Animal heart perfusion device

Abstract

The invention discloses an animal heart perfusion device, and relates to the technical field of animal heart perfusion, the animal heart perfusion device comprises an experiment platform liquid supply unit, an anesthesia test tube, a limb fixing structure and a head fixing structure, the anesthesia test tube is installed on an experiment platform, and the head fixing structure comprises a V-shaped elastic rope used for tensioning the head of an animal; two ends of the elastic rope are respectively fixed on the experimental platform; a volatilization opening of the anesthesia test tube corresponds to the nose of an animal; after the four limbs of the animal are fixed, the head of the animal is tensioned through the elasticity of the elastic rope, the head of the animal is fixed at the moment, the nose of the animal can be always kept corresponding to a volatilization opening of an anesthesia test tube in cooperation with the anesthesia test tube installed on the experiment platform, the anesthesia effect is improved, meanwhile, the anesthesia test tube does not need to be always held during perfusion, and the operation is convenient. In addition, the fixation of the head of the animal is matched with the fixation of the four limbs, so that the stability of the animal in the experiment process is facilitated, and the experiment success rate is increased.

Description

Technical Field

[0001] The invention relates to the technical field of animal heart perfusion, in particular to an animal heart perfusion device. Background Art

[0002] Cardiac perfusion is an experimental technique that is mainly used before experiments such as brain tissue sectioning, immunostaining, or in situ hybridization. Taking mice as an example, the mice are first anesthetized, then fixed, and an infusion needle is inserted into the left ventricle. At this time, a right atrial opening is made and normal saline is injected to drain the blood from the mouse. When the liquid discharged from the right atrial appendage becomes clear, the blood is completely drained, and then it is necessary to switch to the input of paraformaldehyde, which is used to fully fix the various organs in the mouse. After fixation, the sample can be taken.

[0003] Due to the different sizes of animals, the required anesthetic doses are different. The animals may be awake during the perfusion. In order to maintain the anesthetic effect of the animals, it is necessary to provide them with continuous anesthesia during the perfusion. Inhalation anesthetics are a commonly used anesthesia method. The gaseous anesthetic evaporated from the liquid inhalation anesthetic is used for the breathing of the animals that have not yet died, thereby continuously maintaining the anesthetic effect. However, the anesthetic tube containing the liquid inhalation anesthetic needs to be manually held by a person. When operating alone, the person needs to hold the anesthetic tube and perfuse the animal, which makes the operation inconvenient.

[0004] Therefore, people are in urgent need of an animal heart perfusion device that is easy to operate and has good anesthesia effect. Summary of the invention

[0005] The purpose of the present invention is to provide an animal heart perfusion device to solve the problems existing in the above-mentioned prior art. An anesthetic test tube is installed on an experimental platform, and the animal's head is fixed with an elastic rope so that it corresponds to the volatilization port of the anesthetic test tube, thereby improving the anesthetic effect on the animal and improving the convenience of operation.

[0006] To achieve the above-mentioned purpose, the present invention provides the following scheme: The present invention provides an animal heart perfusion device, comprising an experimental platform, a liquid supply unit for supplying perfusion liquid to the animal heart, an anesthesia test tube containing liquid inhalation anesthetic, a limb fixing structure for fixing the animal's limbs, and a head fixing structure for fixing the animal's head, the anesthesia test tube is installed on the experimental platform, the head fixing structure includes a V-shaped elastic rope for tightening the animal's head, the two ends of the elastic rope are respectively fixed on the experimental platform, and the volatilization port of the anesthesia test tube is arranged corresponding to the nose of the animal.

[0007] Preferably, the experimental platform is provided with a clamping piece with elastic self-restoring ability, the clamping piece is provided with a clamping slot matching the anesthesia test tube, and the anesthesia test tube is clamped in the clamping slot.

[0008] Preferably, the liquid supply unit includes a peristaltic pump, a delivery hose, an infusion needle and a liquid tank, the liquid tank includes at least one liquid storing area, the liquid storing area is connected to the liquid inlet pipe of the peristaltic pump, and the liquid outlet pipe of the peristaltic pump is connected to the infusion needle through the delivery hose.

