Microbial inhibition composition as well as preparation method and application thereof

Abstract

The invention relates to the technical field of microorganisms, and particularly discloses a preparation method of a microbial inhibition composition, the microbial inhibition composition comprises a microbial carrier, the microbial carrier is one of Escherichia coli and Staphylococcus aureus, and the volume ratio of the microbial carrier is 70-90%; the antibiotic drug is used for being loaded on the microbial carrier to play a role in inhibiting the microbial carrier; and a calcium-containing solution. According to the preparation method of the microbial inhibition composition provided by the invention, the inactivated bacteria are used as an antibiotic carrier, and the purpose of efficient and lasting antibacterial is achieved by utilizing the rapid and short-acting antibacterial effect of the antibiotics and the long-acting antibacterial effect of the inactivated microorganisms to stimulate immune reaction; by arranging a lifting part and an intermittent driving part, a traction part drives a sealing plate to rise to a set height every time a set time is reached, at the moment, a separation barrel is in an open state, a top-layer microbial carrier enters the separation barrel after being inactivated by an ultraviolet lamp, and the operation is repeated in this way; therefore, all the microbial carriers are uniformly radiated by the ultraviolet lamp.

Description

Technical Field

[0001] The present invention relates to the field of microbial technology, and in particular to a microbial inhibition composition and a preparation method and application thereof. Background Art

[0002] Microbial inhibitory compositions refer to a class of chemical or biological agents used to inhibit or kill microorganisms (such as bacteria, fungi and viruses). These compositions are usually composed of multiple ingredients, the purpose of which is to enhance the inhibitory effect or provide a broad spectrum of antimicrobial activity. Microbial inhibitory compositions are widely used in the fields of food preservation, agriculture, medicine, cosmetics and water treatment. The main ingredients may include antibiotics, antifungal agents, disinfectants, etc.

[0003] However, the biofilm in the infection model prevents many clinical therapeutic drugs, such as antibiotics, from fully exerting their efficacy. This is because the biofilm itself has a low response to antibiotics, and bacteria treated with antibiotics alone can often still exist or survive. In addition, existing bacterial inactivation technologies have defects. Taking ultraviolet light inactivation as an example, the irradiation depth of ultraviolet light is limited, and the microorganisms at the bottom are exposed to less ultraviolet light energy, making it impossible to ensure that the microorganisms in this area are completely inactivated. Summary of the invention

[0004] The purpose of the present invention is to provide a method for preparing a microorganism inhibitory composition to solve the above-mentioned deficiencies in the prior art.

[0005] In order to achieve the above object, the present invention provides the following technical solutions: A microbial inhibition composition comprises a microbial carrier, wherein the microbial carrier is one of Escherichia coli and Staphylococcus aureus, accounting for 70-90% by volume; an antibiotic drug, wherein the antibiotic drug is used to be loaded on the microbial carrier to inhibit the microbial carrier; and a calcium-containing solution.

[0006] A method for preparing a microorganism inhibitory composition, Raw material preparation; The microbial carrier suspension and the calcium-containing solution are mixed evenly by a mixing instrument to obtain a microbial carrier calcium overload mixed solution; The microbial carrier calcium overload mixed solution is co-incubated in an ice bath for 25-35 minutes to obtain a calcium overloaded microbial carrier; Adding an inhibitory drug to the calcium overloaded microbial carrier to obtain a drug-loaded microbial carrier; The drug-loaded microbial carrier is placed in an ultraviolet irradiation box and irradiated with ultraviolet light for inactivation; A separation cylinder is fixedly connected to the inner bottom of the ultraviolet irradiation box, and an opening and closing component is arranged on the separation cylinder. The opening and closing component is used to open or close when a set time is reached. A lifting component is also arranged in the ultraviolet irradiation box, and the lifting component includes a traction member and a sealing plate. An intermittent driving component is arranged on the lifting component, and the intermittent driving component is used to raise the sealing plate to a set height through the traction member every time a set time is reached. At this time, the separation cylinder is in an open state, so that the top layer of microorganism inhibition composition is inactivated by the ultraviolet lamp and then enters the separation cylinder, and this is repeated to complete the work of uniformly receiving ultraviolet radiation from all microorganism inhibition compositions.

