Compound donkey-hide gelatin paste capable of replenishing and nourishing blood and preparation method thereof

Abstract

The invention provides compound donkey-hide gelatin paste capable of replenishing and nourishing blood and a preparation method thereof, and relates to the technical field of food processing. The preparation method comprises the following steps: firstly, cutting colla corii asini into small blocks, carrying out ultrasonic treatment by using a pretreatment solution containing organic acid, stewing, cooling and centrifuging to obtain a stewed colla corii asini solution; adding 4% of pepsase into the stewed donkey-hide gelatin solution for enzymolysis to obtain a donkey-hide gelatin enzymatic hydrolysate; diluting the donkey-hide gelatin enzymatic hydrolysate, and performing ultrafiltration to obtain donkey-hide gelatin polypeptide; finally, mixing the colla corii asini polypeptide with the compound traditional Chinese medicine extract, and concentrating to obtain the compound colla corii asini paste. The enzyme activity of the pepsin is limited to be 1 * 10 < 5 > U / g, the enzymolysis pH is 2, the enzymolysis temperature is 42 DEG C, the enzymolysis time is 1-2 hours, and the dosage of the enzyme is 0.3%-0.5% of the substrate. The compound colla corii asini paste is high in bioavailability and absorption efficiency, can keep good stability at normal temperature, is remarkably prolonged in shelf life, overcomes the problems in the traditional colla corii asini paste, and has a good application prospect.

Description

Technical Field

[0001] The invention relates to the technical field of food processing, and in particular to a blood-enriching and blood-nourishing composite donkey-hide gelatin paste and a preparation method thereof. Background Art

[0002] Donkey-hide gelatin is a gelatin block made from the skin of donkeys, an animal of the Equine family, which is bleached, soaked, and dehaired before being boiled. In ancient times, it was named after the Dong'e County in Shandong Province where it was produced. According to the "Shennong's Herbal Classic", donkey-hide gelatin "treats abdominal collapse, excessive labor and sweating, waist and abdominal pain, limb pain, bleeding in women, and stabilizing pregnancy", and is a good product for blood supplement.

[0003] Donkey-hide gelatin paste is made according to the prescription of traditional Chinese medicine. After repeatedly decocting the Chinese medicine, the residue is removed, and the juice is decocted and concentrated over a low fire. Donkey-hide gelatin is added to boil, and a thick semi-fluid oral preparation is made. In the Chinese medicine diet therapy formula, ingredients that are both medicinal and edible are also selected and made into solid pastry with donkey-hide gelatin for easy consumption. Donkey-hide gelatin paste mostly uses tonic, strengthening, and blood circulation drugs. It has an auxiliary therapeutic effect on the sick, and can enhance the physical fitness of healthy people, keep energetic, and reduce the occurrence of diseases. At the same time, donkey-hide gelatin paste combines traditional Chinese medicine and dietary technology with nutritional technology, and selects ingredients that are both medicinal and edible, such as classic diet therapy formulas, refined with walnut kernels, black sesame seeds and donkey-hide gelatin. It is easy to take and is deeply loved by people. Donkey-hide gelatin paste has the characteristics of small size and easy to carry, making it one of the most popular tonic foods.

[0004] However, although solid pastry-like donkey-hide gelatin paste is easy to carry and has a long shelf life, the production process is complicated and time-consuming, and it is not easy to dissolve. It needs to be chewed or soaked in water before eating. In addition, solid donkey-hide gelatin paste is easily affected by moisture, needs to be sealed and stored, and is not resistant to high temperatures. Because its ingredients are relatively greasy, people with weak spleen and stomach are at risk of "greasy and stomach-damaging" after eating it, and the absorption rate is low. The nourishing effect of paste-like donkey-hide gelatin paste is more obvious. It does not need to be decocted and can be eaten directly or added to warm water. However, the storage requirements of paste-like donkey-hide gelatin paste are high, it is easy to precipitate and deteriorate, and the stability is poor. It generally needs to be refrigerated. And compared with solid pastry-like donkey-hide gelatin paste, although the absorption rate of paste-like donkey-hide gelatin paste has increased, the degree of increase is very limited.

[0005] The invention patent with the publication number CN118556828A discloses a method for making donkey-hide gelatin paste containing sea cucumber particles, which relates to the field of food technology. Its technical points include the following steps: S1, obtaining sea cucumber particles by pretreatment of sea cucumbers, ripening of sea cucumbers, cutting and ultra-high pressure treatment of sea cucumber particles; S2, soaking the treated sea cucumber particles in a mixed solution of tea polyphenols, trehalose and sodium alginate to form a water-locking coat; S3, putting donkey-hide gelatin powder into a pot and adding yellow wine to dissolve, adding rock sugar to continue boiling, stirring until the rock sugar is completely melted and presents a flag-hanging state, then successively adding auxiliary materials and sea cucumber particles for mixing, and finally through molding, cooling, demoulding, cutting and packaging. The donkey-hide gelatin paste can not only visually see the sea cucumber particles, but also can retain the moisture of the sea cucumber to the greatest extent, effectively maintain the taste of the sea cucumber, and avoid the loss of water-soluble nutrients and functional components in the process of preparing donkey-hide gelatin paste. It can be seen that the method still prepares a solid pastry-like donkey-hide gelatin paste.

