Bactericidal composition containing pydiflumetofen and isoflurram and application of bactericidal composition

Abstract

The invention provides a bactericidal composition containing pydiflumetofen and isoflurram and application of the bactericidal composition, active ingredients of the bactericidal composition are composed of the pydiflumetofen and the isoflurram, and the mass ratio of the pydiflumetofen to the isoflurram is (20: 1)-(1: 20). After the two components are combined for use, a remarkable synergistic effect is achieved; the dosage of active ingredients can be effectively reduced, and the generation of drug resistance is slowed down.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticides, and particularly relates to a fungicide composition comprising fluopicolide and isoflucypram and an application thereof. Background Art

[0002] Fluopicolide is a pyrazole carboxamide fungicide with a mechanism of action of succinate dehydrogenase inhibitor. It affects the respiration of pathogenic fungi by inhibiting the succinate dehydrogenase in the tricarboxylic acid cycle during the mitochondrial respiration of pathogens, hinders energy metabolism, inhibits the growth of pathogens, and causes their death. It has the advantages of high efficiency, broad spectrum and low toxicity. It can be used to prevent and treat diseases caused by many pathogens such as Fusarium, Cercospora, Botrytis and Alternaria, such as wheat fusarium, rice seedling disease, cucumber powdery mildew, peanut leaf spot, apple brown spot, strawberry gray mold, rapeseed sclerotinia, potato early blight, banana black spot and citrus scab. In addition, fluopicolide can be combined with the wax layer, is resistant to rain erosion, and increases the duration of effectiveness.

[0003] Isoflucypram is a new type of succinate dehydrogenase inhibitor fungicide developed by Bayer CropScience. It has a long lasting effect and can prolong the grain filling time. It is used to prevent and control wheat rust, powdery mildew, yellow spot, leaf blight, etc., and can improve crop yield and quality.

[0004] Chemical fungicides play an important role in plant disease prevention and control, but long-term use increases drug resistance, reduces prevention efficacy, increases dosage, and pollutes the ecological environment. How to reduce the side effects of chemical pesticides is a problem that needs to be solved urgently. There are no reports on the combined use of fluopicolide and isoflurane in the prior art. The present application compounds fluopicolide and isoflurane, which can synergize within a certain mixing range, reduce costs, reduce the amount of pesticides, extend the duration of effect, and delay the development of drug resistance. Summary of the invention

[0005] In order to solve the problems existing in the prior art, the present invention provides the following technical solutions:

[0006] A fungicide composition comprising fluopicolide and isoflurane, wherein the active ingredients consist of fluopicolide and isoflurane, and the mass ratio of fluopicolide to isoflurane is 20:1-1:20.

[0007] The mass ratio of fluopicolide and isoflucypram in the bactericidal composition is 1:1-1:20.

[0008] The mass ratio of the bactericidal composition fluopicolide and isoflucypram is 1:1-1:10

[0009] The bactericidal composition preparation is a wettable powder, a water-dispersible granule or a suspension; the mass percentage of the active ingredient in the preparation is 1-75%.

[0010] The suspension contains the following ingredients in percentage by weight: 1-10% of fluopicolide, 1-20% of isoflucypram, 1-5% of Morwet EFW, 1-5% of Tersperse 4894, 1-5% of wood calcium, 0.1-0.5% of xanthan gum, 0.1-0.5% of aluminum magnesium silicate, 0.1-1% of organic bentonite, and deionized water is supplemented to 100%.

[0011] The preferred suspension contains the following ingredients in percentage by weight: 10% fluopicolide, 10% isoflurane, 2.5% Morwet EFW, 42% Tersperse 489, 2.5% wood calcium, 0.2% xanthan gum, 0.1% aluminum magnesium silicate, 0.5% organic bentonite, and deionized water to make up to 100%.

[0012] The fungicide composition is used for preventing and controlling crop diseases, especially for preventing and controlling wheat scab.

[0013] By adopting the above technical solution, the present invention has the following beneficial effects:

[0014] When the mass ratio of fluopicolide to isoflucypram is 1:1-1:20, the composition exhibits a significant synergistic effect on wheat fusarium head blight, can effectively reduce the dosage of active ingredients used, and slow down the generation of drug resistance.

