Anti-aging composition liposomes and methods of making and cosmetic use thereof

Abstract

The application provides an anti-aging composition liposome and a preparation method and cosmetics thereof, and relates to the technical field of biomedical materials.The anti-aging composition liposome comprises betaine, xylitol, a short-chain fatty alcohol, a phospholipid, an antioxidant and an anti-aging composition; the anti-aging composition comprises CutiFine CLR, CutiGuard CLR, a split yeast fermentation product lysate and oleuropein.The anti-aging composition liposome provided by the application adopts betaine and xylitol to form a deep eutectic solvent, has a good penetration-promoting effect, and improves the stability of the anti-aging composition by combining the liposome technology to synergistically promote penetration.The anti-aging composition liposome improves skin residence rate, effectively improves local treatment effect, reduces potential side effects, enhances the stability and transdermal absorption of active ingredients, and thus plays an important role in skin care and anti-aging treatment.

Description

Technical Field

[0001] This invention relates to the field of biomedical materials technology, and in particular to an anti-aging composition liposome, its preparation method, and cosmetics. Background Technology

[0002] The anti-aging composition includes CutiFine CLR, CutiGuard CLR, Bifida ferment lysate, and oleuropein. As a complex, it has relatively complex components and poor stability, and will settle after being left to stand for a long time. Moreover, since most of them are extracts or fermentation products, they are highly polar and therefore have difficulty penetrating the stratum corneum of the skin to exert their effects.

[0003] Liposomes are encapsulation systems formed by the self-assembly of phosphatidylcholine. They can carry both water-soluble and lipid-soluble components, allowing water-soluble components in anti-aging compositions to be loaded at their centers. The structure of liposomes is remarkably similar to that of cell membranes, exhibiting high biocompatibility and biodegradability, thus enabling them to carry anti-aging compositions through the stratum corneum of the skin to exert their effects.

[0004] Deep eutectic solvents are solvents formed by hydrogen bond acceptors and hydrogen bond donors. They share similar properties with ionic liquids that promote transdermal absorption, thus serving as a potential transdermal penetration enhancer. Betaine, an anti-inflammatory and anti-aging substance commonly used in cosmetics, is also a strong hydrogen bond acceptor capable of forming deep eutectic solvents with various hydrogen bond donors. It primarily enhances drug transdermal penetration by extracting lipids from the stratum corneum or altering the conformation of keratin. Existing technologies utilizing deep eutectic solvents to promote drug transdermal penetration mostly focus on facilitating the transdermal absorption of a single component or low-molecular-weight component. There is limited research on transdermal absorption of two or more target substances.

[0005] In view of this, the present invention is hereby proposed. Summary of the Invention

[0006] One of the objectives of this invention is to provide an anti-aging composition liposome to solve at least one of the aforementioned technical problems in the prior art.

[0007] The second objective of this invention is to provide a method for preparing an anti-aging composition liposome.

[0008] The third objective of this invention is to provide a cosmetic product.

[0009] In order to achieve the above-mentioned objectives of the present invention, the following technical solution is adopted:

[0010] The first aspect of the present invention provides an anti-aging composition liposome, comprising betaine, xylitol, short-chain fatty alcohol, phospholipid, antioxidant and anti-aging composition;

[0011] The anti-aging composition includes CutiFine CLR, CutiGuard CLR, Bifida ferment lysate, and oleuropein.

[0012] Furthermore, in the anti-aging composition, the mass ratio of CutiFine CLR, CutiGuard CLR, Bifida ferment lysate, and oleuropein is (0.5–3):(1–8):(0.5–3):(0.01–0.1), preferably 1:5:1:0.01–0.1.

[0013] Furthermore, the method for preparing the anti-aging composition is to mix CutiFine CLR, CutiGuard CLR, Bifida ferment lysate, and oleuropein evenly to obtain the anti-aging composition.

[0014] Further, the anti-aging composition liposomes comprise, by weight percentage, 0.3-5% betaine, 0.3-5% xylitol, 50-70% short-chain fatty alcohol, 3-8% phospholipids, 0.1-0.5% antioxidants, and 10-45% anti-aging composition.

[0015] Further, the short-chain fatty alcohol includes at least one of ethanol, 1,2-propanediol, 1,3-propanediol, glycerol, butanediol, pentanediol, and hexanediol, preferably 1,3-propanediol and glycerol.

