A rumen-skimming coating preparation for ruminants and its preparation method and application

Abstract

The present invention proposes a rumen-passing coating preparation for ruminants and a preparation method and application thereof. The rumen-passing coating preparation includes a core material and a coating, and the coating solution for preparing the coating includes, by weight: 8-10 parts of soybean lecithin oil; 2-3 parts of reducing sugar; 18-20 parts of fat; the coating solution is prepared as follows: 8-10 parts of soybean lecithin oil are heated and melted at a high temperature of 60-90°C, 2-3 parts of reducing sugar are added to a molten reaction pot and stirred until melted, and the material is kept warm at 50°C after the reaction turns brown; another 18-20 parts of fat are heated and melted, the material obtained in the previous order is mixed with it, and stirred evenly to form a mixed solution, which is the coating solution. The present invention utilizes the Maillard reaction between lecithin and reducing sugar to produce a product with excellent rumen-passing and intestinal release effects, and the obtained product has high purity, good quality, and high small intestinal release rate, improves the vitality of immune cells, and enhances disease resistance and anti-stress capabilities.

Description

Technical Field

[0001] The invention relates to the technical field of animal feeding, in particular to feed additives, and in particular to a rumen bypass coating preparation for ruminants and a preparation method and application thereof. Background Art

[0002] In the livestock industry, people are paying more and more attention to high-quality meat, eggs and milk, so researchers have explored and developed many nutrients that can improve animal production performance, such as enzyme preparations, vitamin preparations, amino acids, etc. Monogastric animals can digest and absorb these substances directly, while ruminants usually need to process nutrients through the rumen due to the particularity of rumen digestion.

[0003] At present, the most commonly used rumen bypass technologies include physical treatment, chemical treatment, coating, microencapsulation and other technologies. Fluidized bed spray coating is often used in the coating of nutrients, and polypropylene resin IV is often used as the coating material for rumen bypass materials, which has a better effect. However, for some nutrients that are sensitive to environmental conditions, such as enzyme preparations, the acidic conditions released in the abomasum will inactivate the enzymes, for example:

[0004] Chinese invention patent application 201910501812.7 discloses a rumen-protected amylase and its preparation method, using polypropylene resin IV and chitosan as coating materials. The feed amylase described therein may be inactivated under the acidic conditions of the abomasum; and hydrogenated vegetable oils, etc., although released in the small intestine under the degradation of small intestinal pancreatic enzymes, have poor film-forming properties and have a certain disruptive effect on the rumen microbial flora. In addition, in the investigation and testing of materials, it was found that although hydrogenated vegetable oil has a high rumen-protected rate, because it is a saturated fatty acid, it is less degraded by rumen microorganisms, but the release rate in the small intestine is low.

[0005] Chinese invention patent application 202210791385.2 discloses a rumen bypass preparation, a preparation method and an application thereof. In this method, the ratio of polypropylene resin to anhydrous ethanol is 1:1, which can greatly reduce the amount of ethanol used. However, when ethanol melt is added to high-temperature molten fat, a large number of lumps will appear and they will not dissolve.

[0006] Chinese invention patent application 202011434613.8 discloses a rumen bypass product and a production method thereof, which utilizes white carbon black to adsorb palm oil to prepare a rumen bypass product. Although the small intestine release rate is stable, it is not high, and the product specific gravity is between 0.9-1.0, which is lower than the rumen fluid. The product suspended in the rumen fluid is very easy to induce rumination and be re-chewed by the mouth, causing damage to the product.

[0007] Chinese invention patent application 201910441320.3 discloses a ruminant bypass preparation and a preparation method and device thereof, wherein coating dry powder materials such as cellulose ester, cellulose ether, poly(meth)acrylate, acrylate and other polymer materials may affect rumen fermentation, and the small specific gravity of the coating material may lead to incomplete coating.

[0008] In summary, even if the product's rumen passing rate is increased, the inability to release biological activity is a huge loss for the product, and increasing the dosage corresponds to an increase in production costs. Therefore, it is urgent to find a way to improve the intestinal release rate of oil while increasing the rumen passing rate. Summary of the invention

[0009] In view of the deficiencies in the prior art, the present invention provides a rumen-free coated preparation and a preparation method and application thereof, so as to solve one or more technical problems in the prior art.

