An anti-wrinkle composition containing extract of *Vigna pubescens* flower and its preparation method thereof.

Abstract

This invention provides an anti-wrinkle composition containing extracts of *Velvetia scabra* flowers and its preparation method. The anti-wrinkle composition includes extracts of black willow bark, *Velvetia scabra* flowers, *Inula japonica* flowers, *Edelweiss* extract, and adenosine. When the above raw materials are combined in the composition, they can synergistically promote the secretion of type I collagen, and have significant effects in improving skin elasticity and reducing the formation of wrinkles.

Description

Technical Field

[0001] This invention relates to the field of cosmetics, specifically to an anti-wrinkle composition containing extracts of *Vigna pubescens* flowers and its preparation method. Background Technology

[0002] With the increasing demand for anti-aging skincare, the research and development of anti-wrinkle cosmetics has become an important direction in the cosmetics industry. The formation of wrinkles is closely related to the loss and degradation of collagen in the dermis, especially the reduction of type I collagen, which directly leads to decreased skin elasticity and wrinkle formation. Currently, anti-wrinkle cosmetics on the market mainly achieve antioxidant effects or mildly stimulate collagen synthesis through single ingredients (such as vitamin C, retinol, or plant extracts), but the following technical bottlenecks exist:

[0003] 1. Existing single active ingredients have limited anti-wrinkle effects and are difficult to achieve significant collagen increase. For example, although adenosine can promote fibroblast proliferation, its effect on increasing type I collagen secretion is insufficient when used alone; while plant extracts often fail to fully exert the synergistic effect between active substances due to their complex composition or unreasonable ratios.

[0004] 2. Traditional anti-wrinkle products suffer from significant safety and stability issues. Some chemically synthesized ingredients (such as retinoic acid derivatives) are prone to causing skin irritation or photosensitivity reactions, limiting the actual effectiveness of these products.

[0005] To address the aforementioned issues, there is an urgent need to provide an anti-aging composition that is highly safe and can significantly stimulate collagen secretion for consumers to choose from. Summary of the Invention

[0006] The purpose of this invention is to overcome the shortcomings of the existing technology and provide an anti-wrinkle composition containing extracts of *Vigna pubescens* flowers and its preparation method.

[0007] To achieve the above objectives, the technical solution adopted by the present invention is as follows: an anti-wrinkle composition containing extract of *Vallisneria natans*, the composition comprising extracts of *Salix matsudana* bark, *Vallisneria natans*, *Inula japonica*, *Edelweiss*, and adenosine, wherein the mass ratio of the extracts of *Salix matsudana* bark, *Vallisneria natans*, *Inula japonica*, *Edelweiss*, and adenosine is 1:(0.3-0.5):(0.7-0.9):(0.6-0.8):(0.1-0.3).

[0008] Preferably, the composition comprises black willow bark extract, wound-healing velvet flower extract, inula flower extract, alpine edelweiss extract, and adenosine, wherein the mass ratio of the black willow bark extract, wound-healing velvet flower extract, inula flower extract, alpine edelweiss extract, and adenosine is 1:0.4:0.8:0.7:0.2.

[0009] Research has shown that when the mass ratio of black willow bark extract, wound-healing velvet flower extract, inula flower extract, alpine edelweiss extract, and adenosine is 1:0.4:0.8:0.7:0.2, the synergistic effect among these extracts is better, resulting in higher secretion of type I collagen.

[0010] The present invention also provides the use of the anti-wrinkle composition containing the extract of *Vigna pubescens* in the preparation of cosmetics with anti-wrinkle effects.

[0011] Preferably, the cosmetic dosage form with anti-wrinkle effect is any one of ointment, cream, emulsion, water, oil, gel, powder, tablet, mud, patch, film, aerosol, spray, freeze-dried preparation or nano-preparation.

[0012] The present invention also provides an anti-wrinkle serum, the serum comprising the above-mentioned anti-wrinkle composition.

[0013] Preferably, the essence further includes moisturizers, emulsifiers, thickeners, preservatives, and solvents.

