment temperature is 120-180℃, and volatile components with different boiling points are distilled out in sequence;

[0068] The first stage is distilled at 120-140°C and 0.04-0.05MPa to separate light volatile components;

[0069] The second stage is distilled at 140-160°C and 0.02-0.03MPa, and the fractions are collected to obtain supercritical volatile agarwood essential oil;

[0070] The third stage is distilled at 160-180°C and 0.01-0.02MPa to remove the residual heavy components.

[0071] More preferably, the supercritical volatile agarwood essential oil collected in the second stage is determined by gas chromatography, and the content of sesquiterpenoid compounds is 40%-60% by mass percentage;

[0072] Among them, by mass percentage, the content of borneol in the sesquiterpenoid compounds is 10%-20%, the content of linalool is 15%-25%, and the content of β-elemene is 5%-15%.

[0073] Specifically, supercritical volatile agarwood essential oil can enhance the antidepressant effect of compound volatile oil by calming and regulating the nervous system.

[0074] 10%-20% borneol has a calming and anti-anxiety effect, which can enhance the antidepressant effect of the compound volatile oil. 15%-25% linalool has relaxing and calming properties, which can help relieve anxiety, depression and stress, and the aromatic properties of linalool can help improve sleep quality. In addition, linalool has a unique aroma, which can create a comfortable and peaceful environment and enhance the overall relaxation effect when used in aromatherapy. 5%-15% β-elemene can bring a relaxing and soothing effect in aromatherapy, help relieve anxiety and stress, and enhance mood.

[0075] Preferably, the step of obtaining supercritical volatile costus essential oil comprises:

[0076] The root of costus root is crushed by a grinder, and after passing through a 20-30 mesh sieve, costus root powder is obtained;

[0077] Weigh 400-600g of costusroot powder and put it into a supercritical extraction kettle, the extraction pressure is 20-30MPa, the extraction temperature is 30-40℃, the pressure of separation kettle I is 8-10MPa, the temperature is 30-40℃, the pressure of separation kettle II is 4-6MPa, the temperature is 20-30℃;

[0078] The extraction time is 2-4 hours, CO 2 The flow rate is controlled at 150-200 L / h to obtain supercritical volatile costus root essential oil.

[0079] More preferably, the supercritical volatile costus root essential oil is quantitatively analyzed by gas chromatography-mass spectrometry, and the content of sesquiterpenoids is 30%-50% and the content of aromatic esters is 20%-30% by mass percentage;

[0080] Among them, by mass percentage, the content of costus lactone is 5%-15%, the content of norcostus is 10%-20%, and the determination conditions of gas chromatography-mass spectrometry are column temperature of 150-250°C and injection volume of 0.1-0.5 μL.

[0081] More preferably, the extracted costus root essential oil is alcoholized at 40-50° C. for 1-2 days to further improve the stability and medicinal effect of the essential oil.

[0082] Specifically, supercritical costus essential oil further enhances the antidepressant effect of the compound volatile oil through its properties of soothing nerves and improving mood. Sesquiterpenoids with a content of 30%-50% have a good mood-enhancing effect, can help reduce anxiety and depression, and promote psychological relaxation. Aromatic esters with a content of 20%-30% can bring a soothing aroma and further improve mood. The interaction between sesquiterpenoids and aromatic esters can enhance the overall therapeutic effect, making the antidepressant effect of the compound volatile oil more significant.

[0083] Specifically, 5%-15% of costus lactone has a good soothing effect, helps reduce stress and anxiety, and promotes relaxation. 10%-20% of norcostus can improve mood and increase psychological comfort. Costus lactone provides a unique woody aroma, increases the aromatic depth of the compound, and enhances the user experience, while the fresh scent of norcostus can also enhance the overall fragrance effect.

[0084] More preferably, the ratio of costuslactone to norcostusene is in the range of 1:1 to 1:2.

[0085] Specifically, the above ratio can effectively balance the mild woody aroma of costus lactone with the fresh breath of norcostus, enhancing the layered sense of the overall fragrance. The two work together to enhance mood, with costus lactone providing a relaxing effect and norcostus ene enhancing the sense of freshness, which helps relieve stress and anxiety.

[0086] Preferably, the low-acid rapeseed oil is rapeseed oil obtained by breeding or genetic improvement;

[0087] Among them, determined by gas chromatography, the content of erucic acid in low-acid rapeseed oil is less than 2% by mass, the content of total unsaturated fatty acids is 90%-95%, and the content of α-linolenic acid in total unsaturated fatty acids is 5%-8%.

[0088] More preferably, the content of erucic acid in the low-acid rapeseed oil is 0.1%-1.5% by mass.

[0089] Specifically, the preparation method of low-medium acid rapeseed oil comprises the following steps:

[0090] (1) Use genetically modified or selectively bred low-erucic acid rapeseed;

[0091] (2) washing, drying and pressing the rapeseed to obtain crude oil;

[0092] (3) The crude oil is degummed, deacidified and deodorized to obtain low-acid rapeseed oil.

[0093] Specifically, low-methylene-acid rapeseed oil has high antioxidant stability, which can enhance the shelf life and stability of active ingredients of compound volatile oils. The 5%-8% α-linolenic acid has anti-inflammatory effects, which can help reduce inflammation levels in the body and support cardiovascular and brain health. In addition, α-linolenic acid also has a positive effect on improving mood and cognitive function.

