Cell picture shooting method and device, electronic equipment and storage medium

Abstract

The invention relates to the field of medicine, and discloses a cell picture shooting method, a cell picture shooting device, electronic equipment and a computer readable storage medium. The cell picture shooting method comprises the steps that at least two first cell pictures are acquired, the at least two first cell pictures are cell pictures shot under different first shooting parameters, and the light intensity values of the at least two first cell pictures are different; constructing a first correlation function of the first shooting parameter and the light intensity value according to the first cell picture; and substituting the target light intensity value into the first correlation function, determining a target first shooting parameter corresponding to the target light intensity, and shooting a target cell picture based on the target first shooting parameter. Compared with the prior art, the cell picture shooting method, the cell picture shooting device, the electronic equipment and the computer readable storage medium provided by the embodiment of the invention have the advantages of improving the reliability of a cell shooting result while improving the cell shooting efficiency.

Description

Technical Field

[0001] The present application relates to the field of medicine, and more specifically, to a method for taking cell images, a device for taking cell images, an electronic device, and a computer-readable storage medium. Background Art

[0002] Cells are living organisms that make up various tissues and organs, and cell health directly affects human health. Whether in the process of pharmaceutical research and development or in the actual process of disease inspection and treatment, processes such as cell counting and morphological analysis play a vital role. In traditional technologies, processes such as cell counting and morphological analysis require staff to observe cells with the naked eye under a microscope, but currently more electronic instruments such as cell counters and cell analyzers are used to achieve processes such as cell counting and morphological analysis. The working principle of electronic instruments such as cell counters and cell analyzers is to take cell pictures, and then perform image analysis on the taken cell pictures to achieve processes such as cell counting and morphological analysis. In this process, the shooting effect of the cell pictures greatly affects the final results of cell counting and morphological analysis.

[0003] However, in the related art, when taking cell pictures, the light intensity requirements for the cell pictures are different due to the influence of factors such as different application scenarios, different detection instruments, and different cell types. In order to obtain a cell picture that meets the light intensity requirements, it is necessary to continuously adjust the shooting parameters and perform multiple cell shots, which seriously affects the cell shooting efficiency. In addition, for some types of cells, continuous exposure during multiple cell shots may also stimulate cell fluorescence, causing the cells to produce "phototoxicity" and "photobleaching", resulting in irreversible fluorescence quenching, which reduces the reliability of the cell shooting results. Summary of the invention

[0004] The purpose of the present application is to provide a cell image taking method, a cell image taking device, an electronic device and a computer-readable storage medium, which can improve the efficiency of cell imaging while improving the reliability of cell imaging results.

[0005] In a first aspect, an embodiment of the present application provides a method for taking a cell image, comprising: obtaining at least two first cell images, wherein the at least two first cell images are cell images taken under different first shooting parameters, and the light intensity values ​​of the at least two first cell images are different; constructing a first correlation function between the first shooting parameter and the light intensity value based on the first cell images; substituting the target light intensity value into the first correlation function, determining the target first shooting parameter corresponding to the target light intensity, taking a first reference cell image based on the target first shooting parameter, and using the first reference cell image as the target cell image corresponding to the target light intensity value.

[0006] Compared with the related art, in the cell image shooting method provided in the embodiment of the present application, after constructing the first correlation function of the first shooting parameter and the light intensity value through at least two first cell images, the corresponding target first shooting parameter can be directly determined according to the target light intensity value, and the cell image can be subsequently shot based on the target first shooting parameter. Compared with the related art of first adjusting the shooting parameters for shooting, and then selecting the image that meets the target light intensity value requirement from the shooting results of multiple shots, in the present embodiment, directly determining the target first shooting parameter for shooting the cell image according to the target light intensity value can reduce the number of cell image shooting times and improve the cell shooting efficiency; at the same time, due to the reduction in the number of cell image shooting times, the number of cell exposure times is also reduced, and the impact of exposure on the cells is also smaller, so the reliability of the cell shooting results can also be improved.

[0007] In an optional embodiment, before using the first reference cell image as the target cell image corresponding to the target light intensity value, the cell image shooting method further includes: repeatedly executing the first detection scheme; the first detection scheme includes: judging whether the first light intensity deviation value between the actual light intensity value of the first reference cell image and the target light intensity value is greater than the first preset deviation threshold; if the first light intensity deviation value is greater than the first preset deviation threshold, updating the first correlation function based on the target first shooting parameter and the actual light intensity value, substituting the target light intensity value into the updated first correlation function, determining the new target first shooting parameter corresponding to the target light intensity, and shooting a new first reference cell image based on the new target first shooting parameter; if the first light intensity deviation value is less than or equal to the first preset deviation threshold, ending the first detection scheme. After actually shooting the first reference cell image, verifying the actually shot first reference cell image by the first preset deviation threshold can further improve the reliability of the cell shooting result; at the same time, updating the first correlation function according to the actually shot first reference cell image can make the first correlation function more in line with the actual application scenario and improve the reliability of the updated first correlation function.