[0009] Preferably, when there are two or more liquid holding areas, the multiple liquid holding areas are respectively connected to one peristaltic pump, the multiple peristaltic pumps are connected to the infusion needles through a multi-way connector, and a flow regulator is provided on the pipeline connecting the multi-way connector and the peristaltic pump; or the multiple liquid holding areas are connected to the liquid inlet pipe of a single peristaltic pump through a multi-way connector, the peristaltic pump is connected to the infusion needle through the delivery hose, and a flow regulator is provided on the pipeline connecting the multi-way connector and the liquid holding areas.

[0010] Preferably, the experimental platform comprises a box cover and a box body, the box cover is buckled on the box body, and through holes are provided on the box cover at positions corresponding to both sides of the animal's chest cavity for the perfusion fluid and blood to flow into the box body.

[0011] Preferably, the limb fixing structure includes a fixing needle and a fixing hole, the box cover is provided with the fixing hole matching the fixing needle, and the fixing needle is inserted into the fixing hole after passing through the animal. Preferably, a plurality of the fixing holes are provided corresponding to the position of each limb of the animal, and the plurality of the fixing holes are arranged in sequence along the length direction of the animal.

[0012] Preferably, the fixing structure includes a clamping rod and a limiting structure, wherein the length direction of the clamping rod is parallel to the length direction of the animal's body, one end of the clamping rod is rotatably arranged above the experimental platform, and the other end is connected to the limiting structure for fixing the end of the clamping rod, and the end of the clamping rod close to the experimental platform is provided with a clamping groove for squeezing the forelimbs and hind limbs on one side of the animal onto the experimental platform, and a flexible pad for protecting the animal's limbs is provided in the clamping groove.

[0013] Preferably, the fixing structure also includes a rotating screw and a connecting piece, wherein the rotating screw is rotatably arranged on the connecting piece, and the connecting piece is connected to a side of the experimental platform away from the pin, and the rotating screw includes a first thread segment and a second thread segment with opposite threads, and the first thread segment and the second thread segment are respectively threadedly connected to the clamping rod.

[0014] Preferably, the experimental platform is provided with a fixing device for fixing the infusion needle of the liquid supply unit, and the fixing device includes a vertical plate and a positioning clamp. The vertical plate is vertically arranged on the experimental platform, and one side clamp arm of the positioning clamp is fixed to the vertical plate. The clamping ends of the two clamp arms of the positioning clamp match the needle wings of the infusion needle.

[0015] Compared with the prior art, the present invention mainly achieves the following technical effects:

[0016] After fixing the animal's limbs, use the elastic force of the elastic rope to tighten the animal's head. At this time, the animal's head is fixed. Together with the anesthetic test tube installed on the experimental platform, the animal's nose can always be kept corresponding to the evaporation port of the anesthetic test tube, which improves the anesthetic effect while eliminating the need to always hold the anesthetic test tube during perfusion, greatly improving the convenience of operation. In addition, the fixation of the animal's head and limbs is also conducive to the stability of the animal during the experiment, thereby increasing the success rate of the experiment.

[0017] Compared with the prior art, other solutions of the present invention have achieved the following technical effects:

[0018] By inserting different positions of the anesthesia test tube into the clamp, the distance between the volatilization port of the anesthesia test tube and the nose of the animal can be adjusted, thereby adapting to animals of different sizes and improving the application range of the device.

[0019] The setting of the peristaltic pump improves the automation level of the overall experiment, eliminating the need to hold a syringe to complete the perfusion, thus improving the convenience of operation.

[0020] The positioning clamp can fix the infusion needle during perfusion, eliminating the need to manually hold the infusion needle, improving the convenience of operation while increasing the stability of the infusion needle, thereby increasing the success rate of the experiment. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0022] Figure 1 This is a schematic structural diagram of an animal heart perfusion device provided with multiple peristaltic pumps in Embodiment 1 of the present invention;

[0023] Figure 2 This is a schematic structural diagram of an animal heart perfusion device provided with a peristaltic pump in Embodiment 1 of the present invention;

[0024] Figure 3 This is a schematic diagram of the structure of the experimental platform in the second embodiment of the present invention;

[0025] Figure 4 A top view of the experimental platform in the second embodiment of the present invention;

