A herbicidal composition for controlling Alternanthera philoxeroides in rice fields, and its preparation method and use

Abstract

The invention relates to the technical field of pesticides, and discloses a herbicidal composition for preventing and controlling Alternanthera philoxeroides in rice fields, a preparation method thereof and a use thereof. The herbicidal composition is composed of three main active ingredients and adjuvants, and the mass percentages of the components are: 6.00-8.00% of clofopyralid, 14.00-18.00% of promethazine, 40.00-60.00% of vanillic acid, 5.00-13.00% of adjuvants, and the rest is deionized water. The herbicidal composition of the invention is applied by stem and leaf spraying, that is, 30-45 days after rice transplanting, the herbicidal composition of the invention is diluted with 30 kg of water per mu at one time and evenly sprayed on the plant surface, and the plant control effect and biomass control effect of Alternanthera philoxeroides in rice fields 30 days and 60 days after the drug is applied are significantly better than three single agents of clofopyralid, promethazine and vanillic acid, and it is safe for rice, and the rice yield is increased by more than 10.00%, and it has the advantages of safety, high efficiency and long lasting effect.

Description

Technical Field

[0001] The invention relates to the technical field of pesticides, and in particular to a weed control composition for preventing and controlling Alternanthera philoxeroides in rice fields, and a preparation method and application thereof. Background Art

[0002] Rice is one of the most important food crops in my country, with an annual planting area of ​​about 34 million hm2. 2 . Alternanthera philoxeroides is one of the first 16 major invasive alien species to invade my country. It has strong clonal reproduction ability and ecological adaptability, and is currently widely distributed in my country's major rice-growing areas. In rice fields, Alternanthera philoxeroides not only competes with rice for nutrients, water and light, but is also a natural host for rice pests such as rice planthoppers and rice leafhoppers, causing rice to be infected with diseases and pests, often reducing rice yields by more than 30%. In recent years, Alternanthera philoxeroides has gradually evolved into one of the main malignant weeds in my country's rice fields.

[0003] In order to control Alternanthera philoxeroides, domestic and foreign scholars have carried out extensive research. For example, CN104145980A discloses a pesticide composition containing bispyribac-sodium and metsulfuron-methyl and its application, discloses two pesticide compositions and application methods for efficiently controlling Alternanthera philoxeroides; CN112806377A discloses a herbicide composition and its application in reducing and increasing the efficiency of weed control. Although these inventions have a high preventive effect on Alternanthera philoxeroides, their components contain compounds such as bispyribac-sodium and amlopyralid, which have poor safety to rice and cannot be used for the prevention and control of Alternanthera philoxeroides in rice fields. CN114145193A discloses a method for comprehensive prevention and control of the alien species Alternanthera philoxeroides, which is a comprehensive prevention and control of the alien species Alternanthera philoxeroides by planting aquatic plants such as reeds, and rice fields obviously cannot plant aquatic plants such as reeds, so they cannot be applied to rice fields.

[0004] Chemical control is one of the main measures to control Alternanthera philoxeroides in rice fields at home and abroad. At present, there are two main ways to use chemical herbicides in rice fields to control Alternanthera philoxeroides. One is to drain the fields and spray them with life-killing herbicides such as glyphosate and glufosinate before transplanting rice. However, before transplanting rice, many underground stems of Alternanthera philoxeroides have not yet sprouted and escaped killing, which often leads to outbreaks of Alternanthera philoxeroides in rice fields after transplanting. The other is to use selective herbicides such as clofopyralid and pyrazosulfuron-methyl to kill Alternanthera philoxeroides during the seedling stage or vigorous growth period after rice transplanting. However, chemical herbicides such as clofopyralid and pyrazosulfuron-methyl have low control effect on the underground stems of Alternanthera philoxeroides in rice fields, and often recur again within a short time after prevention and control. In summary, the chemical herbicides currently used to control Alternanthera philoxeroides in rice fields have the disadvantages of low control effect on its underground stems and short duration of effectiveness; in addition, the control agents are relatively single, and the long-term and large-scale use of a single herbicide can easily lead to the development of resistance in Alternanthera philoxeroides.

[0005] Flupyralid, chemical name: 4-amino-3,5-dichloro-6-fluoro-pyridine-2-oxyacetic acid; is a pyridinyloxyacetic acid systemic herbicide, and is a selective systemic herbicide. This type of herbicide causes sensitive plants to react to typical hormone herbicides, with the plants becoming deformed, twisted, and eventually dying. Existing reports at home and abroad show that flupyralid has a high control effect on the aboveground stems and leaves of Alternanthera philoxeroides, but its control effect on the underground stems of Alternanthera philoxeroides is poor.

[0006] Promethazine, chemical name: 2-methylmercapto-4,6-diisopropylamino-triazine, can enter the tissues of weeds through the roots, stems and leaves. Promethazine inhibits the photosynthesis of weeds, causing them to dry up and die. It is a systemic selective herbicide. Domestic and foreign reports show that Promethazine has a high control effect on newly germinated weeds. However, there are no reports of Promethazine used in rice fields to control Alternanthera philoxeroides.