[0010] In order to achieve the above object, the technical solution adopted by the present invention is as follows:

[0011] The first aspect of the present invention provides a ruminant rumen bypass coating preparation, comprising a core material and a coating, wherein the coating solution for preparing the coating comprises, by weight:

[0012] Soybean lecithin oil: 8-10 parts;

[0013] Reducing sugar: 2-3 parts;

[0014] Oil: 18-20 parts;

[0015] The coating solution is prepared as follows:

[0016] Take 8-10 parts of soybean lecithin oil and heat it to melt at 60-90℃, add 2-3 parts of reducing sugar into the melting reaction pot and stir until it melts, and keep it at 50℃ after the material reacts and turns brown;

[0017] Take another 18-20 parts of oil and fat, heat and melt them, mix them with the material obtained in the previous step, and stir them evenly to form a mixed solution, which is the coating solution.

[0018] Preferably, the soybean lecithin oil is a solid paste, mainly containing lecithin, cephalin, inositol phospholipids, phosphatidylserine, and phosphatidic acid.

[0019] Preferably, the reducing sugar is one or more of glucose, fructose, galactose, lactose and xylose.

[0020] Preferably, the oil is a fatty acid having greater than or equal to 14 carbon atoms.

[0021] Preferably, the oil is one or more of stearic acid, tristearin, tripalmitin, coconut oil, hydrogenated palm oil, soybean wax and carnauba wax, hydrogenated soybean oil, eicosanoic acid, vaccenic acid, myristic acid, lauric acid, and palmitic acid.

[0022] The second aspect of the present invention provides a method for preparing the rumen-bypass coated preparation, comprising:

[0023] 1) Add anhydrous ethanol and talcum powder to the active ingredient powder in the core material, extrude the mixed material through a screen, and round the particles;

[0024] 2) Dry the spheronized particles, cool them to room temperature, and select particles with a mesh size of 20-30 as core materials;

[0025] 3) The dry matter mass of the core material and the coating liquid is fed according to (5-7):(3-5);

[0026] 4) Use a multifunctional fluidized bed granulator to spray the coating on the bottom. After spraying, cool the coating material to room temperature and sieve to obtain the rumen-passed coating preparation.

[0027] Preferably, after spraying the packaging material, the mixture is cooled to room temperature and passed through a 15-25 mesh sieve to obtain a rumen-coated preparation.

[0028] The third aspect of the present invention provides an apparatus for implementing the preparation method, comprising: a coating device which is a multifunctional fluidized bed granulation coating machine, wherein the material recovery rate is >99%, the moisture content of the finished product is <0.5%, and the fluidization and air flow conveying are combined through bottom spraying operation, and the same direction liquid spraying is adopted, and the nozzle is buried in the material, the probability of the particles being fluidized and passing through the spray mist particles is high, and the particles are highly dense in the liquid spraying area.

[0029] A fourth aspect of the present invention provides a use of the rumen bypass coating preparation in preparing a feed additive for ruminants.

[0030] A fifth aspect of the present invention provides a use of the rumen bypass coating preparation in the preparation of medicines for ruminants.

[0031] The beneficial effects of the present invention compared with the prior art are: the rumen-pass coating preparation provided by the present invention and its preparation method and application, the raw materials for preparing the coating are soybean lecithin oil, reducing sugar, and fat, and the obtained product has high purity, good quality, and high small intestine release rate. Specifically, it can at least bring the following beneficial effects:

[0032] (1) The rumen-pass coating preparation provided by the present invention comprises soybean lecithin and phospholipids in soybean lecithin oil, and utilizes the Maillard reaction between phosphatidylethanolamine contained in lecithin and reducing sugar to generate Maillard reaction products, which are mixed with oil and fat for spraying, thereby promoting the degradation of fatty acids in oil and fat, especially hydrogenated vegetable oil, in the small intestine while ensuring a high rumen-passing rate.