[0014] Preferably, the humectant includes at least one of glycerin, propylene glycol, butylene glycol, and sodium mannophosphate.

[0015] Preferably, the emulsifier includes at least one of glyceryl stearate and cetearyl alcohol.

[0016] Preferably, the thickener includes at least one of hydroxyethyl cellulose, xanthan gum, and carrageenan.

[0017] Preferably, the preservative includes at least one of methylparaben, p-hydroxyacetophenone, and sorbic acid.

[0018] Preferably, the solvent is deionized water.

[0019] The beneficial effects of this invention are as follows: The anti-wrinkle composition containing *Vigna pubescens* flower extract provided by this invention includes *Salix matsudana* bark extract, *Vigna pubescens* flower extract, *Inula japonica* flower extract, *Edelweiss* extract, and adenosine. Adenosine can bind to receptors on the surface of fibroblasts, promoting fibroblast proliferation and upregulating type I collagen gene transcription. Chlorogenic acid and caffeic acid derivatives in *Edelweiss* extract can enhance epidermal growth factor signaling, synergistically promoting fibroblast proliferation and increasing type I collagen secretion in conjunction with adenosine. Sesquiterpene lactones contained in *Inula japonica* flower extract can indirectly promote fibroblast metabolic activity, forming a "proliferation-metabolism" synergy with adenosine and *Edelweiss* extract. Flavonoids and polyphenols contained in *Salix matsudana* bark extract and *Vigna pubescens* flower extract can inhibit the release of inflammatory factors, indirectly inhibiting collagen degradation. The above five raw materials work synergistically to promote fibroblast secretion of type I collagen and reduce skin wrinkles. Detailed Implementation

[0020] To better illustrate the purpose, technical solution, and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0021] The raw materials used in this invention and their sources are as follows:

[0022] Black willow bark extract: purchased from Xi'an Lishi Biotechnology Co., Ltd., product number: LS-PG;

[0023] Healing hairy flower extract: purchased from Nanjing Institute of Wild Plants, product number: Healing hairy flower extract;

[0024] Inula japonica flower extract: purchased from Beijing Miaosen Biotechnology Co., Ltd., product number: Miaofujin;

[0025] Edelweiss extract: purchased from Weiming Shiguang Biotechnology Co., Ltd., product number: WaterReplent Edelweiss filtrate;

[0026] Adenosine: Purchased from Guangzhou Aoli Biotechnology Co., Ltd., product number: Adenosine AOS.

[0027] An anti-wrinkle composition containing extracts of *Vigna pubescens* flowers, the raw materials and their mass ratios, and the preparation method are as follows:

[0028] Composition 1: Composed of black willow bark extract, wound-healing velvet flower extract, inula flower extract, alpine edelweiss extract, and adenosine in a mass ratio of 1:0.4:0.8:0.7:0.2; Preparation method: Mix all raw materials in the composition evenly, seal and store in the dark.

[0029] Composition 2: Composed of black willow bark extract, wound-healing velvet flower extract, inula flower extract, alpine edelweiss extract, and adenosine in a mass ratio of 1:0.3:0.7:0.6:0.1; the preparation method is the same as that of composition 1.

[0030] Composition 3: Composed of black willow bark extract, wound-healing velvet flower extract, inula flower extract, alpine edelweiss extract, and adenosine in a mass ratio of 1:0.5:0.9:0.8:0.3; the preparation method is the same as that of composition 1.

[0031] Composition ①: Composed of extracts of *Velvetifolia scabra*, *Inula japonica*, *Edelweiss*, and adenosine in a mass ratio of 0.4:0.8:0.7:0.2; Preparation method: Mix all raw materials in the composition evenly, seal and store in the dark.

[0032] Composition ②: Composed of black willow bark extract, inula flower extract, edelweiss extract and adenosine in a mass ratio of 1:0.8:0.7:0.2; Preparation method: Mix all raw materials in the composition evenly, seal and store in the dark.

[0033] Composition ③: Composed of black willow bark extract, wound-healing velvet flower extract, alpine edelweiss extract, and adenosine in a mass ratio of 1:0.4:0.7:0.2; Preparation method: Mix all raw materials in the composition evenly, seal and store in the dark.

