Skin-whitening tea light-resisting composition specially researched for photosensitive skin and application of skin-whitening tea light-resisting composition

Abstract

The invention relates to the technical field of cosmetics, in particular to a skin-whitening tea light-resisting composition specially researched for photosensitizing skin and application of the skin-whitening tea light-resisting composition. The tea yuguang composition disclosed by the invention is prepared from the following components in parts by weight: 0.5 to 1 part of theanine and 1 to 13 parts of snow field extract, the snow field extract comprises a leontopodium alpinum flower / leaf extract, a snow lotus herb extract and a centella asiatica extract. The theanine, the leontopodium alpinum flower / leaf extract, the saussurea involucrata extract and the centella asiatica extract are combined to accurately strike different stages of the light injury, and full-path coverage of the light injury from early signal-middle inflammation-later pigmentation and structural injury is realized. The leontopodium leontopodum, theanine and centella jointly construct a low-oxidation and low-inflammation stable environment, the whitening efficiency of the snow lotus herb is greatly improved, multi-target and staged synergistic effect is truly achieved, and 1 + 1 + 1 + gt is achieved; gt; and 4, the composition has a composite repairing effect and is especially suitable for light-sensitive people.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, and in particular to a whitening tea composition specifically developed for photosensitive skin and its application. Background Technology

[0002] Photodamage is a series of pathological changes in the skin triggered by ultraviolet (UV) radiation. It encompasses immediate inflammatory responses (such as redness and stinging), medium-term oxidative damage (such as the accumulation of reactive oxygen species), and long-term pigmentation and barrier function decline, with a particularly significant impact on photosensitivity individuals. Photosensitivity is defined as skin that exhibits obvious redness, peeling, itching, and pain after only 15-30 minutes of exposure to moderate-intensity UV radiation (such as UV index 7-9).

[0003] Current technologies for protecting and repairing skin from photodamage mostly rely on single active ingredients or simple combinations, making it difficult to comprehensively combat photodamage. Moreover, existing technologies are mostly designed to address the photodamage needs of the general population, and their effectiveness is very poor for photosensitive individuals, whose skin has a significantly lower tolerance to ultraviolet radiation than the average person. The skin of photosensitive individuals is like a highly sensitive "alarm system," where even a negligible amount of ultraviolet radiation for the average person is enough to trigger severe inflammatory reactions and damage on their skin.

[0004] Therefore, current technologies for protecting and repairing skin photodamage are limited by the single or combined nature of their active ingredients, making it impossible to effectively intervene in the entire chain of photodamage development. In particular, they are unable to meet the needs of photosensitive individuals for efficient and comprehensive photoprotection and repair, and there is an urgent need to develop a composition suitable for photosensitive individuals. Summary of the Invention

[0005] The purpose of this invention is to overcome the shortcomings of the existing technology and provide a whitening tea composition for photosensitive skin and its application.

[0006] To achieve the above objectives, the technical solution adopted by the present invention is as follows: In a first aspect, the present invention provides a tea-based composition comprising the following components in parts by weight: 0.5-1 parts of theanine and 1.5-13 parts of snow-covered plateau extract; wherein the snow-covered plateau extract comprises Edelweiss flower / leaf extract, snow lotus extract, and Centella asiatica extract.

[0007] This invention has developed a tea-based light-protecting composition specifically for people with photosensitivity. It combines four ingredients: theanine, edelweiss flower / leaf extract, snow lotus extract, and centella asiatica extract. This combination precisely targets different stages of light damage, achieving full-pathway coverage of light damage from "early signaling to mid-stage inflammation to late-stage pigmentation and structural damage".

[0008] Theanine is a natural amino acid known for its powerful soothing and relaxing properties. It directly combats stress responses in nerve endings and skin cells, quickly reducing burning, stinging, and redness caused by external stimuli (such as UV rays and pollution), providing dual relief in both sensation and appearance.

[0009] Edelweiss flower / leaf extract, also known as snow edelweiss, grows in harsh environments with high altitudes and strong ultraviolet radiation. It has evolved an extremely powerful antioxidant defense system and is rich in polyphenols and chlorogenic acid. The abundant polyphenolic compounds (such as chlorogenic acid) can effectively neutralize excess free radicals generated after ultraviolet radiation, block the chain reaction of oxidative stress, and prevent the degradation of collagen and elastin, thereby preventing photoaging.

