Separation device for human stem cell culture

Abstract

The invention relates to the technical field of stem cell culture and separation, in particular to a separation device for human stem cell culture, which comprises a base, a test tube clamping mechanism, an extraction and separation mechanism, a sealing mechanism, a liquid scraping mechanism and a disinfection mechanism, and the outer wall of the fifth mounting plate is movably connected with a first bearing frame. The extraction and separation mechanism is matched with the disinfection mechanism to clean and disinfect the outer walls of the sides, in contact with stem cells, of the closed discs during extraction and separation, bacteria are prevented from being brought into culture test tubes, meanwhile, one closed disc corresponds to one culture test tube, and confusion of experimental materials is avoided; the opening and closing of the closing mechanism can be controlled through the movement of the extraction and separation mechanism, and meanwhile, the closing mechanism is matched with the liquid scraping mechanism, so that when the extraction test tube moves upwards along with the telescopic rod, a first arc-shaped plate can promote residual liquid on the outer wall of the extraction test tube to quickly fall into a receiving groove, and time for waiting is not needed.

Description

Technical Field

[0001] The present invention relates to the technical field of stem cell culture and separation, and in particular to a separation device for human stem cell culture. Background Art

[0002] Stem cells are a type of multipotent cells with the ability to self-replicate. Under certain conditions, they can differentiate into multiple functional cells. According to the developmental stage of stem cells, they are divided into embryonic stem cells and adult stem cells. According to the developmental potential of stem cells, they are divided into three categories: totipotent stem cells, pluripotent stem cells and unipotent stem cells. Stem cells are a type of undifferentiated, immature cell that has the potential to regenerate various tissues, organs and the human body. The medical community calls them universal cells.

[0003] The invention patent with announcement number CN117903921B discloses an immune stem cell extraction and separation device and a method of using the same, including a base and multiple test tubes. A fixed plate is provided on the top of the base, a positioning plate is provided on the top of the fixed plate through a support rod, a lifting seat is provided above the positioning plate, and multiple extraction straws are provided in the lifting seat. A first support plate and a second support plate are respectively fixedly connected to both sides of the top of the base by bolts.

[0004] However, the above invention patent still has some shortcomings in actual use: 1. The magnetic adsorption of the iron block by the first magnet can make the closed disk rotate, so that the through holes and the test tubes in the fixed disk and the positioning disk are arranged alternately, so that when the stem cells are centrifuged, the stem cells in the test tubes can be prevented from being thrown out of the test tubes. However, this method makes the liquid inside the test tube stick to the closed disk. The subsequent rotation of the closed disk will cause the liquid in the test tube to move with the closed disk. The closed disk is very likely to bring bacteria into the test tube, and it is also very likely to confuse the experimental materials. 2. When the extraction of the extraction pipette is completed, the extraction pipette is outside Part of the solution attached to the wall drips into the collection tank along the outer wall of the test tube. If the liquid remaining on the outer wall of the extraction pipette is only caused to drip by gravity, if the waiting time is short, the liquid on the outer wall of the extraction pipette is not removed thoroughly enough. If the waiting time is too long, the liquid collected by the extraction pipette is very likely to deteriorate. 3. It is very inconvenient to replace the extraction pipette in the prior art. If you want to extract immune stem cell samples at different levels in a cell culture test tube, you need to replace the extraction pipettes one by one, which is very inefficient. Therefore, a separation device for human stem cell culture is proposed to solve the above-mentioned problems. Summary of the invention

[0005] The purpose of the present invention is to solve the problems in the background technology and to propose a separation device for culturing human stem cells.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions:

[0007] A separation device for culturing human stem cells, comprising a base, a top outer wall of the base being fixedly connected to a telescopic rod, a top outer wall of the telescopic rod being fixedly connected to a fifth mounting plate, an outer wall of the fifth mounting plate being movably connected to a first bearing frame, an inner wall of the base being fixedly connected to a centrifugal motor, an output shaft of the centrifugal motor being fixedly connected to a third mounting plate, a top outer wall of the third mounting plate being fixedly connected to two evenly distributed fixing columns, a top outer wall of the two fixing columns being fixedly connected to a second mounting plate, a top outer wall of the second mounting plate being fixedly connected to the first mounting frame, and further comprising:

[0008] A test tube clamping mechanism, used for clamping the culture test tube, the test tube clamping mechanism is mounted on the second mounting plate;

[0009] An extraction and separation mechanism, used for squeezing the air bag of the extraction straw, wherein the extraction and separation mechanism is mounted on the fifth mounting plate;

[0010] A closing mechanism, used to cooperate with the movement of the extraction and separation mechanism to close the culture tube, and the closing mechanism is installed on the first mounting frame;

[0011] A liquid scraping mechanism, used to cooperate with the extraction and separation mechanism so that the liquid on the outer wall of the extraction straw is discharged faster, and the liquid scraping mechanism is installed on the first mounting frame;

[0012] and a disinfection mechanism, which is used to disinfect the extraction and separation mechanism when the extraction and separation mechanism extracts stem cells, and the disinfection mechanism is installed on the fifth installation plate.

