An ant bait for use in oak forests and its preparation method

Abstract

The present invention belongs to the technical field of veterinary drugs and relates to an ant bait for use in oak forests and a preparation method thereof. The ant bait comprises the following raw materials in parts by mass: 0.01 part to 0.1 part of an insecticide, 30 parts of an attractant, 20 parts to 24 parts of an adhesive, 0.6 part to 1.2 parts of starch, 0.1 part of a preservative, 43.6 parts to 48.29 parts of a base material, and 1 part of corn oil. The prepared ant bait has a good attracting effect, and the insecticide in the bait is firmly combined with other components, reducing the impact on the oak forest environment while ensuring the efficacy of the drug.

Description

Technical Field

[0001] The invention belongs to the technical field of veterinary drugs and relates to an ant bait used in oak orchards and a preparation method thereof. Background Art

[0002] Tussah silkworms are domesticated wild silkworms that are released in wild tussah orchards. It takes nearly two months for them to hatch from eggs into larvae, molt four times, and spin silk cocoons. During this period, tussah silkworms are often attacked by various enemies. In addition to rodents and birds, various predatory insect pests in tussah orchards are the main factors affecting the stable development of the tussah silk industry. Among them, the silkworm-eating ants in tussah orchards bite and feed on tussah silkworms, and are one of the main pests of tussah silkworms. There are many types of ants that harm silkworms, mainly 9 species: yellow mound ants [Lasiusflavus(Fabricius)], black brown grass ants (Lasiusniger), reddish brown forest ants (Formica rufa), blood red forest ants (Formicasanguinea), silky forest ants (Formica fusca), Japanese bow-backed ants (Camponotusjaponicus), wrinkled red ants (Myrmicaruginodis), small yellow house ants (Pharaoh ants) (Monomoriumpharaonis L.), and black grass ants (LasiusfuliginosusLatr.); they are widely distributed, and have caused damage in major production areas such as Liaoning, Jilin, Heilongjiang, Hebei, Shandong, Henan and Inner Mongolia, seriously affecting tussah silkworm production and the efficient utilization of tussah orchard resources. There are many kinds of ants that harm silkworms. During the period of stocking tussah silkworms, the use of control agents to control ants that harm silkworms in tussah gardens is an indispensable measure for protecting tussah silkworms. Chemical agents have always been used to control ants that harm silkworms. In the early days, 666 powder was used for killing, which had good control effects, but the tussah silkworms were severely poisoned and chlorine preparations were not easy to decompose and seriously polluted the environment. Later, it was improved to spray powders with organophosphorus insecticides such as phoxim, fenitrothion, and phosmet on the ground for control, or 15 days before stocking tussah silkworms, 2kg of 40% oxydemeton-methyl emulsifiable concentrate and 3kg of water were used for ultra-low volume spraying on the ground and tussah trees for control, and 2-3 days before stocking tussah silkworms, 500-1000 times of 25% phosmet emulsifiable concentrate was used for spraying on tussah trees where ants were active. At present, the general method of prevention and control is to spread 1% phoxim powder. The specific method of application is to use a duster or a gauze bag to spread the medicine while digging ant holes before releasing the tussah silkworms. This method can effectively control the damage caused by ants that harm silkworms, but there are the following problems: the surface of phoxim powder changes color (photolysis) after exposure to the sun and is easily ineffective, resulting in a short effective period of a single application; it is easily washed away by rainwater; multiple applications are required, which can easily cause environmental pollution in the tussah garden and easily generate dust, which poses a risk of pesticide poisoning to those who apply the medicine.

[0003] At present, physical control, biological control, chemical control and other methods are used to control harmful ants at home and abroad. Among them, chemical control is an important control measure to kill harmful ants. At present, bait control is mainly adopted. The content of chemical pesticides in the bait is low. During use, the dosage is less than that of contact insecticidal powder, and the target of the medicament is strong. It is safe for most non-target organisms and highly environmentally friendly, and can well overcome the disadvantages of the powder. However, the current bait has a general attracting effect, and the binding firmness between the insecticide and other components in the bait is general, which will have a certain impact on the oak orchard environment. Summary of the Invention

[0004] In view of the above technical problems, the present invention provides an ant bait for use in an oak orchard and a preparation method thereof. The ant bait prepared by the present invention has a good attracting effect, and the binding firmness between the insecticide and other components in the bait is strong, which reduces the impact on the oak orchard environment while ensuring the efficacy.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions.