[0010] Snow lotus extract grows in the extremely cold and oxygen-deficient environment of snow-capped mountains. Its extract is rich in unique flavonoids, alkaloids, and other active ingredients. It effectively inhibits tyrosinase activity and kills rice particles, directly targeting the core pathway of melanin production, reducing melanin synthesis at its source. This makes it a key active ingredient for brightening skin tone and fading dark spots.

[0011] Centella asiatica extract is renowned for its powerful repairing and soothing properties, promoting fibroblast proliferation and collagen synthesis, and accelerating barrier repair and wound healing. It also alleviates skin inflammation, improves redness and sensitivity, and strengthens skin tolerance, playing a crucial role, especially in the repair phase after photodamage.

[0012] Antioxidant extracts of Edelweiss flower / leaf alone cannot completely soothe stinging and redness; theanine, with its simple anti-inflammatory properties, is ineffective against existing melanin; and snow lotus or centella asiatica, which focus solely on whitening, lack control over the oxidative and inflammatory environment, resulting in limited long-term effects. While combining two ingredients can compensate for some shortcomings—for example, antioxidants and anti-inflammatories can alleviate initial damage—they cannot block melanin production or promote deep repair, often leaving behind dullness and fragility. This invention, through extensive research, has discovered that only by combining all four can the oxidation chain be simultaneously blocked (Edelweiss flower / leaf extract), providing immediate soothing (theanine), deep repair (centella asiatica extract), and melanin inhibition (snow lotus extract). This achieves a complete reversal of the pathway from the signaling origin to the damage's end, truly achieving highly efficient and long-lasting photoprotection and repair.

[0013] This invention achieves multi-stage precise intervention against photodamage through a four-pronged mechanism: theanine (rapidly soothing and anti-inflammatory), edelweiss flower / leaf extract (highly effective antioxidant), snow lotus extract (source-level melanin inhibition and brightening), and centella asiatica extract (barrier repair and anti-inflammatory). Theanine rapidly relieves burning and stinging pain, edelweiss scavenge free radicals and block oxidative stress, centella asiatica strengthens and repairs the skin barrier and reduces inflammation, and snow lotus effectively inhibits melanin production in low-oxygen and low-inflammatory environments. The four ingredients work together to cover the entire pathway from stress signals, inflammatory responses, barrier damage to pigmentation. No single ingredient or combination of any two cannot achieve such comprehensive photoprotection and repair effects, truly achieving a synergistic effect of 1+1+1+1>>4.

[0014] Preferably, the tea-based composition comprises the following components in parts by weight: 0.7 parts theanine and 1.5-13 parts snow extract.

[0015] Preferably, in the snow-covered region extract, the mass ratio of Edelweiss flower / leaf extract, snow lotus extract, and centella asiatica extract is Edelweiss flower / leaf extract: snow lotus extract: centella asiatica extract = (0.5-5): (0.5-5): (0.5-3).

[0016] More preferably, in the snow-covered plateau extract, the mass ratio of Edelweiss flower / leaf extract, snow lotus extract, and centella asiatica extract is Edelweiss flower / leaf extract: snow lotus extract: centella asiatica extract = (1.5-5): (1.5-5): (1-3).

[0017] More preferably, in the snow-covered region extract, the mass ratio of Edelweiss flower / leaf extract, snow lotus extract, and centella asiatica extract is Edelweiss flower / leaf extract: snow lotus extract: centella asiatica extract = 3:3:2.

[0018] Secondly, the present invention provides the application of the above-mentioned tea-based light-protecting composition in the preparation of cosmetics.

[0019] Preferably, the cosmetic product has the effects of whitening the skin, protecting against photodamage, and soothing the skin.

[0020] Thirdly, the present invention provides a cosmetic comprising the above-mentioned tea-based light-repelling composition and excipients; the excipients comprising at least one of glycerin, butylene glycol, xanthan gum, panthenol, phenoxyethanol, triethanolamine, and water.

[0021] Preferably, the excipients comprise the following components in parts by weight: 4-6 parts glycerin, 3-5 parts butylene glycol, 0.1-0.2 parts xanthan gum, 0.5-2 parts panthenol, 0.5-1 part phenoxyethanol, 0.05-0.15 parts triethanolamine, and water to make up to 100 parts by weight of the cosmetic.