[0026] Figure 5 This is a schematic diagram of the structure of the experimental platform with hidden limb fixing structures in the second embodiment of the present invention;

[0027] Figure 6 This is a schematic diagram of the structure of the limb fixing structure in the second embodiment of the present invention;

[0028] Among them, 1. anesthesia test tube; 2. elastic rope; 3. column; 4. clamp; 5. peristaltic pump; 6. flow regulator; 7. delivery hose; 8. infusion needle; 9. liquid tank; 10. liquid storage area; 11. multi-way connector; 12. vertical plate; 13. positioning clamp; 14. box cover; 15. box body; 16. through hole; 17. fixing hole; 18. clamp rod; 19. clamp groove; 20. flexible pad; 21. buckle plate; 22. latch; 23. rotating screw; 24. connecting piece; 25. first threaded section; 26. second threaded section. DETAILED DESCRIPTION

[0029] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0030] The purpose of the present invention is to provide an animal heart perfusion device to solve the problems existing in the prior art. An anesthetic test tube is installed on an experimental platform, and an elastic rope is used to fix the animal's head so that it corresponds to the volatilization port of the anesthetic test tube, thereby improving the anesthetic effect on the animal and improving the convenience of operation.

[0031] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the present invention is further described in detail below with reference to the accompanying drawings and specific embodiments.

[0032] Embodiment 1:

[0033] Please refer to Figure 1 , Figure 2As shown, an animal heart perfusion device is provided, including an experimental platform, a liquid supply unit, an anesthesia test tube 1, a limb fixing structure and a head fixing structure, wherein the liquid supply unit is mainly used to supply perfusion liquid to the animal heart, the anesthesia test tube 1 contains liquid inhalation anesthetic, the limb fixing structure and the head fixing structure are used to fix the limbs and head of the animal respectively, the anesthesia test tube 1 is installed on the experimental platform, preferably, the anesthesia test tube 1 is installed horizontally, the head fixing structure includes an elastic rope 2, the elastic rope 2 is V-shaped, and its main function is to tighten the animal's head to complete the fixation, when perfusing mice, the elastic rope 2 can be used to tighten the mouse's teeth, and the two ends of the elastic rope 2 are respectively fixed on the experimental platform, and the fixing method is: two symmetrically arranged columns 3 are arranged on the experimental platform, and the two ends of the elastic rope 2 are respectively bound to the two columns 3 , an annular groove can be provided on the column 3, and the elastic rope 2 is bound in the annular groove to prevent the elastic rope 2 from coming out along the axial direction of the column 3. Preferably, the direction of the volatilization port of the anesthesia test tube 1 and the direction of the tip of the elastic rope 2 are located in the same vertical plane. After the limbs and head of the animal are fixed, the volatilization port of the anesthesia test tube 1 is set corresponding to the nose of the animal; the working principle of the device is: after fixing the limbs of the animal, the elastic force of the elastic rope 2 is used to tighten the head of the animal. At this time, the head of the animal is fixed, and the anesthesia test tube 1 installed on the experimental platform can always keep the nose of the animal corresponding to the volatilization port of the anesthesia test tube 1, while improving the anesthetic effect, there is no need to always hold the anesthesia test tube 1 during perfusion, which greatly improves the convenience of operation. In addition, the fixation of the animal's head and the fixation of the limbs are also conducive to the stability of the animal during the experiment, thereby improving the success rate of the experiment.

[0034] The experimental platform is provided with a clamp 4 with elastic self-recovery ability, and its material can be plastic. The clamp 4 is provided with a clamping slot matching the anesthesia test tube 1, and the anesthesia test tube 1 is clamped in the clamping slot. Since the outer diameter of the main part of the anesthesia test tube 1 remains unchanged, the anesthesia test tube 1 can be clamped into the clamp 4 at different positions to adjust the distance between the volatilization port of the anesthesia test tube 1 and the nose of the animal, thereby adapting to animals of different sizes and improving the application range of the device.