[0006] An ant bait for use in an oak orchard, comprising the following raw materials in parts by mass: 0.01 part to 0.1 part of an insecticide, 30 parts of an attractant, 20 parts to 24 parts of an adhesive, 0.6 part to 1.2 parts of starch, 0.1 part of a preservative, 43.6 parts to 48.29 parts of a substrate, and 1 part of corn oil.

[0007] Preferably, 0.02 part of an insecticide, 30 parts of an attractant, 22 parts of an adhesive, 0.8 part of starch, 0.1 part of a preservative, 46.08 parts of a substrate, and 1 part of corn oil.

[0008] Preferably, 0.02 part of an insecticide, 30 parts of an attractant, 24 parts of an adhesive, 1 part of starch paste, 0.1 part of a preservative, 43.88 parts of a substrate, and 1 part of corn oil.

[0009] Preferably, 0.1 part of an insecticide, 30 parts of an attractant, 22 parts of an adhesive, 0.8 part of starch paste, 0.1 part of a preservative, 46 parts of a substrate, and 1 part of corn oil.

[0010] Preferably, 0.1 part of an insecticide, 30 parts of an attractant, 24 parts of an adhesive, 1 part of starch paste, 0.1 part of a preservative, 43.8 parts of a substrate, and 1 part of corn oil.

[0011] Further, the insecticide is one of dinotefuran, fipronil, chlorpyrifos, spinosad, and spinetoram.

[0012] Further, the attractant is a combination of egg yolk powder, oak silkworm pupa powder, yeast extract powder, and a sweetener.

[0013] Further, the mass ratio of egg yolk powder, oak silkworm pupa powder, yeast extract powder, and the sweetener is 3 to 5: 1 to 2: 1 to 3: 2 to 4.

[0014] Further, the sweetener is one of maltose, brown sugar or sucrose.

[0015] Further, the binder is polyvinylpyrrolidone (PVP) and wheat flour.

[0016] Further, the mass ratio of polyvinylpyrrolidone (PVP) to wheat flour is 1-3:7-9.

[0017] Further, the starch is corn starch.

[0018] Further, the preservative is potassium sorbate.

[0019] Further, the base material is corn flour.

[0020] A preparation method of an ant bait for use in oak forests comprises the following steps:

[0021] (1) Dissolve the insecticide in an appropriate amount of organic solvent and mix it evenly with an appropriate amount of base material, and then dry it in a fume hood to make a mother powder containing 5 wt% of the insecticide;

[0022] (2) Weigh the mother powder, wheat flour, polyvinylpyrrolidone (PVP), and the remaining base material, and mix them evenly with a high-speed grinder to obtain mixture 1;

[0023] (3) Add the attractant to the mixture in step (2) and continue to mix evenly to obtain mixture 2;

[0024] (4) First dissolve the preservative in an appropriate amount of cold water, then add corn starch and stir evenly, and pour in boiling water to prepare a 10 wt% starch slurry;

[0025] (5) Slowly add the 10 wt% starch slurry in step (4) to the mixture 2 in step (3) under stirring conditions and mix evenly to form a dough to obtain a doughy bait;

[0026] (6) Add corn oil to the doughy bait in step (5) and mix evenly to obtain a crude bait;

[0027] (7) Dry the crude bait in step (6) so that the moisture content of the material is less than 10%, and it can form a ball when held in the hand and fall apart when the hand is loosened to obtain a dried bait;

[0028] (8) Granulate the dried bait with a 16-mesh sieve of a rocking granulator, and screen the obtained granules through a 30-mesh sieve to remove fine particles to obtain the bait.

[0029] Further, the organic solvent is one of xylene, acetone or methanol.