[0006] In summary, the two common donkey-hide gelatin pastes currently have their own advantages and disadvantages, and it is difficult for a product to have the advantages of both conventional donkey-hide gelatin pastes. Therefore, it is necessary to develop a new donkey-hide gelatin paste product that improves the product absorption rate while making it have good stability and a longer shelf life, so as to improve the blood-tonifying and blood-nourishing effect of donkey-hide gelatin paste. Summary of the invention

[0007] In order to solve the above problems, the present invention provides a composite donkey-hide gelatin paste and its preparation method. The present invention decomposes donkey-hide gelatin into polypeptides with a molecular weight of less than 5kD, and then uses the polypeptides as raw materials and mixes them with extracts of other Chinese medicine raw materials to obtain a composite donkey-hide gelatin paste with good blood-tonifying and nourishing effects.

[0008] The preparation method of the composite donkey-hide gelatin paste of the present invention comprises the following steps:

[0009] S1, cutting the donkey-hide gelatin into small pieces, treating it with a pretreatment solution containing an organic acid by ultrasonic treatment, stewing it, cooling it, and centrifuging it to obtain a stewed donkey-hide gelatin solution;

[0010] S2, adding pepsin to the donkey-hide gelatin stewed liquid for enzymolysis to obtain donkey-hide gelatin enzymolysis liquid;

[0011] S3, diluting the donkey-hide gelatin hydrolysate and then ultrafiltering to obtain donkey-hide gelatin polypeptide;

[0012] S4, mixing the donkey-hide gelatin polypeptide and the compound Chinese medicine extract and concentrating them to obtain a compound donkey-hide gelatin paste.

[0013] Furthermore, the organic acid is one or more of citric acid, malic acid, caffeic acid, and lactic acid. Preferably, the organic acid is citric acid or lactic acid.

[0014] Furthermore, the mass concentration of the organic acid in the pretreatment liquid is 20%-50%. Preferably, the mass concentration of the organic acid in the pretreatment liquid is 35%.

[0015] Furthermore, the stewing temperature is 60°C-70°C, and the stewing time is 20-40 minutes. Preferably, the stewing temperature is 65°C, and the stewing time is 30 minutes.

[0016] Furthermore, the enzymatic activity of the pepsin is 1×10 5 U / g, the enzymatic hydrolysis pH is 2, the enzymatic hydrolysis temperature is 42°C, the enzymatic hydrolysis time is 1-2h, and the amount of enzyme is 0.3%-0.5% of the substrate. Preferably, the enzymatic hydrolysis time is 1.5h, and the amount of enzyme is 0.4% of the substrate mass.

[0017] Furthermore, the dilution multiple of the donkey-hide gelatin enzymatic hydrolysate is 20-50 times.

[0018] Furthermore, the ultrafiltration temperature is 40°C and the ultrafiltration membrane molecular weight cutoff is 5kD.

[0019] Furthermore, the preparation method of the composite Chinese medicine extract is as follows: mix 150 parts by mass of ginseng, 100 parts by mass of Codonopsis pilosula, 100 parts by mass of Rehmannia glutinosa, and 50 parts by mass of hawthorn, soak them in water for 30-60 minutes, decoct the soaked medicinal materials three times, filter, let stand, combine the filtrate and concentrate to obtain the composite Chinese medicine extract.

[0020] Furthermore, the three decoction times are 2h, 2h, and 1h respectively. During each decoction, first boil over high heat and then switch to low heat.

[0021] Furthermore, the mass ratio of the donkey-hide gelatin polypeptide to the composite traditional Chinese medicine extract is 1:(5-8).

[0022] The present invention also provides a composite donkey-hide gelatin paste prepared according to the method.

[0023] Compared with the prior art, the beneficial technical effects of the present invention are:

[0024] The present invention significantly improves its bioavailability and absorption efficiency by decomposing donkey-hide gelatin into polypeptides with a molecular weight of less than 5kD, thereby enhancing the effect of nourishing blood. In addition, small molecular weight polypeptides have higher solubility and stability, are not prone to precipitation or decomposition, and can improve the storage stability of composite donkey-hide gelatin paste. In addition, the polypeptide prepared by the present invention shows good stability under different pH values ​​and temperature conditions, reducing the risk of product deterioration during storage and transportation. Small molecule polypeptides have good water solubility and dispersibility, can be evenly distributed in the product, avoid granularity, further enhance the mouthfeel of the product, and make it more delicate and smooth.

[0025] The present invention adopts organic acids such as citric acid for pretreatment and removes macromolecular impurities through ultrafiltration technology, thereby ensuring the purity and stability of donkey-hide gelatin polypeptide.

[0026] The present invention mixes donkey-hide gelatin polypeptide with Chinese medicinal extracts such as ginseng, codonopsis, rehmannia, hawthorn, etc., and utilizes the synergistic effect of each component to further enhance the comprehensive effects of enriching blood, nourishing blood and conditioning the body.

[0027] The invention improves production efficiency and product quality by optimizing process parameters such as stewing, enzymolysis and ultrafiltration, and ensures the stability and consistency of the composite donkey-hide gelatin paste.

[0028] The composite donkey-hide gelatin paste of the present invention not only has the effect of enriching and nourishing blood, but also has multiple health care functions such as enhancing immunity and resisting fatigue.