[0008] In an optional embodiment, the first shooting parameter is exposure duration, and constructing a first correlation function between the first shooting parameter and the light intensity value based on the first cell image includes: constructing a first coordinate system of the first shooting parameter and the light intensity value, and performing linear fitting on the at least two first cell images in the first coordinate system to obtain the first correlation function.

[0009] In an optional embodiment, before taking the first reference cell picture based on the target shooting parameter, the cell picture shooting method further includes: determining whether the target first shooting parameter is greater than a first preset parameter threshold; if the target first shooting parameter is greater than the first preset parameter threshold, obtaining at least two second cell pictures, the at least two second cell pictures are cell pictures taken under different second shooting parameters, and the at least two second cell pictures have different light intensity values; constructing a second correlation function between the second shooting parameter and the light intensity value according to the second cell picture; substituting the target light intensity value into the second correlation function, determining the target second shooting parameter corresponding to the target light intensity, and reacquiring the first cell picture based on the target second shooting parameter. If the target first shooting parameter is greater than the first preset parameter threshold, it means that there may be problems with other shooting parameters of the first cell picture. At this time, the other shooting parameters, i.e., the second shooting parameters, are adjusted, and the first cell picture is reacquired based on the adjusted target second shooting parameters, so that the adjustment of multiple different shooting parameters can be achieved, and the reliability of the cell shooting results can be further improved.

[0010] In an optional embodiment, before reacquiring the first cell image based on the target second shooting parameter, the cell image shooting method further includes: repeatedly executing the second detection scheme; the second detection scheme includes: shooting a second reference cell image based on the target second shooting parameter; judging whether the second light intensity deviation value between the actual light intensity value of the second reference cell image and the target light intensity value is greater than the second preset deviation threshold; if the second light intensity deviation value is greater than the second preset deviation threshold, updating the second correlation function based on the target second shooting parameter and the actual light intensity value, substituting the target light intensity value into the updated second correlation function, and determining the new target second shooting parameter corresponding to the target light intensity; if the second light intensity deviation value is less than or equal to the second preset deviation threshold, terminating the second detection scheme. After actually shooting the second reference cell image, verifying the actually shot second reference cell image by the second preset deviation threshold can further improve the reliability of the cell shooting result; at the same time, updating the second correlation function according to the actually shot second reference cell image can make the second correlation function more in line with the actual application scenario and improve the reliability of the updated second correlation function.

[0011] In an optional embodiment, the second shooting parameter is the magnification corresponding to the exposure gain, and constructing a second correlation function between the second shooting parameter and the light intensity value based on the second cell image includes: constructing a second coordinate system for the second shooting parameter and the light intensity value, and performing linear fitting on the at least two second cell images in the second coordinate system to obtain the second correlation function.

[0012] In an optional embodiment, the first shooting parameter is exposure duration, and the acquisition of at least two first cell images includes: selecting at least two first shooting parameters that are less than a second preset parameter threshold, and obtaining the at least two first cell images by shooting based on the at least two first shooting parameters, each of the first cell images corresponds to a unique first shooting parameter, and the second preset parameter threshold is less than the first preset parameter threshold. Obtaining at least two first cell images by actual shooting can make the first correlation function more in line with actual application scenarios and improve the reliability of the first correlation function; at the same time, the second preset parameter threshold is less than the first preset parameter threshold, that is, the exposure duration when shooting the first cell image is short, which can reduce the exposure duration when shooting the first cell image and reduce the impact of the exposure process on the cell.

[0013] In a second aspect, an embodiment of the present application provides a cell image shooting device, comprising: a sample acquisition module, the sample acquisition module is used to acquire at least two first cell images, the at least two first cell images are cell images taken under different first shooting parameters, and the light intensity values ​​of the at least two first cell images are different; a function construction module, the function construction module is used to construct a first correlation function between the first shooting parameter and the light intensity value according to the first cell image; a parameter determination module, the parameter determination module is used to substitute the target light intensity value into the first correlation function, and determine the target first shooting parameter corresponding to the target light intensity; a shooting module, the shooting module is used to shoot a first reference cell image based on the target first shooting parameter, and use the first reference cell image as the target cell image corresponding to the target light intensity value.

[0014] Compared with the related art, in the cell image shooting device provided in the embodiment of the present application, after acquiring at least two first cell images through the sample acquisition module, the function construction module can construct a first correlation function between the first shooting parameter and the light intensity value according to the at least two first cell images, and then the parameter determination module can directly determine the corresponding target first shooting parameter according to the target light intensity value, and the subsequent shooting module can shoot the cell image based on the target first shooting parameter. Compared with the related art of first adjusting the shooting parameters for shooting, and then selecting the image that meets the target light intensity value requirement from the shooting results of multiple shots, in this embodiment, directly determining the target first shooting parameter for shooting the cell image according to the target light intensity value can reduce the number of cell image shooting times and improve the cell shooting efficiency; at the same time, due to the reduction in the number of cell image shooting times, the number of exposure times of the cell is also reduced, and the impact of exposure on the cell is also smaller, so the reliability of the cell shooting results can also be improved.

[0015] In a third aspect, an embodiment of the present application provides an electronic device, comprising: at least one processor; and a memory communicatively connected to the at least one processor; wherein the memory stores instructions executable by the at least one processor, and the instructions are executed by the at least one processor so that the at least one processor can execute the cell image taking method as described above.