[0033] (2) The rumen-permeable coating preparation provided by the present invention contains lecithin in soybean lecithin oil. Lecithin can improve the digestibility of non-fat nutrients such as fat-soluble vitamins, control the movement and absorption of cholesterol, provide energy, and has antioxidant properties. It can improve the body's metabolic function, enhance the activity of immune cells, and enhance disease resistance and stress resistance.

[0034] It should be understood that the implementation of any embodiment of the present invention does not mean that multiple or all of the above-mentioned beneficial effects must be possessed or achieved at the same time. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] In order to more clearly illustrate the implementation methods of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for the implementation methods or the description of the prior art. Obviously, the drawings in the following description are only exemplary, and for ordinary technicians in this field, other implementation drawings can be derived from the provided drawings without creative work.

[0036] The structures, proportions, sizes, etc. illustrated in this specification are only used to match the contents disclosed in the specification so as to facilitate understanding and reading by persons familiar with the technology. They are not used to limit the conditions under which the present invention can be implemented, and therefore have no substantial technical significance. Any structural modification, change in proportion or adjustment in size shall still fall within the scope of the technical contents disclosed in the present invention without affecting the effects and purposes that can be achieved by the present invention.

[0037] Figure 1 A schematic diagram of a core material for preparing a rumen-bypass coating preparation provided in an embodiment of the present invention;

[0038] Figure 2 A schematic diagram of a product of a rumen-bypass coating preparation provided in an embodiment of the present invention;

[0039] Figure 3 This is a schematic diagram of a rumen-bypass coated formulation product in water provided by an embodiment of the present invention. DETAILED DESCRIPTION

[0040] In order to make the purpose, technical solution and advantages of the embodiments of the present invention more clearly understood, the embodiments of the present invention are further described in detail below in conjunction with the embodiments and drawings. Here, the illustrative embodiments of the present invention and their description are used to explain the present invention, but are not intended to limit the present invention.

[0041] It should be understood that the terms "include / comprise", "consist of..." or any other variations are intended to cover non-exclusive inclusion, so that a product, device, process or method that includes a series of elements includes not only those elements, but also may include other elements not explicitly listed when necessary, or also includes elements inherent to such product, device, process or method. In the absence of more restrictions, the elements defined by the sentence "include / comprise...", "consist of..." do not exclude the presence of other identical elements in the product, device, process or method that includes the elements.

[0042] Unless otherwise specified, the raw materials in the embodiments of the present invention can be purchased through commercial channels.

[0043] A ruminant rumen bypass coating preparation provided by an embodiment of the present invention comprises a core material and a coating, wherein the coating liquid is made of soybean lecithin oil, reducing sugar, and fat, and comprises, by weight: 8-10 parts of soybean lecithin oil; 2-3 parts of reducing sugar; 18-20 parts of fat; the coating liquid is prepared by the following method: 8-10 parts of soybean lecithin oil are heated and melted at a high temperature of 60-90°C, 2-3 parts of reducing sugar are added to a molten reaction pot and stirred until melted, and the material is kept warm at 50°C after the reaction turns brown; another 18-20 parts of fat are heated and melted, the material obtained in the previous order is mixed with the fat, and the mixed solution is stirred evenly to form a mixed solution, namely the coating liquid.

[0044] In some embodiments, soybean lecithin oil is a solid paste, which mainly contains lecithin, cephalin, inositol phospholipids, phosphatidylserine, and phosphatidic acid.

[0045] In some embodiments, the reducing sugar is one or more of glucose, fructose, galactose, lactose, xylose.

[0046] In some embodiments, the oil is a fatty acid having greater than or equal to 14 carbon atoms.

[0047] In some embodiments, the oil is one or more of stearic acid, tristearin, tripalmitin, coconut oil, hydrogenated palm oil, soybean wax and carnauba wax, hydrogenated soybean oil, eicosanoic acid, vaccenic acid, myristic acid, lauric acid, and palmitic acid.

[0048] The temperature of the oil can be heated to 50-80°C until it melts.

[0049] The technical principle of the rumen-pass coating preparation of the present invention is:

[0050] Oil is used in the ingredients to facilitate molding, otherwise the solution wrapped in soybean lecithin oil will stick together.