[0029] The composite donkey-hide gelatin paste of the present invention can also maintain good stability at room temperature, and the shelf life is significantly prolonged, thus overcoming the problems existing in traditional donkey-hide gelatin paste and having good application prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] The present invention will be further described below in conjunction with the accompanying drawings.

[0031] Figure 1 The present invention is a process flow chart of the preparation process of the composite donkey-hide gelatin paste. DETAILED DESCRIPTION

[0032] The present invention provides a method for preparing a composite donkey-hide gelatin paste, comprising the following steps:

[0033] S1, cutting the donkey-hide gelatin into small pieces, treating it with a pretreatment solution containing an organic acid by ultrasonic treatment, stewing it, cooling it, and centrifuging it to obtain a stewed donkey-hide gelatin solution;

[0034] S2, adding pepsin to the donkey-hide gelatin stewed liquid for enzymolysis to obtain donkey-hide gelatin enzymolysis liquid;

[0035] S3, diluting the donkey-hide gelatin hydrolysate and then ultrafiltering to obtain donkey-hide gelatin polypeptide;

[0036] S4, mixing the donkey-hide gelatin polypeptide and the compound Chinese medicine extract and concentrating them to obtain a compound donkey-hide gelatin paste.

[0037] In one embodiment, the organic acid is one or more of citric acid, malic acid, caffeic acid, and lactic acid.

[0038] In one embodiment, the organic acid is citric acid or lactic acid.

[0039] In one embodiment, the mass concentration of the organic acid in the pretreatment solution is 20%-50%.

[0040] In one embodiment, the mass concentration of the organic acid in the pretreatment solution is 35%.

[0041] The traditional method of processing donkey-hide gelatin is generally to melt it and obtain donkey-hide gelatin stewed liquid. However, due to the characteristics of donkey skin raw materials, donkey-hide gelatin stewed liquid is generally an opaque yellow-brown liquid, and turbid with an oil layer. Therefore, if the stewed liquid is to be further processed, it is necessary to pre-treat the stewed liquid to remove impurities such as grease in the stewed liquid. For example, pre-treatment methods such as organic solvents and macroporous resins. However, directly treating the stewed liquid will lead to the loss of effective ingredients. Therefore, the present invention abandons the traditional process of degreasing the stewed liquid, but directly treats the donkey-hide gelatin raw material. Through the pre-treatment liquid combined with the ultrasonic treatment process of the present invention, grease can be removed at the raw material stage, and then a relatively pure stewed liquid can be obtained after stewing, reducing the difficulty of subsequent processing. Finally, the treatment process can remove the fishy smell of donkey skin and improve the sensory quality of donkey-hide gelatin cake.

[0042] In one embodiment, the stewing temperature is 60° C.-70° C., and the stewing time is 20-40 minutes.

[0043] In one embodiment, the stewing temperature is 65° C. and the stewing time is 30 minutes.

[0044] In one embodiment, the enzymatic activity of pepsin is 1×10 5 U / g, the enzymatic hydrolysis pH is 2, the enzymatic hydrolysis temperature is 42°C, the enzymatic hydrolysis time is 1-2h, and the enzyme dosage is 0.3%-0.5% of the substrate mass.

[0045] In one embodiment, the enzymolysis time is 1.5 h, and the amount of enzyme used is 0.4% of the substrate.

[0046] Pepsin is a digestive protease secreted by the chief cells of the gastric mucosa, and its precursor is pepsinogen, which is activated to pepsin under the action of gastric acid. This enzyme has the highest activity in an acidic environment (optimum pH value is about 1.5-2.0), and mainly acts on the peptide bonds of aromatic amino acids and acidic amino acids in proteins. In the process of preparing the composite donkey-hide gelatin paste, the present invention utilizes pepsin to enzymolyze donkey-hide gelatin, and specific donkey-hide gelatin polypeptides can be obtained to improve the bioavailability of the composite donkey-hide gelatin paste. At the same time, the present invention strictly limits the type of enzyme and the specific process of enzymolysis to achieve the maximum acquisition of specific structural polypeptides.

[0047] In one embodiment, the dilution multiple of the donkey-hide gelatin enzymatic hydrolysate is 20-50 times.

[0048] In one embodiment, the ultrafiltration temperature is 40°C and the ultrafiltration membrane molecular weight cutoff is 5kD.

[0049] In one embodiment, the preparation method of the composite Chinese medicine extract is as follows: 150 parts by mass of ginseng, 100 parts by mass of Codonopsis pilosula, 100 parts by mass of Rehmannia glutinosa, and 50 parts by mass of hawthorn are mixed, soaked in water for 30-60 minutes, decocted the soaked medicinal materials three times, filtered, allowed to stand, and the filtrate was combined and concentrated to obtain the composite Chinese medicine extract.

[0050] In one embodiment, the three decoction times are 2h, 2h, and 1h respectively. During each decoction, the mixture is first boiled over high heat and then over low heat.

[0051] In one embodiment, the mass ratio of the donkey-hide gelatin polypeptide to the composite Chinese herbal medicine extract is 1:(5-8).

[0052] The present invention also provides a composite donkey-hide gelatin paste prepared according to the method.

[0053] The technical solution provided by the present invention is further described below in conjunction with embodiments.

