Collagen microsphere production-based endotoxin removal device and use method thereof

Abstract

The invention discloses an endotoxin removing device based on collagen microsphere production and a using method thereof, and relates to the field of collagen microsphere production, the technical scheme is that the endotoxin removing device comprises a base, a filtering tank and a treatment tank, the filtering tank and the treatment tank are located on the base, and a reciprocating pipeline assembly is installed between the filtering tank and the treatment tank; a collagen microsphere solution can be transported between the two tank bodies in a reciprocating manner, so that the collagen microsphere solution entering and exiting from the treatment tank can be filtered by the filtering tank; a liquid inlet pipe is mounted at the upper part of the outer side wall of the filtering tank, a liquid discharge pipe is mounted at the lower part of the outer side wall of the filtering tank, and a filtering plate is mounted in the middle of the filtering tank; and an endotoxin adsorption column is mounted in the filtering tank, so that two times of filtering can be completed by only one filtering tank in the whole process, the cost is saved, the size of the device is reduced, and the device is very beneficial to being used in small factories.

Description

Technical Field

[0001] The present invention relates to the field of collagen microsphere production, and more specifically, to an endotoxin removal device based on collagen microsphere production and a method for using the same. Background Art

[0002] The removal of endotoxins is a crucial step in the production process of collagen microspheres. This is mainly because endotoxins, as the lipopolysaccharide component of the outer layer of the cell wall of Gram-negative bacteria, are highly toxic even in trace amounts. Once collagen microspheres are used in medical and other related applications, trace amounts of endotoxins may cause serious adverse reactions to the human body or other organisms, such as fever, inflammatory response, and immune regulation disorders. Therefore, the endotoxin content must be reduced to below the safety standard to ensure that the final collagen microsphere product meets the strict requirements for endotoxin content and ensures its safe application in biomedicine and other fields.

[0003] In the whole process of collagen microspheres treating endotoxins, the filtration link plays an important role. Filtration before treatment is mainly to remove large particle impurities that may exist in the raw material solution, such as incompletely dissolved solid matter, some foreign particles brought in by the raw materials, etc. If these large particle impurities are not removed in advance, they may clog key treatment equipment such as endotoxin adsorption columns when entering the subsequent endotoxin treatment link, affecting the efficiency and effect of endotoxin removal, and may even damage the equipment. Therefore, filtration before treatment is a necessary step to ensure the smooth progress of subsequent treatment processes. Filtration after treatment is because after the endotoxin treatment process, such as when using endotoxin adsorption columns for treatment, some new fine impurities may be generated, such as detached particles of adsorption materials, and some complex residues that may be formed after the action of endotoxins and adsorption materials. If these fine impurities remain in the collagen microsphere solution, they will affect the quality and purity of the product, so they need to be removed by filtration again to ensure that the final collagen microsphere product meets the relevant quality standards.

[0004] However, this practice of setting up filter tanks before and after treatment undoubtedly brings many disadvantages. On the one hand, the purchase of two filter tanks increases the equipment procurement cost. For small factories with relatively limited funds, this is undoubtedly a considerable expense; on the other hand, the two filter tanks need to occupy a certain space to be placed. Small factories often have relatively cramped space, which makes the already tight space even more crowded, which is not conducive to the reasonable layout and efficient operation of the factory, and brings great troubles to small factories in the production of collagen microspheres and endotoxin treatment.

[0005] Therefore, in order to solve the above technical problems, the present application proposes an endotoxin removal device based on collagen microsphere production and a method of using the same. Summary of the invention

[0006] In view of the shortcomings of the prior art, the object of the present invention is to provide an endotoxin removal device based on collagen microsphere production and a method of using the same.

[0007] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: an endotoxin removal device based on collagen microsphere production, comprising a base and a filter tank and a treatment tank located on the base, wherein a reciprocating pipeline assembly is installed between the filter tank and the treatment tank, and the collagen microsphere solution can be reciprocated between the two tanks, so that the collagen microsphere solution entering and exiting the treatment tank can be filtered by the filter tank; A liquid inlet pipe is installed at the upper part of the outer wall of the filter tank, a liquid discharge pipe is installed at the lower part of the outer wall, and a filter plate is installed in the middle of the filter tank; An endotoxin adsorption column is installed inside the filter tank.

