Osmanthus essential oil, extraction method and application thereof

Abstract

The present invention provides a kind of osmanthus essential oil and its extracting method and application, the extracting method of osmanthus essential oil, specifically comprises: S1, pretreatment: fresh, impurity-free osmanthus is subjected to repeated freeze-thaw 2 4 times, vacuum drying, obtain pre-treated osmanthus;S2, micronization: the pre-treated osmanthus of S1 is placed in ultra-micro airflow pulverizer, obtain osmanthus ultra-fine powder;S3, the osmanthus ultra-fine powder of S2 is added in ethanol-isopropanol-ammonium sulfate three-phase extraction system, ultrasonic treatment for the first time, adds cosolvent, ultrasonic treatment for the second time, collects extract, evaporates and removes cosolvent, and is concentrated under reduced pressure to obtain osmanthus essential oil. The osmanthus essential oil purity obtained by extraction is higher, and yield is high, and the osmanthus essential oil extracted by the present invention is used as inflammation inhibitor, has good TNF-α inhibition rate, has practical application value.

Description

Technical Field

[0001] The present invention relates to the field of essential oil extraction, in particular to osmanthus essential oil and an extraction method and application thereof. Background Art

[0002] Osmanthus fragrans, an evergreen tree or shrub with small, four-lobed corollas, has a distinctive sweet and fragrant aroma and flavor. Its use as an additive in food processing is extensive and has a long history, making it considered a traditional flower. Rich in polysaccharides, polyphenols, flavonoids, terpenes, and pigments, osmanthus fragrans and its products have antioxidant, anti-aging, anti-tumor, lipid-lowering, blood pressure-lowering, cough-relieving, phlegm-reducing, halitosis-relieving, and antibacterial properties, earning it the title "the king of all medicines."

[0003] Essential oils, also known as aromatic oils or volatile oils, are obtained from the flowers, stems, leaves, roots and fruits of plants through certain extraction techniques. Essential oils have a complex chemical composition, mainly including hydrocarbons (such as terpenes and sesquiterpenes) and oxygenated hydrocarbons (such as phenols, epoxides, ethers, esters, acids, alcohols and ketones). Essential oils have unique physical and chemical properties, including antibacterial, anti-inflammatory, antiviral, analgesic, anti-tumor, insect repellent, penetration-promoting, and antioxidant properties. Therefore, essential oils have been widely used in cosmetics, perfumes, fragrances, food and pharmaceutical industries. Extraction technology plays an important role in the research of essential oils. Different extraction techniques have a significant impact on the quality and yield of essential oils.

[0004] At present, the extraction technology of osmanthus essential oil includes steam distillation, supercritical extraction and organic solvent extraction, etc., and the osmanthus raw materials used for extraction are fresh osmanthus flowers, salted osmanthus flowers and frozen osmanthus flowers. However, steam distillation has a high operating temperature and large energy consumption, which easily causes the hydrolysis of some effective ingredients in the essential oil and the decomposition of heat-sensitive compounds. The extraction rate is low, and the extracted osmanthus essential oil carries water and is prone to mildew, which can cause the loss of active ingredients in the osmanthus essential oil and reduce its application value. Supercritical extraction is expensive and not suitable for large-scale production. Organic solvent extraction can cause the quality of osmanthus essential oil to deteriorate and its purity to decrease. Therefore, how to improve the quality and yield of essential oil and reduce the cost of the extraction process have become the problems faced by the field of osmanthus essential oil extraction technology. Summary of the Invention

[0005] In view of this, the present invention provides an osmanthus essential oil and an extraction method and application thereof.

[0006] The technical solution of the present invention is achieved as follows:

[0007] A method for extracting osmanthus essential oil, comprising the following steps:

[0008] S1. Pretreatment: fresh, impurity-free osmanthus flowers are repeatedly frozen and thawed 2-4 times, and vacuum-dried to obtain pretreated osmanthus flowers;

[0009] S2, micronization: placing the pretreated osmanthus flowers prepared in S1 in an ultrafine airflow mill to obtain ultrafine osmanthus powder;

[0010] S3. Add the osmanthus ultrafine powder of S2 to an ethanol-isopropanol-ammonium sulfate tri-phase extraction system, perform a first ultrasonic treatment, add a cosolvent, perform a second ultrasonic treatment, collect the extract, evaporate to remove the cosolvent, and concentrate under reduced pressure to obtain osmanthus essential oil.