[0007] Furthermore, the UV irradiation box includes a plurality of evenly arranged UV lamps arranged at the bottom of the UV irradiation box, and the lifting component also includes a first rotating shaft, and a plurality of rope winding rollers are fixedly sleeved on the outer end of the first rotating shaft, one end of the traction member is fixedly wound around the outer wall of the rope winding roller, and the other end is fixedly connected to the top of the sealing plate, a first bevel gear is fixedly sleeved on the first rotating shaft, and the top of the UV irradiation box is rotatably connected to a second rotating shaft, and a second bevel gear is fixedly sleeved on the outer end of the second rotating shaft, and the first bevel gear is meshed with the second bevel gear, and the traction member can lift the sealing plate by driving the second bevel gear to rotate.

[0008] Furthermore, a stirring component is provided inside the UV irradiation box, and the stirring component includes a reduction motor arranged on the top of the UV irradiation box, the output end of the reduction motor is fixedly connected to the driving shaft, the outer end of the driving shaft is fixedly sleeved with a first transmission gear, the inner wall of the UV irradiation box is rotatably connected to a third rotating shaft, the outer end of the third rotating shaft is rotatably sleeved with a rotating sleeve, the outer wall of the rotating sleeve is fixedly connected to a plurality of stirring blades, the outer wall of the rotating sleeve is fixedly sleeved with a second transmission gear, and the first transmission gear is meshed with the second transmission gear.

[0009] Furthermore, a reciprocating pushing component is provided on the stirring component, and the reciprocating pushing component includes an air storage box fixedly connected to the bottom of the ultraviolet irradiation box, a first push plate is slidably connected to the inside of the air storage box, a plurality of push rods are fixedly connected to the side end of the first push plate, an air intake pipe is slidably connected to the inner wall of the air storage box, an exhaust box is fixedly connected to the bottom of the air storage box, a second push plate is slidably connected to the inside of the exhaust box, a movable plate is fixedly connected to the side end of the second push plate, a touch rod is fixedly connected to the outer end of the rotating sleeve, one end of the air intake pipe is connected to the interior of the exhaust box, a rotating plate is fixedly sleeved on the outer end of the third rotating shaft, a plurality of telescopic tubes are fixedly connected to the bottom of the rotating plate, and a plurality of fitting holes are provided on the top of the rotating sleeve.

[0010] Furthermore, a first reset member is fixedly connected between the first push plate and the air storage box, a second reset member is fixedly connected between the second push plate and the exhaust box, a third reset member is fixedly connected between the rotating plate and the outer end of the air storage box, a connecting rod is fixedly connected to the top of the first push plate, a first inclined block is fixedly connected to the end of the connecting rod, the air intake pipe includes a first air pipe and a second air pipe movable inside the first air pipe, a second inclined block is fixedly connected to the bottom of the second air pipe, and an air leakage hole is opened on the second air pipe.

[0011] Furthermore, the opening and closing component includes a fixed plate fixedly connected to the inner wall of the separation cylinder near the top, the bottom of the fixed plate is rotatably connected to a blocking plate, and the top of the blocking plate is fixedly connected to the bottom of the third rotating shaft.

[0012] Furthermore, the intermittent drive component includes a driving disk and a stop disk fixedly sleeved on the third rotating shaft, the top of the ultraviolet irradiation box is rotatably connected to the fourth rotating shaft, the outer end of the fourth rotating shaft is fixedly sleeved with an intermittent disk, the top of the driving disk is fixedly connected with a shift rod, the outer ends of the fourth rotating shaft and the second rotating shaft are both fixedly sleeved with pulleys, and the outer sides of a pair of the pulleys are commonly connected with a transmission belt.

[0013] Furthermore, a baffle plate is fixedly connected to the outer wall of the separation cylinder, a plurality of liquid inlet holes are formed on the baffle plate, and a one-way valve is disposed on the inner wall of the liquid inlet hole.

[0014] A method for preparing a microorganism inhibition composition and application of the prepared microorganism inhibition composition in anti-infection drugs.