[0013] Preferably, one of the fixed columns has a knob assembly threadedly connected to its inner wall, a second shaft rod is slidably connected to the inner wall of the knob assembly, one end of the second shaft rod is fixedly connected to the first shaft rod, a raised column is fixedly connected to the outer wall of the first shaft rod, a limiting column is slidably connected to the inner wall of the second mounting plate, a first spring is fixedly connected to the outer walls on both sides of the limiting column, and the other end of the first spring is fixedly connected to the second mounting plate.

[0014] Preferably, the test tube clamping mechanism includes a sliding rod 1, a second gear, two clamping plates and two rack plates, one end of the sliding rod 1 is slidably connected to the limit column, the two clamping plates are slidably connected to the second mounting plate, the other end of the limit column is fixedly connected to one of the clamping plates, the two rack plates are meshed with the outer wall of the second gear, the two clamping plates are respectively fixedly connected to the two rack plates, the second gear is rotatably connected to the second mounting plate, and six test tube clamping mechanisms are provided, and the six test tube clamping mechanisms are distributed in a circular array.

[0015] Preferably, the extraction and separation mechanism includes a fourth mounting plate, a guide plate, an extraction motor, six first sliding rods, six extrusion blocks and six second sliding rods, the fourth mounting plate is fixedly connected to the fifth mounting plate, the extraction motor is fixedly connected to the fourth mounting plate, the output shaft of the extraction motor is fixedly connected to the guide plate, the six second sliding rods are all slidably connected to the fourth mounting plate, the six extrusion blocks are respectively fixedly connected to the second sliding rods, the six first sliding rods are all slidably connected to the guide plate, and the six first sliding rods are respectively fixedly connected to the six second sliding rods.

[0016] Preferably, the disinfection mechanism includes a rotating disk and a second fixed rod, the rotating disk is rotatably connected to the fifth mounting plate, the second fixed rod is fixedly connected to the output shaft of the extraction motor, the outer wall of the extraction motor is fixedly connected to a hexagonal rod, and the outer wall of the hexagonal rod is fixedly connected to six evenly distributed first racks.

[0017] Preferably, the closing mechanism includes a first bracket, a second pulley assembly, a first belt assembly, a closing disk, a first fixed rod, a first gear and two second springs, the first bracket is fixedly connected to the first mounting frame, the closing disk is rotatably connected to the first bracket via a set shaft, the two ends of the two second springs are respectively fixedly connected to the closing disk and the first mounting frame, the second pulley assembly is rotatably connected to the first bracket, the closing disk is fixedly connected to one end of the closing disk via a set shaft, the other end of the closing disk is fixedly connected to the first belt assembly, the other end of the first belt assembly is fixedly connected to the first gear via a set shaft, the first gear is rotatably connected to the first fixed rod via a set shaft, the first fixed rod is fixedly connected to the first mounting frame, and six closing mechanisms are provided, and the six closing mechanisms are distributed in a circular array.

[0018] Preferably, the wiper mechanism includes an arcuate slide, an arcuate rack, a stopper 1, a third spring, a fourth gear, a first bevel gear and a second bevel gear. The arcuate slide is fixedly connected to the first mounting bracket, the arcuate rack is slidably connected to the third spring, the stopper 1 is fixedly connected to the arcuate rack, the third spring is fixedly connected between the arcuate rack and the first mounting bracket, the fourth gear is meshed with the outer wall of the arcuate rack, the fourth gear is rotatably connected to the arcuate slide, the first bevel gear is fixedly connected to the first bevel gear via a set shaft, the outer wall of the first bevel gear is meshed with the second bevel gear, and the second bevel gear is rotatably connected to the arcuate slide.