[0051] The Maillard reaction is a complex chemical reaction between amino compounds and carbonyl compounds (such as reducing sugars, lipids, aldehydes, ketones, etc.). The phosphatidylethanolamine contained in the phospholipids (soybean lecithin and egg phospholipids) in soybean lecithin oil can undergo the Maillard reaction with reducing sugars, and has high antioxidant and free radical scavenging capabilities. Therefore, the embodiment of the present invention utilizes the Maillard reaction between phospholipids and reducing sugars to produce a product with high rumen passing rate and excellent intestinal release effect.

[0052] In addition, the lecithin in soybean lecithin oil can improve the digestibility of non-fat nutrients such as fat-soluble vitamins, control the movement and absorption of cholesterol, provide energy, and has antioxidant properties. It can improve the body's metabolic function, enhance the vitality of immune cells, and enhance disease resistance and anti-stress capabilities.

[0053] Example 1

[0054] 1) Preparation of core coating material: Use enzyme feed medium-temperature α-amylase as the active ingredient in the core coating material. Amylase is in powder form. In order to facilitate coating, talcum powder is used as the core material auxiliary material. The specific operation is: take 85 parts of α-amylase powder, add 15 parts of talcum powder, add 10 parts of anhydrous ethanol and mix evenly. The mixed material is rotated and extruded through a 1.0mm sieve, spheronized by a spheronizer for 5 minutes, dried and cooled to room temperature, and 20-30 mesh particles are selected as core materials. The prepared core material (particles) are as follows Figure 1 shown.

[0055] 2) Take 18 parts of hydrogenated palm oil and heat it to 70°C to melt, heat and melt 9 parts of soybean lecithin oil and add 2 parts of xylose, mix the soybean lecithin oil and xylose at 80°C and stir until the material turns brown, and mix the material with the melted hydrogenated palm oil to obtain a coating solution.

[0056] 3) The weight of the core material and the coating liquid is 7:3. The bottom coating is carried out by fluidized coating machine. After spraying the coating material, it is cooled to room temperature and sieved to avoid sticking. The rumen-passed coating preparation is obtained by sieving with 15-25 mesh. The prepared rumen-passed coating preparation (granules) is as follows Figure 2 As shown, Figure 3 The figure shows the state of the prepared rumen-pass coating preparation (granules) immersed in water. After 16 hours of immersion, it is basically not dissolved, which directly indicates that the coating liquid of the present invention has a good coating effect on the core material, and indirectly indicates that the coating preparation is not easily degraded by rumen microorganisms in the rumen, which is particularly important for improving the rumen-pass rate.

[0057] Example 2

[0058] 1) Preparation of core material: same as in Example 1.

[0059] 2) Take 18 parts of hydrogenated palm oil and heat it to 70°C to melt, heat and melt 9 parts of soybean lecithin oil and add 2 parts of xylose, mix the soybean lecithin oil and xylose at 90°C and stir until the material turns brown, and mix the material with the melted hydrogenated palm oil to obtain a coating solution.

[0060] 3) The weight of the core material and the coating liquid is 6:4 for feeding. The fluidized coating machine is used for bottom spray coating. After spraying the coating material, it is cooled to room temperature and sieved to avoid adhesion. The 15-25 mesh sieve is used to obtain the rumen-passed coating preparation.

[0061] Example 3

[0062] 1) Preparation of core material: same as in Example 1.

[0063] 2) Take 18 parts of hydrogenated palm oil and heat it to 80°C to melt it, heat and melt 9 parts of soybean lecithin oil and add 2 parts of xylose, mix the soybean lecithin oil and xylose at 80°C and stir until the material turns brown, and mix the material with the melted hydrogenated palm oil to obtain a coating solution.

[0064] 3) The weight of the core material and the coating liquid is 5:5. The fluidized coating machine is used for bottom spray coating. After spraying the coating material, it is cooled to room temperature and sieved to avoid adhesion. The 15-25 mesh sieve is used to obtain the rumen-passed coating preparation.

[0065] Example 4

[0066] 1) Preparation of core material: same as in Example 1.

[0067] 2) Take 20 parts of soybean wax and heat it to 60°C to melt it, heat and melt 10 parts of soybean lecithin oil and add 3 parts of fructose, mix the soybean lecithin oil and fructose at 70°C and stir until the material turns brown, and mix the material with the melted soybean wax to obtain a coating solution.