[0008] Preferably, the reciprocating pipeline assembly includes a two-way water pump, one end of which is connected to a connecting pipe A and a connecting pipe B through an electric three-way ball valve A, the connecting pipe A is communicated with an upper portion of the interior of the filter tank, and the connecting pipe B is communicated with a lower portion of the interior of the filter tank; The other end of the two-way water pump is connected to the connecting pipe C and the connecting pipe D through the electric three-way ball valve B. The connecting pipe C is connected to the upper part of the interior of the treatment tank, and the connecting pipe D is connected to the lower part of the interior of the treatment tank.

[0009] Preferably, electric valves are installed on the connecting pipe A, the connecting pipe B, the connecting pipe C, the connecting pipe D, the liquid inlet pipe and the liquid discharge pipe.

[0010] Preferably, a top cover A is installed at the top of the filter tank, and the bottom of the top cover A is connected to the filter plate through vertical rods. A stirring paddle A driven by a motor A is installed at the bottom of the top cover A, and the stirring paddle A is located directly above the filter plate.

[0011] Preferably, the bottom end of the vertical rod is fixedly connected with a plug-in block, the interior of the filter plate is embedded with a plurality of plug-in slots matched with the plug-in block, the bottom end of the plug-in block is fixedly connected with a screw, the bottom of the plug-in slot is provided with a through hole for the screw to pass through, and a nut is threadedly connected on the outer wall of the screw.

[0012] Preferably, the endotoxin adsorption column is in the shape of a cylindrical groove, a top cover B is installed at the top of the treatment tank, and a stirring paddle B driven by a motor B is installed at the bottom of the top cover B. The stirring paddle B is placed in the endotoxin adsorption column to promote the flow of solution in the endotoxin adsorption column.

[0013] Preferably, a plurality of infusion pumps are distributed in a circumferential pattern on the inner wall of the treatment tank, and the infusion pump is provided with a connecting tube for inputting the solution at the bottom of the treatment tank to the top of the endotoxin adsorption column, so as to promote the circulation of the solution.

[0014] Preferably, the top of the base is connected to the controller via a bracket, and the design of the bracket facilitates the staff to operate the controller.

[0015] Preferably, forklift slots are provided on both sides of the front side ends of the base, and universal wheels are installed around the bottom of the base to facilitate its movement, and the universal wheels make it easy for staff to push the device.

[0016] The method of using the above-mentioned endotoxin removal device produced based on collagen microspheres comprises the following steps: Step 1: Connect the collagen microsphere solution infusion pipeline to the liquid inlet pipe on the filter tank, so as to input the solution into the filter tank; Step 2: Switch the position of the valve core of the electric three-way ball valve A through the controller, connect the connecting pipe B to the two-way water pump, and switch the position of the valve core of the electric three-way ball valve B at the same time, connect the connecting pipe C to the electric two-way water pump, input the solution from the filter tank to the treatment tank through the electric two-way water pump, and the endotoxin adsorption column in the treatment tank adsorbs the endotoxin in the solution; Step 3: After the adsorption is completed, the position of the valve core of the electric three-way ball valve A is switched through the controller, and the connecting pipe A is connected to the two-way water pump. At the same time, the position of the valve core of the electric three-way ball valve B is switched, and the connecting pipe D is connected to the electric two-way water pump. Then, the filtered collagen microsphere solution is input from the bottom of the treatment tank to the top of the filter tank through the electric two-way water pump to be filtered again through the filter plate. Step 4: Open the electric valve on the drain pipe, and the filtered solution is discharged from the filter tank through the drain pipe.