[0094] Preferably, lemon essential oil is extracted from fresh lemon peels by cold pressing;

[0095] Among them, measured by gas chromatography, the content of limonene in lemon essential oil is 60%-80% by mass.

[0096] More preferably, during the extraction of lemon essential oil, the pressing temperature of the lemon peel is controlled to be no higher than 30° C. to ensure the stability of the active ingredient.

[0097] Specifically, the lemon essential oil in the present invention has the effects of anti-oxidation, refreshing and refreshing, and can enhance the antidepressant activity of the compound volatile oil. Among them, the limonene content of 60%-80% can stimulate the nervous system through the sense of smell, thereby relieving emotional disorders such as anxiety and depression, and activate the secretion of neurotransmitters (such as serotonin and dopamine), help improve mood, and play an anti-depressant role. At the same time, the smell of limonene can reduce the level of cortisol (stress hormone), thereby relieving mental stress and improving sleep quality, and assisting in regulating mood. Limonene has antioxidant properties, can neutralize free radicals in the body, reduce the damage of oxidative stress to the nervous system, and then protect the brain and nerve function, and support the antidepressant effect. Limonene can promote the human absorption and bioavailability of other components (such as sesquiterpenes and costus lactone) in the compound volatile oil, improve the overall efficacy, and can play a synergistic role to enhance the antidepressant effect of multiple active ingredients in the compound.

[0098] Preferably, rosemary essential oil is extracted from rosemary leaves by steam distillation;

[0099] Among them, measured by gas chromatography, the content of 1,8-cineole in rosemary essential oil is 30%-50% by mass.

[0100] More preferably, the distillation temperature during the extraction of rosemary essential oil is controlled below 100° C. to ensure the stability of its volatile components.

[0101] Specifically, rosemary essential oil has the effects of promoting blood circulation, relieving mental stress, and enhancing the antidepressant effect of compound volatile oil. Among them, 1,8-cineole with a content of 30%-50% has significant neuroprotective and mood regulating effects, which can help relieve anxiety and depression symptoms. At the same time, 1,8-cineole has strong anti-inflammatory and antioxidant properties, which can neutralize free radicals in the body and reduce the damage of oxidative stress to the nervous system, which is crucial for anti-depression and protecting brain function. In addition, 1,8-cineole, as an ingredient with a fresh aroma, not only enhances the fragrance experience of the compound, but also produces a relaxing and refreshing effect when used, enhancing the pleasure of aromatherapy. In combination with other essential oil ingredients, 1,8-cineole can play a synergistic role, enhance the efficacy of other ingredients in the compound, and enhance the overall biological activity.

[0102] Preferably, peppermint essential oil is extracted from peppermint leaves by steam distillation;

[0103] Among them, measured by gas chromatography, the content of menthol in peppermint essential oil is 40%-60% by mass.

[0104] More preferably, during the extraction of peppermint essential oil, the distillation temperature is controlled below 90° C. to ensure the retention of the active ingredient menthol.

[0105] Specifically, peppermint essential oil has a refreshing and refreshing effect, can relieve anxiety and depression, and enhance the antidepressant effect of compound volatile oil. Among them, menthol with a content of 40%-60% has obvious sedative and relaxing effects, and provides a soothing and cooling feeling by activating the cold receptors in the body, which helps to relieve tension and anxiety and help regulate emotions. The refreshing smell of menthol can quickly refresh the mind, promote concentration and mental clarity, reduce fatigue, and help improve cognitive ability, which has a positive effect on anti-depressive symptoms, can enhance emotions and promote a positive psychological state. Menthol is used to relieve headaches and migraines in aromatherapy, and it can reduce head pressure and relieve pain through the effects of cold sensation and local vasoconstriction. This has a special auxiliary effect for people who are in a state of depression and tension for a long time. Menthol can soothe the respiratory tract, help improve nasal congestion and dyspnea, and is particularly suitable for the relief of respiratory problems such as colds or allergies. The menthol in the compound volatile oil can effectively relieve anxiety or discomfort caused by poor breathing. The cooling sensation brought by menthol not only provides a comfortable experience physically, but also brings a relaxing and refreshing feeling mentally, helping to relieve irritability caused by stress or fatigue.

[0106] Preferably, geranium essential oil is extracted from geranium leaves by steam distillation;

[0107] Among them, determined by gas chromatography, the content of citronellol in geranium essential oil is 35%-50% and the content of geraniol is 15%-25% by mass.

[0108] More preferably, the extraction temperature of geranium essential oil is controlled below 95°C to ensure the stability and efficacy of its volatile components. The purity of geranium essential oil is above 90% without any chemical additives to ensure its naturalness and safety.

[0109] Specifically, geranium essential oil has the function of regulating emotions and balancing hormones, and can enhance the antidepressant effect of compound volatile oils.

[0110] Geranium essential oil with a content of 35%-50% and geraniol with a content of 15%-25% both have significant mood regulation effects. Citronellol can help relieve anxiety and depression symptoms, promote psychological relaxation, and improve mood. Geraniol also has a calming effect and helps improve anxiety. Citronellol and geraniol in geranium essential oil add a fresh and herbal aroma to the compound volatile oil, which can enhance the user experience, promote psychological relaxation, and enhance the overall aromatherapy effect. Citronellol and geraniol can produce a mild soothing feeling during use, helping to relieve tension caused by stress or physical discomfort. In the compound volatile oil, these two ingredients can cooperate with each other to exert a synergistic effect, enhance the overall efficacy, and make the compound volatile oil more effective in anti-depression.

