Miniature dyeing equipment

Abstract

The invention belongs to the technical field of dyeing instruments, and discloses miniature dyeing equipment which comprises a protective shell, and a control module used for controlling the equipment to operate is arranged in the protective shell. The drawing plate is arranged in the protective shell in a drawable mode, and a slide used for bearing to-be-dyed tissue is arranged in the drawing plate in a positioning mode; the positioning table is rotatably arranged on the protective shell, and a rotating head corresponding to the positioning table up and down is rotatably arranged on the protective shell in a lifting manner; the consumable box is positioned and mounted on the positioning table, the rotating head is in transmission connection with the consumable box, and a plurality of containers for containing dyeing consumables are arranged in the consumable box; the consumable pressing head corresponds to the tissue on the slide up and down and is used for pressing the container to quantitatively supply dyeing consumables to the tissue. The device is compact in structure, small in equipment size, convenient to carry, suitable for different use environments, capable of accurately controlling the output quantity of reagents, improving the dyeing precision and reducing waste, convenient, rapid, easy to operate and suitable for the fields of laboratories, hospitals, industries and the like.

Description

Technical Field

[0001] The invention belongs to the technical field of dyeing instruments, and in particular relates to a miniature dyeing device. Background Art

[0002] The purpose of staining is to use one or more dyes to stain tissues and intracellular structures differently, so that the internal structure of cells can be clearly observed under a microscope and correct judgment can be made.

[0003] Common staining equipment generally includes fixed stainers and portable stainers; fixed stainers are large-scale staining equipment with high staining efficiency, good staining uniformity, high degree of automation and good safety, but large-scale staining equipment is large in size, high in cost, complicated to operate and inconvenient to use; portable stainers are characterized by portability and flexibility, but the design of portable stainers is based on their portable characteristics, which will lead to uneven tissue staining and reduce staining quality, while increasing staining time, increasing reagent consumption, and reducing the low degree of automation of staining, making it difficult to meet the needs of fast, efficient and precise staining.

[0004] Therefore, the present application designs a miniature dyeing device to solve the above-mentioned technical problems. Summary of the invention

[0005] In order to solve the above technical problems, the present invention proposes a miniature dyeing device.

[0006] To achieve the above object, the present invention provides a miniature dyeing device, comprising:

[0007] A protective housing, wherein a control module for controlling the operation of the device is disposed in the protective housing;

[0008] A pull-out plate, which can be pulled out and arranged in the protective shell, and a glass slide for carrying the tissue to be stained is positioned and arranged in the pull-out plate;

[0009] A positioning platform, the positioning platform is rotatably arranged on the protective shell, and a rotating head is provided on the protective shell to be lifted and rotated corresponding to the positioning platform;

[0010] A consumables box, wherein the consumables box is positioned and mounted on the positioning platform, the rotating head is in transmission connection with the consumables box, and a plurality of containers for containing dyeing consumables are arranged in the consumables box;

[0011] A consumables pressing head, the consumables pressing head corresponds to the tissue on the glass slide up and down, and is used to press the container to quantitatively supply staining consumables to the tissue.

[0012] Preferably, a ventilation module is provided in the protective shell, and the ventilation module is arranged below the pull-out plate, for blowing the consumable liquid on the glass slide off the glass slide, discharging it along the drainage channel arranged in the protective shell, and storing it in a waste water box in the protective shell.

[0013] Preferably, a working platform is provided on the protective shell, a positioning groove is opened on the working platform, the positioning platform is rotatably connected in the positioning groove, the positioning groove is adapted to the consumable box, and during installation, the consumable box slides into the inner wall of the positioning groove and is positioned on the positioning platform.

[0014] Preferably, a guide block is provided in the positioning groove, and a plurality of limit grooves are provided on the guide block, and the limit grooves are provided corresponding to the through grooves provided through the positioning platform, and the outlet of the container extends out of the consumable box and the limit grooves and the through grooves are provided correspondingly.

[0015] Preferably, a plurality of filling slots for filling the container are arranged in the consumable box, and a liquid outlet is arranged at the bottom end of the container, and the liquid outlet extends out of the filling slots.

[0016] Preferably, a clearance groove and a fixing card are provided at the top of the consumable box, the clearance groove is provided on the side wall of the consumable box and corresponds to the rotating head, the fixing card is opened on one side of the clearance groove and is fixed at the top of the consumable box, and when the consumable box is installed, the rotating head passes through the clearance groove and is clamped in the fixing card.

[0017] Preferably, a pressing motor is arranged in the protective shell, the output shaft of the pressing motor is connected to a driving gear, the driving gear is meshed with a longitudinally arranged driven rod, and the consumable pressing head is fixedly installed at the bottom end of the driven rod to drive the consumable pressing head to squeeze the container.

[0018] Preferably, a guide cylinder corresponding to the driven rod is provided in the protective shell, and the driven rod is slidingly connected in the guide cylinder; a longitudinally arranged driven rack is provided on the driven rod, and the driven rack is exposed from the exposed groove of the side wall of the guide cylinder, and the driving gear extends into the exposed groove and meshes with the driven rack for transmission.

[0019] Preferably, a rotating shaft is provided on the protective shell, the top end of the rotating shaft is drivingly connected to a rotating motor fixedly installed in the protective shell, and the bottom end of the rotating shaft is fixedly connected to the consumable pressing head.

[0020] Preferably, a lifting bearing is arranged in the protective shell, the outer ring of the lifting bearing slides longitudinally with the protective shell, and the rotating shaft passes through the lifting bearing and is fixedly connected with the inner ring of the lifting bearing.