[0019] Preferably, the scraping mechanism also includes a fourth shaft rod, a receiving groove, a third pulley assembly, a fifth shaft rod, two sixth gears, two third shaft rods and two first arc plates, the fourth shaft rod is fixedly connected to the second bevel gear, the fourth shaft rod is rotatably connected to the arc slide, the fourth shaft rod is fixedly connected to the fifth shaft rod, the fourth shaft rod is fixedly connected to the receiving groove, an end of the fifth shaft rod is fixedly connected to one end of the third pulley assembly, the other end of the third pulley assembly is fixedly connected to one of the third shaft rods, an outer wall of one end of the fifth shaft rod is fixedly connected to one of the sixth gears, the two sixth gears are meshed with each other, the other third shaft rod is fixedly connected to the other sixth gear, the ends of the two third shaft rods are respectively fixedly connected to the two first arc plates, and six scraping mechanisms are provided, and the six scraping mechanisms are distributed in a circular array.

[0020] Compared with the existing technology, the advantages of the separation device for culturing human stem cells are:

[0021] 1. When the first rack on the hexagonal rod is meshed with the first gear, the first gear drives the first belt assembly to rotate, and the first belt assembly drives the closing disk to open through the second pulley assembly. At this time, the closing disk contacts the outer wall of one side of the stem cell, so that the direction is flipped, so as to facilitate the extraction pipette to extend into the culture tube. Then, the extraction motor flips to drive the air bag on the extraction tube to contract and complete the extraction. At the same time, the second fixed rod drives the rotating disk to rotate. The outer wall of the rotating disk is provided with a disinfection cotton sheet. At the same time, the outer wall of the closing disk on the side in contact with the stem cell is cleaned and disinfected to avoid bacteria from being brought into the culture tube. At the same time, one closing disk corresponds to one culture tube, which will not cause confusion of experimental materials;

[0022] 2. Then, when the first rack meshes with the first gear again, the closing disk is driven to reset and close. At this time, the closing disk contacts the stopper 1, so that the stopper 1 drives the arc-shaped rack to move downward, thereby driving the fourth gear to rotate 180 degrees. The fourth gear drives the fourth shaft rod and the receiving groove to rotate through the first bevel gear and the second bevel gear, so that the receiving groove is located directly above the closing disk. At the same time, the fourth shaft rod can drive the two fourth gears to rotate in the opposite direction through the fifth shaft rod, the third pulley assembly and the sixth gear, thereby driving the two first arc plates to interlock. When the extraction test tube moves upward with the telescopic rod, the first arc plate can cause the liquid remaining on the outer wall of the extraction test tube to quickly fall into the receiving groove;

[0023] In summary, the present invention can clean and disinfect the outer wall of the closed disk on the side in contact with the stem cells during extraction and separation by cooperating with the disinfection mechanism through the extraction and separation mechanism, so as to avoid bringing bacteria into the culture tube. At the same time, one closed disk corresponds to one culture tube, which will not cause confusion of experimental materials. The switch of the closed mechanism can be controlled by the movement of the extraction and separation mechanism. At the same time, the closed mechanism cooperates with the scraping mechanism so that when the extraction tube moves up with the telescopic rod, the first arc plate can cause the liquid remaining on the outer wall of the extraction tube to fall quickly into the receiving groove without waiting time. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 It is a schematic diagram of the three-dimensional structure of the present invention;

[0025] Figure 2 The present invention Figure 1 A schematic diagram of the partially enlarged structure at center A;

[0026] Figure 3 It is a partial structural schematic diagram of the test tube clamping mechanism of the present invention;

[0027] Figure 4 The present invention Figure 3 A schematic diagram of the partially enlarged structure at B in the middle;

[0028] Figure 5 It is a structural schematic diagram of the limit column of the present invention;

[0029] Figure 6 The present invention Figure 5 A schematic diagram of the partially enlarged structure at C in the middle;

[0030] Figure 7 It is a partial structural schematic diagram of the extraction and separation mechanism of the present invention;

[0031] Figure 8 It is a partial structural schematic diagram of the closing mechanism of the present invention;

[0032] Fig. 9 The present invention Figure 8 The schematic diagram of the local enlarged structure at D in the middle;

[0033] Fig.10 It is a partial structural schematic diagram of the scraping mechanism of the present invention;

[0034] Fig.11 It is a schematic diagram of the structure of the third shaft rod of the present invention;

[0035] Fig.12 It is a schematic diagram of the structure of the disinfection mechanism of the present invention.