[0068] 3) The weight of the core material and the coating liquid is 6:5 for feeding. The bottom coating is carried out by fluidized coating machine. After spraying the coating material, it is cooled to room temperature and sieved to avoid adhesion. The rumen-passed coating preparation is obtained by sieving with 15-25 mesh.

[0069] Example 5

[0070] 1) Preparation of core material: same as in Example 1.

[0071] 2) Take 20 parts of soybean wax and heat it to 50°C to melt it, heat and melt 10 parts of soybean lecithin oil and add 3 parts of fructose, mix the soybean lecithin oil and fructose at 60°C and stir until the material turns brown, and mix the material with the melted soybean wax to obtain a coating solution.

[0072] 3) The weight of the core material and the coating liquid is 7:4. The fluidized coating machine is used for bottom spray coating. After spraying the coating material, it is cooled to room temperature and sieved to avoid adhesion. The 15-25 mesh sieve is used to obtain the rumen-passed coating preparation.

[0073] Example 6

[0074] 1) Preparation of core material: same as in Example 1.

[0075] 2) Take 18 parts of stearic acid and heat it to 70°C to melt, heat and melt 9 parts of soybean lecithin oil, add 2 parts of xylose, mix the soybean lecithin oil and xylose at 60°C and stir until the material turns brown, and mix the material with the melted stearic acid to obtain a coating solution.

[0076] 3) The weight of the core material and the coating liquid is 6:4 for feeding. The fluidized coating machine is used for bottom spray coating. After spraying the coating material, it is cooled to room temperature and sieved to avoid adhesion. The 15-25 mesh sieve is used to obtain the rumen-passed coating preparation.

[0077] Comparative Example 1

[0078] 1) Preparation of core material: same as in Example 1.

[0079] 2) Take 20 parts of hydrogenated vegetable oil and heat it to 70°C to melt it to obtain a coating solution.

[0080] 3) The weight of the core material and the coating liquid is 6:4. The fluidized coating machine is used for bottom spray coating. After spraying the coating material, it is cooled to room temperature and sieved to avoid adhesion. After 15-25 days, the rumen-passed coating preparation is obtained by sieving.

[0081] Comparative Example 2

[0082] 1) Preparation of core material: same as in Example 1.

[0083] 2) Take 18 parts of hydrogenated palm oil and heat it to 70°C to melt it, heat and melt 9 parts of soybean lecithin oil and mix them evenly to obtain a coating solution.

[0084] 3) The weight of the core material and the coating liquid is 6:4 for feeding. The fluidized coating machine is used for bottom spray coating. After spraying the coating material, it is cooled to room temperature and sieved to avoid adhesion. The 15-25 mesh sieve is used to obtain the rumen-passed coating preparation.

[0085] Comparative Example 3

[0086] 1) Preparation of core material: same as in Example 1.

[0087] 2) Take 10 parts of hydrogenated palm oil and heat it to 70°C to melt, heat and melt 8 parts of soybean lecithin oil and add 2 parts of xylose, mix the soybean lecithin oil and xylose at 80°C and stir until the material turns brown, and mix the material with the melted hydrogenated palm oil to obtain a coating solution.

[0088] 3) The weight of the core material and the coating liquid is 6:4 for feeding. The fluidized coating machine is used for bottom spray coating. After spraying the coating material, it is cooled to room temperature and sieved to avoid adhesion. The 15-25 mesh sieve is used to obtain the rumen-passed coating preparation.

[0089] Comparative Example 4

[0090] 1) Preparation of core material: same as in Example 1.

[0091] 2) Take 25 parts of hydrogenated palm oil and heat it to 70°C to melt it, heat and melt 8 parts of soybean lecithin oil and add 2 parts of xylose, mix the soybean lecithin oil and xylose at 80°C and stir until the material turns brown, and mix the material with the melted hydrogenated palm oil to obtain a coating solution.

[0092] 3) The weight of the core material and the coating liquid is 6:4 for feeding. The fluidized coating machine is used for bottom spray coating. After spraying the coating material, it is cooled to room temperature and sieved to avoid adhesion. The 15-25 mesh sieve is used to obtain the rumen-passed coating preparation.