[0015] In the above technical scheme, the preparation method of the microorganism inhibitory composition provided by the present invention has the beneficial effects of: In this case, inactivated bacteria are used as antibiotic carriers, and the rapid and short-term antibacterial effect of antibiotics is used to synergistically inactivate microorganisms and stimulate long-term antibacterial immune response, thereby achieving the purpose of efficient and lasting antibacterial. By setting a lifting component and an intermittent driving component, the sealing plate rises to a set height through a traction component every time a set time is reached. At this time, the separation cylinder is in an open state, so that the top layer of microbial carriers is inactivated by ultraviolet light and enters the separation cylinder, while the microorganisms located below can rise again and get closer to the ultraviolet light, and this process is repeated, thereby completing the work of evenly receiving ultraviolet radiation from all microbial carriers, effectively avoiding the microorganisms at the bottom receiving too little ultraviolet light radiation and poor inactivation effect.

[0016] It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the disclosure.

[0017] This application document provides an overview of various implementations or examples of the technology described in the present disclosure, and is not a comprehensive disclosure of the entire scope or all features of the disclosed technology. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in the present invention. For ordinary technicians in this field, other drawings can also be obtained based on these drawings.

[0019] Figure 1 A schematic diagram of the overall external structure provided by an embodiment of the present invention; Figure 2 A schematic diagram of the overall internal structure provided by an embodiment of the present invention; Figure 3 A schematic diagram of the structure of a lifting component provided in an embodiment of the present invention; Figure 4 A schematic diagram of the structure of a stirring component provided in an embodiment of the present invention; Figure 5 A schematic diagram of the external structure of a reciprocating driving component provided in an embodiment of the present invention; Figure 6 A schematic diagram of a half-section structure of a reciprocating driving component provided in an embodiment of the present invention; Figure 7 The embodiment of the present invention provides Figure 6 A schematic diagram of the enlarged structure at point A; Figure 8 A schematic diagram of the structure of the opening and closing components provided in an embodiment of the present invention; Fig. 9 The embodiment of the present invention provides Figure 3 A schematic diagram of the enlarged structure at B; Fig.10 A schematic diagram of the structure of an intermittent drive component provided by an embodiment of the present invention; Fig.11 A schematic diagram of the recovery mechanism structure provided in an embodiment of the present invention.

[0020] Description of reference numerals: 1. UV irradiation box; 2. Separation cylinder; 3. Opening and closing components; 31. Fixed plate; 32. Blocking plate; 33. Drain hole; 4. UV lamp; 5. Lifting component; 51. Traction member; 52. Sealing plate; 53. First rotating shaft; 54. Rope roller; 55. First bevel gear; 56. Second rotating shaft; 57. Second bevel gear; 58. Support frame; 6. Stirring component; 61. Speed ​​reduction motor; 62. Drive shaft; 63. First transmission gear; 64. Third rotating shaft; 65. Rotating sleeve; 66. Stirring blade; 67. Second transmission gear; 7. Reciprocating pushing component; 71. Air storage box; 72. First push plate; 73. Push rod; 74. Inlet pipe; 741. First air Tube; 742, second air pipe; 75, exhaust box; 76, second push plate; 77, movable plate; 78, touch rod; 79, rotating plate; 710, telescopic tube; 711, fitting hole; 712, connecting rod; 713, first inclined block; 714, second inclined block; 715, air leakage hole; 716, first reset member; 8, intermittent drive component; 81, drive disk; 82, anti-rotation disk; 83, fourth rotating shaft; 84, intermittent disk; 85, lever; 86, pulley; 87, transmission belt; 88, arc groove; 89, rectangular groove; 810, accommodating groove; 9, baffle plate; 10, liquid inlet hole; 11, friction sleeve; 12, friction belt; 13, pressing frame; 14, connecting plate. DETAILED DESCRIPTION

[0021] In order to make the purpose, technical solution and advantages of the embodiments of the present disclosure clearer, the technical solution of the embodiments of the present disclosure will be clearly and completely described below in conjunction with the drawings of the embodiments of the present disclosure. Obviously, the described embodiments are part of the embodiments of the present disclosure, not all of the embodiments. Based on the described embodiments of the present disclosure, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present disclosure.

[0022] A microbial inhibition composition comprises a microbial carrier, wherein the microbial carrier is one of Escherichia coli and Staphylococcus aureus, accounting for 70-90% by volume, specifically 80%, and having an optimal shape of a sphere; antibiotic drugs, wherein the antibiotic drugs are loaded on the microbial carrier to exert an inhibitory effect on the microbial carrier; and a calcium-containing solution, a calcium ion solution, such as a calcium chloride solution.