[0022] Preferably, the excipients comprise the following components in parts by weight: 5 parts glycerin, 4 parts butylene glycol, 0.15 parts xanthan gum, 1 part panthenol, 0.8 parts phenoxyethanol, 0.1 parts triethanolamine, and water to make up to 100 parts by weight of the cosmetic.

[0023] Preferably, the cosmetic is a serum.

[0024] Fourthly, the present invention provides a method for preparing the above-mentioned cosmetic, comprising the following steps: S1: Xanthan gum is premixed with a portion of glycerol to obtain a xanthan gum predispersed slurry; S2: Mix water, the remaining glycerol and butylene glycol, and stir until the mixture is homogeneous; add the xanthan gum predispersed slurry prepared in step S1, heat and stir until the mixture is homogeneous to obtain mixture A; S3: Cool down, stir and add triethanolamine to mixture A, adjust the pH value to obtain mixture B; S4: Add theanine, edelweiss flower / leaf extract, snow lotus extract, centella asiatica extract and panthenol to mixture B in sequence, stir, then add phenoxyethanol, stir evenly and cool to room temperature to obtain the cosmetic product.

[0025] Preferably, in steps S1 to S4, the stirring speed is 400-600 rpm, more preferably 500 rpm.

[0026] Preferably, in step S3, the temperature is adjusted to 40°C.

[0027] Preferably, in step S4, the process temperature is adjusted to 40°C.

[0028] Preferably, the preparation method specifically includes the following steps: (1) Mix xanthan gum with 1 part glycerol in a small beaker and stir with a glass rod to form a uniform slurry without any dry powder particles to obtain xanthan gum predispersed slurry for later use; (2) Mix water, the remaining glycerol and butanediol, set the speed to 300-400 rpm, and stir for 5 minutes until the mixture is uniform; add the xanthan gum predispersed slurry prepared in step S1, heat it appropriately to 40-45°C, increase the speed to 400-600 rpm, and continue stirring for about 3-5 minutes until the system is completely clear and transparent without any colloidal particles. You can appropriately heat it to 40-45°C to accelerate hydration and obtain mixture A; (3) Cool the mixture A to below 40°C, and slowly add triethanolamine while stirring at 400-600 rpm. Observe the viscosity change at any time until the system becomes a clear and transparent gel and the pH value is stable between 5.5 and 6.0. Stop adding triethanolamine to obtain mixture B. (4) Keep the temperature of mixture B <40°C, stir the theanine, edelweiss flower / leaf extract, snow lotus extract, centella asiatica extract and panthenol in the tea light composition at 400-600 rpm, then add phenoxyethanol, stir evenly and cool to room temperature to obtain the cosmetic.

[0029] The beneficial effects of this invention are as follows: This invention develops a tea-based light-protecting composition for people with photosensitivity. It comprehensively utilizes theanine, edelweiss flower / leaf extract, snow lotus extract, and centella asiatica extract, combining these four ingredients to achieve the following breakthroughs in efficacy: 1. Precisely targets different stages of photodamage, covering multiple aspects such as oxidative stress, inflammatory response, DNA damage, and barrier impairment. The formulation of this invention achieves full-pathway coverage of photodamage from "early signaling—mid-stage inflammation—late-stage pigmentation and structural damage" through the synergistic effect of four components.

[0030] 2. Edelweiss, together with theanine and centella asiatica, creates a stable environment with low oxidation and low inflammation, which greatly enhances the whitening efficacy of snow lotus, truly achieving multi-target and phased synergistic effects, and realizing a compound repair effect of 1+1+1+1>>4. Attached Figure Description

[0031] Figure 1 The essence prepared in Application Example 5 of this invention.

[0032] Figure 2 Images of the compositions prepared in Examples 4 and 5.

[0033] Figure 3 The image shows the results of the DNA damage and repair test (circled in red; the wells contain cells, test samples, and test reagents).

[0034] Figure 4 This is a graph showing the results of a fluorescence experiment on DNA damage and repair. Detailed Implementation

[0035] To better illustrate the purpose, technical solution, and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0036] Unless otherwise specified, the experimental methods used in this invention are conventional methods, and the materials and reagents used are commercially available products that can be obtained through commercial channels.

[0037] The raw materials used in this invention are sourced from: Theanine was purchased from Guangzhou Yachun Cosmetics Manufacturing Co., Ltd.