[0111] The agarwood-costus root compound volatile oil, which is used for anti-depression, is nebulized and inhaled through an aromatherapy device to treat depression.

[0112] The following examples are provided to further illustrate the present invention, but the scope of the present invention is not limited thereto.

[0113] Agarwood has been a precious aromatic Chinese medicine since ancient times, with the effects of promoting qi and relieving pain, warming the middle and stopping vomiting. Modern pharmacological studies have shown that the volatile oil in agarwood is its main active ingredient, which has good pharmacological effects on the central nervous system, and has sedative, anti-depressant and neuroprotective effects. Costus root has the effects of promoting qi and relieving pain, strengthening the spleen and digestion, and the volatile oil of costus root is also its main pharmacological substance, which has potential neuroprotective effects. In addition, the most important pharmacological activity of costus root is that it has a good therapeutic effect on gastrointestinal diseases. Although agarwood and costus root are two commonly used aromatic Chinese medicines, the antidepressant effect and mechanism of the two volatile oils combined by inhalation have not been studied yet.

[0114] Liver depression and spleen deficiency are the main clinical syndromes of depression. Modern studies have shown that depression may be closely related to gastrointestinal function. On the one hand, among the complications of depression, gastrointestinal dysfunction is the most common. Most patients experience symptoms such as indigestion, nausea and vomiting, and loss of appetite. 36-63% of patients with depression will have gastrointestinal symptoms. On the other hand, the occurrence of gastrointestinal diseases such as functional dyspepsia can also cause patients to further develop anxiety or depression. Studies have found that the incidence of depression in patients with gastrointestinal diseases is five times that of non-diseased people.

[0115] In this example, agarwood and costus root are used as the main drugs to prepare agarwood compound volatile oil. The aromatic aroma of agarwood can promote qi and relieve depression, and costus root can strengthen the spleen and digestion, promote qi and relieve pain. From the perspective of nasal administration of aromatic Chinese medicine to prevent and treat depression, the antidepressant effect and pharmacodynamic mechanism of the aromatic Chinese medicine on the rat model of depression induced by chronic unpredictable mild stress are explored.

[0116] In the following examples, the Agarwood-Aucklandia compound volatile oil is diluted with Meadowfoam seed oil and then administered by atomization and inhalation through an aromatherapy device.

[0117] Embodiment 1

[0118] The dilution ratio is Meadowfoam seed oil: Agarwood-Saussurea compound volatile oil = 30:1 (volume ratio).

[0119] By volume ratio, the low-acid rapeseed essential oil in the agarwood-wood compound volatile oil: lemon essential oil: rosemary essential oil: peppermint essential oil: supercritical volatile agarwood essential oil: supercritical volatile wood essential oil: geranium essential oil = 2:4:2:4:0.5:0.5:1.

[0120] The steps for obtaining supercritical volatile agarwood essential oil include:

[0121] The agarwood containing balsam was crushed with a grinder, and the agarwood powder was obtained after passing through a 40-mesh sieve, and the agarwood powder was dried at a constant temperature of 50°C for 5 hours;

[0122] 4 kg of dried agarwood powder was placed in supercritical CO 2 The extraction was carried out in an extraction device, wherein the supercritical parameters included: extraction temperature 40°C, extraction pressure 30MPa, extraction time 2.5h, supercritical CO 2 Flow rate: 180L / h;

[0123] The extracted agarwood extract was placed in an alcoholization tank and stirred for alcoholization at 40°C for 1 day;

[0124] The alcoholized agarwood extract is placed in a molecular distillation device for segmented molecular distillation separation and purification to obtain supercritical volatile agarwood essential oil.

[0125] The segmented molecular distillation separation and purification includes:

[0126] The alcoholized agarwood extract was placed in a molecular distillation device and preheated at 50°C;

[0127] Molecular distillation was carried out under reduced pressure, the pressure was controlled at 0.01MPa, the segment temperature was 120℃, and volatile components with different boiling points were distilled out in sequence;

[0128] The first stage is distilled at 120°C and 0.04MPa to separate the light volatile components;

[0129] The second stage is distilled at 140°C and 0.02MPa, and the fractions are collected to obtain supercritical volatile agarwood essential oil;

[0130] The third stage is distilled at 160°C and 0.01MPa to remove residual heavy components.

[0131] The supercritical volatile agarwood essential oil collected in the second stage was determined by gas chromatography, and the content of sesquiterpenoid compounds was 40%-60% by mass percentage;

[0132] Among them, by mass percentage, the content of borneol in the sesquiterpenoid compounds is 10%-20%, the content of linalool is 15%-25%, and the content of β-elemene is 5%-15%.

[0133] The steps for obtaining supercritical volatile wood fragrance essential oil include:

[0134] The root of costus root is crushed by a grinder, and passed through a 20-mesh sieve to obtain costus root powder;

[0135] Weigh 400g of costusroot powder and put it into a supercritical extraction kettle, the extraction pressure is 20MPa, the extraction temperature is 30℃, the pressure of separation kettle I is 8MPa, the temperature is 30℃, the pressure of separation kettle II is 4MPa, the temperature is 20℃;

[0136] The extraction time was 2 hours, CO 2 The flow rate is controlled at 150L / h to obtain supercritical volatile costus root essential oil.

