Reagent for promoting rapid germination of seed konjak as well as preparation device and preparation method of reagent

Abstract

The invention belongs to the technical field of konjak planting, and particularly relates to a reagent for promoting rapid germination of seed konjak and a preparation device of the reagent. The reagent formula for promoting the rapid germination of the seed konjak provided by the invention comprises 1-2mg / L of GA3, 40-60mg / L of KT, 10-20mg / L of NAA and an enzyme preparation, and a solvent is deionized water. Wherein the enzyme preparation is alpha-amylase with the activity of 25-40 u / mL, the pH value of the reagent is 5.5-6.8, and the osmotic pressure is 50-500 mOsm / kg. According to the reagent, the germination rate of the konjac verrucosa seed tubers is remarkably increased, and the germination time of the konjac verrucosa seed tubers is shortened. The invention provides a preparation device of a reagent for promoting rapid germination of seed konjak. The preparation device comprises a kettle body, and an auxin adjusting tank, an enzyme preparation adjusting tank, a mineral substance adjusting tank, a pH adjusting mechanism and an osmotic pressure adjusting mechanism which are connected with the kettle body through a liquid guide mechanism. The device can accurately control the preparation process of the reagent, ensure the proportion and characteristics of components, and ensure the consistency of the quality of the reagent.

Description

Technical Field

[0001] The invention belongs to the technical field of konjac planting, and particularly relates to a reagent for promoting rapid germination of konjac seed taro and a preparation device and a preparation method thereof. Background Art

[0002] Konjac is a high-value crop for both medicine and food, and is widely used in food, medicine, and chemical industries. However, the cultivation of konjac faces many challenges, including low germination rate, long growth cycle, and many diseases and pests. Traditional planting methods often rely on natural conditions, with unstable germination rates and slow growth rates, which seriously affect yield and quality. Therefore, developing efficient planting techniques and methods to improve the germination rate and growth rate of seed taro has become the focus of current research. Summary of the invention

[0003] The invention overcomes the shortcomings of the prior art and provides a reagent for promoting rapid germination of konjac seed taro and a preparation device and a preparation method thereof.

[0004] To achieve the above object, the technical solution adopted by the present invention is: a reagent for promoting rapid germination of konjac seeds, the formula of which is: 1-2 mg / L GA3, 40-60 mg / L KT, 10-20 mg / L NAA and enzyme preparation, and the solvent is deionized water.

[0005] In a preferred embodiment of the present invention, the enzyme preparation is one of α-amylase, β-amylase, glucoamylase and amyloglucosidase.

[0006] In a preferred embodiment of the present invention, the activity of the enzyme preparation is 25-40u / mL.

[0007] In a preferred embodiment of the present invention, the pH value of the reagent is 5.5-6.8.

[0008] In a preferred embodiment of the present invention, the osmotic pressure of the reagent is 50-500 mOsm / kg.

[0009] In a preferred embodiment of the present invention, the reagent also includes one or more of: 15-25 mg / L ammonium nitrate, 20-25 mg / L potassium nitrate, 30-45 mg / L magnesium sulfate and 30-40 mg / L superphosphate.

[0010] The invention also provides a device for preparing a reagent for promoting rapid germination of konjac seed taro, comprising a kettle body, an auxin regulating pool, an enzyme preparation regulating pool, a mineral regulating pool and a plurality of liquid guiding mechanisms; a plurality of medium inlets are arranged on the top of the kettle body, and a liquid outlet valve is arranged on the bottom; the kettle body is respectively connected with the auxin regulating pool, the enzyme preparation regulating pool and the mineral regulating pool through the liquid guiding mechanisms; the liquid guiding mechanism comprises an elastic hose, a gland arranged on the elastic hose, a roller arranged in the gland, a plurality of rollers arranged on the roller and a driver for providing rotational power to the roller.

[0011] In a preferred embodiment of the present invention, the device also includes a pH adjustment mechanism; the pH adjustment mechanism includes a pH monitor and a pH adjustment tank; the detection end of the pH monitor is inserted into the kettle body to monitor the pH value of the mixed solution in the kettle body; the pH adjustment tank includes an acidic tank and an alkaline tank, and the acidic tank and the alkaline tank are both connected to the kettle body through a liquid guiding mechanism.

[0012] In a preferred embodiment of the present invention, the device also includes an osmotic pressure regulating mechanism; the osmotic pressure regulating mechanism includes an osmotic pressure monitor and an osmotic pressure regulating tank; the detection end of the osmotic pressure monitor is inserted into the kettle body to monitor the pH value of the mixed solution in the kettle body; the osmotic pressure regulating tank includes a hypertonic solution tank and a hypotonic solution tank, and the hypertonic solution tank and the hypotonic solution tank are both connected to the kettle body through the liquid guiding mechanism.