[0054] The preparation method of the composite Chinese medicine extract used in the embodiments of the present invention and the comparative example is as follows: 150g of ginseng, 100g of Codonopsis pilosula, 100g of Rehmannia glutinosa, and 50g of hawthorn are mixed, soaked in water for 45min, and the soaked medicinal materials are decocted three times for 2h, 2h, and 1h respectively, filtered, allowed to stand, and the filtrate is combined and concentrated to a relative density of 1.1 to obtain.

[0055] Unless otherwise specified, all experiments were repeated three times. SPSS 21.0 was used for analysis of variance (ANOVA) and Duncan's multiple comparison analysis, and the results were expressed as mean ± SD.

[0056] Embodiment 1 A method for preparing a composite donkey-hide gelatin paste, comprising the following steps:

[0057] S1, cutting the donkey-hide gelatin into small pieces of 2 cm×2 cm×2 cm, treating the pretreatment solution containing 30 wt.% citric acid at 300 W ultrasonic wave for 15 min, then stewing at 65° C. for 30 min, cooling, and centrifuging to obtain stewed donkey-hide gelatin solution;

[0058] S2, add 1×10 5 U / g of pepsin, and then enzymolyze for 1.5h at 42°C and pH 2 to obtain donkey-hide gelatin enzymolysis solution, wherein the amount of enzyme used is 0.4% of the substrate mass;

[0059] S3, diluting the donkey-hide gelatin hydrolysate 20 times and preheating it in a water bath to 40°C, and passing it through an ultrafiltration membrane with a molecular weight cutoff of 5kD at a column pressure of 0.15MPa to obtain donkey-hide gelatin polypeptide;

[0060] S4. Mix the donkey-hide gelatin polypeptide and the composite Chinese medicine extract in a mass ratio of 1:6.5 and then concentrate to obtain a composite donkey-hide gelatin paste.

[0061] Embodiment 2 A method for preparing a composite donkey-hide gelatin paste, the steps are as follows:

[0062] S1, cut the donkey-hide gelatin into small pieces of 2cm×2cm×2cm, treat them with a pretreatment solution containing 30% citric acid at 300W ultrasonic for 15min, then stew them at 60℃ for 40min, cool them, and centrifuge them to obtain stewed donkey-hide gelatin liquid;

[0063] S2, add 1×10 5 U / g of pepsin, and then enzymolyze for 1.5h at 42°C and pH 2 to obtain donkey-hide gelatin enzymolysis solution, wherein the amount of enzyme used is 0.4% of the substrate mass;

[0064] S3, diluting the donkey-hide gelatin hydrolysate 20 times and preheating it in a water bath to 40°C, and passing it through an ultrafiltration membrane with a molecular weight cutoff of 5kD at a column pressure of 0.15MPa to obtain donkey-hide gelatin polypeptide;

[0065] S4. Mix the donkey-hide gelatin polypeptide and the composite Chinese medicine extract in a mass ratio of 1:6.5 and then concentrate to obtain a composite donkey-hide gelatin paste.

[0066] Embodiment 3 A method for preparing a composite donkey-hide gelatin paste, the steps are as follows:

[0067] S1, cut the donkey-hide gelatin into small pieces of 2cm×2cm×2cm, treat them with a pretreatment solution containing 30% citric acid at 300W ultrasonic for 15min, then stew them at 75℃ for 20min, cool them, and centrifuge them to obtain stewed donkey-hide gelatin liquid;

[0068] S2, add 1×10 5 U / g of pepsin, and then enzymolyze for 1.5h at 42°C and pH 2 to obtain donkey-hide gelatin enzymolysis solution, wherein the amount of enzyme used is 0.4% of the substrate mass;

[0069] S3, diluting the donkey-hide gelatin hydrolysate 20 times and preheating it in a water bath to 40°C, and passing it through an ultrafiltration membrane with a molecular weight cutoff of 5kD at a column pressure of 0.15MPa to obtain donkey-hide gelatin polypeptide;

[0070] S4. Mix the donkey-hide gelatin polypeptide and the composite Chinese medicine extract in a mass ratio of 1:6.5 and then concentrate to obtain a composite donkey-hide gelatin paste.

[0071] Comparative Example 1 A method for preparing a composite donkey-hide gelatin paste, the steps are as follows:

[0072] S1. Cut the donkey-hide gelatin into small pieces of 2 cm×2 cm×2 cm, treat with deionized water at 300W ultrasonic for 15 min, then stew at 60°C for 40 min, cool, and centrifuge to obtain stewed donkey-hide gelatin liquid;

[0073] S2, add 1×10 5 U / g of pepsin, and then enzymolyze for 1.5h at 42°C and pH 2 to obtain donkey-hide gelatin enzymolysis solution, wherein the amount of enzyme used is 0.4% of the substrate mass;

[0074] S3, diluting the donkey-hide gelatin hydrolysate 20 times and preheating it in a water bath to 40°C, and passing it through an ultrafiltration membrane with a molecular weight cutoff of 5kD at a column pressure of 0.15MPa to obtain donkey-hide gelatin polypeptide;

[0075] S4. Mix the donkey-hide gelatin polypeptide and the composite Chinese medicine extract in a mass ratio of 1:6.5 and then concentrate to obtain a composite donkey-hide gelatin paste.