[0017] Compared with the prior art, the present invention has the following beneficial effects: 1. The present invention filters the collagen microsphere solution by a filter tank to remove large particles of impurities. After the filtration, the solution is input into a treatment tank through a reciprocating pipe assembly, and the endotoxin adsorption column in the treatment tank adsorbs the endotoxin in the solution. Then, the solution is input into the filter tank by the reciprocating pipe assembly to filter the impurities generated during the treatment process. After the filtration is completed, the solution is discharged from the drain pipe. In this way, only one filter tank is needed to complete two filtrations in the whole process, which saves costs and reduces the size of the device, and is very beneficial to the use of small factories, thereby solving the problem that the background technology is not conducive to the use of small factories. 2. In the present invention, the motor A drives the stirring paddle A to rotate, and the stirring paddle A stirs the solution above the filter plate by rotating to avoid accumulation of impurities, which can effectively avoid clogging of the filter plate; 3. The quick-release structure of the present invention makes it easier to disassemble and assemble the filter plate, making it more convenient for the staff to clean and replace the filter plate; 4. The present invention uses the infusion pump and the circulation pipe to input the solution at the bottom of the treatment tank to the top of the endotoxin adsorption column, thereby entering the cylindrical groove, and then passing through the edge of the endotoxin adsorption column until it is transported by the circulation tank again. Such repeated circulation can promote the flow of the solution in the treatment tank, and can effectively improve the contact effect between the solution and the endotoxin adsorption column, thereby improving the removal effect of endotoxin; 5. The present invention uses the infusion pump and the circulation pipe to input the solution at the bottom of the treatment tank to the top of the endotoxin adsorption column, thereby entering the cylindrical groove, and then passing through the edge of the endotoxin adsorption column until it is transported by the circulation tank again. Such repeated circulation can promote the flow of the solution in the treatment tank, and can effectively improve the contact effect between the solution and the endotoxin adsorption column, thereby improving the removal effect of endotoxin; 6. The forklift slot on the base of the present invention can cooperate with a forklift to move the device, and the universal wheels make it easy for the staff to push the device, thereby providing convenience for the movement of the device. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The drawings described herein are used to provide a further understanding of the present invention and constitute a part of this application. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation of the present invention. In the drawings: Figure 1 It is a schematic diagram of the overall structure of the present invention; Figure 2 It is a schematic diagram of the specific structure of the side part of the present invention; Figure 3 This is a schematic diagram of the connection structure of the top cover A in the present invention; Figure 4 For the present invention Figure 3 A local structure enlarged view; Figure 5 For the present invention Figure 3 A schematic diagram of the specific structure from another angle; Figure 6 For the present invention Figure 5 B is an enlarged view of the local structure; Figure 7 It is a schematic diagram of the specific internal structure of the filter tank of the present invention.

[0019] In the figure: 1. Base; 2. Filter tank; 3. Treatment tank; 4. Reciprocating pipe assembly; 401. Two-way water pump; 402. Electric three-way ball valve A; 403. Connecting pipe A; 404. Connecting pipe B; 405. Electric three-way ball valve B; 406. Connecting pipe C; 407. Connecting pipe D; 5. Liquid inlet pipe; 6. Liquid discharge pipe; 7. Filter plate; 8. Top cover A; 9. Motor A; 10. Stirring paddle A; 11. Plug-in block; 12. Plug-in slot; 13. Screw; 14. Through hole; 15. Nut; 16. Top cover B; 17. Motor B; 18. Stirring paddle B; 19. Infusion pump; 20. Circulation tube; 21. Bracket; 22. Controller; 23. Forklift slot; 24. Universal wheel; 25. Endotoxin adsorption column; 26. Vertical rod. DETAILED DESCRIPTION

[0020] Example 1 like Figure 1 and Figure 2 As shown, the present invention provides an endotoxin removal device based on collagen microsphere production, including a base 1 and a filter tank 2 and a treatment tank 3 located on the base 1, a reciprocating pipe assembly 4 is installed between the filter tank 2 and the treatment tank 3, and the collagen microsphere solution can be reciprocated between the two tanks, so that the collagen microsphere solution entering and exiting the treatment tank 3 can be filtered by the filter tank 2, a liquid inlet pipe 5 is installed at the upper part of the outer wall of the filter tank 2, and a liquid discharge pipe 6 is installed at the lower part of the outer wall, a filter plate 7 is installed in the middle of the filter tank 2, an endotoxin adsorption column 25 is installed inside the filter tank 2, and a heating component is installed in the treatment tank 3 for heating the collagen microsphere solution to an appropriate temperature for removing endotoxins.

[0021] During use, the collagen microsphere solution infusion pipeline is first connected to the liquid inlet pipe 5 on the filter tank 2, so that the solution is input into the filter tank 2, and the collagen microsphere solution is filtered by the filter tank 2 to remove large particles of impurities. The solution after filtering is input into the treatment tank 3 through the reciprocating pipe assembly 4, and the endotoxin adsorption column 25 in the treatment tank 3 adsorbs the endotoxin in the solution, and then the reciprocating pipe assembly 4 inputs it into the filter tank 2 to filter the impurities generated during the treatment process. After the filtration is completed, it is discharged from the drain pipe 6 (the drain pipe 6 is connected to other pipelines for transporting the treated solution to other devices for processing). In this way, only one filter tank 2 is needed to complete two filtrations in the whole process, which saves costs and reduces the size of the device, which is very beneficial for use in small factories.

