Analysis device and analysis method for lignocellulose degrading bacteria

Abstract

The invention is applicable to the related technical field of microorganisms, and provides a lignocellulose degrading bacterium analysis device and method.The device comprises a supporting base, a mounting frame is fixedly connected to the top of the supporting base, a culture mechanism is fixedly connected to the inner side of the mounting frame, and a first motor is fixedly connected to the outer side of the mounting frame; the device comprises a mounting frame, a supporting base is arranged on the top of the mounting frame, a reinforcing rod is fixedly connected to the inner side of the supporting base, a transverse moving mechanism is arranged at the top of the mounting frame, and a rotary observation mechanism is arranged at the bottom of the transverse moving mechanism. According to the device, the inner bacterial colony and a solution can be fully mixed conveniently, meanwhile, the bacterial colony in the culture test tube can be ensured to obtain the same culture condition through the rotation design of the culture test tube, the subsequent accurate analysis of lignocellulose degrading bacteria is facilitated, and the analysis accuracy is ensured.

Description

Technical Field

[0001] The invention belongs to the technical field related to microorganisms, and in particular relates to an analysis device and an analysis method for lignocellulose degrading bacteria. Background Art

[0002] Lignocellulose-degrading bacteria are a type of microorganism that can decompose lignocellulose. Lignocellulose-degrading bacteria can secrete a variety of extracellular enzymes, such as cellulases, hemicellulases and ligninases. These enzymes can decompose lignocellulose into small molecular sugars and other organic compounds for absorption and utilization by microorganisms. Some degrading bacteria participate in the degradation of lignin by secreting oxidoreductases, such as laccase and peroxidase. These enzymes can catalyze the oxidation reaction of phenolic hydroxyl groups in lignin molecules, making their structure loose and easier to be decomposed by other enzymes.

[0003] Lignocellulose-degrading bacteria can be used in biopulping, bioenergy production and environmental protection. By utilizing the selective degradation of lignin by white rot fungi and other fungi, wood or other fiber raw materials can be pretreated before pulping, which can reduce pulping energy consumption, improve paper quality, and reduce the use of chemical agents. Lignocellulose-degrading bacteria can convert lignocellulose into fermentable sugars, and then produce bioenergy such as bioethanol and biodiesel through fermentation, providing a new way to solve the energy crisis. It can be used to treat organic waste containing lignocellulose, such as crop straw, wood processing waste, etc., and convert them into organic fertilizers or soil conditioners to reduce the pollution of waste to the environment.

[0004] The lignocellulose degrading bacteria analysis device is used to perform colony morphology analysis and enzyme activity detection, and further study the optimal temperature, pH, substrate specificity and other characteristics of the enzymes secreted by the degrading bacteria. By using an ELISA instrument to detect enzyme activity under different temperature and pH conditions, the enzyme activity condition curve is drawn and the optimal action conditions of the enzyme are determined. This helps to understand the degradation efficiency of degrading bacteria in different environments and also provides a basis for the use and optimization of enzymes in industrial applications.

[0005] At present, most of the culture dishes used in analytical devices use separate incubators. It is difficult to ensure the same culture environment for the culture dishes in the incubator, which may cause deviations in the analysis of lignocellulose-degrading bacteria and affect the analysis results. Summary of the invention

[0006] The purpose of the present invention is to provide an analysis device and an analysis method for lignocellulose degrading bacteria, aiming to solve the problem that the culture environment is affected due to placement problems in a conventional incubator, thereby causing analysis deviations.

[0007] The purpose of the present invention can be achieved through the following technical solutions: A device for analyzing lignocellulose-degrading bacteria comprises a support base, a mounting frame fixedly connected to the top of the support base, a cultivation mechanism fixedly connected to the inner side of the mounting frame, a first motor fixedly connected to the outer side of the mounting frame, a reinforcement rod fixedly connected to the inner side of the support base, a transverse movement mechanism provided on the top of the mounting frame, a rotating observation mechanism provided on the bottom of the transverse movement mechanism, and a transverse movement drive unit provided on one side of the other end of the mounting frame.

