A comprehensive measurement system for two-photon fluorescent materials

Abstract

The application discloses a comprehensive measuring system for two-photon fluorescent materials, which comprises a laser, a tunable attenuator, a beam splitter, a reference light integrating sphere, a reference light spectrometer, a focusing mirror, a test light integrating sphere, a test light integrating sphere, a transmission light integrating sphere, a transmission light spectrometer, a stepping motor controller and a computer, etc. The comprehensive measuring system can comprehensively measure various key performance parameters of two-photon fluorescent materials, and can improve the accuracy and reliability of the measurement, thereby providing strong technical support for the research and application of two-photon fluorescent materials.

Description

Technical Field

[0001] This invention relates to the field of parameter measurement of two-photon fluorescent materials, and in particular to a comprehensive measurement system for two-photon fluorescent materials, which can comprehensively measure the two-photon fluorescence spectrum, nonlinear luminescence quantum efficiency, and nonlinear transmittance of two-photon fluorescent materials. Background Technology

[0002] Two-photon fluorescent materials, due to their unique luminescence properties, have shown broad application prospects in numerous fields such as biomedical imaging, optoelectronic devices, and information storage. When evaluating the performance of two-photon fluorescent materials, two-photon fluorescence spectroscopy, quantum efficiency, and nonlinear transmittance are three crucial core parameters. These parameters not only reflect the optical properties of the material but also provide key information for its design and optimization.

[0003] To date, the academic community has made some progress in the performance evaluation methods of two-photon fluorescent materials. For example, the literature [Wang JZ, et al. CdSe / AsS Core-Shell Quantum Dots: Preparation and Two-Photon Fluorescence. J.Am.Chem.Soc.131(32):11300,2009] proposes an effective measurement method for two-photon fluorescence spectra. This method uses a laser to generate a high-intensity laser beam and focuses it onto the sample under test through an optical system. Under the action of two-photon absorption, the sample can effectively absorb light energy and release fluorescence signals. These fluorescence signals are collected by an objective lens system and finally captured, recorded and analyzed by a spectrometer, thereby realizing the accurate measurement of two-photon fluorescence spectra. Although this method has achieved certain results in measuring two-photon fluorescence spectra, its limitations are also obvious: that is, it cannot achieve the simultaneous measurement of quantum efficiency and nonlinear transmittance of two-photon fluorescent materials, which undoubtedly limits the application scope and depth of this method in comprehensively evaluating the performance of two-photon fluorescent materials.

[0004] Therefore, developing a novel method capable of simultaneously measuring these three core parameters is of great significance for promoting the research and application of two-photon fluorescent materials. Summary of the Invention

[0005] To overcome the limitations of existing technologies in measuring the performance of two-photon fluorescent materials, particularly the inability to accurately measure key parameters such as quantum efficiency and nonlinear luminescence power, this invention proposes a comprehensive measurement system for two-photon fluorescent materials. This system can comprehensively measure the two-photon fluorescence spectrum, nonlinear luminescence quantum efficiency, and nonlinear transmittance of two-photon fluorescent materials.

[0006] The technical solution of the present invention is as follows:

[0007] A comprehensive measurement system for two-photon fluorescent materials, characterized by comprising:

[0008] A laser is used to generate a laser beam.

[0009] A tunable attenuator is positioned in the path of the laser beam generated by the laser to adjust the intensity of the laser beam.

[0010] A beam splitter is positioned on the path of the laser beam after it has been adjusted by the tunable attenuator, and is used to split the laser beam into a reference beam and a test beam.

[0011] A reference light integrating sphere is used to collect the reference light;

[0012] A reference light spectrometer, connected to the reference light integrating sphere via an optical fiber, is used to measure the spectral distribution of the reference light;

[0013] A focusing lens is positioned in the path of the test light to focus the test light to a preset focal point;

[0014] A test integrating sphere is placed at the focal point of the focusing lens, and a two-photon fluorescent material sample to be tested is placed inside it to collect the fluorescence emitted by the sample.

[0015] A test optical spectrometer, connected to the test optical integrating sphere via an optical fiber, is used to measure the spectral distribution of the fluorescence;

[0016] A transmission light integrating sphere is disposed behind the test light integrating sphere to collect the test light transmitted from the sample;

[0017] A transmission light spectrometer, connected to the transmission light integrating sphere via an optical fiber, is used to measure the spectral distribution of the transmitted light;

[0018] Stepper motor controllers are used to control the movement and position adjustment of various components in the system;

[0019] A computer, connected to the reference optical spectrometer, the test optical spectrometer, and the transmission optical spectrometer, is used to receive and process spectral data to calculate two-photon fluorescence spectra, nonlinear luminescence quantum efficiency, and nonlinear transmittance.