[0030] Further, in step (7), the drying conditions are: temperature 60 °C, time 20 min - 40 min.

[0031] Compared with the prior art, the beneficial effects of the present invention are as follows.

[0032] The bait prepared by the present invention has a good attracting effect on the ant pests in the oak orchard. Among them, the attractant is crucial, which can efficiently attract worker ants to feed, carry the bait back to the nest and then kill all the ant pests in the nest; it is more labor-saving and time-saving than the traditional method of controlling ant pests in the oak orchard; due to the existence of a specific binder, the insecticide in the bait is firmly combined with other components to avoid the loss of active ingredients, while ensuring the efficacy of the drug, it reduces the impact on the oak orchard environment. Detailed implementation manners

[0033] The technical solutions of the present invention will be clearly and completely described below in conjunction with specific embodiments. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0034] An ant bait for use in an oak orchard, comprising the following raw materials in parts by mass: 0.01 part to 0.1 part of an insecticide, 30 parts of an attractant, 20 parts to 24 parts of a binder, 0.6 part to 1.2 parts of corn starch, 0.1 part of potassium sorbate as a preservative, 43.6 parts to 48.29 parts of corn flour as a base material, and 1 part of corn oil.

[0035] Further, the insecticide is one of dinotefuran, fipronil, chlorpyrifos, spinosad, and spinetoram.

[0036] Further, the attractant is a combination of egg yolk powder, oak silkworm pupa powder, yeast extract powder, and a sweetener.

[0037] Further, the mass ratio of egg yolk powder, oak silkworm pupa powder, yeast extract powder, and the sweetener is 3 to 5: 1 to 2: 1 to 3: 2 to 4.

[0038] Further, the sweetener is one of maltose, brown sugar, or sucrose.

[0039] Further, the binder is polyvinylpyrrolidone (PVP) and wheat flour.

[0040] Further, the mass ratio of polyvinylpyrrolidone (PVP) to wheat flour is 1 to 3: 7 to 9.

[0041] A preparation method of an ant bait for use in an oak orchard, comprising the following steps:

[0042] (1) Dissolve the insecticide in an appropriate amount of organic solvent and mix it evenly with an appropriate amount of base material, and then dry it in a fume hood to make a mother powder containing 5 wt% of the insecticide;

[0043] (2) Weigh the mother powder, wheat flour, polyvinylpyrrolidone (PVP), and the remaining base materials, and mix them evenly with a high-speed pulverizer to obtain mixture 1;

[0044] (3) Add the attractant to the mixture in step (2) and continue to mix evenly to obtain mixture 2;

[0045] (4) First dissolve the preservative in an appropriate amount of cold water, then add corn starch and stir evenly, and pour in boiling water to prepare a 10wt% starch paste;

[0046] (5) Slowly add the 10wt% starch paste in step (4) and mixture 2 in step (3) under stirring conditions, and mix them evenly to form a dough to obtain the dough bait;

[0047] (6) Add corn oil to the dough bait in step (5) and mix evenly to obtain the crude bait;

[0048] (7) Dry the crude bait in step (6), and the drying conditions are: temperature 60°C, time 20 min to 40 min, so that the moisture content of the material is less than 10%, and it can form a ball when held in the hand and disperse when the hand is loosened, to obtain the dried bait;

[0049] (8) Pelletize the dried bait with a 16-mesh sieve of a rocking granulator, and screen the obtained pellets through a 30-mesh sieve to remove fine particles to obtain the bait agent.

[0050] Preferably, the organic solvent is one of xylene, acetone or methanol.

[0051] The attracting effect is evaluated by the control effect on the silkworm-eating ants in the oak orchard. In the oak orchard with serious ant damage, two application methods are adopted: evenly spreading in a ring on the surface of the ant nest (hereinafter referred to as spreading) and dotting in piles near the ant nest entrance (hereinafter referred to as dotting). The application amount per nest is 20 g, and the silkworm-eating ants are the common yellow mound ants. An evaluation test on the attracting and controlling effect of the bait agent is carried out. Using the bait method, the number of worker ants moving on the surface of the ant nest is investigated by the fresh grape pulp trapping method before application and 1 d, 4 d, 7 d, and 10 d after application, and the control effect of the agent on the worker ants is statistically analyzed.