[0013] In a preferred embodiment of the present invention, a magnetic stirring generator is further provided at the bottom of the kettle body; the stirring end of the magnetic stirring generator extends into the interior of the kettle body.

[0014] In a preferred embodiment of the present invention, the device also includes a control unit, which is signal-connected to each driver and the magnetic stirring generator to control the flow rate of liquid through the liquid guiding mechanism and the operating state of the magnetic stirring generator.

[0015] In a preferred embodiment of the present invention, a jacket is provided on the periphery of the kettle body; the interior of the jacket is hollow, and a heat transfer medium inlet and a heat transfer medium outlet are provided on both sides of the jacket.

[0016] In a preferred embodiment of the present invention, a sampling valve is also provided at the bottom of the kettle body for taking out a solution sample from the kettle body.

[0017] In a preferred embodiment of the present invention, a window is also provided on the top of the kettle body for observing the internal conditions of the kettle body.

[0018] The present invention also provides a method for preparing a reagent for promoting rapid germination of konjac seed taro, comprising the following steps:

[0019] S1, mixing GA3, KT and NAA with deionized water to obtain an auxin solution;

[0020] S2, adding an enzyme preparation to the auxin solution to obtain an active reagent;

[0021] S3, adding mineral solution to the active agent;

[0022] S4, adjusting the pH value and osmotic pressure of the active reagent in S3 to obtain a reagent for promoting rapid germination of konjac seed taro;

[0023] The concentrations of GA3, KT and NAA in the reagent for promoting rapid germination of konjac seeds are 1-2 mg / L, 40-60 mg / L and 10-20 mg / L respectively; the enzyme preparation is alpha-amylase with an activity of 25-40 u / mL; the mineral solution is a mixed solution of one or more of 15-25 mg / L ammonium nitrate, 20-25 mg / L potassium nitrate, 30-45 mg / L magnesium sulfate and 30-40 mg / L superphosphate; the pH value is 5.5-6.8, and the osmotic pressure is 50-500 mOsm / kg.

[0024] The present invention solves the defects existing in the background technology and has the following beneficial effects:

[0025] (1) The present invention provides a reagent for promoting rapid germination of konjac seed taro and a preparation device and method thereof, which solves the problem that the reagent for promoting rapid germination of konjac seed taro in the prior art is not suitable for germination of konjac seed taro, improves the germination rate of konjac seed taro and shortens its germination time, thereby improving economic benefits.

[0026] (2) The present invention provides a reagent formula for promoting rapid germination of konjac seed taro, which is 1-2mg / LGA3, 40-60mg / LKT, 10-20mg / LNAA and enzyme preparation, and the solvent is ionized water. The enzyme preparation is an α-amylase with an activity of 25-40u / mL, the pH value of the reagent is 5.5-6.8, and the osmotic pressure is 50-500mOsm / kg. The reagent of the present invention significantly improves the germination rate of the konjac seed taro and shortens its germination time. The experimental results show that the germination rate of the experimental group with the addition of α-amylase is more than 5% higher than that of the control group without the addition of α-amylase, and the germination time is shortened by about 1 day. This is because the germination of the konjac seed taro requires a lot of energy, and its starch content is high, and α-amylase can quickly decompose starch to provide sufficient energy for germination. The reagents for promoting rapid germination of konjac seed taro in the prior art are targeted at konjac varieties containing polysaccharides, and are not suitable for the germination of konjac seed taro. The reagent of the present invention adds alpha-amylase to the konjac seed taro in view of the high starch content of konjac seed taro, thereby effectively solving the problems of low germination rate and long germination time of konjac seed taro.

[0027] (3) The present invention provides a reagent for promoting rapid germination of konjac seed taro, and further adds one or more of 15-25mg / L ammonium nitrate, 20-25mg / L potassium nitrate, 30-45mg / L magnesium sulfate and 30-40mg / L superphosphate. The experimental results show that the growth rate of the experimental group with added auxin and minerals is more than 20% higher than that of the experimental group without added auxin and minerals, and the mass growth rate is more than 15%, which significantly improves the growth rate and mass growth rate of the konjac seed taro. The reagents for promoting rapid germination of konjac seed taro in the prior art focus on the germination stage and ignore the germination stage, resulting in low yield of the konjac seed taro. The reagent of the present invention effectively improves the growth rate and yield of the konjac seed taro, and improves the economic benefit.

[0028] (4) In the reagent of the present invention, the combined action of α-amylase, auxin and minerals effectively promotes the germination and growth of the Amorphophallus konjac seed taro. α-amylase provides energy and carbon source for germination, auxin and minerals promote growth, and the two cooperate with each other to achieve the catalysis of the rapid germination and growth of the Amorphophallus konjac seed taro, effectively improving the germination rate and growth rate of the Amorphophallus konjac seed taro.