[0076] Comparative Example 2 A method for preparing a composite donkey-hide gelatin paste, the steps are as follows:

[0077] S1. Cut the donkey-hide gelatin into small pieces of 2 cm×2 cm×2 cm, soak them in a pretreatment solution containing 30% citric acid for 15 min, then stew them at 60° C. for 40 min, cool them, and centrifuge them to obtain stewed donkey-hide gelatin solution;

[0078] S2, add 1×10 5 U / g of pepsin, and then enzymolyze for 1.5h at 42°C and pH 2 to obtain donkey-hide gelatin enzymolysis solution, wherein the amount of enzyme used is 0.4% of the substrate mass;

[0079] S3, diluting the donkey-hide gelatin hydrolysate 20 times and preheating it in a water bath to 40°C, and passing it through an ultrafiltration membrane with a molecular weight cutoff of 5kD at a column pressure of 0.15MPa to obtain donkey-hide gelatin polypeptide;

[0080] S4. Mix the donkey-hide gelatin polypeptide and the composite Chinese medicine extract in a mass ratio of 1:6.5 and then concentrate to obtain a composite donkey-hide gelatin paste.

[0081] Comparative Example 3 A method for preparing a composite donkey-hide gelatin paste, the steps are as follows:

[0082] S1, cut the donkey-hide gelatin into small pieces of 2cm×2cm×2cm, treat them with a pretreatment solution containing 30% acetic acid at 300W ultrasonic for 15min, then stew them at 65℃ for 30min, cool them, and centrifuge them to obtain stewed donkey-hide gelatin liquid;

[0083] S2, add 1×10 5U / g of pepsin, and then enzymolyze for 1.5h at 42°C and pH 2 to obtain donkey-hide gelatin enzymolysis solution, wherein the amount of enzyme used is 0.4% of the substrate mass;

[0084] S3, diluting the donkey-hide gelatin hydrolysate 20 times and preheating it in a water bath to 40°C, and passing it through an ultrafiltration membrane with a molecular weight cutoff of 5kD at a column pressure of 0.15MPa to obtain donkey-hide gelatin polypeptide;

[0085] S4. Mix the donkey-hide gelatin polypeptide and the composite Chinese medicine extract in a mass ratio of 1:6.5 and then concentrate to obtain a composite donkey-hide gelatin paste.

[0086] Comparative Example 4 A method for preparing a composite donkey-hide gelatin paste, the steps are as follows:

[0087] S1. Cut the donkey-hide gelatin into small pieces of 2 cm×2 cm×2 cm, treat with deionized water at 300W ultrasonic for 15 min, then stew at 60°C for 40 min, cool, and centrifuge to obtain stewed donkey-hide gelatin liquid;

[0088] S2, add 1×10 5 U / g of trypsin, and then enzymolyze for 2.5 hours at 42°C and pH 8 to obtain donkey-hide gelatin enzymolysis solution, wherein the amount of enzyme used is 0.4% of the substrate mass;

[0089] S3, diluting the donkey-hide gelatin hydrolysate 20 times and preheating it in a water bath to 40°C, and passing it through an ultrafiltration membrane with a molecular weight cutoff of 5kD at a column pressure of 0.15MPa to obtain donkey-hide gelatin polypeptide;

[0090] S4. Mix the donkey-hide gelatin polypeptide and the composite Chinese medicine extract in a mass ratio of 1:6.5 and then concentrate to obtain a composite donkey-hide gelatin paste.

[0091] Comparative Example 5 A method for preparing a composite donkey-hide gelatin paste, the steps are as follows:

[0092] S1, cut the donkey-hide gelatin into small pieces of 2cm×2cm×2cm, treat them with a pretreatment solution containing 30% citric acid at 300W ultrasonic for 15min, then stew them at 65℃ for 30min, cool them, and centrifuge them to obtain stewed donkey-hide gelatin liquid;

[0093] S2. After adjusting the stewed donkey-hide gelatin liquid to a relative density of 1.1, mix it with the compound Chinese herbal medicine extract at a mass ratio of 1:6.5 and then concentrate it to obtain the compound donkey-hide gelatin paste.

[0094] Comparative Example 6 A method for preparing a composite donkey-hide gelatin paste, the steps are as follows:

[0095] S1, cut the donkey-hide gelatin into small pieces of 2cm×2cm×2cm, treat them with a pretreatment solution containing 30% citric acid at 300W ultrasonic for 15min, then stew them at 65℃ for 30min, cool them, and centrifuge them to obtain stewed donkey-hide gelatin liquid;

[0096] S2, add 1×10 5 U / g of pepsin, and then enzymolyze for 1.5h at 42°C and pH 2 to obtain donkey-hide gelatin enzymolysis solution, wherein the amount of enzyme used is 0.4% of the substrate mass;

[0097] S3, diluting the donkey-hide gelatin hydrolysate 20 times and preheating it in a water bath to 40°C, and passing it through an ultrafiltration membrane with a molecular weight cutoff of 30kD at a column pressure of 0.15MPa to obtain donkey-hide gelatin polypeptide;

[0098] S4. Mix the donkey-hide gelatin polypeptide and the composite Chinese medicine extract in a mass ratio of 1:6.5 and then concentrate to obtain a composite donkey-hide gelatin paste.