[0052] Calculation formula: Attracting and controlling effect (%) = (Number of worker ants before application - Number of worker ants after application) / Number of worker ants before application × 100.

[0053] The adsorption effect: Baits with the same insecticide content were prepared using different binder combinations. The insecticide content in the baits was determined under the same conditions by high-performance liquid chromatography, and the adsorption and fixation of other components in the baits on the insecticide were evaluated based on the detected amount of the insecticide. The extraction of the insecticide in the bait was carried out by organic solvent extraction. The specific method was to weigh 4.0 g (accurate to 0.0002 g) of the bait to be tested and place it in a 250 mL Erlenmeyer flask, add 200 mL of acetonitrile, shake for 30 min, centrifuge at 5000 r / min for 20 min, evaporate the supernatant to near dryness on a rotary evaporator, make up the volume to 50 mL with acetonitrile, perform ultrasonic degassing, and filter through a 0.45 μm filter membrane for further testing.

[0054] Calculation formula: The mass fraction X (%) of the insecticide in the bait = (A2 × m1 × p) / (A1 × m2). Where A1 is the average peak area of the insecticide in the standard solution; A2 is the average peak area of the insecticide in the bait solution; m1 is the mass of the standard, in grams (g); m2 is the mass of the bait, in grams (g); P is the mass fraction of the insecticide standard; the difference between the results of two parallel determinations should not be greater than 0.03%, and the arithmetic mean is taken as the determination result.

[0055] Example 1.

[0056] Prepare an ant bait for use in oak forests:

[0057] (1) Dissolve 5.1 g of 98% spinetoram technical in 30 mL of methanol, mix it evenly with 94.9 g of the base material, and dry it in a fume hood to make a mother powder containing 5 wt% spinetoram.

[0058] (2) Weigh 4 g of the mother powder, 180 g of wheat flour, 40 g of polyvinylpyrrolidone (PVP), and 457 g of the base material according to the component ratio, and mix them evenly with a high-speed grinder to obtain mixture 1.

[0059] (3) Add an attractant (120 g of egg yolk powder, 30 g of oak silkworm pupa powder, 60 g of yeast extract powder, 90 g of brown sugar) to the mixture 2 in step (2) and continue to mix evenly to obtain mixture 2.

[0060] (4) First, dissolve 1 g of potassium sorbate in 12 g of cold water, then add 8 g of corn starch and stir evenly, and pour into 60 g of boiling water to prepare a 10 wt% starch paste.

[0061] (5) Slowly add the starch paste in step (4) and the mixture 2 in step (3) under stirring conditions and mix evenly to form a dough to obtain the dough bait.

[0062] (6) Add 10 g of corn oil to the dough bait in step (5) and mix evenly to obtain the crude bait.

[0063] (7) Dry the bait rough material from step (6) at 60 °C until the moisture content of the material is less than 10%, that is, it can form a ball when held by hand and disperse when the hand is loosened, to obtain the dried bait;

[0064] (8) Pelletize the dried bait with a 16-mesh sieve of a rocking granulator, and pass the obtained pellets through a 30-mesh sieve to remove fine particles to obtain a 0.02 wt% spinetoram bait.

[0065] Result: Conduct an induction and control test on the 0.02 wt% spinetoram bait prepared in Example 1. The results are shown in Table 1. The control effect of the bait on silkworm ants increases with the increase of the application treatment time. And the effect can reach more than 65% 4 days after application, and more than 82% 7 days after application. After the bait is dispersed, the control effect is higher than that of the bait spot application because it is convenient for worker ants to carry it back to the nest. Detect the prepared 0.02 wt% spinetoram bait. The detected amount of spinetoram in the bait is only 0.0071%, which is much lower than the actual content, indicating that other components in the bait have a good adsorption and fixation effect on the insecticide.

[0066] Table 1 Control effect of 0.02 wt% spinetoram bait on yellow mound ants in oak orchards

[0067]

[0068] Example 2.

