Nano wall-broken and traditional Chinese medicine fermented powder capable of improving prevention rate and cure rate of recessive mastitis of dairy cow and preparation method of nano wall-broken and traditional Chinese medicine fermented powder

Abstract

The invention relates to nano wall-broken and traditional Chinese medicine fermented powder for improving the prevention rate and the cure rate of recessive mastitis of dairy cows and a preparation method of the nano wall-broken and traditional Chinese medicine fermented powder. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 2-4 parts of astragalus membranaceus, 2-4 parts of angelica sinensis, 1-3 parts of spina gleditsiae, 2-4 parts of lithospermum stems and leaves, 1-3 parts of selfheal, 1-3 parts of madder stems and leaves, 1-3 parts of loofah sponge, 1-3 parts of malt and 1-3 parts of wheat awn. The invention also relates to a preparation method of the nano wall-broken and traditional Chinese medicine fermented powder for improving the prevention rate and the cure rate of the recessive mastitis of the dairy cow. The preparation method comprises the following steps: respectively treating the traditional Chinese medicines in the formula according to a nano wall-broken and fermentation technology, mixing, crushing and mixing again to obtain the nano wall-broken and traditional Chinese medicine fermented powder. The traditional Chinese medicine powder aims at improving the cure rate of the recessive mastitis of the dairy cow, the nanometer wall breaking and fermentation technologies are adopted in the preparation process according to the drug performance and materials, the drug dissolution availability is improved, the palatability is changed, drug administration is convenient, stress is small, and the prevention morbidity of the recessive mastitis and the cure rate after morbidity are high.

Description

Technical Field

[0001] The invention belongs to the field of traditional Chinese veterinary medicine, and specifically relates to a nano-wall-breaking + traditional Chinese medicine fermentation powder for improving the prevention rate and cure rate of latent mastitis in dairy cows and a preparation method thereof. The traditional Chinese medicine powder has the function of improving the prevention rate and cure rate of latent mastitis, and is especially used for the daily prevention and treatment of latent mastitis in dairy cows. Background Art

[0002] Cow mastitis is a common multiple disease in dairy cows, which is mainly manifested as inflammation of the udder substance and interstitium. Its symptoms include udder redness, swelling, pain, fever, etc. In the current large-scale dairy cow farming, mastitis is the disease that causes the most serious economic losses to the dairy industry, bringing serious harm and losses to dairy cow production.

[0003] Cow mastitis is clinically divided into clinical mastitis and latent mastitis. Clinical mastitis is a mastitis that can be easily found clinically; latent mastitis is a mastitis that does not show clinical symptoms. In particular, latent mastitis is not easy to detect because it has no obvious clinical symptoms. However, the loss of latent mastitis is particularly huge, and the incidence rate is more than ten times higher than that of clinical mastitis. It can also cause a decrease in milk production, with an annual decrease of about 10-40%. Hidden mastitis will reduce the quality, grade and price of milk, and milk fat, lactose and milk protein will all decrease, causing an increase in the detection rate of alcohol-positive milk. In addition, under certain conditions, latent mastitis will also turn into clinical mastitis.

[0004] The causes of mastitis in dairy cows mainly include invasion of pathogens, trauma, poor milking techniques, poor environmental hygiene, improper feeding and management, hormone imbalance, udder defects and other disease-induced factors. In addition, the incidence of mastitis will also increase in hot and rainy seasons due to high temperatures and the proliferation of pathogens.

