Method for promoting tillering of zizania latifolia by using sulfopeptide

Abstract

The invention provides a method for promoting tillering of zizania aquatica by using sulfopeptide, and solves the technical problem that the tillering number of zizania aquatica is small in the prior art. The invention provides a method for promoting tillering of zizania aquatica by utilizing sulfopeptide and application of the sulfopeptide. The method comprises the step of smearing zizania aquatica seedlings with the sulfopeptide. The Zizania aquatica seedlings are coated with the sulfokine, division and proliferation of Zizania aquatica cells can be promoted, more branches can be generated in the tillering stage of the Zizania aquatica, and therefore the tillering and yield of plants are increased. Meanwhile, by smearing the sulfokine on the zizania aquatica seedlings, growth of the zizania aquatica can be promoted by influencing development and functions of plant root systems and improving nutrient absorption of plants and transfer efficiency of the plants in the plants, leaves in the early stage can grow rapidly, the leaf area is increased, and therefore photosynthesis is improved, photosynthesis efficiency is improved, and the yield of the zizania aquatica is increased.

Description

Technical Field

[0001] The invention belongs to the technical field of wild rice stem planting, and particularly relates to a method for promoting tillering of wild rice stem by utilizing sulfopeptide. Background Art

[0002] The tillering of wild rice refers to the fact that during the growth of wild rice, its stem will grow new branches from the base or nodes. This phenomenon is called "tillering" in botany, which is a natural way for plants to grow. It helps plants expand their growth area and increase photosynthesis, thereby increasing the growth rate and yield of plants. Wild rice has a strong tillering ability, which is one of its growth characteristics. A small number of tillers will affect its yield and quality. Therefore, the number of tillers is very important for the growth and yield of wild rice.

[0003] At present, the methods to promote tillering of wild rice include irrigation management and fertilization management. For example, shallow water of 3-5 cm is applied in the early stage of tillering, combined with heavy topdressing to promote the growth of effective tillers. Excessive irrigation or improper water management may lead to the occurrence of diseases and pests, affecting the healthy growth of wild rice. Improper fertilization, such as excessive application of nitrogen fertilizer, may cause excessive growth of plants, excessive tillering, affecting the pregnancy of wild rice, and reducing yield and quality. Summary of the invention

[0004] The invention provides a method for promoting tillering of Zizania latifolia by utilizing sulfopeptide, aiming to solve the technical problem of small tillering number of Zizania latifolia in the prior art.

[0005] In order to solve the above technical problems, the technical solution adopted by the present invention is:

[0006] In a first aspect, the present invention provides a method for promoting tillering of Zizania latifolia by using sulfopeptide, comprising: spraying or applying the sulfopeptide on the Zizania latifolia seedlings.

[0007] In a preferred embodiment, the concentration of the sulfopeptide is 0.5-2.0 μmol / L.

[0008] Based on the above scheme, the appropriate concentration can enhance the effect of sulfopeptide and promote the tillering of water bamboo without causing abnormal growth of water bamboo.

[0009] In a preferred embodiment, the concentration of the sulfopeptide is 0.5 μmol / L.

[0010] In a preferred embodiment, the concentration of the sulfopeptide is 1.0 μmol / L.

[0011] In a preferred embodiment, the concentration of the sulfopeptide is 1.5 μmol / L.

[0012] In a preferred embodiment, the concentration of the sulfopeptide is 2.0 μmol / L.

[0013] In a preferred embodiment, the sulfopeptide is sprayed or applied on the leaves of wild rice seedlings for 13-15 days.

[0014] Based on the above scheme, experiments have shown that applying sulfonyl peptide on the leaves of Zizania latifolia seedlings for 14 days has a significant promoting effect, and the average tillering number content increase rate is relatively high.

[0015] In a preferred embodiment, the water chestnut seedlings are water chestnut seedlings at the 4-leaf stage.