[0029] (5) The present invention also provides a kind of reagent preparation device for promoting the rapid germination of konjak seed taro, which is provided with a kettle and an auxin regulating pool, an enzyme preparation regulating pool, and a mineral regulating pool connected to the kettle for storing various reagents. By arranging multiple regulating pools, different types of reagents are stored separately, and proportioning is performed according to actual needs to prepare a reagent for promoting the rapid germination of konjak seed taro. The device is also provided with a pH regulating mechanism, an osmotic pressure regulating mechanism, and a jacket sleeved on the kettle, which can monitor and adjust the pH value, osmotic pressure, and temperature of the mixed solution inside the kettle in real time. The device controls the rotation of the roller by a driver, thereby controlling the roller to extrude and release the elastic hose, and finally accurately controls the liquid circulation in the elastic hose, realizes flow control, accurately controls the proportion of each component of the reagent, and makes the konjak seed taro rapid germination reagent of the output have quality consistency, and finally prepares a reagent for promoting the rapid germination of konjak seed taro that meets the requirements. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art are briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in the present invention. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative work.

[0031] Figure 1 The present invention is a schematic diagram of the structure of a device for preparing a reagent for promoting rapid germination of konjac seed taro;

[0032] Figure 2It is a front view of the liquid guiding mechanism;

[0033] Figure 3 is a side view of the fluid guiding mechanism;

[0034] Figure 4 The present invention is a flow chart of a method for preparing a reagent for promoting rapid germination of konjac seed taro;

[0035] In the figure: 1. kettle body; 2. auxin regulating tank; 3. enzyme preparation regulating tank; 4. mineral regulating tank; 5. liquid guiding mechanism; 6. pH regulating mechanism; 7. osmotic pressure regulating mechanism; 8. magnetic stirring generator; 11. medium inlet; 12. liquid outlet valve; 13. jacket; 14. sampling valve; 15. window; 51. elastic hose; 52. gland; 53. roller; 54. roller; 55. driver; 61. pH monitor; 62. pH regulating tank; 71. osmotic pressure monitor; 72. osmotic pressure regulating tank; 131. heat transfer medium inlet; 132. heat transfer medium outlet; 621. acidic tank; 622. alkaline tank; 721. hypertonic solution tank; 722. hypotonic solution tank. DETAILED DESCRIPTION

[0036] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0037] In the following description, many specific details are set forth to facilitate a full understanding of the present invention, but the present invention may also be implemented in other ways different from those described herein. Therefore, the protection scope of the present invention is not limited to the specific embodiments disclosed below.

[0038] In the description of the present application, it should be understood that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the scope of protection of the present application. In addition, the terms "first", "second", etc. are only used for descriptive purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, features defined as "first", "second", etc. may explicitly or implicitly include one or more of the features. In the description of the invention, unless otherwise specified, "multiple" means two or more.

[0039] In the description of this application, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood by specific circumstances.

[0040] Application Overview:

[0041] Konjac mainly contains polysaccharides (such as glucomannan), proteins, amino acids, minerals, etc. Different varieties of konjac may have different contents of these ingredients. For example, the white konjac has a higher content of glucomannan and a lower content of starch; while the warty konjac has a very low content of glucomannan and a starch content of up to 77%.

[0042] Due to its high starch content and low glucomannan content, the nutritional content of Konjac is inferior to that of Konjac varieties with high glucomannan content. It has a weaker ability to resist soft rot in hot seasons and a lower growth coefficient.

[0043] However, most of the reagents for promoting rapid germination of konjac seed taro in the prior art are targeted at konjac varieties containing polysaccharides, and are not suitable for the germination of Amorphophallus konjac seed taro.

[0044] In summary, the high starch content and low glucomannan content of Amorphophallus vulgaris pose challenges to its germination and subsequent growth. This application provides a new idea on how to solve the germination problem of Amorphophallus vulgaris.

[0045] In the present invention: GA3 is gibberellin, which is a hormone that promotes plant growth and can promote seed germination, stem elongation, fruit development, etc.; KT is kinetin, which is a cytokinin that can promote cell division, delay leaf senescence, and promote lateral bud development; NAA is naphthaleneacetic acid, which is an auxin plant growth regulator that can promote root development, inhibit lateral bud growth, and promote fruit development.

[0046] Exemplary Reagents:

[0047] A reagent for promoting rapid germination of konjac seed taro, the formula of which is: 1-2 mg / L GA3, 40-60 mg / L KT, 10-20 mg / L NAA and enzyme preparation, and the solvent is deionized water.

[0048] The enzyme preparation is one of α-amylase, β-amylase, glucoamylase and amyloglucosidase, and the activity is 25-40u / mL.