[0021] Compared with the prior art, the present invention has the following advantages and technical effects: the present invention discloses a miniature staining device, wherein the protective shell is used for counterweighting, protecting the stability of the device, protecting the internal mechanism, and providing a base for the installation of the internal structure; a glass slide for carrying a tissue sample to be stained is arranged in a pull-out plate, and the pull-out plate is pulled out and arranged in the protective shell, which can be conveniently taken, placed and positioned, and is flexible and convenient to use; the positioning table and the rotating head are correspondingly arranged up and down, and the consumable box is arranged between the positioning table and the rotating head, and can be rotated at a fixed angle under the drive of the two, so that the consumable box positioned and fixed between the two can rotate at a fixed angle accordingly, so that the container containing the staining consumables can be aligned with the sample in turn, and the quantitative supply of the staining consumables is realized under the quantitative squeezing of the consumable pressure head, the output of the staining consumables is accurately controlled, and the accuracy of staining is improved.

[0022] The invention has a compact structure, a small device size, is easy to carry, and is suitable for different use environments. At the same time, it can accurately control the output of the reagent, improve the accuracy of dyeing, reduce waste, is convenient and fast, and is easy to operate. It is suitable for laboratories, hospitals, industries and other fields. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] The drawings constituting a part of the present application are used to provide a further understanding of the present application. The illustrative embodiments and descriptions of the present application are used to explain the present application and do not constitute an improper limitation on the present application. In the drawings:

[0024] Figure 1 This is a schematic diagram of the unfolded state of the drawer plate of the miniature dyeing equipment of the present invention;

[0025] Figure 2 This is a schematic diagram of the closed state of the drawer plate of the miniature dyeing equipment of the present invention;

[0026] Figure 3 This is an axial view of the internal structure of the miniature dyeing device of the present invention;

[0027] Figure 4 It is a schematic cross-sectional view of the structure of the miniature dyeing device of the present invention;

[0028] Figure 5 It is a schematic diagram of the structure of the positioning platform of the present invention;

[0029] Figure 6 This is a schematic diagram of the shielding plate structure of the present invention;

[0030] Figure 7 This is a view of the pressing motor shaft of the present invention;

[0031] Figure 8 This is an axial view of the consumable box of the present invention;

[0032] Fig. 9A top view of the consumable box of the present invention;

[0033] Fig.10 For the present invention Fig. 9 Schematic diagram of the cross section of AA;

[0034] Fig.11 For the present invention Fig. 9 Schematic cross-section of the middle BB;

[0035] In the figure: 1. protective shell; 2. pull-out plate; 3. positioning table; 4. consumable box; 5. consumable pressure head; 6. rotating head; 7. glass slide; 8. container; 9. ventilation module; 10. drainage channel; 11. wastewater box; 12. first handle; 13. second handle; 14. positioning groove; 15. guide block; 16. limit groove; 17. through groove; 18. filling groove; 19. liquid outlet; 20. control ball; 21. give way groove; 22. fixing card; 23. third handle; 24. pressing motor; 25. driving gear; 26. driven rod; 27. guide cylinder; 28. driven rack; 29. ​​rotating shaft; 30. rotating motor; 31. lifting bearing; 32. shielding plate; 33. first button; 34. second button; 35. control module; 36. guide pipe; 37. power supply module. DETAILED DESCRIPTION

[0036] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0037] Dyeing equipment

[0038] In the medical system, staining technology is a vital technology. It is widely used in pathology, histology, cytology and other fields, providing an important basis for the diagnosis and treatment of diseases.

[0039] 1. Purpose and principle of dyeing

[0040] The main purpose of staining is to improve the resolution of each part of the specimen under an optical microscope, making the cell and tissue structure more clearly visible. The principle is to use dyes to chemically react or physically adsorb certain components in cells or tissues, thereby changing the refractive index of these components to light, making them appear in different colors under a microscope.

[0041] 2. Commonly used dyeing methods

[0042] HE staining: Hematoxylin-eosin staining is one of the most commonly used staining methods in histology. Hematoxylin mainly stains the cell nucleus, making it appear blue or purple; eosin stains the cytoplasm and other tissue structures, making them appear pink. HE staining is simple and easy to perform, and can provide information about cell morphology and tissue structure. It is the basis for pathological diagnosis, assessment of tissue damage, and determination of tumor type.

[0043] Special staining: Special staining has richer colors. This staining method, also called histochemical staining, can directly display different special chemical substances inside and outside the cell. For example, reticular fiber staining is used for differential diagnosis of cancer and sarcoma; elastic fiber staining is used to observe whether lung cancer invades the pleura; acid-fast staining is used to find tuberculosis bacteria in tissues; PAS staining is used for diagnosis and differential diagnosis of fungal infections, etc. Special staining can show cell structures or tissue components that cannot be observed by conventional staining, which helps to identify tissue or cell lesions and provide more accurate information for diagnosis and treatment.

[0044] Immunohistochemical staining: Using the specific binding principle between antigens and antibodies, a chemical substance in tissues or cells is extracted as an antigen, and specific antibodies are obtained by immunizing animals. These antibodies are then used to detect similar antigenic substances in tissues or cells. Immunohistochemical staining can provide information about specific proteins, including expression and location, which is of great significance for studying gene expression, cell differentiation, tumorigenesis, etc.

[0045] 3. Dyeing process and precautions

[0046] Process: Staining usually includes dehydration, humidification, slicing, spreading, scooping, staining, dehydration, transparency, sealing and other steps. Among them, the quality of the slices has a great influence on the staining results, so before slicing, you need to ensure that the blade is flat and sharp, and when slicing, pay attention to the direction of the tissue layer and texture parallel to the slicer.

[0047] Note: During the staining process, you need to pay attention to the dye concentration, staining time, temperature and other conditions to ensure the accuracy and stability of the staining results. At the same time, for special samples or experimental requirements, you may need to use special staining methods or adjust the staining conditions.

[0048] 4. Application and development of dyeing

[0049] Application: Staining technology has a wide range of application value in the medical system. It is not only used for the diagnosis and differential diagnosis of diseases, but also for evaluating the degree of tissue damage, determining tumor types, studying cell differentiation, etc. In addition, with the continuous development of medical technology, staining technology is constantly expanding new application areas.

[0050] Development: In recent years, with the rapid development of molecular biology, genetics and other disciplines, staining technology has also been continuously innovated and improved. For example, fluorescence in situ hybridization (FISH), gene chips and other technologies have gradually been applied to clinical pathological diagnosis, providing a more accurate and reliable means for the diagnosis and treatment of diseases.

