Traditional Chinese medicine composition containing bezoar for oral cavity as well as preparation method and application of traditional Chinese medicine composition

Abstract

The invention discloses a calculus bovis-containing traditional Chinese medicine composition for an oral cavity and a preparation method and application of the calculus bovis-containing traditional Chinese medicine composition. The traditional Chinese medicine composition is prepared from the following raw material medicines in parts by weight: 0.05 to 3 parts of calculus bovis, 0.3 to 5 parts of borneol, 3 to 25 parts of Chinese soapberry fruit, 3 to 25 parts of herba taxilli, 4 to 10 parts of honeysuckle flower and 0.4 to 4 parts of herba houttuyniae. According to the traditional Chinese medicine composition provided by the invention, by elaborately compounding various components such as the Chinese tamarisk tree, the Chinese soapberry fruit, the honeysuckle flower, the heartleaf houttuynia herb and the borneol, mutual promotion and synergistic interaction among the components are realized. The composition can effectively inhibit the formation of dental plaque, reduce the gingival index and relieve the symptoms of gingivitis, and shows high safety and convenient use experience at the same time. Due to the multifunctionality, the diversified requirements of consumers are met, and the remarkable public health and commercial value is manifested.

Description

Technical Field

[0001] The invention relates to the technical field of toothpaste, and in particular to a bezoar-containing traditional Chinese medicine composition for oral cavity and a preparation method and application thereof. Background Art

[0002] Dental plaque refers to the microbial community that adheres tightly to the tooth surface and adjacent surfaces, mainly composed of bacteria, oral epithelial cell shedding, food residues, and a small amount of saliva. The accumulation of this microbial film is not only a precursor to the formation of dental calculus, but also a key factor in inducing periodontal diseases such as gingivitis, periodontitis and caries. It can cause a series of uncomfortable symptoms such as bleeding gums, bad breath (halitosis), swollen and painful gums, and seriously affect oral health and quality of life.

[0003] Toothpaste, as an indispensable oral cleaning and care product in daily life, plays a vital role in maintaining oral health. With the improvement of social and economic levels and the improvement of quality of life, consumers' demand for oral care products is becoming increasingly diversified, and they are particularly inclined to choose products that are in line with the concept of natural health and have definite effects. Chinese medicine toothpaste, with its natural ingredients and potential health benefits, just fits the psychological expectations of consumers who pursue closeness to nature, safety and effectiveness. Therefore, toothpaste containing a variety of Chinese medicine extracts has gradually gained wide recognition and trust in the market.

[0004] However, although there are many Chinese medicine toothpastes available on the market, their formulas are often relatively simple, which makes it difficult to fully meet consumers' diversified needs for oral health, and their effects in inhibiting plaque growth, reducing gingival inflammation index, and relieving gingivitis symptoms still need to be improved. In view of this, the development of a new Chinese medicine composition for maintaining oral health, through the scientific combination of a variety of Chinese medicine ingredients with clear antibacterial and anti-inflammatory effects, in order to achieve a more significant effect of inhibiting plaque, effectively reducing the degree of gingival inflammation, and alleviating gingivitis-related symptoms, has become an important issue that needs to be urgently addressed in the current oral care field. Summary of the invention

[0005] The present invention aims to solve at least one of the technical problems existing in the prior art. To this end, the present invention provides a Chinese medicine composition for oral cavity, which can significantly inhibit dental plaque effect and effectively reduce the degree of gingival inflammation.

[0006] The invention also provides a method for preparing the Chinese medicine composition.

[0007] The invention also provides the application of the traditional Chinese medicine composition.

[0008] The invention also provides a toothpaste containing the traditional Chinese medicine composition.

[0009] The present invention also provides a method for preparing a toothpaste paste by using the traditional Chinese medicine composition.

[0010] According to the first aspect of the present invention, the Chinese medicine composition for oral cavity, measured by weight, the raw materials of the Chinese medicine composition include: 0.05-3 parts of bezoar, 0.3-5 parts of borneol, 3-25 parts of soapberry, 3-25 parts of toothbrush tree, 4-10 parts of honeysuckle and 0.4-4 parts of houttuynia cordata.

[0011] The Chinese medicine composition according to the embodiment of the present invention has at least the following beneficial effects: the Chinese medicine composition proposed in the present invention achieves mutual promotion and synergistic enhancement among the components by carefully compounding multiple components such as toothbrush tree, soapberry, honeysuckle, houttuynia cordata, borneol, etc. The composition can effectively inhibit the formation of dental plaque, reduce the gingival index, relieve the symptoms of gingivitis, and at the same time show a high degree of safety and convenient use experience. Its multifunctionality not only meets the diverse needs of consumers, but also demonstrates significant public health and commercial value. In traditional Chinese medicine theory, gingivitis is classified into the categories of "toothache", "tooth bleeding", "tooth bleeding", etc., and its pathogenesis is mostly attributed to the accumulation of heat in the stomach, the bladder fire, and the steaming of damp heat. Based on this traditional theory, the honeysuckle in the present invention, as the monarch medicine, plays a core therapeutic role by virtue of its characteristics of dispersing wind-heat, clearing heat and detoxifying, and eliminating filth and turbidity. Houttuynia cordata, as a ministerial medicine, strengthens the ability of honeysuckle to evacuate the upper heat evil by clearing heat and detoxifying, reducing swelling and relieving pain, especially for the removal of bladder damp heat. As an adjuvant, bezoar, with its expertise in clearing the heart and detoxifying, is specifically targeted at the heat of the heart and stomach, and has a significant detoxification, swelling and analgesic effect on symptoms such as swollen and painful gums, loose teeth and bleeding teeth caused by blazing stomach fire. Borneol, also as an adjuvant, can clear the mind, clear turbidity and relieve depression, clear heat and relieve pain, and effectively reduce the bitterness of traditional Chinese medicine, and is a commonly used medicinal material for otolaryngology. Sapindus mukorossi and toothbrush tree, with their powerful decontamination ability, play an important role in cleaning the oral cavity, preventing caries, whitening teeth and assisting in antibacterial and anti-inflammatory effects in the composition, further improving the efficacy of the Chinese medicine composition.

[0012] According to some embodiments of the present invention, bezoar is a highly bionic bezoar. Bezoar is a dried gallstone of the bovine animal Bos taurusdomesticus Gmelin, which is sweet and cool in nature and enters the heart and liver meridians. Bezoar was first recorded in Shennong's Herbal Classic and is ranked as a top grade. It has the functions of clearing the heart, removing phlegm, opening the mind, cooling the liver, stopping wind, and detoxifying. It is used for fever, coma, stroke, phlegm, convulsions, epilepsy, sore throat, oral sores, carbuncle, furuncle. The probability of bezoar generation under natural conditions is only 0.1%-0.2%, so there is a saying among the people that "natural bezoar is more expensive than gold". In order to solve the problem of bezoar scarcity, as early as the 1970s, experts in animal husbandry, pharmacy, etc. across the country tackled the problem and proposed three solutions: "in vitro cultivation of bezoar", "cultivation of bezoar", and "artificial bezoar". However, due to objective reasons and technical bottlenecks, the problem of shortage of bezoar has not been completely solved so far. The patent application with the number 202410855888.0 applies synthetic biology technology to develop "highly bionic bezoar", which highly simulates the process of bezoar formation in the cow's body. Its composition is highly similar to natural bezoar and has obvious cost advantages. It can meet the medication needs of ordinary people to a certain extent, and also makes the application of "highly bionic bezoar" in the field of oral care possible.