[0137] Supercritical volatile costus root essential oil, quantitatively analyzed by gas chromatography-mass spectrometry, showed that the content of sesquiterpenoids was 30%-50% and the content of aromatic esters was 20%-30% by mass percentage;

[0138] Among them, by mass percentage, the content of costus lactone is 5%-15%, and the content of norcostus enolone is 10%-20%.

[0139] Low-medium acid rapeseed oil is rapeseed oil obtained through breeding or genetic improvement. Determined by gas chromatography, the erucic acid content is less than 2% by mass, the content of total unsaturated fatty acids is 90%-95%, and the content of α-linolenic acid in the total unsaturated fatty acids is 5%-8%.

[0140] Lemon essential oil is extracted from fresh lemon peel by cold pressing. The content of limonene in lemon essential oil is 60%-80% by mass as determined by gas chromatography.

[0141] Rosemary essential oil is extracted from rosemary leaves by steam distillation. Gas chromatography is used to determine that, by mass percentage, the content of 1,8-cineole in the rosemary essential oil is 30%-50%.

[0142] Peppermint essential oil is extracted from peppermint leaves by steam distillation. The content of menthol in the peppermint essential oil is 40%-60% by mass as determined by gas chromatography.

[0143] Geranium essential oil is extracted from geranium leaves by steam distillation and determined by gas chromatography. The content of citronellol in geranium essential oil is 35%-50% and the content of geraniol is 15%-25% by mass.

[0144] Embodiment 2

[0145] The dilution ratio is Meadowfoam seed oil: Agarwood-Saussurea compound volatile oil = 35:1 (volume ratio).

[0146] By volume ratio, the low-acid rapeseed essential oil in the agarwood-wood compound volatile oil: lemon essential oil: rosemary essential oil: peppermint essential oil: supercritical volatile agarwood essential oil: supercritical volatile wood essential oil: geranium essential oil = 3:5:3:5:1:1:2.

[0147] The steps for obtaining supercritical volatile agarwood essential oil include:

[0148] The agarwood containing balsam was crushed with a grinder, and the agarwood powder was obtained after passing through a 50-mesh sieve, and the agarwood powder was dried at a constant temperature of 60°C for 6 hours;

[0149] 5 kg of dried agarwood powder was placed in supercritical CO 2 The extraction was carried out in an extraction device, wherein the supercritical parameters included: extraction temperature 45°C, extraction pressure 35MPa, extraction time 3h, supercritical CO 2 Flow rate 200L / h;

[0150] The extracted agarwood extract was placed in an alcoholization tank and stirred for alcoholization at 42°C for 1.5 days;

[0151] The alcoholized agarwood extract is placed in a molecular distillation device for segmented molecular distillation separation and purification to obtain supercritical volatile agarwood essential oil.

[0152] The segmented molecular distillation separation and purification includes:

[0153] The alcoholized agarwood extract was placed in a molecular distillation device and preheated at 65°C;

[0154] Molecular distillation was carried out under reduced pressure, the pressure was controlled at 0.03MPa, the segment temperature was 150°C, and volatile components with different boiling points were distilled out in sequence;

[0155] The first stage is distilled at 130°C and 0.04MPa to separate the light volatile components;

[0156] The second stage is distilled at 150°C and 0.02MPa, and the fractions are collected to obtain supercritical volatile agarwood essential oil;

[0157] The third stage is distilled at 170°C and 0.01MPa to remove residual heavy components.

[0158] The supercritical volatile agarwood essential oil collected in the second stage was determined by gas chromatography, and the content of sesquiterpenoid compounds was 40%-60% by mass percentage;

[0159] Among them, by mass percentage, the content of borneol in the sesquiterpenoid compounds is 10%-20%, the content of linalool is 15%-25%, and the content of β-elemene is 5%-15%.

[0160] The steps for obtaining supercritical volatile wood fragrance essential oil include:

[0161] The root of costus root is crushed by a grinder, and passed through a 20-mesh sieve to obtain costus root powder;

[0162] Weigh 500g of costusroot powder and put it into a supercritical extraction kettle, the extraction pressure is 25MPa, the extraction temperature is 35℃, the pressure of separation kettle I is 9MPa, the temperature is 35℃, the pressure of separation kettle II is 5MPa, the temperature is 25℃;

[0163] The extraction time was 3 hours, CO 2 The flow rate is controlled at 175 L / h to obtain supercritical volatile costus root essential oil.

[0164] Supercritical volatile costus root essential oil, quantitatively analyzed by gas chromatography-mass spectrometry, showed that the content of sesquiterpenoids was 30%-50% and the content of aromatic esters was 20%-30% by mass percentage;

[0165] Among them, by mass percentage, the content of costus lactone is 5%-15%, and the content of norcostus enolone is 10%-20%.

[0166] Low-medium acid rapeseed oil is rapeseed oil obtained through breeding or genetic improvement. Determined by gas chromatography, the erucic acid content is less than 2% by mass, the content of total unsaturated fatty acids is 90%-95%, and the content of α-linolenic acid in the total unsaturated fatty acids is 5%-8%.

[0167] Lemon essential oil is extracted from fresh lemon peel by cold pressing. The content of limonene in lemon essential oil is 60%-80% by mass as determined by gas chromatography.

