A firming and anti-wrinkle composition, a facial cream and a preparation method thereof

Abstract

The present invention discloses a firming and anti-wrinkle composition, a cream and a preparation method thereof, specifically belonging to the field of toilet preparations; the firming and anti-wrinkle cream contains components such as a firming and anti-wrinkle composition, a composite polypeptide, recombinant collagen, a skin conditioner, etc. The firming and anti-wrinkle composition contains extracts of Leontopodium alpinum flower / leaf, peach resin extract, gentian root extract, and nasturtium extract. The composite polypeptide is at least two of palmitoyl tripeptide-1, palmitoyl tetrapeptide-7, acetyl hexapeptide-8, and palmitoyl hexapeptide-12. The recombinant collagen is at least one of recombinant type XVII collagen, recombinant type I collagen, recombinant type IV collagen, recombinant type V collagen, and recombinant type VII collagen. The firming and anti-wrinkle cream provided by the present invention can effectively increase the elasticity value of the skin, can significantly improve the firmness of the skin, and has excellent anti-wrinkle and anti-aging effects.

Description

Technical Field

[0001] The present invention belongs to the field of toilet preparations, and particularly relates to a firming and anti-wrinkle composition, a cream and a preparation method thereof. Background Art

[0002] With the increasing demands of people for skin health and beauty, firming and anti-wrinkle have become an important research direction in the fields of cosmetics and skin care products. In existing firming and anti-wrinkle products, the combined use of various ingredients has become a trend. These ingredients usually include plant extracts, polypeptides, vitamins, etc., which achieve the effects of firming and anti-wrinkle through different mechanisms of action, such as promoting collagen production, inhibiting free radical production, improving skin microcirculation, etc. However, there are still many deficiencies in these compositions in terms of formulation design, ingredient selection, mechanism of action, etc., such as unclear interactions between ingredients, poor stability, and insignificant effects.

[0003] Although certain progress has been made in existing firming and anti-wrinkle products, they still face the following problems and challenges: First, the interactions between ingredients are not clear. Traditional compositions often lack in-depth research on the interactions between ingredients and it is difficult to achieve the best effects. Second, the stability is poor. Some ingredients are prone to chemical reactions or degradation under specific conditions, resulting in poor product stability and affecting the use effect. For example, Chinese Patent CN117205113A discloses a firming and anti-wrinkle composition, including components: extract of tuber melanosporum, hydroxypropyltetrahydropyrantriol, cananga odorata oil-3, eucalyptus oil, bay leaf oil and an emulsifier. Since the composition contains essential oil components, it is prone to decomposition and volatilization under specific conditions, affecting the use. Third, the effects are not significant. Due to the limitations of ingredient selection and mechanism of action, existing products often fail to achieve significant firming and anti-wrinkle effects and cannot meet the needs of consumers. Summary of the Invention

[0004] Aiming at the deficiencies of the existing technology, the purpose of the present invention is to provide a firming and anti-wrinkle composition containing extracts of leontopodium alpinum flower / leaf, peach resin extract, gentiana root extract, and nasturtium officinale extract, as well as a cream containing this composition, composite polypeptide, and recombinant collagen. The ingredients are scientifically proportioned to achieve a synergistic effect and significantly improve the firming and anti-wrinkle effect of the overall formula.

[0005] To achieve the above purpose, the present invention discloses the following technical solutions:

[0006] In the first aspect, the present invention provides a firming and anti-wrinkle composition. By mass, the composition contains the following components:

[0007] Extract of leontopodium alpinum flower / leaf 2 - 5 parts;

[0008] Peach resin extract 1 - 6 parts;

[0009] 0.5 - 0.7 parts of gentian root extract;

[0010] 1 - 4 parts of nasturtium extract.

[0011] Preferably, the preparation method of the nasturtium extract comprises the following steps:

[0012] S1 - 1. Take nasturtium, crush and sieve it to obtain nasturtium powder;

[0013] S1 - 2. Add deionized water to the nasturtium powder, perform ultrasonic treatment for 20 - 30 min, and filter to obtain filtrate A and residue A for standby;

[0014] S1 - 3. Add an ethanol aqueous solution with ≥95 v / v% to residue A, and perform reflux extraction, then filter to obtain filtrate B;

[0015] S1 - 4. After combining filtrate A and filtrate B, perform concentration and drying to obtain nasturtium extract.

[0016] More preferably, the mesh number of sieving in step S1 - 1 is 60 meshes.