[0020] Preferably, the tunable attenuator can continuously adjust the intensity of the laser beam to adapt to different measurement requirements.

[0021] Preferably, the beam splitter is capable of splitting the laser beam into two equal beams to ensure that the reference beam and the test beam have the same initial intensity.

[0022] Preferably, the combination of the test light integrating sphere and the test light spectrometer can measure the fluorescence spectrum emitted by the sample under the two-photon absorption effect, and then calculate the quantum efficiency.

[0023] Preferably, the combination of the tunable attenuator, the reference light integrating sphere, the reference light spectrometer, the transmission light integrating sphere, and the transmission light spectrometer can measure the nonlinear transmittance of the sample, specifically by comparing the spectral distributions of the reference light and the transmitted light.

[0024] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0025] (1) This invention achieves comprehensive and accurate measurement of the properties of two-photon fluorescent materials by integrating multiple high-precision components, such as tunable attenuators, beam splitters, integrating spheres, and spectrometers. This includes key parameters such as two-photon fluorescence spectroscopy, nonlinear luminescence quantum efficiency, and nonlinear transmittance.

[0026] (2) The combination of high-precision spectrometer and integrating sphere ensures comprehensive and accurate collection of fluorescence and transmitted light signals, avoiding inaccurate measurement due to incomplete signal collection or excessive error.

[0027] (3) It can measure two-photon fluorescence spectra and absolute luminescence power under different power conditions. Attached Figure Description

[0028] Figure 1 This is a schematic diagram of a traditional system for measuring the two-photon fluorescence spectrum of two-photon fluorescent materials.

[0029] Figure 2 This is a schematic diagram of the integrated measurement system for two-photon fluorescent materials according to an embodiment of the present invention. Detailed Implementation

[0030] The present invention will now be described in detail with reference to the accompanying drawings and specific embodiments, but this should not be construed as limiting the scope of protection of the present invention.

[0031] A comprehensive measurement system for two-photon fluorescent materials includes a laser, a tunable attenuator, a beam splitter, a reference integrating sphere, a reference spectrometer, a focusing lens, a test integrating sphere, a transmission integrating sphere, a transmission spectrometer, a stepper motor controller, and a computer. The tunable attenuator and beam splitter are placed sequentially along the laser beam emission direction. The beam splitter divides the incident beam into a reflected beam and a transmitted beam. The reflected beam serves as the reference beam, and the reference integrating sphere is positioned along its propagation direction. The reference spectrometer is connected to the port of the reference integrating sphere via an optical fiber. The transmitted beam serves as the test beam, and the focusing lens, the test integrating sphere, and the transmission integrating sphere are positioned sequentially along its propagation direction. The test spectrometer is connected to the port of the test integrating sphere via an optical fiber. The transmission spectrometer is connected to the port of the transmission integrating sphere via an optical fiber. The stepper motor controller is connected to the tunable attenuator. The stepper motor controller, the reference optical spectrometer, the test optical spectrometer, and the transmission optical spectrometer are connected to the computer.

[0032] Tunable attenuators, test light integrating spheres, and test light spectrometers are used to measure the fluorescence spectrum and quantum efficiency of samples, while reference light integrating spheres, reference light spectrometers, transmission light integrating spheres, and transmission light spectrometers are used to measure the nonlinear transmittance of samples.

[0033] Detailed Explanation of Working Principle:

[0034] Laser source and beam control: The laser beam generated by the laser first passes through a tunable attenuator, which can precisely adjust the laser intensity to meet different measurement requirements. Subsequently, the laser beam is split into two beams by a beam splitter: one beam is used as a reference beam, and the other is used as a test beam.

[0035] Reference light path: The reference light is directly guided into the reference light integrating sphere, which is responsible for collecting and homogenizing the light intensity. The reference light spectrometer is connected to the reference light integrating sphere via optical fiber to measure the spectral distribution of the reference light, providing a benchmark for subsequent calculations.