[0016] Based on the above scheme, the 4-leaf stage is a critical period for the growth of Zizania latifolia. At this time, the plant begins to enter a rapid growth stage and is more sensitive to external stimuli. Applying sulfonyl peptide at this time can promote its tillering.

[0017] In a preferred embodiment, the 4-leaf stage Zizania latifolia seedlings are obtained under the following culture conditions: culture temperature of 22-25° C., photoperiod of 12 h, and culture time of 20-25 days.

[0018] Based on the above scheme, for the rice seedlings, 22-25℃ can ensure the normal metabolism and growth activities of the rice seedlings. The 12-hour light cycle provides sufficient light time for the rice seedlings, which is conducive to photosynthesis and thus promotes growth. At the same time, this light cycle helps to induce the tillering of the rice seedlings.

[0019] In a second aspect, the present invention provides the use of sulfopeptide in promoting tillering of Zizania latifolia.

[0020] The beneficial effects of the present invention are:

[0021] The present invention provides a method for promoting tillering of wild rice stem by using sulfonate, and by spraying or applying sulfonate on wild rice stem seedlings, the division and proliferation of wild rice stem cells can be promoted, and the wild rice stem can be helped to produce more branches during the tillering period, thereby increasing the yield of the plant. At the same time, applying sulfonate on the wild rice stem seedlings can promote the growth of wild rice stem by affecting the development and function of the plant root system, improving the plant's absorption of nutrients and the transport efficiency in the plant body, making the early leaves grow rapidly, increasing the leaf area, thereby increasing photosynthesis, improving the efficiency of photosynthesis, and thus increasing the yield of wild rice stem. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for use in the embodiments are briefly introduced below. It should be understood that the following drawings only show certain embodiments of the present invention and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other relevant drawings can be obtained based on these drawings without paying creative work.

[0023] Figure 1 This is a schematic diagram of the present invention for investigating the effect of sulfonyltransferase on the tillering number of Zizania latifolia. DETAILED DESCRIPTION

[0024] The following will be combined with the accompanying drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative work belong to the protection scope of the present invention.

[0025] Embodiment 1:

[0026] This embodiment provides a method for promoting tillering of Zizania latifolia by using sulfopeptide, comprising: spraying or applying the sulfopeptide on the Zizania latifolia seedlings. Applying the sulfopeptide on the Zizania latifolia seedlings.

[0027] Wherein, the concentration of the sulfopeptide is 0.5-2.0 μmol / L.

[0028] Specifically, the concentration of the sulfopeptide can be 0.5 μmol / L, 1.0 μmol / L, 1.5 μmol / L or 2.0 μmol / L. A preferred solution is that the concentration of the sulfopeptide is 1.0 μmol / L.

[0029] The sulfonyl peptide is sprayed or applied on the leaves of the wild rice seedlings for 13-15 days.

[0030] Specifically, the sulfonyl peptide can be applied on the leaves of the wild rice seedlings for 13 days, 14 days or 15 days. A more optimal solution is that the sulfonyl peptide is applied on the leaves of the wild rice seedlings for 14 days.

[0031] In addition, the rice stem seedlings are cultured for 20-25 days under the conditions of a temperature of 22-25° C. and a photoperiod of 12 hours to obtain a 4-leaf stage, and sulfopeptide is applied to the leaves of the rice stem seedlings at the 4-leaf stage.

[0032] Embodiment 2: The present invention provides the use of sulfopeptide in promoting tillering of Zizania latifolia.

[0033] The present invention will be further explained below in conjunction with specific experiments:

[0034] 1. Material preparation

[0035] Jinjiao No. 10 Zizania latifolia seedlings, sulfaquinone (PSK), sterile water

[0036] 2. Test process

[0037] S1. Select healthy growing Jinjiao No. 10 wild rice seedlings and culture them at a temperature of 24°C and a photoperiod of 12 hours of light / 12 hours of darkness. After 3 weeks of culture, take seedlings with consistent growth, apply 1.0 μmol / L PSK to the plants in the treatment group (PSK), and treat the leaves of the control group (CK) with sterile water. Conduct relevant tests one week later.