[0023] Raw material preparation; S1, mixing the microbial carrier suspension and the calcium-containing solution evenly through a mixing instrument to obtain a microbial carrier calcium overload mixed solution; S2, incubating the above-mentioned microbial carrier calcium overload mixed solution in an ice bath (temperature of 1-5 degrees Celsius) for 25-35 minutes to obtain a microbial carrier treated with calcium overload; S3, adding an inhibitory drug to the microbial carrier treated with calcium overload to obtain a drug-loaded microbial carrier; S4, placing the drug-loaded microbial carrier into an ultraviolet irradiation box and irradiating it with ultraviolet light for inactivation; See also Figure 1-3 A separation cylinder 2 is fixedly connected to the inner bottom of the ultraviolet irradiation box 1, and an opening and closing component 3 is provided on the separation cylinder 2. The opening and closing component 3 is used to open or close when the set time is reached. A lifting component 5 is also provided in the ultraviolet irradiation box 1, and the lifting component 5 includes a traction member 51 and a sealing plate 52. An intermittent driving component 8 is provided on the lifting component 5. The intermittent driving component 8 is used to rise to a set height with the sealing plate 52 through the traction member 51 every time the set time is reached. At this time, the separation cylinder 2 is in an open state, so that the top layer of microorganism carriers are inactivated by the ultraviolet lamp 4 and then enter the separation cylinder 2. This is repeated to complete the work of all microorganism carriers being evenly irradiated by the ultraviolet lamp 4.

[0024] Since the irradiation range of ultraviolet light is limited in a short time, the microorganism inhibition composition located at the bottom layer can be lifted to a high place by the lifting component 5, close to the ultraviolet lamp 4, thereby improving the ultraviolet irradiation effect there, so that all microorganism inhibition compositions can be evenly irradiated by the ultraviolet lamp 4.

[0025] See also Figure 3 , Fig. 9 In an embodiment further provided by the present invention, the UV irradiation box 1 includes a plurality of UV lamps 4 arranged evenly at the bottom of the UV irradiation box 1, and the lifting component 5 also includes a first rotating shaft 53, and the outer end of the first rotating shaft 53 is fixedly sleeved with a plurality of rope winding rollers 54, one end of the traction member 51 is fixedly wound around the outer wall of the rope winding roller 54, and the other end is fixedly connected to the top of the sealing plate 52, and a first bevel gear 55 is fixedly sleeved on the first rotating shaft 53, and the top of the UV irradiation box 1 is rotatably connected with a second rotating shaft 56, and the outer end of the second rotating shaft 56 is fixedly sleeved with a second bevel gear 57, and the first bevel gear 55 is meshed with the second bevel gear 57, and by driving the second bevel gear 57 to rotate, the traction member 51 can lift the sealing plate 52.

[0026] Specifically, the top of the UV irradiation box 1 is detachably connected to four support frames 58, and an axial hole is opened on the support frame 58. A pair of first rotating shafts 53 are arranged, and both are movable through the inside of the axial hole. The traction member 51 is a traction rope and is sprayed with an anti-sticking layer. The UV lamp 4 near the middle is arranged at an angle, so that the UV area can cover the entire area. The outside of a pair of first rotating shafts 53 are fixedly sleeved with friction sleeves 11, and a pair of friction sleeves 11 are provided with friction belts 12 on the outside. The friction sleeves 11 and the friction belts 12 are frictionally connected, and a sealing sleeve is provided between the sealing plate 52 and the inner wall of the UV irradiation box 1.

[0027] When the second bevel gear 57 rotates, it drives the first bevel gear 55 to rotate, causing the first rotating shaft 53 to start rotating. Due to the transmission connection between the friction sleeve 11 and the friction belt 12, the pair of first rotating shafts 53 both start to rotate with the rope roller 54, and the sealing plate 52 is pulled up through the traction member 51.

[0028] See also Figure 4 In an embodiment further provided by the present invention, a stirring component 6 is arranged inside the ultraviolet irradiation box 1, and the stirring component 6 includes a reduction motor 61 arranged on the top of the ultraviolet irradiation box 1, and the output end of the reduction motor 61 is fixedly connected to a driving shaft 62, and the outer end of the driving shaft 62 is fixedly sleeved with a first transmission gear 63, and the inner wall of the ultraviolet irradiation box 1 is rotatably connected to a third rotating shaft 64, and the outer end of the third rotating shaft 64 is rotatably sleeved with a rotating sleeve 65, and the outer wall of the rotating sleeve 65 is fixedly connected with a plurality of stirring blades 66, and the specific number can be three, and the outer wall of the rotating sleeve 65 is fixedly sleeved with a second transmission gear 67, and the first transmission gear 63 is meshed with the second transmission gear 67.