[0038] Edelweiss flower / leaf extract, purchased from Haoyu (Guangzhou) Cosmetics Manufacturing Co., Ltd.

[0039] Snow lotus extract, purchased from Haoyu (Guangzhou) Cosmetics Manufacturing Co., Ltd.

[0040] Centella asiatica extract, purchased from Haoyu (Guangzhou) Cosmetics Manufacturing Co., Ltd.

[0041] Examples 1-7 Examples 1-7 each provide a tea-based light-protecting composition ( Figure 2 Its component composition (parts by weight) is shown in Table 1.

[0042] Table 1 Comparative Examples 1-8 Comparative Examples 1-8 each provide a tea-light composition, the composition of which (parts by weight) is shown in Table 2.

[0043] Table 2 Comparative Example 9 Comparative Example 9 provides a tea-based light-protecting composition. The only difference between the tea-based light-protecting composition of this comparative example and Example 5 is that an equal amount of tea polyphenols are used to replace the theanine in Example 5, and the rest is the same as Example 5.

[0044] Comparative Example 10 Comparative Example 10 provides a tea-based composition for purifying light. The only difference between the tea-based composition for purifying light in this comparative example and Example 5 is that an equal part by weight of Rhodiola rosea extract is used instead of the Edelweiss flower / leaf extract in Example 5. The rest is the same as in Example 5.

[0045] Comparative Example 11 Comparative Example 11 provides a tea-based composition for purifying light. The only difference between this tea-based composition and Example 5 is that an equal amount of ellagic acid is used instead of the snow lotus extract in Example 5. The rest is the same as in Example 5.

[0046] Comparative Example 12 Comparative Example 12 provides a tea-based composition for purifying light. The only difference between this tea-based composition and Example 5 is that an equal amount of immortelle extract is used instead of the centella asiatica extract in Example 5. The rest is the same as in Example 5.

[0047] Application Examples 1-7 Application Examples 1-7 respectively provide an essence containing the tea-infused light-repelling composition described in Examples 1-7. Figure 1 The specific amounts of the tea-infused light-emitting composition included in each application example are shown in Table 3.

[0048] The serum also includes the following excipients: 5% glycerin, 4% butylene glycol, 0.15% xanthan gum, 1% panthenol, 0.8% phenoxyethanol, 0.1% triethanolamine, and water to make up to 100% of the total mass of the serum.

[0049] Table 3 The preparation method of the serum includes the following steps: (1) Mix xanthan gum with 1% glycerol in a small beaker and stir with a glass rod to form a uniform slurry without any dry powder particles to obtain xanthan gum predispersed slurry for later use; (2) Mix water, the remaining glycerol and butanediol, set the speed to 300-400 rpm, and stir for 5 minutes until the mixture is uniform; add the xanthan gum predispersed slurry prepared in step S1, heat it appropriately to 40-45°C, increase the speed to 400-600 rpm, and continue stirring for about 3-5 minutes until the system is completely clear and transparent without any colloidal particles. You can appropriately heat it to 40-45°C to accelerate hydration and obtain mixture A; (3) Cool the mixture A to below 40°C, and slowly add triethanolamine while stirring at 400-600 rpm. Observe the viscosity change at any time until the system becomes a clear and transparent gel and the pH value is stable between 5.5 and 6.0. Stop adding triethanolamine to obtain mixture B. (4) Keep the temperature of mixture B <40°C, stir the theanine, edelweiss flower / leaf extract, snow lotus extract, centella asiatica extract and panthenol in the tea light composition at 400-600 rpm, then add phenoxyethanol, stir evenly and cool to room temperature to obtain the essence.

[0050] Application of Comparative Examples 1-12 Comparative Examples 1-12 each provided an essence containing the Tea-Radiance Composition described in Comparative Examples 1-12. The specific amount of Tea-Radiance Composition added to each comparative example is shown in Table 4.

[0051] The serum also includes the following excipients: 5% glycerin, 4% butylene glycol, 0.15% xanthan gum, 1% panthenol, 0.8% phenoxyethanol, 0.1% triethanolamine, and water to make up to 100% of the total mass of the serum.

[0052] The preparation method of the serum is the same as in the application example.