[0005] The principle of treatment for latent mastitis is to regulate the body's immunity and treat it with antibiotics. However, the use of antibiotics not only easily leads to antibiotic residues and pathogen resistance in milk, which endangers the health of dairy cows, but also affects people's pursuit of green food. Traditional Chinese medicine and its planting by-products not only have multiple pharmacological effects such as anti-inflammatory, antibacterial and immunity-enhancing, but also can enhance the body's positive energy and prevent the further occurrence and development of inflammation. It can make up for the shortcomings of antibiotics that easily cause pathogen resistance and antibiotic residues, thus attracting widespread attention and making breakthrough research progress. However, due to the rough conventional preparation process of traditional Chinese medicine powder, the drug absorption and utilization rate is not high and the palatability is poor. The high production cost, cumbersome use, and inconvenient administration of traditional Chinese medicine injections and traditional Chinese medicine injections limit their application in veterinary clinics. Summary of the invention

[0006] In summary, the incidence of latent mastitis in dairy cows is high, and antibiotic treatment leads to antibiotic residues and pathogen resistance in milk, which endangers the health of dairy cows and causes potential harm to food safety. However, the preparation process of Chinese medicine powder preparations for treating mastitis is rough, inconvenient to use, and the production cost of Chinese medicine injection and Chinese medicine injection is high, the use is cumbersome, and the administration is inconvenient, which limits clinical use. Based on the above reasons, the present invention overcomes the traditional Chinese medicine powder technology, newly groups the prescription, prepares a Chinese medicine powder with good palatability, low stress, and is convenient for large-scale production and use, and improves the prevention rate and cure rate of latent mastitis in dairy cows.

[0007] The present invention reduces labor costs, reduces labor intensity, reduces decoction energy consumption, and improves the enthusiasm of veterinary work. It provides a Chinese medicine powder for improving the prevention rate and cure rate of latent mastitis in dairy cows and a preparation method thereof. It uses processing technologies such as nano-wall breaking and fermentation technology to solve the problems of difficult dissolution of effective ingredients and large dosage, and makes maximum use of Chinese medicinal materials such as lithospermum officinale, sapodilla thorn, malt, astragalus, and madder.

[0008] To solve the above problems, the present invention is implemented through the following technical solutions:

[0009] A Chinese medicinal powder for improving the prevention rate and cure rate of latent mastitis in dairy cows and a preparation method thereof are designed. The powder is made from the following raw materials in parts by weight: 2-4 parts of astragalus, 2-4 parts of angelica, 1-3 parts of sapodilla thorns, 2-4 parts of lithospermum officinale stems and leaves, 1-3 parts of whole herb of selfheal, 1-3 parts of madder stems and leaves, 1-3 parts of loofah, 1-3 parts of malt, and 1-3 parts of wheat awn.

[0010] Preferably, the Chinese herbal powder for improving the prevention rate and cure rate of latent mastitis in dairy cows comprises: 4 parts of astragalus, 3 parts of angelica, 1 part of sapodilla thorn, 4 parts of lithospermum officinale stems and leaves, 1 part of whole herb of selfheal, 1 part of madder stems and leaves, 1 part of loofah, 2 parts of malt, and 3 parts of wheat awn.

[0011] A method for preparing the above-mentioned Chinese medicine powder for improving the prevention rate and cure rate of latent mastitis in dairy cows is designed, comprising the following steps:

[0012] (1) 4 parts of Astragalus, 3 parts of Angelica, 1 part of Gleditsia sinensis, 4 parts of Lithospermum officinale stems and leaves, 1 part of Prunella vulgaris whole plant, 1 part of Rubia cordifolia stems and leaves, and 2 parts of malt were subjected to nano-wall breaking using a steam explosion device specifically for traditional Chinese medicine.

[0013] (2) 1 part of loofah sponge and 3 parts of wheat awn are mixed and coarsely crushed into 80 mesh, and cellulase and Bacillus subtilis (mass ratio 1:1) are added in a ratio of 200:1, and then placed in a fermentation bag for solid-state fermentation at room temperature.

[0014] (3) The broken wall drug obtained in step (1) and the fermented drug obtained in step (2) are uniformly mixed and crushed to obtain a nano-broken wall + Chinese medicine fermented powder for improving the prevention rate and cure rate of latent mastitis in dairy cows.