[0017] More preferably, in step S1 - 2, the mass ratio of nasturtium powder to deionized water is 1:10, and the ultrasonic frequency is 28 kHz.

[0018] More preferably, in step S1 - 3, the addition amount of the ethanol aqueous solution is 5 times the mass of the residue, the temperature of reflux extraction is controlled at 85°C, the number of reflux times is 3 times, and each reflux time is 0.7 h.

[0019] In a second aspect, the present invention provides the use of the firming and anti - wrinkle composition described in the first aspect in the preparation of lotion, essence, gel, emulsion and / or cream having firming and anti - wrinkle effects.

[0020] In a third aspect, the present invention provides a cream having firming and anti - wrinkle effects, wherein the cream contains the firming and anti - wrinkle composition described in the first aspect, and the addition amount of the firming and anti - wrinkle composition in the cream is ≤3 wt%.

[0021] Preferably, the cream further contains composite polypeptide, recombinant collagen, skin conditioner, thickener, emulsifier, emollient, preservative, chelating agent, moisturizer, solvent.

[0022] More preferably, the composite polypeptide is at least two of palmitoyl tripeptide - 1, palmitoyl tetrapeptide - 7, acetyl hexapeptide - 8, dipeptide diaminobutyryl benzylamide diacetate, carnosine, nonapeptide - 1;

[0023] The recombinant collagen is at least one of recombinant type XVII collagen, recombinant type I collagen, recombinant type IV collagen, recombinant type V collagen, and recombinant type VII collagen;

[0024] The skin conditioner is at least one of polydeoxyribonucleotide, niacinamide, octanoyl hydroxamic acid, and 3-O-ethyl ascorbic acid;

[0025] The thickener is at least one of sodium polyacrylate, acrylate / C10-30 alkyl acrylate cross-linked polymer, xanthan gum, carbomer, and polyacrylamide;

[0026] The emulsifier is at least one of PEG-20 methyl glucoside sesquistearate, glyceryl stearate, sorbitan stearate, PEG-100 stearate, and cetearyl glucoside;

[0027] The emollient is at least one of dicaprylyl carbonate, cetearyl alcohol, hydrogenated polyisobutene phenyl trimethylsiloxane, tocopheryl acetate, C13-14 isoparaffin, jojoba seed oil, squalane, caprylic / capric triglyceride, ethylhexyl palmitate, shea butter, polydimethylsiloxane, lavender oil, grapefruit peel oil, ylang-ylang oil, lemon peel oil, and rosa damascena flower oil;

[0028] The preservative is at least one of p-hydroxyacetophenone, methylparaben, octanoyl hydroxamic acid, glycerol caprylate, 1,2-pentanediol, phenoxyethanol, ethylhexylglycerin, and octanediol;

[0029] The chelating agent is at least one of disodium EDTA and tetrahydroxypropyl ethylenediamine;

[0030] The humectant is at least one of β-glucan, glycerol, sodium polyglutamate, tremella fuciformis fruiting body extract, sodium hyaluronate, allantoin, butanediol, propylene glycol, and 1,2-hexanediol;

[0031] The solvent is deionized water.

[0032] Fourthly, the present invention provides a preparation method of the cream with the effect of firming and anti-wrinkle described in the third aspect. The preparation method includes the following steps:

[0033] Mix the firming and anti-wrinkle composition, composite polypeptide, recombinant collagen, skin conditioner, thickener, emulsifier, emollient, preservative, chelating agent, humectant, and solvent evenly to obtain the cream with the effect of firming and anti-wrinkle.

[0034] In the present invention:

[0035] The extract of Leontopodium alpinum flower / leaf contains various active ingredients, including volatile oils, flavonoids, phenylpropanoids, isobenzofuranones, and terpenoid compounds, etc. These ingredients endow it with multiple effects such as antioxidant, anti-inflammatory, antibacterial and anti-inflammatory, protecting the skin barrier, and brightening the skin. It can scavenge free radicals, reduce the damage of ultraviolet rays to the skin, prevent photoaging, and at the same time promote collagen production, improving skin elasticity and firmness.

[0036] The extract of peach resin is rich in polysaccharide substances, including arabinose, galactose, xylose, mannose, etc. In addition, the extract of peach resin also contains natural antioxidant active substances such as phenols and flavonoids. The extract of peach resin can maintain the water balance inside and outside skin cells, thus achieving the moisturizing effect; it has an anti-inflammatory effect, which can reduce skin inflammation and allergic reactions; it has the effect of repairing and strengthening the skin barrier and promoting wound healing; it can promote the proliferation of fibroblasts and the synthesis of type I collagen, reduce the activity of β-galactosidase, and has the effect of anti-wrinkle and firming the skin; the phenols and flavonoids it contains have antioxidant activity, can scavenge free radicals, and reduce skin damage.