[0034] Composition ④: Composed of black willow bark extract, wound-healing velvet flower extract, inula flower extract, and adenosine in a mass ratio of 1:0.4:0.8:0.2; Preparation method: Mix all raw materials in the composition evenly, seal and store in the dark.

[0035] Composition ⑤: Composed of black willow bark extract, wound-healing velvet flower extract, inula flower extract, and alpine edelweiss extract in a mass ratio of 1:0.4:0.8:0.7; Preparation method: Mix all raw materials in the composition evenly, seal and store in the dark.

[0036] Composition ⑥: Composed of black willow bark extract, wound-healing velvet flower extract, inula flower extract, alpine edelweiss extract, and adenosine in a mass ratio of 1:0.8:0.4:0.2:0.7; Preparation method: Mix all raw materials in the composition evenly, seal and store in the dark.

[0037] The components, their mass percentages, and preparation method of the anti-wrinkle serum are as follows:

[0038] Serum 1:

[0039] Composition 1:5wt%

[0040] Sodium mannose phosphate: 2wt%

[0041] Glycerin: 6wt%

[0042] Cetearyl alcohol: 2wt%

[0043] Hydroxyethyl cellulose: 1 wt%

[0044] p-Hydroxyacetophenone: 0.2 wt%;

[0045] Add deionized water to bring the total to 100 wt%.

[0046] Preparation method: Sodium mannose phosphate, glycerin, cetearyl alcohol, p-hydroxyacetophenone and 1 / 2 deionized water are mixed evenly to obtain mixture 1; the composition is mixed evenly with 1 / 4 deionized water to obtain mixture 2; mixture 1, mixture 2, hydroxyethyl cellulose and the remaining deionized water are mixed evenly to obtain essence.

[0047] Serum 2:

[0048] Composition 2:5wt%

[0049] Sodium mannose phosphate: 2wt%

[0050] Glycerin: 6wt%

[0051] Cetearyl alcohol: 2wt%

[0052] Hydroxyethyl cellulose: 1 wt%

[0053] p-Hydroxyacetophenone: 0.2 wt%;

[0054] Add deionized water to bring the total to 100 wt%.

[0055] Preparation method: Sodium mannose phosphate, glycerin, cetearyl alcohol, p-hydroxyacetophenone and 1 / 2 deionized water are mixed evenly to obtain mixture 1; the composition is mixed evenly with 1 / 4 deionized water to obtain mixture 2; mixture 1, mixture 2, hydroxyethyl cellulose and the remaining deionized water are mixed evenly to obtain essence.

[0056] Serum 3:

[0057] Composition 3:5wt%

[0058] Sodium mannose phosphate: 2wt%

[0059] Glycerin: 6wt%

[0060] Cetearyl alcohol: 2wt%

[0061] Hydroxyethyl cellulose: 1 wt%

[0062] p-Hydroxyacetophenone: 0.2 wt%;

[0063] Add deionized water to bring the total to 100 wt%.

[0064] Preparation method: Sodium mannose phosphate, glycerin, cetearyl alcohol, p-hydroxyacetophenone and 1 / 2 deionized water are mixed evenly to obtain mixture 1; the composition is mixed evenly with 1 / 4 deionized water to obtain mixture 2; mixture 1, mixture 2, hydroxyethyl cellulose and the remaining deionized water are mixed evenly to obtain essence.

[0065] Serum ①:

[0066] Composition ①: 5wt%

[0067] Sodium mannose phosphate: 2wt%

[0068] Glycerin: 6wt%

[0069] Cetearyl alcohol: 2wt%

[0070] Hydroxyethyl cellulose: 1 wt%

[0071] p-Hydroxyacetophenone: 0.2 wt%;

[0072] Add deionized water to bring the total to 100 wt%.

[0073] Preparation method: Sodium mannose phosphate, glycerin, cetearyl alcohol, p-hydroxyacetophenone and 1 / 2 deionized water are mixed evenly to obtain mixture 1; the composition is mixed evenly with 1 / 4 deionized water to obtain mixture 2; mixture 1, mixture 2, hydroxyethyl cellulose and the remaining deionized water are mixed evenly to obtain essence.