[0016] In a fourth aspect, an embodiment of the present application provides a computer-readable storage medium storing a computer program, wherein the computer program is executed by a processor to implement the cell image taking method as described above. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required for use in the embodiments will be briefly introduced below. It should be understood that the following drawings only show certain embodiments of the present application and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other related drawings can also be obtained based on these drawings.

[0018] Figure 1 This is a schematic diagram of the process of the cell image taking method provided in Example 1 of the present application; Figure 2 This is a schematic diagram of the function of exposure time and light intensity value in the cell image shooting method provided in Example 1 of the present application; Figure 3 This is a schematic diagram of the process of the cell image taking method provided in Example 2 of the present application; Figure 4 This is a schematic diagram of the process of the cell image taking method provided in Example 3 of the present application; Figure 5 This is a schematic diagram of the function of magnification and light intensity in the cell image shooting method provided in Example 3 of the present application; Figure 6 This is a schematic diagram of the process of the cell image taking method provided in Example 4 of the present application; Figure 7 This is a schematic diagram of the structure of a cell image taking device provided in Example 5 of the present application; Figure 8 A schematic diagram of the structure of a cell image taking device provided in another embodiment of the present application; Fig. 9 This is a schematic diagram of the structure of an electronic device provided in Example 6 of the present application. DETAILED DESCRIPTION

[0019] In order to make the purpose, technical solution and advantages of the embodiments of the present application clearer, the technical solution in the embodiments of the present application will be described in detail below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, rather than all the embodiments. The components of the embodiments of the present application described and shown in the drawings here can be arranged and designed in various different configurations.

[0020] Therefore, the following detailed description of the embodiments of the present application provided in the accompanying drawings is not intended to limit the scope of the present application as claimed, but is merely representative of selected embodiments of the present application.

[0021] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, further definition and explanation thereof is not required in subsequent drawings.

[0022] In addition, the terms “first”, “second”, etc., if used, are merely used to distinguish between the descriptions and should not be understood as indicating or implying relative importance.

[0023] It should be noted that, in the absence of conflict, the features in the embodiments of the present application may be combined with each other.

[0024] Embodiment 1 The first embodiment of the present application provides a method for taking a cell picture, such as Figure 1 As shown, the following steps are included: Step S101: Acquire at least two first cell images.

[0025] Specifically, in this step, at least two first cell images correspond to different first shooting parameters and light intensity values, that is, each first cell image corresponds to a first shooting parameter and a light intensity value, and the first shooting parameters corresponding to at least two first cell images in this step are different, and the corresponding light intensity values ​​are also different.

[0026] The first shooting parameter is a parameter related to shooting the first cell image, and in this embodiment, the first shooting parameter is exposure time. It can be understood that the first shooting parameter being exposure time is a specific example of the first shooting parameter in this embodiment, and in some other embodiments of the present application, the first shooting parameter may also be other parameters such as exposure gain.

[0027] In this embodiment, obtaining at least two first cell images may specifically be actually taking at least two first cell images. Correspondingly, at least two first shooting parameters are selected, and then the cells are photographed based on the at least two first shooting parameters, and the shooting results are at least two first cell images. Obtaining at least two first cell images by actually shooting can make the first correlation function more in line with actual application scenarios and improve the reliability of the first correlation function.

[0028] Further, in combination with the aforementioned first shooting parameter being the exposure time, when actually shooting at least two first cell images, at least two selected first shooting parameters are less than the second preset parameter threshold, and the second preset parameter threshold is less than the first preset parameter threshold. The second preset parameter threshold is less than the first preset parameter threshold, that is, the exposure time when shooting the first cell image is shorter, which can reduce the exposure time when shooting the first cell image and reduce the impact of the exposure process on the cell.

[0029] Among them, the first preset parameter threshold and the second preset parameter threshold are two pre-set threshold values ​​corresponding to the first shooting parameter. When the first shooting parameter is greater than the first preset parameter threshold, the corresponding cell image is invalid. Conversely, when the first shooting parameter is less than or equal to the first preset parameter threshold, the corresponding cell image is valid; the second preset parameter threshold is a pre-set threshold value that is less than the first preset parameter threshold.

[0030] It can be understood that the aforementioned acquisition of at least two first cell images is merely a specific example of actually taking at least two first cell images in the present embodiment. In some embodiments of the present application, the acquisition of at least two first cell images may also be, for example, acquiring at least two historical cell images from stored historical cell images as first cell images, or establishing a communication connection with a server, a host computer, or other devices, and receiving at least two first cell images sent by a server, a host computer, or other devices.

[0031] Step S102: constructing a first correlation function between a first shooting parameter and a light intensity value according to the first cell image.

[0032] In this step, the first correlation function can be constructed by image fitting, specifically, a first coordinate system of the first shooting parameters and the light intensity values ​​is constructed, and the points corresponding to the first cell images are determined in the first coordinate system according to the first shooting parameters and the light intensity values ​​of the first cell images. Then, image fitting is performed on the points corresponding to all the first cell images to obtain the first correlation function of the first shooting parameters and the light intensity values.