[0013] According to some embodiments of the present invention, the preparation method of high bionic bezoar comprises the following steps:

[0014] Taking Escherichia coli, inoculating it in a mixed liquid culture medium containing (preferably in an equal volume ratio) LB broth culture medium, EC broth culture medium and tryptic soy bean culture medium, and culturing it at a constant temperature of 36-38° C. for 6-7 hours to obtain a fermentation broth containing Escherichia coli; taking fresh ox bile, inoculating the Escherichia coli fermentation broth (according to a volume ratio of 5-15%), and culturing it at a constant temperature of 36-38° C. for 48-96 hours to obtain fermented bile; mixing the fermented bile with a saturated calcium hydroxide supernatant (preferably in a volume ratio of 1:1-1:3), separating the solid and the liquid, and collecting the solid phase to obtain composite bilirubin calcium;

[0015] In a sodium hydroxide solution, bilirubin, sodium bisulfite and a saturated solution of calcium hydroxide are mixed, the solid and liquid are separated, and the solid phase is collected to obtain a bilirubin calcium precipitate;

[0016] Weigh bile acid, deoxycholic acid, taurine, magnesium sulfate, zinc sulfate and ferrous sulfate, add water, mix by ultrasonication, add complex calcium bilirubin and calcium bilirubin precipitate, mix evenly, adjust the pH to 6.0-6.5, and obtain the product.

[0017] According to some embodiments of the present invention, the raw materials of the traditional Chinese medicine composition are composed of 0.05-3 parts of bezoar, 0.3-5 parts of borneol, 3-25 parts of soapberry, 3-25 parts of toothbrush tree, 4-10 parts of honeysuckle and 0.4-4 parts of houttuynia cordata, by weight.

[0018] According to some embodiments of the present invention, the raw materials of the traditional Chinese medicine composition include, by weight: 0.05-1 parts of bezoar, 0.3-4 parts of borneol, 3-20 parts of soapberry, 3-20 parts of toothbrush tree, 4-8 parts of honeysuckle and 0.4-2 parts of houttuynia cordata.

[0019] According to some embodiments of the present invention, the traditional Chinese medicine composition includes the following components: honeysuckle extract, houttuynia cordata extract, soapberry extract, toothbrush tree extract, bezoar powder and borneol powder.

[0020] According to some embodiments of the present invention, the honeysuckle extract, houttuynia cordata extract, soapberry extract and dentalia odorata extract are all water extracts.

[0021] The preparation method according to the second aspect of the present invention comprises the following steps:

[0022] Preparation of soapberry extract and / or toothbrush tree extract: take soapberry peel and / or toothbrush tree trunk (toothbrush tree trunk includes at least one of root, branch or stem), add water and reflux to extract 2 to 3 times, each time for 1.5 to 3 hours, the solvent ratio is 1:5 to 1:10, combine the extracts of each extraction, separate the solid and liquid, collect the liquid phase and concentrate it to a relative density of 1.05 to 1.10, and obtain;

[0023] Preparation of honeysuckle extract and / or houttuynia cordata extract: take honeysuckle and / or houttuynia cordata, add water and reflux to extract 1 to 2 times, each time for 0.5 to 1 hour, the solvent ratio is 1:15 to 1:20, the extracts from each extraction are combined, the solid-liquid separation is performed, and the liquid phase is collected and concentrated to a relative density of 1.05 to 1.10 to obtain;

[0024] Preparation of bezoar powder and / or borneol powder: crush bezoar and / or borneol in an environment below 28°C (preferably 0-28°C, such as 5°C, 10°C, 15°C, 20°C, 25°C, etc.) and process at room temperature or low temperature to reduce the loss of effective ingredients in bezoar.

[0025] The soapberry extract and the toothbrush tree extract can be extracted and prepared together, or can be extracted separately and then mixed.

[0026] The honeysuckle extract and the houttuynia cordata extract can be extracted and prepared together, or can be extracted separately and then mixed.

[0027] Bezoar powder and borneol powder can be pulverized together for preparation, or they can be pulverized separately and then mixed.

[0028] According to some embodiments of the present invention, the soapberry peel is crushed to a particle size of less than 5 mm, the toothbrush tree trunk is cut into 2-5 mm, and then they are crushed together to pass through a 60-80 mesh sieve.

[0029] According to some embodiments of the present invention, the Houttuynia cordata is cut into a length of 1 to 5 mm before being extracted by water reflux.

[0030] According to some embodiments of the present invention, the soapberry peel is crushed to a particle size of ≤5 mm before adding water for reflux extraction, and then pulverized to pass through a 60-80 mesh sieve.

[0031] According to some embodiments of the present invention, the toothbrush tree trunks are cut into a length of 2 to 5 mm and crushed to pass through a 60 to 80 mesh sieve before adding water for reflux extraction.

[0032] According to some embodiments of the present invention, the particle size of the borneol powder is 150 mesh to 200 mesh.

[0033] According to some embodiments of the present invention, the particle size of the bezoar powder is 150 mesh to 200 mesh.

[0034] According to the application of the third aspect of the present invention, the above-mentioned Chinese medicine composition is used in the preparation of oral care products.

[0035] According to the application of the embodiment of the present invention, at least the following beneficial effects are achieved: the Chinese medicine composition of the present invention is mainly composed of Chinese medicines such as toothbrush tree, soapberry, honeysuckle, houttuynia cordata, and borneol, and these raw materials have high biological activity and safety. The formula of the Chinese medicine composition is based on the theory of traditional Chinese medicine, and is precisely treated for the pathogenesis of oral problems such as gingivitis. For example, honeysuckle and houttuynia cordata can clear away heat and detoxify, bezoar can clear the heart and detoxify, and borneol can clear away heat and relieve pain. These ingredients act together in the oral cavity to effectively relieve symptoms such as swollen and painful gums. By compounding a variety of Chinese herbal medicine ingredients, the composition can exert the synergistic effect between the ingredients and improve the therapeutic effect. This multi-target, multi-pathway treatment method makes oral care more comprehensive and in-depth.

[0036] According to some embodiments of the present invention, the oral care product includes at least one of toothpaste, tooth powder, and mouthwash. It can also be other novel oral care products. The oral care products made of the Chinese medicine composition, such as mouthwash, tooth powder, toothpaste, etc., are easy to use, have a good taste, and are easily accepted by consumers; at the same time, the product is small and portable, easy to carry, and improves the practicality and convenience of the product.

[0037] According to the toothpaste of the fourth aspect of the present invention, the toothpaste paste contains the above-mentioned traditional Chinese medicine composition and a toothpaste base.