[0029] Specifically, by turning on the reduction motor 61, the reduction motor 61 drives the first transmission gear 63 to rotate, so that the first transmission gear 63 drives the second transmission gear 67 to rotate. Since the second transmission gear 67 is fixedly connected to the rotating sleeve 65, the rotating sleeve 65 drives the three stirring blades 66 to rotate slowly and continuously, so that a pile of top layer solutions can be stirred, so that each layer of solution above the separation cylinder 2 can reach the top layer, thereby further improving the full contact between the microorganism inhibition composition and the ultraviolet light.

[0030] See also Figure 5-7 In the embodiment provided by the present invention, a reciprocating pushing component 7 is provided on the stirring component 6, and the reciprocating pushing component 7 includes an air storage box 71 fixedly connected to the bottom of the ultraviolet irradiation box 1, a first push plate 72 is slidably connected inside the air storage box 71, and a plurality of push rods 73 are fixedly connected to the side end of the first push plate 72, an air inlet pipe 74 is slidably connected to the inner wall of the air storage box 71, an exhaust box 75 is fixedly connected to the bottom of the air storage box 71, a second push plate 76 is slidably connected inside the exhaust box 75, and a movable plate 77 is fixedly connected to the side end of the second push plate 76, and the movable plate 77 is T-shaped and movably penetrates to the outside of the exhaust box 75, a touch rod 78 is fixedly connected to the outer end of the rotating sleeve 65, one end of the air inlet pipe 74 is connected to the inside of the exhaust box 75, a rotating plate 79 is fixedly sleeved on the outer end of the third rotating shaft 64, a plurality of telescopic tubes 710 are fixedly connected to the bottom of the rotating plate 79, and a plurality of fitting holes 711 are opened on the top of the rotating sleeve 65.

[0031] Specifically, an air inlet hole is opened at the front end of the exhaust box 75, and a one-way valve is provided inside the air inlet hole and the air inlet pipe 74, and a telescopic spring is provided inside the telescopic tube 710.

[0032] A first reset member 716 is fixedly connected between the first push plate 72 and the air storage box 71, a second reset member is fixedly connected between the second push plate 76 and the exhaust box 75, a third reset member is fixedly connected between the rotating plate 79 and the outer end of the air storage box 71, a connecting rod 712 is fixedly connected to the top of the first push plate 72, a first inclined block 713 is fixedly connected to the end of the connecting rod 712, the air intake pipe 74 includes a first air pipe 741 and a second air pipe 742 movable inside the first air pipe 741, a second inclined block 714 is fixedly connected to the bottom of the second air pipe 742, a leakage hole 715 is opened on the second air pipe 742, and a third reset member is fixedly connected between the first inclined block 713 and the inner wall of the air storage box 71.

[0033] Specifically, the first return member 716 , the second return member and the third return member are all return springs.

[0034] See also Figure 8 In the solution further provided by the present invention, the opening and closing component 3 includes a fixed plate 31 fixedly connected to the inner wall of the separation cylinder 2 near the top, the bottom of the fixed plate 31 is rotatably connected with a sealing plate 32, the top of the sealing plate 32 is fixedly connected to the bottom of the third rotating shaft 64, a drainage hole 33 is opened on the fixed plate 31, and the cross-sectional area of ​​the drainage hole 33 is smaller than the cross-sectional area of ​​the sealing plate 32, and the bottom of the ultraviolet irradiation box 1 is fixedly connected with a drainage pipe, and a control valve is arranged inside the drainage pipe.