[0051] Advantages and disadvantages of large-scale dyeing equipment:

[0052] advantage

[0053] High throughput: Large medical staining equipment can usually process multiple samples at the same time, significantly improving staining efficiency; for laboratories that need to process large-scale samples, this can greatly shorten the staining cycle and improve overall work efficiency.

[0054] Good staining uniformity: This type of equipment usually uses advanced staining technology and control systems to ensure that the sample is evenly heated and exposed to the dye during the staining process, thereby achieving uniformity and consistency in the staining results. This helps to improve the accuracy and reliability of diagnosis.

[0055] High degree of automation: Large medical staining equipment is often equipped with an automated control system that can automate the entire process from sample loading, staining, rinsing to drying, etc. This reduces the complexity of manual operation, reduces human errors, and improves the stability and repeatability of staining quality.

[0056] Versatility: Some high-end large-scale staining equipment also has multiple staining modes and functions to meet different sample types and staining needs; this makes the equipment more flexible and adaptable, and can meet the diverse needs of different laboratories.

[0057] High safety: Large medical staining equipment is usually equipped with a complete safety system, such as overheat protection, leakage protection, emergency shutdown and other functions to ensure the safety and stability of the staining process. This helps to protect the safety of operators while preventing equipment damage and sample contamination.

[0058] shortcoming

[0059] High cost: Large medical staining equipment is usually expensive, which may be a burden for laboratories with limited budgets. In addition, the maintenance and repair costs of the equipment may also be high, requiring more funds and resources.

[0060] Complex operation: Although the equipment has a high degree of automation, operation and maintenance still require certain professional knowledge and skills. For operators who lack relevant experience, it may take a long time of training and practice to master the operation and maintenance methods of the equipment.

[0061] Large space occupation: Medical large-scale staining equipment is usually large in size and requires a large laboratory space. This may be a challenge for laboratories with limited space, and it is necessary to rationally plan the laboratory layout and equipment placement.

[0062] High reagent consumption: Although the equipment has efficient staining capabilities, it also consumes a large amount of reagents such as stains and rinse solutions; this increases the cost of the experiment and may cause certain pollution to the environment. Therefore, it is necessary to use reagents reasonably and dispose of waste properly.

[0063] Advantages and disadvantages of portable dyeing equipment;

[0064] A portable medical stainer is a staining device specially designed for use in the medical field. It is characterized by portability and flexibility, and can meet staining needs in different scenarios.

[0065] 1. Main features

[0066] Portability: The portable medical stainer is small in size and light in weight, easy to carry and move, and can meet the staining needs of different places, such as field medical rescue, mobile laboratories, etc.

[0067] Flexibility: The equipment usually has a variety of staining modes and functions, which can be adjusted and set according to different sample types and staining requirements to achieve personalized staining solutions.

[0068] Degree of automation: Although they are portable devices, many portable medical stainers still have a certain degree of automation functions, such as automatic sample loading, automatic staining, automatic rinsing, etc., which reduces the complexity of manual operation.

[0069] Accuracy: The portable medical stainer adopts advanced staining technology and control system, which can ensure the accuracy and consistency of staining results and improve the reliability of diagnosis.

[0070] 2. Application Scenarios

[0071] Field medical rescue: In emergency situations such as natural disasters and wars, portable medical stainers can quickly provide staining services to support the diagnosis and treatment of the wounded and sick.

[0072] Mobile laboratories: In research, teaching, or disease monitoring projects, mobile laboratories may need to change locations frequently. The portability and flexibility of portable medical stainers make them ideal for mobile laboratories.

[0073] Primary medical institutions: In some remote areas or primary medical institutions, due to limited resources, it may not be possible to equip large-scale staining equipment. Portable medical stainers can be used as an alternative to meet the staining needs of these institutions.

[0074] 3. Notes

[0075] Operating specifications: Although the portable medical stainer has automation functions, the operator still needs to operate it according to the instructions and specifications to ensure the accuracy and safety of the staining results.

[0076] Maintenance: Clean, inspect and repair the equipment regularly to ensure normal operation of the equipment and extend its service life.

[0077] Reagent management: Rationally use and manage dyes, flushing solutions and other reagents to avoid waste and pollution.

[0078] 4. Market Trends

[0079] With the continuous advancement of medical technology and people's increasing demand for health, the market demand for portable medical stainers is also increasing. In the future, portable medical stainers will be more intelligent, automated and multifunctional to meet more diverse staining needs. At the same time, with the improvement of environmental awareness, green and environmentally friendly staining technology and materials will also become an important direction for the development of portable medical stainers.

[0080] A stepper motor is an electric motor that converts electrical pulse signals into corresponding angular displacement or linear displacement.

[0081] 1. Working Principle

[0082] The stepper motor works based on the principle of electromagnetism, which converts electrical energy into mechanical energy. Its operating principle is to rely on the change of air gap magnetic permeability to generate electromagnetic torque, thereby achieving rotation. When the stepper driver receives a pulse signal, it drives the stepper motor to rotate a fixed angle in the set direction. This angle is called the "step angle". By controlling the frequency and number of pulse signals, the speed and angular displacement of the stepper motor can be accurately controlled.

[0083] 2. Features

[0084] Simple control: Stepper motors are controlled by pulse signals and do not require a feedback circuit to return the position and speed information of the rotating shaft, so the control is relatively simple.

[0085] High positioning accuracy: Since the rotation of the stepper motor is discrete, each pulse signal corresponds to a fixed step angle, so high-precision positioning can be achieved.

[0086] No cumulative error: The movement of the stepper motor is carried out step by step, and the error of each step will not accumulate to the next step, so it has the characteristic of no cumulative error.

[0087] Simple structure: The structure of the stepper motor is relatively simple, easy to use and maintain, and the manufacturing cost is relatively low.

[0088] However, stepper motors also have some disadvantages, such as high noise and vibration, low efficiency, high heat generation, etc. In addition, when the control is not appropriate, synchronization loss (i.e. "out of step") may occur.