[0007] Vanillic acid, chemical name: 4-hydroxy-3-methoxybenzoic acid; widely found in the plant kingdom, is a precursor in the biosynthesis pathway of vanillin. Existing studies have shown that vanillic acid has a strong allelopathic effect, such as -3 mol / L, vanillic acid can significantly inhibit the germination of alfalfa seeds and the growth of seedlings; however, the study also found that its ability to control weeds under a single condition is poor. There are currently no reports on the use of vanillic acid to control Alternanthera philoxeroides in rice fields, or its use as a pesticide adjuvant to control Alternanthera philoxeroides.

[0008] At present, there are no reports on the use of combinations of compounds such as clofopyralid, cypermethrin and vanillic acid to control Alternanthera philoxeroides at home and abroad. Summary of the invention

[0009] In view of the shortcomings of existing chemical herbicides in the process of controlling Alternanthera philoxeroides in rice fields, such as low control effect on its underground rhizomes, short lasting effect period, large dosage and poor safety to rice, the present invention provides a herbicidal composition for controlling Alternanthera philoxeroides in rice fields and its preparation method and use to solve the above problems. According to indoor bioassay, field efficacy test and rice safety assessment, the herbicidal composition of the present invention has the advantages of high efficacy, low dosage, long lasting effect period and strong safety to rice, and after application, the rice yield is increased by more than 10.00%.

[0010] The scheme of the present invention is:

[0011] A herbicidal composition for controlling Alternanthera philoxeroides in rice fields, characterized in that it contains three main ingredients of fluroxypyra, prometryn and vanillic acid and an adjuvant, and the mass percentage of each main ingredient and the adjuvant is:

[0012] Flupirox 6.00~8.00%;

[0013] Promethazine 14.00~18.00%;

[0014] Vanillic acid 40.00~60.00%;

[0015] Additives 5.00~13.00%;

[0016] Deionized water to 100.00 %.

[0017] As a preferred technical solution, the auxiliary agent is composed of castor oil polyoxyethylene ether, sodium dodecylbenzene sulfonate, glycerol, polyether modified trisiloxane, sodium benzoate, palm oil, magnesium aluminum silicate, and xanthan gum.

[0018] As a preferred technical solution, the auxiliary agent includes the following raw materials in percentage by mass:

[0019] Castor oil polyoxyethylene ether 1.00~3.00%;

[0020] Sodium dodecylbenzenesulfonate 1.00~3.00%;

[0021] Glycerol 1.00~3.00%;

[0022] Polyether modified trisiloxane 0.10~0.50%;

[0023] Sodium benzoate 0.10~0.50%;

[0024] Palm oil 0.50~1.00%;

[0025] Magnesium aluminum silicate 0.50~1.00%;

[0026] Xanthan gum 0.50~1.00%.

[0027] Among the above-mentioned adjuvants, castor oil polyoxyethylene ether is used as a cosolvent and emulsifier to dissolve clofopyralid, promethazine, and vanillic acid to emulsify them in water without stratification or precipitation. Sodium dodecylbenzene sulfonate is used as a wetting agent to reduce the surface tension of the liquid medicine, making it easier to spread on the leaves of plants without rolling off. Glycerol can enhance the stability of the agent and delay the degradation of the agent, while also improving the permeability and systemicity of the pesticide and enhancing the efficacy. Polyether-modified trisiloxane is used as a spreading agent to improve the adhesion and absorption efficiency of agricultural pesticides on the plant surface and enhance the efficacy. Sodium benzoate is used as a preservative to prevent the deterioration of the agent and extend the storage time of the agent. Palm oil is used as a suspending agent to prevent the agent from flocculating and precipitating during storage, so that the agent can be stored for a long time without changing its properties.

[0028] Magnesium aluminum silicate is used as an adhesive to improve the adhesion of the agent on the plant surface and prevent the agent from rolling off the leaves. Xanthan gum is used as a suspending agent and stabilizer to prevent the agent from flocculating and precipitating during storage, while improving the stability of the agent so that the agent can be stored for a long time without changing its properties.

[0029] The present invention also discloses a method for preparing a herbicidal composition for controlling Alternanthera philoxeroides in rice fields, comprising the following steps:

[0030] S1, add fluroxypyraclostrobin, prometryn and vanillic acid into reaction tank a according to the ratio, stir evenly, inject the composition into a multi-stage grinder for grinding, and discharge the ground material after 98% of the fineness passes 1-5 um, and material a is set aside;

[0031] S2, add castor oil polyoxyethylene ether, sodium dodecylbenzene sulfonate, glycerol, polyether modified trisiloxane, sodium benzoate, palm oil, magnesium aluminum silicate and xanthan gum into reaction tank b according to the proportion, and stir until they are completely dissolved;

[0032] S3, injecting material a and the material in reaction tank b into an emulsification tank, adding deionized water according to the proportion, and then high-speed shearing and emulsification to obtain a herbicidal composition for controlling Alternanthera philoxeroides in rice fields.