[0015] The fluopicolide and isoflurane of the present invention have good field control effects on wheat fusarium head blight, and when the active ingredient dosage is 10 g / mu, the occurrence of wheat fusarium head blight can be effectively controlled. In particular, the combination of surfactant Morwet EFW, Tersperse 4894 and wood calcium in the formulation of the suspension agent of agent 1 can significantly improve the field control effect of the suspension agent. DETAILED DESCRIPTION

[0016] The present invention is further explained below in conjunction with specific implementation modes.

[0017] Example 1 Indoor toxicity determination of fluopicolide and isoflucypram combination against wheat fusarium head blight

[0018] Test pathogen: Wheat fusarium wilt, collected in the field and separated and stored for later use.

[0019] Test agents: Fluoxetine hydroxylamide technical drug and isoflucypram technical drug, commercially available.

[0020] Test method: The mycelium growth rate method was used to determine the inhibitory effects of the two agents and their compound agents. Based on the preliminary experiment, 7 drug-containing culture media with different gradient concentrations were prepared and set as controls. A 5mm diameter cake of wheat fusarium was inoculated into the center of the drug-containing culture medium and cultured for 6 days, with 4 repetitions. The colony diameter of each treatment was determined by the cross method, and the inhibition rate was calculated.

[0021] Inhibition rate = (colony diameter of control group - colony diameter of treatment group) / colony diameter of control group × 100%; the diameters in the formula are all growth diameters.

[0022] Evaluation method: The combined effects of active ingredients were evaluated according to Sun Yunpei method.

[0023] When CTC < 80, it is antagonistic;

[0024] When CTC>120, it is a synergistic effect;

[0025] When 80≥CTC≤120, it is an additive effect.

[0026] Measurement results:

[0027] As shown in Table 1, both fluopicolide and isoflurane can be used to control wheat fusarium head blight. Compared with isoflurane, fluopicolide has a higher control effect on wheat fusarium head blight. 50 The content of fluopicolide and isoflurane is 0.1025 mg / L. After the combination of fluopicolide and isoflurane, the composition exhibits different combined effects on wheat scab under different ratios. When the mass ratio of fluopicolide and isoflurane is 1:1-1:20, the composition exhibits a significant synergistic effect on wheat scab, which can effectively reduce the dosage of active ingredients and slow down the development of drug resistance.

[0028] Table 1 Indoor toxicity test of fluopicolide and isoflurane against wheat fusarium head blight

[0029]

[0030] Example 2 Field efficacy determination of fluopicolide and isoflucypram against wheat fusarium head blight Experimental crop diseases: wheat fusarium head blight;

[0031] Test drug:

[0032] 120% fluopicolide and isoflucypram suspension

[0033] The invention comprises the following ingredients in percentage by weight: 10% of fluopicolide, 10% of isoflurane, 2.5% of Morwet EFW, 2% of Tersperse 48942%, 2.5% of wood calcium, 0.2% of xanthan gum, 0.1% of aluminum magnesium silicate, 0.5% of organic bentonite, and the content is 100% with deionized water.

[0034] Pharmacy 220% fluopicolide and isoflurane suspension

[0035] The invention comprises the following ingredients in percentage by weight: 10% of fluopicolide, 10% of isoflurane, 5% of Morwet EFW, 2% of Tersperse 489, 0.2% of xanthan gum, 0.1% of magnesium aluminum silicate, 0.5% of organic bentonite, and the content is made up to 100% by deionized water.

[0036] Pharmacy 320% fluopicolide and isoflurane suspension

[0037] The invention comprises the following ingredients in percentage by weight: 10% of fluopicolide, 10% of isoflurane, 3% of MorwetEFW, 4% of wood calcium, 0.2% of xanthan gum, 0.1% of magnesium aluminum silicate, 0.5% of organic bentonite, and the content of the ingredients is 100% with deionized water.