[0038] According to some embodiments of the present invention, the mass proportion of the traditional Chinese medicine composition in the paste is 1 to 20%.

[0039] According to some embodiments of the present invention, the mass proportion of the traditional Chinese medicine composition in the paste is 5-15%.

[0040] According to some embodiments of the present invention, the toothpaste base includes a humectant, a thickener, an abrasive, a foaming agent and a stabilizer.

[0041] According to some embodiments of the present invention, the toothpaste base further comprises flavors, preservatives and sweeteners.

[0042] According to some embodiments of the present invention, the toothpaste paste is made of the following raw material components in parts by weight: 1% to 20% of traditional Chinese medicine additives, 25% to 65% of moisturizers, 0.4% to 1% of thickeners, 10% to 60% of abrasives, 1% to 2% of foaming agents, 0.5% to 2% of flavors, 0.1% to 0.5% of preservatives, 0.05% to 0.4% of sweeteners, 0.2% to 5% of stabilizers, and the balance is water.

[0043] According to some embodiments of the present invention, the toothpaste paste is made of the following raw material components in parts by weight: 5% to 15% of traditional Chinese medicine additives, 25% to 65% of moisturizers, 0.4% to 1% of thickeners, 10% to 40% of abrasives, 1% to 2% of foaming agents, 0.5% to 2% of flavors, 0.1% to 0.5% of preservatives, 0.05% to 0.4% of sweeteners, 0.2% to 5% of stabilizers, and the balance is water.

[0044] According to some embodiments of the present invention, the friction agent is at least one of calcium carbonate, calcium hydrogen phosphate dihydrate, calcium pyrophosphate, hydrated silica, aluminum hydroxide, and calcium hydrogen phosphate.

[0045] According to some embodiments of the present invention, the humectant is at least one of sorbitol, glycerol, propylene glycol, and polyethylene glycol.

[0046] According to some embodiments of the present invention, the thickener is at least one of sodium carboxymethyl cellulose, xanthan gum, hydroxyethyl cellulose, guar gum, and carrageenan.

[0047] According to some embodiments of the present invention, the foaming agent is at least one of sodium lauryl sulfate, sodium lauryl sulfate, and sodium lauroyl sarcosinate.

[0048] According to some embodiments of the present invention, the sweetener is at least one of saccharin sodium, acesulfame potassium, and sucralose.

[0049] According to some embodiments of the present invention, the preservative is at least one of methylparaben or its sodium salt, ethylparaben or its sodium salt, and sodium benzoate.

[0050] According to some embodiments of the present invention, the stabilizer is at least one of tetrasodium pyrophosphate, sodium dihydrogen phosphate, and trisodium phosphate.

[0051] According to some embodiments of the present invention, the flavor is at least one of mint flavor, lemon flavor, sweet orange flavor, and tangerine flavor.

[0052] According to the fifth aspect of the present invention, the preparation method of the toothpaste paste comprises the following steps: mixing bezoar, borneol and abrasive to obtain a bezoar-borneol-abrasive mixture; mixing other ingredients in the traditional Chinese medicine composition with a moisturizer, a sweetener and a preservative, adding water, and then adding the bezoar-borneol-abrasive mixture, a thickener, a foaming agent and a stabilizer, mixing, grinding, degassing, adding flavor, mixing, and grinding again to obtain the toothpaste paste.

[0053] According to some embodiments of the present invention, the preparation method includes the following steps: mixing bezoar powder, borneol powder and abrasive to obtain a bezoar-borneol-abrasive mixture; mixing soapberry extract, toothbrush tree extract, honeysuckle extract, Houttuynia cordata extract with a moisturizer, a sweetener and a preservative, adding water, and then adding the bezoar-borneol-abrasive mixture, a thickener, a foaming agent and a stabilizer, mixing, grinding, degassing, adding flavor, mixing, and grinding again to obtain.

[0054] According to some embodiments of the present invention, the bezoar and borneol are mixed and prepared into powder before adding; the soapberry and toothbrush tree in the traditional Chinese medicine composition are mixed and extracted before adding, and the honeysuckle and houttuynia cordata are mixed and extracted before adding.

[0055] The paste is aseptically filled into a soft tube, sealed and packaged to prepare the toothpaste.

[0056] Additional aspects and advantages of the present invention will be given in part in the following description and in part will be obvious from the following description, or will be learned through practice of the present invention. DETAILED DESCRIPTION

[0057] The following will be combined with the embodiments to clearly and completely describe the concept of the present invention and the technical effects produced, so as to fully understand the purpose, characteristics and effects of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of them. Based on the embodiments of the present invention, other embodiments obtained by technicians in this field without creative work are all within the scope of protection of the present invention. The test methods used in the embodiments are conventional methods unless otherwise specified; the materials, reagents, etc. used, unless otherwise specified, can be reagents and materials obtained from commercial channels. Unless otherwise specified, the same parameter in each embodiment has the same value. The embodiments described below are exemplary and are only used to explain the present invention, and cannot be understood as limitations on the present invention.

[0058] In the description of the present invention, the description with reference to the term "some embodiments" or the like means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner.

[0059] In the description of the present invention, if there is a description of first, second, etc., it is only for the purpose of distinguishing technical features, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features or implicitly indicating the order of the indicated technical features.

[0060] In the following examples, natural Chinese medicinal materials contain inevitable impurities, and the impurity content does not exceed 1%.

[0061] Example 1

[0062] This example provides a traditional Chinese medicine composition, the raw materials of which are composed of the following components by weight: 0.05 parts of high-biosimilar bezoar, 0.3 parts of borneol, 3 parts of soapberry, 3 parts of toothbrush tree, 4 parts of honeysuckle, and 0.4 parts of houttuynia cordata.

[0063] The preparation process of the Chinese medicine composition is as follows:

[0064] Step 1:

[0065] The preparation method of high bionic bezoar is as follows: taking Escherichia coli (purchased from China Center for Type Culture Collection, with a collection number of CCTCC AB 2012885), inoculating it into a mixed liquid culture medium containing LB broth culture medium, EC broth culture medium and tryptic soy bean culture medium in equal volume ratio, and culturing it at a constant temperature of 36-38°C for 6-7h to obtain a fermentation broth containing Escherichia coli (OD600 value is measured by ultraviolet spectrophotometer, and OD600 value is 2-3); taking fresh bovine bile, inoculating Escherichia coli fermentation broth at a volume ratio of 8%, and culturing it at a constant temperature of 36-38°C for 48h to obtain fermented bile; mixing the fermented bile with a saturated calcium hydroxide supernatant at a volume ratio of 1:2, stirring, standing, centrifuging, collecting the precipitate, and drying to obtain composite bilirubin calcium; The weight of the obtained composite bilirubin calcium is recorded as a. In a 10nM sodium hydroxide solution, 50-100% a weight of bilirubin, 0.05%-1% a sodium bisulfite and a saturated calcium hydroxide solution are mixed, centrifuged, and the precipitate is collected to obtain the bilirubin calcium precipitate; 20%-30% a bile acid, 10-20% a deoxycholic acid, 10-20% a taurine, 1-5% a magnesium sulfate, 1-5% a zinc sulfate, and 0.1-1% a ferrous sulfate are weighed, and purified water is ultrasonically dissolved, and the composite bilirubin calcium and bilirubin calcium precipitate obtained by the above operation are added, mixed evenly, the pH is adjusted to 6.0-6.5, and dried to obtain a high bionic bezoar. According to the weight percentage, C 33 H 36 The bilirubin content in the high bionic bezoar was measured to be 25.2% based on N4O6; 24 H 40 O5 meter, the bile acid content in the high bionic bezoar was measured to be 4.1%.