[0168] Rosemary essential oil is extracted from rosemary leaves by steam distillation. Gas chromatography is used to determine that, by mass percentage, the content of 1,8-cineole in the rosemary essential oil is 30%-50%.

[0169] Peppermint essential oil is extracted from peppermint leaves by steam distillation. The content of menthol in the peppermint essential oil is 40%-60% by mass as determined by gas chromatography.

[0170] Geranium essential oil is extracted from geranium leaves by steam distillation and determined by gas chromatography. The content of citronellol in geranium essential oil is 35%-50% and the content of geraniol is 15%-25% by mass.

[0171] Embodiment 3

[0172] The dilution ratio is Meadowfoam seed oil: Agarwood-Saussurea compound volatile oil = 40:1 (volume ratio).

[0173] By volume ratio, the low-acid rapeseed essential oil in the agarwood-wood compound volatile oil: lemon essential oil: rosemary essential oil: peppermint essential oil: supercritical volatile agarwood essential oil: supercritical volatile wood fragrance essential oil: geranium essential oil = 4:6:4:6:2:2:3.

[0174] The steps for obtaining supercritical volatile agarwood essential oil include:

[0175] The agarwood containing balsam was crushed with a grinder, and the agarwood powder was obtained after passing through a 60-mesh sieve, and the agarwood powder was dried at a constant temperature of 70°C for 7 hours;

[0176] 6 kg of dried agarwood powder was placed in supercritical CO 2 The extraction was carried out in an extraction device, wherein the supercritical parameters included: extraction temperature 50°C, extraction pressure 40MPa, extraction time 3h, supercritical CO 2 Flow rate: 220L / h;

[0177] The extracted agarwood extract was placed in an alcoholization tank and stirred for alcoholization at 45°C for 2 days;

[0178] The alcoholized agarwood extract is placed in a molecular distillation device for segmented molecular distillation separation and purification to obtain supercritical volatile agarwood essential oil.

[0179] The segmented molecular distillation separation and purification includes:

[0180] The alcoholized agarwood extract was placed in a molecular distillation device and preheated at 80°C;

[0181] Molecular distillation was carried out under reduced pressure, the pressure was controlled at 0.05MPa, the segment temperature was 180℃, and volatile components with different boiling points were distilled out in sequence;

[0182] The first stage is distilled at 140°C and 0.05MPa to separate the light volatile components;

[0183] The second stage is distilled at 160°C and 0.03MPa, and the fractions are collected to obtain supercritical volatile agarwood essential oil;

[0184] The third stage is distilled at 180°C and 0.02MPa to remove residual heavy components.

[0185] The supercritical volatile agarwood essential oil collected in the second stage was determined by gas chromatography, and the content of sesquiterpenoid compounds was 40%-60% by mass percentage;

[0186] Among them, by mass percentage, the content of borneol in the sesquiterpenoid compounds is 10%-20%, the content of linalool is 15%-25%, and the content of β-elemene is 5%-15%.

[0187] The steps for obtaining supercritical volatile wood fragrance essential oil include:

[0188] The root of costus root is crushed by a grinder, and the powder is obtained after passing through a 30-mesh sieve;

[0189] Weigh 600g of costusroot powder and put it into a supercritical extraction kettle, the extraction pressure is 30MPa, the extraction temperature is 40℃, the pressure of separation kettle I is 10MPa, the temperature is 40℃, the pressure of separation kettle II is 6MPa, the temperature is 30℃;

[0190] The extraction time was 4 hours, CO 2 The flow rate is controlled at 200 L / h to obtain supercritical volatile costus root essential oil.

[0191] Supercritical volatile costus root essential oil, quantitatively analyzed by gas chromatography-mass spectrometry, showed that the content of sesquiterpenoids was 30%-50% and the content of aromatic esters was 20%-30% by mass percentage;

[0192] Among them, by mass percentage, the content of costus lactone is 5%-15%, and the content of norcostus enolone is 10%-20%.

[0193] Low-medium acid rapeseed oil is rapeseed oil obtained through breeding or genetic improvement. Determined by gas chromatography, the erucic acid content is less than 2% by mass, the content of total unsaturated fatty acids is 90%-95%, and the content of α-linolenic acid in the total unsaturated fatty acids is 5%-8%.

[0194] Lemon essential oil is extracted from fresh lemon peel by cold pressing. The content of limonene in lemon essential oil is 60%-80% by mass as determined by gas chromatography.

[0195] Rosemary essential oil is extracted from rosemary leaves by steam distillation. Gas chromatography is used to determine that, by mass percentage, the content of 1,8-cineole in the rosemary essential oil is 30%-50%.

[0196] Peppermint essential oil is extracted from peppermint leaves by steam distillation. The content of menthol in the peppermint essential oil is 40%-60% by mass as determined by gas chromatography.

[0197] Geranium essential oil is extracted from geranium leaves by steam distillation and determined by gas chromatography. The content of citronellol in geranium essential oil is 35%-50% and the content of geraniol is 15%-25% by mass.

[0198] Effects of sniffing CMVO on depressive-like behavior in CUMS rats

[0199] OFT, FST and SPT (sugar preference) were used to detect the effect of CMVO on the depressive-like behavior of CUMS rats, and the body weight, food intake and other information of all rats during the modeling and drug administration process were recorded.