[0011] Furthermore, in step S1, the freeze-thaw step is to first freeze at -5 to -2°C for 12 to 24 hours, and then thaw naturally at room temperature.

[0012] Furthermore, in step S2, the particle size of the osmanthus ultrafine powder is 1-50 μm.

[0013] Furthermore, in step S3, the solid-liquid ratio of the osmanthus ultrafine powder to the ethanol-isopropanol-ammonium sulfate triaqueous extraction system is 1:10-15 g / mL; the concentration of ammonium sulfate in the ethanol-isopropanol-ammonium sulfate triaqueous extraction system is 5%-10% w / v, the concentration of ethanol is 50%-70% v / v, and the concentration of isopropanol is 30%-50% v / v.

[0014] Furthermore, in step S3, the first ultrasonic treatment is performed at 20-30 kHz and 40-50° C. for 1-3 h; and the second ultrasonic treatment is performed at 20-30 kHz and 25-35° C. for 30-60 min.

[0015] Furthermore, in step S3, the co-solvent is lactic acid and glycerol in a mass ratio of 1:2-4; the volume ratio of the co-solvent to the ethanol-isopropanol-ammonium sulfate tri-aqueous extraction system is 0.01-0.03:1.

[0016] The invention discloses an osmanthus essential oil, which is extracted by the extraction method.

[0017] The osmanthus essential oil extracted by the extraction method is used in preparing products of inflammation inhibitors.

[0018] Furthermore, the concentration of osmanthus essential oil in the product is 1-5wt%.

[0019] Furthermore, the product is a skin care product, including lotion, lotion, cream, essence, ointment, and facial mask.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] 1. The extraction process of the present invention avoids the problems of sticking together into layers and insufficient and uneven cooking caused by directly steaming and extracting fresh osmanthus flowers. The extracted osmanthus essential oil has higher purity and high yield, and has practical application value. The osmanthus essential oil extracted by the present invention is used as an inflammation inhibitor and has a good TNF-α inhibition rate.

[0022] 2. The present invention repeatedly freezes and thaws fresh and impure osmanthus to loosen the plant cell structure, form a finer powder, and increase the release of effective ingredients. After ultrafine grinding of the osmanthus, the powder particle size and structure can be effectively improved. The surface area and porosity of the 1-50 μm ultrafine powder are greatly increased, the solubility is improved, and the subsequent extraction efficiency is improved. The ultrafine powder is subjected to ultrasonic extraction by a three-phase extraction system to fully and comprehensively extract the active components. A cosolvent of a specific composition is added to fully dissolve the osmanthus essential oil in the cosolvent, the effective ingredients in the essential oil are not hydrolyzed, the essential oil yield is higher, and the cosolvent is removed by evaporation to increase the purity of the osmanthus essential oil. DETAILED DESCRIPTION

[0023] In order to better understand the technical content of the present invention, specific examples are provided below to further illustrate the present invention.

[0024] Unless otherwise specified, the experimental methods used in the examples of the present invention are all conventional methods.

[0025] Unless otherwise specified, the materials, reagents, etc. used in the examples of the present invention can be obtained from commercial sources.

[0026] Example 1

[0027] A method for extracting osmanthus essential oil, comprising the following steps:

[0028] S1. Pretreatment: freeze-thaw fresh, impurity-free osmanthus flowers, first at -3°C for 18 h, then thaw naturally at room temperature, repeat freeze-thaw three times, and vacuum dry to obtain pretreated osmanthus flowers;

[0029] S2, micronization: placing the pretreated osmanthus fragrans prepared in S1 in an ultrafine airflow mill to obtain ultrafine osmanthus fragrans powder with a particle size of 20±1 μm;

[0030] S3. According to the solid-liquid ratio of 1:13 g / mL, the osmanthus ultrafine powder of S2 was added to the ethanol-isopropanol-ammonium sulfate triaqueous extraction system (the concentration of ammonium sulfate was 0.08 g / mL, the concentration of ethanol was 0.474 g / mL, and the concentration of isopropanol was 0.314 g / mL), and the first ultrasonic (25 kHz, 45 ° C) extraction was performed for 2 hours, and a cosolvent (lactic acid and glycerol in a mass ratio of 1:3) was added, and the volume ratio of the cosolvent to the ethanol-isopropanol-ammonium sulfate triaqueous extraction system was 0.02:1. Then, a second ultrasonic (25 kHz, 30 ° C) extraction was performed for 45 minutes, the extract was collected, the cosolvent was evaporated to remove the cosolvent, and the extract was concentrated under reduced pressure to obtain osmanthus essential oil.