[0049] The pH value of the reagent is 5.5-6.8 and the osmotic pressure is 50-500mOsm / kg.

[0050] The method for adjusting the pH value is: when the pH value of the reagent is greater than 6.8, 5%-10% citric acid solution is added to the reagent; when the pH value of the reagent is less than 5.5, 3%-8% sodium bicarbonate solution is added to the reagent.

[0051] The method for adjusting the osmotic pressure is: when the osmotic pressure of the reagent is less than 50mOsm / kg, 5%-10% potassium chloride solution is added to the reagent; when the osmotic pressure of the reagent is greater than 500mOsm / kg, water is added to the reagent.

[0052] In a specific embodiment, the reagent also includes one or more of 15-25 mg / L ammonium nitrate, 20-25 mg / L potassium nitrate, 30-45 mg / L magnesium sulfate and 30-40 mg / L superphosphate.

[0053] Example 1

[0054] A reagent for promoting rapid germination of konjac seed taro, the formula of which is 1 mg / L GA3, 40 mg / L KT and 10 mg / L NAA, the solvent is deionized water, the pH value of the reagent is 6.0, and the osmotic pressure is 300 mOsm / kg.

[0055] Example 2

[0056] A reagent for promoting rapid germination of konjac seed taro, the formula of which is 1 mg / L GA3, 40 mg / L KT, 10 mg / L NAA and 25 u / mL alpha-amylase, the solvent is deionized water, the pH value of the reagent is 6.0, and the osmotic pressure is 300 mOsm / kg.

[0057] Example 3

[0058] A reagent for promoting rapid germination of konjac seed taro, the formula of which is 1 mg / L GA3, 40 mg / L KT, 10 mg / L NAA and 27.5 u / mL alpha-amylase, the solvent is deionized water, the pH value of the reagent is 6.0, and the osmotic pressure is 300 mOsm / kg.

[0059] Example 4

[0060] A reagent for promoting rapid germination of konjac seed taro, the formula of which is 1 mg / L GA3, 40 mg / L KT, 10 mg / L NAA and 30 u / mL alpha-amylase, the solvent is deionized water, the pH value of the reagent is 6.0, and the osmotic pressure is 300 mOsm / kg.

[0061] Example 5

[0062] A reagent for promoting rapid germination of konjac seed taro, the formula of which is 1 mg / L GA3, 40 mg / L KT, 10 mg / L NAA and 32.5 u / mL alpha-amylase, the solvent is deionized water, the pH value of the reagent is 6.0, and the osmotic pressure is 300 mOsm / kg.

[0063] Example 6

[0064] A reagent for promoting rapid germination of konjac seed taro, the formula of which is 1 mg / L GA3, 40 mg / L KT, 10 mg / L NAA and 35 u / mL alpha-amylase, the solvent is deionized water, the pH value of the reagent is 6.0, and the osmotic pressure is 300 mOsm / kg.

[0065] Example 7

[0066] A reagent for promoting rapid germination of konjac seed taro, the formula of which is 1 mg / L GA3, 40 mg / L KT, 10 mg / L NAA and 37.5 u / mL alpha-amylase, the solvent is deionized water, the pH value of the reagent is 6.0, and the osmotic pressure is 300 mOsm / kg.

[0067] Example 8

[0068] A reagent for promoting rapid germination of konjac seed taro, the formula of which is 1 mg / L GA3, 40 mg / L KT, 10 mg / L NAA and 40 u / mL alpha-amylase, the solvent is deionized water, the pH value of the reagent is 6.0, and the osmotic pressure is 300 mOsm / kg.

[0069] Example 9

[0070] A reagent for promoting rapid germination of konjac taro, the formula of which is 1 mg / L GA3, 40 mg / L KT, 10 mg / L NAA, 25 u / mL alpha-amylase, 25 mg / L potassium nitrate, 40 mg / L magnesium sulfate and 35 mg / L superphosphate, the solvent is deionized water, the pH value of the reagent is 6.0, and the osmotic pressure is 300 mOsm / kg.

[0071] Example 10

[0072] A reagent for promoting rapid germination of konjac taro, the formula of which is 1 mg / L GA3, 40 mg / L KT, 10 mg / L NAA, 30 u / mL alpha-amylase, 25 mg / L potassium nitrate, 40 mg / L magnesium sulfate and 35 mg / L superphosphate, the solvent is deionized water, the pH value of the reagent is 6.0, and the osmotic pressure is 300 mOsm / kg.

[0073] Embodiment 11

[0074] A reagent for promoting rapid germination of konjac taro, the formula of which is 1 mg / L GA3, 40 mg / L KT, 10 mg / L NAA, 35 u / mL alpha-amylase, 25 mg / L potassium nitrate, 40 mg / L magnesium sulfate and 35 mg / L superphosphate, the solvent is deionized water, the pH value of the reagent is 6.0, and the osmotic pressure is 300 mOsm / kg.