[0033] Specifically, in this embodiment, the first shooting parameter is combined with the exposure time, such as Figure 2As shown, there is a linear relationship between the exposure time and the light intensity value. When constructing the first correlation function by image fitting, linear fitting can be performed on points corresponding to at least two first cell images in the first coordinate system to obtain the first correlation function.

[0034] Step S103: Substitute the target light intensity value into the first correlation function to determine the target first shooting parameter corresponding to the target light intensity.

[0035] In this step, the target light intensity is substituted into the first correlation function, and the target first shooting parameter corresponding to the target light intensity can be calculated according to the first correlation function.

[0036] Step S104: taking a first reference cell picture based on a target first shooting parameter.

[0037] In this step, a first reference cell picture is taken based on the target first shooting parameter, that is, a cell picture whose first shooting parameter is the target first shooting parameter is taken as the first reference cell picture.

[0038] Step S105: taking the first reference cell image as the target cell image corresponding to the target light intensity value.

[0039] Compared with the related art, in the cell image shooting method provided in Example 1 of the present application, after constructing the first correlation function of the first shooting parameter and the light intensity value through at least two first cell images, the corresponding target first shooting parameter can be directly determined according to the target light intensity value, and then the cell image can be shot based on the target first shooting parameter. Compared with the related art of first adjusting the shooting parameters for shooting, and then selecting the image that meets the target light intensity value requirement from the shooting results of multiple shots, in this embodiment, directly determining the target first shooting parameter for shooting the cell image according to the target light intensity value can reduce the number of cell image shooting times and improve the cell shooting efficiency; at the same time, due to the reduction in the number of cell image shooting times, the number of exposure times of the cell is also reduced, and the impact of exposure on the cell is also smaller, so the reliability of the cell shooting results can also be improved.

[0040] Embodiment 2 Embodiment 2 of the present application provides a method for taking cell images, such as Figure 3 As shown, the following steps are included: Step S201: Acquire at least two first cell images.

[0041] Step S202: constructing a first correlation function between a first shooting parameter and a light intensity value according to the first cell image; Step S203: Substitute the target light intensity value into the first correlation function to determine the target first shooting parameter corresponding to the target light intensity.

[0042] Step S204: taking a first reference cell picture based on a first target shooting parameter.

[0043] It can be understood that steps S201 to S204 in the second embodiment are substantially the same as steps S101 to S104 in the first embodiment, and the details may refer to the specific description in the first embodiment.

[0044] Step S205: Determine whether a first light intensity deviation value between an actual light intensity value of the first reference cell image and a target light intensity value is greater than a first preset deviation threshold value; if so, execute step S206; if not, execute step S209.

[0045] In this embodiment, after the first reference cell image is captured, the first reference cell image is analyzed to obtain the actual light intensity value of the first reference cell image, and then the actual light intensity value is subtracted from the target light intensity value to obtain the first light intensity deviation value. It can be understood that the first light intensity deviation value is the difference between the actual light intensity value and the target light intensity value, which is always a positive value. Specifically, for example, the absolute value of the difference between the actual light intensity value and the target light intensity value can be used as the first light intensity deviation value.

[0046] Since there is an error between theoretical calculation and actual shooting, a first preset deviation threshold can be set to verify the first reference cell image. The first preset deviation threshold is pre-set and can accept a large error value. When the first light intensity deviation value is less than or equal to the first preset deviation threshold, it is verified that the first reference cell image meets the light intensity requirements and can be used as a target cell image corresponding to the target light intensity value. At this time, step S209 is executed; conversely, when the first light intensity deviation value is greater than the first preset deviation threshold, it is verified that the first reference cell image does not meet the light intensity requirements and cannot be used as a target cell image corresponding to the target light intensity value. At this time, step S206 is executed.

[0047] Step S206: updating the first correlation function based on the first shooting parameter of the target and the actual light intensity value.

[0048] In this step, points corresponding to the first reference cell image can be determined in the first coordinate system based on the target first shooting parameter and the actual light intensity value, and then image fitting is performed again on all points in the first coordinate system to obtain an updated first correlation function.

[0049] Step S207: Substitute the target light intensity value into the adjusted first correlation function to determine a new target first shooting parameter corresponding to the target light intensity.

[0050] Step S208: taking a new first reference cell picture based on the new target first shooting parameter.

[0051] In this embodiment, after re-shooting to obtain a new first reference cell image, step S205 can be executed again in a loop to determine whether the first light intensity deviation value between the actual light intensity value of the new first reference cell image and the target light intensity value is greater than the first preset deviation threshold. That is, steps S205 to S208 are repeatedly executed until the first light intensity deviation value is less than or equal to the first preset deviation threshold, and step S209 is executed. The repeated execution of steps S205 to S208 is the first detection scheme.

[0052] Step S209: taking the first reference cell image as the target cell image corresponding to the target light intensity value.