[0036] In the figure: 1, base; 2, telescopic rod; 3, guide plate; 4, first bearing frame; 5, first arc plate; 6, slide bar 1; 7, receiving groove; 8, first mounting frame; 9, second mounting plate; 10, knob assembly; 11, hexagonal rod; 12, first rack; 13, first belt assembly; 14, first gear; 15, first fixing rod; 16, closing plate; 17, clamping plate; 18, fixing column; 19, first shaft rod; 20, raised column; 21, centrifugal motor; 22, third mounting plate; 23, first spring; 24, limit column; 25, second shaft rod; 26, rack plate; 27, second gear; 28, first slide bar; 29, extrusion block; 30 , second slide bar; 31, extraction motor; 32, fourth mounting plate; 33, second spring; 34, second pulley assembly; 35, first bracket; 36, arc-shaped slide seat; 37, arc-shaped rack; 38, block one; 39, third spring; 40, fourth gear; 41, first bevel gear; 42, second bevel gear; 43, fourth shaft; 44, third shaft; 45, fifth shaft; 46, sixth gear; 47, third pulley assembly; 48, second fixed rod; 49, rotating disk; 50, fifth mounting plate; 101, test tube clamping mechanism; 202, extraction and separation mechanism; 303, sealing mechanism; 404, scraping mechanism; 505, disinfection mechanism. DETAILED DESCRIPTION

[0037] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0038] In the description of the present invention, it is necessary to understand that the terms "upper", "lower", "front", "back", "left", "right", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship are based on the orientation or position relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0039] Reference Figure 1-Figure 12A separation device for culturing human stem cells, comprising a base 1, a telescopic rod 2 is fixedly connected to the top outer wall of the base 1, a fifth mounting plate 50 is fixedly connected to the top outer wall of the telescopic rod 2, a first carrier frame 4 is movably connected to the outer wall of the fifth mounting plate 50, a centrifugal motor 21 is fixedly connected to the inner wall of the base 1, a third mounting plate 22 is fixedly connected to the output shaft of the centrifugal motor 21, two evenly distributed fixed columns 18 are fixedly connected to the top outer wall of the third mounting plate 22, a second mounting plate 9 is fixedly connected to the top outer wall of the two fixed columns 18, a first mounting frame 8 is fixedly connected to the top outer wall of the second mounting plate 9, and further comprising: a test tube clamping mechanism 101 for clamping a culture test tube, the test tube clamping mechanism 1 01 is installed on the second mounting plate 9; the extraction and separation mechanism 202 is used to squeeze the air bag of the extraction straw, and the extraction and separation mechanism 202 is installed on the fifth mounting plate 50; the closing mechanism 303 is used to cooperate with the movement of the extraction and separation mechanism 202 to close the culture test tube, and the closing mechanism 303 is installed on the first mounting frame 8; the scraping mechanism 404 is used to cooperate with the extraction and separation mechanism 202 so that the liquid on the outer wall of the extraction straw is accelerated to be discharged, and the scraping mechanism 404 is installed on the first mounting frame 8; and the disinfection mechanism 505 is used to disinfect the extraction and separation mechanism 202 when the extraction and separation mechanism 202 extracts stem cells, and the disinfection mechanism 505 is installed on the fifth mounting plate 50.

[0040] Among them, the inner wall of one of the fixed columns 18 is threadedly connected to the knob assembly 10, the inner wall of the knob assembly 10 is slidably connected to the second shaft rod 25, one end of the second shaft rod 25 is fixedly connected to the first shaft rod 19, the outer wall of the first shaft rod 19 is fixedly connected to the raised column 20, the inner wall of the second mounting plate 9 is slidably connected to the limiting column 24, the outer walls on both sides of the limiting column 24 are fixedly connected to the first spring 23, and the other end of the first spring 23 is fixedly connected to the second mounting plate 9.

[0041] Among them, the test tube clamping mechanism 101 includes a slide bar 6, a second gear 27, two clamping plates 17 and two rack plates 26, one end of the slide bar 6 is slidably connected to the limit column 24, the two clamping plates 17 are slidably connected to the second mounting plate 9, the other end of the limit column 24 is fixedly connected to one of the clamping plates 17, the two rack plates 26 are meshed with the outer wall of the second gear 27, the two clamping plates 17 are respectively fixedly connected to the two rack plates 26, the second gear 27 is rotatably connected to the second mounting plate 9, and six test tube clamping mechanisms 101 are provided, and the six test tube clamping mechanisms 101 are distributed in a circular array.

[0042] In this embodiment, the knob assembly 10 is rotated. Since a part of the knob assembly 10 is threadedly connected to the fixed column 18, the rotation angle of the protruding column 20 can be guaranteed not to change. The knob assembly 10 drives the protruding column 20 to rotate ninety degrees through the second shaft 25 and the first shaft 19, so that the protruding column 20 contacts the limit column 24 and drives the limit column 24 to move upward. Since the slide bar 6 slides along the limit column 24, the upward movement of the limit column 24 will drive the clamping plate 17 to move, and through the action of the two rack plates 26 and the second gear 27, the two clamping plates 17 are driven to clamp the culture test tube.