[0075] Example 12

[0076] A reagent for promoting rapid germination of konjac taro, the formula of which is 1 mg / L GA3, 40 mg / L KT, 10 mg / L NAA, 40 u / mL alpha-amylase, 25 mg / L potassium nitrate, 40 mg / L magnesium sulfate and 35 mg / L superphosphate, the solvent is deionized water, the pH value of the reagent is 6.0, and the osmotic pressure is 300 mOsm / kg.

[0077] Example Experiment:

[0078] Select Amorphophallus taro with a mass of 50-100g, a relatively regular shape, a smooth surface and no diseases or insect pests.

[0079] Gently wash the seed potatoes with clean water to remove the dirt and impurities on the surface, then soak the seed potatoes in 0.1% potassium permanganate solution for 10-15 minutes to kill the bacteria and fungi on the surface, rinse the seed potatoes thoroughly with clean water to remove the residual disinfectant, and dry them.

[0080] The seed taro of the above species was divided into 13 groups. The seed taro was graded according to its size and weight to ensure that the size and weight of each group of seed taro were similar. Experimental groups 1 to 12 corresponded to Examples 1 to 12, and experimental group 13 did not add any reagent and germinated naturally.

[0081] The seed taro of each experimental group is soaked in the reagent of the corresponding embodiment respectively, soaked for 12h, and the soaking process environment temperature is 25 DEG C. The konjac seed taro after soaking is taken out, rinsed with clean water, and residual reagent is removed. The konjac seed taro is put into the seedling tray to accelerate germination, and an appropriate amount of moist soil and culture medium are added to the seedling tray to ensure that the konjac seed taro is partially buried in the soil. The seedling tray is put into a constant temperature incubator, and the temperature is set at 20 DEG C, and the relative humidity is set at 75%, and the whole germination process lasts for 14 days.

[0082] The number of taro seeds that germinated in each experimental group was recorded every day, and the germination rate was calculated: germination rate = (number of taro seeds that germinated / total number of taro seeds) × 100%.

[0083] The germination time of the taro seeds in each experimental group was recorded, and the average germination time was calculated: average germination time = the sum of the germination time of all taro seeds / the number of taro seeds that germinated.

[0084] The height of the shoots in each experimental group was measured every other day, and the growth rate was calculated: growth rate = (final height - initial height) / time.

[0085] The mass of the seed taro in each experimental group was measured before and 14 days after the treatment, and the mass increase rate was calculated: mass increase rate = (final mass - initial mass) / initial mass × 100%.

[0086] The data obtained after 14 days of experiment are shown in Table 1:

[0087] Table 1 Germination data table

[0088]

[0089]

[0090] Through the above experiments and experimental results, we can conclude that:

[0091] (1) Experimental Group 1 and Experimental Group 13: The germination rate, germination time, growth rate and mass growth rate of Experimental Group 1 were better than those of Experimental Group 13. This is because the experimental group 1, which corresponds to Example 1, supplemented the konjac seed taro with growth hormones that are beneficial to the germination of konjac seed taro, such as GA3, KT and NAA, by soaking the reagent before germination, so that the germination rate and tender shoot growth of Experimental Group 1 were better than those of the untreated konjac seed taro in Example 13 corresponding to Experimental Group 13.

[0092] (2) Experimental Group 1 and Experimental Group 2: The germination rate, germination time, growth rate and mass growth rate of Experimental Group 2 were all better than those of Experimental Group 1. This is because the reagent in Example 2 corresponding to Experimental Group 2 also includes α-amylase. The starch content of Amorphophallus konjac is high, and α-amylase can catalyze the hydrolysis of α-1,4-glycosidic bonds in starch molecules, breaking them down into smaller sugar molecules, such as maltose and glucose. These smaller sugar molecules are more easily absorbed and utilized by Amorphophallus konjac, providing energy and carbon source for the germination of Amorphophallus konjac.

[0093] (3) Experimental Groups 2 to 8: With the increase of enzyme activity, the germination rate, germination time, growth rate and mass growth rate showed a trend of first rising and then falling, and reached a peak in Experimental Group 6, which was 97.4% germination rate, 3.4 days germination time, 1.3 cm / day growth rate and 36.2% mass growth rate. This is because, under the condition of pH 6.0, the activity of α-amylase will increase with the increase of concentration between 25u / mL and 35u / mL, but after exceeding the threshold of 35u / mL, the excessive enzyme concentration will produce an inhibitory effect, which will reduce the germination of Amorphophallus taro.