[0035] The specific structure of the liquid supply unit includes a peristaltic pump 5, a delivery hose 7, an infusion needle 8 and a liquid tank 9. The liquid tank 9 includes at least one liquid storing area 10. The number of the liquid storing areas 10 is determined according to the amount of liquid to be infused. The liquid storing area 10 is connected to the liquid inlet pipe of the peristaltic pump 5. The liquid outlet pipe of the peristaltic pump 5 is connected to the infusion needle 8 through the delivery hose 7. The setting of the peristaltic pump 5 improves the degree of automation of the overall experiment. There is no need to hold a syringe to complete the infusion, which improves the convenience of operation.

[0036] Taking the mouse perfusion experiment as an example, it requires two perfusion liquids, namely physiological saline and paraformaldehyde, so two liquid holding areas 10 are provided, which respectively hold physiological saline and paraformaldehyde. When two or more liquid holding areas 10 are provided, there are two connection methods: first, multiple liquid holding areas 10 are respectively connected to a peristaltic pump 5, and multiple peristaltic pumps 5 are connected to the infusion needle 8 through a multi-way connector 11, and a flow regulator 6 is provided on the pipeline connecting the multi-way connector 11 and the peristaltic pump 5; second, multiple liquid holding areas 10 are connected to the liquid inlet pipe of a single peristaltic pump 5 through the multi-way connector 11, and the peristaltic pump 5 is connected to the infusion needle 8 through a delivery hose 7, and a flow regulator 6 is provided on the pipeline connecting the multi-way connector 11 and the liquid holding area 10; in the case of multiple liquid holding areas 10, the flow regulator 6 can not only adjust the flow, but also cut off the passage where it is located, and the flow regulator 6 can use a roller-type flow regulator on the infusion set.

[0037] The experimental platform is provided with a fixing device for fixing the infusion needle 8 of the liquid supply unit, and the fixing device includes a vertical plate 12 and a positioning clamp 13. The vertical plate 12 is vertically arranged on the experimental platform, and a clamping arm on one side of the positioning clamp 13 is fixed to the vertical plate 12 by bolts. The clamping ends of the two clamping arms of the positioning clamp 13 match the needle wings of the infusion needle 8. The positioning clamp 13 can fix the infusion needle 8 during infusion without manually holding the infusion needle 8, thereby improving the convenience of operation and the stability of the infusion needle 8, thereby improving the success rate of the experiment.

[0038] The vertical plate 12 can be fixed on the experimental platform by glue or bolts, or a plug-in plate can be set at the bottom of the vertical plate 12, the length of the plug-in plate is smaller than the length of the vertical plate 12, and a hole matching the plug-in plate is set on the experimental platform, and the vertical plate 12 is inserted into the hole through the plug-in plate.

[0039] In this embodiment, the experimental platform includes a box cover 14 and a box body 15. The box cover 14 is buckled on the box body 15. Through holes 16 are provided on the box cover 14 at positions corresponding to both sides of the animal's chest cavity. The through holes 16 allow the perfusion fluid and blood flowing out during perfusion to flow into the box body 15. The box body 15 collects the perfusion fluid and blood to prevent pollution of the surrounding environment. On the basis of setting the box body 15 and the box cover 14, the limb fixing structure includes a fixed needle and a fixed hole 17. The box cover 14 is provided with a fixed hole 17 matching the fixed needle. The fixed needle is inserted into the fixed hole 17 after passing through the animal. A limiting block is provided at the top of the needle body of the fixed needle to facilitate the application of force to insert the needle body into the animal body. The fixed needle can be a syringe needle.

[0040] In order to adapt to animals of different sizes, multiple fixing holes 17 are provided at the position corresponding to each limb of the animal, and the multiple fixing holes 17 are arranged in sequence along the length direction of the animal. In this way, during actual fixation, the fixing needle can be inserted into the fixing hole 17 corresponding to the position of the animal's limb, thereby improving the applicability of the device.

[0041] In actual use, the limbs of the anesthetized animal are fixed on the box cover 14 by fixing the needle, and the head is fixed by the elastic rope 2. The anesthesia test tube 1 is then inserted into the clamp 4. Then the animal's chest cavity is dissected, the infusion needle 8 is inserted into the animal's left ventricle, and the infusion needle 8 is positioned using the positioning clamp 13. At this time, the peristaltic pump 5 can be turned on for perfusion operation.

