Exogenous hormone formula for promoting cutting rooting of pinus massoniana

Abstract

The invention discloses an exogenous hormone formula for promoting cutting rooting of pinus massoniana, and relates to the technical field of plant cutting, and the technical scheme is as follows: the exogenous hormone formula comprises the following components in percentage by mass: 40% of IBA, 40% of NAA and 20% of D-iso-VC; the preparation method comprises the following steps: weighing 0.4 g of IBA, 0.4 g of NAA and 0.2 g of D-iso-VC, dissolving the IBA, the NAA and the D-iso-VC in 50ml of pure ethanol, and adding distilled water to a constant volume of 500ml after 20 minutes of thorough dissolution so as to prepare mother liquor; the mother liquor needs to be diluted when being used, and a prepared solution is obtained by diluting the mother liquor and distilled water according to the proportion of 1: 4; the using process of the prepared solution comprises the steps that after a masson pine semi-lignified scion is subjected to elution treatment, the lower end of the masson pine semi-lignified scion is soaked in the prepared solution for 15 min, and then cuttage is conducted. The method can efficiently solve the problem of low cutting rooting rate of the pinus massoniana, promotes vegetative propagation of the pinus massoniana, and effectively keeps and utilizes excellent characters of parents.

Description

Technical Field

[0001] The invention relates to the technical field of plant cuttings, and more particularly to an exogenous hormone formula for promoting rooting of Masson pine cuttings. Background Art

[0002] Masson pine (Pinus massoniana Lamb.), also known as fir tree, green pine, thick-barked pine, etc., has become the main afforestation tree species in 14 southern provinces because of its strong adaptability, fast growth, high yield, high comprehensive utilization of the whole tree, and excellent fiber. It occupies a very important position in providing rosin and papermaking raw materials and building materials.

[0003] At present, Masson pine mainly relies on seeds for reproduction and is a tree species that is difficult to reproduce asexually. However, in production, sexual reproduction easily leads to trait separation and cannot maintain the excellent traits of the parents. Asexual reproduction can solve this problem, while cutting propagation commonly used in asexual reproduction is affected by various factors, and it is difficult for Masson pine cuttings to take root. Therefore, improving the reproduction coefficient of superior tree materials has become a major problem that must be solved for Masson pine to utilize existing excellent genetic resources in production and scientific research.

[0004] The rooting of cuttings is affected by various factors, such as the physical and chemical properties of the spikelets, exogenous hormones, cutting medium, water content after cutting, temperature, humidity, etc. Among them, the type, concentration and ratio of exogenous hormones used to treat the spikelets during cuttings play an important role.

[0005] In the existing document "Research on the cutting propagation technology of high-yield Masson pine" (He Boxiang et al., Guangdong Forestry Science and Technology, 2004, 20 (1): 16-18), the cutting cut was dipped in self-prepared rooting powder (indolebutyric acid, naphthaleneacetic acid, talcum powder and activated carbon in a certain proportion), and the rooting rate of two-year-old semi-lignified branches reached 75.8%; in "The effect of cutting diameter and elution treatment on the rooting of Masson pine cuttings" (Li Yongjin et al., Journal of Central South University of Forestry and Technology, 2008, 2 (1): 104-107), the rooting rate of two-year-old semi-lignified cuttings treated with ABT1 rooting powder can reach up to 89.5%. The rooting rate of two-year-old semi-lignified cuttings treated with the growth regulator of this formula is significantly improved compared with the rooting rate of two-year-old semi-lignified cuttings in the above documents. How to improve the rooting rate of cuttings is of great significance to the asexual reproduction of Masson pine and maintaining the excellent traits of the parent; therefore, the present invention aims to provide an exogenous hormone formula that promotes the rooting of Masson pine cuttings. Summary of the invention

[0006] The invention aims to provide an exogenous hormone formula for promoting rooting of Masson pine cuttings, aiming to solve the problem of low rooting rate of Masson pine cuttings, so as to promote asexual reproduction of Masson pine and effectively maintain and utilize the excellent traits of the parent.

[0007] The above technical purpose of the present invention is achieved through the following technical scheme: an exogenous hormone for promoting rooting of Masson pine cuttings, comprising the following components, calculated by mass percentage: 40% IBA, 40% NAA and 20% D-isoVC.

[0008] The present invention also provides a method for preparing an exogenous hormone for promoting rooting of Masson pine cuttings. The preparation method comprises the following steps: weighing 0.4g IBA, 0.4g NAA and 0.2g D-isoVC, dissolving them in 50ml pure ethanol, and adding distilled water to make the volume to 500ml after completely dissolving them for 20 minutes to prepare a mother solution.

[0009] The present invention is further configured as follows: the mother liquor needs to be diluted when used, and the prepared solution is obtained by diluting the mother liquor to distilled water at a ratio of 1:4.