[0094] (4) Experimental groups 2, 4, 6, 8 and experimental groups 9, 10, 11, 12: The germination rate, germination time, growth rate and mass growth rate of experimental groups 9, 10, 11, 12 under the same conditions were better than those of the corresponding experimental groups 2, 4, 6, 8. This is because 25 mg / L potassium nitrate, 40 mg / L magnesium sulfate and 35 mg / L superphosphate were added to the reagents in Example 9-12 corresponding to the experimental groups 9-12, providing additional mineral elements for the germination of the Amorphophallus konjac seed taro, including calcium and magnesium, which are involved in the regulation of α-amylase activity and the maintenance of cell structure.

[0095] Exemplary devices:

[0096] like Figure 1 As shown, a device for preparing a reagent for promoting rapid germination of konjak seed taro comprises a kettle 1, an auxin regulating pool 2, an enzyme preparation regulating pool 3, a mineral preparation regulating pool 4 and a plurality of liquid guiding mechanisms 5; a plurality of medium introduction ports 11 are arranged at the top of the kettle 1, and a liquid outlet valve 12 is arranged at the bottom; the kettle 1 is respectively connected with the auxin regulating pool 2, the enzyme preparation regulating pool 3 and the mineral preparation regulating pool 4 through the liquid guiding mechanism 5. By arranging the kettle 1, the auxin regulating pool 2, the enzyme preparation regulating pool 3, the mineral preparation regulating pool 4 and the liquid guiding mechanism 5, the mixing of auxin, enzyme preparation and mineral is achieved, and a reagent for promoting rapid germination of konjak seed taro is prepared.

[0097] like Figure 2 , Figure 3As shown, the liquid guiding mechanism 5 comprises an elastic hose 51, a gland 52 disposed on the elastic hose 51, a roller 53 disposed in the gland 52, two rollers 54 disposed oppositely on the roller 53, and a driver 55 for providing rotational power to the roller 53. The elastic hose 51 is fixed between the gland 52 and the roller 53 in an arc shape. Driven by the driver 55, the roller 53 rotates, and the roller 54 squeezes and releases the elastic hose 51 along the direction of the elastic hose 51. By controlling the number of rotations of the roller 53, the flow rate of the liquid in the elastic hose 51 is accurately controlled. The driver 55 can be a motor, a cylinder or other forms of driving devices that provide rotational power to the roller 53.

[0098] like Figure 1 As shown, the device further includes a pH adjustment mechanism 6; the pH adjustment mechanism 6 includes a pH monitor 61 and a pH adjustment tank 62; the detection end of the pH monitor 61 is inserted into the kettle body 1, and is used to monitor the pH value of the mixed solution in the kettle body 1; the pH adjustment tank 62 includes an acidic tank 621 and an alkaline tank 622, and both the acidic tank 621 and the alkaline tank 622 are connected to the kettle body 1 through a liquid guide mechanism 5. The pH monitor 61 monitors the pH value of the mixed solution in the kettle body 1, and controls the introduction of the solution in the acidic tank 621 or the solution in the alkaline tank 622 through the liquid guide mechanism 5 according to the monitoring result to adjust the pH value, ensure that the pH value of the mixed solution is appropriate, and improve the activity of the reagent.

[0099] like Figure 1 As shown, the device also includes an osmotic pressure regulating mechanism 7; the osmotic pressure regulating mechanism 7 includes an osmotic pressure monitoring meter 71 and an osmotic pressure regulating pool 72; the detection end of the osmotic pressure monitoring meter 71 is inserted into the kettle body 1, and is used to monitor the pH value of the mixed solution in the kettle body 1; the osmotic pressure regulating pool 72 includes a hypertonic solution pool 721 and a hypotonic solution pool 722, and the hypertonic solution pool 721 and the hypotonic solution pool 722 are both connected to the kettle body 1 through the liquid guiding mechanism 5. The osmotic pressure monitoring meter 71 monitors the osmotic pressure of the mixed solution in the kettle body 1, and controls the introduction of the solution in the hypertonic solution pool 721 or the solution in the hypotonic solution pool 722 through the liquid guiding mechanism 5 according to the monitoring result to adjust the osmotic pressure and ensure that the osmotic pressure of the mixed solution is suitable for the absorption of the konjac seed taro.

[0100] like Figure 1 As shown, a magnetic stirring generator 8 is also provided at the bottom of the kettle body 1; the stirring end of the magnetic stirring generator 8 extends into the interior of the kettle body 1. The magnetic stirring generator 8 acts on the stirring end in the kettle body 1 through magnetic force to achieve contactless stirring, uniformly mix the solution in the kettle body 1, and improve the uniformity and efficiency of reagent preparation.