[0037] The extract of gentian root is rich in chemical components such as iridoids, triterpenoids, ketones, and flavonoids. It can reduce skin inflammation, resist free radical damage, inhibit the growth of microorganisms, and promote the renewal and repair of skin cells, thereby improving the skin barrier function. In addition, the extract of gentian root can also regulate sebum secretion and improve the state of oily and rough skin, and it is a multi-functional skin care ingredient.

[0038] The extract of Tropaeolum majus contains rich active ingredients such as flavonoid compounds, alkaloids, and polysaccharides. These ingredients endow it with multiple skin care effects. In cosmetics, the extract of Tropaeolum majus mainly plays antibacterial, anti-inflammatory, and antioxidant roles. It can effectively inhibit the bacteria on the skin surface, reduce inflammation and redness, and at the same time neutralize free radicals, protect the skin from oxidative damage, and delay skin aging.

[0039] The research of the present invention finds that the combined use of the extract of Leontopodium alpinum flower / leaf, the extract of peach resin, the extract of gentian root, and the extract of Tropaeolum majus can act on the skin more effectively to achieve the effect of firming and anti-wrinkle, and slow down skin aging to a certain extent.

[0040] The beneficial effects of the present invention:

[0041] 1. The combined use of the extract of Leontopodium alpinum flower / leaf, the extract of peach resin, the extract of gentian root, and the extract of Tropaeolum majus in the present invention can effectively promote collagen production, improve skin elasticity, and make the skin achieve the effect of firming and anti-wrinkle;

[0042] 2. The firming and anti-wrinkle cream provided by the present invention can effectively improve the elasticity value of the skin, significantly improve the firmness of the skin, and has excellent anti-wrinkle and anti-aging effects. Detailed implementation manners

[0043] To better illustrate the purpose, technical solution and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments. Those skilled in the art should understand that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0044] Unless otherwise specified, the test methods used in the examples and comparative examples are all conventional methods; the materials, reagents, etc. used, unless otherwise specified, can all be obtained from commercial channels; the percentages mentioned in the examples and comparative examples, unless otherwise specified, are all mass percentages.

[0045] In the present invention:

[0046] The extract of Leontopodium alpinum flower / leaf is the extract of Leontopodium alpinum flower / leaf, with the INCI name of LEONTOPODIUM ALPINUM FLOWER / LEAF EXTRACT, purchased from Shanghai Jiayu Biotechnology Co., Ltd.;

[0047] The extract of Prunus persica resin is the extract of Prunus persica resin, with the INCI name of PRUNUS PERSICA(PEACH) RESIN EXTRACT, purchased from Shanghai Jiayu Biotechnology Co., Ltd.;

[0048] The extract of Gentiana scabra root is the extract of Gentiana scabra root, with the INCI name of GENTIANA SCABRAROOT EXTRACT, purchased from Shanghai Jiayu Biotechnology Co., Ltd.;

[0049] Tropaeolum majus is a plant of the genus Tropaeolum in the family Tropaeolaceae, and its Latin scientific name is Tropaeolum majus L. , purchased from Guangzhou Qingping Chinese herbal medicine market, and the variety has been identified;

[0050] All other raw materials are commercially available.

[0051] Preparation of Tropaeolum majus extract

[0052] Step S1-1. Take the dried Tropaeolum majus and crush it with a high-speed crusher, and pass through a 60-mesh sieve to obtain Tropaeolum majus powder;

[0053] Step S1-2. Add 10 times the mass of deionized water to the Tropaeolum majus powder, and perform ultrasonic treatment at 28 kHz for 20 min, and filter to obtain filtrate A and filter residue A for standby;

[0054] Step S1-3. Add 5 times the mass of 95 v / v% ethanol aqueous solution to the filter residue A, and perform reflux extraction. The temperature of the reflux extraction is 85 °C, the number of refluxes is 3 times, and each reflux is for 0.7 h. Filter to obtain filtrate B;

[0055] Step S1-4. After combining filtrate A and filtrate B, perform concentration and vacuum drying to obtain the Trollius chinensis extract.