[0038] S2. Plant the wild rice seedlings in the field, continuously observe the number of tillers, and count the number of tillers every week.

[0039] 3. Test results

[0040] As shown in Table 1 and Figure 1 As shown in the figure, at each treatment stage, the number of tillers in the PSK group was higher than that in the control group, and the average tiller number in the PSK group at 14d, 28d, and 42d treatments increased by 122.22%, 29.03%, and 30.91% respectively compared with the control group, with the most significant promotion effect at 14d. In summary, exogenous PSK can have a continuous promoting effect on the tillering number of Zizania latifolia, which is more obvious in the early and middle stages. As the treatment time increases, the advantage of the PSK group decreases, which may be due to the gradual enhancement of the tillering ability of Zizania latifolia in the control group. However, the application of exogenous PSK always maintains a positive effect on the tillering of Zizania latifolia.

[0041] Table 1

[0042]

[0043] 4. Test conclusion

[0044] The exogenous plant hormone PSK has a significant promoting effect on the tillering number of Zizania latifolia, which indicates that PSK can be used as an effective growth regulator to promote the tillering development of Zizania latifolia. But it also has a time duration. The promoting effect of PSK is most significant in the early and middle stages of the treatment, especially on the 14th day after treatment. As time goes by, although the number of tillers in the PSK group is still higher than that in the control group, this advantage has decreased; but although the advantage of the PSK group has decreased over time, the positive effect of PSK on Zizania latifolia tillering is sustained. This shows that even after a longer treatment time, PSK can still maintain a positive effect on Zizania latifolia tillering. As an exogenous plant hormone, PSK has potential application value in Zizania latifolia cultivation, especially in promoting crop tillering and increasing yield.

[0045] The present invention is not limited to the above-mentioned optional implementation modes. Anyone can derive other various forms of products under the inspiration of the present invention. However, no matter what changes are made in the shape or structure, all technical solutions that fall within the scope defined by the claims of the present invention fall within the protection scope of the present invention.

Claims

1. A method for promoting tillering of wild rice stem using sulfopeptide, characterized in that: include: Spray or apply sulfopeptide on the water chestnut seedlings. Apply sulfopeptide on the water chestnut seedlings.

2. A method for promoting tillering of wild rice stem using sulfopeptide according to claim 1, characterized in that: The concentration of the sulfopeptide is 0.5-2.0 μmol / L.

3. A method for promoting tillering of wild rice stem using sulfopeptide according to claim 2, characterized in that: The concentration of the sulfopeptide is 0.5 μmol / L.

4. A method for promoting tillering of wild rice stem using sulfopeptide according to claim 2, characterized in that: The concentration of the sulfopeptide is 1.0 μmol / L.

5. A method for promoting tillering of wild rice stem using sulfopeptide according to claim 2, characterized in that: The concentration of the sulfopeptide is 1.5 μmol / L.

6. A method for promoting tillering of wild rice stem using sulfopeptide according to claim 2, characterized in that: The concentration of the sulfopeptide is 2.0 μmol / L.

7. A method for promoting tillering of wild rice stem using sulfopeptide according to claim 1, characterized in that: The sulfonyl peptide is sprayed or applied on the leaves of the wild rice seedlings for 13-15 days.

8. The method for promoting tillering of wild rice stem using sulfopeptide according to claim 1, characterized in that: The water chestnut seedlings are water chestnut seedlings in the 4-leaf stage.

9. The method for promoting tillering of wild rice stem using sulfopeptide according to claim 5, characterized in that: The 4-leaf stage Zizania latifolia seedlings are obtained under the following culture conditions: culture temperature of 22-25° C., photoperiod of 12 h, and culture time of 20-25 days.

10. Application of sulfopeptide in promoting tillering of Zizania latifolia.

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