[0031] Example 2

[0032] A method for extracting osmanthus essential oil, comprising the following steps:

[0033] S1. Pretreatment: freeze-thaw fresh, impurity-free osmanthus flowers, first at -5°C for 12 h, then thaw naturally at room temperature, repeat freeze-thaw twice, and vacuum dry to obtain pretreated osmanthus flowers;

[0034] S2, micronization: placing the pretreated osmanthus fragrans prepared in S1 in an ultrafine airflow mill to obtain ultrafine osmanthus fragrans powder with a particle size of 5±1 μm;

[0035] S3. According to the solid-liquid ratio of 1:10 g / mL, the osmanthus ultrafine powder of S2 was added to the ethanol-isopropanol-ammonium sulfate triaqueous extraction system (the concentration of ammonium sulfate was 0.05 g / mL, the concentration of ethanol was 0.3945 g / mL, and the concentration of isopropanol was 0.2355 g / mL), and the first ultrasonic (20 kHz, 40 ° C) extraction was performed for 1 hour, and a cosolvent (lactic acid and glycerol in a mass ratio of 1:2) was added, and the volume ratio of the cosolvent to the ethanol-isopropanol-ammonium sulfate triaqueous extraction system was 0.01:1. Then, a second ultrasonic (20 kHz, 25 ° C) extraction was performed for 30 minutes, the extract was collected, the cosolvent was evaporated to remove the cosolvent, and the extract was concentrated under reduced pressure to obtain osmanthus essential oil.

[0036] Example 3

[0037] A method for extracting osmanthus essential oil, comprising the following steps:

[0038] S1. Pretreatment: freeze-thaw fresh, impurity-free osmanthus flowers, first at -2°C for 24 hours, then thaw naturally at room temperature, repeat freeze-thaw 4 times, and vacuum dry to obtain pretreated osmanthus flowers;

[0039] S2, micronization: placing the pretreated osmanthus fragrans prepared in S1 in an ultrafine airflow mill to obtain ultrafine osmanthus fragrans powder with a particle size of 45±1 μm;

[0040] S3. According to the solid-liquid ratio of 1:15 g / mL, the osmanthus ultrafine powder of S2 was added to the ethanol-isopropanol-ammonium sulfate triaqueous extraction system (the concentration of ammonium sulfate was 0.1 g / mL, the concentration of ethanol was 0.5523 g / mL, and the concentration of isopropanol was 0.3925 g / mL), and the first ultrasonic (30 kHz, 50 ° C) extraction was performed for 3 hours, and a cosolvent (lactic acid and glycerol in a mass ratio of 1:4) was added, and the volume ratio of the cosolvent to the ethanol-isopropanol-ammonium sulfate triaqueous extraction system was 0.03:1. Then, a second ultrasonic (30 kHz, 35 ° C) extraction was performed for 60 minutes, the extract was collected, the cosolvent was evaporated to remove the cosolvent, and the extract was concentrated under reduced pressure to obtain osmanthus essential oil.

[0041] Comparative Example 1

[0042] The difference from Example 1 is that no pretreatment is performed, and the other aspects are the same as Example 1.

[0043] That is, the extraction method of the osmanthus essential oil of this comparative example, specifically comprising the following steps:

[0044] S1, micronization: fresh, pure osmanthus flowers are dried and placed in an ultrafine airflow mill to obtain osmanthus flower ultrafine powder with a particle size of 20±1 μm;

[0045] S2. According to the solid-liquid ratio of 1:13 g / mL, the osmanthus ultrafine powder was added to the ethanol-isopropanol-ammonium sulfate triaqueous extraction system (the concentration of ammonium sulfate was 0.08 g / mL, the concentration of ethanol was 0.474 g / mL, and the concentration of isopropanol was 0.314 g / mL), and the first ultrasonic extraction (25 kHz, 45 ° C) was performed for 2 h. A cosolvent (lactic acid and glycerol in a mass ratio of 1:3) was added, and the volume ratio of the cosolvent to the ethanol-isopropanol-ammonium sulfate triaqueous extraction system was 0.02:1. Then, a second ultrasonic extraction (25 kHz, 30 ° C) was performed for 45 min. The extract was collected, the cosolvent was evaporated to remove the cosolvent, and the extract was concentrated under reduced pressure to obtain osmanthus essential oil.