[0035] The reduction motor 61 is turned on to drive the rotating sleeve 65 to rotate. During the rotation process, the rotating sleeve 65 will also squeeze the movable plate 77 with the touch rod 78 for each rotation. The movable plate 77 moves with the second push plate 76. During the movement of the second push plate 76, the internal gas of the exhaust box 75 is transported to the inside of the air storage box 71 through the air inlet pipe 74, so that the air pressure above the first push plate 72 increases. After a period of time (generally 30-60 seconds), the first push plate 72 brings the push rod 73 to push the rotating plate 79 down, so that the telescopic tube 710 is combined with the leakage hole 715, thereby driving the third rotating shaft 64 to rotate, forcing the sealing plate 32 to start rotating, so that the top of the separation cylinder 2 is open. According to the distance between the communicating vessel and the connecting vessel, the liquid will automatically fall into the inside of the separation cylinder 2. During this period, the telescopic tube 710 is always in a contracted state. When the third rotating shaft 64 rotates just one circle, the third An inclined block 713 pushes the second inclined block 714 to move horizontally until a partial area of ​​the second air pipe 742 is pushed to the outside of the air storage box 71, exposing the leakage hole 715 to the outside. At this time, the air pressure inside the air storage box 71 begins to decrease, and the gas leaks to the outside of the air storage box 71 through the second air pipe 742 and the leakage hole 715 in succession, so that the first push plate 72 returns to the initial position under the action of the first reset member 716. After losing the squeezing of the first inclined block 713, the second inclined block 714 also returns to the initial position under the action of the third reset member. At this time, the leakage hole 715 returns to the interior of the air storage box 71, and the gas from the exhaust box 75 can enter the interior of the air storage box 71 through the first air pipe 741 and the second air pipe 742, pushing the first push plate 72 to move again, and so on and so forth, thereby driving the blocking plate 32 to rotate one circle at regular intervals.

[0036] See also Fig.10 In the solution further provided by the present invention, the intermittent drive component 8 includes a driving disk 81 and a stop disk 82 fixedly sleeved on the third rotating shaft 64, the top of the ultraviolet irradiation box 1 is rotatably connected to the fourth rotating shaft 83, the outer end of the fourth rotating shaft 83 is fixedly sleeved with the intermittent disk 84, the top of the driving disk 81 is fixedly connected with a lever 85, the outer end of the fourth rotating shaft 83 and the outer end of the second rotating shaft 56 are both fixedly sleeved with a pulley 86, and the outer sides of the pair of pulleys 86 are commonly connected with a transmission belt 87 for transmission.

[0037] Specifically, a plurality of arcuate grooves 88 and rectangular grooves 89 are provided on the intermittent disk 84 , and a receiving groove 810 is provided on the anti-rotation disk 82 . The embodiment provided in this case has six arcuate grooves 88 and rectangular grooves 89 .

[0038] When the blocking plate 32 starts to rotate to the first angle (45-90 degrees), the drainage hole 33 is fully opened, and the microorganism inhibitory composition located in the upper layer continues to be in the "draining" state. Then the blocking plate 32 continues to rotate and is still in the "draining" state. When the blocking plate 32 rotates to the second angle (270-330 degrees), the blocking plate 32 blocks the drainage hole 33. At this time, the upper liquid is discharged. At the same time, the lever 85 on the driving disk 81 begins to enter the rectangular groove 89 and pushes the intermittent disk 84 to rotate. When the blocking plate 32 rotates 360 degrees, it can just push the intermittent disk 84 to rotate. The fourth rotating shaft 83 and the second rotating shaft 56 are synchronously rotated 60 degrees through the pulley 86 and the transmission belt 87, and finally the four rope winding rollers 54 are all rotated 60 degrees, so that the four traction members 51 simultaneously pull the sealing plate 52 up a short distance, and can also carry part of the microorganism inhibition composition for ultraviolet irradiation treatment. This is repeated, so that all the microorganism inhibition compositions can be uniformly irradiated with ultraviolet light, effectively avoiding that the microorganism inhibition composition in the bottom area is insufficiently irradiated with ultraviolet light, and the microorganism inhibition composition in the top layer is in contact for too long and irradiated excessively, thereby killing the microorganisms.

[0039] In a solution further provided by the present invention, a baffle plate 9 is fixedly connected to the outer wall of the separation cylinder 2 , a plurality of liquid inlet holes 10 are formed on the baffle plate 9 , and a one-way valve is disposed on the inner wall of the liquid inlet hole 10 .

[0040] In order to further avoid excessive irradiation of the microorganism inhibitory composition, a baffle plate 9 may be provided, the baffle plate 9 being sprayed with an anti-ultraviolet coating, the microorganism inhibitory composition enters the top of the baffle plate 9 through the liquid inlet hole 10, and begins to receive the radiation of the ultraviolet lamp 4, while the microorganism inhibitory composition located below the baffle plate 9 is hardly irradiated by the ultraviolet lamp 4, thereby further ensuring that each part of the radiation area of ​​the ultraviolet lamp 4 can evenly receive the radiation of the ultraviolet lamp 4.