[0053] Compared with the related art, in the cell image shooting method provided in Example 2 of the present application, after the first reference cell image is actually shot, the actually shot first reference cell image is verified by a first preset deviation threshold, which can further improve the reliability of the cell shooting results; at the same time, the first correlation function is adjusted according to the actually shot first reference cell image, which can make the first correlation function more in line with the actual application scenario, thereby improving the reliability of the adjusted first correlation function.

[0054] Embodiment 3 Embodiment 3 of the present application provides a method for taking cell pictures, such as Figure 4 As shown, the following steps are included: Step S301: Acquire at least two first cell images.

[0055] Step S302: constructing a first correlation function between a first shooting parameter and a light intensity value according to the first cell image; Step S303: Substitute the target light intensity value into the first correlation function to determine the target first shooting parameter corresponding to the target light intensity.

[0056] It can be understood that steps S301 to S303 in the third embodiment are substantially the same as steps S101 to S103 in the first embodiment, and the details may refer to the specific description in the first embodiment.

[0057] Step S304: Determine whether the first shooting parameter of the target is greater than the first preset parameter threshold, if so, execute step S305, if not, execute step S309.

[0058] In this step, the first preset parameter threshold is a pre-set threshold value corresponding to the first shooting parameter. When the target first shooting parameter is greater than the first preset parameter threshold, the corresponding cell image is invalid, and step S305 is executed. On the contrary, when the target first shooting parameter is less than or equal to the first preset parameter threshold, the corresponding cell image is valid, and step S309 is executed.

[0059] Step S305: Acquire at least two second cell images.

[0060] In this step, at least two second cell images correspond to different second shooting parameters and light intensity values, that is, each second cell image corresponds to a second shooting parameter and a light intensity value. The second shooting parameters corresponding to at least two second cell images in this step are different, and the corresponding light intensity values ​​are also different.

[0061] Among them, the second shooting parameter is a relevant parameter when shooting the second cell picture. In this embodiment, the second shooting parameter is the magnification corresponding to the exposure gain. In this embodiment, there is a corresponding relationship between the exposure gain and the magnification of "magnification = 10^(gain / 20)". It can be understood that the second shooting parameter is the magnification corresponding to the exposure gain, which is a specific example of the second shooting parameter in this embodiment. In some other embodiments of the present application, the second shooting parameter can also be other parameters such as exposure time. For example, when the first shooting parameter is the exposure time, the second shooting parameter can be the magnification corresponding to the exposure gain; when the first shooting parameter is the magnification corresponding to the exposure gain, the second shooting parameter can be the exposure time.

[0062] In this embodiment, obtaining at least two second cell images may specifically be actually taking at least two second cell images. Correspondingly, at least two second shooting parameters are selected, and then the cells are photographed based on the at least two second shooting parameters, and the shooting results are at least two second cell images. Obtaining at least two second cell images by actually shooting can make the second correlation function more in line with actual application scenarios and improve the reliability of the second correlation function.

[0063] It can be understood that the aforementioned acquisition of at least two second cell images is merely a specific example of actually taking at least two second cell images in the present embodiment. In some embodiments of the present application, the acquisition of at least two second cell images can also be, for example, acquiring at least two historical cell images from stored historical cell images as second cell images, or establishing a communication connection with a server, a host computer, or other devices, and receiving at least two second cell images sent by a server, a host computer, or other devices.

[0064] Step S306: constructing a second correlation function between a second shooting parameter and a light intensity value according to the second cell image.

[0065] In this step, the second correlation function can be constructed by image fitting. Specifically, a second coordinate system of the second shooting parameters and the light intensity values ​​is constructed. According to the second shooting parameters and the light intensity values ​​of each second cell image, the points corresponding to each second cell image are determined in the second coordinate system. Then, image fitting is performed on the points corresponding to all the second cell images to obtain the second correlation function of the second shooting parameters and the light intensity values.

[0066] Specifically, in this embodiment, the second shooting parameter is combined with the exposure time, such as Figure 5 As shown, there is a linear relationship between the exposure time and the light intensity value. When constructing the second correlation function by image fitting, linear fitting can be performed on the points corresponding to at least two second cell images in the second coordinate system to obtain the second correlation function.

[0067] Step S307: Substitute the target light intensity value into the second correlation function to determine the target second shooting parameter corresponding to the target light intensity.

[0068] Step S308: reacquire the first cell image based on the target second shooting parameter.

[0069] In this step, reacquiring the first cell image means acquiring at least two first cell images whose second shooting parameters are the target second shooting parameters. The specific steps are substantially the same as acquiring at least two first cell images in step S101. For details, please refer to the specific description in the aforementioned step S101.

[0070] Furthermore, after obtaining the first cell image in this step, step S302 may be executed again.

[0071] Step S309: taking a first reference cell picture based on the target first shooting parameter.

[0072] Step S310: taking the first reference cell image as the target cell image corresponding to the target light intensity value.

[0073] Compared with the related art, in the cell image shooting method provided in the third embodiment, the target first shooting parameter is greater than the first preset parameter threshold, which means that there may be problems with other shooting parameters of the first cell image. At this time, the other shooting parameters, that is, the second shooting parameters, are adjusted, and the first cell image is reacquired based on the adjusted target second shooting parameters. This can achieve adjustment of a variety of different shooting parameters, further improving the reliability of the cell shooting results.