[0099] Test Example 1 Blood Replenishment Effect Test

[0100] 110 male SPF Kunming mice weighing 18-20 g were used and adaptively fed for 7 days and randomly divided into 11 groups, 10 mice in each group, namely a normal group, a model group, Example 1-3 groups, and Comparative Example 1-6 groups.

[0101] Except for the normal group, the other groups were injected intraperitoneally with 10 mg·kg-1 busulfan solution on the 1st, 3rd, 5th, 7th, 9th, and 11th days; and injected intraperitoneally with 20 mg·kg-1 on the 2nd, 4th, 6th, 8th, 10th, and 12th days. -1 Cyclophosphamide was used to prepare aplastic anemia mouse model. Each group was intragastrically administered with a set dose (20 mL kg -1 ), once a day for 30 consecutive days, and the normal group and model group were given an equal amount of distilled water. After the last administration, the subjects were fasted but not watered for 12 hours, and then samples were taken for determination of hemoglobin (Hb) content, red blood cell (RBC) and white blood cell (WBC) counts. The test results are shown in Table 1.

[0102] Table 1

[0103] deal with <![CDATA[RBC(×10 12 ·L -1 )]]> <![CDATA[WBC(×10 9 ·L -1 )]]> <![CDATA[Hb(g·L -1 )]]> Normal group 9.42±1.58 4.91±1.78 133.02±2.48 Model Group 4.90±1.24 2.07±1.45 78.33±2.35 Example 1 8.69±1.16a 4.35±1.24a 120.78±1.94a Example 2 8.65±2.92a 4.35±1.31a 120.96±3.23a Example 3 8.66±1.64a 4.37±0.83a 121.04±2.11a Comparative Example 1 7.70±2.53c 3.74±1.26bc 110.17±2.56c Comparative Example 2 7.91±2.72b 3.85±1.45b 112.29±1.65b Comparative Example 3 7.78±1.41c 3.57±1.32c 111.02±3.77c Comparative Example 4 6.77±1.29d 3.18±0.21d 99.56±3.55d Comparative Example 5 6.31±2.37f 2.91±1.44e 94.46±2.22f Comparative Example 6 6.58±1.65e 3.01±1.76d 97.43±1.14e

[0104] Analysis of the test data revealed that the Hb content, RBC and WBC counts of the model group samples were significantly different from those of the normal group (P<0.05), while the Hb content, RBC and WBC counts of the samples of Examples 1-3 and Comparative Examples 1-5 were significantly different from those of the model group (P<0.05).

[0105] Test Example 2 Sensory and Stability Test

[0106] The generation of fishy smell of the products of Examples 1-3 and Comparative Examples 1-6 was investigated, and then each group of products was placed in a container of the same specifications, sealed and stored at room temperature and away from light for 3 months, and the precipitation and deterioration of the products were observed. The results are as follows:

[0107] project Fishy smell Sedimentation Deterioration Example 1 No fishy smell No precipitation Not spoiled Example 2 No fishy smell No precipitation Not spoiled Example 3 No fishy smell No precipitation Not spoiled Comparative Example 1 More obvious fishy smell A small amount of precipitation Not spoiled Comparative Example 2 No obvious fishy smell Microprecipitation Not spoiled Comparative Example 3 No obvious fishy smell Microprecipitation Not spoiled Comparative Example 4 No fishy smell More precipitation Deterioration Comparative Example 5 Obvious fishy smell Large amount of precipitation Deterioration Comparative Example 6 No fishy smell More precipitation Deterioration

[0108] Embodiment 4 A method for preparing a composite donkey-hide gelatin paste, the steps are as follows:

[0109] S1, cut the donkey-hide gelatin into small pieces of 2cm×2cm×2cm, treat them with a pretreatment solution containing 20% ​​citric acid at 300W ultrasonic for 15min, then stew them at 65℃ for 30min, cool them, and centrifuge them to obtain stewed donkey-hide gelatin liquid;

[0110] S2, add 1×10 5 U / g of pepsin, and then enzymolyze for 1.5h at 42°C and pH 2 to obtain donkey-hide gelatin enzymolysis solution, wherein the amount of enzyme used is 0.4% of the substrate mass;

[0111] S3, diluting the donkey-hide gelatin hydrolysate 20 times and preheating it in a water bath to 40°C, and passing it through an ultrafiltration membrane with a molecular weight cutoff of 5kD at a column pressure of 0.15MPa to obtain donkey-hide gelatin polypeptide;

[0112] S4. Mix the donkey-hide gelatin polypeptide and the composite Chinese medicine extract in a mass ratio of 1:6.5 and then concentrate to obtain a composite donkey-hide gelatin paste.

[0113] Embodiment 5 A method for preparing a composite donkey-hide gelatin paste, the steps are as follows:

[0114] S1, cut the donkey-hide gelatin into small pieces of 2 cm×2 cm×2 cm, treat them with a pretreatment solution containing 50% citric acid at 300W ultrasonic for 15 min, then stew them at 65°C for 30 min, cool them, and centrifuge them to obtain stewed donkey-hide gelatin liquid;

[0115] S2, add 1×10 5 U / g of pepsin, and then enzymolyze for 1.5h at 42°C and pH 2 to obtain donkey-hide gelatin enzymolysis solution, wherein the amount of enzyme used is 0.4% of the substrate mass;

[0116] S3, diluting the donkey-hide gelatin hydrolysate 20 times and preheating it in a water bath to 40°C, and passing it through an ultrafiltration membrane with a molecular weight cutoff of 5kD at a column pressure of 0.15MPa to obtain donkey-hide gelatin polypeptide;

[0117] S4. Mix the donkey-hide gelatin polypeptide and the composite Chinese medicine extract in a mass ratio of 1:6.5 and then concentrate to obtain a composite donkey-hide gelatin paste.