[0069] Prepare an ant bait for use in oak orchards:

[0070] (1) Dissolve 5.15 g of 97% fipronil technical in 30 ml of acetone and mix it with 94.85 g of the substrate, then dry it in a fume hood to make a mother powder containing 5 wt% fipronil;

[0071] (2) Weigh 20 g of the mother powder, 160 g of wheat flour, 60 g of polyvinylpyrrolidone (PVP), and 441 g of the substrate according to the component ratio, and mix them evenly with a high-speed grinder to obtain mixture 1;

[0072] (3) Add an attractant (150 g of egg yolk powder, 60 g of oak silkworm pupa powder, 30 g of yeast extract powder, 60 g of maltose) to the mixture in step (2) and continue to mix evenly to obtain mixture 2;

[0073] (4) First dissolve 1 g of potassium sorbate in 12 g of cold water, then add 8 g of corn starch and stir evenly, and pour it into 60 g of boiling water to prepare a 10 wt% starch paste;

[0074] (5) Slowly add the starch paste from step (4) and the mixture 2 from step (3) under stirring conditions and mix them evenly to form a dough to obtain a doughy bait;

[0075] (6) Add 10 g of corn oil to the doughy bait in step (5) and mix evenly to obtain the bait rough material;

[0076] (7) Dry the bait crude material from step (6) at 60 °C until the moisture content of the material is less than 10%, and it can be formed into a ball by hand and dispersed when the hand is loosened to obtain the dried bait.

[0077] (8) Pelletize the dried bait with a 16-mesh sieve of a rocking granulator, and pass the obtained pellets through a 30-mesh sieve to remove fine particles to obtain a 0.1 wt% fipronil bait.

[0078] Conduct an induction control test on the 0.1 wt% fipronil bait prepared in Example 2. The results are shown in Table 2. Similarly, the control effect of the bait on silkworm ants increases with the increase of the application treatment time, and can reach more than 89% 10 days after application. After the bait is dispersed, the control effect is higher than that of the bait spot application because it is convenient for worker ants to carry it back to the nest. Detect the prepared 0.1 wt% fipronil bait. The detected amount of fipronil in the bait is only 0.046%, which is much lower than the actual content, indicating that other components in the bait have a good adsorption and fixation effect on the insecticide.

[0079] Table 2 Control effect of 0.1 wt% fipronil bait on yellow mound ants in oak orchards

[0080]

[0081] Example 3.

[0082] Prepare an ant bait for use in oak orchards:

[0083] (1) Dissolve 5.1 g of 98% dinotefuran technical in 100 ml of acetone and mix it with 94.9 g of the base material, then dry it in a fume hood to make a mother powder containing 5 wt% dinotefuran.

[0084] (2) Weigh 20 g of the mother powder, 200 g of wheat flour, 40 g of polyvinylpyrrolidone (PVP), and 419 g of the base material according to the component ratio, and mix them evenly with a high-speed grinder to obtain mixture 1.

[0085] (3) Add an attractant (90 g of egg yolk powder, 60 g of oak silkworm pupa powder, 60 g of yeast extract powder, 90 g of sucrose) to the mixture in step (2) and continue to mix evenly to obtain mixture 2.

[0086] (4) First dissolve 1 g of potassium sorbate in 15 g of cold water, then add 10 g of corn starch and stir evenly, and pour into 75 g of boiling water to prepare a 10 wt% starch paste.

[0087] (5) Slowly add the starch paste from step (4) and the mixture 2 from step (3) under stirring conditions and mix them evenly to form a dough to obtain a doughy bait.

[0088] (6) Add 10 g of corn oil to the doughy bait in step (5) and mix evenly to obtain the bait crude material.

[0089] (7) Dry the bait rough material from step (6) at 60 °C until the moisture content of the material is less than 10%, that is, it can form a ball when held in the hand and disperse when the hand is loosened, to obtain the dried bait;

[0090] (8) Pelletize the dried bait using a rocking granulator with a 16-mesh sieve, and pass the obtained pellets through a 30-mesh sieve to remove fine particles to obtain a 0.1% wt dinotefuran bait.