[0016] Furthermore, the phospholipids include soybean lecithin and / or hydrogenated lecithin;

[0017] Preferably, the antioxidant includes sodium metabisulfite, sodium thiosulfate, L-cysteine, and glutathione.

[0018] A second aspect of the present invention provides a method for preparing the liposomes of the anti-aging composition, comprising the following steps:

[0019] A. Mix betaine, xylitol and some short-chain fatty alcohols evenly, then add phospholipids and mix evenly to obtain a mixed solution;

[0020] B. Mix the remaining short-chain fatty alcohol, antioxidant and anti-aging composition evenly, add to the mixed solution to obtain a homogeneous solution, and finally homogenize the homogeneous solution to obtain the anti-aging composition liposomes.

[0021] Furthermore, in step A, the mixing temperature is 50–80°C.

[0022] Preferably, the mixture is added to the solution while it is being stirred.

[0023] Preferably, the stirring speed is 300-400 rpm and the stirring time is 5-15 min.

[0024] Furthermore, the pressure of the homogenization is 15,000 to 25,000 psi.

[0025] Preferably, the homogenization cycle is repeated 1 to 5 times.

[0026] Preferably, the discharge temperature after homogenization is 10–30°C.

[0027] A third aspect of the present invention provides a cosmetic product comprising the anti-aging composition liposomes described in the first aspect.

[0028] Compared with the prior art, the present invention has at least the following beneficial effects:

[0029] The anti-aging composition liposomes provided by this invention utilize betaine and xylitol to form a deep eutectic solvent, exhibiting excellent penetration-enhancing effects. This is achieved through synergistic penetration enhancement using liposome technology. Water-soluble substances in the anti-aging composition are dispersed in the hydrophilic centers of the liposomes, while lipid-soluble substances are dispersed within the liposome membrane. The presence of liposomes significantly improves the stability of the anti-aging composition. In vitro transdermal experiments show that the skin retention of these anti-aging composition liposomes is increased by approximately 7 times. Furthermore, the improved skin retention rate of these liposomes effectively enhances local therapeutic effects, reduces potential side effects, and strengthens the stability and transdermal absorption of active ingredients, thus playing a crucial role in skin care and anti-aging treatments.

[0030] The method for preparing anti-aging composition liposomes provided by the present invention involves betaine and xylitol forming a deep eutectic solvent during the preparation of a mixed solution, followed by liposome preparation technology. This combination of two processes improves the solubility, stability, and subsequent bioavailability of the liposomes.

[0031] The cosmetic provided by this invention, in view of the advantages of the above-mentioned anti-aging composition liposomes, improves the anti-aging effect of the prepared cosmetic, and also improves the safety, stability and transdermal absorption capacity of the product, providing consumers with an efficient, safe and widely applicable anti-aging option. Detailed Implementation

[0032] The embodiments and examples of the present invention will be described in detail below with reference to the implementation methods and examples. However, those skilled in the art will understand that the following implementation methods and examples are only for illustrating the present invention and should not be regarded as limiting the scope of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0033] The first aspect of the present invention provides an anti-aging composition liposome, comprising betaine, xylitol, short-chain fatty alcohol, phospholipid, antioxidant and anti-aging composition;

[0034] The anti-aging composition includes CutiFine CLR, CutiGuard CLR, Bifida ferment lysate, and oleuropein.

[0035] In this anti-aging composition, CutiFine CLR acts on the dermal compartments surrounding hair follicles, strengthening the skin's structural support and thus maintaining small pores. CutiGuard CLR is a cell aging reversal factor that prevents premature skin aging by reducing the number of senescent cells and the inflammatory and destructive mediators associated with visibly aging skin, while also reducing wrinkles. Bifida ferment lysate promotes precise DNA repair and prevents DNA damage caused by UV radiation, providing surface protection for the skin. Oleuropein also protects cells from UV damage and prevents the breakdown of the skin membrane by UV rays. Therefore, Bifida ferment lysate and oleuropein in this anti-aging composition act as the first line of defense against skin aging, preventing cell aging by avoiding UV damage. CutiFine CLR and CutiGuard CLR then exert deeper anti-aging effects on cells through repair, anti-inflammation, and anti-oxidation. These substances work together to promote anti-aging and anti-aging of the skin, achieving a better overall anti-aging effect.