[0033] The present invention also discloses a use of a herbicidal composition for preventing and controlling Alternanthera philoxeroides in rice fields, which is used for preventing and controlling Alternanthera philoxeroides in rice fields. Specifically, 30 to 45 days after rice transplanting, the rice fields are drained to less than 2 cm, and then the herbicidal composition for preventing and controlling Alternanthera philoxeroides in rice fields is diluted with 30 kg of water at a rate of 100 g per mu, mixed evenly, and then sprayed on the surface of the plants on the stems and leaves at one time.

[0034] Advantages of the present invention:

[0035] (1) Compared with the three single agents of fluroxypyra, prometryn and vanillic acid, the herbicidal composition of the present invention has a significantly improved control effect on Alternanthera philoxeroides.

[0036] Indoor bioassays showed that when the dosage of clofopyralid and promethazine was 9.00 and 18.00 g ai / mu, their biomass control effects on the aboveground stems and underground rhizomes of Alternanthera philoxeroides were 71.32%, 36.06%, 36.09% and 38.39%, respectively, while the control effect of the combination of the two was 93.02% and 82.67%. The control effect of the combination on Alternanthera philoxeroides was significantly better than that of the single agent.

[0037] The field efficacy test showed that the herbicidal composition of the present invention had a plant control effect and a fresh weight control effect on Alternanthera philoxeroides at 30 days after the application, and a plant control effect and a fresh weight control effect of more than 85.00% at 60 days after the application. However, when clofopyralid was used at 20.00 g ai / mu, the plant control effect and the fresh weight control effect on Alternanthera philoxeroides were 87.54% and 91.27% at 30 days after the application, and 49.67% and 56.24% at 60 days after the application, respectively, which were lower than the herbicidal composition of the present invention. In addition, when the dosage of promethazine was 40.00 g ai / mu, the plant control effect and fresh weight control effect on Alternanthera philoxeroides were 21.41% and 28.32% at 30 days after the application, and 14.26% and 21.31% at 60 days after the application. When the dosage of vanillic acid was 100.00 g ai / mu, the plant control effect and biomass control effect on Alternanthera philoxeroides were negative at 30 days and 60 days after the application, indicating that the growth of Alternanthera philoxeroides was promoted. The research results show that the control effect of three single agents of clofopyralid, promethazine and vanillic acid on Alternanthera philoxeroides is significantly lower than that of the herbicidal composition of the present invention. Therefore, the herbicidal composition of the present invention significantly improves the plant control effect and biomass control effect of a single component on Alternanthera philoxeroides in farmland.

[0038] (2) Compared with the three single agents of fluroxypyra, prometryn and vanillic acid, the herbicidal composition of the present invention has a long duration of effectiveness in controlling Alternanthera philoxeroides.

[0039] Field efficacy tests showed that when clofopyralid, promethazine and vanillic acid were used at dosages of 20.00, 50.00 and 100 ga.i. / mu, 60 days after application, the plant control efficacy and fresh weight control efficacy of clofopyralid on Alternanthera philoxeroides were 49.67% and 56.24%, respectively, the control efficacy of promethazine was 14.26% and 21.31%, respectively, and the control efficacy of vanillic acid was -13.57% and -19.35%, respectively. The herbicidal composition of the present invention had a plant control efficacy of more than 85.00% and a fresh weight control efficacy of more than 90.00% on Alternanthera philoxeroides 60 days after application. Therefore, compared with traditional chemical agents, the herbicidal composition of the present invention significantly prolongs the duration of effect on Alternanthera philoxeroides.

[0040] (3) The herbicidal composition of the present invention significantly reduces the amount of chemical herbicides such as clofopyralid and promethazine.

[0041] In the herbicidal composition of the invention, the per-mu dosage of the chemical herbicide clofopyralid is 6-8 g, and the per-mu dosage of promethazine is 14.00-18.00 g. However, the recommended dosage of single agents clofopyralid and promethazine in rice fields is usually 20 g and 50 g, respectively. Therefore, relative to the recommended dosage of a single agent, the herbicidal composition of the present invention reduces the field dosage of clofopyralid by 60.00-70.00% and promethazine by 64.00-72.00% per mu. The cost is saved and the ecological environment is protected.

[0042] (4) The herbicidal composition of the present invention, when applied by stem and leaf spraying, significantly increases rice yield better than the three single agents of fluroxypyra, promethazine and vanillic acid.

[0043] Field tests show that the herbicidal composition of the present invention, when applied by stem and leaf spraying, has an increase rate of rice yield higher than 10.00%; while under the same conditions, the increase rates of three single agents of clofopyralid, promethazine and vanillic acid on rice yield are 5.29%, 2.69% and 0.47% respectively. The herbicidal composition of the present invention has a significantly higher yield-increasing effect on rice than three single agents of clofopyralid, promethazine and vanillic acid. DETAILED DESCRIPTION

[0044] In order to make the technical means, objectives and effects achieved by the present invention easier to understand, the present invention is further described below in conjunction with specific embodiments.