[0093] Comparative Example 5

[0094] 1) Preparation of core material: same as in Example 1.

[0095] 2) Take 18 parts of hydrogenated palm oil and heat it to 70°C to melt it, heat and melt 4 parts of soybean lecithin oil and add 2 parts of xylose, mix the soybean lecithin oil and xylose at 70°C and stir until the material turns brown, and mix the material with the melted hydrogenated palm oil to obtain a coating solution.

[0096] 3) The weight of the core material and the coating liquid is 5:4. The fluidized coating machine is used for bottom spray coating. After spraying the coating material, it is cooled to room temperature and sieved to avoid adhesion. The 15-25 mesh sieve is used to obtain the rumen-passed coating preparation.

[0097] Comparative Example 6

[0098] 1) Preparation of core material: same as in Example 1.

[0099] 2) Take 18 parts of hydrogenated palm oil and heat it to 70°C to melt it, heat and melt 16 parts of soybean lecithin oil and add 2 parts of xylose, mix the soybean lecithin oil and xylose at 70°C and stir until the material turns brown, and mix the material with the melted hydrogenated palm oil to obtain a coating solution.

[0100] 3) The weight of the core material and the coating liquid is 5:4. The fluidized coating machine is used for bottom spray coating. After spraying the coating material, it is cooled to room temperature and sieved to avoid adhesion. The 15-25 mesh sieve is used to obtain the rumen-passed coating preparation.

[0101] Comparative Example 7

[0102] 1) Preparation of core material: same as in Example 1.

[0103] 2) Take 18 parts of hydrogenated palm oil and heat it to 70°C to melt, heat and melt 8 parts of soybean lecithin oil and add 1 part of xylose, mix the soybean lecithin oil and xylose at 70°C and stir until the material turns brown, mix the material with the melted hydrogenated palm oil to obtain a coating solution.

[0104] 3) The weight of the core material and the coating liquid is 7:4. The fluidized coating machine is used for bottom spray coating. After spraying the coating material, it is cooled to room temperature and sieved to avoid adhesion. The 15-25 mesh sieve is used to obtain the rumen-passed coating preparation.

[0105] Comparative Example 8

[0106] 1) Preparation of core material: same as in Example 1.

[0107] 2) Take 18 parts of hydrogenated palm oil and heat it to 70°C to melt, heat and melt 8 parts of soybean lecithin oil and add 5 parts of xylose, mix the soybean lecithin oil and xylose at 70°C and stir until the material turns brown, and mix the material with the melted hydrogenated palm oil to obtain a coating solution.

[0108] 3) The weight of the core material and the coating liquid is 7:4. The fluidized coating machine is used for bottom spray coating. After spraying the coating material, it is cooled to room temperature and sieved to avoid adhesion. The 15-25 mesh sieve is used to obtain the rumen-passed coating preparation.

[0109] Comparative Example 9

[0110] 1) Preparation of core material: same as in Example 1.

[0111] 2) Take 18 parts of hydrogenated palm oil and heat it to 80°C to melt it, heat and melt 8 parts of soybean lecithin oil and add 2 parts of xylose, mix the soybean lecithin oil and xylose at 50°C and stir until the material turns brown, and mix the material with the melted hydrogenated palm oil to obtain a coating solution.

[0112] 3) The weight of the core material and the coating liquid is 7:4. The fluidized coating machine is used for bottom spray coating. After spraying the coating material, it is cooled to room temperature and sieved to avoid adhesion. The 15-25 mesh sieve is used to obtain the rumen-passed coating preparation.

[0113] Comparative Example 10

[0114] 1) Preparation of core material: same as in Example 1.

[0115] 2) Take 20 parts of hydrogenated palm oil and heat it to 70°C to melt it, heat and melt 8 parts of soybean lecithin oil and add 2 parts of xylose, mix the soybean lecithin oil and xylose at 100°C and stir until the material turns brown, and mix the material with the melted hydrogenated palm oil to obtain a coating solution.