[0074] Embodiment 4 Embodiment 4 of the present application provides a method for taking cell images, such as Figure 6 As shown, the following steps are included: Step S401: Acquire at least two first cell images.

[0075] Step S402: constructing a first correlation function between a first shooting parameter and a light intensity value according to the first cell image; Step S403: Substitute the target light intensity value into the first correlation function to determine the target first shooting parameter corresponding to the target light intensity.

[0076] It can be understood that steps S401 to S403 in the third embodiment are substantially the same as steps S101 to S103 in the first embodiment, and the details may refer to the specific description in the first embodiment.

[0077] Step S404: Determine whether the first shooting parameter of the target is greater than the first preset parameter threshold, if so, execute step S405, if not, execute step S409.

[0078] Step S405: Acquire at least two second cell images.

[0079] Step S406: constructing a second correlation function between a second shooting parameter and a light intensity value according to the second cell image.

[0080] Step S407: Substitute the target light intensity value into the second correlation function to determine the target second shooting parameter corresponding to the target light intensity.

[0081] Step S408: taking a second reference cell picture based on the target second shooting parameter.

[0082] In this step, a second reference cell picture is taken based on the target second shooting parameter, that is, a cell picture whose second shooting parameter is the target second shooting parameter is taken as the second reference cell picture.

[0083] Step S409: Determine whether a second light intensity deviation value between the actual light intensity value of the second reference cell image and the target light intensity value is greater than a second preset deviation threshold value, if so, execute step S410, if not, execute step S412.

[0084] In this embodiment, after the second reference cell image is captured, the second reference cell image is analyzed to obtain the actual light intensity value of the second reference cell image, and then the second light intensity deviation value is obtained by performing a difference operation on the actual light intensity value and the target light intensity value. It can be understood that the second light intensity deviation value is the difference between the actual light intensity value and the target light intensity value, which is always a positive value. Specifically, for example, the absolute value of the difference between the actual light intensity value and the target light intensity value can be used as the second light intensity deviation value.

[0085] Since there is an error between theoretical calculation and actual shooting, a second preset deviation threshold can be set to verify the second reference cell image. The second preset deviation threshold is pre-set and can accept a large error value. When the second light intensity deviation value is less than or equal to the second preset deviation threshold, it is verified that the second reference cell image meets the light intensity requirement, and step S412 is executed at this time; conversely, when the second light intensity deviation value is greater than the second preset deviation threshold, it is verified that the second reference cell image does not meet the light intensity requirement, and step S410 is executed at this time.

[0086] Step S410: adjusting the second correlation function based on the target second shooting parameter and the actual light intensity value.

[0087] In this step, points corresponding to the second reference cell image can be determined in the second coordinate system based on the target second shooting parameter and the actual light intensity value, and then image fitting is performed again on all points in the second coordinate system to obtain an updated second correlation function.

[0088] Step S411: Substitute the target light intensity value into the adjusted second correlation function to determine a new target second shooting parameter corresponding to the target light intensity.

[0089] Step S412: reacquire the first cell image based on the target second shooting parameter.

[0090] Step S413: taking a first reference cell picture based on the target first shooting parameter.

[0091] Step S414: taking the first reference cell image as the target cell image corresponding to the target light intensity value.

[0092] Compared with the related art, in the cell image shooting method provided in the fourth embodiment, after the second reference cell image is actually shot, the second reference cell image actually shot is verified by a second preset deviation threshold, which can further improve the reliability of the cell shooting results; at the same time, the second correlation function is adjusted according to the second reference cell image actually shot, which can make the second correlation function more in line with the actual application scenario, thereby improving the reliability of the adjusted second correlation function.

[0093] Embodiment 5 Embodiment 5 of the present application relates to a cell image taking device, such as Figure 7As shown, it includes: a sample acquisition module 101, the sample acquisition module 101 is used to acquire at least two first cell images, at least two first cell images are cell images taken under different first shooting parameters, and at least two first cell images have different light intensity values; a function construction module 102, the function construction module 102 is used to construct a first correlation function between the first shooting parameter and the light intensity value according to the first cell image; a parameter determination module 103, the parameter determination module 103 is used to substitute the target light intensity value into the first correlation function, and determine the target first shooting parameter corresponding to the target light intensity; a shooting module 104, the shooting module 104 is used to shoot a first reference cell image based on the target first shooting parameter, and use the first reference cell image as the target cell image corresponding to the target light intensity value.

[0094] Compared with the related art, in the cell image shooting device provided in Example 5 of the present application, after at least two first cell images are acquired by the sample acquisition module 101, the function construction module 102 can construct a first correlation function between the first shooting parameter and the light intensity value according to the at least two first cell images, and then the parameter determination module 103 can directly determine the corresponding target first shooting parameter according to the target light intensity value, and the subsequent shooting module 104 can shoot the cell image based on the target first shooting parameter. Compared with the related art of first adjusting the shooting parameters for shooting, and then selecting the image that meets the target light intensity value requirement from the shooting results of multiple shots, in this embodiment, directly determining the target first shooting parameters for shooting the cell image according to the target light intensity value can reduce the number of cell image shooting times and improve the cell shooting efficiency; at the same time, due to the reduction in the number of cell image shooting times, the number of exposure times to the cell is also reduced, and the impact of exposure on the cell is also smaller, so the reliability of the cell shooting results can also be improved.