[0036] The main active ingredients in CutiFine CLR are Vaccaria segetalis extract and baobab fruit pulp extract. Vaccaria segetalis extract contains saponins, flavonoids, polysaccharides, and cyclic peptides, which have anti-inflammatory, antioxidant, anti-angiogenic, and even anti-tumor effects. Baobab fruit pulp extract mainly contains high levels of calcium and vitamin C, as well as sugars, triterpenes, saponins, and riboflavin.

[0037] CutiFine CLR can improve the problem of enlarged pores by reducing skin secretions, reducing glycation, eliminating free radicals, reducing inflammation, and strengthening the dermal structure.

[0038] CutiGuard CLR is a cell aging reversal factor. Its main active ingredients are betaine and hydrolyzed red algae extract, which can prevent premature skin aging by reducing the number of senescent cells and the inflammatory and destructive mediators associated with visibly aging skin, while also reducing wrinkles.

[0039] Bifida ferment lysate can promote precise DNA repair and prevent DNA damage caused by ultraviolet radiation, thus protecting the skin's surface. Oleuropein also protects cells from ultraviolet damage and prevents the breakdown of the skin membrane by ultraviolet rays. Therefore, the Bifida ferment lysate and oleuropein in the anti-aging composition act as the first line of defense against skin aging, preventing cell aging by avoiding ultraviolet damage. CutiFineCLR and CutiGuard CLR, through repair, anti-inflammation, and antioxidant mechanisms, exert anti-aging effects on cells at a deeper level, working together to promote skin anti-aging and anti-aging.

[0040] Furthermore, the mass ratio of CutiFine CLR, CutiGuard CLR, Bifida ferment lysate and oleuropein is (0.5-3):(1-8):(0.5-3):(0.01-0.1), preferably 1:5:1:0.01-0.1.

[0041] Typically, but not limitingly, the mass ratio of CutiFine CLR, CutiGuard CLR, Bifida ferment lysate, and oleuropein can be 0.5:1:0.5:0.01, 1:2:1:0.02, 2:6:1.5:0.05, 3:8:3:0.1, or any other ratio within the range of (0.5–3):(1–8):(0.5–3):(0.01–0.1); the preferred ratio range can be 1:5:1:0.01, 1:5:1:0.02, 1:5:1:0.03, 1:5:1:0.04, 1:5:1:0.05, 1:5:1:0.06, 1:5:1:0.07, 1:5:1:0.08, or 1:5:1:0.09, or any value within the range of 1:5:1:0.01–0.1.

[0042] The method for preparing the anti-aging composition is to mix CutiFine CLR, CutiGuard CLR, Bifida ferment lysate, and oleuropein evenly to obtain the anti-aging composition.

[0043] The method for preparing the anti-aging composition provided by this invention is simple, involves mixing all raw materials evenly, and allows for large batch processing capacity, making it suitable for large-scale industrial production.

[0044] Anti-aging compositions contain a variety of active ingredients, which are complex, unstable, highly hydrophilic, and not easily absorbed by the human stratum corneum. Therefore, by using liposome technology to encapsulate water-soluble ingredients in the center of liposomes, both stability and transdermal absorption can be improved.

[0045] The anti-aging composition liposomes provided by this invention disperse water-soluble substances in the hydrophilic centers of the liposomes, while lipid-soluble substances are dispersed within the liposome membrane. The presence of the liposomes significantly enhances the stability of the anti-aging composition. In vitro transdermal experiments show that the skin retention of these anti-aging composition liposomes is increased by approximately 7 times. Furthermore, these liposomes improve skin retention, effectively enhance local treatment efficacy, reduce potential side effects, and strengthen the stability and transdermal absorption of active ingredients, thus playing a crucial role in skin care and anti-aging treatments.

[0046] Furthermore, the anti-aging composition liposomes comprise, by weight percentage, 0.3-5% betaine, 0.3-5% xylitol, 50-70% short-chain fatty alcohols, 3-8% phospholipids, 0.1-0.5% antioxidants, and 10-45% anti-aging composition.