[0116] 3) The weight of the core material and the coating liquid is 7:4. The fluidized coating machine is used for bottom spray coating. After spraying the coating material, it is cooled to room temperature and sieved to avoid adhesion. The 15-25 mesh sieve is used to obtain the rumen-passed coating preparation.

[0117] The results of the rumen bypass effect and intestinal release rate of each embodiment and comparative example are shown in Table 1 below.

[0118] Table 1 Results of rumen bypass effect and intestinal release rate

[0119]

[0120] By comparing the embodiments with Comparative Examples 1 and 2, it can be seen that Comparative Example 1 only added hydrogenated vegetable oil, and Comparative Example 2 did not use reducing sugar, indicating that the Maillard reaction could not occur without using soybean lecithin oil or reducing sugar, resulting in greatly reduced 16h rumen passing rate and 24h small intestine release rate, and poor rumen passing rate and intestinal release effects.

[0121] By comparing the embodiments with Comparative Examples 3 and 4, it can be seen that 10 parts and 25 parts of fat (hydrogenated palm oil) were used respectively. Low or high fat dosage will lead to material adhesion and poor intestinal release effect.

[0122] By comparing the embodiments with Comparative Examples 5 and 6, it can be seen that 4 parts and 10 parts of soybean lecithin oil were used respectively. Low or high dosage of soybean lecithin oil will lead to poor intestinal release effect and material adhesion.

[0123] By comparing the embodiments with Comparative Examples 7 and 8, it can be seen that 1 part and 5 parts of xylose were used respectively. Low or high amounts of xylose will result in the inability to form the Maillard reaction or an excessive reaction resulting in inability to release in the intestine.

[0124] By comparing the embodiments with Comparative Examples 9 and 10, it can be seen that the high-temperature melting temperatures of soybean lecithin oil and xylose are 50°C and 100°C, respectively. If the temperature of the reduction reaction is too high or too low, the Maillard reaction will not be moderate, resulting in the inability to form antioxidant beneficial factors or excessive formation of harmful substances.

[0125] Determination method:

[0126] Rumen passage rate was determined by semi-in vivo nylon bag method.

[0127] Nylon bags with a pore size of 40um and a size of 12cmx10cm are numbered in advance, washed and dried at 65°C for 48h, and then weighed. Use an analytical balance to weigh 5g of sample and put it into a nylon bag of known weight. Nylon bag placement: Use nylon rope to tie the nylon bags containing samples to different iron chains. Each iron chain can tie up to 12 nylon bags, and 3 nylon bags are used as parallel for each sample at each time point. According to the principle of "putting in and taking out at the same time", the iron chain tied with the nylon bag is placed into the rumen abdominal bag through the rumen fistula, and one end of the iron rod is tied to the fistula cover with a nylon rope. Each fistula cow is placed with an iron chain, and the nylon bags are taken out after fermentation in the body for the same time. Cleaning: Rinse the taken out nylon bag immediately under tap water until the water flowing out of the nylon bag is clear and bright. After cleaning, gently squeeze out the excess water with your hands. Drying: Dry the nylon bag at 45°C for 48 h, take it out, weigh it, and transfer it to a sealed bag for subsequent analysis.

[0128] Rumen degradation rate = (sample weight in bag – rumen weight after digestion at each time point) / sample weight in bag × 100%.

[0129] Abomasum small intestine digestion: Pepsin and pancreatic enzyme digestion tests were performed according to the modified in vitro three-step method recommended by Gargallo et al. The residue sample after 16 hours of digestion was prepared using the rumen stability test method. 0.5g of the residue after 16 hours of in vitro rumen degradation was weighed into a fiber bag (ANKOM-FN57), sealed and placed in a culture bottle, with a maximum of 30 fiber bags in each bottle. Three replicates were set up for each group, for a total of nine groups and 27 fiber bags. 2L of hydrochloric acid solution with a pH of 1.9 containing 1g / L pepsin (P-7000, Sigma) was added to the culture bottle, and the solution was preheated in advance. The culture bottle was placed in an in vitro simulated fermentation incubator (Daisy Ⅱ, Ankom, USA) and rotated at 39°C for 1 hour. Add 2L of preheated 0.5mol / L phosphate buffer containing 3g / L trypsin and 50mg / L thymol, and place the culture bottle in an in vitro simulated fermentation incubator (Daisy Ⅱ, Ankom, USA) and rotate and culture at 39℃ for 24h. Take out the fiber bag and wash it until the water is clear, and bake it in a 65℃ oven for 48h to constant weight.