[0200] (1) Effect of CMVO inhalation on body weight of rats

[0201] Before modeling, there was no significant difference in the baseline body weight among the groups. Figure 1 After 4 weeks of treatment, the body weight differences among the groups were statistically significant ( Compared with the blank group (modeling without chronic stress stimulation), the body weight of rats in the other groups (modeling after chronic stress stimulation) decreased significantly compared with the blank group ( ), wherein the other groups include: control group (no administration), fluoxetine hydrochloride group (oral administration of fluoxetine hydrochloride), Example 1 group (nebulized inhalation administration of the compound volatile oil of Example 1), Example 2 group (nebulized inhalation administration of the compound volatile oil of Example 2) and Example 3 group (nebulized inhalation administration of the compound volatile oil of Example 3). Therefore, the modeling of chronic stress stimulation affects the gastrointestinal system of rats, and affects their appetite, digestive function, and individual development.

[0202] Depend on Figure 1 It can be seen that compared with the control group, the body weight of the rats in Example 1, Example 2 and Example 3 groups increased significantly ( or ). It can be seen that the inhalation of the agarwood-wood compound volatile oil (CMVO) of Examples 1-3 can effectively reduce the impact of chronic stress stimulation on the gastrointestinal system of rats, and reduce the impact on their appetite, digestive function, and individual development.

[0203] However, compared with the control group, the body weight of rats in the fluoxetine hydrochloride group was significantly decreased ( ), indicating that oral administration of fluoxetine hydrochloride is accompanied by certain side effects, which further affect the gastrointestinal system of rats.

[0204] (2) Effect of sniffing CMVO on rats’ preference for sugar water

[0205] Before modeling, there was no significant difference in baseline sucrose preference among the groups. Figure 2 (a) is shown. After the experiment, the results of the sucrose preference test are shown in Figure 2 (b) The results showed that there were significant differences in sucrose preference among the different groups ( ). Compared with the blank group, the sucrose preference rate of rats in the control group was significantly reduced ( ). Compared with the control group, the sucrose preference rates of the fluoxetine hydrochloride group, Example 1 group, Example 2 group, and Example 3 group were all increased, and there were significant differences between the fluoxetine hydrochloride group, Example 2 group, and Example 3 group and the control group ( ).

[0206] It can be seen that whether taking fluoxetine hydrochloride orally or inhaling the agarwood-costus root compound volatile oil (CMVO) of Examples 1-3, it can effectively reduce the effect of chronic stress stimulation modeling on the sucrose preference rate of rats.

[0207] (3) Effects of sniffing CMVO on the immobility time in the forced swim test (FST) of rats

[0208] The experimental test results of immobility time in FST are shown in Figure 2 (c) There was a statistically significant difference in the immobility time between the two groups ( The immobility time of the rats in the control group in the 5-min test was significantly longer than that in the blank group ( After treatment, the immobility time of rats in the fluoxetine hydrochloride group and each example group was shortened, and there were extremely significant differences between the fluoxetine hydrochloride group, Example 1 group, and Example 3 group and the control group ( ), there is a significant difference between the Example 2 group and the control group ( ).

[0209] It can be seen that whether taking fluoxetine hydrochloride orally or inhaling the agarwood-costus root compound volatile oil (CMVO) of Examples 1-3, it can effectively reduce the effect of chronic stress stimulation modeling on the immobility time in the forced swimming test (FST) of rats.

[0210] (4) Effects of sniffing CMVO on open field test (OFT) parameters in rats

[0211] Figure 3 (ae) shows the effect of CMVO sniffing administration on the behavior of CUMS rats in OFT. The results of one-way ANOVA showed that there were significant differences in the mean speed (V.Mean), moving distance (Total dist), rest time (Res.T (s)), number of entries into the central zone (N.Ent in central zone (times)), and distance moved in the marginal zone (Dist in the marginal zone (cm)) among the groups. ; ; ; ; ).

[0212] Compared with the blank group, the average speed, moving distance, number of times entering the central area, and distance moved in the edge area of ​​the control group rats were significantly reduced ( or ), rest time increased significantly ( ), indicating that the control rats showed reduced activity and curiosity towards the new environment.

[0213] Compared with the control group, the average speed and moving distance of rats in the fluoxetine hydrochloride group and each embodiment group were significantly increased ( The rest time of rats in each embodiment group was significantly reduced ( ); The number of times the rats in Example 2 and Example 3 groups entered the central area increased significantly ( The distance moved by rats in the marginal area in each embodiment group was significantly increased ( ).

[0214] It can be seen that the rats that sniffed the agarwood-costus root compound volatile oil (CMVO) of Examples 1-3 had a stronger desire to explore the environment, and the CMVO of Examples 1-3 showed a certain antidepressant effect.

[0215] Figure 4 The heat map of the movement trajectory of rats in the open field within 5 minutes is shown, wherein f(1) corresponds to the blank group, f(2) corresponds to the control group, f(3) corresponds to the fluoxetine hydrochloride group, f(4) corresponds to the Example 1 group, f(5) corresponds to the Example 2 group, and f(6) corresponds to the Example 3 group.

[0216] Depend on Figure 4 It can be seen intuitively that the rats in each embodiment group are significantly more active than those in the control group, and have a significantly stronger willingness to explore the environment.