[0045] Example 1: Screening of the optimal mass ratio of fluroxypyra and promethazine for controlling Alternanthera philoxeroides

[0046] 1.1 Test materials

[0047] 20% clofopyralid, Chengdu Bangnong Chemical Co., Ltd.; 50% promethazine, Kunming Pesticide Co., Ltd.; Alternanthera philoxeroides was collected from ditches around Yunnan Academy of Agricultural Sciences.

[0048] 1.2 Test methods

[0049] The bioactivity of clofopyralid, promethazine and their combination in different proportions against Alternanthera philoxeroides was determined by pot culture under greenhouse conditions to screen the optimal ratio for the prevention and control of Alternanthera philoxeroides. 25 kg of dried and crushed paddy soil was placed in a plastic pot of 20 cm × 10 cm × 9.5 cm (length × width × height), and the soil in each plastic pot was completely moistened. The aboveground stems of the sturdy A. philoxeroides that occurred in the field that year were collected, and the stems of A. philoxeroides with 3 stem nodes, a diameter of 4-6 mm, and a length of 10-15 cm were cut after cleaning. Two of the stem nodes were buried in the soil, and only one stem node was left on the surface. The length of the surface stem was 3-5 cm, and 15 plants were planted in each pot. After planting, water was slowly added to each pot along the wall of the plastic pot until the soil was completely moistened, and then it was placed in a greenhouse for cultivation (temperature of 30℃ / 18℃, 12h light / 12h dark). After 30 days, the pesticide was applied in the spray tower according to the designed dosage of the experiment by spraying the stems and leaves (spraying amount of 30L / mu, spraying pressure of 0.6kpa), and the control was sprayed with pure water. Each treatment was repeated 4 times (4 pots). After 2 hours of application, continue to culture in the greenhouse under the same culture conditions as in the previous period.

[0050] 30 days after the application of the medicine, all the Alternanthera philoxeroides in the pots were taken out, the dead branches and leaves above the ground and the dead and rotten underground rhizomes were removed, and then the above-ground and underground rhizomes were separated, and the biomass was weighed respectively, and compared with the control, and the biological activity was calculated.

[0051] 1.3 Calculation method:

[0052] The Gowing method was used to evaluate the combined effect of two mixed drugs, and the formula is as follows:

[0053] Actual biomass inhibition rate (%) = [(control weed biomass - treatment weed biomass) / control weed biomass] × 100

[0054] Theoretical inhibition rate E0 (%) = (X + Y) - (XY / 100)

[0055] In the formula, X is the weed control effect of treatment group A relative to the control group when the dosage of herbicide A is p; Y is the weed control effect of treatment group B relative to the control group when the dosage of herbicide B is q; E0 is the theoretical control effect when the dosage of herbicide A is p + the dosage of herbicide B is q. E is the actual control effect when the dosage of herbicide A is p + the dosage of herbicide B is q.

[0056] The actual inhibition rate (E) was compared with the theoretical inhibition rate (E0). When the E-E0 value was >10%, it was a synergistic effect, when the E-E0 value was <-10%, it was an antagonistic effect, and when the E-E0 value was between -10% and 10%, it was an additive effect. All data were statistically analyzed using DPS v9.01 software, and the significance of the differences among the treatments was tested using a randomized block design combined with Duncan's new multiple range method.

[0057] Table 1 The combined effect of fluroxypyra and prometryn on the aboveground and underground fresh weight of Alternanthera philoxeroides 30 days after application

[0058]

[0059] Note: The lowercase letters in Table 1 indicate the significant difference of the data in the same column at the 5% level. The same letters indicate insignificant difference, while different letters indicate significant difference.

[0060] The research results show that the control effects of clofopyralid and promethazine on the biomass of aboveground stems and leaves and underground rhizomes of Alternanthera philoxeroides increased with the increase of concentration. When the dosage of clofopyralid was 9.00 g ai / mu, the control effects on the biomass of aboveground stems and leaves and underground rhizomes of Alternanthera philoxeroides were 71.32% and 36.06%, respectively, indicating that the control effect of clofopyralid on underground rhizomes of Alternanthera philoxeroides was poor. When the dosage of promethazine was 18.00 g ai / mu, the control effects on the biomass of aboveground stems and leaves and underground rhizomes of Alternanthera philoxeroides were 36.09% and 38.39%, respectively, indicating that there was no significant difference in the control effect of promethazine on the aboveground stems and leaves and underground rhizomes of Alternanthera philoxeroides.