[0043] Among them, the extraction and separation mechanism 202 includes a fourth mounting plate 32, a guide plate 3, an extraction motor 31, six first slide bars 28, six extrusion blocks 29 and six second slide bars 30. The fourth mounting plate 32 is fixedly connected to the fifth mounting plate 50, the extraction motor 31 is fixedly connected to the fourth mounting plate 32, the output shaft of the extraction motor 31 is fixedly connected to the guide plate 3, the six second slide bars 30 are all slidably connected to the fourth mounting plate 32, the six extrusion blocks 29 are respectively fixedly connected to the second slide bars 30, the six first slide bars 28 are all slidably connected to the guide plate 3, and the six first slide bars 28 are respectively fixedly connected to the six second slide bars 30.

[0044] In this embodiment, the slide bar 6 is assumed to be in close contact with the culture tube, and then the centrifugal motor 21 rotates to drive the culture tube for centrifugation. After the centrifugation is completed, the extraction motor 31 rotates, so that the extraction motor 31 drives the guide plate 3 to rotate, so that the extrusion block 29 extends out to squeeze the air bag on the extraction tube.

[0045] Among them, the disinfection mechanism 505 includes a rotating disk 49 and a second fixed rod 48, the rotating disk 49 is rotatably connected to the fifth mounting plate 50, the second fixed rod 48 is fixedly connected to the output shaft of the extraction motor 31, the outer wall of the extraction motor 31 is fixedly connected to a hexagonal rod 11, and the outer wall of the hexagonal rod 11 is fixedly connected to six evenly distributed first racks 12.

[0046] Among them, the closing mechanism 303 includes a first bracket 35, a second pulley assembly 34, a first belt assembly 13, a closing disk 16, a first fixed rod 15, a first gear 14 and two second springs 33. The first bracket 35 is fixedly connected to the first mounting frame 8. The closing disk 16 is rotatably connected to the first bracket 35 through a set shaft rod. The two ends of the two second springs 33 are respectively fixedly connected to the closing disk 16 and the first mounting frame 8. The second pulley assembly 34 is rotatably connected to the first bracket 35. The closing disk 16 is fixedly connected to one end of the closing disk 16 through a set shaft rod. The other end of the closing disk 16 is fixedly connected to the first belt assembly 13. The other end of the first belt assembly 13 is fixedly connected to the first gear 14 through a set shaft rod. The first gear 14 is rotatably connected to the first fixed rod 15 through a set shaft rod. The first fixed rod 15 is fixedly connected to the first mounting frame 8. Six closing mechanisms 303 are provided, and the six closing mechanisms 303 are distributed in a circular array.

[0047] Among them, the telescopic rod 2 then moves down to start extracting the stem cell liquid. When the first rack 12 on the hexagonal rod 11 is meshed with the first gear 14, the first gear 14 drives the first belt assembly 13 to rotate, and the first belt assembly 13 drives the closing disk 16 to open through the second pulley assembly 34. At this time, the closing disk 16 contacts the outer wall of one side of the stem cell, so that the direction is flipped, thereby facilitating the extraction straw to extend into the culture tube. Subsequently, the extraction motor 31 flips to drive the airbag on the extraction tube to contract and complete the extraction. At the same time, the second fixed rod 48 drives the rotating disk 49 to rotate. The outer wall of the rotating disk 49 is provided with a disinfection cotton sheet. At the same time, the outer wall of the closing disk 16 on the side in contact with the stem cell is cleaned and disinfected to avoid bringing bacteria into the culture tube. At the same time, one closing disk 16 corresponds to one culture tube, which will not cause confusion of experimental materials.

[0048] Among them, the wiper mechanism 404 includes an arcuate slide 36, an arcuate rack 37, a stopper 38, a third spring 39, a fourth gear 40, a first bevel gear 41 and a second bevel gear 42. The arcuate slide 36 is fixedly connected to the first mounting frame 8, the arcuate rack 37 is slidably connected to the third spring 39, the stopper 38 is fixedly connected to the arcuate rack 37, the third spring 39 is fixedly connected between the arcuate rack 37 and the first mounting frame 8, the fourth gear 40 is meshed with the outer wall of the arcuate rack 37, the fourth gear 40 is rotatably connected to the arcuate slide 36, the first bevel gear 41 is fixedly connected to the first bevel gear 41 through a set shaft, the outer wall of the first bevel gear 41 is meshed with the second bevel gear 42, and the second bevel gear 42 is rotatably connected to the arcuate slide 36.