[0041] See also Fig.11 In this case, a restoration mechanism is also provided, which includes a pressing frame 13, and a pair of connecting plates 14 are fixedly connected to the end of the pressing frame 13. The pair of connecting plates 14 are fixedly connected to the rotating plate 79. By touching the pressing frame 13, the rotating plate 79 can be lowered through the connecting plates 14, so that the telescopic tube 710 enters the inside of the fitting hole 711, and then the reduction motor 61 is reversed to reverse the first rotating shaft 53 with the rope roller 54. When the pressing frame 13 is released, the rotating plate 79 can return to the initial position under the action of the third reset member.

[0042] Optionally, the first bevel gear 55 and the second bevel gear 57 can be no longer meshed by lifting four brackets or a pair of first rotating shafts 53, and then the sealing plate 52 automatically falls to the initial position under the action of gravity. This method is simpler and more convenient.

[0043] In the present invention, the microorganism inhibitory composition prepared by the preparation method of the microorganism inhibitory composition can be used in anti-infection drugs.

[0044] The above description is only by way of illustration of certain exemplary embodiments of the present invention. It is undoubted that those skilled in the art can modify the described embodiments in various ways without departing from the spirit and scope of the present invention. Therefore, the above drawings and descriptions are illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present invention.

Claims

1. A microorganism inhibition composition, characterized in that: The microbial carrier comprises one of Escherichia coli and Staphylococcus aureus, accounting for 70-90% by volume; Antibiotic drugs, which are used to be loaded on microbial carriers to inhibit them; Calcium solution.

2. A method for preparing a microorganism inhibitory composition, the microorganism inhibitory composition according to claim 1, characterized in that: Raw material preparation; The microbial carrier suspension and the calcium-containing solution are mixed evenly by a mixing instrument to obtain a microbial carrier calcium overload mixed solution; The microbial carrier calcium overload mixed solution is co-incubated in an ice bath for 25-35 minutes to obtain a calcium overloaded microbial carrier; Adding an inhibitory drug to the calcium overloaded microbial carrier to obtain a drug-loaded microbial carrier; The drug-loaded microbial carrier is placed in an ultraviolet irradiation box (1) and irradiated with an ultraviolet lamp (4) to perform an inactivation treatment; The inner bottom of the ultraviolet irradiation box (1) is fixedly connected to a separation cylinder (2), and an opening and closing component (3) is arranged on the separation cylinder (2), and the opening and closing component (3) is used to open or close when a set time is reached. The ultraviolet irradiation box (1) is also provided with a lifting component (5), and the lifting component (5) includes a traction component (51) and a sealing plate (52). An intermittent driving component (8) is arranged on the lifting component (5), and the intermittent driving component (8) is used to lift the sealing plate (52) to a set height through the traction component (51) every time a set time is reached. At this time, the separation cylinder (2) is in an open state, so that the top microorganism inhibition composition is inactivated by the ultraviolet lamp (4) and then enters the separation cylinder (2), and this is repeated to complete the work of uniformly receiving the radiation of the ultraviolet lamp (4) on all the microorganism inhibition compositions.

3. The method for preparing the microorganism inhibitory composition according to claim 2, characterized in that: The ultraviolet irradiation box (1) comprises a plurality of uniformly arranged ultraviolet lamps (4) arranged at the bottom of the ultraviolet irradiation box (1); the lifting component (5) further comprises a first rotating shaft (53); a plurality of rope winding rollers (54) are fixedly sleeved on the outer end of the first rotating shaft (53); one end of the traction member (51) is fixedly wound around the outer wall of the rope winding roller (54); the other end is fixedly connected to the top of the sealing plate (52); a first bevel gear (55) is fixedly sleeved on the first rotating shaft (53); a second rotating shaft (56) is rotatably connected to the top of the ultraviolet irradiation box (1); a second bevel gear (57) is fixedly sleeved on the outer end of the second rotating shaft (56); the first bevel gear (55) is meshed with the second bevel gear (57); and by driving the second bevel gear (57) to rotate, the traction member (51) can lift the sealing plate (52).