[0061] The results showed that a certain amount of clofopyralid (6.00-8.00 ga.i. / mu) combined with promethazine (12.00-18.00 g ai / mu) showed a synergistic effect on the biomass of the aboveground stems and leaves and underground rhizomes of Alternanthera philoxeroides (E 实测 -E 理论 >10%), and the synergistic effect is significant. Among them, when the dosage of clofopyralid and promethazine is 6.00+16.00 g ai / mu, 6.00+18.00 g ai / mu, 7.00+16.00 g ai / mu, 7.00+18.00 g ai / mu, 8.00+14.00 g ai / mu, 8.00+16.00 g ai / mu, and 8.00+18.00 g ai / mu, the control effect on the aboveground stems and leaves of Alternanthera philoxeroides is higher than 80.00%, and the control effect on the underground rhizomes is higher than 70.00%, which can meet the requirements of modern agriculture. The control effect of the combination on the aboveground stems and leaves and underground rhizomes of Alternanthera philoxeroides is significantly higher than that of a single compound, which is the preferred option for the combination of the two to control Alternanthera philoxeroides.

[0062] Example 2: Effect of vanillic acid on the efficacy of a combination of fluroxypyra and prometryn in preventing and treating Alternaria philoxeroides

[0063] 2.1 Test materials

[0064] 20% fluroxypyra, Chengdu Bangnong Chemical Co., Ltd.; 50% prometryn, Kunming Pesticide Co., Ltd.; the combination of fluroxypyra and prometryn was prepared on site according to the results of Example 1. Vanillic acid, Chengdu Medson Technology Co., Ltd. Alternanthera philoxeroides was collected from the ditch around Yunnan Academy of Agricultural Sciences.

[0065] 2.2 Test methods

[0066] Based on the research results of Example 1, this experiment mainly tested the effects of vanillic acid at different doses on the combination of clofopyralid and promethazine (6.00+16.00 g ai / mu, 6.00+18.00 g ai / mu, 7.00+16.00 g ai / mu, 7.00+18.00 g ai / mu, 8.00+14.00 g ai / mu, 8.00+16.00 g ai / mu, 8.00+18.00 g ai / mu) in controlling Alternanthera philoxeroides. The experiment was carried out under greenhouse potted conditions.

[0067] First, the cultivation of Alternanthera philoxeroides under greenhouse conditions is carried out in the same manner as in Example 1. After the seedlings have been cultivated for 30 days, the agent is applied in the spray tower according to the designed dosage of the test, and the spray volume is 30.00 L / mu, and the spray pressure is 0.6 kpa; specifically, for the 7 preferred items of clofopyralid and promethazine in Example 1 for Alternanthera philoxeroides, the effects of vanillic acid on the prevention and control of Alternanthera philoxeroides at dosages of 20.00, 40.00, 60.00, and 80.00 g ai / mu were tested, and the blank control was not applied with the drug (see specific implementation). A total of 36 treatments were set up in the experiment, and each treatment was repeated 4 times (4 pots). After the drug was applied, each test treatment continued to be cultured in the greenhouse, and the culture conditions were the same as in the previous period. 15 days after the drug, each pot of Alternanthera philoxeroides was completely dug out, and the surviving plants were counted. Remove and clean the dead stems and leaves above ground and the dead roots below ground of the surviving plants, absorb the water with absorbent paper and weigh the fresh weight. The specific implementation series is as follows:

[0068] Series I-1 to I-7: The combination of clofopyralid and promethazine was used at a dosage of 6.00+16.00 g ai / mu, 6.00+18.00 g ai / mu, 7.00+16.00 g ai / mu, 7.00+18.00 g ai / mu, 8.00+14.00 ga.i. / mu, 8.00+16.00 g ai / mu, and 8.00+18.00 g ai / mu, respectively.

[0069] Series II-1 to II-7: Vanillic acid was used at a dosage of 20.00 g ai / mu and was mixed with 6.00+16.00 ga.i. / mu, 6.00+18.00 g ai / mu, 7.00+16.00 g ai / mu, 7.00+18.00 g ai / mu, 8.00+14.00 g ai / mu, 8.00+16.00 g ai / mu, and 8.00+18.00 g ai / mu of clofopyralid and promethazine respectively and then used.

[0070] Series III-1 to III-7: Vanillic acid was used at a dosage of 40.00 g ai / mu and was mixed with 6.00+16.00 ga.i. / mu, 6.00+18.00 g ai / mu, 7.00+16.00 g ai / mu, 7.00+18.00 g ai / mu, 8.00+14.00 g ai / mu, 8.00+16.00 g ai / mu, and 8.00+18.00 g ai / mu of clofopyralid and promethazine respectively for use.

[0071] Series IV-1 to IV-7: Vanillic acid was used at a dosage of 60.00 g ai / mu and was mixed with 6.00+16.00 ga.i. / mu, 6.00+18.00 g ai / mu, 7.00+16.00 g ai / mu, 7.00+18.00 g ai / mu, 8.00+14.00 g ai / mu, 8.00+16.00 g ai / mu, and 8.00+18.00 g ai / mu of clofopyralid and promethazine respectively.

[0072] Series V-1 to V-7 vanillic acid was used at a dosage of 80.00 g ai / mu and was mixed with 6.00+16.00 ga.i. / mu, 6.00+18.00 g ai / mu, 7.00+16.00 g ai / mu, 7.00+18.00 g ai / mu, 8.00+14.00 g ai / mu, 8.00+16.00 g ai / mu, and 8.00+18.00 g ai / mu of clofopyralid and promethazine, respectively.