[0047] Typical, but not limiting, the mass percentage of betaine in the liposomes of the anti-aging composition can be 0.3%, 0.5%, 1%, 2%, 3%, 4%, or 5%, or any value within the range of 0.3% to 5%; the mass percentage of xylitol can be 0.3%, 0.5%, 1%, 2%, 3%, 4%, or 5%, or any value within the range of 0.3% to 5%; the mass percentage of short-chain fatty alcohols can be 50%, 52%, 54%, 56%, 58%, 60%, 62%, 64%, 66%, 68%, or 70%, or any value within the range of 50% to 70%; the mass percentage of phospholipids... The mass percentage of the antioxidant can be 3%, 3.5%, 4%, 4.5%, 5%, 5.5%, 6%, 7%, or 8%, or any value within the range of 3% to 8%; the mass percentage of the antioxidant can be 0.1%, 0.2%, 0.3%, 0.4%, or 0.5%, or any value within the range of 0.1% to 0.5%; the mass percentage of the anti-aging composition can be 10%, 12%, 14%, 16%, 18%, 20%, 22%, 24%, 26%, 28%, 30%, 32%, 34%, 36%, 38%, 40%, or 45%, or any value within the range of 10% to 45%.

[0048] Further, the short-chain fatty alcohol includes at least one of ethanol, 1,2-propanediol, 1,3-propanediol, glycerol, butanediol, pentanediol, and hexanediol, preferably 1,3-propanediol and glycerol.

[0049] Preferably, the phospholipids include soybean lecithin and / or hydrogenated lecithin.

[0050] Preferably, the antioxidant includes sodium metabisulfite, sodium thiosulfate, L-cysteine, and glutathione.

[0051] A second aspect of the present invention provides a method for preparing the liposomes of the anti-aging composition, comprising the following steps:

[0052] A. Mix betaine, xylitol and some short-chain fatty alcohols evenly, then add phospholipids and mix evenly to obtain a mixed solution;

[0053] B. Mix the remaining short-chain fatty alcohol, antioxidant and anti-aging composition evenly, add to the mixed solution to obtain a homogeneous solution, and finally homogenize the homogeneous solution to obtain the anti-aging composition liposomes.

[0054] The present invention provides a method for preparing liposomes of an anti-aging composition. During the preparation of the mixed solution, betaine and xylitol form a deep eutectic solvent, followed by liposome preparation technology. This combination of two processes improves the solubility, stability, and subsequent bioavailability of the liposomes. The presence of the deep eutectic solvent alters the structure of stratum corneum cells, facilitating the fusion of liposome particles with keratin lipids, thereby aiding in the delivery of active substances to the dermis, promoting liposome penetration, and ultimately enhancing the anti-aging effect of the liposomes.

[0055] Liposomes are encapsulation systems formed by the self-assembly of phosphatidylcholine. They can carry both water-soluble and lipid-soluble components, allowing water-soluble components in anti-aging compositions to be loaded at their centers. The structure of liposomes is remarkably similar to that of cell membranes, exhibiting high biocompatibility and biodegradability, thus enabling them to carry anti-aging compositions through the stratum corneum of the skin to exert their effects.

[0056] Deep eutectic solvents are solvents formed by hydrogen bond acceptors and hydrogen bond donors. They share similar properties with ionic liquids that promote transdermal absorption, thus serving as a good transdermal penetration enhancer. Betaine, an anti-inflammatory and anti-aging substance commonly used in cosmetics, is also a strong hydrogen bond acceptor capable of forming deep eutectic solvents with various hydrogen bond donors. It primarily enhances drug transdermal penetration by extracting lipids from the stratum corneum or altering the conformation of keratin. This invention utilizes liposome technology for synergistic penetration enhancement.

[0057] Furthermore, in step A, the mixing temperature is 50–80°C.

[0058] Typically, but not limitingly, the mixing temperature in step A can be, for example, 50°C, 55°C, 60°C, 65°C, 70°C, 75°C, or 80°C, or any value within the range of 50°C to 80°C.

[0059] Preferably, the mixture is added to the solution while it is being stirred.

[0060] Preferably, the stirring speed is 300-400 rpm and the stirring time is 5-15 min.

[0061] Typically, but not limitingly, the stirring speed can be, for example, 300 rpm, 320 rpm, 340 rpm, 360 rpm, 380 rpm, or 400 rpm, or any value within the range of 300 to 400 rpm; the stirring time can be, for example, 5 min, 7 min, 9 min, 11 min, 13 min, or 15 min, or any value within the range of 5 to 15 min.

[0062] Furthermore, the pressure of the homogenization is 15,000 to 25,000 psi.