[0046] Comparative Example 2

[0047] The difference from Example 1 is that the powder is not micronized, and the other aspects are the same as Example 1.

[0048] That is, the extraction method of the osmanthus essential oil of this comparative example, specifically comprising the following steps:

[0049] S1. Pretreatment: freeze-thaw fresh, impurity-free osmanthus flowers, first at -3°C for 18 h, then thaw naturally at room temperature, repeat freeze-thaw three times, and vacuum dry to obtain pretreated osmanthus flowers;

[0050] S2. According to the solid-liquid ratio of 1:13 g / mL, the pretreated osmanthus was added to an ethanol-isopropanol-ammonium sulfate triaqueous extraction system (the concentration of ammonium sulfate was 0.08 g / mL, the concentration of ethanol was 0.474 g / mL, and the concentration of isopropanol was 0.314 g / mL), and the first ultrasonic extraction (25 kHz, 45 ° C) was performed for 2 h. A cosolvent (lactic acid and glycerol in a mass ratio of 1:3) was added, and the volume ratio of the cosolvent to the ethanol-isopropanol-ammonium sulfate triaqueous extraction system was 0.02:1. Then, a second ultrasonic extraction (25 kHz, 30 ° C) was performed for 45 min. The extract was collected, the cosolvent was evaporated to remove the cosolvent, and the extract was concentrated under reduced pressure to obtain osmanthus essential oil.

[0051] Comparative Example 3

[0052] The difference from Example 1 is that the pretreated osmanthus flowers are crushed into ordinary powder with a particle size of 250 μm, and the rest is the same as Example 1.

[0053] That is, the extraction method of the osmanthus essential oil of this comparative example, specifically comprising the following steps:

[0054] S1. Pretreatment: freeze-thaw fresh, impurity-free osmanthus flowers, first at -3°C for 18 h, then thaw naturally at room temperature, repeat freeze-thaw three times, and vacuum dry to obtain pretreated osmanthus flowers;

[0055] S2, micronization: crush the pretreated osmanthus fragrans prepared in S1, sieve, and collect osmanthus fragrans powder with a particle size of 250 μm;

[0056] S3. According to the solid-liquid ratio of 1:13 g / mL, the osmanthus powder of S2 was added to the ethanol-isopropanol-ammonium sulfate triaqueous extraction system (the concentration of ammonium sulfate was 0.08 g / mL, the concentration of ethanol was 0.474 g / mL, and the concentration of isopropanol was 0.314 g / mL), and the first ultrasonic (25 kHz, 45 ° C) extraction was performed for 2 hours, and a cosolvent (lactic acid and glycerol in a mass ratio of 1:3) was added, and the volume ratio of the cosolvent to the ethanol-isopropanol-ammonium sulfate triaqueous extraction system was 0.02:1. Then, a second ultrasonic (25 kHz, 30 ° C) extraction was performed for 45 minutes, the extract was collected, the cosolvent was evaporated to remove the cosolvent, and the extract was concentrated under reduced pressure to obtain osmanthus essential oil.

[0057] Comparative Example 4

[0058] The difference from Example 1 is that the ethanol-isopropanol-ammonium sulfate tri-aqueous extraction system is replaced with an ethanol solution with a concentration of 60% v / v, and the rest is the same as Example 1.

[0059] That is, the extraction method of the osmanthus essential oil of this comparative example, specifically comprising the following steps:

[0060] S1. Pretreatment: freeze-thaw fresh, impurity-free osmanthus flowers, first at -3°C for 18 h, then thaw naturally at room temperature, repeat freeze-thaw three times, and vacuum dry to obtain pretreated osmanthus flowers;

[0061] S2, micronization: placing the pretreated osmanthus fragrans prepared in S1 in an ultrafine airflow mill to obtain ultrafine osmanthus fragrans powder with a particle size of 20±1 μm;

[0062] S3. According to the solid-liquid ratio of 1:13 g / mL, the osmanthus ultrafine powder of S2 was added to a 60% v / v ethanol solution, and the first ultrasonic (25 kHz, 45°C) extraction was performed for 2 hours. A cosolvent (lactic acid and glycerol in a mass ratio of 1:3) was added, and the volume ratio of the cosolvent to the 60% v / v ethanol solution was 0.02:1. Then, a second ultrasonic (25 kHz, 30°C) extraction was performed for 45 minutes. The extract was collected, the cosolvent was evaporated to remove the extract, and the extract was concentrated under reduced pressure to obtain osmanthus essential oil.