[0049] Among them, the scraping mechanism 404 also includes a fourth shaft rod 43, a receiving groove 7, a third pulley assembly 47, a fifth shaft rod 45, two sixth gears 46, two third shaft rods 44 and two first arc plates 5. The fourth shaft rod 43 is fixedly connected to the second bevel gear 42, the fourth shaft rod 43 is rotatably connected to the arc slide 36, the fourth shaft rod 43 is fixedly connected to the fifth shaft rod 45, the fourth shaft rod 43 is fixedly connected to the receiving groove 7, the end of the fifth shaft rod 45 is fixedly connected to one end of the third pulley assembly 47, the other end of the third pulley assembly 47 is fixedly connected to one of the third shaft rods 44, the outer wall of one end of the fifth shaft rod 45 is fixedly connected to one of the sixth gears 46, the two sixth gears 46 are meshed with each other, the other third shaft rod 44 is fixedly connected to the other sixth gear 46, the ends of the two third shaft rods 44 are respectively fixedly connected to the two first arc plates 5, and the scraping mechanism 404 is provided with six, and the six scraping mechanisms 404 are distributed in a circular array.

[0050] In this embodiment, when the first rack 12 is meshed with the first gear 14 again, it drives the closing disk 16 to reset and close. At this time, the closing disk 16 contacts the stopper 38, so that the stopper 38 drives the arc rack 37 to move downward, thereby driving the fourth gear 40 to rotate one hundred and eighty degrees. The fourth gear 40 drives the fourth shaft rod 43 and the receiving groove 7 to rotate through the first bevel gear 41 and the second bevel gear 42, so that the receiving groove 7 is located directly above the closing disk 16. At the same time, the fourth shaft rod 43 can drive the two fourth gears 40 to rotate in the opposite direction through the fifth shaft rod 45, the third pulley assembly 47 and the sixth gear 46, thereby driving the two first arc plates 5 to interlock. When the extraction test tube moves up with the telescopic rod 2, the first arc plate 5 can cause the liquid remaining on the outer wall of the extraction test tube to quickly fall into the receiving groove 7.

[0051] The specific working principle and method of use of the present invention are explained in detail below: Figure 1 The closing disk 16 in the figure is open. When the telescopic rod 2 is in the extended state during normal use, the first rack 12 and the first gear 14 are meshed with each other, and the closing disk 16 is closed. When in use, the extraction straw is first disinfected with a disinfection device, and then the knob assembly 10 needs to be rotated before the culture tube is centrifuged. Since a part of the knob assembly 10 is threadedly connected to the fixed column 18, it can be ensured that the rotation angle of the protruding column 20 will not change. The knob assembly 10 drives the protruding column 20 to rotate ninety degrees through the second shaft rod 25 and the first shaft rod 19, so that the protruding column 20 contacts the limit column 24 and drives the limit column 24 to move up. Since the slide bar 6 slides along the limit column 24, the upward movement of the limit column 24 will drive the clamping plate 17 to move, and the two rack plates 26 and the second gear 27 are used to drive the two clamping plates 17 to clamp the culture tube.

[0052] The default slider 6 is in close contact with the culture tube, and then the centrifugal motor 21 rotates to drive the culture tube for centrifugation. After the centrifugation is completed, the extraction motor 31 rotates, so that the extraction motor 31 drives the guide disk 3 to rotate, so that the extrusion block 29 extends out to squeeze the air bag on the extraction tube, and then the telescopic rod 2 moves down to start extracting the stem cell liquid. When the first rack 12 on the hexagonal rod 11 is meshed with the first gear 14, the first gear 14 drives the first belt assembly 13 to rotate, and the first belt assembly 13 drives the closing disk 16 to open through the second pulley assembly 34. At this time, the closing disk 16 contacts the outer wall of one side of the stem cell, so that the direction is flipped, so as to facilitate the extraction straw to extend into the culture tube. Then the extraction motor 31 flips to drive the air bag on the extraction tube to shrink and complete the extraction. At the same time, the second fixed rod 48 drives the rotating disk 49 to rotate. The outer wall of the rotating disk 49 is provided with a disinfection cotton sheet. At the same time, the outer wall of the closed disk 16 on the side in contact with the stem cell is cleaned and disinfected to avoid bacteria from being brought into the culture tube. At the same time, one closed disk 16 corresponds to one culture tube, which will not cause confusion of experimental materials.