[0015] The dosage of the present invention for treating latent mastitis is: 200g / head / time mixed with feed, once a day, for 3 to 5 consecutive days. The preventive dosage is 100g / head / time mixed with feed, and continuously fed for 3 days every month.

[0016] The positive and beneficial effects of the present invention compared to the prior art are:

[0017] (1) The present invention combines nano-wall breaking technology with fermentation extraction technology to prepare a Chinese medicine compound preparation for preventing and treating latent mastitis in dairy cows, which changes the palatability, facilitates the absorption and utilization of effective ingredients, effectively reduces labor costs, reduces labor intensity, improves the enthusiasm of veterinary work, and greatly improves the healing effect.

[0018] (2) The present invention uses nano-wall-breaking technology to ultrafinely grind easily crushed Chinese medicinal materials such as astragalus, angelica, Gleditsia sinensis, lithospermum officinale stems and leaves, whole herb of selfheal, madder stems and leaves, and malt, thereby fully improving the absorption and utilization rate of each Chinese medicinal material.

[0019] (3) The present invention uses fermentation technology to make drugs with more fiber, such as loofah and wheat awn, to change the palatability of the drugs and improve the utilization rate of the drugs.

[0020] (4) The present invention determines the composition and proportion of the prescription through a large number of experiments and screening, namely: 4 parts of Astragalus, 3 parts of Angelica, 1 part of Gleditsia sinensis, 4 parts of Lithospermum officinale stems and leaves, 1 part of Prunella vulgaris whole plant, 1 part of Rubia cordifolia stems and leaves, 1 part of Luffa, 2 parts of Malt, and 3 parts of Wheat awn. This is also not recorded in the prior art.

[0021] (5) The present invention overcomes the traditional Chinese medicine powder technology, newly formulates the prescription, and adopts nano-wall breaking technology and fermentation technology to produce a product that is easy to feed, has good palatability, is stable, and is convenient for large-scale production and use.

[0022] The invention reduces the energy consumption of decocting, improves the palatability and digestibility of traditional Chinese medicine, provides a traditional Chinese medicine powder for improving the prevention rate and cure rate of latent mastitis in dairy cows and a preparation method thereof, and uses nano-wall-breaking technology and fermentation technology to process the medicinal materials according to their properties, so that various traditional Chinese medicinal materials can be utilized to the maximum extent, and solves the problem of reusing non-medicinal parts when harvesting ginger.

[0023] In the formula, Astragalus and Angelica are the main drugs. Astragalus replenishes qi and has anti-stress and extensive antibacterial effects. Angelica replenishes blood and regulates blood, regulates menstruation and relieves pain, moistens the intestines and promotes bowel movements, and promotes blood circulation and removes blood stasis. Astragalus and Angelica are used together to replenish qi and blood, reduce stress and enhance immunity, and enhance the body's positive energy of dairy cows. The stems and leaves of Lithospermum officinale and the stems and leaves of Rubia cordifolia are both non-medicinal parts of medicinal plants, and both have the effects of clearing heat, detoxifying, cooling blood, promoting blood circulation and removing blood stasis. They are all ministerial drugs for treating blockage caused by breast inflammation. Gleditsia sinensis, whole herb of Prunella vulgaris, loofah, and wheat awn are all adjuvants. Gleditsia sinensis is mainly used to treat carbuncle, swelling and postpartum milk deficiency. Loofah has the effects of promoting blood circulation and activating collaterals, clearing heat and detoxifying. The whole herb of Prunella vulgaris is the dried fruit spike (medicinal part) of Prunella vulgaris plus dried stems and leaves, which have the effects of clearing heat and purging fire, improving eyesight, dispersing nodules and reducing swelling. Prunella vulgaris is a "holy medicine" for clearing and protecting the liver, and the liver meridian runs through the breast. Wheat awns can invigorate blood circulation and menstruation, clear away heat and promote diuresis. Gleditsia sinensis, Prunella vulgaris, loofah and wheat awns all have the effect of promoting menstruation and lactation. The above-mentioned drugs can be used together to replenish qi and blood, reduce swelling and expel carbuncle, and have significant preventive and therapeutic effects on the treatment of latent mastitis. DETAILED DESCRIPTION