[0101] The device also includes a control unit, which is connected to each driver 55 and the magnetic stirring generator 8 by signal, and controls the flow rate of the liquid through the liquid guide mechanism 5 and the operating state of the magnetic stirring generator 8. The control unit controls the driver 55 to drive the roller 53 to rotate according to the reagent components to be prepared, and accurately controls the content of each material introduced into the kettle body 1 by controlling the number of rotations of the roller 53 and the roller 54 set on the roller 53. At the same time, the control unit controls the magnetic stirring generator 8 to start or stop, and the stirring end of the magnetic stirring generator 8 starts or stops stirring.

[0102] like Figure 1 As shown, a jacket 13 is arranged around the kettle body 1; the jacket 13 is hollow inside, and a heat transfer medium inlet 131 and a heat transfer medium outlet 132 are arranged on both sides of the jacket 13. The temperature of the solution in the kettle body 1 is controlled by the inflow and outflow of the heat transfer medium, ensuring that the solution reacts at a suitable temperature.

[0103] like Figure 1 As shown, a sampling valve 14 is also provided at the bottom of the kettle body 1 for taking out a solution sample from the kettle body 1. By opening the sampling valve 14, the solution sample can be directly taken out from the kettle body 1, which is convenient for taking out and analyzing the solution sample.

[0104] like Figure 1 As shown, a window 15 is also provided on the top of the kettle body 1 for observing the internal situation of the kettle body 1. The state of the solution in the kettle body 1 can be directly observed through the window 15, and the preparation conditions can be adjusted in time.

[0105] When the device is in use, GA3, KT and NAA are added into the auxin regulating pool 2 at a mass ratio of 1:40:10, and water is introduced as a solvent to prepare an auxin solution; α-amylase solution is introduced into the enzyme preparation regulating pool 3; the prepared mineral solution is introduced into the mineral regulating pool 4; the prepared 5% citric acid solution is introduced into the acidic pool 621, and the prepared 5% sodium bicarbonate solution is introduced into the alkaline pool 622; the prepared 5% potassium chloride solution is introduced into the hypertonic solution pool 721, and water is introduced into the hypotonic solution pool 722.

[0106] According to the reagent formula, the auxin solution, α-amylase solution and mineral solution are introduced into the kettle body 1 through the liquid guide mechanism 5 in proportion, and the magnetic stirring generator 8 is turned on to mix the mixed solution evenly.

[0107] The pH monitor 61 continuously monitors the pH value of the mixed solution in the kettle 1, and controls the pH regulating pool 62 to add an acidic pool solution or an alkaline pool solution into the kettle 1 according to the pH value to adjust the pH value of the mixed solution; the osmotic pressure monitor 71 continuously monitors the osmotic pressure of the mixed solution in the kettle 1, and controls the osmotic pressure regulating pool 72 to add a hypertonic solution or a hypotonic solution into the kettle 1 according to the osmotic pressure to adjust the osmotic pressure of the mixed solution. The jacket 13 is provided with a heat transfer medium inlet 131 and a heat transfer medium outlet 132. The heat transfer medium is introduced into the heat transfer medium inlet 131, and the heat transfer medium is discharged from the heat transfer medium outlet 132 after circulating in the jacket 13, so as to control the mixed temperature of the solution in the kettle 1.

[0108] A solution sample is taken out from the kettle body 1 through the sampling valve 14 to analyze whether the sample meets the production requirements. If it meets the requirements, the liquid in the kettle body 1 is discharged into the collection container through the lower liquid outlet valve 12. If it does not meet the requirements, the ratio is adjusted.

[0109] Exemplary methods:

[0110] like Figure 4 As shown, a method for preparing a reagent for promoting rapid germination of konjac seed taro comprises the following steps:

[0111] S1, mixing GA3, KT, NAA and water to obtain an auxin solution;

[0112] S2, adding an enzyme preparation to the auxin solution to obtain an active reagent;

[0113] S3, adding mineral solution to the active agent;

[0114] S4. Adjust the pH value and osmotic pressure of the active reagent in S3 to obtain a reagent that promotes rapid germination of konjac seeds.

[0115] The concentrations of GA3, KT and NAA in the reagent for promoting rapid germination of konjac seeds are 1-2 mg / L, 40-60 mg / L and 10-20 mg / L respectively; the enzyme preparation is alpha-amylase with an activity of 25-40 u / mL; the mineral solution is a mixed solution of one or more of 15-25 mg / L ammonium nitrate, 20-25 mg / L potassium nitrate, 30-45 mg / L magnesium sulfate and 30-40 mg / L superphosphate; the pH value is 5.5-6.8, and the osmotic pressure is 50-500 mOsm / kg.

[0116] The above is based on the ideal embodiment of the present invention. Through the above description, relevant personnel can make various changes and modifications without departing from the technical concept of the present invention. The technical scope of the present invention is not limited to the content in the specification, and the technical scope must be determined according to the scope of the claims.