[0074] Serum ②:

[0075] Composition ②: 5wt%

[0076] Sodium mannose phosphate: 2wt%

[0077] Glycerin: 6wt%

[0078] Cetearyl alcohol: 2wt%

[0079] Hydroxyethyl cellulose: 1 wt%

[0080] p-Hydroxyacetophenone: 0.2 wt%;

[0081] Add deionized water to bring the total to 100 wt%.

[0082] Preparation method: Sodium mannose phosphate, glycerin, cetearyl alcohol, p-hydroxyacetophenone and 1 / 2 deionized water are mixed evenly to obtain mixture 1; the composition is mixed evenly with 1 / 4 deionized water to obtain mixture 2; mixture 1, mixture 2, hydroxyethyl cellulose and the remaining deionized water are mixed evenly to obtain essence.

[0083] Serum ③:

[0084] Composition ③: 5wt%

[0085] Sodium mannose phosphate: 2wt%

[0086] Glycerin: 6wt%

[0087] Cetearyl alcohol: 2wt%

[0088] Hydroxyethyl cellulose: 1 wt%

[0089] p-Hydroxyacetophenone: 0.2 wt%;

[0090] Add deionized water to bring the total to 100 wt%.

[0091] Preparation method: Sodium mannose phosphate, glycerin, cetearyl alcohol, p-hydroxyacetophenone and 1 / 2 deionized water are mixed evenly to obtain mixture 1; the composition is mixed evenly with 1 / 4 deionized water to obtain mixture 2; mixture 1, mixture 2, hydroxyethyl cellulose and the remaining deionized water are mixed evenly to obtain essence.

[0092] Essence ④:

[0093] Composition ④: 5wt%

[0094] Sodium mannose phosphate: 2wt%

[0095] Glycerin: 6wt%

[0096] Cetearyl alcohol: 2wt%

[0097] Hydroxyethyl cellulose: 1 wt%

[0098] p-Hydroxyacetophenone: 0.2 wt%;

[0099] Add deionized water to bring the total to 100 wt%.

[0100] Preparation method: Sodium mannose phosphate, glycerin, cetearyl alcohol, p-hydroxyacetophenone and 1 / 2 deionized water are mixed evenly to obtain mixture 1; the composition is mixed evenly with 1 / 4 deionized water to obtain mixture 2; mixture 1, mixture 2, hydroxyethyl cellulose and the remaining deionized water are mixed evenly to obtain essence.

[0101] Essence ⑤:

[0102] Composition ⑤: 5wt%

[0103] Sodium mannose phosphate: 2wt%

[0104] Glycerin: 6wt%

[0105] Cetearyl alcohol: 2wt%

[0106] Hydroxyethyl cellulose: 1 wt%

[0107] p-Hydroxyacetophenone: 0.2 wt%;

[0108] Add deionized water to bring the total to 100 wt%.

[0109] Preparation method: Sodium mannose phosphate, glycerin, cetearyl alcohol, p-hydroxyacetophenone and 1 / 2 deionized water are mixed evenly to obtain mixture 1; the composition is mixed evenly with 1 / 4 deionized water to obtain mixture 2; mixture 1, mixture 2, hydroxyethyl cellulose and the remaining deionized water are mixed evenly to obtain essence.

[0110] Essence ⑥:

[0111] Composition ⑥: 5wt%

[0112] Sodium mannose phosphate: 2wt%

[0113] Glycerin: 6wt%

[0114] Cetearyl alcohol: 2wt%

[0115] Hydroxyethyl cellulose: 1 wt%

[0116] p-Hydroxyacetophenone: 0.2 wt%;

[0117] Add deionized water to bring the total to 100 wt%.

[0118] Preparation method: Sodium mannose phosphate, glycerin, cetearyl alcohol, p-hydroxyacetophenone and 1 / 2 deionized water are mixed evenly to obtain mixture 1; the composition is mixed evenly with 1 / 4 deionized water to obtain mixture 2; mixture 1, mixture 2, hydroxyethyl cellulose and the remaining deionized water are mixed evenly to obtain essence.