[0008] The culture mechanism includes a mounting frame, the outer side of the mounting frame is fixedly connected to a fixed rail, the inner side of the fixed rail is provided with an opening and closing door, the inner side of the mounting frame is rotatably connected to a rotating mounting seat, a culture test tube is installed on the inner side of the rotating mounting seat, a connecting rod is fixedly connected to the middle position of the inner side of the culture test tube, two lighting lamps are fixedly connected to the upper side of the rotating mounting seat, and the two lighting lamps are respectively located at the upper and lower ends of the interior of the mounting frame, the output shaft of the first motor passes through the mounting frame and is fixedly connected to the rotating mounting seat, and the rotating mounting seat is driven to rotate by the first motor, so that the culture test tube installed in the rotating mounting seat is evenly rotated, which is convenient for fully mixing the internal bacterial colony and solution. At the same time, the rotation design of the culture test tube can ensure that the bacterial colony in the culture test tube can obtain the same culture conditions, which is convenient for the subsequent accurate analysis of lignocellulose degrading bacteria and ensures the accuracy of the analysis.

[0009] According to the above technical solution, the rotating mounting seat includes a mounting block, the mounting block is rotatably connected to the inner wall of the mounting frame, a clamping groove is opened inside the mounting block, a fixed block is fixedly installed on one side of the clamping groove, the internal thread of the fixed block is rotatably connected to a rotating screw, and the end of the rotating screw away from the clamping groove is fixedly connected to the rotating block. The rotating screw is driven to rotate in the fixed block by the rotating block, and the rotating screw can be moved to close the clamping groove, thereby ensuring that the culture test tube is installed inside the rotating mounting seat, ensuring that it is firmly fixed, and facilitating the cultivation of lignocellulose degrading bacteria.

[0010] According to the above technical solution, the culture tube includes a tube body, one end of which is provided with a tube head, and the other end of which is provided with a splicing block, through which two tube bodies can be inserted simultaneously to increase the analysis volume.

[0011] According to the above technical solution, the tube head includes a tube cover, one end of which is fixedly connected to a clamping block, the size of the clamping block is consistent with the size of the clamping groove, the tube cover is clamped and connected to one end of the tube body, and the tube body is closed by the tube head and is used for clamping installation on the rotating mounting seat.

[0012] According to the above technical solution, the number of the snap-in slots is provided in three groups, which, together with the two tube bodies that can be installed at the same time, can be used to simultaneously install six culture tubes, thereby ensuring that multiple groups of analysis reference groups can be set up in the same link, and multiple comparative analysis can be performed, thereby improving the analysis accuracy.

[0013] According to the above technical solution, the transverse movement mechanism includes a transmission screw, which is rotatably connected to the inner side of the mounting frame. Limiting plates are installed on both sides of the transmission screw. The external thread of the limiting plate is rotatably connected to a moving block. The moving block is driven to move by the rotation of the transmission screw, thereby driving the movement of the rotating observation mechanism, so as to adjust the observation position of the rotating observation mechanism.

[0014] According to the above technical solution, the rotating observation mechanism includes a fixed outer ring, which is fixedly connected to the bottom of the moving block, and the internal rotation of the fixed outer ring is connected to a rotating inner ring. A fixed plate is provided at the bottom of the rotating inner ring, and a monitoring camera is installed at the bottom of the fixed plate. A mounting plate is fixedly connected to one side of the fixed outer ring, and a second motor is fixedly connected to the top of the mounting plate. A synchronous rotating block is provided at the bottom of the second motor, and the synchronous rotating block and the inner side of the rotating inner ring are clamped and connected. The synchronous rotating block is driven to rotate by the second motor, so that the synchronous rotating block can drive the rotating inner ring to rotate in the fixed outer ring, thereby driving the monitoring camera to move, so as to facilitate the adjustment of the position of the monitoring camera, and is used to observe culture tubes at different positions and check the culture conditions.