[0063] Typically, but not limitingly, the homogeneous pressure can be, for example, 15,000 psi, 18,000 psi, 21,000 psi, 24,000 psi, 27,000 psi, or any value in the range of 15,000 to 25,000 psi.

[0064] Preferably, the homogenization cycle is repeated 1 to 5 times.

[0065] Typically, but not limitingly, the number of homogeneous cycles can be, for example, 1, 2, 3, 4, or 5 times, or any value within the range of 1 to 5 times.

[0066] Preferably, the discharge temperature after homogenization is 10–30°C.

[0067] Typically, but not limitingly, the discharge temperature after homogenization can be, for example, 10°C, 15°C, 20°C, 25°C, or 30°C, or any value within the range of 10°C to 30°C.

[0068] A third aspect of the present invention provides a cosmetic product comprising the aforementioned anti-aging composition liposomes.

[0069] The cosmetic provided by this invention, in view of the advantages of the above-mentioned anti-aging composition liposomes, improves the anti-aging effect of the prepared cosmetic, and also improves the safety, stability and transdermal absorption capacity of the product, providing consumers with an efficient, safe and widely applicable anti-aging option.

[0070] The present invention is further illustrated below with specific embodiments and comparative examples. However, it should be understood that these embodiments are merely for illustrative purposes and should not be construed as limiting the invention in any way. Unless otherwise specified, the raw materials used in the embodiments and comparative examples of the present invention were carried out under conventional conditions or conditions recommended by the manufacturer. Reagents or instruments used, unless otherwise specified, are all commercially available conventional products.

[0071] Example 1

[0072] This embodiment provides an anti-aging composition, which is obtained by uniformly mixing CutiFine CLR (CLRBerlin, Germany), CutiGuard CLR (CLR Berlin, Germany), Bifida ferment lysate and oleuropein in a mass ratio of 1:5:1:0.05.

[0073] Example 2

[0074] This embodiment provides an anti-aging liposome composition, which is composed of the following components:

[0075] The composition contains 20% 1,3-propanediol, 0.5% betaine, 0.9% xylitol, 5% soybean lecithin, 38.3% glycerol, 35.2% of the anti-aging composition of Example 1, and 0.1% sodium metabisulfite.

[0076] The specific preparation method is as follows:

[0077] 1. Stir betaine, xylitol and 1,3-propanediol at 50-80℃ until the solution becomes transparent, then add soybean lecithin and stir at 65℃ until dissolved and transparent, then cool to 50℃ to obtain a mixed solution.

[0078] 2. Stir glycerol, the anti-aging composition, and sodium metabisulfite until they are completely mixed and free of particles. Then slowly add them to the mixed solution at a speed of 400 rpm for 10 minutes to obtain a homogeneous solution.

[0079] 3. Homogenize the homogenized solution using a homogenizer, controlling the homogenization pressure at 20,000 psi, the number of cycles at 3, and the discharge temperature at 20℃ to obtain the anti-aging composition liposomes.

[0080] Example 3

[0081] This embodiment provides an anti-aging liposome composition, which is composed of the following components:

[0082] The composition contains 20% 1,3-propanediol, 0.5% betaine, 0.3% xylitol, 5% soybean lecithin, 38.9% glycerol, 35.2% of the anti-aging composition of Example 1, and 0.1% sodium metabisulfite.

[0083] The preparation method is the same as that in Example 2.

[0084] Example 4

[0085] This embodiment provides an anti-aging liposome composition, which is composed of the following components:

[0086] The composition contains 20% 1,3-propanediol, 0.5% betaine, 5% xylitol, 5% soybean lecithin, 34.2% glycerol, 35.2% of the anti-aging composition of Example 1, and 0.1% sodium metabisulfite.

[0087] The preparation method is the same as that in Example 2.

[0088] Example 5

[0089] This embodiment provides an anti-aging liposome composition, which is composed of the following components:

[0090] The composition contains 20% 1,3-propanediol, 0.5% betaine, 0.9% xylitol, 5% soybean lecithin, 63.5% glycerol, 10% of the anti-aging composition of Example 1, and 0.1% sodium metabisulfite.

[0091] The preparation method is the same as that in Example 2.