[0119] Blank serum:

[0120] Sodium mannose phosphate: 2wt%

[0121] Glycerin: 6wt%

[0122] Cetearyl alcohol: 2wt%

[0123] Hydroxyethyl cellulose: 1 wt%

[0124] p-Hydroxyacetophenone: 0.2 wt%;

[0125] Add deionized water to bring the total to 100 wt%.

[0126] Preparation method: Sodium mannose phosphate, glycerin, cetearyl alcohol, p-hydroxyacetophenone and 1 / 2 deionized water are mixed evenly to obtain mixture 1; mixture 1, hydroxyethyl cellulose and the remaining deionized water are mixed evenly to obtain essence.

[0127] Experiment 1: Effect of the composition on collagen secretion by fibroblasts

[0128] This experiment investigated the effect of the serums obtained in the examples and comparative examples on promoting collagen secretion. Specifically, it referred to T / SHRH 031-2020 "Test of Firming and Anti-wrinkle Efficacy of Cosmetics - Determination of Type I Collagen Content in In Vitro Fibroblasts". The composition was tested at 5 wt%, the solvent was cell culture medium, and the positive control drug was 100 ng / mL LTGFβ1. The test results are shown in Table 1.

[0129] Table 1. Determination of the upregulation effect of the composition on type I collagen secretion by fibroblasts.

[0130] Group Type I collagen upregulation rate / % Composition 1 33.22 Composition 2 32.71 Composition 3 32.26 Composition ① 19.38 Composition ② 17.67 Composition ③ 21.23 Composition ④ 24.14 Composition ⑤ 18.49 Composition ⑥ 27.31 positive group 34.19

[0131] As shown in Table 1, comparing compositions 1-3 with compositions ①-⑥, the anti-wrinkle compositions provided by this invention can significantly promote the secretion of type I collagen by fibroblasts. Specifically, comparing compositions 1-3 with composition ⑥, it can be seen that when the amounts of each ingredient in the composition are within the mass ratio range specified in this invention, the composition can significantly increase the secretion of type I collagen by fibroblasts; comparing compositions 1 with compositions ①-⑤, it can be seen that there is a significant synergistic effect among the ingredients in the anti-wrinkle compositions of this invention, and their combined use can significantly promote the secretion of type I collagen by fibroblasts.

[0132] Experiment 2: Human Efficacy Testing

[0133] Fifty individuals with wrinkles and healthy, undamaged skin, aged 35-40, were selected and divided into 10 groups of 5 people each. Each group used serums 1-3, 1-6, and a blank serum, respectively. Irritation tests and wrinkle improvement tests were conducted.

[0134] Irritation test: Place 0.020-0.025g of the serum into a patch applicator. Cover the patch applicator with non-irritating adhesive tape and apply it to the back or flexor side of the forearm of the subject. Gently press with the palm to ensure even adhesion to the skin surface. Leave on for 24 hours. Remove the patch applicator and observe the skin reaction 30 minutes after the pressure mark disappears. If the result is negative, observe again at 24 and 48 hours after the patch test.

[0135] Evaluation criteria:

[0136] Grade 0: Negative reaction;

[0137] Grade 1: Suspicious reaction, with only slight erythema;

[0138] Grade 2: Weak positive reaction, erythema, infiltration, edema, and possible papules;

[0139] Grade 3: Strong positive reaction, erythema, infiltration, edema, papules may be present, and the reaction may extend beyond the test area;

[0140] Grade 4: Extremely positive reaction, with obvious erythema, severe infiltration, edema, confluent herpes, and reaction extending beyond the test area.

[0141] Test results: All subjects showed negative skin reactions.

[0142] Wrinkle Improvement Test: After washing their face with water every morning, users take 2 mL of the essence and apply it evenly to their face. They use it once a day for two months without using any other skincare products during the trial.