[0066] Step 2:

[0067] Take soapberry peel, remove impurities, crush to particle size ≤5mm with crusher, grind with pulverizer, and pass through 60 mesh sieve; take toothbrush tree branches, remove impurities, cut to length 2-5mm with reciprocating medicine cutter, grind with pulverizer, and pass through 60 mesh sieve. Mix the two, add water and reflux extraction twice, each time for 1.5 hours, the solvent ratio is 1:5, the extracts are combined, filtered through 180 mesh sieve, the filtrate is concentrated under reduced pressure to a relative density of 1.05, spray dried, and grinded into 120 mesh fine powder to obtain soapberry-toothbrush tree extract;

[0068] Take honeysuckle and houttuynia cordata, remove impurities, cut houttuynia cordata into 1-5 mm in length, mix the two, add water and reflux to extract once, each time for 0.5 hour, the solvent ratio is 1:15, combine the extracts, filter through a 180-mesh sieve, decompress and concentrate the filtrate to a relative density of 1.10, spray dry, and crush into 120-mesh fine powder to obtain honeysuckle-houttuynia cordata extract;

[0069] Step 3:

[0070] Take high bionic bezoar and borneol and mix them, grind them into ultrafine powder at 0℃ by ball milling to make 200 mesh fine powder, that is, bezoar-borneol ultrafine powder.

[0071] When the above Chinese medicine composition is prepared into toothpaste, its components are shown in Table 1 below:

[0072] Table 1 Components of toothpaste

[0073]

[0074]

[0075] Preparation process:

[0076] The bezoar-borneol ultrafine powder is mixed with the abrasive to obtain a bezoar-borneol-abrasive mixture; the soapberry-toothbrush tree extract, the honeysuckle-houttuynia cordata extract, the moisturizer, the sweetener, the preservative are mixed and put into deionized water, and stirred to prepare a deionized water solution, and then the bezoar-borneol-abrasive mixture and the thickener, the foaming agent, and the stabilizer are added, and the mixture is sent to a three-in-one vacuum paste making machine, and the mixture is stirred and ground at a high speed for 25 to 50 minutes, and vacuum degassing is performed, and then the essence is added, stirred and ground for 10 to 20 minutes, and mixed to obtain a toothpaste paste, and the toothpaste paste is aseptically filled into a soft tube, sealed, and packaged.

[0077] Example 2

[0078] This example provides a traditional Chinese medicine composition, the raw materials of which are composed of the following components by weight: 0.1 parts of high-biosimilar bezoar, 4 parts of borneol, 20 parts of soapberry, 20 parts of toothbrush tree, 8 parts of honeysuckle, and 2 parts of houttuynia cordata.

[0079] The Chinese medicine additive comprises the following steps:

[0080] Step 1:

[0081] The preparation method of highly bionic bezoar is as follows: taking Escherichia coli (purchased from China Center for Type Culture Collection, with a collection number of CCTCC AB 2012885), inoculating it in a mixed liquid culture medium containing LB broth culture medium, EC broth culture medium and tryptic soy bean culture medium in equal volume ratio, and culturing it at a constant temperature of 36-38°C for 6-7h to obtain a fermentation broth containing Escherichia coli (OD600 value is measured by ultraviolet spectrophotometer, and OD600 value is 2-3); taking fresh bovine bile, inoculating Escherichia coli fermentation broth at a volume ratio of 15%, and culturing it at a constant temperature of 36-38°C for 96h to obtain fermented bile; mixing the fermented bile with a saturated calcium hydroxide supernatant at a volume ratio of 1:3, stirring, standing, centrifuging, collecting the precipitate, and drying to obtain complex bilirubin. calcium; the weight of the obtained composite bilirubin calcium is recorded as a, in 10nM sodium hydroxide solution, 50-100% a weight of bilirubin, 0.05%-1% a sodium bisulfite and calcium hydroxide saturated solution are mixed, centrifuged, and the precipitate is collected to obtain the bilirubin calcium precipitate; weigh 20%-30% a bile acid, 10-20% a deoxycholic acid, 10-20% a taurine, 1-5% a magnesium sulfate, 1-5% a zinc sulfate, 0.1-1% a ferrous sulfate, and dissolve them in purified water by ultrasonication, add the composite bilirubin calcium and bilirubin calcium precipitate obtained by the above operation, mix evenly, adjust the pH to 6.0-6.5, and dry to obtain. According to weight percentage, C 33 H 36 The bilirubin content in the bezoar was measured to be 25.4% based on N4O6. 24 H 40 O5 meter, the bile acid content in the bezoar was measured to be 4.3%.

[0082] Step 2:

[0083] Take soapberry peel, remove impurities, crush with a crusher to a particle size of ≤5mm, grind with a pulverizer, and pass through an 80-mesh sieve; take toothbrush tree branches, remove impurities, cut with a reciprocating medicine cutter to a length of 2-5mm, grind with a pulverizer, and pass through an 80-mesh sieve. Mix the two, add water and reflux to extract 3 times, each time for 3 hours, with a solvent ratio of 1:10, combine the extracts, filter through a 200-mesh sieve, decompress and concentrate the filtrate to a relative density of 1.10, spray dry, and grind into 120-mesh fine powder to obtain soapberry-toothbrush tree extract;

[0084] Take honeysuckle and houttuynia cordata, remove impurities, cut houttuynia cordata into 1-5 mm in length, mix the two, add water and reflux to extract twice, each time for 1 hour, the solvent ratio is 1:20, combine the extracts, filter through a 200-mesh sieve, decompress and concentrate the filtrate to a relative density of 1.05, spray dry, and crush into 120-mesh fine powder to obtain honeysuckle-houttuynia cordata extract;

[0085] Step 3:

[0086] Mix high-biomimetic bezoar and borneol, and grind them into ultrafine powder at 28°C by ball milling to obtain 150-mesh fine powder, thus obtaining bezoar-borneol ultrafine powder;

[0087] When the above Chinese medicine composition is prepared into toothpaste, its components are shown in Table 2 below:

[0088] Table 2 Components of toothpaste

[0089]

[0090]

[0091] Preparation process:

[0092] The bezoar-borneol ultrafine powder is mixed with the abrasive to obtain a bezoar-borneol-abrasive mixture; the soapberry-toothbrush tree extract, the honeysuckle-houttuynia cordata extract, the moisturizer, the sweetener, the preservative are mixed and put into deionized water, and stirred to prepare a deionized water solution, and then the bezoar-borneol-abrasive mixture and the thickener, the foaming agent, and the stabilizer are added, and the mixture is sent to a three-in-one vacuum paste making machine, and the mixture is stirred and ground at a high speed for 25 to 50 minutes, and vacuum degassing is performed, and then the essence is added, stirred and ground for 10 to 20 minutes, and mixed to obtain a toothpaste paste, and the toothpaste paste is aseptically filled into a soft tube, sealed, and packaged.