[0073] 2.3 Calculation method:

[0074] Fresh weight control effect (%) = (control fresh weight - treatment fresh weight) × 100 / control fresh weight, increase efficiency (%) = control effect of adding vanillic acid (%) - control effect of not adding vanillic acid (%). The data were statistically analyzed using DPS v 9.01 software. One-way ANOVA was used to test the weed control effect of the test materials, and Duncan's new multiple range method was used for multiple comparisons of the inhibition rate to analyze the differences between different treatments.

[0075] Table 2 Effect of vanillic acid on the herbicidal effect of fluroxypyraclostrobin and promethazine combination

[0076]

[0077] Note: The lowercase letters in the table represent the significant comparison of the inhibition rate data in the same column at the 5% level. The same letters mean that the difference is not significant, otherwise, it is significant.

[0078] As shown in Table 2, different doses of vanillic acid have different effects on the control of Alternanthera philoxeroides by the mixture of clofopyralid and promethazine. When vanillic acid is 20.00 g ai / mu, the control effect of the herbicide mixture on the aboveground stems and leaves of Alternanthera philoxeroides is reduced, so it does not meet the actual needs. When the concentration of vanillic acid was 40.00 g ai / mu and 60.00 g ai / mu, its synergistic effect on the aboveground stems and leaves and underground rhizomes of Alternanthera philoxeroides was most obvious, especially when the dosage of clofopyralid, promethazine and vanillic acid was 6.00+18.00+40.00 g ai / mu, 7.00+18.00+40.00 g ai / mu, 8.00+16.00+40.00 g ai / mu, 6.00+16.00+60.00 g ai / mu, 7.00+14.00+60.00 g ai / mu and 8.00+14.00+60.00 g ai / mu, the control effect on the aboveground stems and leaves of Alternanthera philoxeroides exceeded 90.00 %, and the control effect of underground rhizomes exceeded 80.00%; although its control effect was slightly lower than that of 80.00 g ai / mu of vanillic acid, it was in line with the principle of reducing application and increasing efficiency in modern agriculture. Therefore, the per-mu application rates of clofopyralid, promethazine and vanillic acid were 6.00+18.00+40.00 ga.i, 7.00+18.00+40.00 g ai, 8.00+16.00+40.00 g ai, 6.00+16.00+60.00 g ai, 7.00+14.00+60.00 g ai and 8.00+14.00+60.00 g ai as the preferred items of the herbicide mixture.

[0079] Example 3: Preparation of a herbicidal composition containing fluroxypyraclostrobin, prometryn and vanillic acid

[0080] The proportions of the herbicidal composition suspension are calculated by mass percentage: 6.00-8.00% of clofopyralid, 14.00-18.00% of promethazine, 40.00-60.00% of vanillic acid, 1.00-3.00% of castor oil polyoxyethylene ether, 1.00-3.00% of sodium dodecylbenzene sulfonate, 1.00-3.00% of glycerol, 0.10-0.50% of polyether modified trisiloxane, 0.10-0.50% of sodium benzoate; 0.50-1.00% of palm oil, 0.5.-1.00% of aluminum magnesium silicate, 0.50-1.00% of xanthan gum, and deionized water to 100.00%.

[0081] The preparation method of the herbicidal composition suspension is as follows:

[0082] S1, add fluroxypyraclostrobin, prometryn and vanillic acid into reaction tank a according to the ratio, stir evenly, inject the composition into a multi-stage grinder for grinding, and discharge the ground material after 98% of the fineness passes 1-5um, and material a is set aside;

[0083] S2, add castor oil polyoxyethylene ether, sodium dodecylbenzene sulfonate, glycerol, polyether modified trisiloxane, sodium benzoate, palm oil, magnesium aluminum silicate and xanthan gum into reaction tank b according to the proportion, and stir until completely mixed;

[0084] S3, injecting material a and the material in reaction tank b into an emulsification tank, adding deionized water according to the proportion, and then high-speed shearing and emulsification to obtain a herbicidal composition for controlling Alternanthera philoxeroides in rice fields.

[0085] Example 4: Field efficacy test of a herbicidal composition containing fluroxypyraclostrobin, prometryn and vanillic acid

[0086] 4.1 Test materials

[0087] The tested herbicidal composition suspension was prepared according to the method of Example 3, and the tested rice variety was Yunjing 37, a main variety grown in Yunnan Province.