[0024] The following is a detailed description of the specific implementation methods provided by the present invention in conjunction with the embodiments:

[0025] Example 1

[0026] A method for preparing the above-mentioned Chinese medicine powder for improving the prevention rate and cure rate of latent mastitis in dairy cows is designed, comprising the following steps (taking cows as an example):

[0027] (1) 4 kg of Astragalus, 3 kg of Angelica, 1 kg of Gleditsia sinensis, 1 kg of Prunella vulgaris, and 2 kg of malt were nano-broken using a steam explosion device specifically designed for traditional Chinese medicine.

[0028] (2) 1 kg of loofah sponge and 3 kg of wheat awn were mixed and coarsely crushed to 80 mesh, and solid-state fermentation was performed at room temperature using cellulase and Bacillus subtilis (mass ratio 1:1) in a mass ratio of 200:1;

[0029] (3) The broken wall drug of step (1) and the fermented drug obtained in step (2) are uniformly mixed, and 4 kg of stems and leaves of Lithospermum erythrorhizon and 1 kg of stems and leaves of Rubia cordifolia are added and crushed to obtain a nano-broken wall + Chinese medicine fermented powder for improving the prevention rate and cure rate of latent mastitis in dairy cows.

[0030] Example 2

[0031] A method for preparing the above-mentioned Chinese medicine powder for improving the prevention rate and cure rate of latent mastitis in dairy cows is designed, comprising the following steps:

[0032] (1) 40 kg of Astragalus, 30 kg of Angelica, 10 kg of Gleditsia sinensis, 10 kg of Prunella vulgaris whole herb, and 20 kg of malt were nano-broken using a steam explosion device specifically designed for traditional Chinese medicine.

[0033] (2) 10 kg of loofah and 30 kg of wheat awn were mixed and coarsely crushed to 80 mesh, and solid-state fermentation was performed at room temperature using cellulase and Bacillus subtilis (mass ratio 1:1) in a mass ratio of 200:1;

[0034] (3) The broken wall drug of step (1) and the fermented drug obtained in step (2) are uniformly mixed, and 40 kg of stems and leaves of Lithospermum erythrorhizon and 10 kg of stems and leaves of Rubia cordifolia are added and crushed to obtain a nano-broken wall + Chinese medicine fermented powder for improving the prevention rate and cure rate of latent mastitis in dairy cows.

[0035] Example 3 Prescription Screening Test

[0036] 1. Purpose

[0037] Screening of traditional Chinese medicine prescriptions for preventing and treating subclinical mastitis.

[0038] 2. Prescription used (each medicine contains 1 portion)

[0039] Recipe 1: Astragalus and Angelica

[0040] Recipe 2: Astragalus, Chinese Angelica, Dandelion, Gleditsia sinensis, Vaccaria segetalis, Pulsatilla scabra

[0041] Recipe 3: Astragalus, Chinese Angelica, Dandelion, Honeysuckle, Prunella Vulgaris, Vaccaria, Malt

[0042] Recipe 4: Astragalus, Chinese Angelica, Gleditsia sinensis, Prunella vulgaris, malt, loofah, wheat awn

[0043] Recipe 5: Astragalus, Chinese angelica, Gleditsia sinensis thorn, whole herb of Prunella vulgaris, malt, loofah, wheat awn, stems and leaves of Lithospermum officinale, and stems and leaves of Rubia cordifolia.

[0044] 3. Object

[0045] A total of 50 cattle in a ranch in Henan tested positive for CMT.

[0046] 4. Methods and steps

[0047] The participating cattle were divided into 5 groups. Groups 1 to 5 were given 150 g of the above-mentioned recipe 1, recipe 2, recipe 3, recipe 4, and recipe 5, respectively, for 4 consecutive days, and the cure rate and effectiveness rate of mastitis were calculated.