[0015] According to the above technical solution, the rotating inner ring includes ring teeth, and the ring teeth are rotatably installed inside the fixed outer ring. One end of the ring teeth is fixedly connected to a connecting block, and the bottom of the connecting block is fixedly connected to the top of the fixed plate, ensuring that the rotation of the rotating inner ring can drive the monitoring camera to move, and cooperate with the transverse movement mechanism to adjust the position of the monitoring camera, so as to facilitate multi-angle observation of the cultivation mechanism and facilitate recording, which is beneficial to the analysis of lignocellulose degrading bacteria.

[0016] According to the above technical solution, the transverse driving unit includes a shell, which is fixedly connected to one side of the mounting frame, and the inside of the shell is rotatably connected to two transmission rods, and the outer sides of the two transmission rods are sleeved with transmission belts. The outer side of the shell is fixedly connected to a third motor, and the output shaft of the third motor passes through the shell and is fixedly connected to the transmission rod located at the lower part of the shell. The two transmission rods are respectively fixedly connected to two transmission screws, and the moving block is driven to move by the third motor.

[0017] A method for analyzing lignocellulose-degrading bacteria, comprising the following steps: Step 1: inoculate lignocellulose-degrading bacteria into six groups of culture tubes and culture them in the installation frame at the same temperature and humidity; Step 2: After the culture is completed, the size, shape, color, texture and edge characteristics of the colonies in the culture tube are recorded and analyzed; Step 3: Make a temporary slide specimen. For the liquid cultured lignocellulose-degrading bacteria, take a drop of bacterial liquid from each of the six groups of culture tubes and drop it on the slide, then cover it lightly with a cover glass for staining and fixation. Step 4: Observe the shape, size, and arrangement of cells under an optical microscope and make a record; Step 5: Use a cellulose substrate containing a fluorescent group. When cellulase acts on the substrate, a fluorescent product will be released. After the enzyme solution and the substrate react under appropriate conditions, use a fluorescence spectrophotometer to detect the fluorescence intensity at a wavelength of 400-500nm, and calculate the enzyme activity based on the standard curve.

[0018] The beneficial effects of the present invention are as follows: the first motor drives the rotating mounting seat to rotate, so that the culture tube installed in the rotating mounting seat rotates evenly, which is convenient for fully mixing the internal bacterial colonies and solution. At the same time, the rotating design of the culture tube can ensure that the bacterial colonies in the culture tube can obtain the same culture conditions, which is convenient for the subsequent accurate analysis of lignocellulose degrading bacteria and ensures the accuracy of the analysis.

[0019] Of course, any product implementing the present invention does not necessarily need to achieve all of the advantages described above at the same time. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings required for describing the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other accompanying drawings can be obtained based on these accompanying drawings without paying creative work.

[0021] Figure 1 It is a schematic diagram of the overall structure of an analysis device for lignocellulose-degrading bacteria provided by the present invention; Figure 2 This is a schematic diagram of the structure of a culture mechanism of an analysis device for lignocellulose-degrading bacteria provided by the present invention; Figure 3 This is a schematic diagram of the structure of a rotating mounting base of an analysis device for lignocellulose-degrading bacteria provided by the present invention; Figure 4 This is a schematic diagram of the culture tube structure of a device for analyzing lignocellulose-degrading bacteria provided by the present invention; Figure 5 This is a schematic diagram of the tube head structure of a device for analyzing lignocellulose-degrading bacteria provided by the present invention; Figure 6This is a schematic diagram of the structure of a transverse movement mechanism of an analysis device for lignocellulose-degrading bacteria provided by the present invention; Figure 7 This is a schematic diagram of the structure of a rotating observation mechanism of an analysis device for lignocellulose-degrading bacteria provided by the present invention; Figure 8 This is a schematic diagram of the rotating inner ring structure of an analysis device for lignocellulose-degrading bacteria provided by the present invention; Fig. 9 It is a schematic diagram of the structure of a lateral drive unit of an analysis device for lignocellulose-degrading bacteria provided by the present invention.