[0038] Agent 430% fluopicolide and isoflucypram suspension

[0039] The invention comprises the following ingredients in percentage by weight: 5% of fluopicolide, 25% of isoflurane, 3% of Morwet EFW, 41% of Tersperse 489, 4% of wood calcium, 0.1% of xanthan gum, 0.15% of aluminum magnesium silicate, 0.5% of organic bentonite, and the deionized water makes up to 100%.

[0040] Pharmacy 522% fluopicolide and isoflurane suspension

[0041] The invention comprises the following ingredients in percentage by weight: 2% of fluopicolide, 20% of isoflurane, 2% of Morwet EFW, 42% of Tersperse 489, 3% of wood calcium, 0.1% of xanthan gum, 0.1% of aluminum magnesium silicate, 0.5% of organic bentonite, and deionized water is used to make up 100%.

[0042] Test method: The test set up drug treatment and blank control treatment, each treatment was repeated 4 times, and the plot area was 45m 2, arranged in random blocks. The first stem and leaf spraying was carried out at the early stage of wheat flowering, and the second spraying was carried out after an interval of 7 days. The watering rate was 50 liters / mu, and no other pesticides were sprayed except the test pesticides.

[0043] Investigation method: The disease condition of wheat in each plot was investigated in the late milky stage. Five random sampling points were taken in each plot, with 100 ears at each point. The number of diseased ears at each level and the total number of ears were recorded, and the disease index and control effect were counted and calculated respectively.

[0044] The grading standards are:

[0045] Level 0, the whole ear is disease-free;

[0046] Level 1: the diseased ear area accounts for 1 / 4 or less of the total ear area;

[0047] Level 3: the diseased ear area accounts for 1 / 4-1 / 2 of the total ear area;

[0048] Level 5: the diseased ear area accounts for 1 / 2-3 / 4 of the total ear area;

[0049] Level 7: The diseased ear area accounts for more than 3 / 4 of the total ear area.

[0050] Calculation method:

[0051]

[0052] Test results: As shown in Table 2, the fluopicolide and isoflurane of the present invention have good field control effects on wheat scab. When the active ingredient dosage is 10 g / mu, the occurrence of wheat scab can be effectively controlled. In particular, the combination of surfactant Morwet EFW, Tersperse 4894 and wood calcium in the suspension formulation of agent 1 can significantly improve the field control effect of the suspension. Through field observation, the formulation is safe for wheat.

[0053] Table 2 Field efficacy of fluopicolide and isoflurane against wheat fusarium head blight

[0054]

[0055] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention, rather than to limit it.

Claims

1. A fungicidal composition comprising pentocyclamide and isoflurane, characterized in that: The active ingredients consist of fluopicolide and isoflurane, and the mass ratio of fluopicolide to isoflurane is 20:1-1:

20.

2. The bactericidal composition according to claim 1, characterized in that The mass ratio of fluopicolide to isoflucypram is 1:1-1:

20.

3. The bactericidal composition according to claim 2, characterized in that The mass ratio of fluopicolide to isoflucypram is 1:1-1:

10.

4. The bactericidal composition according to any one of claims 1 to 3, characterized in that The preparation of the bactericidal composition is wettable powder, water dispersible granule or suspension; the mass percentage of the active ingredient in the preparation is 1-75%.

5. The bactericidal composition according to claim 4, characterized in that The bactericidal composition is a suspension.

6. The bactericidal composition according to claim 5, characterized in that The suspension contains the following ingredients in percentage by weight: 1-10% of fluopicolide, 1-20% of isoflucypram, 1-5% of Morwet EFW, 1-5% of Tersperse 4894, 1-5% of wood calcium, 0.1-0.5% of xanthan gum, 0.1-0.5% of aluminum magnesium silicate, 0.1-1% of organic bentonite, and deionized water is supplemented to 100%.

7. The bactericidal composition according to claim 6, characterized in that The suspension contains the following ingredients in percentage by weight: 10% of fluopicolide, 10% of isoflurane, 2.5% of Morwet EFW, 2% of Tersperse 4894, 2% of wood calcium, 0.2% of xanthan gum, 0.1% of aluminum magnesium silicate, 0.5% of organic bentonite, and deionized water is used to make up to 100%.

8. Use of the fungicidal composition according to any one of claims 1 to 7 for preventing and controlling crop diseases.

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