[0053] Subsequently, when the first rack 12 meshes with the first gear 14 again, it drives the closing disk 16 to reset and close. At this time, the closing disk 16 contacts the stopper 38, so that the stopper 38 drives the arc rack 37 to move downward, thereby driving the fourth gear 40 to rotate one hundred and eighty degrees. The fourth gear 40 drives the fourth shaft rod 43 and the receiving groove 7 to rotate through the first bevel gear 41 and the second bevel gear 42, so that the receiving groove 7 is located directly above the closing disk 16. At the same time, the fourth shaft rod 43 can drive the two fourth gears 40 to rotate in the opposite direction through the fifth shaft rod 45, the third pulley assembly 47 and the sixth gear 46, thereby driving the two first arc plates 5 to interlock. When the extraction test tube moves up with the telescopic rod 2, the first arc plate 5 can cause the liquid remaining on the outer wall of the extraction test tube to quickly fall into the receiving groove 7.

[0054] At the same time, all extraction test tubes can be replaced at one time by removing the first carrier 4 from the top, which is very convenient and efficient.

[0055] It is further explained that the above-mentioned fixed connection should be understood in a broad sense unless otherwise clearly specified and limited. For example, it can be welding, gluing, or one-piece molding, etc., which are conventional means well known to those skilled in the art.

[0056] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A separation device for culturing human stem cells, comprising a base (1), a top outer wall of the base (1) being fixedly connected to a telescopic rod (2), a top outer wall of the telescopic rod (2) being fixedly connected to a fifth mounting plate (50), an outer wall of the fifth mounting plate (50) being movably connected to a first supporting frame (4), an inner wall of the base (1) being fixedly connected to a centrifugal motor (21), an output shaft of the centrifugal motor (21) being fixedly connected to a third mounting plate (22), a top outer wall of the third mounting plate (22) being fixedly connected to two evenly distributed fixing columns (18), a top outer wall of the two fixing columns (18) being fixedly connected to a second mounting plate (9), a top outer wall of the second mounting plate (9) being fixedly connected to a first mounting frame (8), characterized in that: Also includes: A test tube clamping mechanism (101), used for clamping a culture test tube, the test tube clamping mechanism (101) being mounted on the second mounting plate (9); An extraction and separation mechanism (202) is used to squeeze the air bag of the extraction straw, and the extraction and separation mechanism (202) is installed on the fifth installation plate (50); A sealing mechanism (303) for cooperating with the movement of the extraction and separation mechanism (202) to seal the culture tube, wherein the sealing mechanism (303) is mounted on the first mounting frame (8); a liquid scraping mechanism (404) for cooperating with the extraction and separation mechanism (202) so as to accelerate the discharge of liquid on the outer wall of the extraction straw, wherein the liquid scraping mechanism (404) is mounted on the first mounting frame (8); and a disinfection mechanism (505) for disinfecting the extraction and separation mechanism (202) when the extraction and separation mechanism (202) extracts stem cells. The disinfection mechanism (505) is installed on the fifth installation plate (50).

2. A separation device for culturing human stem cells according to claim 1, characterized in that: The inner wall of one of the fixed columns (18) is threadedly connected to a knob assembly (10), the inner wall of the knob assembly (10) is slidably connected to a second shaft rod (25), one end of the second shaft rod (25) is fixedly connected to a first shaft rod (19), the outer wall of the first shaft rod (19) is fixedly connected to a protruding column (20), the inner wall of the second mounting plate (9) is slidably connected to a limiting column (24), the outer walls on both sides of the limiting column (24) are fixedly connected to a first spring (23), and the other end of the first spring (23) is fixedly connected to the second mounting plate (9).

3. A separation device for culturing human stem cells according to claim 2, characterized in that: The test tube clamping mechanism (101) comprises a sliding rod (6), a second gear (27), two clamping plates (17) and two rack plates (26); one end of the sliding rod (6) is slidably connected to a limiting column (24); the two clamping plates (17) are both slidably connected to a second mounting plate (9); the other end of the limiting column (24) is fixedly connected to one of the clamping plates (17); the two rack plates (26) are both meshed with the outer wall of the second gear (27); the two clamping plates (17) are respectively fixedly connected to the two rack plates (26); the second gear (27) is rotatably connected to the second mounting plate (9); six test tube clamping mechanisms (101) are provided, and the six test tube clamping mechanisms (101) are distributed in a circular array.