[0092] Example 6

[0093] This embodiment provides an anti-aging liposome composition, which is composed of the following components:

[0094] The composition contains 20% 1,3-propanediol, 0.5% betaine, 0.9% xylitol, 5% soybean lecithin, 28.5% glycerol, 45% of the anti-aging composition of Example 1, and 0.1% sodium metabisulfite.

[0095] The preparation method is the same as that in Example 2.

[0096] Example 7

[0097] This embodiment provides an anti-aging liposome composition, which is composed of the following components:

[0098] The composition contains 20% 1,3-propanediol, 0.5% betaine, 0.9% xylitol, 5% soybean lecithin, 64.5% glycerol, 9% of the anti-aging composition of Example 1, and 0.1% sodium metabisulfite.

[0099] The preparation method is the same as that in Example 2.

[0100] Example 8

[0101] This embodiment provides an anti-aging liposome composition, which is composed of the following components:

[0102] The composition contains 20% 1,3-propanediol, 0.5% betaine, 0.9% xylitol, 5% soybean lecithin, 27.5% glycerol, 46% of the anti-aging composition of Example 1, and 0.1% sodium metabisulfite.

[0103] The preparation method is the same as that in Example 2.

[0104] Comparative Example 1

[0105] This comparative example provides an anti-aging composition emulsion, which is composed of the following components:

[0106] The composition contained 20% 1,3-propanediol, 44.7% glycerol, 35.2% of the anti-aging composition of Example 1, and 0.1% sodium metabisulfite.

[0107] The preparation method is as follows:

[0108] Glycerol and the anti-aging composition were mixed and stirred until transparent to obtain an anti-aging composition solution; then 1,3-propanediol and sodium metabisulfite were mixed evenly to obtain a sodium metabisulfite solution; finally, the sodium metabisulfite solution and the anti-aging composition solution were mixed evenly and homogenized under high pressure at a pressure of 15000 psi for 4 times to obtain an anti-aging composition emulsion.

[0109] Comparative Example 2

[0110] This comparative example provides an anti-aging composition emulsion, which is composed of the following components:

[0111] The composition contained 20% 1,3-propanediol, 43.3% glycerol, 35.2% of the anti-aging composition of Example 1, 0.1% sodium metabisulfite, 0.5% betaine, and 0.9% xylitol.

[0112] The preparation method is as follows:

[0113] Glycerol and the anti-aging composition were mixed and stirred until transparent to obtain an anti-aging composition solution. Then, 1,3-propanediol, betaine, xylitol and sodium metabisulfite were stirred at 70°C until transparent to obtain a mixed solution containing a deep eutectic solvent. Finally, the mixed solution and the anti-aging composition solution were mixed evenly and subjected to high-pressure homogenization at a pressure of 15,000 psi for 4 times to obtain an anti-aging composition emulsion.

[0114] Comparative Example 3

[0115] This comparative example provides an anti-aging composition liposome, which is composed of the following components:

[0116] The composition contained 20% 1,3-propanediol, 39.7% glycerol, 35.2% of the anti-aging composition of Example 1, 0.1% sodium metabisulfite, and 5% soybean lecithin.

[0117] The preparation method is as follows:

[0118] Glycerol and the anti-aging composition were mixed and stirred until transparent to obtain an anti-aging composition solution. Then, 1,3-propanediol, soybean lecithin and sodium metabisulfite were stirred at 80°C for 30 minutes until transparent, and then cooled to 50°C to obtain a mixed solution. Finally, the mixed solution and the anti-aging composition solution were mixed evenly and homogenized under high pressure at a pressure of 15000 psi for 4 times to obtain anti-aging composition liposomes.

[0119] Test Example 1

[0120] The liposomes or emulsions obtained in the examples and comparative examples were subjected to dynamic light scattering detection to measure their average particle size after 30 days, and their appearance after 30 days was observed. The results are recorded in Table 1.

[0121] Table 1

[0122] Sample Name 30d particle size Appearance changes Example 2 546.3 Stable, no crystallization Example 3 544.1 Stable, no crystallization Example 4 623.4 Turbid, with a small number of small crystals Example 5 526.5 Stable, no crystallization Example 6 728.1 Turbidity, crystallization Example 7 522.7 Stable, no crystallization Example 8 805.6 Layering, crystallization Comparative Example 1 677.3nm Slightly cloudy, no crystals Comparative Example 2 654.3nm Slightly cloudy, no crystallization Comparative Example 3 558.4nm Stable, no crystallization

[0123] Test Example 2

[0124] The permeability of the liposomes or emulsions obtained in the examples and comparative examples was compared using the following method:

[0125] Using oleuropein as a standard, 50 parts of liposomes or emulsions were weighed and then mixed with 50 parts of SIMULGELFL (1.6%) (Seppic, France) aqueous solution at 300 r / min to obtain the required essence for testing.