[0091] Conduct an induction control test on the 0.1 wt% dinotefuran bait prepared in Example 3. The results are shown in Table 3. Similarly, the control effect of the bait on silkworm ants increases with the increase of the application treatment time, and can reach more than 85% 10 days after application. After the bait is dispersed, the control effect is higher than that of the bait spot application because it is convenient for worker ants to carry it back to the nest. Detect the prepared 0.1 wt% dinotefuran bait. The detected amount of dinotefuran in the bait is only 0.036%, which is much lower than the actual content, indicating that other components in the bait have a good adsorption and fixation effect on the insecticide.

[0092] Table 3 Control effect of 0.1 wt% dinotefuran bait on yellow mound ants in oak orchards

[0093]

[0094] Comparative Example 1.

[0095] Prepare an ant bait for use in oak orchards:

[0096] (1) Dissolve 5.1 g of 98% spinetoram technical in 30 ml of methanol and mix it with 94.9 g of the substrate, then dry it in a fume hood to make a mother powder containing 5 wt% spinetoram;

[0097] (2) Weigh 4 g of the mother powder, 180 g of wheat flour, 40 g of polyvinylpyrrolidone (PVP), and 457 g of the substrate according to the component ratio, and mix them evenly with a high-speed grinder to obtain mixture 1;

[0098] (3) Add an attractant (60 g of egg yolk powder, 90 g of oak silkworm pupa powder, 120 g of yeast extract powder, 30 g of brown sugar) to the mixture in step (2) and continue to mix evenly to obtain mixture 2;

[0099] (4) First dissolve 1 g of potassium sorbate in 12 g of cold water, then add 8 g of corn starch and stir evenly, and pour into 60 g of boiling water to prepare a 10 wt% starch paste;

[0100] (5) Slowly add the starch paste from step (4) and the mixture 2 from step (3) under stirring conditions and mix evenly to form a dough to obtain a doughy bait;

[0101] (6) Add 10 g of corn oil to the doughy bait in step (5) and mix evenly to obtain the bait rough material;

[0102] (7) Dry the bait rough material from step (6) at 60 °C until the moisture content of the material is less than 10%, and it can form a ball when held in the hand and disperse when the hand is loosened, obtaining the dried bait.

[0103] (8) Pelletize the dried bait using a rocking granulator with a 16-mesh sieve, and pass the obtained pellets through a 30-mesh sieve to remove fine particles to obtain a 0.02 wt% spinetoram bait.

[0104] Conduct an attraction and control test on the 0.02 wt% spinetoram bait prepared in Comparative Example 1. The results are shown in Table 4. Compared with Example 1, although the control effect of the bait on silkworm ants also increases with the increase of the application treatment time, under the same conditions, its attraction and control effect is significantly lower than that of Example 1, indicating that when the ratio of the attractant is not within the appropriate range, the trapping and feeding effect of the bait on harmful silkworm ants is significantly reduced.

[0105] Table 4 Control effect of 0.02 wt% spinetoram bait on yellow mound ants in oak orchards

[0106]

[0107] Comparative Example 2.

[0108] Prepare an ant bait for use in oak orchards:

[0109] (1) Dissolve 5.1 g of 98% spinetoram technical in 30 ml of methanol and mix it with 94.9 g of the base material, then dry it in a fume hood to make a mother powder containing 5 wt% spinetoram.

[0110] (2) Weigh 4 g of the mother powder, 180 g of wheat flour, 40 g of polyvinylpyrrolidone (PVP), and 457 g of the base material according to the component ratio, and mix them evenly with a high-speed grinder to obtain mixture 1.

[0111] (3) Add an attractant (90 g of egg yolk powder, 60 g of oak silkworm pupa powder, 75 g of yeast extract powder, 75 g of brown sugar) to the mixture in step (2) and continue to mix evenly to obtain mixture 2.

[0112] (4) First, dissolve 1 g of potassium sorbate in 12 g of cold water, then add 8 g of corn starch and stir evenly, and pour it into 60 g of boiling water to prepare a 10 wt% starch paste.