[0093] Example 3

[0094] This example provides a traditional Chinese medicine composition, the raw materials of which are composed of the following components by weight: 0.08 parts of high-biosimilar bezoar, 2 parts of borneol, 12 parts of soapberry, 12 parts of toothbrush tree, 6 parts of honeysuckle, and 1.2 parts of houttuynia cordata.

[0095] The preparation process of the Chinese medicine composition is as follows:

[0096] Step 1:

[0097] The preparation method of highly bionic bezoar is as follows: taking Escherichia coli (purchased from China Center for Type Culture Collection, with a collection number of CCTCC AB 2012885), inoculating it in a mixed liquid culture medium containing LB broth culture medium, EC broth culture medium and tryptic soy bean culture medium in equal volume ratios, and culturing it at a constant temperature of 36 to 38°C for 6 to 7 hours to obtain a fermentation broth containing Escherichia coli (the OD600 value is measured by an ultraviolet spectrophotometer, and the OD600 value is about 2 to 3); taking fresh bovine bile, inoculating the Escherichia coli fermentation broth at a volume ratio of 5%, and fermenting and culturing it at a constant temperature of 36 to 38°C for 72 hours to obtain fermented bile; mixing the fermented bile with a saturated calcium hydroxide supernatant at a volume ratio of 1:1, stirring, standing, centrifuging, collecting the precipitate, and drying to obtain compound bilirubin. Calcium bilirubin; the weight of the obtained composite bilirubin calcium is recorded as a, in 10nM sodium hydroxide solution, 50-100% a weight of bilirubin, 0.05%-1% a sodium bisulfite and calcium hydroxide saturated solution are mixed, centrifuged, and the precipitate is collected to obtain bilirubin calcium precipitate; 20%-30% a bile acid, 10-20% a deoxycholic acid, 10-20% a taurine, 1-5% a magnesium sulfate, 1-5% a zinc sulfate, 0.1-1% a ferrous sulfate are weighed, and purified water is ultrasonically dissolved, and the composite bilirubin calcium and bilirubin calcium precipitate obtained by the above operation are added, mixed evenly, the pH is adjusted to 6.0-6.5, and dried to obtain. According to weight percentage, C 33 H 36 The bilirubin content in the bezoar was measured to be 28.1% based on N4O6; 24 H 40 O5 meter, the bile acid content in the bezoar was measured to be 5.2%.

[0098] Step 2:

[0099] Take soapberry peel, remove impurities, crush with a crusher to a particle size of ≤5mm, grind with a pulverizer, and pass through an 80-mesh sieve; take toothbrush tree branches, remove impurities, cut with a reciprocating medicine cutter to a length of 2-5mm, grind with a pulverizer, and pass through an 80-mesh sieve. Mix the two, add water and reflux to extract twice, each time for 2 hours, with a solvent ratio of 1:10, combine the extracts, filter through a 200-mesh sieve, decompress and concentrate the filtrate to a relative density of 1.10, spray dry, and grind into 120-mesh fine powder to obtain soapberry-toothbrush tree extract;

[0100] Take honeysuckle and houttuynia cordata, remove impurities, cut houttuynia cordata into 1-5 mm in length, mix the two, add water and reflux to extract once, each time for 0.5 hour, the solvent ratio is 1:15, filter through a 200-mesh sieve, decompress and concentrate the filtrate to a relative density of 1.10, spray dry, and crush into 120-mesh fine powder to obtain a honeysuckle-houttuynia cordata extract;

[0101] Step 3:

[0102] Mix high-biomimetic bezoar and borneol, and grind them into 200-mesh fine powder by ball milling at 10°C to obtain bezoar-borneol ultrafine powder;

[0103] When the above Chinese medicine composition is prepared into toothpaste, its components are shown in Table 3 below:

[0104] Table 3 Components of toothpaste

[0105]

[0106] Example 4

[0107] In this example, a toothpaste was prepared. The preparation process of the toothpaste was different from that of the toothpaste in Example 3 only in that in step (iii), the temperature of the ball milling ultrafine grinding was 38°C, and the other operations were the same as those in Example 3.

[0108] Example 5

[0109] In this example, a toothpaste is prepared. The preparation process is different from that of the toothpaste in Example 3 only in that the extraction method of soapberry, toothbrush tree, honeysuckle and houttuynia cordata in the raw material components is changed from fractional extraction to mixed extraction. Take soapberry peel, remove impurities, crush it with a crusher to a particle size of ≤5mm, grind it with a pulverizer, and pass through an 80-mesh sieve; take toothbrush tree branches, remove impurities, cut them to a length of 2-5mm with a reciprocating medicine cutter, grind them with a pulverizer, and pass through an 80-mesh sieve. Take honeysuckle and houttuynia cordata, remove impurities, and cut houttuynia cordata to a length of 1-5mm. Mix the four, add water and reflux to extract twice, each time for 1.5 hours, with a solvent ratio of 1:10, combine the extracts, filter through a 200-mesh sieve, and concentrate the filtrate under reduced pressure to a relative density of 1.10, spray dry, and grind it into a 120-mesh fine powder to obtain a soapberry-toothbrush tree-honeysuckle-houttuynia cordata extract. Other operations are the same as in Example 3.

[0110] Example 6

[0111] In this example, a toothpaste was prepared. The preparation process of the toothpaste was different from that of the toothpaste in Example 3 only in that the extraction parameters of soapberry, toothbrush tree, honeysuckle and houttuynia cordata in the raw material components were changed.

[0112] Take soapberry peel, remove impurities, crush with a crusher to a particle size of ≤5mm, grind with a pulverizer, and pass through an 80-mesh sieve; take toothbrush tree branches, remove impurities, cut with a reciprocating medicine cutter to a length of 2-5mm, grind with a pulverizer, and pass through an 80-mesh sieve. Mix the two, add water and reflux to extract twice, each time for 1 hour, with the solvent ratio of 1:10, combine the extracts, filter through a 200-mesh sieve, decompress and concentrate the filtrate to a relative density of 1.10, spray dry, and grind into 120-mesh fine powder to obtain soapberry-toothbrush tree extract;

[0113] Take honeysuckle and houttuynia cordata, remove impurities, cut houttuynia cordata into a length of 1-5 mm, mix the two, add water and reflux to extract twice, each time for 1.5 hours, the solvent ratio is 1:10, filter through a 200-mesh sieve, and decompress the filtrate to a relative density of 1.10, spray dry, and crush into 120-mesh fine powder to obtain the honeysuckle-houttuynia cordata extract.