[0022] Notes on reference numerals: 1. Support base; 2. Mounting frame; 3. Cultivation mechanism; 4. First motor; 5. Reinforcement rod; 6. Transverse movement mechanism; 7. Rotation observation mechanism; 8. Transverse movement drive unit; 301. Mounting frame; 302. Fixed rail; 303. Opening and closing door; 304. Rotation mounting seat; 305. Cultivation test tube; 306. Connecting rod; 307. Illumination lamp; 310. Mounting block; 311. Clamping groove; 312. Fixed block; 313. Rotation screw; 314. Rotation block; 320 , tube body; 321, tube head; 322, splicing block; 330, tube cover; 331, clamping block; 601, limit plate; 602, transmission screw; 603, moving block; 701, fixed outer ring; 702, rotating inner ring; 703, mounting plate; 704, second motor; 705, synchronous rotating block; 706, fixed plate; 707, monitoring camera; 710, ring gear; 711, connecting block; 801, outer shell; 802, transmission rod; 803, transmission belt; 804, third motor. DETAILED DESCRIPTION

[0023] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0024] See also Figure 1-Figure 9 As shown, the present invention is an analysis device for lignocellulose degrading bacteria, comprising a support base 1, a mounting frame 2 is fixedly connected to the top of the support base 1, a cultivation mechanism 3 is fixedly connected to the inner side of the mounting frame 2, a first motor 4 is fixedly connected to the outer side of the mounting frame 2, a reinforcement rod 5 is fixedly connected to the inner side of the support base 1, a transverse mechanism 6 is arranged on the top of the mounting frame 2, a rotating observation mechanism 7 is arranged on the bottom of the transverse mechanism 6, and a transverse driving unit 8 is arranged on one side of the other end of the mounting frame 2.

[0025] The culture mechanism 3 includes a mounting frame 301, the outer side of the mounting frame 301 is fixedly connected to a fixed rail 302, the inner side of the fixed rail 302 is provided with an opening and closing door 303, the inner side of the mounting frame 301 is rotatably connected to a rotating mounting seat 304, the inner side of the rotating mounting seat 304 is provided with a culture tube 305, the middle position of the inner side of the culture tube 305 is fixedly connected to a connecting rod 306, the upper side of the rotating mounting seat 304 is fixedly connected to two lighting lamps 307, the two lighting lamps 307 are respectively located at the upper and lower ends inside the mounting frame 301, and the output shaft of the first motor 4 passes through the mounting frame 301 and is fixedly connected to the rotating mounting seat 304.

[0026] The first motor 4 drives the rotating mounting seat 304 to rotate, so that the culture tube 305 installed in the rotating mounting seat 304 rotates evenly, which is convenient for fully mixing the internal bacterial colonies and solution. At the same time, the rotating design of the culture tube 305 can ensure that the bacterial colonies in the culture tube 305 can obtain the same culture conditions, which is convenient for the subsequent accurate analysis of the lignocellulose degrading bacteria and ensures the accuracy of the analysis.

[0027] Combination Figure 3 , Figure 4 and Figure 5 The rotatable mounting seat 304 includes a mounting block 310, which is rotatably connected to the inner wall of the mounting frame 301. A snap-in groove 311 is provided inside the mounting block 310, and a fixed block 312 is fixedly installed on one side of the snap-in groove 311. The internal thread of the fixed block 312 is rotatably connected to a rotating screw 313, and the end of the rotating screw 313 away from the snap-in groove 311 is fixedly connected to a rotating block 314. The rotating screw 313 is driven to rotate in the fixed block 312 by the rotating block 314, and the rotating screw 313 can be moved to close the snap-in groove 311, so as to ensure that the culture tube 305 is installed inside the rotatable mounting seat 304, and the fixation is firm, which is convenient for the cultivation of lignocellulose degrading bacteria.