[0053] Table 4 Test Example 1: DNA Damage Repair Efficacy Test 1. Test samples: test group (tea light composition of Examples 1-7 and Comparative Examples 1-12), positive control group and negative control group.

[0054] 2. Instruments: LAICA SP8 laser confocal microscope, Sartiorius BSA-224S electronic balance.

[0055] 3. Main reagents: DNA damage detection kit (γ-H2AX immunofluorescence assay, Shanghai Beyotime Biotechnology Co., Ltd., C2035S).

[0056] 4. Testing Principle: Reactive oxygen species and ultraviolet (UV) radiation can cause DNA double-strand breaks, considered the most severe forms of DNA damage. H2A histone family member X (H2AX) is a variant of chromosomal histone H2A. When a cell's DNA double helix breaks, H2AX undergoes rapid phosphorylation, forming phosphorylated H2AX (γ-H2AX). The formation of γ-H2AX is a rapid response to DNA damage; therefore, γ-H2AX is an important DNA damage marker widely used in DNA damage and apoptosis research. The level of γ-H2AX effectively reflects the extent of DNA damage; the stronger the fluorescence (green) intensity, the more severe the DNA damage. Ultraviolet (UV) radiation: Ultraviolet radiation is a common DNA damage inducing factor. Exposing cells to a certain dose of UV radiation simulates UV damage to cellular DNA. UV radiation can cause pyrimidine bases (such as thymine) in DNA molecules to form pyrimidine dimers, leading to distortion of the DNA double helix structure, which in turn triggers DNA double-strand breaks and other damage, resulting in elevated γ-H2AX levels.

[0057] 5. Testing Procedure: Exponentially growing human skin fibroblasts (HFB) cells were divided into groups of 5 × 10⁻⁶ cells. 4Cells were seeded in 10 mm laser confocal microscopy dishes and fixed for 24 h in a 5% CO2, 37°C incubator. The positive control and test groups were then irradiated with UV light for 10 min. The test groups were given 100 μg / mL of the test sample and incubated together for 24 h. The negative control group used normal cell culture reagents without any added factors that could cause DNA damage. The culture medium containing the test sample was removed, and the cells were washed twice with PBS buffer. 1 mL of immunostaining blocking solution was added. The immunostaining blocking solution was removed, and γ-H2AX rabbit monoclonal antibody was added. The cells were incubated at room temperature for 1 h, then the γ-H2AX rabbit monoclonal antibody was removed, and the cells were washed twice with PBS buffer. Anti-rabbit 488 was added, and the cells were incubated at room temperature for 1 h, then the anti-rabbit 488 was removed, and the cells were washed twice with PBS buffer. 100 μL of DAPI was added, and the cells were incubated for 30 min. The treated cells were observed under a laser confocal microscope with an excitation wavelength of 488 nm and an emission wavelength of 525 nm. The results of the DNA damage repair efficacy test are as follows: Figure 3-4 As shown in Table 5.

[0058] Table 5 Test Example 2: Test for Inhibition of Tyrosinase Activity 1. Test samples: The serums used in Examples 1-7 and Comparative Examples 1-12.

[0059] 2. Experimental Principle: Tyrosinase is the rate-limiting enzyme in the melanin synthesis pathway. It mainly affects melanin production by influencing the conversion of tyrosine to dopa and the oxidation of dopa to dopaquinone. The principle is that tyrosine or dopa is converted to dopaquinone under the action of tyrosinase. This reaction is a colorimetric reaction, and by measuring the change in absorbance before and after the reaction using a colorimetric method, the degree of inhibition of tyrosinase activity by different serums can be determined, thereby evaluating the whitening efficacy of different lotions.

[0060] 3. Reagents and materials: (1) Prepare 0.05 mol / L PBS buffer (phosphate buffer solution, pH=6.8): Solution A: Weigh 7.099 g of disodium hydrogen phosphate and add distilled water to 1000 mL to obtain a 0.05 mol / L Na2HPO4 solution; Solution B: Weigh 6.803 g of potassium dihydrogen phosphate and add distilled water to 1000 mL to obtain a 0.05 mol / L KH2PO4 solution; Mix 50 mL of solution A and 50 mL of solution B to obtain a 0.05 mol / L PBS buffer (pH=6.8).

[0061] (2) Preparation of tyrosinase solution: Prepare tyrosinase (25 kU) to 100 U / mL using the PBS buffer from step (1) and prepare immediately before use.