4. The method for preparing the microorganism inhibitory composition according to claim 3, characterized in that: A stirring component (6) is arranged inside the ultraviolet irradiation box (1), and the stirring component (6) comprises a reduction motor (61) arranged on the top of the ultraviolet irradiation box (1); the output end of the reduction motor (61) is fixedly connected to a drive shaft (62); the outer end of the drive shaft (62) is fixedly sleeved with a first transmission gear (63); the inner wall of the ultraviolet irradiation box (1) is rotatably connected to a third rotating shaft (64); the outer end of the third rotating shaft (64) is rotatably sleeved with a rotating sleeve (65); the outer wall of the rotating sleeve (65) is fixedly connected to a plurality of stirring blades (66); the outer wall of the rotating sleeve (65) is fixedly sleeved with a second transmission gear (67); the first transmission gear (63) is meshed with the second transmission gear (67).

5. The method for preparing the microorganism inhibitory composition according to claim 4, characterized in that: The stirring component (6) is provided with a reciprocating pushing component (7), the reciprocating pushing component (7) comprising an air storage box (71) fixedly connected to the bottom of the ultraviolet irradiation box (1), a first push plate (72) being slidably connected to the inside of the air storage box (71), a plurality of push rods (73) being fixedly connected to the side end of the first push plate (72), an air inlet pipe (74) being slidably connected to the inner wall of the air storage box (71), an exhaust box (75) being fixedly connected to the bottom of the air storage box (71), and the exhaust box (75) ) is slidably connected to the inside of the second push plate (76), the side end of the second push plate (76) is fixedly connected to a movable plate (77), the outer end of the rotating sleeve (65) is fixedly connected to a touch rod (78), one end of the intake pipe (74) is connected to the inside of the exhaust box (75), the outer end of the third rotating shaft (64) is fixedly sleeved with a rotating plate (79), the bottom of the rotating plate (79) is fixedly connected to a plurality of telescopic tubes (710), and the top of the rotating sleeve (65) is provided with a plurality of fitting holes (711).

6. The method for preparing the microorganism inhibitory composition according to claim 5, characterized in that: A first restoring member (716) is fixedly connected between the first push plate (72) and the air storage box (71), a second restoring member is fixedly connected between the second push plate (76) and the exhaust box (75), a third restoring member is fixedly connected between the rotating plate (79) and the outer end of the air storage box (71), a connecting rod (712) is fixedly connected to the top of the first push plate (72), a first inclined block (713) is fixedly connected to the end of the connecting rod (712), the air intake pipe (74) comprises a first air pipe (741) and a second air pipe (742) movable inside the first air pipe (741), a second inclined block (714) is fixedly connected to the bottom of the second air pipe (742), and an air leakage hole (715) is provided on the second air pipe (742).

7. The method for preparing the microorganism inhibitory composition according to claim 6, characterized in that: The opening and closing component (3) comprises a fixed plate (31) fixedly connected to the inner wall of the separation cylinder (2) near the top, the bottom of the fixed plate (31) being rotatably connected to a blocking plate (32), and the top of the blocking plate (32) being fixedly connected to the bottom of the third rotating shaft (64).

8. The method for preparing the microorganism inhibitory composition according to claim 7, characterized in that: The intermittent drive component (8) comprises a driving disk (81) and a stop disk (82) fixedly sleeved on the third rotating shaft (64); the top of the ultraviolet irradiation box (1) is rotatably connected to the fourth rotating shaft (83); the outer end of the fourth rotating shaft (83) is fixedly sleeved with the intermittent disk (84); the top of the driving disk (81) is fixedly connected to a shifting rod (85); the outer ends of the fourth rotating shaft (83) and the second rotating shaft (56) are both fixedly sleeved with pulleys (86); and the outer ends of the pair of pulleys (86) are commonly connected to a transmission belt (87) for transmission.

9. The method for preparing the microorganism inhibitory composition according to claim 8, characterized in that: A baffle plate (9) is fixedly connected to the outer wall of the separation cylinder (2), a plurality of liquid inlet holes (10) are provided on the baffle plate (9), and a one-way valve is provided on the inner wall of the liquid inlet hole (10).

10. Use of the microorganism inhibitory composition prepared by the method for preparing the microorganism inhibitory composition according to any one of claims 2 to 9 in anti-infective drugs.

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