[0036] Test light path and sample measurement: After being precisely focused by the focusing lens, the test light illuminates the sample located at the center of the test light integrating sphere. The sample emits fluorescence under the two-photon absorption effect. These fluorescence signals are collected by the test light integrating sphere and transmitted through optical fiber to the test light spectrometer for spectral analysis.

[0037] Transmitted light measurement: Simultaneously, the light transmitted from the sample is collected by the transmission light integrating sphere. The transmission light spectrometer is connected to the transmission light integrating sphere via optical fiber to measure the spectral distribution of the transmitted light, which is crucial for calculating nonlinear transmittance.

[0038] System control and data processing: The stepper motor controller is responsible for precisely controlling the movement and position of each component, ensuring the accuracy and repeatability of the measurements. The computer, as the system's control center, is responsible for receiving data from each spectrometer and performing complex calculations and analyses to derive key parameters such as two-photon fluorescence spectra, nonlinear luminescence quantum efficiency, and nonlinear transmittance.

[0039] The comprehensive measurement system of this invention not only enables the comprehensive measurement of various key performance parameters of two-photon fluorescent materials, but also improves the accuracy and reliability of the measurement, providing strong technical support for the research and application of two-photon fluorescent materials.

[0040] Example:

[0041] The laser is a femtosecond laser with a center wavelength of 800 nm. The tunable attenuator has a transmittance range of 3%-100%. The beam splitter is made of fused silica glass with approximately 10% reflectivity on both the front and back surfaces and 90% transmittance. The reference integrating sphere has an internal diameter of 150 mm, an internal coating with 97% reflectivity, and three openings. The reference spectrometer has a spectral detection range of 350-950 nm and a slit size of 70 μm. The focusing lens has a focal length of 150 mm. The test integrating sphere has an internal diameter of 50 mm, an internal coating with 97% reflectivity, and four openings. The test spectrometer has a spectral detection range of 340-1015 nm. The transmission integrating sphere has an internal diameter of 150 mm, an internal coating with 97% reflectivity, and four openings. The transmission spectrometer has a spectral detection range of 350-950 nm and a slit size of 70 μm.

[0042] The comprehensive measurement system of this invention not only enables the comprehensive measurement of various key performance parameters of two-photon fluorescent materials, but also improves the accuracy and reliability of the measurement, providing strong technical support for the research and application of two-photon fluorescent materials.

Claims

1. A comprehensive measurement system of two-photon fluorescent material, characterized in that, The application relates to a two-photon fluorescence spectrum measurement system. The application comprises: a laser for generating a laser beam; a tunable attenuator arranged in the path of the laser beam generated by the laser for adjusting the intensity of the laser beam; a beam splitter arranged in the path of the laser beam adjusted by the tunable attenuator for splitting the laser beam into reference light and test light; a reference light integrating sphere for collecting the reference light; a reference light spectrometer connected with the reference light integrating sphere through an optical fiber for measuring the spectral distribution of the reference light; a focusing mirror arranged in the path of the test light for focusing the test light to a preset focal point; a test light integrating sphere arranged at the focal point of the focusing mirror and internally placed with a sample of a two-photon fluorescence material to be measured for collecting fluorescence emitted by the sample; a test light spectrometer connected with the test light integrating sphere through an optical fiber for measuring the spectral distribution of the fluorescence; a transmitted light integrating sphere arranged behind the test light integrating sphere for collecting test light transmitted from the sample; a transmitted light spectrometer connected with the transmitted light integrating sphere through an optical fiber for measuring the spectral distribution of the transmitted light; a stepper motor controller for controlling the movement and position adjustment of components in the system; 2. The comprehensive measurement system of two-photon fluorescent material according to claim 1, wherein, a computer connected with the reference light spectrometer, the test light spectrometer and the transmitted light spectrometer for receiving spectral data and processing the spectral data to calculate two-photon fluorescence spectrum, nonlinear luminescence quantum efficiency and nonlinear transmittance.

3. The comprehensive measurement system of two-photon fluorescent material according to claim 1, wherein, The tunable attenuator can continuously adjust the intensity of the laser beam to adapt to different measurement requirements.

4. The comprehensive measurement system of two-photon fluorescent material according to claim 1, wherein, The beam splitter can equally divide the laser beam into two beams to ensure that the reference light and the test light have the same initial intensity. The combination of the test light integrating sphere and the test light spectrometer can measure the fluorescence spectrum emitted by the sample under the two-photon absorption effect, and then calculate the quantum efficiency.

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