[0130] Small intestine degradation rate = (sample weight before small intestine digestion – sample weight after small intestine digestion) / sample weight before digestion × 100%.

[0131] Industrial Applicability

[0132] The rumen-passing coating preparation provided by the embodiment of the present invention is mainly used in the field of agricultural animal husbandry, and can be prepared as a feed additive for ruminants, as well as a medicine for ruminants. It is composed of rumen-passing nutrients, soybean lecithin oil, fats, reducing sugars, adhesives, and dispersants. After the nutrients are granulated by a granulating shot blasting and spheronization device, a fluidized bed granulating coating machine is used to coat them in a bottom spraying manner. The Maillard reaction is used to generate the Maillard reaction product, and the Maillard product is mixed and sprayed with the fat, which promotes degradation in the small intestine and improves the small intestine release rate while ensuring a high rumen-passing rate.

[0133] The embodiments of the present invention have been described above, and the above description is exemplary, not exhaustive, and is not limited to the disclosed embodiments. Many modifications and variations will be apparent to those of ordinary skill in the art without departing from the scope and spirit of the described embodiments. The selection of terms used herein is intended to best explain the principles of the embodiments, practical applications, or technical improvements in the market, or to enable other persons of ordinary skill in the art to understand the embodiments disclosed herein.

Claims

1. A ruminant rumen-free coating preparation, comprising a core material and a coating, characterized in that: The coating solution for preparing the coating comprises, by weight: Soybean lecithin oil: 8-10 parts; Reducing sugar: 2-3 parts; Oil: 18-20 parts; The coating solution is prepared as follows: Take 8-10 parts of soybean lecithin oil and heat it to melt at 60-90℃, add 2-3 parts of reducing sugar into the melting reaction pot and stir until it melts, and keep it at 50℃ after the material reacts and turns brown; Take another 18-20 parts of oil and fat, heat and melt them, mix them with the materials obtained in the previous step, and stir them evenly to form a mixed solution, which is the coating solution.

2. The rumen bypass coating preparation according to claim 1, characterized in that: The soybean lecithin oil is a solid paste, which mainly contains lecithin, cephalin, inositol phospholipids, phosphatidylserine and phosphatidic acid.

3. The rumen bypass coating preparation according to claim 1, characterized in that: The reducing sugar is one or more of glucose, fructose, galactose, lactose and xylose.

4. The rumen bypass coating preparation according to claim 1, characterized in that: The oil is a fatty acid having greater than or equal to 14 carbon atoms.

5. The rumen bypass coating preparation according to claim 4, characterized in that: The oil is one or more of stearic acid, tristearin, tripalmitin, coconut oil, hydrogenated palm oil, soybean wax and carnauba wax, hydrogenated soybean oil, eicosanoic acid, vaccenic acid, myristic acid, lauric acid, and palmitic acid.

6. A method for preparing the rumen-bypass coated preparation according to any one of claims 1 to 5, characterized in that: include: 1) Add anhydrous ethanol and talcum powder to the active ingredient powder in the core material, extrude the mixed material through a screen, and round the particles; 2) Dry the spheronized particles, cool them to room temperature, and select particles with a mesh size of 20-30 as core materials; 3) The dry matter mass of the core material and the coating liquid is fed according to (5-7):(3-5); 4) Use a multifunctional fluidized bed granulator to spray the coating on the bottom. After spraying the coating material, cool it to room temperature and sieve it to obtain the rumen-passed coating preparation.

7. The preparation method according to claim 6, characterized in that: After spraying the packaging material, the mixture is cooled to room temperature and passed through a 15-25 mesh sieve to obtain a rumen-passed coating preparation.

8. Use of the rumen bypass coating formulation according to any one of claims 1 to 5 in the preparation of a feed additive for ruminants.

9. Use of the rumen bypass coating formulation according to any one of claims 1 to 5 in the preparation of a medicament for ruminants.

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