[0143] The Derma TOP rapid skin imaging analysis system employs stripe projection measurement technology. Wrinkle parameters are obtained through primary localization using head and two ear sensors, secondary facial laser localization, camera image capture, rapid 3D skin imaging, and computer image processing. Wrinkle parameters, including the number, length, width, and depth of wrinkles, are obtained within a 2cm x 2cm area at the outer canthus of each subject's eyes. The wrinkle volume within this 2cm x 2cm area is calculated using an integral method. In the formula, n represents the number of wrinkles, and a, b, and c represent the length, width, and depth of the wrinkles, respectively. The sum of the values ​​for both eyes is taken. The volume of the wrinkles before the trial is V0, and the volume after two months of trial is recorded as V2.

[0144] Wrinkle improvement rate after two months of trial use = (V0-V2) / V0×100%.

[0145] The results are shown in Table 2.

[0146] Table 2 Results of Human Function Measurement

[0147] Group Wrinkle improvement rate / % Serum 1 16.26 Serum 2 15.57 Serum 3 15.39 Serum ① 8.65 Serum ② 7.87 Serum ③ 10.22 Essence ④ 11.34 Essence ⑤ 8.09 Essence ⑥ 12.42 Blank Essence 2.71

[0148] As shown in Table 2, the serum provided by this invention has a significant effect on improving wrinkles and is gentle and non-irritating. A comparison of serums 1-3 and the blank serum shows that the anti-wrinkle composition in this invention is the main anti-wrinkle active ingredient in the serum. A comparison of serums 1-3 and serum ⑥ shows that when the amounts of each ingredient in the anti-wrinkle composition of the serum are within the mass ratio range specified in this invention, its anti-wrinkle effect is better. A comparison of serum 1 and serums ①-⑤ shows that there is a significant synergistic effect among the ingredients in the anti-wrinkle composition of the serum, and their combined use can significantly improve the wrinkle improvement rate of the serum.

[0149] Finally, it should be noted that the above compositions and test examples are only used to illustrate the technical solutions of the present invention and not to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. An anti-wrinkle composition comprising an extract of Gypsophila oldhamiana Maxim. characterized in that, The composition is composed of black willow bark extract, Yishangmu flower extract, Xuanfuhua flower extract, Gaoshanhuangcao extract and adenosine, and the mass ratio of the black willow bark extract, Yishangmu flower extract, Xuanfuhua flower extract, Gaoshanhuangcao extract and adenosine is 1:(0.3-0.5):(0.7-0.9):(0.6-0.8):(0.1-0.3).

2. The composition of claim 1, wherein, The mass ratio of the black willow bark extract, Yishangmu flower extract, Xuanfuhua flower extract, Gaoshanhuangcao extract and adenosine is 1:0.4:0.8:0.7:0.

2.

3. Use of the anti-wrinkle composition containing Yishangmu flower extract according to any one of claims 1-2 in the preparation of a cosmetic product having anti-wrinkle efficacy.

4. Use according to claim 3, characterized in that, The cosmetic product having anti-wrinkle efficacy is any one of a cream, a cream, an emulsion, an aqueous agent, an oil agent, a gel, a powder, a tablet, a mud agent, a patch, a film agent, an aerosol agent, a spray agent, a freeze-dried preparation or a nano-preparation.

5. An essence having an anti-wrinkle effect, characterized by, The essence includes the anti-wrinkle composition containing Yishangmu flower extract according to any one of claims 1-2.

6. The serum of claim 5, wherein the serum is characterized by, The essence further includes a humectant, an emulsifier, a thickening agent, a preservative and a solvent.

7. The serum of claim 6, wherein the serum is characterized by, The humectant includes at least one of glycerol, propylene glycol, butylene glycol and sodium mannose phosphate.

8. The serum of claim 6, wherein the serum is characterized by, The emulsifier includes at least one of glycerol stearate and cetyl stearyl alcohol.

9. The serum of claim 6, wherein, The thickening agent includes at least one of hydroxyethyl cellulose, xanthan gum and carrageenan; the preservative includes at least one of hydroxybenzoic acid ester, p-hydroxyacetophenone and sorbic acid; and the solvent is deionized water.