[0022] Furthermore, forklift grooves 23 are provided on both sides of the front side ends of the base 1, and universal wheels 24 are installed around the bottom of the base 1 to facilitate its movement. The forklift grooves 23 can cooperate with a forklift to move the device, and the universal wheels 24 are convenient for the staff to push the device, thereby facilitating the movement of the device. In addition, the top of the base 1 is connected to the controller 22 through the bracket 21, and the controller 22 is supported by the bracket 21 at a height that is convenient for the staff to operate (without bending over), and the various components of the device are controlled by the controller 22.

[0023] Example 2 like Figure 1 and Figure 2 As shown, this embodiment provides a specific structure of the reciprocating pipe assembly 4 in Embodiment 1: the reciprocating pipe assembly 4 includes a two-way water pump 401, one end of the two-way water pump 401 is connected to a connecting pipe A403 and a connecting pipe B404 through an electric three-way ball valve A402, the connecting pipe A403 is communicated with the upper part of the interior of the filter tank 2, and the connecting pipe B404 is communicated with the lower part of the interior of the filter tank 2; The other end of the two-way water pump 401 is connected to the connecting pipe C406 and the connecting pipe D407 through the electric three-way ball valve B405. The connecting pipe C406 is connected to the upper part of the interior of the processing tank 3, and the connecting pipe D407 is connected to the lower part of the interior of the processing tank 3.

[0024] When in use, the position of the valve core of the electric three-way ball valve A402 is switched through the controller 22, the connecting pipe B404 is connected to the two-way water pump 401, and the position of the valve core of the electric three-way ball valve B405 is switched at the same time, the connecting pipe C406 is connected to the electric two-way water pump 401, and the solution is input from the filter tank 2 to the processing tank 3 through the electric two-way water pump 401. In this way, after the collagen microsphere solution is filtered by the filter plate 7 in the filter tank 2, the filtered solution will be input from the bottom of the filter tank 2 (the solution at the bottom of the filter tank 2 has passed through the filter plate 7 and has been filtered, and the solution is transported from a position close to the bottom to effectively avoid solution residue) to the top of the processing tank 3 (compared to The endotoxin in the solution is adsorbed by the endotoxin adsorption column 25 in the treatment tank 3. After the adsorption is completed, the position of the valve core of the electric three-way ball valve A402 is switched through the controller 22, and the connecting pipe A403 is connected to the two-way water pump 401. At the same time, the position of the valve core of the electric three-way ball valve B405 is switched, and the connecting pipe D407 is connected to the electric two-way water pump 401. After that, the filtered collagen microsphere solution is input from the bottom of the treatment tank 3 to the top of the filter tank 2 through the electric two-way water pump 401, so as to be filtered again through the filter plate 7, and the filtered solution is discharged from the filter tank 2 through the drain pipe 6.

[0025] Furthermore, electric valves are installed on connecting pipe A403, connecting pipe B404, connecting pipe C406, connecting pipe D407, liquid inlet pipe 5 and discharge pipe 6, so that each pipeline can be closed when not in use to avoid backflow, and when a local pipeline is damaged, other pipelines can be closed, and the local pipeline that needs to be disassembled can be isolated from the entire system, which is more convenient for maintenance and replacement. The electric valve on the discharge pipe 6 is even more important. Since the solution needs to be filtered twice, the discharge pipe 6 needs to be closed for a long time by the electric valve to prevent the solution from being discharged before it is completely processed.

[0026] It should be noted that the original term "interior upper portion" in the present invention refers to the portion flush with the top, which is equivalent to the top, and the original term "interior lower portion" refers to the portion flush with the bottom, which is equivalent to the bottom.

[0027] Example 3 The filter plate 7 in Example 1 is prone to clogging and needs to be frequently replaced and cleaned, which seriously increases the workload of the staff. Figure 1 - Figure 6 As shown, the present invention installs a top cover A8 on the top of the filter tank 2, and the bottom of the top cover A8 is connected to the filter plate 7 through vertical rods 26. The bottom of the top cover A8 is installed with a stirring paddle A10 driven by a motor A9, and the stirring paddle A10 is located directly above the filter plate 7.