[0056] Preparation of the firming and anti-wrinkle composition

[0057] Compound and mix the raw materials according to the formulation amounts in Table 1 to obtain the firming and anti-wrinkle compositions 1-9.

[0058] Table 1 Raw materials and their mass ratios in the firming and anti-wrinkle composition

[0059] Composition 1 Composition 2 Composition 3 Composition 4 Composition 5 Composition 6 Composition 7 Composition 8 Composition 9 Leontopodium alpinum flower / leaf extract 2 3 5 - 3 3 3 3 1 Prunus persica resin extract 1 3 6 3 - 3 3 3 1 Gentiana scabra root extract 0.5 0.6 0.7 0.6 0.6 - 0.6 - 1 Tropaeolum majus extract 1 2.5 4 2.5 2.5 2.5 - - 1

[0060] Note: “-” in the table indicates no addition.

[0061] To evaluate the firming and anti-wrinkle performance of the firming and anti-wrinkle composition, respectively take the firming and anti-wrinkle compositions 1-9 prepared above and dilute them with deionized water into the test sample solutions 1-9 with a concentration of 3 wt% for performance testing.

[0062] Performance testing

[0063] Anti-wrinkle efficacy test on zebrafish

[0064] Method source: “Standard Operating Procedure for the Evaluation of Anti-wrinkle Efficacy of Zebrafish”

[0065] System and sample size: Test system: Wild-type AB strain zebrafish.

[0066] Zebrafish age: 4 days post-fertilization (4 dpf).

[0067] Sample size for each group of experiments: 30 tails (three biological replicates, N = 3).

[0068] Test sample: Test sample solutions 1-9.

[0069] Principle and method: The growth, repair, nutrition, elasticity and tension of the skin are all related to collagen. Its loss will reduce the smoothness of the skin and cause wrinkles. In tetrapods, type I collagen is a trimer mainly composed of two α1 chains and one α2 chain, which are encoded by the col1a1a and col1a2 genes respectively, and perform collagen-related biological functions in connective tissues and bones. There are three type I collagen genes in zebrafish, which encode col1a1a, col1a1b and col1a2 of the α1(I), α2(I) and α3(I) chains respectively. Therefore, by detecting the relative expression levels of the col1a1a or / and col1a1b or / and col1a2 genes, it can be indicated whether the test sample has anti-wrinkle efficacy.

[0070] In this experiment, the anti-wrinkle effects of compositions 1-9 were detected by detecting the relative expression level of the col1a1a gene. The test results are shown in Table 2.

[0071] Zebrafish firming efficacy experiment

[0072] Method source: "Standard Operating Procedure for Zebrafish Firming Efficacy Evaluation Experiment"

[0073] System and sample size: Test system: Wild-type AB strain zebrafish.

[0074] Zebrafish age: 4 days post-fertilization (4 dpf).

[0075] Sample size for each group of experiments: 30 tails (three biological replicates, N = 3).

[0076] Test sample: Test sample solutions 1-9.

[0077] Principle and method: Elastin is the main component of elastic fibers in skin tissues, which can provide structural support for the skin and protect the skin from aging and sagging. Elastin is composed of two types of short peptide segments arranged alternately. Eln1 and eln2 are responsible for encoding different peptide segments of elastin. The two genes jointly regulate the expression level of elastin, making the skin firm and elastic. Zebrafish have elastin (eln1, eln2) genes similar to those of humans. Therefore, by detecting the relative expression levels of the eln1 or / and eln2 genes, it can be indicated whether the test sample has firming efficacy.

[0078] In this experiment, the firming effects of compositions 1-9 were detected by detecting the relative expression level of the eln1 gene. The test results are shown in Table 2.

[0079] Table 2 Firming and anti-wrinkle test results of the compositions

[0080] Group Relative expression level of col1a1a gene Relative expression level of eln1 gene Composition 1 3.04 3.15 Composition 2 3.11 3.42 Composition 3 2.97 3.1 Composition 4 1.43 1.69 Composition 5 1.56 2.04 Composition 6 1.23 1.52 Composition 7 1.35 1.85 Composition 8 0.89 1.19 Composition 9 2.25 2.68

[0081] Result analysis:

[0082] 1. The firming and anti-wrinkle composition provided by the present invention has an obvious promoting effect on the expression of collagen and elastin in zebrafish;

[0083] 2. From the performance test results of Compositions 4-7, it can be seen that when any one of the components is missing in the firming and anti-wrinkle composition provided by the present invention, it has a greater impact on the firming and anti-wrinkle performance of the composition, proving the key role of Leontopodium alpinum flower / leaf extract, peach resin extract, gentian root extract, and nasturtium officinale extract in the formula; from the performance results of Composition 9, it can be seen that when the ratio in the composition is not within a specific range, the performance of the composition will decline.