[0114] Comparative Example 1

[0115] In this example, a toothpaste is prepared, and the preparation process thereof is different from that of the toothpaste in Example 3 only in that the raw material components do not contain high bionic bezoar.

[0116] Comparative Example 2

[0117] In this example, a toothpaste was prepared, and the preparation process thereof was different from that of the toothpaste in Example 3 only in that the raw material components did not contain borneol.

[0118] Comparative Example 3

[0119] In this example, a toothpaste was prepared, and the preparation process thereof was different from that of the toothpaste in Example 3 only in that the raw material components did not contain honeysuckle.

[0120] Comparative Example 4

[0121] In this example, a toothpaste is prepared. The preparation process of the toothpaste is different from that of the toothpaste in Example 3 only in that the raw material components do not contain Houttuynia cordata.

[0122] Comparative Example 5

[0123] In this example, a toothpaste is prepared. The preparation process of the toothpaste is different from that of the toothpaste in Example 3 only in that the raw material components do not contain soapberry and toothbrush tree.

[0124] Comparative Example 6

[0125] In this example, a toothpaste was prepared, and the preparation process thereof was different from that of the toothpaste in Example 3 only in that: in the raw material drug component, each formula was composed of the following components by weight: 0.01 part of high bionic bezoar, 0.1 part of borneol, 1 part of soapberry, 1 part of toothbrush tree, 1 part of honeysuckle, and 0.1 part of houttuynia. The other operations were the same as in Example 3.

[0126] Comparative Example 7

[0127] In this example, a toothpaste is prepared. The preparation process thereof is different from that of the toothpaste in Example 3 in that it does not contain the Chinese medicine composition but only contains the toothpaste base.

[0128] In order to verify the effect of the toothpaste prepared by the above operation, its performance was tested as follows:

[0129] Test Example 1: Security Test

[0130] 1.1 Acute oral toxicity test

[0131] Conduct acute oral toxicity tests in accordance with the Technical Specifications for Safety of Cosmetics (2015 Edition).

[0132] 50 healthy ICR mice, half male and half female, weighing 18-20g, were randomly divided into 5 groups, of which 1 group was the control group, and the remaining 4 groups were different dose groups, with 10 mice in each group. The Chinese medicine toothpaste of Example 3 of the present invention was added with distilled water to form a 30% suspension, and the mice were given the medicine according to the maximum allowable capacity. The doses converted into the original toothpaste amount were 18, 14, 10, and 6 (g / kg) respectively. After the animals were fasted (without water) for 18 hours, they were given twice within 1 day (half an hour apart), but combined as a single dose, and the control group was given the same dose of distilled water. Observe the general state of the animals, toxicity manifestations, and the number of dead animals 4h, 24h, 48h, and 72h after oral administration.

[0133] Results: During the 72h observation period, when the mice were given the maximum allowable concentration and the maximum allowable volume (18g / kg·d), no mouse death was observed. The appetite, activity, fur color, mental state, etc. of the mice were not significantly different from those of the control group, that is, there was no acute systemic toxicity reaction. According to the oral toxicity classification, LD50 (mg / kg)>5000 is a practical non-toxic substance. The maximum dose of this test is 18g / kg·d, which is much greater than 5000mg / kg·d. Therefore, the Chinese medicine toothpaste of Example 3 of the present invention is safe, which further illustrates that the toothpaste provided by the present invention is safe.

[0134] 1.2 Oral mucosal irritation test

[0135] The oral mucosal irritation test was carried out with reference to GB / T 16886.10-2017.

[0136] 20 golden hamsters, half male and half female, weighing 110-130g, healthy, with no lesions on the bilateral cheek pouch mucosa of the oral cavity, were randomly divided into 2 groups, one of which was a toothpaste base group and the other was a toothpaste group. The Chinese medicinal toothpaste (diameter not more than 5mm) of Example 3 of the present invention was placed in the left cheek pouch of the hamster, and the toothpaste was not placed in the right cheek pouch as a control. Repeated once every 1h for 4 consecutive times, and the control group was given an equal amount of the toothpaste base group. Observe and record the changes in the systemic condition and local mucosa 24h after administration, and observe the local reactions such as congestion, swelling, erosion, and ulcers of the oral mucosa for 7d with the naked eye. The buccal mucosa and its surrounding tissues were taken for pathological histological section examination the next day after the last administration, and the abnormalities of the epithelial cell structures of each mucosa were observed. According to the classification of the mucosal irritation reaction of the naked eye and histopathological irritation reaction, the irritation of the toothpaste to the oral mucosa was evaluated.

[0137] Results: After 24 hours of administration, no abnormal systemic condition was found. Microscopic observation showed that the tissue epithelial cells and fibroblasts of the toothpaste group were intact, the cell nuclei were obvious, and no inflammatory cell infiltration was observed. Naked eye and tissue section observation showed that there was no abnormality between the toothpaste group and the matrix control group, indicating that the traditional Chinese medicine toothpaste of Example 3 of the present invention is non-irritating to the oral mucosa, and further indicating that the toothpaste provided by the present invention is non-irritating to the oral mucosa.

[0138] Test Example 2: Efficacy Test

[0139] 2.1 Determination of antibacterial effect

[0140] 2.1.1 Test method: The antibacterial test of bezoar traditional Chinese medicine toothpaste on Streptococcus mutans was carried out to observe the direct antibacterial effect of bezoar traditional Chinese medicine toothpaste on Streptococcus mutans.

[0141] Take the toothpaste obtained in Examples 1 to 6 and Comparative Examples 1 to 7, add distilled water at a ratio of toothpaste to water = 1:4 (weight ratio), take the leaching liquid as the test liquid, conduct an antibacterial test, detect the antibacterial rate, and calculate the antibacterial rate according to the following formula:

[0142] X=(A-B) / A×100%……(A1)

[0143] Where: X—inhibition rate, %;

[0144] A—average colony count of negative control group;

[0145] B—Average colony count of the tested samples.

[0146] 2.1.2 Test steps:

[0147] (1) Preparation of slant culture medium: Weigh 5 g of peptone, 3 g of beef extract, and 15 g of agar, dissolve in 1000 mL of distilled water, divide into test tubes, sterilize by autoclave at 121°C for 15 min, and place into slant medium.

[0148] (2) Preparation of bacterial suspension: Inoculate mutans Streptococcus on the slant culture medium in step (1) and culture for 24 h. Wash the bacterial lawn with PBS and adjust the bacterial suspension concentration to 4×10 4 cfu / mL.

[0149] (3) Experimental group: Take 3 tubes of 5 mL of the toothpaste sample solution prepared in Example, take the above bacterial suspension, add 100 μL of bacterial suspension to each sample solution, mix evenly, start timing, and let it act for 10 min. Use sterile tweezers to put the sample solution (0.5 mL) with bacterial suspension drops into test tubes containing 5 mL PBS, mix well, take 0.5 mL, and place it on three plates respectively. Pour 15 mL of MS culture medium, rotate the plate to make it fully uniform, turn the plate over after the agar solidifies, and culture at 37°C anaerobically under the following conditions: 80 vol% N2, 10 vol% CO2, 10 vol% H2. Count the number of live bacteria for 48 h and take the average value.