[0042] Embodiment 2:

[0043] Please refer to Figures 3 to 6 As shown, the difference between this embodiment and embodiment one lies in the limb fixing structure. The limb fixing structure in this embodiment includes a clamping rod 18 and a limiting structure. The length direction of the clamping rod 18 is parallel to the length direction of the animal's body. One end of the clamping rod 18 is rotatably set above the experimental platform, and the other end is connected to the limiting structure for fixing the end of the clamping rod 18. The end of the clamping rod 18 close to the experimental platform is provided with a clamping groove 19 for squeezing the forelimbs and hind limbs of one side of the animal on the experimental platform. A flexible pad 20 for protecting the animal's limbs is provided in the clamping groove 19. The material of the flexible pad 20 can be silicone, which is connected to the clamping groove 19 by glue. Due to the deformable characteristics of the flexible pad 20, the clamping groove 19 can adapt to the forelimbs and hindlimbs of different animals within a certain range.

[0044] The clamping groove 19 may also be omitted, and the flexible pad 20 may be directly arranged at the bottom of the clamping rod 18 . In this case, the distance between the clamping rod 18 and the experimental platform will be increased.

[0045] The limiting structure can be: a matching structure of a buckle plate 21 and a pin 22, specifically, the end of the clamp rod 18 is rotatably connected to the buckle plate 21, a socket is provided on the buckle plate 21, and a pin 22 is provided on the side wall of the experimental platform, and the pin 22 is inserted into the socket to complete the fixation of the end of the clamp rod 18; it can also be: a matching structure of a buckle plate 21 and a pin 22, specifically, the end of the clamp rod 18 is rotatably connected to the buckle plate 21, a pin 22 is provided on the buckle plate 21, and a socket is provided on the side wall of the experimental platform, and the pin 22 is inserted into the socket to complete the fixation of the end of the clamp rod 18; it can also be: a matching structure of a ring-shaped telescopic rope and a binding column, specifically, a binding column is provided on the side wall of the experimental platform, one end of the ring-shaped telescopic rope is sleeved on the binding column, and the other end is sleeved on the end of the clamp rod 18 to complete the fixation of the end of the clamp rod 18, and the ring-shaped telescopic rope is in a stretched state when the two ends are respectively sleeved on the binding column and the clamp rod 18.

[0046] The fixing structure also includes a rotating screw 23 and a connecting member 24. The rotating screw 23 is rotatably set on the connecting member 24. The connecting member 24 is connected to the side of the experimental platform away from the pin 22. The rotating screw 23 includes a first thread segment 25 and a second thread segment 26 with opposite threads. The first thread segment 25 and the second thread segment 26 are respectively threadedly connected to the clamping rod 18. When the rotating screw 23 rotates, the opposite or back movement of the two clamping rods 18 can be completed by manually limiting the rotation of the clamping rod 18, thereby adjusting the distance between the two clamping rods 18, thereby adapting to animals of different sizes.

[0047] When the rotating screw 23 is set, since the position of the clamp rod 18 will change, it is necessary to set multiple pins 22 side by side on the side wall of the experimental platform to cooperate with the sockets on the buckle plate 21; or set multiple sockets side by side on the experimental platform to cooperate with the pins 22 on the buckle plate 21; the arrangement direction of the multiple pins 22 or the multiple sockets is parallel to the opposite or reverse movement direction of the two clamp rods 18.

[0048] The connector 24 can be snap-connected to the experimental platform, threadedly connected, etc., or the connector 24 can be directly integrated with the experimental platform.

[0049] In actual use, the limbs of the anesthetized animal are fixed to the box cover 14 by the clamp rod 18, the head is fixed by the elastic rope 2, and then the anesthesia test tube 1 is clamped into the clamp 4. Then the animal's chest cavity is dissected, the infusion needle 8 is inserted into the animal's left ventricle, and the infusion needle 8 is positioned using the positioning clamp 13. At this time, the peristaltic pump 5 can be turned on for perfusion operation.

[0050] Adaptive changes made according to actual needs are all within the protection scope of the present invention.

[0051] It should be noted that it is obvious to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be regarded as exemplary and non-restrictive from any point of view, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims are included in the present invention. Any reference numerals in the claims should not be regarded as limiting the claims involved.