[0084] Preparation of the firming and anti-wrinkle cream:

[0085] Weigh each raw material according to the formula amount in Table 3;

[0086] Step S2-1, add part of deionized water to the batching pot and heat it to 85°C, then sequentially add sodium polyacrylate, acrylate / C10-30 alkyl acrylate cross-linked polymer, PEG-10 polydimethylsiloxane, squalane, polydimethylsiloxane, glycerin, disodium EDTA, allantoin, and part of butanediol, and homogenize and disperse at a rotation speed of 1000 r / min for 30 min, and keep warm for 30 min to obtain mixture C;

[0087] Step S2-2, heat the remaining butanediol to 60°C, add p-hydroxyacetophenone and 1,2-hexanediol, and dissolve completely to obtain mixture D;

[0088] Step S2-3, cool mixture C to 45°C, and then add mixture D to it, and stir evenly to obtain mixture CD;

[0089] Step S2-4, cool mixture CD to 40°C, add each component in A, B, and E and the remaining deionized water, and stir evenly and discharge at 200 r / min to obtain the firming and anti-wrinkle cream.

[0090] Table 3 Mass percentages of raw materials for the firming and anti-wrinkle cream

[0091]

[0092] Allergy test

[0093] The above Examples 1-3 were subjected to an allergy test by referring to the human skin patch test in the "2015 Cosmetics Safety and Technology Specifications".

[0094] The method of the skin occlusive patch test is as follows: Select 90 persons aged 18-60 years old, and select an area not exceeding 50 mm 2, A qualified patch test device with a depth of approximately 1 mm was used. 0.020 mL of the finished product prepared in the above-mentioned example was placed in the patch test chamber. The control hole was a blank control (without placing any substance). The patch test device with the added finished product was applied to the flexor side of the forearm of the subject with a low-sensitization tape, and gently pressed with the palm to make it evenly adhere to the skin for 24 h. The skin reactions were observed respectively at 30 min (after the indentation disappeared), 24 h, and 48 h after removing the finished product, and the observation results were recorded.

[0095] All subjects showed a reaction level of "-", a grade 0 reaction; that is, a negative reaction, without any allergic phenomenon.

[0096] Human efficacy experiment

[0097] 1. Subject screening

[0098] There were 15 subjects in total, aged 25 - 30 years old, female, with small differences in skin conditions. On average, there were 5 people in each group, and each group corresponded to testing Examples 1 - 3.

[0099] 2. Rules for using the test article

[0100] The subjects used it once in the morning and once in the evening every day. The essence was evenly applied to the face until it was completely absorbed. During the test period, the subjects needed to stop using other products of the same type. For 8 consecutive weeks, and there was a follow-up visit at 0, 4, and 8 weeks respectively, for a total of 3 times.

[0101] On the day of the test, the subjects needed to clean their faces with the cleansing product provided by the laboratory and sit still for 30 min. Subsequently, the researcher tested the skin on both cheeks respectively. The whole test was carried out in a constant temperature environment with a temperature of (21.0 ± 1.0) °C and a relative humidity of 50% ± 10%.

[0102] 3. Test method

[0103] The elastic parameter R2 of the cheek was measured using a 2 mm probe of Cutometer® MPA 580. R2 = Ua / Uf, which represents the ratio of the skin's resilience without negative pressure to the maximum stretch with negative pressure, and is considered to be the parameter value closest to the true state of skin elasticity. The closer the ratio is to 1, the better the skin elasticity is characterized. The average value was taken from 5 measurement values, and the results are shown in Table 4.

[0104] 4. Test results

[0105] Table 4 Skin elasticity R2 values

[0106]

[0107] 5. Result analysis

[0108] According to the results of Examples 1-3, it can be seen that the greater the concentration of the composition, the greater the change value of the skin elasticity R2 value, indicating that the composition has excellent firming and anti-wrinkle effects and has a certain concentration dependence; the firming and anti-wrinkle cream provided by the present invention can effectively increase the elasticity value of the skin, that is to say, the cream can significantly improve the firmness of the skin and has a significant anti-wrinkle effect.