[0028] The culture test tube 305 includes a tube body 320, one end of which is provided with a tube head 321, and the other end of the tube body 320 is provided with a splicing block 322. Two tube bodies 320 can be inserted at the same time through the splicing block 322 to increase the analysis amount. The tube head 321 includes a tube cover 330, one end of the tube cover 330 is fixedly connected with a clamping block 331, the size of the clamping block 331 is consistent with the size of the clamping groove 311, the tube cover 330 is clamped and connected to one end of the tube body 320, the tube body 320 is closed by the tube head 321 and is used for clamping and installation on the rotating mounting seat 304, the number of the clamping grooves 311 is provided with three groups, and with the two tube bodies 320 that can be installed at the same time, it can be used to install six culture test tubes 305 at the same time, ensuring that multiple groups of analysis reference groups can be set in the same link, multiple comparative object analysis can be performed, and the analysis accuracy is improved.

[0029] Combination Figure 6-Figure 9 The transverse movement mechanism 6 includes a transmission screw 602, which is rotatably connected to the inner side of the mounting frame 2. Limiting plates 601 are installed on both sides of the transmission screw 602. The external thread of the limiting plate 601 is rotatably connected to a moving block 603. The moving block 603 is driven to move by the rotation of the transmission screw 602, thereby driving the movement of the rotating observation mechanism 7, so as to adjust the observation position of the rotating observation mechanism 7.

[0030] The rotating observation mechanism 7 includes a fixed outer ring 701, which is fixedly connected to the bottom of the moving block 603. The fixed outer ring 701 is internally rotatably connected to a rotating inner ring 702. A fixed plate 706 is provided at the bottom of the rotating inner ring 702. A monitoring camera 707 is installed at the bottom of the fixed plate 706. A mounting plate 703 is fixedly connected to one side of the fixed outer ring 701. A second motor 704 is fixedly connected to the top of the mounting plate 703. A synchronous rotating block 705 is provided at the bottom of the second motor 704. The synchronous rotating block 705 is connected to the inner side of the rotating inner ring 702 by snap-fitting. The synchronous rotating block 705 is driven to rotate by the second motor 704, so that the synchronous rotating block 705 can drive the rotating inner ring 702 to rotate in the fixed outer ring 701, thereby driving the monitoring camera 707 to move, so as to facilitate the adjustment of the position of the monitoring camera 707, so as to observe the culture tubes 305 at different positions and check the culture conditions.

[0031] The rotating inner ring 702 includes a ring tooth 710, and the ring tooth 710 is rotatably installed inside the fixed outer ring 701. One end of the ring tooth 710 is fixedly connected to a connecting block 711, and the bottom of the connecting block 711 is fixedly connected to the top of the fixed plate 706, ensuring that the rotation of the rotating inner ring 702 can drive the monitoring camera 707 to move, and cooperate with the transverse movement mechanism 6 to adjust the position of the monitoring camera 707, so as to facilitate multi-angle observation of the cultivation mechanism 3 and facilitate recording, which is beneficial to the analysis of lignocellulose degrading bacteria.

[0032] The transverse driving unit 8 includes a shell 801, which is fixedly connected to one side of the mounting frame 2. Two transmission rods 802 are rotatably connected inside the shell 801, and the outer sides of the two transmission rods 802 are sleeved with a transmission belt 803. A third motor 804 is fixedly connected to the outer side of the shell 801. The output shaft of the third motor 804 passes through the shell 801 and is fixedly connected to the transmission rod 802 located at the lower part of the shell 801. The two transmission rods 802 are respectively fixedly connected to the two transmission screw rods 602, and the moving block 603 is driven to move by the third motor 804.