[0052] The present invention uses specific examples to illustrate the principles and implementation methods of the present invention. The above examples are only used to help understand the method and core ideas of the present invention. At the same time, for those skilled in the art, according to the ideas of the present invention, there will be changes in the specific implementation methods and application scope. In summary, the content of this specification should not be understood as limiting the present invention.

Claims

1. An animal heart perfusion device, characterized in that: The invention comprises an experimental platform, a liquid supply unit for supplying perfusion liquid to the animal's heart, an anesthesia test tube containing liquid inhalation anesthetic, a limb fixing structure for fixing the animal's limbs, and a head fixing structure for fixing the animal's head. The anesthesia test tube is installed on the experimental platform. The head fixing structure comprises a V-shaped elastic rope for tightening the animal's head. Both ends of the elastic rope are respectively fixed on the experimental platform. The volatilization port of the anesthesia test tube is arranged corresponding to the nose of the animal.

2. The animal heart perfusion device according to claim 1, characterized in that: The experimental platform is provided with a clamping piece with elastic self-restoring ability, the clamping piece is provided with a clamping slot matching the anesthesia test tube, and the anesthesia test tube is clamped in the clamping slot.

3. The animal heart perfusion device according to claim 1, characterized in that: The liquid supply unit includes a peristaltic pump, a delivery hose, an infusion needle and a liquid tank. The liquid tank includes at least one liquid storage area, the liquid storage area is connected to the liquid inlet pipe of the peristaltic pump, and the liquid outlet pipe of the peristaltic pump is connected to the infusion needle through the delivery hose.

4. The animal heart perfusion device according to claim 3, characterized in that: When there are two or more liquid holding areas, the multiple liquid holding areas are respectively connected to one peristaltic pump, the multiple peristaltic pumps are connected to the infusion needles through a multi-way connector, and a flow regulator is provided on the pipeline connecting the multi-way connector and the peristaltic pump; or the multiple liquid holding areas are connected to the liquid inlet pipe of a single peristaltic pump through a multi-way connector, the peristaltic pump is connected to the infusion needle through the delivery hose, and a flow regulator is provided on the pipeline connecting the multi-way connector and the liquid holding areas.

5. The animal heart perfusion device according to claim 1, characterized in that: The experimental platform comprises a box cover and a box body, wherein the box cover is buckled on the box body, and through holes for perfusion fluid and blood to flow into the box body are provided on the box cover at positions corresponding to both sides of the animal's chest cavity.

6. The animal heart perfusion device according to claim 5, characterized in that: The limb fixing structure comprises a fixing needle and a fixing hole. The box cover is provided with the fixing hole matching with the fixing needle. The fixing needle is inserted into the fixing hole after passing through the animal.

7. The animal heart perfusion device according to claim 6, characterized in that: A plurality of the fixing holes are arranged corresponding to the position of each limb of the animal, and the plurality of the fixing holes are arranged in sequence along the length direction of the animal.

8. The animal heart perfusion device according to claim 1, characterized in that: The fixing structure includes a clamping rod and a limiting structure. The length direction of the clamping rod is parallel to the length direction of the animal's body. One end of the clamping rod is rotatably arranged above the experimental platform, and the other end is connected to the limiting structure for fixing the end of the clamping rod. The end of the clamping rod close to the experimental platform is provided with a clamping groove for squeezing the forelimbs and hind limbs of one side of the animal onto the experimental platform, and a flexible pad for protecting the animal's limbs is provided in the clamping groove.

9. The animal heart perfusion device according to claim 8, characterized in that: The fixing structure also includes a rotating screw and a connecting piece, wherein the rotating screw is rotatably arranged on the connecting piece, and the connecting piece is connected to a side of the experimental platform away from the pin, and the rotating screw includes a first thread segment and a second thread segment with opposite threads, and the first thread segment and the second thread segment are respectively threadedly connected to the clamping rod.

10. The animal heart perfusion device according to claim 1, characterized in that: The experimental platform is provided with a fixing device for fixing the infusion needle of the liquid supply unit, and the fixing device includes a vertical plate and a positioning clamp. The vertical plate is vertically arranged on the experimental platform, and a clamping arm on one side of the positioning clamp is fixed on the vertical plate. The clamping ends of the two clamping arms of the positioning clamp match the needle wings of the infusion needle.

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