[0062] (3) Prepare dopa solution: Weigh 0.04 g of dopa and dissolve it in 40 mL of PBS buffer from step (1). Store in the dark.

[0063] (4) Preparation of α-arbutin: Dilute α-arbutin to 100 μM with the PBS buffer from step (1).

[0064] 4. Instruments and equipment: analytical balance (accurate to 0.0001 g), 1 L volumetric flask, 50 mL graduated cylinder, 1000 μL pipette, constant temperature water bath, UV-Vis spectrophotometer.

[0065] 5. Measurement procedure: Prepare the test solution according to Table 6, and add the test sample, PBS buffer and tyrosinase solution to the test tube in sequence.

[0066] Table 6 After preparing the test solution, incubate the test tube in a 37°C water bath for 10 min. Then, add 2 mL of dopa solution and react for 5 min. Measure the absorbance at 475 nm. The absolute difference between two independent measurements obtained under repeatability conditions should not exceed 10% of the arithmetic mean. Calculate the tyrosinase inhibition rate (%) according to formula (1): Tyrosinase inhibition rate (%) = [1 - (OD)] C -OD D ) / (OD A -OD B )]×100%——Formula (1) In the above formula, OD A The absorbance value of the enzyme solution control group is OD. B The absorbance value of the enzyme solution control blank group is OD. C The absorbance value of the sample group, OD D This is the absorbance value of the blank control in the sample group.

[0067] The results are shown in Table 7.

[0068] Table 7 The results showed that the essence containing the tea-based brightening composition prepared in the application examples of the present invention could significantly inhibit the activity of tyrosinase and had excellent brightening and whitening effects, with application example 5 showing the best results.

[0069] Test Example 3: Hyaluronidase Test 1. Test samples: The serums prepared using Examples 1-7 and Comparative Examples 1-12.

[0070] 2. Experimental Principle: Hyaluronidase is an endogenous aminohexosidase that causes the breakdown of macromolecular hyaluronic acid. Under the action of hyaluronidase, macromolecular hyaluronic acid is broken down to produce N-acetaminophen. Hyaluronidase can hydrolyze the 1,4-glycosidic bond between β-N-acetylglucosamine and D-glucuronic acid in hyaluronic acid to obtain β-N-acetylglucosamine. β-N-acetylglucosamine can condense with acetylacetone under alkaline conditions to form the chromogen 2-methyl-3-acetylpyrrole derivative. The chromogen reacts with dimethylaminobenzaldehyde in concentrated hydrochloric acid-ethanol to produce color. Therefore, measuring the N-acetaminophen content in the reaction system can indirectly reflect the activity of hyaluronidase. Hyaluronidase is strongly correlated with inflammation; therefore, inhibiting hyaluronidase activity is used as an indicator for studying anti-inflammatory effects.

[0071] 3. Solution preparation (1) Acetic acid-sodium acetate buffer (pH=5.6): Measure 1.155 mL of glacial acetic acid and dilute it to 100 mL. Mix well to obtain an acetic acid solution. Weigh 2.72 g of sodium acetate, dissolve it in water, and make up to 100 mL to obtain a sodium acetate solution. Add 4.8 mL of acetic acid solution to 45.2 mL of sodium acetate solution, mix well, and make up to 100 mL to obtain an acetate-sodium acetate buffer (pH=5.6).

[0072] (2) Sodium hyaluronate solution: Weigh 0.0050 g of sodium hyaluronate and dissolve it in 10 mL of acetate-sodium acetate buffer (pH=5.6) to prepare a sodium hyaluronate solution with a mass concentration of 0.5 mg / mL.

[0073] (3) Ehrlich reagent: Weigh 0.8 g of p-dimethylaminobenzaldehyde and dissolve it in 15 mL of concentrated hydrochloric acid and 15 mL of anhydrous ethanol. Store in the dark.

[0074] (4) CaCl2 solution: Weigh 2.8 g of anhydrous CaCl2 and dissolve it in 10 mL of deionized water, and sonicate until completely dissolved.

[0075] (5) NaOH solution: Weigh 0.16 g of NaOH and dissolve it in 10 mL of deionized water, and sonicate until completely dissolved.