[0088] 4.2 Test methods

[0089] The field efficacy of the test herbicide combination on Alternanthera philoxeroides in paddy fields was tested through field plot experiments. The test field is located in Che Village, Banqiao Town, Luliang County (102.4605°E, 24.1482°N, 1528.4 meters above sea level). The test plots are divided by field ridges, and the ridges are wrapped with plastic film to prevent water from flowing between plots. The soil type is clay loam with a clay content of >90%; the soil pH is 6.3 and the organic matter content is 2.914%. The fertility is moderate, with total nitrogen of 0.257 mg / kg, total phosphorus of 0.103 mg / kg, and total potassium of 1.6 mg / kg. The test rice was sown on April 10, 2023 and transplanted on May 14, 2023. The management measures after transplanting were consistent with those in the local area, including irrigation, fertilization, and pest and disease control. Conventional management measures were adopted. No other herbicides and plant growth regulators were used during the experiment. Based on the results of Example 1 and Example 2, 30 days after rice transplanting, when Alternanthera philoxeroides fully emerged in the paddy field, stem and leaf spraying and poisoned soil spreading were used for pesticide application. A total of 16 treatments were set up in the experiment. The mass percentage ratios of compounds in different treatments (Series I, Series II, Series III, Series IV, Series V and Series VI) are shown in Table 3, and each treatment was repeated 4 times. In addition, the experiment set up 3 drug controls (Series VII, Series VIII and Series IX) and 1 blank control (spraying water or spreading soil). The methods of stem and leaf spraying and spreading poisoned soil specifically refer to the "Guidelines for Field Efficacy Tests of Pesticides" (I) Herbicides for Controlling Weeds in Paddy Fields (GBT17980.40-2000).

[0090] 4.3 Data collection and calculation

[0091] Fifteen days after application, the coverage was visually evaluated. At 30 and 60 days after application, four random sampling points (0.25 m2) were sampled diagonally in each plot. 2 / points), respectively investigating the plant number, tillering and fresh weight of Alternanthera philoxeroides.

[0092] Table 3 Composition of the tested herbicidal compositions

[0093]

[0094] 4.4 Results Analysis

[0095] Table 4 Field control effect of the herbicide combination of fluroxypyra, prometryn and vanillic acid on Alternanthera philoxeroides

[0096]

[0097] Note: The lowercase letters in the table are for comparison of data in the same column. If the numbers are different, the difference is significant (P<0.05), otherwise the difference is not significant.

[0098] The results showed that the mixture of clofopyralid, promethazine and vanillic acid had a high control effect on field Alternanthera philoxeroides when sprayed on stems and leaves, and the coverage control effect was 100% 15 days after the application. As time went on, the plant control effect and biomass control effect of the herbicidal combination on Alternanthera philoxeroides decreased; 60 days after the application, the plant control effect on Alternanthera philoxeroides was greater than 85.00%, and the biomass control effect was greater than 90.00%, and there was no significant difference in control effect between different treatments, but it was significantly higher than the control effect of a single agent. The application method through poisoned soil had a poor effect. Therefore, the results of this study showed that clofopyralid, promethazine and vanillic acid had high field control efficacy against Alternanthera philoxeroides through stem and leaf spray at a per-acre dosage of 6.00+18.00+40.00 g ai, 7.00+18.00+40.00 g ai, 8.00+16.00+40.00 g ai, 6.00+16.00+60.00 g ai, 7.00+14.00+60.00 g ai and 8.00+14.00+60.00 ga.i.

[0099] Example 5: Safety evaluation of a herbicidal composition containing fluroxypyraclostrobin, prometryn and vanillic acid on rice

[0100] 4.1 Test materials

[0101] The suspension concentrate of the herbicidal composition tested was the same as that in Example 4, and the rice variety tested was Yunjing 37, a main variety grown in Yunnan Province.

[0102] 4.2 Test methods

[0103] The test method, test treatment and implementation series were the same as those in Example 4. The safety of the drug to rice was investigated according to the 5-level classification method 3, 7, 15, 30, 45 and 60 days after application.

[0104] 5-level classification standard for pesticide damage:

[0105] Level 0: Plants grow normally without any damage symptoms; recorded as “-”;

[0106] Level 1: less than 1 / 4 of the total leaves are dead; the plant is slightly damaged by the pesticide and can recover, which is recorded as "+";

[0107] Level 2: 1 / 4 to 1 / 2 of the total leaves are dead, the plant is moderately damaged by the pesticide, but can recover later and the yield is not affected; recorded as "++";

[0108] Level 3: 1 / 2 to 3 / 4 of the total leaves are dead, the plants are severely damaged and difficult to recover, resulting in reduced yield; recorded as "+++";

[0109] Level 4: More than 3 / 4 of the total leaf area or the entire plant is dead, the plant is severely damaged by the pesticide and is difficult to recover or the plant dies; recorded as "++++".

[0110] Table 5 Safety evaluation of rice at different times after different treatments

[0111]

[0112] The results show that the herbicidal composition for the test was applied by spreading poisoned soil, and the agronomic traits of each treatment were consistent with the blank control, and no symptoms of phytotoxicity such as growth inhibition, deformity and discoloration occurred. When the herbicidal composition was applied by spraying, there was a certain amount of phytotoxicity to rice 3 days and 7 days after the application. Among them, series I and series II had moderate phytotoxicity to rice 3 days after the application, slight phytotoxicity 15 days after the application, and basically recovered 30 days after the application. The rice in other treatments also showed slight phytotoxicity 3 days after spraying, but recovered 15 days after the application. As can be seen from Example 3, the poisoned soil method has a low control effect on Alternanthera philoxeroides in rice fields. Therefore, considering the control effect of the comprehensive agent on Alternanthera philoxeroides and the safety of rice, it is recommended to use the spray method 30 days after rice transplanting.