[0217] (5) Effects of CMVO inhalation on the activity of the hypothalamus-pituitary-adrenal (HPA) axis in rats

[0218] To study the regulatory effect of CMVO on the HPA axis of CUMS rats, the levels of serum ACTH and CORT were detected by ELISA, and the expression of hypothalamic CRH mRNA was detected by RT-PCR. There were significant differences in serum ACTH and CORT levels and hypothalamic CRH mRNA expression among the groups ( ; ; ).

[0219] The levels of serum ACTH and CORT in the control group were significantly higher than those in the blank group ( ). Compared with the control group, the ACTH levels in the fluoxetine hydrochloride group and each example group were significantly reduced, and there was a significant difference between the fluoxetine hydrochloride group and the example 1 group and the control group ( The fluoxetine hydrochloride group and the example groups were also able to reverse the increase in serum CORT caused by modeling, and there were significant differences compared with the control group ( or ).

[0220] RT-PCR results showed that the expression of CRH mRNA in the hypothalamus of rats in the control group was significantly increased ( ), the CRH mRNA expression in the fluoxetine hydrochloride group and each embodiment group was significantly lower than that in the control group ( ), the results are as follows Figure 5 (ac) This result indicates that the modeling of chronic stress stimulation leads to hyperactivity of the HPA axis in rats, increased levels of ACTH and CORT in serum, and increased expression of CRH mRNA in the hypothalamus. Inhaling the agarwood-wood compound volatile oil (CMVO) of Examples 1-3 can inhibit the hyperactivity of the HPA axis, which is one of the main mechanisms of the agarwood-wood compound volatile oil (CMVO) provided by the present invention for treating depression.

[0221] (6) Effects of CMVO inhalation on neurotransmitter levels in rat hippocampus

[0222] By detecting the neurotransmitter levels in the hippocampus, one of the mechanisms of CMVO in preventing and treating depression was revealed, which showed that it had a significant effect on the neurotransmitters 5-HT, NE, ACh, and DA in the hippocampus ( ; ; ; The levels of 5-HT, NE, ACh, and DA in the hippocampus of the control group were significantly lower than those in the blank group ( or ),like Figure 6 As shown in Table 1. CMVO administration can reverse the decrease of neurotransmitters caused by chronic stress stimulation modeling. Compared with the control group, the 5-HT levels in the fluoxetine hydrochloride group and each example group were significantly increased ( or ). Compared with the control group, the NE content in the fluoxetine hydrochloride group and each embodiment group increased to varying degrees, and there were significant differences between the fluoxetine hydrochloride group and each embodiment group and the control group ( or After treatment with fluoxetine hydrochloride and CMVO, the ACh level was significantly higher than that of the control group, and there were significant differences between the embodiment groups and the control group ( or ). Compared with the control group, the DA content in the fluoxetine hydrochloride group and each embodiment group was significantly increased ( ).

[0223] Table 1 5-HT, NE, ACh, and DA contents in hippocampus ( )

[0224]

[0225] (7) Effect of CMVO inhalation on 5-HT in rat hippocampus 1A Effect of protein expression level

[0226] By calculating 5-HT 1A The ratio of the gray value of the β-actin band to the gray value of the β-actin band was used for semi-quantitative analysis of 5-HT in the rat hippocampus. 1A The results showed that compared with the blank group, the expression of 5-HT in the hippocampus of the control group rats 1A The relative expression of the protein was significantly decreased ( ),like Figure 7 (a) and Figure 7 Compared with the control group, the 5-HT 1A The protein expression was significantly increased ( ), compared with the control group, the Example 1 group and the Example 3 group increased significantly ( ).

[0227] It can be seen that inhaling the agarwood-wood compound volatile oil (CMVO) of Examples 1-3 can reverse the 5-HT in the rat hippocampus caused by the modeling of chronic stress stimulation. 1A Decrease in protein expression levels.

[0228] (8) Effect of CMVO on 5-HT in rat hippocampus 1A Effects of mRNA expression

[0229] Similarly, RT-PCR analysis results showed that compared with the blank group, the 5-HT 1A The expression of mRNA was significantly decreased ( ), while 5-HT 1A The expression of mRNA was significantly increased compared with the control group ( ),like Figure 7 (c) shows that after sniffing the agarwood-wood compound volatile oil (CMVO) of Example 1-3, 5-HT was upregulated. 1A Expression of mRNA.

[0230] (9) Effect of CMVO inhalation on 5-HT in rat hippocampus 1A The impact of expression

[0231] Immunohistochemical method was used to detect the expression of 5-HT in the hippocampus of rats in each group 1A Positive cells, such as Figure 7 (d) As shown. Immunohistochemical staining pictures were taken under a microscope, such as Figure 8(a) (magnification ×200) and Figure 8 (b) (magnification ×400). Analysis of 5-HT in the hippocampus 1A Compared with the blank group, the OD value of positive cells in the control group was 5-HT 1A The expression of positive cells was significantly reduced ( ). Compared with the control group, the 5-HT 1A The expression of positive cells increased significantly ( or ).

[0232] Summarize

[0233] The above examples confirm that the agarwood-costus root compound volatile oil provided by the present invention has an antidepressant effect on CUMS rats through aerosol inhalation administration, and its mechanism of action is related to inhibiting the hyperactivity of the HPA axis and regulating the level of neurotransmitters in the hippocampus, providing a natural therapy for patients with depression that can be administered through nasal inhalation, has a definite therapeutic effect, and is suitable for long-term use.