[0150] (4) Negative control group: Take 0.5 mL of bacterial suspension and put it into a test tube containing 5 mL of PBS. Mix thoroughly. Take 0.5 mL and place it on three plates respectively. Pour 15 mL of MS medium and rotate the plates to make it fully uniform. After the agar solidifies, turn the plates over and culture at 37°C anaerobically under the following conditions: 80 vol% N2, 10 vol% CO2, 10 vol% H2. Count the number of live bacteria for 48 h and take the average value.

[0151] (5) Comparative Example Test Group: The toothpastes of Comparative Examples 1 to 7 were subjected to the same operation as the experimental group.

[0152] 2.1.3 Results Analysis

[0153] The results are shown in Table 4.

[0154] Table 4 Antibacterial rate test analysis results

[0155]

[0156]

[0157] According to the evaluation standard of the antibacterial rate of the Technical Specification for Disinfection (2002 Edition), the antibacterial rate is 50-90%, and the product has an antibacterial effect; the antibacterial rate is ≥90%, and the product has a strong antibacterial effect. As shown in Table 4, Examples 1-3 have a strong antibacterial effect, and Examples 4-6 and Comparative Examples 1-6 all have an antibacterial effect, but the overall antibacterial rate of Comparative Examples 1-6 is significantly lower than that of Examples 1-6, indicating that the 6 kinds of Chinese medicine additives in the examples have a synergistic effect in antibacterial effect.

[0158] 2.2 Clinical effect observation

[0159] 2.2.1 Experimental method: In accordance with WS / T326.3-2010 "Toothpaste efficacy evaluation Part 3: Inhibition of dental plaque and (or) relief of gingival inflammation".

[0160] 2.2.2 Experimental design principles: The principle of randomized, double-blind, parallel controlled design was adopted. The subjects were randomly given toothpaste samples of Example 3 (experimental group) and Comparative Example 7 (control group) according to the screening order, and there were 40 subjects for each toothpaste.

[0161] 2.2.3 Subject selection:

[0162] 2.2.3.1 Inclusion criteria

[0163] a) In good general health, without major systemic diseases, and with more than 20 detectable teeth.

[0164] b) Aged between 18 and 60.

[0165] c) If female, she must not be pregnant or breastfeeding.

[0166] d) Subjects should have an appropriate degree of plaque or gingivitis.

[0167] e) Not participating in other similar experimental studies at the same time.

[0168] f) Sign the informed consent form and be able to complete the clinical trial as required.

[0169] 2.2.3.2 Exclusion criteria

[0170] a) History of allergy to test toothpaste ingredients.

[0171] b) Currently taking medication that may affect the test results.

[0172] c) Use of antibiotics one month before study participation.

[0173] d) There are open caries or mucosal lesions in the oral cavity.

[0174] e) Suffering from severe periodontitis.

[0175] 2.2.4 Experimental process

[0176] Each subject brushed his teeth with the toothpaste of this test in the morning and evening, using 1 to 2 g each time and brushing for 3 to 5 minutes each time.

[0177] 2.2.5 Trial period

[0178] The trial period is 3 months. To strengthen the compliance of the subjects, products and toothbrushes are distributed once a month to supervise and timely understand adverse reactions. After 1.5 months and 3 months, the subjects need to return for relevant record evaluation and soft tissue examination.

[0179] 2.2.6 Inspection indicators

[0180] Plaque index (such as the modified Quigley-Hein PI by Turesky et al.): Check the mesial, middle, and distal surfaces of each buccal and lingual tooth.

[0181] Gingivitis index (modified Loe-Silness gingivitis index, GI): Examine the buccal and lingual mesial, mesial, and distal surfaces of each tooth.

[0182] PI and GI were examined by 2 physicians who were self-calibrated and qualified. After the baseline PI examination, all tooth surfaces were de-plaqued using a rubber ring and polishing paste.

[0183] 2.2.7 Experimental results

[0184] The experimental results are shown in Table 5.

[0185] Table 5 Comparison of plaque index (PI) and gingivitis index (GI) at different time points

[0186]

[0187] The results showed that at the baseline, there was no significant difference in the plaque index (PI) and gingivitis index (GI) between the experimental group and the control group. After 1.5 months, there was no significant difference in the decrease of the plaque index (PI) and gingivitis index (GI) between the experimental group and the control group.

[0188] After 3 months, there was a significant difference in the decrease of plaque index (PI) and gingivitis index (GI) between the experimental group and the control group (p<0.05), which showed clinical efficacy in inhibiting dental plaque and alleviating gingivitis. No adverse reactions occurred during the entire trial.

[0189] The clinical experimental results show that the toothpaste prepared in Example 3 has a clinical effect of significantly inhibiting dental plaque and reducing gingivitis, and its effect is significantly better than that of the negative control group. At the same time, it can be predicted that other examples have the same effect as Example 3, and can also significantly inhibit dental plaque and reduce gingivitis.

[0190] Bezoar is the dried gallstone of Bos taurus domesticus Gmelin, a bovine animal. It is sweet and cool in nature, and enters the heart and liver meridians. It can clear the heart, dissipate phlegm, open the mind, cool the liver, calm the wind, and detoxify. It is used for fever, coma, stroke, convulsions, epilepsy, sore throat, mouth sores, carbuncle, furuncle. High bionic bezoar is made from fresh bile of bovine animals, combined with microbial fermentation technology to obtain the effective ingredients in bile, and added with bilirubin, bile acid, etc. The ingredients are highly similar to natural bezoar, and the original heat-clearing, detoxifying, anti-inflammatory and analgesic effects of natural bezoar are retained.

[0191] The toothbrush tree is a plant of the genus Salvadora persica L. of the family Salviae Crucifolia. As early as 4,000 years ago, in tropical Africa and Arabia, local tribesmen cleaned their mouths by chewing its branches. The toothbrush branches have a long history as a natural oral care tool. Using toothbrush branches to clean the mouth has the effects of strengthening and protecting teeth, preventing caries and whitening. After use, the mouth is refreshed and comfortable, and it is also natural and environmentally friendly.

[0192] Soapberry is a plant of the genus Sapindus delavayi of the family Sapindaceae. Its peel can be used as a soap substitute to produce abundant foam and has strong detergency. Studies have shown that the main detergency component is sapindus saponin, which is an excellent natural surfactant, particularly suitable for washing high-grade silk and pure wool fabrics and baby products, and in daily necessities such as bathing toothpaste. In addition, sapindus saponin also has a strong antibacterial effect.

[0193] Honeysuckle is the dried buds or flowers of Lonicera japonica Thunb. of the Caprifoliaceae family. It is sweet and cold. It enters the lung, heart and stomach meridians. It clears away heat and detoxifies, and dispels wind-heat. It is used for carbuncle, furuncle, throat paralysis, erysipelas, heat-toxic bloody dysentery, wind-heat cold, and fever caused by febrile diseases.