[0033] In summary, different proportions of lignocellulose degrading bacteria and solutions are added into the tube body 320 respectively to obtain six groups of culture tubes 305 with different proportions. Then, the proportioned test tubes are inserted into the interior of the rotating mounting seat 304 through the tube head 321. After the culture tubes 305 are fixed using the rotating screw 313, the opening and closing door 303 is closed, the illumination lamp 307 is turned on, and then the first motor 4 is started to drive the rotating mounting seat 304 to rotate, and the culture tubes 305 are rotated and mixed to ensure that the bacteria and the solution can be fully mixed. After the mixing is completed, the rotation speed of the culture tubes 305 is lowered. When the monitoring camera 707 needs to be used for recording, the rotation of the culture tubes 305 is stopped. The third motor 804 cooperates with the second motor 704 to adjust the observation angle of the monitoring camera 707 to facilitate the recording of lignocellulose degrading bacteria. Then, the cultured bacteria are taken out and observed using an optical microscope, and the enzyme activity is measured through physiological and biochemical analysis.

[0034] A method for analyzing lignocellulose degrading bacteria, wherein the lignocellulose degrading bacteria are inoculated in six groups of culture test tubes 305, cultured in a mounting frame 301 at the same temperature and humidity, and after the culture is completed, the size, shape, color, texture and edge characteristics of the colonies in the culture test tubes 305 are recorded and analyzed, and a temporary glass slide specimen is prepared. For the lignocellulose degrading bacteria cultured in liquid, a drop of bacterial liquid is taken from each of the six groups of culture test tubes 305 and dropped on a glass slide, which is gently covered with a cover glass for staining and fixing. Under an optical microscope, the shape, size and arrangement of the cells are observed and recorded, a cellulose substrate containing a fluorescent group is used, and when cellulase acts on the substrate, a fluorescent product is released, and after the enzyme solution and the substrate react under appropriate conditions, the fluorescence intensity is detected at a wavelength of 400-500nm using a fluorescence spectrophotometer, and the enzyme activity is calculated according to a standard curve.

[0035] The above contents are merely examples and explanations of the concept of the present invention. The technicians in this technical field may make various modifications or additions to the specific embodiments described or replace them in a similar manner. As long as they do not deviate from the concept of the invention or exceed the scope defined by the claims, they should all fall within the protection scope of the present invention.

Claims

1. A device for analyzing lignocellulose-degrading bacteria, comprising a support base (1), characterized in that: The top of the support base (1) is fixedly connected to a mounting frame (2), the inner side of the mounting frame (2) is fixedly connected to a culture mechanism (3), the outer side of the mounting frame (2) is fixedly connected to a first motor (4), the inner side of the support base (1) is fixedly connected to a reinforcement rod (5), the top of the mounting frame (2) is provided with a transverse movement mechanism (6), the bottom of the transverse movement mechanism (6) is provided with a rotation observation mechanism (7), and one side of the other end of the mounting frame (2) is provided with a transverse movement drive unit (8); The culture mechanism (3) comprises a mounting frame (301), the outer side of the mounting frame (301) is fixedly connected to a fixed rail (302), the inner side of the fixed rail (302) is provided with an opening and closing door (303), the inner side of the mounting frame (301) is rotatably connected to a rotating mounting seat (304), a culture test tube (305) is mounted on the inner side of the rotating mounting seat (304), a connecting rod (306) is fixedly connected to the middle position of the inner side of the culture test tube (305), two lighting lamps (307) are fixedly connected above the rotating mounting seat (304), and the two lighting lamps (307) are respectively located at the upper and lower ends of the inner side of the mounting frame (301), and the output shaft of the first motor (4) passes through the mounting frame (301) and is fixedly connected to the rotating mounting seat (304).

2. The analysis device for lignocellulose-degrading bacteria according to claim 1, characterized in that: The rotatable mounting seat (304) comprises a mounting block (310), the mounting block (310) being rotatably connected to the inner wall of the mounting frame (301), a clamping groove (311) being provided inside the mounting block (310), a fixing block (312) being fixedly mounted on one side of the clamping groove (311), an internal thread of the fixing block (312) being rotatably connected to a rotating screw rod (313), and an end of the rotating screw rod (313) away from the clamping groove (311) being fixedly connected to a rotating block (314).

3. The analysis device for lignocellulose-degrading bacteria according to claim 1, characterized in that: The culture test tube (305) comprises a tube body (320), one end of the tube body (320) is provided with a tube head (321), and the other end of the tube body (320) is provided with a splicing block (322).