[0234] The above-mentioned embodiments are only preferred embodiments of the present invention and cannot be used to limit the scope of protection of the present invention. Any non-substantial changes and substitutions made by technicians in this field on the basis of the present invention shall fall within the scope of protection required by the present invention.

Claims

1. A compound volatile oil of agarwood and costusroot for anti-depression, characterized in that: include: Low-acid rapeseed oil, lemon essential oil, rosemary essential oil, peppermint essential oil, supercritical volatile agarwood essential oil, supercritical volatile wood fragrance essential oil and geranium essential oil; Wherein, by volume ratio, the low-acid rapeseed: lemon essential oil: rosemary essential oil: peppermint essential oil: supercritical volatile agarwood essential oil: supercritical volatile wood fragrance essential oil: geranium essential oil = 2-4: 4-6: 2-4: 4-6: 0.5-2: 0.5-2: 1-3; The step of obtaining the supercritical volatile agarwood essential oil comprises: The agarwood containing balsam is crushed with a grinder, and the agarwood powder is obtained after passing through a 40-60 mesh sieve, and the agarwood powder is dried at a constant temperature of 50-70°C for 5-7h; 4-6 kg of dried agarwood powder is placed in a supercritical CO2 extraction device for extraction, wherein the supercritical parameters include: extraction temperature 40-50°C, extraction pressure 30-40MPa, extraction time 2.5-3.5h, supercritical CO2 flow rate 180-220L / h; Place the extracted agarwood extract in an alcoholization tank and stir and alcoholize at 40-45°C for 1-2 days; The alcoholized agarwood extract is placed in a molecular distillation device for segmented molecular distillation separation and purification to obtain the supercritical volatile agarwood essential oil.

2. The agarwood-wood compound volatile oil for anti-depression according to claim 1, characterized in that: The segmented molecular distillation separation and purification comprises: The alcoholized agarwood extract is placed in a molecular distillation device and preheated at 50-80°C; Molecular distillation is carried out under reduced pressure conditions, the pressure is controlled at 0.01-0.05MPa, the segment temperature is 120-180℃, and volatile components with different boiling points are distilled out in sequence; The first stage is distilled at 120-140°C and 0.04-0.05MPa to separate light volatile components; The second stage is distillation at 140-160° C. and 0.02-0.03 MPa, and the fractions are collected to obtain the supercritical volatile agarwood essential oil; The third stage is distilled at 160-180°C and 0.01-0.02MPa to remove the residual heavy components.

3. The agarwood-wood compound volatile oil for anti-depression according to claim 2, characterized in that: The supercritical volatile agarwood essential oil collected in the second stage is measured by gas chromatography, and the content of sesquiterpenoid compounds is 40%-60% by mass percentage; Wherein, by mass percentage, the content of borneol in the sesquiterpenoid compounds is 10%-20%, the content of linalool is 15%-25%, and the content of β-elemene is 5%-15%.

4. The agarwood-wood compound volatile oil for anti-depression according to any one of claims 1 to 3, characterized in that: The step of obtaining the supercritical volatile costus root essential oil comprises: The root of costus root is crushed by a grinder, and after passing through a 20-30 mesh sieve, costus root powder is obtained; Weigh 400-600g of the costusroot powder and put it into a supercritical extraction kettle, the extraction pressure is 20-30MPa, the extraction temperature is 30-40°C, the pressure of the separation kettle I is 8-10MPa, the temperature is 30-40°C, the pressure of the separation kettle II is 4-6MPa, and the temperature is 20-30°C; The extraction time is 2-4 hours, and the flow rate of CO2 is controlled at 150-200 L / h to obtain the supercritical volatile costus root essential oil.

5. The agarwood-wood compound volatile oil for anti-depression according to claim 4, characterized in that: The supercritical volatile costus root essential oil is quantitatively analyzed by gas chromatography-mass spectrometry, and the content of sesquiterpenoids is 30%-50% and the content of aromatic esters is 20%-30% by mass percentage; Among them, by mass percentage, the content of costus lactone is 5%-15%, and the content of norcostus enolone is 10%-20%.

6. The agarwood-wood compound volatile oil for anti-depression according to claim 5, characterized in that: The low-acid rapeseed oil is rapeseed oil obtained through breeding or genetic modification; Wherein, as determined by gas chromatography, the content of erucic acid in the low-acid rapeseed oil is less than 2% by mass, the content of total unsaturated fatty acids is 90%-95%, and the content of α-linolenic acid in the total unsaturated fatty acids is 5%-8%; The lemon essential oil is extracted from fresh lemon peel by cold pressing; Wherein, measured by gas chromatography, the content of limonene in the lemon essential oil is 60%-80% by mass percentage.

7. The agarwood-wood compound volatile oil for anti-depression according to claim 6, characterized in that: The rosemary essential oil is extracted from rosemary leaves by steam distillation; Wherein, the content of 1,8-cineole in the rosemary essential oil is 30%-50% by mass as determined by gas chromatography; The peppermint essential oil is extracted from peppermint leaves by steam distillation; Wherein, measured by gas chromatography, the content of menthol in the peppermint essential oil is 40%-60% by mass percentage.

8. The agarwood-wood compound volatile oil for anti-depression according to claim 7, characterized in that: The geranium essential oil is extracted from geranium leaves by steam distillation; Among them, measured by gas chromatography, in terms of mass percentage, the content of citronellol in the geranium essential oil is 35%-50%, and the content of geraniol is 15%-25%.

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