[0089] 3. Application fields

[0090] Stepper motors are widely used in many fields due to their simple control and high positioning accuracy:

[0091] Robotics and automation systems: Stepper motors can precisely control the speed and direction of robot movement, improving production efficiency and accuracy.

[0092] CNC machine tools: Stepper motors are used to control the movement of tools or workbenches to achieve high-precision processing of workpieces.

[0093] Printers: Stepper motors are used to control the movement of the print head to achieve high-quality text and image printing.

[0094] Medical equipment: For example, the movement of the scanning frame in medical imaging equipment, stepper motors can achieve fast and accurate imaging of patients.

[0095] Aerospace equipment: Stepper motors show good performance under the requirements of high precision and high stability such as satellite attitude control and rocket propulsion systems.

[0096] 4. Development Trend

[0097] With the development of microelectronics and computer technology, the control method combining software and hardware has become the mainstream of stepper motor control. The microcontroller controls the stepper motor through software, which better taps the potential of the motor. In addition, the subdivision drive technology of stepper motors has also been widely used. The subdivision drive can further reduce the step angle, improve positioning accuracy and motion stability.

[0098] The existing published patent discloses a miniature staining instrument, including a shell, a partition plate and a staining system, the partition plate is located in the shell, and the staining system is respectively connected to the shell and the partition plate; wherein the staining system includes a control module, a moving module and a glass slide processing module, the control module is connected to one side of the partition plate, and is also respectively connected to the shell, the moving module and the glass slide processing module, the moving module is located on the inner bottom wall of the shell, the glass slide processing module is respectively connected to the shell and the moving module, and the moving module and the glass slide processing module are both located on the other side of the partition plate.

[0099] By setting up a shell, the shell can adopt a hollow square structure, and the shell is divided into two spaces by a partition plate. Since the size of the partition plate is smaller than the size of the shell, the two spaces are connected and can be used to set various components in the shell. At the same time, an entrance and exit are also set on the front wall of the shell, and an airtight door is provided on the entrance and exit, which can achieve isolation from the outside world during the dyeing process and play a sealing role.

[0100] During the staining process, firstly, each module can be started to work in sequence through the set control module, and the slide processing module can be pushed out of the shell under the drive of the moving module. When the staff has placed the slide, the slide processing module can be pulled back into the shell by the moving module and put into work, so that automatic staining can be carried out. It has the advantages of small size, convenient operation, small space occupation, uniform staining effect, clean cleaning and no pollution. The whole process is completed by machine, which improves the automation and standardization of slide staining, improves work efficiency, provides convenience for staff, and is worthy of promotion.

[0101] The mobile module includes an X-axis module and a motion module. The X-axis module is connected to the inner wall of the shell; the motion module is slidably connected to the shell through a sliding member, and the sliding member is also connected to the X-axis module.

[0102] The X-axis module can be started by operating the control module, wherein the X-axis module can be in the form of a motor and a belt, and the belt is connected to a sliding part. The motor can start to drive the belt to rotate, and driven by the belt, the sliding part drives the motion module to move in the shell, wherein the sliding part can adopt a structure in which a slider and a guide rail are matched, or other structures can be adopted, which will not be elaborated here. The motion module can be in the form of a platform, located on the sliding part, and can be provided with a limiting mechanism to avoid derailment. When the motion module moves, the glass slide processing module can be pushed out of the shell, and then after the glass slide is placed, the glass slide processing module can be pulled into the shell to achieve isolation from the outside world during the staining process, and play a sealing role, thereby preventing the stain and cleaning fluid from spraying to the outside.

[0103] The slide processing module includes:

[0104] A cleaning pump and a liquid adding pump, both of which are connected to the partition plate, and solenoid valves are provided on the output ends of the cleaning pump and the liquid adding pump;

[0105] The reagent soaking box is located on the motion module, and a nozzle is provided on the reagent soaking box, the nozzle is connected to the output end of the liquid adding pump, and the reagent soaking box and the nozzle are also installed in conjunction with the cleaning pump.

[0106] By setting up the reagent immersion box, the glass slide can be placed on the reagent immersion box when the reagent immersion box is outside the shell. When the glass slide enters the shell again together with the reagent immersion box, the X-axis module is driven to simulate the manual process. The X-axis module is responsible for the forward and backward movement of the reagent immersion box and starts the liquid adding pump to draw the dye liquid (special reagent) outside the equipment to the nozzle and drip it into the reagent immersion box in turn. The liquid adding pump is provided with a solenoid valve to prevent the liquid from overflowing when the liquid adding pump is not started. After the liquid adding is completed, the liquid is soaked and left to stand for several minutes, and the cleaning pump is started to draw the cleaning liquid outside the equipment to the cleaning bin, and the nozzle and the reagent immersion box are cleaned in turn. The cleaning pump is also provided with a solenoid valve to prevent liquid overflow, and a drainage pump is also provided in the shell to discharge the waste liquid after cleaning and the waste liquid after dyeing to the waste liquid bottle outside the equipment.

[0107] The control module includes:

[0108] Switch, the switch is located on the housing;

[0109] A PCB circuit board, the PCB circuit board is connected to the partition plate and is electrically connected to the switch through a wiring block, the wiring block is located on the partition plate, and the PCB circuit board is also connected to the glass slide processing module and the moving module;

[0110] The touch screen is located on the housing and is also connected to the PCB circuit board.