[0028] In this way, the filter plate 7 is connected to the top cover A8 through the vertical rod 26. When the top cover A8 is disassembled, the filter plate 7 can be removed from the filter tank 2 together with the filter plate 7. In addition, during the daily filtering process of the filter tank 2, the motor A9 can be turned on to drive the stirring paddle A10 to rotate. The stirring paddle A10 stirs the solution above the filter plate 7 by rotating to avoid the accumulation of impurities, which can effectively avoid the clogging of the filter plate 7.

[0029] Furthermore, the bottom end of the vertical rod 26 is fixedly connected to the plug-in block 11, and a plurality of plug-in slots 12 matched with the plug-in block 11 are embedded and fixed inside the filter plate 7. The bottom end of the plug-in block 11 is fixedly connected to the screw 13, and the bottom of the plug-in slot 12 is provided with a through hole 14 for the screw 13 to pass through, and the outer wall of the screw 13 is threadedly connected with a nut 15.

[0030] That is, when installing the filter plate 7, align the plug-in slot seat 12 on the filter plate 7 with the plug-in block 11 on the vertical rod 26, and then insert the plug-in slot seat 12 on the plug-in block 11, so as to limit the filter plate 7. At the same time, the screw 13 on the plug-in block 11 passes through the bottom through hole 14 of the plug-in slot seat 12, and then the nut 15 is rotated clockwise to install the nut 15 on the screw rod 13. The nut 15 moves upward along the screw rod 13 and slowly contacts the bottom end of the plug-in slot seat 12 to complete the installation of the filter plate 7. Conversely, the nut 15 is rotated counterclockwise to remove the nut 15 from the screw rod 13, and then the plug-in slot seat 12 is pulled out from the plug-in block 11, so that the filter plate 7 can be disassembled. The disassembly and assembly operation is relatively convenient, and it is more convenient for the staff to clean and replace the filter plate 7.

[0031] Example 4 In Example 1, the contact effect between the single endotoxin adsorption column 25 and the collagen microsphere solution is poor, resulting in poor endotoxin removal effect. Figure 1 , Figure 2 as well as Figure 7As shown, the present invention designs the endotoxin adsorption column 25 into a cylindrical groove shape, and a plurality of infusion pumps 19 are distributed circumferentially on the inner wall of the treatment tank 3. The infusion pump 19 is provided with a circulation pipe 20 for inputting the solution at the bottom of the treatment tank 3 to the top of the endotoxin adsorption column 25.

[0032] When in use, the infusion pump 19 acts on the circulation tube 20 to input the solution at the bottom of the treatment tank 3 to the top of the endotoxin adsorption column 25, thereby entering its cylindrical groove, and then passing through the edge of the endotoxin adsorption column 25 until it is transported by the circulation tank 20 again. Such repeated circulation can promote the flow of the solution in the treatment tank 3, and can effectively improve the contact effect between the solution and the endotoxin adsorption column 25, thereby improving the removal effect of endotoxin.

[0033] At the same time, a top cover B16 is installed on the top of the processing tank 3, and a stirring paddle B18 driven by a motor B17 is installed on the bottom of the top cover B16. The stirring paddle B18 is placed in the endotoxin adsorption column 25, and the rotation of the stirring paddle B18 can be driven by the motor B17. The stirring paddle B18 is used to promote the flow of the solution in the endotoxin adsorption column 25 again, and the contact effect between the solution and the endotoxin adsorption column 25 is further improved. Moreover, the stirring paddle B18 is set on the top cover B16, and the staff can remove the stirring paddle B18 from the processing tank 3 for cleaning by disassembling the top cover B16.