[0194] Houttuynia cordata is the fresh whole herb or dried aerial part of Houttuynia cordata Thunb. of the Saururaceae family. It is pungent and slightly cold. It enters the lung meridian. It clears away heat and detoxifies, eliminates carbuncle and discharges pus, and promotes diuresis and relieves stranguria. It is used for lung carbuncle vomiting pus, phlegm-heat cough, heat dysentery, heat stranguria, carbuncle, swelling and ulcer.

[0195] Borneol is made from fresh branches and leaves of Cinnamomum camphora (L.) Presl, a plant of the Lauraceae family, through extraction and processing. There are also chemical synthetic products. It is a white crystalline powder or flaky crystals. It has a light fragrance and tastes spicy and cool. Spicy, bitter, and slightly cold. It enters the heart, spleen, and lung meridians. It can open the mind and refresh the spirit, clear away heat and relieve pain. It is used for coma and convulsions caused by febrile diseases, syncope caused by phlegm, sudden syncope caused by qi stagnation, coma caused by poisoning, chest pain, heartache, red eyes, mouth sores, sore throat, and pus in the ear canal.

[0196] The effective ingredients in the Chinese medicine composition can inhibit the growth of bacteria in the oral cavity and reduce the formation of dental plaque, thereby preventing oral diseases such as caries and periodontal disease. By improving the inflammatory condition of the gums, the composition can significantly reduce the gum index and make the gums healthier. For the symptoms of gingivitis that have already appeared, the composition can quickly alleviate and reduce the pain of the patient. The addition of ingredients such as soapberry and toothbrush tree makes the composition have a strong cleaning ability, can remove dirt and tooth stains in the oral cavity, and reduce the formation of dental plaque.

[0197] It should be noted that the above embodiments all belong to the same inventive concept, and the description of each embodiment has its own focus. For details not described in individual embodiments, reference may be made to the description in other embodiments.

[0198] The embodiments of the present invention are described in detail above, but the present invention is not limited to the above embodiments. Various changes can be made within the knowledge scope of ordinary technicians in the relevant technical field without departing from the purpose of the present invention.

Claims

1. A Chinese medicine composition for oral cavity, characterized in that: The raw materials of the traditional Chinese medicine composition include, by weight, 0.05-3 parts of bezoar, 0.3-5 parts of borneol, 3-25 parts of soapberry, 3-25 parts of toothbrush tree, 4-10 parts of honeysuckle and 0.4-4 parts of houttuynia cordata.

2. The Chinese medicine composition for oral cavity according to claim 1, characterized in that: Bezoar is a highly bionic bezoar.

3. The Chinese medicine composition for oral cavity according to claim 1 or 2, characterized in that: The raw materials of the traditional Chinese medicine composition are composed of 0.05-3 parts of bezoar, 0.3-5 parts of borneol, 3-25 parts of soapberry, 3-25 parts of toothbrush tree, 4-10 parts of honeysuckle and 0.4-4 parts of houttuynia cordata by weight.

4. The Chinese medicine composition for oral cavity according to claim 1 or 2, characterized in that: The raw materials of the traditional Chinese medicine composition include, by weight, 0.05-1 part of bezoar, 0.3-4 parts of borneol, 3-20 parts of soapberry, 3-20 parts of toothbrush tree, 4-8 parts of honeysuckle and 0.4-2 parts of houttuynia cordata.

5. The Chinese medicine composition for oral cavity according to claim 1 or 2, characterized in that: The Chinese medicine composition comprises the following components: honeysuckle extract, houttuynia cordata extract, soapberry extract, toothbrush tree extract, bezoar powder and borneol powder; preferably, the honeysuckle extract, houttuynia cordata extract, soapberry extract and toothbrush tree extract are all water extracts.

6. The method for preparing the oral Chinese medicine composition according to any one of claims 1 to 5, characterized in that: The steps include: Preparation of soapberry extract and / or toothbrush tree extract: Take soapberry peel and / or toothbrush tree trunk, add water and reflux to extract 2 to 3 times, each time for 1.5 to 3 hours, the solvent ratio is 1:5 to 1:10, combine the extracts of each extraction, separate the solid and liquid, collect the liquid phase and concentrate it to a relative density of 1.05 to 1.10, and obtain; Preparation of honeysuckle extract and / or houttuynia cordata extract: take honeysuckle and / or houttuynia cordata, add water and reflux to extract 1 to 2 times, each time for 0.5 to 1 hour, the solvent ratio is 1:15 to 1:20, the extracts from each extraction are combined, the solid-liquid separation is performed, and the liquid phase is collected and concentrated to a relative density of 1.05 to 1.10 to obtain; Preparation of bezoar powder and / or borneol powder: crush bezoar and / or borneol powder in an environment below 28°C. Wherein, the toothbrush tree trunk includes at least one of a root, a branch or a stem.

7. Use of the Chinese medicine composition according to any one of claims 1 to 5 in the preparation of oral care products.

8. A toothpaste, characterized in that: The toothpaste paste contains the Chinese medicine composition according to any one of claims 1 to 5 and a toothpaste base; preferably, the mass proportion of the Chinese medicine composition in the paste is 1 to 20%; more preferably, the mass proportion of the Chinese medicine composition in the paste is 5 to 15%.

9. The toothpaste according to claim 8, characterized in that: The toothpaste paste is made of the following raw material components in parts by weight: 1% to 20% of Chinese medicine additives, 25% to 65% of moisturizers, 0.4% to 1% of thickeners, 10% to 60% of abrasives, 1% to 2% of foaming agents, 0.5% to 2% of flavors, 0.1% to 0.5% of preservatives, 0.05% to 0.4% of sweeteners, 0.2% to 5% of stabilizers, and the balance is water; preferably The toothpaste paste is made of the following raw material components in parts by weight: 5% to 15% of traditional Chinese medicine additives, 25% to 65% of moisturizing agents, 0.4% to 1% of thickeners, 10% to 40% of abrasives, 1% to 2% of foaming agents, 0.5% to 2% of flavors, 0.1% to 0.5% of preservatives, 0.05% to 0.4% of sweeteners, 0.2% to 5% of stabilizers, and the balance is water.

10. A method for preparing toothpaste using the Chinese medicine composition according to any one of claims 1 to 5, characterized in that: The method comprises the following steps: mixing bezoar, borneol and abrasive to obtain a bezoar-borneol-abrasive mixture; mixing other ingredients in the traditional Chinese medicine composition with a moisturizer, a sweetener and a preservative, adding water, and then adding the bezoar-borneol-abrasive mixture, a thickener, a foaming agent and a stabilizer, mixing, grinding, degassing, adding essence, mixing, and grinding again to obtain the mixture; preferably, the bezoar and borneol mixture is first prepared into a powder and then added; in the traditional Chinese medicine composition, soapberry and toothbrush tree are mixed and extracted before adding, and honeysuckle and houttuynia cordata are mixed and extracted before adding.

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