[0113] (5) Slowly add the starch paste from step (4) and mixture 2 from step (3) under stirring conditions and mix them evenly to form a dough, obtaining a doughy bait.

[0114] (6) Add 10 g of corn oil to the doughy bait in step (5) and mix evenly to obtain the bait rough material.

[0115] (7) Dry the bait rough material from step (6) at 60 °C until the moisture content of the material is less than 10%, and it can form a ball when held in the hand and disperse when the hand is loosened, obtaining the dried bait.

[0116] (8) Granulate the dried bait using a rocking granulator with a 16-mesh sieve, and pass the obtained granules through a 30-mesh sieve to remove fine particles to obtain a 0.02 wt% spinetoram bait.

[0117] The 0.02 wt% spinetoram bait prepared in Comparative Example 2 was subjected to an attraction and control test, and the results are shown in Table 5. Similarly, compared with Example 1, the attraction and control efficacy of the bait was significantly lower than that of Example 1 under the same conditions, indicating that the ratio of the attractant outside the appropriate range significantly reduced the attraction and feeding effect of the bait on harmful silkworm ants.

[0118] Table 5 Control effect of 0.02 wt% spinetoram bait on yellow mound ants in oak plantations

[0119]

[0120]

[0121] Comparative Example 3.

[0122] Prepare an ant bait for use in oak plantations:

[0123] (1) Dissolve 5.1 g of 98% spinetoram technical in 30 ml of methanol and mix it with 94.9 g of the substrate, then dry it in a fume hood to obtain a mother powder containing 5 wt% spinetoram.

[0124] (2) Weigh 4 g of the mother powder, 122.2 g of wheat flour, 97.8 g of polyvinylpyrrolidone (PVP), and 457 g of the substrate according to the component ratio, and mix them evenly with a high-speed grinder to obtain mixture 1.

[0125] (3) Add an attractant (120 g of egg yolk powder, 30 g of oak silkworm pupa powder, 60 g of yeast extract powder, 90 g of brown sugar) to the mixture in step (2) and continue to mix evenly to obtain mixture 2.

[0126] (4) First, dissolve 1 g of potassium sorbate in 12 g of cold water, then add 8 g of corn starch and stir evenly, and pour it into 60 g of boiling water to prepare a 10 wt% starch paste.

[0127] (5) Slowly add the starch paste in step (4) to the mixture 2 in step (3) under stirring conditions and mix evenly to form a dough to obtain a doughy bait.

[0128] (6) Add 10 g of corn oil to the doughy bait in step (5) and mix evenly to obtain a crude bait.

[0129] (7) Dry the crude bait in step (6) at 60 °C until the moisture content of the material is less than 10%, and it can be formed into a ball by hand and scattered when the hand is loosened to obtain a dried bait.

[0130] (8) Granulate the dried bait using a rocking granulator with a 16-mesh sieve, and pass the obtained granules through a 30-mesh sieve to remove fine particles to obtain a 0.02 wt% spinetoram bait.

[0131] The prepared 0.02 wt% spinetoram bait was tested. The detected amount of spinetoram in the bait was 0.0097%, which was lower than the actual content but significantly higher than the detected amount in Example 1, indicating that the binder ratio outside the appropriate range significantly weakened the adsorption and fixation of other components in the bait on the insecticide.

[0132] Comparative Example 4.

[0133] Prepare an ant bait for use in oak forests:

[0134] (1) Dissolve 5.1 g of 98% spinetoram technical in 30 ml of methanol and mix well with 94.9 g of the substrate, then dry in a fume hood to make a mother powder containing 5 wt% spinetoram.

[0135] (2) Weigh 4 g of the mother powder, 151.25 g of wheat flour, 68.75 g of polyvinylpyrrolidone (PVP), and 457 g of the substrate according to the component ratio, and mix well with a high-speed grinder to obtain mixture 1.

[0136] (3) Add the attractant (120 g of egg yolk powder, 30 g of oak silkworm pupa powder, 60 g of yeast extract powder, 90 g of brown sugar) to the mixture in step (2) and continue to mix well to obtain mixture 2.