[0063] Comparative Example 5

[0064] The difference from Example 1 is that no co-solvent is added, and the other steps are the same as Example 1.

[0065] That is, the extraction method of the osmanthus essential oil of this comparative example, specifically comprising the following steps:

[0066] S1. Pretreatment: freeze-thaw fresh, impurity-free osmanthus flowers, first at -3°C for 18 h, then thaw naturally at room temperature, repeat freeze-thaw three times, and vacuum dry to obtain pretreated osmanthus flowers;

[0067] S2, micronization: placing the pretreated osmanthus fragrans prepared in S1 in an ultrafine airflow mill to obtain ultrafine osmanthus fragrans powder with a particle size of 20±1 μm;

[0068] S3. According to the solid-liquid ratio of 1:13 g / mL, the osmanthus ultrafine powder of S2 was added to the ethanol-isopropanol-ammonium sulfate triaqueous extraction system (the concentration of ammonium sulfate was 0.08 g / mL, the concentration of ethanol was 0.474 g / mL, and the concentration of isopropanol was 0.314 g / mL), and ultrasonic extraction (25 kHz, 45 ° C) was performed for 2 h. The extract was collected and concentrated under reduced pressure to obtain osmanthus essential oil.

[0069] 1. Performance test data

[0070] The performance of the osmanthus essential oils prepared by specific processes in Examples 1-3 and Comparative Examples 1-5 was evaluated, and the data results are shown in Table 1.

[0071] The formula for calculating the essential oil extraction rate is: essential oil extraction rate = (essential oil mass / fresh impurity-free osmanthus mass) × 100%

[0072] The contents of hexahydroxytaxene and dihydro-β-ionol were determined by GC-MS analysis.

[0073] Chromatographic conditions: The chromatographic column was HP-5MS, a quartz capillary column (30 m × 0.25 mm, 0.25 μm), and the carrier gas was high-purity nitrogen.

[0074] Injection method: injection volume is 0.5 μL, split ratio is 5:1, and injection port temperature is 250 °C.

[0075] Temperature program: initial temperature was 60 °C, increased to 280 °C at a rate of 4 °C / min, and maintained for 1 min.

[0076] Mass spectrometry conditions: electron impact (EI) electron source, ion source temperature 230°C, electron energy 70 eV, scanning mass range 30-550 amu.

[0077] Table 1

[0078]

[0079]

[0080] As can be seen from Table 1, compared with Comparative Examples 1-5, the repeated freeze-thaw pretreatment, ultrafine grinding, three-phase extraction system ultrasonic extraction and cosolvent are all indispensable processes of the present invention, which together improve the extraction rate of osmanthus essential oil.

[0081] 2. Inflammatory factor inhibition experiment

[0082] Study subjects: RAW 264.7 macrophages

[0083] The cellular inflammation model was established by stimulating cells with lipopolysaccharide (LPS) (bacterial endotoxin). The osmanthus essential oil (concentration 1%) of Example 1 and 0.5% quercetin were added as a positive control group. The cells were cultured for 24 h, and the cell supernatant was collected. The release level of the pro-inflammatory inflammatory factor TNF-α of RAW 264.7 macrophages was analyzed by ELISA kit. The results are shown in Table 2.

[0084] Table 2

[0085] sample TNF-α inhibition rate / % Example 1 57.8 Positive control group 55.7

[0086] As can be seen from Table 2, the osmanthus essential oil of Example 1 of the present invention has a good TNF-α inhibition rate, has a good anti-inflammatory effect, and can be used as an inflammation inhibitor.

[0087] 3. Irritation test

[0088] With reference to the Technical Specifications for Safety of Cosmetics (2015 edition), a closed patch test was performed behind the ear of the test subject. 0.02 mL of the test substance was dropped into the patch tester, which was then sealed behind the subject's left ear with tape. The test substance patch tester was removed after 30 minutes, and the skin reaction was observed and the score was recorded.