[0076] (6) Hyaluronidase solution: Weigh 0.0100 g of hyaluronidase and dissolve it in 4 mL of acetate-sodium acetate buffer (pH=5.6) to prepare hyaluronidase solution. The working concentration is 1250 U / mL. Prepare before use.

[0077] (7) Acetylacetone solution: Dissolve 3.5 mL of acetylacetone in 50 mL of sodium carbonate solution (1.0 mol / L) to obtain an acetylacetone solution, which should be prepared before use.

[0078] 4. Adding samples Add samples in the order shown in Table 8 below.

[0079] Table 8 5. Data Analysis (1) The formula for calculating the inhibition rate is shown in equation (2): In the above formula: A: Absorbance value of control solution (acetic acid-sodium acetate buffer (pH=5.6) instead of essence); B: Absorbance value of blank control solution (acetic acid-sodium acetate buffer (pH=5.6) replaces sample solution and hyaluronidase solution); C: Absorbance value of the sample solution; D: Absorbance value of blank sample solution (acetic acid-sodium acetate buffer (pH=5.6) instead of hyaluronidase solution).

[0080] Table 9 The test results are shown in Table 9. Compared with the comparative examples, the essence containing the tea-based light-dispelling composition prepared in the application examples of this invention significantly inhibits hyaluronidase activity, thus exhibiting a good soothing effect. Among them, application example 5 showed the best effect, inhibiting hyaluronidase activity and achieving a soothing effect.

[0081] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A tea-based light-repelling composition, characterized in that, It includes the following components in parts by weight: 0.5-1 part theanine and 1.5-13 parts snow extract; the snow extract includes edelweiss flower / leaf extract, snow lotus extract and centella asiatica extract.

2. The tea-infused light-repelling composition as described in claim 1, characterized in that, It includes the following components in parts by weight: 0.7 parts theanine and 1.5-13 parts snow extract.

3. The tea-light-protecting composition as described in claim 1, characterized in that, In the snow-covered region extract, the mass ratio of Edelweiss flower / leaf extract, snow lotus extract, and centella asiatica extract is Edelweiss flower / leaf extract: snow lotus extract: centella asiatica extract = (0.5-5): (0.5-5): (0.5-3).

4. The tea-light-protecting composition as described in claim 3, characterized in that, In the snow-covered region extract, the mass ratio of Edelweiss flower / leaf extract, snow lotus extract, and centella asiatica extract is Edelweiss flower / leaf extract: snow lotus extract: centella asiatica extract = (1.5-5): (1.5-5): (1-3).

5. The tea-light-protecting composition as described in claim 4, characterized in that, In the snow-covered region extract, the mass ratio of Edelweiss flower / leaf extract, snow lotus extract, and centella asiatica extract is Edelweiss flower / leaf extract: snow lotus extract: centella asiatica extract = 3:3:

2.

6. The use of the tea-based cosmetic composition according to any one of claims 1-5 in the preparation of cosmetics.

7. A cosmetic product, characterized in that, The cosmetic comprises the tea-based composition and excipients as described in any one of claims 1-5; the excipients include at least one of glycerin, butylene glycol, xanthan gum, panthenol, phenoxyethanol, triethanolamine, and water.

8. The cosmetic product as described in claim 7, characterized in that, The excipients comprise the following components in parts by weight: 4-6 parts glycerin, 3-5 parts butylene glycol, 0.1-0.2 parts xanthan gum, 0.5-2 parts panthenol, 0.5-1 part phenoxyethanol, 0.05-0.15 parts triethanolamine, and water to make up to 100 parts by weight of the cosmetic.

9. The cosmetic product as described in claim 7, characterized in that, The cosmetic product in question is a serum.

10. A method for preparing a cosmetic product as described in any one of claims 7-9, characterized in that, Includes the following steps: S1: Xanthan gum is premixed with a portion of glycerol to obtain a xanthan gum predispersed slurry; S2: Mix water, the remaining glycerol and butylene glycol, and stir until the mixture is homogeneous; add the xanthan gum predispersed slurry prepared in step S1, heat and stir until the mixture is homogeneous to obtain mixture A; S3: Cool down, stir and add triethanolamine to mixture A, adjust the pH value to obtain mixture B; S4: Add theanine, edelweiss flower / leaf extract, snow lotus extract, centella asiatica extract and panthenol to mixture B in sequence, stir, then add phenoxyethanol, stir evenly and cool to room temperature to obtain the cosmetic product.

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