[0048] 5. Results

[0049] As can be seen from the results in Table 1, the cure rate and the effective rate of Recipe 5 are both the highest, indicating that the comprehensive combination of tonic drugs, heat-clearing drugs, and menstrual-promoting and lactation-promoting drugs in Recipe 5 has a more definite therapeutic effect on latent mastitis in dairy cows. Therefore, Recipe 5 of this embodiment is determined as a backup prescription for later research.

[0050] Table 1 Prescription screening test results

[0051]

[0052] Example 4 Prescription ratio determination test

[0053] 1. Purpose

[0054] Determine the composition ratio of traditional Chinese medicine prescriptions for preventing and treating latent mastitis in dairy cows.

[0055] 2. Prescription used

[0056] Prescription 1: 1 part of Astragalus, 1 part of Angelica, 1 part of Gleditsia sinensis, 1 part of Prunella Vulgaris, 1 part of Malt, 1 part of Loofah, 1 part of Wheat Awning, 1 part of Lithospermum officinale Stem and Leaf, 1 part of Rubia cordifolia Stem and Leaf;

[0057] Prescription 2: 2 parts of Astragalus, 2 parts of Angelica, 3 parts of Gleditsia sinensis, 2 parts of Prunella Vulgaris, 2 parts of Malt, 1 part of Loofah, 1 part of Wheat Awning, 1 part of Lithospermum officinale stem and leaf, 1 part of Rubia cordifolia stem and leaf;

[0058] Prescription 3: 4 parts of Astragalus, 3 parts of Angelica, 1 part of Gleditsia sinensis, 1 part of Prunella vulgaris, 2 parts of malt, 1 part of loofah, 3 parts of wheat awn, 4 parts of Lithospermum officinale stems and leaves, 1 part of Rubia cordifolia stems and leaves;

[0059] Prescription 4: 4 parts of Astragalus, 3 parts of Angelica, 1 part of Gleditsia sinensis, 1 part of Prunella vulgaris, 1 part of malt, 2 parts of loofah, 1 part of wheat awn, 1 part of Lithospermum officinale stem and leaf, 2 parts of Rubia cordifolia stem and leaf;

[0060] 3. Object

[0061] A total of 40 cattle tested positive for CMT at a ranch in Inner Mongolia.

[0062] 4. Methods and steps

[0063] All adult cows participating in the experiment were divided into 4 groups, and 150 g of the above prescriptions 1, 2, 3, 4, and 5 were mixed with milk and given to the cows respectively for 4 consecutive days, and the cure rate and effectiveness rate of mastitis were calculated.

[0064] 5. Results and Analysis

[0065] It can be seen from the results in Table 2 that the cure rate and effective rate of mastitis in Formulation 3 are both the highest, the effective rate of Formulation 2 is higher than that of Formulation 4, and the cure rate of Formulation 4 is higher than that of Formulation 2. The reason may be that the components of the tonic medicines are different. Therefore, Formulation 3 of this embodiment is determined as the final formulation for preventing and treating latent mastitis.

[0066] Table 2 Results of the test for determining the proportion of the formula

[0067]

[0068] Example 5 Prescription Pharmacodynamics Test

[0069] Pharmacodynamic study on latent mastitis in dairy cows using the Chinese medicine powder prepared in Example 2

[0070] 1. Test methods

[0071] In a large dairy farm, 29 cows tested positive for latent mastitis by CMT. Three days later, CMT test confirmed latent mastitis and the cows were given medication, 100g per cow per day, once a day for three consecutive days. The milk production before and after the test and the efficacy and cure rate of mastitis treatment were recorded.