[0118] Embodiment 6 A method for preparing a composite donkey-hide gelatin paste, the steps are as follows:

[0119] S1, cut the donkey-hide gelatin into small pieces of 2cm×2cm×2cm, treat them with a pretreatment solution containing 30% citric acid at 300W ultrasonic for 15min, then stew them at 65℃ for 30min, cool them, and centrifuge them to obtain stewed donkey-hide gelatin liquid;

[0120] S2, add 1×10 5 U / g of pepsin, and then enzymolyze for 1.5h at 42°C and pH 2 to obtain donkey-hide gelatin enzymolysis solution, wherein the amount of enzyme used is 0.4% of the substrate mass;

[0121] S3, diluting the donkey-hide gelatin hydrolysate 50 times and preheating it in a water bath to 40°C, and passing it through an ultrafiltration membrane with a molecular weight cutoff of 5kD at a column pressure of 0.15MPa to obtain donkey-hide gelatin polypeptide;

[0122] S4. Mix the donkey-hide gelatin polypeptide and the composite Chinese medicine extract in a mass ratio of 1:6.5 and then concentrate to obtain a composite donkey-hide gelatin paste.

[0123] Embodiment 7 A method for preparing a composite donkey-hide gelatin paste, the steps are as follows:

[0124] S1, cut the donkey-hide gelatin into small pieces of 2cm×2cm×2cm, treat them with a pretreatment solution containing 30% citric acid at 300W ultrasonic for 15min, then stew them at 65℃ for 30min, cool them, and centrifuge them to obtain stewed donkey-hide gelatin liquid;

[0125] S2, add 1×10 5 U / g of pepsin, and then enzymolyze for 1.5h at 42°C and pH 2 to obtain donkey-hide gelatin enzymolysis solution, wherein the amount of enzyme used is 0.4% of the substrate mass;

[0126] S3, diluting the donkey-hide gelatin hydrolysate 20 times and preheating it in a water bath to 40°C, and passing it through an ultrafiltration membrane with a molecular weight cutoff of 5kD at a column pressure of 0.15MPa to obtain donkey-hide gelatin polypeptide;

[0127] S4. Mix the donkey-hide gelatin polypeptide and the composite Chinese medicine extract in a mass ratio of 1:5 and then concentrate to obtain a composite donkey-hide gelatin paste.

[0128] Embodiment 8 A method for preparing a composite donkey-hide gelatin paste, the steps are as follows:

[0129] S1, cut the donkey-hide gelatin into small pieces of 2cm×2cm×2cm, treat them with a pretreatment solution containing 30% citric acid at 300W ultrasonic for 15min, then stew them at 65℃ for 30min, cool them, and centrifuge them to obtain stewed donkey-hide gelatin liquid;

[0130] S2, add 1×10 5 U / g of pepsin, and then enzymolyze for 1.5h at 42°C and pH 2 to obtain donkey-hide gelatin enzymolysis solution, wherein the amount of enzyme used is 0.4% of the substrate mass;

[0131] S3, diluting the donkey-hide gelatin hydrolysate 20 times and preheating it in a water bath to 40°C, and passing it through an ultrafiltration membrane with a molecular weight cutoff of 5kD at a column pressure of 0.15MPa to obtain donkey-hide gelatin polypeptide;

[0132] S4. Mix the donkey-hide gelatin polypeptide and the compound Chinese medicine extract in a mass ratio of 1:8 and then concentrate to obtain a compound donkey-hide gelatin paste.

[0133] Embodiment 9 A method for preparing a composite donkey-hide gelatin paste, comprising the following steps:

[0134] S1, cut the donkey-hide gelatin into small pieces of 2 cm×2 cm×2 cm, treat them with a pretreatment solution containing 30% lactic acid at 300W ultrasonic for 15 min, then stew them at 65°C for 30 min, cool them, and centrifuge them to obtain stewed donkey-hide gelatin liquid;

[0135] S2, add 1×10 5 U / g of pepsin, and then enzymolyze for 1.5h at 42°C and pH 2 to obtain donkey-hide gelatin enzymolysis solution, wherein the amount of enzyme used is 0.4% of the substrate mass;

[0136] S3, diluting the donkey-hide gelatin hydrolysate 20 times and preheating it in a water bath to 40°C, and passing it through an ultrafiltration membrane with a molecular weight cutoff of 5kD at a column pressure of 0.15MPa to obtain donkey-hide gelatin polypeptide;

[0137] S4. Mix the donkey-hide gelatin polypeptide and the composite Chinese medicine extract in a mass ratio of 1:6.5 and then concentrate to obtain a composite donkey-hide gelatin paste.