[0095] It can be understood that, based on the specific instructions in the aforementioned step S101, in some embodiments of the present application, obtaining at least two first cell images can specifically be actually taking at least two first cell images. In this embodiment, the sample acquisition module 101 can be replaced by the shooting module 104, that is, in this embodiment, the cell image shooting device includes only: a function construction module 102, a parameter determination module 103 and a shooting module 104, wherein the shooting module 104 is not only used to take a first reference cell image based on the target first shooting parameter, but also used to obtain at least two first cell images.

[0096] In some embodiments of the present application, Figure 8As shown, the cell image shooting device can also include a judgment module 105, which is used to judge whether a first light intensity deviation value between an actual light intensity value of a first reference cell image and a target light intensity value is greater than a first preset deviation threshold value; the function construction module 102 is also used to update a first correlation function based on a target first shooting parameter and an actual light intensity value when the first light intensity deviation value is greater than the first preset deviation threshold value, the parameter determination module 103 is also used to substitute the target light intensity value into the updated first correlation function, and determine a new target first shooting parameter corresponding to the target light intensity, and the shooting module 104 is also used to shoot a new first reference cell image based on the new target first shooting parameter.

[0097] In some embodiments of the present application, the judgment module 105 is also used to judge whether the target first shooting parameter is greater than the first preset parameter threshold; the sample acquisition module 101 is also used to obtain at least two second cell images when the target first shooting parameter is greater than the first preset parameter threshold, at least two second cell images are cell images taken under different second shooting parameters, and at least two second cell images have different light intensity values; the function construction module 102 is also used to construct a second correlation function between the second shooting parameter and the light intensity value based on the second cell image; the parameter determination module 103 is also used to substitute the target light intensity value into the second correlation function to determine the target second shooting parameter corresponding to the target light intensity, and the sample acquisition module 101 is also used to re-acquire the first cell image based on the target second shooting parameter.

[0098] In some embodiments of the present application, the shooting module 104 is also used to shoot a second reference cell image based on the target second shooting parameter; the judgment module 105 is also used to judge whether the second light intensity deviation value between the actual light intensity value of the second reference cell image and the target light intensity value is greater than the second preset deviation threshold; the function construction module 102 is also used to update the second correlation function based on the target second shooting parameter and the actual light intensity value when the second light intensity deviation value is greater than the second preset deviation threshold, and the parameter determination module 103 is also used to substitute the target light intensity value into the updated second correlation function to determine the new target second shooting parameter corresponding to the target light intensity.

[0099] In some embodiments of the present application, the parameter determination module 103 is also used to select at least two first shooting parameters that are less than a second preset parameter threshold, and the shooting module 104 is also used to obtain at least two first cell images based on the at least two first shooting parameters, and the second preset parameter threshold is less than the first preset parameter threshold.

[0100] Embodiment 6 Embodiment 6 of the present application relates to an electronic device, such as Fig. 9As shown, it includes: at least one processor 201; and a memory 202 that is communicatively connected to the at least one processor 201; wherein the memory 202 stores instructions that can be executed by the at least one processor 201, and the instructions are executed by the at least one processor 201 so that the at least one processor 201 can execute the cell image taking method in the above-mentioned embodiments.

[0101] Among them, the memory and the processor are connected in a bus manner, and the bus may include any number of interconnected buses and bridges, and the bus connects various circuits of one or more processors and memories together. The bus can also connect various other circuits such as peripherals, voltage regulators, and power management circuits, which are well known in the art and are therefore not further described herein. The bus interface provides an interface between the bus and the transceiver. The transceiver can be one element or multiple elements, such as multiple receivers and transmitters, providing a unit for communicating with various other devices on a transmission medium. The data processed by the processor is transmitted on a wireless medium via an antenna, and further, the antenna also receives data and transmits the data to the processor.

[0102] The processor is responsible for managing the bus and general processing, and can also provide various functions, including timing, peripheral interfaces, voltage regulation, power management, and other control functions. Memory can be used to store data used by the processor when performing operations.

[0103] Embodiment 7 Embodiment 7 of the present application relates to a computer-readable storage medium storing a computer program. When the computer program is executed by a processor, the above method embodiment is implemented.

[0104] That is, those skilled in the art can understand that all or part of the steps in the above-mentioned embodiment method can be completed by instructing the relevant hardware through a program, and the program is stored in a storage medium, including several instructions to enable a device (which can be a single-chip microcomputer, chip, etc.) or a processor to execute all or part of the steps of each embodiment method of the present application. The aforementioned storage medium includes: U disk, mobile hard disk, read-only memory (ROM, Read-Only Memory), random access memory (RAM, Random Access Memory), disk or optical disk and other media that can store program codes.

[0105] The above are only specific implementations of the present application, but the protection scope of the present application is not limited thereto. Any changes or substitutions that can be easily thought of by a person skilled in the art within the technical scope disclosed in the present application should be included in the protection scope of the present application. Therefore, the protection scope of the present application shall be based on the protection scope of the claims.