Claims

1. A reagent for promoting rapid germination of konjac taro, the formula of which is: 1-2 mg / L GA3, 40-60 mg / L KT, 10-20 mg / L NAA and enzyme preparation, and the solvent is deionized water.

2. A kind of reagent for promoting rapid germination of konjac taro according to claim 1, characterized in that, The enzyme preparation is one of α-amylase, β-amylase, glucoamylase and amyloglucosidase.

3. A kind of reagent promoting rapid germination of konjac taro according to claim 2, characterized in that, The activity of the enzyme preparation is 25-40u / mL.

4. A kind of reagent for promoting rapid germination of konjac taro according to claim 3, characterized in that, The pH value of the reagent is 5.5-6.8, and the osmotic pressure is 50-500 mOsm / kg.

5. A kind of reagent for promoting rapid germination of konjac taro according to claim 1, characterized in that, The reagents also include one or more of: 15-25 mg / L ammonium nitrate, 20-25 mg / L potassium nitrate, 30-45 mg / L magnesium sulfate and 30-40 mg / L superphosphate.

6. A device for preparing a reagent for promoting rapid germination of konjac taro, for preparing the reagent according to any one of claims 1 to 5, characterized in that: The invention comprises a kettle body (1), a growth hormone regulating pool (2), an enzyme preparation regulating pool (3), a mineral preparation regulating pool (4) and a plurality of liquid guiding mechanisms (5); the top of the kettle body (1) is provided with a plurality of medium inlet ports (11), the bottom is provided with a liquid outlet valve (12), a jacket (13) is provided outside the kettle body (1), a sampling valve (14) is provided at the bottom of the kettle body (1), and a window (15) is provided at the top of the kettle body (1); the kettle body (1) is respectively connected to the growth hormone regulating pool (2), the enzyme preparation regulating pool (3) and the mineral preparation regulating pool (4) through the liquid guiding mechanisms (5); the liquid guiding mechanisms (5) comprise an elastic hose (51), a pressure cover (52) provided on the elastic hose (51), a roller (53) provided in the pressure cover (52), a roller (54) provided on the roller (53) and a driver (55) for providing rotational power to the roller (53).

7. A kind of reagent preparation device for promoting rapid germination of konjac seed taro according to claim 6, characterized in that, The device further comprises a pH adjustment mechanism (6); the pH adjustment mechanism (6) comprises a pH monitor (61) and a pH adjustment pool (62); the detection end of the pH monitor (61) is inserted into the kettle body (1) for monitoring the pH value of the mixed solution in the kettle body (1); the pH adjustment pool (62) comprises an acid pool (621) and an alkaline pool (622), and both the acid pool (621) and the alkaline pool (622) are connected to the kettle body (1) via the liquid guide mechanism (5).

8. A kind of reagent preparation device for promoting rapid germination of konjac seed taro according to claim 7, characterized in that, The device further comprises an osmotic pressure regulating mechanism (7); the osmotic pressure regulating mechanism (7) comprises an osmotic pressure monitor (71) and an osmotic pressure regulating pool (72); the detection end of the osmotic pressure monitor (71) is inserted into the kettle body (1) for monitoring the pH value of the mixed solution in the kettle body (1); the osmotic pressure regulating pool (72) comprises a hypertonic solution pool (721) and a hypotonic solution pool (722), and the hypertonic solution pool (721) and the hypotonic solution pool (722) are both connected to the kettle body (1) via the liquid guiding mechanism (5).

9. A kind of reagent preparation device for promoting rapid germination of konjac seed taro according to claim 8, characterized in that, A magnetic stirring generator (8) is also provided at the bottom of the kettle body (1); the stirring end of the magnetic stirring generator (8) extends into the interior of the kettle body (1).

10. A method for preparing a reagent for promoting rapid germination of konjac taro, for preparing the rapid germination reagent according to any one of claims 1 to 5, characterized in that: The following steps are involved: S1, mixing GA3, KT and NAA with deionized water to obtain an auxin solution; S2, adding an enzyme preparation to the auxin solution to obtain an active reagent; S3, adding mineral solution to the active agent; S4, adjusting the pH value and osmotic pressure of the active reagent in S3 to obtain a reagent for promoting rapid germination of konjac seed taro; The concentrations of GA3, KT and NAA in the reagent for promoting rapid germination of konjac seeds are 1-2 mg / L, 40-60 mg / L and 10-20 mg / L respectively; the enzyme preparation is alpha-amylase with an activity of 25-40 u / mL; the mineral solution is a mixed solution of one or more of 15-25 mg / L ammonium nitrate, 20-25 mg / L potassium nitrate, 30-45 mg / L magnesium sulfate and 30-40 mg / L superphosphate; the pH value is 5.5-6.8, and the osmotic pressure is 50-500 mOsm / kg.

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