[0113] Example 6: Effect of a herbicidal composition containing fluroxypyraclostrobin, prometryn and vanillic acid on rice growth

[0114] 5.1 Test materials

[0115] The tested herbicidal composition suspension was prepared according to the method of Example 3, and the tested rice variety was Yunjing 37, a main variety grown in Yunnan Province.

[0116] 5.2 Test methods

[0117] The test method, test treatment and implementation series are the same as in Example 3.

[0118] Table 6 Effect of herbicide combination on rice growth

[0119]

[0120] Note: The lowercase letters in the table are for comparison of data in the same industry. Different numbers indicate significant differences (P < 0.05), otherwise the differences are not significant.

[0121] As shown in Table 6, the number of seedlings, effective panicles, number of grains, thousand-grain weight and yield in the blank control treatment were 36.1, 9.5, 121.6, 26.1 grams and 725.99 kg / mu, respectively, while the effective panicles, number of grains and yield of each treatment using the spray method were significantly higher than those of the blank control, and the yield increase rate was higher than 10%, and there was no significant difference among the treatments. In addition, the yield of each treatment using the mist method was also significantly higher than that of each treatment using the poisoned soil method. Therefore, applying a mixture of clofopyralid, promethazine and vanillic acid by spraying 30 days after rice transplanting can significantly increase rice yield.

[0122] The above shows and describes the basic principles, main features and advantages of the present invention. It should be understood by those skilled in the art that the present invention is not limited to the above embodiments. The above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention. The scope of protection of the present invention is defined by the attached claims and their equivalents.

Claims

1. A herbicidal composition for controlling Alternanthera philoxeroides in rice fields, characterized in that: It contains three main active ingredients, fluroxypyrazone, prometryn and vanillic acid, adjuvants and water, and the mass percentage of each ingredient is: Flupirox 6.00~8.00%; Promethazine 14.00~18.00%; Vanillic acid 40.00~60.00%; Additives 5.00~13.00%; Deionized water to 100.00 %.

2. The herbicidal composition for controlling Alternanthera philoxeroides in rice fields according to claim 1, characterized in that: The auxiliary agent is composed of castor oil polyoxyethylene ether, sodium dodecylbenzene sulfonate, glycerol, polyether modified trisiloxane, sodium benzoate, palm oil, magnesium aluminum silicate, and xanthan gum, and the mass percentage of each component is: Castor oil polyoxyethylene ether 1.00~3.00%; Sodium dodecylbenzenesulfonate 1.00~3.00%; Glycerol 1.00~3.00%; Polyether modified trisiloxane 0.10~0.50%; Sodium benzoate 0.10~0.50%; Palm oil 0.50~1.00%; Magnesium aluminum silicate 0.50~1.00%; Xanthan gum 0.50~1.00%.

3. A method for preparing the herbicidal composition for controlling Alternanthera philoxeroides in rice fields as claimed in claim 1, characterized in that: The following steps are involved: S1, add fluroxypyraclostrobin, prometryn and vanillic acid into reaction tank a according to the ratio, stir evenly, inject the composition into a multi-stage grinder for grinding, and discharge the ground material after 98% of the fineness passes 1-5um to obtain material a for standby use; S2, add the components of the auxiliary agent into the reaction tank b according to the ratio, and stir until they are completely dissolved; S3, injecting material a and the material in reaction tank b into an emulsification tank, adding deionized water according to the proportion, and then high-speed shearing and emulsification to obtain a herbicidal composition for controlling Alternanthera philoxeroides in rice fields.

4. The method according to claim 3, characterized in that The distribution ratios of each group are as follows: Flupirox 6.00~8.00%; Promethazine 14.00~18.00%; Vanillic acid 40.00~60.00%; Castor oil polyoxyethylene ether 1.00~3.00%; Sodium dodecylbenzenesulfonate 1.00~3.00%; Glycerol 1.00~3.00%; Polyether modified trisiloxane 0.10~0.50%; Sodium benzoate 0.10~0.50%; Palm oil 0.50~1.00%; Magnesium aluminum silicate 0.50~1.00%; Xanthan gum 0.50~1.00%; Deionized water to 100.00 %.

5. Use of the herbicidal composition for controlling Alternanthera philoxeroides in rice fields as claimed in any one of claims 1 to 2 or the herbicidal composition prepared by the preparation method of the herbicidal composition for controlling Alternanthera philoxeroides in rice fields as claimed in any one of claims 3 to 4, characterized in that: For preventing and controlling Alternanthera philoxeroides in rice fields, 30 to 45 days after rice transplanting, the rice fields are drained to below 2 cm, and then 100 g of the herbicidal composition for preventing and controlling Alternanthera philoxeroides in rice fields is diluted with 30 kg of water per mu, mixed well, and then sprayed on the surface of the plants' stems and leaves once.

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