[0034] The present invention also provides a method for using the above-mentioned endotoxin removal device: Step 1: Connect the collagen microsphere solution infusion pipeline to the liquid inlet pipe 5 on the filter tank 2, so as to input the solution into the filter tank 2; Step 2: Switch the position of the valve core of the electric three-way ball valve A402 through the controller 22, connect the connecting pipe B404 to the two-way water pump 401, and switch the position of the valve core of the electric three-way ball valve B405 at the same time, connect the connecting pipe C406 to the electric two-way water pump 401, input the solution from the filter tank 2 to the treatment tank 3 through the electric two-way water pump 401, and the endotoxin adsorption column 25 in the treatment tank 3 adsorbs the endotoxin in the solution; Step 3: After the adsorption is completed, the position of the valve core of the electric three-way ball valve A402 is switched through the controller 22, and the connecting pipe A403 is connected to the two-way water pump 401. At the same time, the position of the valve core of the electric three-way ball valve B405 is switched, and the connecting pipe D407 is connected to the electric two-way water pump 401. Then, the filtered collagen microsphere solution is input from the bottom of the treatment tank 3 to the top of the filter tank 2 through the electric two-way water pump 401 to be filtered again through the filter plate 7. Step 4: Open the electric valve on the drain pipe 6, and the filtered solution is discharged from the filter tank 2 through the drain pipe 6.

[0035] The endotoxin removal device based on collagen microsphere production and the use method thereof of the present invention have the following advantages: The collagen microsphere solution is filtered by the filter tank 2 to remove large particles of impurities. After the filtration, the solution is input into the treatment tank 3 through the reciprocating pipe assembly 4. The endotoxin adsorption column 25 in the treatment tank 3 adsorbs the endotoxin in the solution. Then, the solution is input into the filter tank 2 by the reciprocating pipe assembly 4 to filter the impurities generated during the treatment process. After the filtration is completed, the solution is discharged from the drain pipe 6. In this way, only one filter tank 2 is needed to complete two filtrations in the whole process, which saves costs and reduces the size of the device, and is very beneficial for use in small factories. The stirring paddle A10 is driven by the motor A9 to rotate, and the stirring paddle A10 stirs the solution above the filter plate 7 by rotating to avoid accumulation of impurities, which can effectively avoid clogging of the filter plate 7; The quick-release structure makes it easier to disassemble and assemble the filter plate 7, making it more convenient for the staff to clean and replace the filter plate 7. The infusion pump 19 acts on the circulation pipe 20 to input the solution at the bottom of the treatment tank 3 to the top of the endotoxin adsorption column 25, thereby entering the cylindrical groove thereof, and then passing through the edge of the endotoxin adsorption column 25 until it is transported by the circulation tank again. This repeated cycle can promote the flow of the solution in the treatment tank 3, and can effectively improve the contact effect between the solution and the endotoxin adsorption column 25, thereby improving the removal effect of endotoxins. The infusion pump 19 acts on the circulation pipe 20 to input the solution at the bottom of the treatment tank 3 to the top of the endotoxin adsorption column 25, thereby entering the cylindrical groove thereof, and then passing through the edge of the endotoxin adsorption column 25 until it is transported by the circulation tank again. This repeated cycle can promote the flow of the solution in the treatment tank 3, and can effectively improve the contact effect between the solution and the endotoxin adsorption column 25, thereby improving the removal effect of endotoxins. The forklift slot 23 on the base 1 can cooperate with a forklift to move the device, and the universal wheels 24 make it easy for workers to push the device, thereby facilitating the movement of the device.

[0036] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any form. Any ordinary technician in the industry can smoothly implement the present invention as shown in the drawings and described above. However, any equivalent changes, modifications and evolutions made by technicians familiar with the profession without departing from the scope of the technical solution of the present invention using the technical content disclosed above are all equivalent embodiments of the present invention. At the same time, any equivalent changes, modifications and evolutions made to the above embodiments based on the essential technology of the present invention are still within the protection scope of the technical solution of the present invention.

Claims

1. An endotoxin removal device based on collagen microsphere production, characterized in that: The invention comprises a base (1) and a filtering tank (2) and a processing tank (3) located on the base (1); a reciprocating pipe assembly (4) is installed between the filtering tank (2) and the processing tank (3), and is capable of reciprocatingly transporting the collagen microsphere solution between the two tanks, so that the collagen microsphere solution entering and exiting the processing tank (3) can be filtered by the filtering tank (2); A liquid inlet pipe (5) is installed at an upper portion of the outer wall of the filter tank (2), a liquid discharge pipe (6) is installed at a lower portion of the outer wall, and a filter plate (7) is installed in the middle of the filter tank (2); An endotoxin adsorption column (25) is installed inside the filter tank (2).