[0126] Skin retention rate test: Transdermal validation was performed according to the in vitro transdermal testing guidelines specified in the OECD / OCDE Guidelines for Chemical Testing.

[0127] The dwell time data is recorded in Table 2.

[0128] Table 2

[0129]

[0130] Comparative analysis of Tables 1 and 2 shows that the retention of the sample solutions from Comparative Examples 1, 2, and 3 in the skin was significantly lower than that of the liposomes prepared in Example 2, with the highest retention reaching 2.04 μg / cm³. 2 .

[0131] Comparative analysis of Examples 1-3 shows that the transdermal absorption of oleuropein can be improved by introducing xylitol cocrystal solvent and liposome encapsulation technology, with the cocrystal solvent showing a slightly better penetration-enhancing effect than liposome treatment. Example 2, obtained by incorporating the cocrystal solvent obtained from xylitol and betaine treatment into liposomes, exhibits approximately 7 times the penetration-enhancing effect on oleuropein emulsion compared to the untreated oleuropein emulsion, a 3.8-fold increase compared to the xylitol cocrystal solvent, and a 4.7-fold increase compared to oleuropein liposomes. Therefore, this method, combining xylitol cocrystal solvent with liposome encapsulation technology, can significantly improve the skin retention of oleuropein.

[0132] Using oleuropein in the anti-aging composition as a standard, a penetration comparison experiment was conducted. As shown in Table 2, when the xylitol content was 0.9% in Example 2, the deep co-crystal liposome composition formed with betaine exhibited good stability and significantly enhanced the retention of oleuropein in the skin. When the xylitol content was significantly reduced to 0.3%, the retention of oleuropein in the stratum corneum of the skin decreased significantly. When the xylitol content was 5%, the excessive hydrogen bond donors led to saturation of the co-crystal solvent, and the excessive xylitol also affected the stability of the liposomes, resulting in a slightly lower transdermal transdermal absorption of oleuropein in the anti-aging composition compared to Example 2. Therefore, it can be considered that the xylitol addition of 0.9% in Example 2 is the optimal preparation scheme while ensuring economy, stability, and efficiency.

[0133] The presence of the anti-aging composition affects the morphology of liposomes. When the content of the anti-aging composition is low, the morphology and integrity of the liposomes remain stable, as in Examples 5 and 7. However, due to the low content of the anti-aging composition, the skin retention is also low. As the content of the anti-aging composition increases, the skin retention also increases. However, when it increases to a higher level, the skin retention decreases instead. This is because higher levels of the anti-aging composition can disrupt the morphology of the liposomes, thus affecting their stability and transdermal efficacy. Studies have shown that when the content of the anti-aging composition is around 35.2%, both its stability and transdermal performance are at a better level, as described in Example 2.

[0134] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. An anti-aging composition liposome, characterized by, Composed of: 1,3-propanediol 20%, betaine 0.5%, xylitol 0.9%, soybean lecithin 5%, glycerol 38.3%, anti-aging composition 35.2%, sodium metabisulfite 0.1%; Wherein the anti-aging composition is obtained by uniformly mixing CutiFine CLR, CutiGuard CLR, Saccharomyces Lycium Ferment Lysate and Oleuropein with a mass ratio of 1:5:1:0.05; The specific preparation method is as follows: S1, stir betaine, xylitol and 1,3-propanediol at 50-80℃ until the solution becomes transparent, then add soybean lecithin, stir at 65℃ until dissolved and transparent, then cool to 50℃ to obtain a mixed solution; S2, stir glycerol, anti-aging composition and sodium metabisulfite until completely mixed without particulate matter, then slowly add the mixed solution, set the rotation speed to 400rpm and the time to 10min to obtain a uniform solution; S3, use a homogenizer to homogenize the uniform solution, control the homogenization pressure to 20000psi, the cycle number to 3 times and the discharge temperature to 20℃ to obtain the anti-aging composition liposome.

2. A cosmetic product, characterized by, The anti-aging composition liposome of claim 1.

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