[0111] The whole device can be powered on by starting the switch, and the PCB circuit board is set, wherein the PCB circuit board controls all components, implements all logic functions through programming, and works with the touch screen, wherein the touch screen adopts a small HMI touch screen, which is set on the inclined surface of the front side of the shell, and the inclination angle is suitable for human operation, so as to realize human-computer interaction;

[0112] Among them, the specific process of PCB circuit board control is:

[0113] 1. The motor control returns to zero. After completion, the X-axis module moves to the preparation position (preparation sensor position) and completes the automatic cleaning at the start-up;

[0114] 2. After the screen selection starts, the X-axis module moves to the position where the glass slide is placed outside the device;

[0115] 3. Manually insert the slide and select staining on the touch screen;

[0116] 4. The X-axis module controls the reagent immersion box to move inward, and the X-axis module returns to zero after completion;

[0117] 5. The X-axis module moves to the slide position first, and then the liquid pump works to add dye solution A;

[0118] 6. The X-axis module moves to each slide position in turn to add liquid;

[0119] 7. After adding liquid, soak and let stand for several minutes, and clean the waste dye liquid by cleaning pump;

[0120] 8. Add liquid B, liquid C and other dye solutions (special dye solutions) in sequence, and repeat steps 5, 6, and 7;

[0121] 9. Return the X-axis module to zero first, then move it to the outside of the device to take the position where the slide is placed;

[0122] 10. Manually remove the stained slides and put in new slides to be stained or click the touch screen to complete the staining, and the reagent immersion box is returned to the inside of the device;

[0123] 11. The X-axis module moves to the ready position (ready sensor position).

[0124] The micro-staining instrument also includes:

[0125] a base, the base being located on the bottom of the housing;

[0126] A through hole is formed on the side wall of the shell.

[0127] A base is provided, wherein the bottom of the base is provided with anti-slip feet, so as to facilitate the movement and placement of the entire device, and the through holes provided can be used to maintain air circulation and heat dissipation inside the device, or to connect the wires of external devices, which will not be elaborated here.

[0128] Another patent disclosed in the public disclosure discloses a special dyeing micro dyeing machine, including a casing, a base, an operating table, a control screen, a heat dissipation window, a power switch, a PTC heating sheet, a heat dissipation structure, a heating device, a control screen, a controller, a temperature sensor, a buzzer, a dyeing tank and a dyeing vat. The casing is buckled and arranged on the upper part of the base, the operating table is arranged directly above the casing, the control screen is arranged on the front elevation of the casing, and a power switch is arranged on one side of the casing. A heating device is arranged inside the casing, and the heating device is installed on the base. The heating device is an automatic constant temperature PTC heater, which is composed of a PTC heating sheet and a heat dissipation structure. The heat dissipation structure is wrapped around the outside of the PTC heating sheet. The PTC heating sheet is a commercially available PTC heating sheet, which can automatically maintain a constant temperature and can set a constant temperature. Heat dissipation windows are arranged on both sides of the casing. The operating table is arranged directly above the heating device, and at least one dyeing trough is arranged on the operating table, and a dyeing vat is arranged in the dyeing trough. The heating device is provided with rectangular grooves corresponding to the dyeing troughs one by one, and the dyeing troughs are embedded in the rectangular grooves; a controller is arranged between the heating device and the control screen, a temperature sensor is arranged on one side of the heating device, and a buzzer is installed on the base. When the temperature is abnormal or the set time is terminated, the buzzer automatically emits a warning sound.

[0129] The control panel, the heating device, the power switch, the buzzer and the temperature sensor are all electrically connected to the controller; the control panel adopts a touch screen, the dyeing tank adopts a dyeing tank made of stainless steel, and the dyeing jar adopts a dyeing jar made of PP material.

[0130] Here’s how to use it:

[0131] Add fixative, acid hydrolysis solution, DNA special staining solution and distilled water into different staining jars respectively, turn on the machine, click the control screen to set the temperature to 55°C, as well as the fixation, acid hydrolysis, staining and rinsing times.

[0132] After the temperature reaches 55°C, place the slices in the fixative staining jar for 2-5 minutes. When the warning sound sounds, take them out and wash them with running water.

[0133] Then put it into the acid lysis solution staining tank for 1-5 minutes, the warning sound is heard, take it out and wash it with running water, then put it into the DNA special staining solution for 8-15 minutes, the warning sound is heard, take it out and wash it with running water, then put it into distilled water for rinse 1-5 minutes, the warning sound is heard, take it out for dehydration and sealing.

[0134] The application has the characteristics of automatic constant temperature, safety and energy saving, corrosion resistance, long life, stability and convenience.

[0135] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the present invention is further described in detail below with reference to the accompanying drawings and specific embodiments.

[0136] Reference Figure 1-11 As shown, this embodiment provides a miniature dyeing device, including:

[0137] A protective housing 1, wherein a control module 35 for controlling the operation of the device is arranged in the protective housing 1;

[0138] A pull-out plate 2, which can be pulled out and arranged in the protective housing 1, and a glass slide 7 for carrying the tissue to be stained is positioned and arranged in the pull-out plate 2;

[0139] The positioning platform 3 is rotatably arranged on the protective shell 1, and a rotating head 6 is arranged on the protective shell 1 to be lifted and rotated corresponding to the positioning platform 3;

[0140] The consumables box 4 is positioned and installed on the positioning platform 3, the rotating head 6 is connected to the consumables box 4 by transmission, and a plurality of containers 8 for containing dyeing consumables are arranged in the consumables box 4;

[0141] The consumable pressing head 5 corresponds to the tissue on the glass slide 7 from top to bottom and is used to press the container 8 to quantitatively supply the staining consumables to the tissue.

[0142] The present invention discloses a miniature staining device, wherein a protective shell 1 is used for counterweighting, protecting the stability of the device, protecting the internal mechanism, and providing a base for the installation of the internal structure; a glass slide 7 for carrying a tissue sample to be stained is arranged in a pull-out plate 2, and the pull-out plate 2 is pulled and arranged in the protective shell 1, which can be conveniently taken and positioned, and is flexible and convenient to use; a positioning platform 3 and a rotating head 6 are arranged correspondingly up and down, and a consumable box 4 is arranged between the positioning platform 3 and the rotating head 6, and can be rotated at a fixed angle under the drive of the two, so that the consumable box 4 fixedly positioned between the two can be rotated at a fixed angle accordingly, so that a container 8 containing staining consumables can be aligned with the sample in turn, and quantitative supply of staining consumables can be realized under the quantitative extrusion of the consumable pressure head 5, and the output of the staining consumables can be accurately controlled to improve the accuracy of staining. The present invention has a compact structure, a small device volume, is easy to carry, and is suitable for different use environments. At the same time, it can accurately control the output of the reagent, improve the accuracy of staining, reduce waste, is convenient and fast, and is simple to operate, and is suitable for laboratories, hospitals, industries and other fields.