[0138] Embodiment 10 A method for preparing a composite donkey-hide gelatin paste, comprising the following steps:

[0139] S1, cut the donkey-hide gelatin into small pieces of 2cm×2cm×2cm, treat them with a pretreatment solution containing 30% citric acid at 400W ultrasonic for 15min, then stew them at 65℃ for 30min, cool them, and centrifuge them to obtain stewed donkey-hide gelatin liquid;

[0140] S2, add 1×10 5 U / g of pepsin, and then enzymolyze for 1.5h at 42°C and pH 2 to obtain donkey-hide gelatin enzymolysis solution, wherein the amount of enzyme used is 0.3% of the substrate mass;

[0141] S3, diluting the donkey-hide gelatin hydrolysate 20 times and preheating it in a water bath to 40°C, and passing it through an ultrafiltration membrane with a molecular weight cutoff of 5kD at a column pressure of 0.15MPa to obtain donkey-hide gelatin polypeptide;

[0142] S4. Mix the donkey-hide gelatin polypeptide and the composite Chinese medicine extract in a mass ratio of 1:6.5 and then concentrate to obtain a composite donkey-hide gelatin paste.

[0143] Embodiment 11 A method for preparing a composite donkey-hide gelatin paste, comprising the following steps:

[0144] S1, cut the donkey-hide gelatin into small pieces of 2cm×2cm×2cm, treat them with a pretreatment solution containing 30% citric acid at 500W ultrasonic for 10min, then stew them at 65°C for 30min, cool them, and centrifuge them to obtain stewed donkey-hide gelatin liquid;

[0145] S2, add 1×10 5 U / g of pepsin, and then enzymolyze for 1.5h at 42°C and pH 2 to obtain donkey-hide gelatin enzymolysis solution, wherein the amount of enzyme used is 0.5% of the substrate mass;

[0146] S3, diluting the donkey-hide gelatin hydrolysate 20 times and preheating it in a water bath to 40°C, and passing it through an ultrafiltration membrane with a molecular weight cutoff of 5kD at a column pressure of 0.15MPa to obtain donkey-hide gelatin polypeptide;

[0147] S4. Mix the donkey-hide gelatin polypeptide and the composite Chinese medicine extract in a mass ratio of 1:6.5 and then concentrate to obtain a composite donkey-hide gelatin paste.

[0148] After testing, the effects of the composite donkey-hide gelatin paste obtained in Examples 4-11 were not significantly different from those in Examples 1-3.

[0149] The principles and implementation methods of the present invention are described in this article using specific examples. The description of the above embodiments is only used to help understand the method and core idea of ​​the present invention. At the same time, for those skilled in the art, according to the idea of ​​the present invention, there will be changes in the specific implementation methods and application scope. In summary, the content of this specification should not be understood as limiting the present invention.

Claims

1. A method for preparing a composite donkey-hide gelatin paste, characterized in that: The following steps are involved: S1, cutting the donkey-hide gelatin into small pieces, treating it with a pretreatment solution containing an organic acid by ultrasonic treatment, stewing it, cooling it, and centrifuging it to obtain a stewed donkey-hide gelatin solution; S2, adding pepsin to the donkey-hide gelatin stewed liquid for enzymolysis to obtain donkey-hide gelatin enzymolysis liquid; S3, diluting the donkey-hide gelatin hydrolysate and then ultrafiltering to obtain donkey-hide gelatin polypeptide; S4, mixing the donkey-hide gelatin polypeptide and the composite Chinese medicine extract and concentrating them to obtain a composite donkey-hide gelatin paste; The enzymatic activity of pepsin is 1×10 5 U / g, the enzymatic hydrolysis pH is 2, the enzymatic hydrolysis temperature is 42°C, the enzymatic hydrolysis time is 1-2h, and the enzyme dosage is 0.3%-0.5% of the substrate mass.

2. The preparation method according to claim 1, characterized in that: The organic acid is one or more of citric acid, malic acid, caffeic acid and lactic acid.

3. The preparation method according to claim 1, characterized in that: The mass concentration of the organic acid in the pretreatment solution is 20%-50%.

4. The preparation method according to claim 1, characterized in that: The stewing temperature is 60° C.-70° C., and the stewing time is 20-40 minutes.

5. The preparation method according to claim 1, characterized in that: The enzymolysis time is 1.5 h, and the amount of enzyme used is 0.4% of the substrate mass.

6. The preparation method according to claim 1, characterized in that: The dilution multiple of the donkey-hide gelatin enzymatic hydrolysate is 20-50 times.

7. The preparation method according to claim 1, characterized in that: The ultrafiltration temperature is 40° C., and the ultrafiltration membrane molecular weight cutoff is 5 kD.

8. The preparation method according to claim 1, characterized in that: The preparation method of the composite Chinese medicine extract is as follows: 150 parts by weight of ginseng, 100 parts by weight of codonopsis pilosula, 100 parts by weight of rehmannia glutinosa and 50 parts by weight of hawthorn are mixed, soaked in water for 30-60 minutes, decocted the soaked medicinal materials three times, filtered, allowed to stand, combined the filtrate and concentrated to obtain the composite Chinese medicine extract.

9. The preparation method according to claim 1, characterized in that: The mass ratio of the donkey-hide gelatin polypeptide to the composite traditional Chinese medicine extract is 1:(5-8).

10. A composite donkey-hide gelatin paste, characterized in that: Prepared by the method according to any one of claims 1 to 9.

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