[0137] (4) First dissolve 1 g of potassium sorbate in 12 g of cold water, then add 8 g of corn starch and stir well, and pour into 60 g of boiling water to prepare a 10 wt% starch paste.

[0138] (5) Slowly add the starch paste in step (4) to the mixture 2 in step (3) under stirring conditions and mix well to form a dough to obtain a doughy bait.

[0139] (6) Add 10 g of corn oil to the doughy bait in step (5) and mix well to obtain a crude bait.

[0140] (7) Dry the crude bait in step (6) at 60 °C to make the moisture content of the material less than 10%, and it can be formed into a ball when held in the hand and scattered when the hand is loosened to obtain the dried bait.

[0141] (8) Granulate the dried bait using a rocking granulator with a 16-mesh sieve, and pass the obtained granules through a 30-mesh sieve to remove fine particles to obtain a 0.02 wt% spinetoram bait.

[0142] The prepared spinetoram bait with a concentration of 0.02 wt% was detected. Similarly, the detected amount of spinetoram in the bait was 0.011%, which was lower than the actual content but significantly higher than that detected in Example 1. This indicates that when the binder ratio is not within the appropriate range, the adsorption and fixation of other components in the bait on the insecticide are significantly weakened.

Claims

1. An ant bait for use in oak orchards, characterized in that: The invention comprises the following raw materials in parts by weight: 0.01 to 0.1 parts of insecticide, 30 parts of attractant, 20 to 24 parts of adhesive, 0.6 to 1.2 parts of starch, 0.1 parts of preservative, 43.6 to 48.29 parts of base material, and 1 part of corn oil; The attractant is a combination of egg yolk powder, tussah pupa powder, yeast extract powder and sweetener, the mass ratio of egg yolk powder, tussah pupa powder, yeast extract powder and sweetener is 3-5:1-2:1-3:2-4; the sweetener is one of maltose, brown sugar or sucrose; The adhesive is polyvinyl pyrrolidone and wheat flour, and the mass ratio of polyvinyl pyrrolidone to wheat flour is 1-3:7-9.

2. The ant bait for use in oak orchards according to claim 1, characterized in that: The insecticide is one of dinotefuran, fipronil, chlorpyrifos, spinosad and spinetoram.

3. The ant bait for use in oak orchards according to claim 1, characterized in that: The starch is corn starch; the preservative is potassium sorbate; and the base material is corn flour.

4. The method for preparing ant bait for use in oak orchards according to claim 1, characterized in that: The steps include: (1) dissolving the insecticide in an appropriate amount of organic solvent and mixing with an appropriate amount of substrate, and then drying in a fume hood to prepare a master powder containing 5 wt% of the insecticide; (2) Weigh the mother powder, wheat flour, polyvinyl pyrrolidone, and other base materials and mix them evenly using a high-speed grinder to obtain a mixture 1; (3) adding an attractant to the mixture in step (2) and continuing to mix to obtain a mixture 2; (4) Dissolve the preservative in an appropriate amount of cold water, then add corn starch and stir evenly, then pour into boiling water to prepare a 10 wt% starch slurry; (5) slowly adding the 10 wt% starch slurry of step (4) and the mixture 2 of step (3) under stirring conditions, and mixing them into a mass to obtain a massed bait; (6) adding corn oil to the agglomerated bait in step (5) and mixing well to obtain a coarse bait; (7) Drying the coarse bait material in step (6) until the moisture content of the material is less than 10% and the material can be lumped together and loosened by hand, thereby obtaining dried bait; (8) The dried bait is granulated using a 16-mesh screen of an oscillating granulator, and the resulting granules are passed through a 30-mesh screen to remove fine particles to obtain an ant bait.

5. The method for preparing ant bait for use in oak orchards according to claim 4, characterized in that: The organic solvent is one of xylene, acetone or methanol.

6. The method for preparing ant bait for use in oak orchards according to claim 4, characterized in that: In the step (7), the drying conditions are: temperature 60° C., time 20 min to 40 min.

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