[0072] 2. Test results and analysis

[0073] Table 3 The therapeutic effect of Chinese herbal powder on latent mastitis

[0074]

[0075] Table 4 Changes in milk production of cows with latent mastitis before and after the experiment

[0076]

[0077] Through the analysis of experimental data: From the test results in Table 3, it can be seen that the cure rate of the test group after the test was 76.9%, and the effective rate (improvement + cure) was 96.6%. The Chinese medicine compound preparation for preventing and treating mastitis also has a good effect on the prevention and treatment of mastitis in dairy cows. Table 4 shows that the average milk production of dairy cows after treatment is 3.4kg higher than before the test, which is 9.8% higher than before the test. This shows that the Chinese medicine compound preparation for preventing and treating mastitis can significantly increase milk production while treating mastitis.

[0078] Chinese medicinal materials have a wide range of sources and not only have multiple pharmacological effects such as antibacterial and anti-inflammatory effects, but also have the characteristics of no residue, no toxic side effects, and less drug resistance. The by-products of Chinese medicinal planting and production (the whole herb, wheat awn, lithospermum stems and leaves, and madder stems and leaves in this patent) have huge output, extensive resources, and definite medicinal value. Therefore, using them for the prevention and treatment of mastitis in dairy cows can not only expand the scope of medicinal resources, save medicinal resources, but also reduce breeding costs, which is beneficial to the food safety of dairy products.

[0079] The above description is only a specific implementation mode of the present invention, but the protection scope of the present invention is not limited thereto. Any modifications, equivalent substitutions and improvements made by any technician familiar with the technical field within the technical scope disclosed by the present invention and within the spirit and principle of the present invention should be covered by the protection scope of the present invention.

Claims

1. A nano-wall-broken + Chinese medicine fermented powder for improving the prevention rate and cure rate of latent mastitis in dairy cows and a preparation method thereof, characterized in that: The invention is prepared from the following raw materials in parts by weight: ingredients: 2-4 parts of astragalus, 2-4 parts of angelica, 1-3 parts of sapodilla thorns, 2-4 parts of lithospermum officinale stems and leaves, 1-3 parts of whole herb of selfheal, 1-3 parts of madder stems and leaves, 1-3 parts of loofah, 1-3 parts of malt and 1-3 parts of wheat awn.

2. The Chinese medicinal compound preparation according to claim 1, characterized in that: The invention is prepared from the following raw materials in parts by weight: 4 parts of astragalus, 3 parts of angelica, 1 part of sapodilla thorn, 4 parts of lithospermum officinale stems and leaves, 1 part of selfheal whole plant, 1 part of madder stems and leaves, 1 part of loofah, 2 parts of malt and 3 parts of wheat awn.

3. The method for preparing the nano-wall-breaking + Chinese medicine fermentation powder for improving the prevention rate and cure rate of latent mastitis in dairy cows according to claim 1, characterized in that: The following steps are involved: (1) 4 parts of astragalus, 3 parts of angelica, 1 part of Gleditsia sinensis, 4 parts of lithospermum officinale stems and leaves, 1 part of Prunella vulgaris whole plant, 1 part of madder stems and leaves, and 2 parts of malt were prepared by nano-wall-breaking technology; (2) 1 part of loofah and 3 parts of wheat awn are fermented; (3) The broken wall drug obtained in step (1) and the fermented drug obtained in step (2) are uniformly mixed and crushed to obtain a nano-broken wall + Chinese medicine fermented powder for improving the prevention rate and cure rate of latent mastitis in dairy cows.

4. According to claim 3, the preparation step (1) uses nano-wall breaking using a steam explosion device specifically designed for traditional Chinese medicine.

5. According to claim 3, the fermentation process described in the preparation step (2) adopts solid fermentation technology.

6. According to claim 3, the solid fermentation technology described in the preparation step (2) adopts the co-fermentation of cellulase and Bacillus subtilis.

7. The nano-wall-broken + Chinese medicine fermented powder for improving the prevention rate and cure rate of latent mastitis in dairy cows according to claim 1, characterized in that: The Chinese medicine powder prepared by nano-wall breaking and fermentation technology has good palatability and a high cure rate for latent mastitis.

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