4. The analysis device for lignocellulose-degrading bacteria according to claim 3, characterized in that: The tube head (321) comprises a tube cover (330), one end of the tube cover (330) is fixedly connected to a clamping block (331), the size of the clamping block (331) is consistent with the size of the clamping groove (311), and the tube cover (330) is clamped and connected to one end of the tube body (320).

5. The analysis device for lignocellulose-degrading bacteria according to claim 2, characterized in that: The number of the clamping grooves (311) is three groups, and together with the two tube bodies (320) that can be installed simultaneously, six culture test tubes (305) can be installed simultaneously.

6. The analysis device for lignocellulose-degrading bacteria according to claim 1, characterized in that: The transverse movement mechanism (6) comprises a transmission screw (602), wherein the transmission screw (602) is rotatably connected to the inner side of the mounting frame (2), and limit plates (601) are installed on both sides of the transmission screw (602), and the outer threads of the limit plates (601) are rotatably connected to the moving blocks (603).

7. The analysis device for lignocellulose-degrading bacteria according to claim 1, characterized in that: The rotating observation mechanism (7) comprises a fixed outer ring (701), wherein the fixed outer ring (701) is fixedly connected to the bottom of the moving block (603), the interior of the fixed outer ring (701) is rotatably connected to a rotating inner ring (702), a fixed plate (706) is provided at the bottom of the rotating inner ring (702), a monitoring camera (707) is installed at the bottom of the fixed plate (706), one side of the fixed outer ring (701) is fixedly connected to a mounting plate (703), the top of the mounting plate (703) is fixedly connected to a second motor (704), a synchronous rotating block (705) is provided at the bottom of the second motor (704), and the synchronous rotating block (705) is snap-connected to the inner side of the rotating inner ring (702).

8. The analysis device for lignocellulose-degrading bacteria according to claim 7, characterized in that: The rotating inner ring (702) comprises a ring tooth (710), and the ring tooth (710) is rotatably mounted inside the fixed outer ring (701). One end of the ring tooth (710) is fixedly connected to a connecting block (711), and the bottom of the connecting block (711) is fixedly connected to the top of the fixed plate (706).

9. The analytical device for lignocellulose-degrading bacteria according to claim 1, characterized in that: The transverse drive unit (8) comprises a housing (801), wherein the housing (801) is fixedly connected to one side of the mounting frame (2), wherein two transmission rods (802) are rotatably connected inside the housing (801), and transmission belts (803) are sleeved on the outer sides of the two transmission rods (802), and a third motor (804) is fixedly connected to the outer side of the housing (801), and an output shaft of the third motor (804) passes through the housing (801) and is fixedly connected to the transmission rod (802) located at the lower part of the housing (801), and the two transmission rods (802) are respectively fixedly connected to two transmission screw rods (602).

10. A method for analyzing lignocellulose-degrading bacteria, characterized in that: An analysis device comprising the lignocellulose degrading bacteria according to any one of claims 1 to 9, comprising the steps of: Step 1: inoculating lignocellulose-degrading bacteria into six groups of culture tubes (305), and culturing them in the installation frame (301) at the same temperature and humidity; Step 2: After the culture is completed, the size, shape, color, texture and edge characteristics of the colonies in the culture tube (305) are recorded and analyzed; Step 3: Prepare a temporary slide specimen. For the liquid cultured lignocellulose-degrading bacteria, take a drop of bacterial liquid from each of the six groups of culture tubes (305) and drop it on a glass slide, then cover it lightly with a cover glass for staining and fixing. Step 4: Observe the shape, size, and arrangement of cells under an optical microscope and make a record; Step 5: Use a cellulose substrate containing a fluorescent group. When cellulase acts on the substrate, a fluorescent product will be released. After the enzyme solution and the substrate react under appropriate conditions, use a fluorescence spectrophotometer to detect the fluorescence intensity at a wavelength of 400-500nm, and calculate the enzyme activity based on the standard curve.

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