[0143] In one embodiment of the present application, a base is provided in the protective shell 1, and an anti-skid pad and anti-skid grooves are provided at the bottom of the base for anti-skid purpose, and stability is increased by counterweight; a supporting frame is provided on the base, and the supporting frame is used to support the protective shell, thereby increasing the strength of the protective shell and improving the protective performance; at the same time, the supporting frame can also serve as the installation position of internal equipment, thereby facilitating the layout and positioning of the equipment.

[0144] In one embodiment of the present application, a first handle 12 is provided on the pull-out plate 2 to facilitate pulling the pull-out plate 2 in and out.

[0145] In one embodiment of the present application, a locking mechanism is provided between the pull-out plate 2 and the supporting frame in the protective shell 1, which can achieve stable self-locking after the pull-out plate 2 is pushed into the protective shell 1, thereby avoiding insufficient dyeing accuracy caused by shaking.

[0146] Further optimization scheme, a ventilation module 9 is provided in the protective housing 1, and the ventilation module 9 is provided below the pull-out plate 2, and is used to blow the consumable liquid on the glass slide 7 off the glass slide 7, discharge it along the drainage channel 10 provided in the protective housing 1, and store it in the waste water box 11 in the protective housing 1. The ventilation module 9 is provided below the pull-out plate 2, and when the staining consumables have been soaking the tissue sample for a certain period of time, the ventilation module 9 is started to blow the excess staining consumables on the glass slide 7 off the wave row; the drainage channel 10 is provided below the glass slide 7, and the blown-off staining consumables fall into the drainage channel 10, and are recovered in the waste water box 11 through the inclined drainage channel 10 flow channel, which is convenient for subsequent recovery and avoids pollution to the surrounding environment.

[0147] In one embodiment of the present application, the waste liquid box can be pulled out from the protective shell 1 to facilitate the treatment of the waste liquid inside.

[0148] In one embodiment of the present application, a second handle 13 is provided at one end of the waste liquid box outside the protective shell 1 to facilitate pulling the waste liquid box in and out.

[0149] In one embodiment of the present application, a locking mechanism is provided between the waste liquid box and the supporting frame of the protective shell 1, which can achieve stable self-locking after the waste liquid box is pushed into the protective shell 1, thereby preventing waste liquid from spilling due to shaking of the waste liquid box.

[0150] According to a further optimization scheme, a working platform 38 is provided on the protective housing 1, and a positioning groove 14 is provided on the working platform 38. The positioning platform 3 is rotatably connected in the positioning groove 14, and the positioning groove 14 is adapted to the consumable box 4. During installation, the consumable box 4 slides into the inner wall of the positioning groove 14 and is positioned on the positioning platform 3. One side of the protective housing 1 is concave to form the working platform 38, and the positioning groove 14 is provided through the bottom surface of the working platform, and the positioning platform 3 is rotatably connected in the positioning groove 14. The glass slide 7 and the drainage channel 10 are located below the positioning groove 14. When the consumable box 4 is installed on the positioning platform 3, the consumable pressing head 5 squeezes the container 8, so that the dyeing consumables fall into the container 8 below; and the positioning groove 14 is adapted to the consumable box 4. During installation, the consumable box 4 slides along the positioning groove 14 onto the positioning platform 3 for easy positioning.

[0151] Further optimization scheme, a guide block 15 is arranged in the positioning groove 14, and a plurality of limit grooves 16 are arranged on the guide block 15. The limit grooves 16 are arranged corresponding to the through grooves 17 arranged on the positioning platform 3. The outlet of the container 8 extends out of the consumable box 4 and the limit grooves 16 and the through grooves 17 are arranged correspondingly. A guide block 15 is installed in the positioning groove 14, and a plurality of limit grooves 16 are arranged on the guide block 15 to match the outlet of the container 8; and one end of the through groove 17 on the positioning platform 3 penetrates the side wall and is connected to the limit groove 16; when the consumable box 4 is installed, the outlet of the container 8 slides along the limit groove 16 and slides into the through groove 17, which is convenient for positioning and ensures the accuracy of installation.

[0152] In one embodiment of the present application, a guide tube 36 is provided under the positioning table 3, and the guide tube 36 corresponds to the consumable press head 5 up and down. When working, the container 8 corresponding to the consumable press head 5 corresponds to the guide tube 36, so that the squeezed staining consumable falls onto the glass slide 7 along the guide tube 36, avoiding the inaccuracy caused by the scattering of the staining consumable.

[0153] In a further optimized solution, a plurality of filling slots 18 for filling the container 8 are provided in the consumable box 4, and a liquid outlet 19 is provided at the bottom end of the container 8, and the liquid outlet 19 extends out of the filling slots 18. The filling slots 18 in the consumable box 4 are arranged circumferentially, and the bottom end of the filling slots 18 extends out of the consumable box 4 through the liquid outlet 19. The container 8 is inverted in the filling slots 18. When the container 8 is squeezed, the dyeing consumables in the container 8 flow from the outlet into the liquid outlet 19, and fall from the liquid outlet 19 into the guide tube 36 below, and flow onto the glass slide 7.

[0154] In one embodiment of the present application, two control balls 20 in contact with each other are disposed in the liquid outlet 19 , and the opening and closing of the liquid outlet 19 can be controlled by pressing, so as to conveniently and accurately control the discharge amount of the dyeing consumables.