[0089] Among them, skin reaction is a negative reaction and the score is 0;

[0090] The skin reaction is a suspicious reaction with only slight erythema and a score of 1;

[0091] Skin reactions include erythema, infiltration, edema, and papules, with a score of 2;

[0092] Skin reactions included erythema, infiltration, edema, and herpes, with a score of 3;

[0093] The skin reactions included obvious erythema, severe infiltration, edema, and confluent blisters, with a score of 4.

[0094] The test results of each group were averaged, and the specific results are shown in Table 3.

[0095] Table 3

[0096] sample Rating level result Examples 1-3 0 No stimulation

[0097] As can be seen from Table 3, the osmanthus essential oils prepared in Examples 1-3 of the present invention are non-irritating and harmless to the skin and are highly safe.

[0098] Application Examples

[0099] The osmanthus essential oil essence comprises the following raw materials, calculated by mass percentage: 3% osmanthus essential oil, 10% 1,3-butylene glycol, 1% triethanolamine, 0.1% p-hydroxyacetophenone, 0.5% disodium edetate, and the balance water, totaling 100%.

[0100] The preparation method of osmanthus essential oil essence comprises the following specific steps:

[0101] (1) 1,3-Butanediol, triethanolamine, p-hydroxyacetophenone and disodium ethylenediaminetetraacetate are uniformly mixed to obtain a premix;

[0102] (2) adding the premix to water and treating with ultrasound (40 kHz, 50°C) for 40 min to obtain a mixed solution;

[0103] (3) Add osmanthus essential oil to the mixture, homogenize (temperature 65°C, first stir at 4000 rpm for 10 min, then continue stirring at 2000 rpm for 15 min), filter, and sterilize to obtain osmanthus essential oil essence.

[0104] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A method for extracting osmanthus essential oil, characterized in that: The specific steps include: S1. Pretreatment: fresh, impurity-free osmanthus flowers are repeatedly frozen and thawed 2-4 times, and vacuum-dried to obtain pretreated osmanthus flowers; S2, micronization: placing the pretreated osmanthus flowers prepared in S1 in an ultrafine airflow mill to obtain ultrafine osmanthus flower powder with a particle size of 1-50 μm; S3, adding the osmanthus ultrafine powder of S2 to an ethanol-isopropanol-ammonium sulfate three-phase extraction system, performing a first ultrasonic treatment, adding a cosolvent, performing a second ultrasonic treatment, collecting the extract, evaporating to remove the cosolvent, and concentrating under reduced pressure to obtain osmanthus essential oil; The concentration of ammonium sulfate in the ethanol-isopropanol-ammonium sulfate tri-aqueous extraction system is 5%-10% w / v, the concentration of ethanol is 50%-70% v / v, and the concentration of isopropanol is 30%-50% v / v; The first ultrasonic treatment is performed at 20-30 kHz and 40-50° C. for 1-3 h; the second ultrasonic treatment is performed at 20-30 kHz and 25-35° C. for 30-60 min; The cosolvent is lactic acid and glycerol in a mass ratio of 1:2-4; the volume ratio of the cosolvent to the ethanol-isopropanol-ammonium sulfate tri-phase extraction system is 0.01-0.03:

1.

2. The method for extracting osmanthus essential oil according to claim 1, wherein In step S1, the freeze-thaw step is to first freeze the sample at -5 to -2°C for 12 to 24 hours and then thaw the sample naturally at room temperature.

3. The method for extracting osmanthus essential oil according to claim 1, wherein In step S3, the solid-liquid ratio of the osmanthus ultrafine powder to the ethanol-isopropanol-ammonium sulfate tri-aqueous extraction system is 1:10-15 g / mL.

4. An osmanthus essential oil, characterized in that The extract is obtained by the extraction method according to any one of claims 1 to 3.

5. Use of the osmanthus essential oil obtained by the extraction method according to any one of claims 1 to 3 in the preparation of products that inhibit inflammation.

6. The use according to claim 5, characterized in that The concentration of osmanthus essential oil in the product is 1-5wt%.

7. The use according to claim 5, characterized in that The products are skin care products, including lotion, emulsion, cream, essence, ointment and facial mask.

Images