Claims

1. A method for taking cell pictures, characterized in that: include: Acquire at least two first cell images, wherein the at least two first cell images are cell images taken under different first shooting parameters, and the at least two first cell images have different light intensity values; Constructing a first correlation function between a first shooting parameter and a light intensity value according to the first cell picture; Substitute the target light intensity value into the first correlation function, determine the target first shooting parameter corresponding to the target light intensity, take a first reference cell picture based on the target first shooting parameter, and use the first reference cell picture as the target cell picture corresponding to the target light intensity value.

2. The cell image shooting method according to claim 1, characterized in that: Before using the first reference cell image as the target cell image corresponding to the target light intensity value, the cell image shooting method further includes: Repeat the first detection plan; The first detection scheme includes: Determine whether a first light intensity deviation value between an actual light intensity value of the first reference cell image and the target light intensity value is greater than a first preset deviation threshold; If the first light intensity deviation value is greater than the first preset deviation threshold, updating the first correlation function based on the target first shooting parameter and the actual light intensity value, substituting the target light intensity value into the updated first correlation function, determining a new target first shooting parameter corresponding to the target light intensity, and taking a new first reference cell image based on the new target first shooting parameter; If the first light intensity deviation value is less than or equal to the first preset deviation threshold, the first detection scheme is terminated.

3. The cell image shooting method according to claim 1, characterized in that: The first shooting parameter is exposure time, and the first correlation function between the first shooting parameter and the light intensity value is constructed according to the first cell image, including: A first coordinate system of first shooting parameters and light intensity values ​​is constructed, and linear fitting is performed on the at least two first cell images in the first coordinate system to obtain the first correlation function.

4. The cell image shooting method according to claim 1, characterized in that: Before taking the first reference cell picture based on the target shooting parameters, the cell picture taking method further includes: Determining whether a first shooting parameter of the target is greater than a first preset parameter threshold; If the target first shooting parameter is greater than the first preset parameter threshold, obtaining at least two second cell images, wherein the at least two second cell images are cell images taken under different second shooting parameters, and the light intensity values ​​of the at least two second cell images are different; Constructing a second correlation function between a second shooting parameter and a light intensity value according to the second cell picture; Substitute the target light intensity value into the second correlation function to determine a target second shooting parameter corresponding to the target light intensity, and reacquire the first cell image based on the target second shooting parameter.

5. The cell image shooting method according to claim 4, characterized in that: Before reacquiring the first cell image based on the target second shooting parameter, the cell image shooting method further includes: Repeat the second detection scheme; The second detection scheme includes: Taking a second reference cell picture based on the second shooting parameter of the target; Determine whether a second light intensity deviation value between an actual light intensity value of the second reference cell image and the target light intensity value is greater than a second preset deviation threshold; If the second light intensity deviation value is greater than the second preset deviation threshold, updating the second correlation function based on the target second shooting parameter and the actual light intensity value, substituting the target light intensity value into the updated second correlation function, and determining a new target second shooting parameter corresponding to the target light intensity; If the second light intensity deviation value is less than or equal to the second preset deviation threshold, the second detection scheme is terminated.

6. The cell image shooting method according to claim 4, characterized in that: The second shooting parameter is a magnification corresponding to the exposure gain, and the second correlation function between the second shooting parameter and the light intensity value is constructed according to the second cell image, including: A second coordinate system of second shooting parameters and light intensity values ​​is constructed, and linear fitting is performed on the at least two second cell images in the second coordinate system to obtain the second correlation function.

7. The cell image shooting method according to claim 4, characterized in that: The first shooting parameter is exposure time, and the acquiring of at least two first cell images includes: At least two first shooting parameters that are less than a second preset parameter threshold are selected, and the at least two first cell images are obtained by shooting based on the at least two first shooting parameters, each of the first cell images corresponds to a unique first shooting parameter, and the second preset parameter threshold is less than the first preset parameter threshold.

8. A cell image taking device, characterized in that: include: A sample acquisition module, wherein the sample acquisition module is used to acquire at least two first cell images, wherein the at least two first cell images are cell images taken under different first shooting parameters, and the at least two first cell images have different light intensity values; A function construction module, wherein the function construction module is used to construct a first correlation function between a first shooting parameter and a light intensity value according to the first cell image; A parameter determination module, the parameter determination module is used to substitute the target light intensity value into the first correlation function to determine a target first shooting parameter corresponding to the target light intensity; A shooting module is used to shoot a first reference cell picture based on the target first shooting parameter, and use the first reference cell picture as the target cell picture corresponding to the target light intensity value.

9. An electronic device, characterized in that: include: at least one processor; and, a memory communicatively coupled to the at least one processor; Wherein, the memory stores instructions that can be executed by the at least one processor, and the instructions are executed by the at least one processor so that the at least one processor can execute the cell image taking method as described in any one of claims 1 to 7.

10. A computer-readable storage medium storing a computer program, characterized in that: The computer program is executed by a processor to implement the cell image taking method according to any one of claims 1 to 7.

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