[0155] A further optimized solution is that a clearance groove 21 and a fixing card 22 are provided at the top of the consumable box 4. The clearance groove 21 is provided on the side wall of the consumable box 4 and corresponds to the rotating head 6. The fixing card 22 is opened on one side of the clearance groove 21 and is fixed at the top of the consumable box 4. When the consumable box 4 is installed, the rotating head 6 passes through the clearance groove 21 and is clamped in the fixing card 22. The give-way groove 21 is arranged on the side wall of the consumable box 4, and the one-way opening fixed card 22 is fixed at the center position of the consumable box 4, and its opening faces the give-way groove 21; the width of the give-way groove 21 is set corresponding to the rotating head 6. During installation, the give-way groove 21 of the consumable box 4 is facing the rotating head 6, and the liquid outlet 19 at its bottom is aligned with the limit groove 16, and then slides toward the positioning platform 3. The rotating head 6 passes through the give-way groove 21, and then is inserted into the fixed card 22 through the opening of the fixed card 22 to realize the positioning of the fixed card 22 and the rotating head 6. A prismatic structure is set at the contact position between the fixed card 22 and the rotating head 6, so that the rotating head 6 can drive the consumable box 4 to rotate accurately, so that a plurality of containers 8 are aligned with the guide tube 36 in sequence.

[0156] In one embodiment of the present application, the two arms at the opening of the fixing card 22 are made of elastic material and are in a loose state, which can ensure that the rotating head 6 can be inserted into the fixing shell from the opening.

[0157] In one embodiment of the present application, a third handle 23 is disposed on the side wall of the consumable box 4 and is opposite to the clearance groove 21 , so as to facilitate the control of the consumable box 4 .

[0158] According to a further optimized solution, a pressing motor 24 is provided in the protective housing 1, and the output shaft of the pressing motor 24 is connected to a driving gear 25, and the driving gear 25 is meshed with a longitudinally arranged driven rod 26, and the consumable pressing head 5 is fixedly installed at the bottom end of the driven rod 26, driving the consumable pressing head 5 to squeeze the container 8. When pressing, the pressing motor 24 is started to drive the driving gear 25 to rotate, thereby controlling the lifting and lowering of the driven rod 26, driving the consumable pressing head 5 fixed at the bottom end of the driven rod to lift and lower, and realizing the squeezing of the container 8.

[0159] In one embodiment of the present application, the pressing motor 24 can be a model with forward and reverse rotation functions and fixed-angle rotation, such as a stepping motor, which can realize the lifting and lowering control of the consumable pressing head 5 with high control accuracy.

[0160] Further optimization scheme, a guide cylinder 27 corresponding to the driven rod 26 is provided in the protective housing 1, and the driven rod 26 is slidably connected in the guide cylinder 27; a longitudinally arranged driven rack 28 is provided on the driven rod 26, and the driven rack 28 is exposed from the exposed groove of the side wall of the guide cylinder 27, and the driving gear 25 extends into the exposed groove and meshes with the driven rack 28 for transmission. The guide cylinder 27 is longitudinally arranged in the protective housing 1, and the driven rod 26 is slidably connected in the guide cylinder 27 to ensure the stability of the driven rod 26 and improve the accuracy; and a longitudinal driven rack 28 is provided on one side where the gap between the driven rod 26 and the guide cylinder 27 is set, and the driven rack 28 is exposed to the outside through the exposed groove of the side wall of the guide cylinder 27, so that the driven gear meshes with the driven rack 28, which is convenient for transmission.

[0161] In a further optimized solution, a rotating shaft 29 is provided on the protective housing 1, the top of the rotating shaft 29 is connected to a rotating motor 30 fixedly installed in the protective housing 1, and the bottom of the rotating shaft 29 is fixedly connected to the consumable pressing head 5. The rotating motor 30 drives the rotating shaft 29 to rotate, and the bottom end of the rotating shaft 29 is fixed to the top of the rotating head 6, thereby driving the rotating shaft 29 to rotate, and driving the consumable box 4 to rotate.

[0162] In one embodiment of the present application, the rotating motor 30 is selected to have a fixed-angle rotation function, such as a stepping motor, which can realize the fixed-angle rotation of the consumable box 4.

[0163] Further optimization scheme, a lifting bearing 31 is provided in the protective shell 1, the outer ring of the lifting bearing 31 slides longitudinally with the protective shell 1, and the rotating shaft 29 passes through the lifting bearing 31 and is fixed with the inner ring of the lifting bearing 31. A lifting bearing 31 is provided in the protective shell 1, and the outer ring of the lifting bearing 31 slides longitudinally with the supporting frame of the inner cavity of the protective shell 1, and no relative rotation occurs, and the inner ring hoop of the lifting shaft is fixed to the outer wall of the rotating shaft 29, so that the rotation and lifting of the lifting shaft will not interfere with each other.

[0164] In one embodiment of the present application, a transmission hole is provided at the top of the rotating shaft 29, and the output shaft of the rotating motor 30 extends into the transmission hole to drive the rotating shaft 29 to rotate, while also ensuring the stability of the transmission of the rotating shaft 29 when it is lifted or lowered.

[0165] In one embodiment of the present application, a baffle plate 32 is provided on the protective housing 1 corresponding to the upper and lower positioning platform 3, and the rotating head 6 and the consumable pressing head 5 are both located at the position of the baffle plate 32 to facilitate subsequent maintenance.

[0166] In one embodiment of the present application, the control module 35 has programming and program setting functions for controlling the operation of the device so that the device operates according to the set program.

[0167] In one embodiment of the present application, a first button 33 and a second button 34 are provided on the protective shell 1, and the first button 33 and the second button 34 are respectively electrically connected to the control module 35, and are used to input instructions to the control module 35 so that the control module 35 runs programs with different functions, corresponding to two different dyeing processes, for easy control.

[0168] In one embodiment of the present application, a power supply module 37 is provided in the protective housing 1 for supplying power for the operation of the device.

[0169] In one embodiment of the present application, the power supply module 37 uses a battery with a charging function, which can conveniently provide power and can be used in an environment where direct power supply is not possible, thereby improving convenience.

[0170] In one embodiment of the present application, the device can also be powered by an adapter and is suitable for different usage environments.