[0010] The present invention is further configured as follows: the use process of the prepared solution is: after the semi-lignified spikelets of Masson pine are washed, the lower end is soaked in the prepared solution for 15 minutes and then cut.

[0011] The invention also provides a method for preparing an exogenous hormone for promoting rooting of Masson pine cuttings and an application of the method in promoting rooting of Masson pine cuttings.

[0012] In summary, the present invention has the following beneficial effects:

[0013] 1. The exogenous hormone solution prepared by the present invention can make the rooting rate of Masson pine cuttings reach 96.6% after treating the cuttings, which is 7.9%-27.4% higher than the rooting rate of the same two-year-old semi-lignified cuttings in the existing literature;

[0014] 2. The medicine used in the present invention is easy to purchase, easy to prepare, low-cost, time-saving and labor-saving. After being treated by the present invention, the rooting rate of Masson pine cuttings can be improved. DETAILED DESCRIPTION

[0015] The present invention is described in further detail below.

[0016] Example 1: An exogenous hormone for promoting rooting of Masson pine cuttings, comprising the following components, calculated by mass percentage: 40% IBA, 40% NAA and 20% D-isoVC.

[0017] Example 2: A method for preparing an exogenous hormone for promoting rooting of Masson pine cuttings, comprising weighing 0.4g IBA, 0.4g NAA, and 0.2g D-isoVC, dissolving them in 50ml pure ethanol, and adding distilled water to make up to 500ml after complete dissolution for 20 minutes to obtain a mother liquor that can soak approximately 8,000 cuttings.

[0018] When using, dilute the solution and distilled water in a ratio of 1:4.

[0019] In this embodiment, the method for using the prepared solution is to wash the semi-lignified cuttings of Masson pine and then soak the lower end in the solution for 15 minutes before cutting.

[0020] In order to further illustrate the beneficial effects of the present invention, this embodiment carried out several types of solution ratio treatment tests, as shown in Table 1:

[0021] Table 1 Formula and ratio of each solution

[0022] serial number Ingredient 1 Ingredient 2 Ingredient 3 1 IBA (40%) NAA (40%) D-isovitamin C 2 IBA / / 3 IBA NAA /

[0023] Among them, No. 1 is this formula, No. 2 is IBA solution, and No. 3 is ABT1 rooting powder. After the cuttings were treated with the formula solution, they were cultured under the same conditions. The rooting effects of the cuttings after treatment with each solution are shown in Table 2:

[0024] Table 2 Rooting effect of cuttings after treatment with different solutions

[0025] serial number Number of cuttings Number of rooted plants Rooting rate Number of roots Longest root length (cm) 1 29 28 0.965517 25 21.8 2 30 13 0.433333 / / 3 21 17 0.809524 1 0.5

[0026] After the Masson pine cuttings are soaked in the solution of the invention, the rooting rate can reach 96.5%, which is 122.9% higher than that of the treatment with the IBA solution of the same concentration and 19.1% higher than that of the treatment with the ABT1 solution of the same concentration.

[0027] Conclusion: After the cuttings are treated with the solution prepared by this formula, the rooting rate of Masson pine cuttings can reach 96.6%, and the drugs used in this formula are easy to purchase, easy to prepare, low-cost, time-saving and labor-saving, and can significantly improve the rooting rate of plant cuttings.

[0028] This specific embodiment is merely an explanation of the present invention and is not a limitation of the present invention. After reading this specification, those skilled in the art may make non-creative modifications to the present embodiment as needed. However, as long as they are within the scope of the claims of the present invention, they are protected by the patent law.

Claims

1. An exogenous hormone for promoting rooting of Masson pine cuttings, characterized in that: The following components are included, calculated by mass percentage: 40% IBA, 40% NAA and 20% D-isoVC.

2. A method for preparing an exogenous hormone that promotes rooting of Masson pine cuttings according to claim 1, characterized in that: The preparation method comprises the following steps: weighing 0.4 g IBA, 0.4 g NAA and 0.2 g D-isoVC, dissolving them in 50 ml pure ethanol, and adding distilled water to make the volume to 500 ml after completely dissolving them for 20 minutes to obtain a mother solution.

3. A method for preparing an exogenous hormone that promotes rooting of Masson pine cuttings according to claim 2, characterized in that: The mother solution needs to be diluted when used, and the prepared solution is obtained by diluting the mother solution to distilled water at a ratio of 1:

4.

4. A method for preparing an exogenous hormone that promotes rooting of Masson pine cuttings according to claim 3, characterized in that: The use process of the prepared solution is: after washing the semi-lignified spikelets of Masson pine, the lower end is soaked in the prepared solution for 15 minutes before cutting.

5. Application of the preparation method of an exogenous hormone for promoting rooting of Masson pine cuttings according to any one of claims 2 to 4 in promoting rooting of Masson pine cuttings.