2. The endotoxin removal device based on collagen microsphere production according to claim 1, characterized in that: The reciprocating pipe assembly (4) comprises a two-way water pump (401), one end of the two-way water pump (401) being connected to a connecting pipe A (403) and a connecting pipe B (404) via an electric three-way ball valve A (402), the connecting pipe A (403) being in communication with an upper portion of the interior of the filter tank (2), and the connecting pipe B (404) being in communication with a lower portion of the interior of the filter tank (2); The other end of the two-way water pump (401) is connected to a connecting pipe C (406) and a connecting pipe D (407) via an electric three-way ball valve B (405); the connecting pipe C (406) is in communication with an upper portion of the interior of the treatment tank (3); and the connecting pipe D (407) is in communication with a lower portion of the interior of the treatment tank (3).

3. The endotoxin removal device based on collagen microsphere production according to claim 2, characterized in that: Electric valves are installed on the connecting pipe A (403), the connecting pipe B (404), the connecting pipe C (406), the connecting pipe D (407), the liquid inlet pipe (5) and the liquid discharge pipe (6).

4. The endotoxin removal device based on collagen microsphere production according to claim 1, characterized in that: A top cover A (8) is installed at the top of the filter tank (2); the bottom of the top cover A (8) is connected to the filter plate (7) via vertical rods (26) at all sides; a stirring paddle A (10) driven by a motor A (9) is installed at the bottom of the top cover A (8); the stirring paddle A (10) is located directly above the filter plate (7).

5. The endotoxin removal device based on collagen microsphere production according to claim 4, characterized in that: The bottom end of the vertical rod (26) is fixedly connected to a plug-in block (11); a plurality of plug-in slots (12) matched with the plug-in block (11) are embedded and fixed inside the filter plate (7); the bottom end of the plug-in block (11) is fixedly connected to a screw rod (13); a through hole (14) for the screw rod (13) to pass through is formed at the bottom of the plug-in slot (12); and a nut (15) is threadedly connected to the outer wall of the screw rod (13).

6. The endotoxin removal device based on collagen microsphere production according to claim 1, characterized in that: The endotoxin adsorption column (25) is in the shape of a cylindrical groove. A top cover B (16) is installed at the top of the treatment tank (3), and a stirring paddle B (18) driven by a motor B (17) is installed at the bottom of the top cover B (16). The stirring paddle B (18) is placed in the endotoxin adsorption column (25).

7. The endotoxin removal device based on collagen microsphere production according to claim 6, characterized in that: A plurality of infusion pumps (19) are circumferentially distributed on the inner wall of the treatment tank (3), and the infusion pump (19) is provided with a connecting tube (20) for inputting the solution at the bottom of the treatment tank (3) to the top of the endotoxin adsorption column (25).

8. The endotoxin removal device based on collagen microsphere production according to claim 1, characterized in that: The top end of the base (1) is connected to the controller (22) via a bracket (21).

9. The endotoxin removal device based on collagen microsphere production according to claim 1, characterized in that: Forklift slots (23) are provided on both sides of the front side ends of the base (1), and universal wheels (24) are installed around the bottom of the base (1) to facilitate its movement.

10. A method for using the endotoxin removal device according to any one of claims 1 to 9, characterized in that: The steps include: Step 1: Connecting the collagen microsphere solution infusion pipeline to the liquid inlet pipe (5) on the filter tank (2), thereby inputting the solution into the filter tank (2); Step 2: Switch the position of the valve core of the electric three-way ball valve A (402) through the controller (22), connect the connecting pipe B (404) to the two-way water pump (401), and switch the position of the valve core of the electric three-way ball valve B (405) at the same time, connect the connecting pipe C (406) to the electric two-way water pump (401), and input the solution from the filter tank (2) to the treatment tank (3) through the electric two-way water pump (401), and the endotoxin adsorption column (25) in the treatment tank (3) adsorbs the endotoxin in the solution; Step 3: After the adsorption is completed, the position of the valve core of the electric three-way ball valve A (402) is switched through the controller (22), and the connecting pipe A (403) is connected to the two-way water pump (401). At the same time, the position of the valve core of the electric three-way ball valve B (405) is switched, and the connecting pipe D (407) is connected to the electric two-way water pump (401). Then, the filtered collagen microsphere solution is input from the bottom of the treatment tank (3) to the top of the filtration tank (2) through the electric two-way water pump (401) to be filtered again through the filter plate (7). Step 4: Open the electric valve on the drain pipe (6), and the filtered solution is discharged from the filter tank (2) through the drain pipe (6).

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