4. A separation device for culturing human stem cells according to claim 3, characterized in that: The extraction and separation mechanism (202) comprises a fourth mounting plate (32), a guide plate (3), an extraction motor (31), six first slide bars (28), six extrusion blocks (29) and six second slide bars (30); the fourth mounting plate (32) is fixedly connected to the fifth mounting plate (50); the extraction motor (31) is fixedly connected to the fourth mounting plate (32); the output shaft of the extraction motor (31) is fixedly connected to the guide plate (3); the six second slide bars (30) are all slidably connected to the fourth mounting plate (32); the six extrusion blocks (29) are respectively fixedly connected to the second slide bars (30); the six first slide bars (28) are all slidably connected to the guide plate (3); and the six first slide bars (28) are respectively fixedly connected to the six second slide bars (30).

5. A separation device for culturing human stem cells according to claim 4, characterized in that: The disinfection mechanism (505) comprises a rotating disk (49) and a second fixed rod (48), wherein the rotating disk (49) is rotatably connected to the fifth mounting plate (50), and the second fixed rod (48) is fixedly connected to the output shaft of the extraction motor (31); the outer wall of the extraction motor (31) is fixedly connected to a hexagonal rod (11), and the outer wall of the hexagonal rod (11) is fixedly connected to six evenly distributed first racks (12).

6. A separation device for culturing human stem cells according to claim 5, characterized in that: The closing mechanism (303) comprises a first bracket (35), a second pulley assembly (34), a first belt assembly (13), a closing disk (16), a first fixing rod (15), a first gear (14) and two second springs (33); the first bracket (35) is fixedly connected to the first mounting frame (8); the closing disk (16) is rotatably connected to the first bracket (35) via a shaft; the two ends of the two second springs (33) are respectively fixedly connected to the closing disk (16) and the first mounting frame (8); the second pulley assembly (34) is fixedly connected to the first bracket (35) and the first mounting frame (8); The closure disk (16) is rotatably connected to one end of the closure disk (16) via a set shaft rod, the other end of the closure disk (16) is fixedly connected to the first belt assembly (13), the other end of the first belt assembly (13) is fixedly connected to the first gear (14) via a set shaft rod, the first gear (14) is rotatably connected to the first fixed rod (15) via a set shaft rod, the first fixed rod (15) is fixedly connected to the first mounting frame (8), and six closure mechanisms (303) are provided, and the six closure mechanisms (303) are distributed in a circular array.

7. A separation device for culturing human stem cells according to claim 6, characterized in that: The liquid scraping mechanism (404) comprises an arcuate slide (36), an arcuate rack (37), a stopper (38), a third spring (39), a fourth gear (40), a first bevel gear (41) and a second bevel gear (42); the arcuate slide (36) is fixedly connected to the first mounting frame (8); the arcuate rack (37) is slidably connected to the third spring (39); the stopper (38) is fixedly connected to the arcuate rack (37); the third spring (39) is fixedly connected between the arcuate rack (37) and the first mounting frame (8); the fourth gear (40) is meshed with an outer wall of the arcuate rack (37); the fourth gear (40) is rotationally connected to the arcuate slide (36); the first bevel gear (41) is fixedly connected to the first bevel gear (41) via a shaft rod; the outer wall of the first bevel gear (41) is meshed with the second bevel gear (42); and the second bevel gear (42) is rotationally connected to the arcuate slide (36).

8. A separation device for culturing human stem cells according to claim 7, characterized in that: The scraping mechanism (404) further comprises a fourth shaft (43), a receiving groove (7), a third pulley assembly (47), a fifth shaft (45), two sixth gears (46), two third shafts (44) and two first arc-shaped plates (5), wherein the fourth shaft (43) is fixedly connected to the second bevel gear (42), the fourth shaft (43) is rotationally connected to the arc-shaped slide seat (36), the fourth shaft (43) is fixedly connected to the fifth shaft (45), the fourth shaft (43) is fixedly connected to the receiving groove (7), and the end of the fifth shaft (45) is connected to the third One end of the pulley assembly (47) is fixedly connected, the other end of the third pulley assembly (47) is fixedly connected to one of the third shaft rods (44), the outer wall of one end of the fifth shaft rod (45) is fixedly connected to one of the sixth gears (46), the two sixth gears (46) are meshed with each other, the other third shaft rod (44) is fixedly connected to the other sixth gear (46), the ends of the two third shaft rods (44) are respectively fixedly connected to the two first arc-shaped plates (5), and the scraping mechanisms (404) are provided with six, and the six scraping mechanisms (404) are distributed in a circular array.

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