[0171] Operation process:

[0172] 1. Place the glass slide 7 on the pull-out plate 2 and insert it into the device;

[0173] 2. Insert the container 8 containing dyeing consumables into the consumable box 4, and then insert the consumable box 4 into the device;

[0174] 3. The consumable box 4 has a clearance groove 21 and a fixed card 22 structure, forming a fool-proof positioning structure; when inserting, the clearance groove 21 is placed corresponding to the side of the rotating head 6, and the fixed card 22 is completely inserted into the rotating head 6 through the shape and elastic deformation. This structure ensures that the consumable box 4 can be placed in the placement position according to the accurate position, that is, if any of the above conditions is not met, it cannot be put in place, thus ensuring the accuracy of dyeing;

[0175] 4. According to dyeing requirements, press the first button 33 or the second button 34, corresponding to two different dyeing processes;

[0176] 5. According to the setting, the consumable pressing head 5 is controlled to move downward by pressing the motor 24, squeezing a container 8 in the consumable box 4, so that the dyeing consumable in the container 8 is discharged from the liquid outlet 19 and dripped onto the glass slide 7;

[0177] 6. Then start the ventilation module 9 to blow away the reagent on the slide 7 and let it flow into the waste liquid box from the drainage channel 10;

[0178] 7. According to the setting, the rotary head 6 driven directly by the rotary motor 30 selects the consumable box 4 to the next container 8 station;

[0179] 8. Repeat steps 5-7;

[0180] 9. Until the entire dyeing process is completed;

[0181] 10. Pull out the pull-out plate 2 and take out the glass slide 7 to prepare for subsequent operations;

[0182] 11 Pull out the waste liquid box and pour out the waste water.

[0183] In the description of the present invention, it should be understood that the terms "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside" and "outside" etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0184] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A miniature dyeing device, characterized in that: include: A protective housing (1), wherein a control module (35) for controlling the operation of the device is arranged inside the protective housing (1); A pull-out plate (2), the pull-out plate (2) being drawable and arranged in the protective housing (1), and a glass slide (7) for carrying the tissue to be stained being positioned and arranged in the pull-out plate (2); A positioning platform (3), the positioning platform (3) being rotatably arranged on the protective shell (1), and a rotating head (6) being arranged up and down corresponding to the positioning platform (3) on the protective shell (1) so as to be lifted and rotated; A consumables box (4), the consumables box (4) is positioned and mounted on the positioning platform (3), the rotating head (6) is drivingly connected to the consumables box (4), and a plurality of containers (8) for containing dyeing consumables are arranged in the consumables box (4); A consumables pressing head (5), the consumables pressing head (5) corresponds to the tissue on the glass slide (7) above and below, and is used to press the container (8) to quantitatively supply the staining consumables to the tissue.

2. The micro dyeing device according to claim 1, characterized in that: A ventilation module (9) is provided in the protective shell (1), and the ventilation module (9) is arranged below the pull-out plate (2) and is used to blow the consumable liquid on the glass slide (7) off the glass slide (7), discharge it along a drainage channel (10) provided in the protective shell (1), and store it in a waste water box (11) in the protective shell (1).

3. The micro dyeing device according to claim 1 is characterized in that: A working platform (38) is provided on the protective shell (1), and a positioning groove (14) is provided on the working platform (38). The positioning platform (3) is rotatably connected in the positioning groove (14), and the positioning groove (14) is adapted to the consumable box (4). During installation, the consumable box (4) slides into the inner wall of the positioning groove (14) and is positioned on the positioning platform (3).

4. The micro dyeing device according to claim 3 is characterized in that: A guide block (15) is arranged in the positioning groove (14), and a plurality of limit grooves (16) are arranged on the guide block (15). The limit grooves (16) are arranged correspondingly to the through grooves (17) arranged through the positioning platform (3). The outlet of the container (8) extends out of the consumable box (4) and the limit grooves (16) and the through grooves (17) are arranged correspondingly.

5. The miniature dyeing device according to claim 1 is characterized in that: The consumables box (4) is provided with a plurality of filling slots (18) for filling the container (8), and the bottom end of the container (8) is provided with a liquid outlet (19), and the liquid outlet (19) extends out of the filling slot (18).

6. The miniature dyeing device according to claim 5, characterized in that: The top of the consumable box (4) is provided with a clearance groove (21) and a fixing card (22); the clearance groove (21) is provided on the side wall of the consumable box (4) and is provided corresponding to the rotating head (6); the fixing card (22) is opened on one side of the clearance groove (21) and is fixed to the top of the consumable box (4); when the consumable box (4) is installed, the rotating head (6) passes through the clearance groove (21) and is clamped in the fixing card (22).

7. The miniature dyeing device according to claim 1 is characterized in that: A pressing motor (24) is arranged in the protective housing (1); the output shaft of the pressing motor (24) is connected to a driving gear (25); the driving gear (25) is meshed with a longitudinally arranged driven rod (26); the consumable pressing head (5) is fixedly mounted on the bottom end of the driven rod (26) to drive the consumable pressing head (5) to squeeze the container (8).

8. The miniature dyeing device according to claim 7, characterized in that: A guide cylinder (27) is arranged in the protective housing (1) and is arranged corresponding to the driven rod (26). The driven rod (26) is slidably connected in the guide cylinder (27). A longitudinally arranged driven rack (28) is arranged on the driven rod (26). The driven rack (28) is exposed from an exposed groove on the side wall of the guide cylinder (27). The driving gear (25) extends into the exposed groove and meshes with the driven rack (28) for transmission.

9. The miniature dyeing device according to claim 1, characterized in that: A rotating shaft (29) is provided on the protective shell (1), the top end of the rotating shaft (29) is drivingly connected to a rotating motor (30) fixedly installed in the protective shell (1), and the bottom end of the rotating shaft (29) is fixedly connected to the consumable pressing head (5).

10. The miniature dyeing device according to claim 9, characterized in that: A lifting bearing (31) is arranged inside the protective shell (1), the outer ring of the lifting bearing (31) slides longitudinally with the protective shell (1), and the rotating shaft (29) passes through the lifting bearing (31) and is fixedly connected to the inner ring of the lifting bearing (31).

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