[0109] Finally, it should be noted that the above examples and comparative examples are only used to illustrate the technical solutions of the present invention rather than to limit the protection scope of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that the technical solutions of the present invention can be modified or equivalently replaced without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A firming and anti-wrinkle composition, characterized in that, By mass parts, the composition consists of the following components: Leontopodium alpinum flower / leaf extract 2 - 5 parts; Peach resin extract 1 - 6 parts; Gentiana root extract 0.5 - 0.7 parts; Tropaeolum majus extract 1 - 4 parts; The preparation method of the Tropaeolum majus extract comprises the following steps: S1 - 1. Take Tropaeolum majus, crush and sieve it to obtain Tropaeolum majus powder; S1 - 2. Add deionized water to the Tropaeolum majus powder, ultrasonically treat for 20 - 30 min, and filter to obtain filtrate A and residue A for standby; S1 - 3. Add an ethanol aqueous solution with ≥95 v / v% to the residue A, and carry out reflux extraction, then filter to obtain filtrate B; S1 - 4. After combining filtrate A and filtrate B, carry out concentration and drying to obtain Tropaeolum majus extract; The mesh number of sieving in step S1 - 1 is 60 meshes; In step S1 - 2, the mass ratio of Tropaeolum majus powder to deionized water is 1:10, and the ultrasonic frequency is 28 kHz; In step S1 - 3, the addition amount of the ethanol aqueous solution is 5 times the mass of the residue, the reflux extraction temperature is controlled at 85 °C, the number of reflux times is 3 times, and each reflux time is 0.7 h.

2. Use of the firming and anti - wrinkle composition according to claim 1 in the preparation of lotion, essence, gel, emulsion and / or cream having firming and anti - wrinkle effects.

3. A cream with a firming and anti-wrinkle effect, characterized in that, A cream contains the firming and anti - wrinkle composition according to claim 1, and the addition amount of the firming and anti - wrinkle composition in the cream is 1 - 3 wt%.

4. The facial cream with firming and anti-wrinkle effects according to claim 3, characterized in that, The cream having firming and anti - wrinkle effects further contains at least one of composite polypeptide, recombinant collagen, skin conditioner, thickener, emulsifier, emollient, preservative, chelating agent, humectant, solvent.

5. The cream with firming and anti-wrinkle effects according to claim 4, characterized in that, The composite polypeptide is at least two of palmitoyl tripeptide - 1, palmitoyl tetrapeptide - 7, acetyl hexapeptide - 8, dipeptide diamino butyryl benzyl amide diacetate, carnosine, nonapeptide - 1; The recombinant collagen is at least one of recombinant type XVII collagen, recombinant type I collagen, recombinant type IV collagen, recombinant type V collagen, recombinant type VII collagen; The skin conditioner is at least one of polydeoxyribonucleotide, niacinamide, octanoyl hydroxamic acid, 3 - O - ethyl ascorbic acid; The thickener is at least one of sodium polyacrylate, acrylate / C10 - 30 alkanol acrylate cross - linked polymer, xanthan gum, carbomer, polyacrylamide; The emulsifier is at least one of PEG - 20 methyl glucoside sesquistearate, glyceryl stearate, sorbitan stearate, PEG - 100 stearate, cetearyl glucoside; The emollient is at least one of dicaprylyl carbonate, cetearyl alcohol, hydrogenated polyisobutene, phenyl trimethicone, tocopheryl acetate, C13 - 14 isoparaffin, jojoba seed oil, squalane, caprylic / capric triglyceride, ethylhexyl palmitate, shea butter, polydimethylsiloxane, lavender oil, grapefruit peel oil, ylang - ylang oil, lemon peel oil, rosa damascena flower oil; The preservative is at least one of p-hydroxyacetophenone, methylparaben, octanoyl hydroxamic acid, glyceryl caprylate, 1,2-pentanediol, phenoxyethanol, ethylhexylglycerin, and octyldodecanol; The chelating agent is at least one of disodium EDTA and tetrahydroxypropyl ethylenediamine; The humectant is at least one of β-glucan, glycerin, sodium polyglutamate, Tremella fuciformis Berk. fruit body extract, sodium hyaluronate, allantoin, butanediol, propylene glycol, and 1,2-hexanediol; The solvent is deionized water.

6. The preparation method of the cream with firming and anti-wrinkle effects according to claim 5, characterized in that The preparation method includes the following steps: Mix the firming and anti-wrinkle composition, composite polypeptide, recombinant collagen, skin conditioner, thickener, emulsifier, emollient, preservative, chelating agent, humectant, and solvent evenly to obtain the firming and anti-wrinkle cream.

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