A miniature dyeing device

Abstract

The present invention belongs to the technical field of dyeing instruments and discloses a miniature dyeing device, comprising a protective housing, a control module for controlling the operation of the device disposed within the protective housing; a pull-out plate that can be pulled out of the protective housing, a glass slide for carrying tissue to be stained positioned within the pull-out plate; a positioning platform rotatably disposed on the protective housing, a rotating head disposed on the protective housing that is lifted and rotated in correspondence with the positioning platform; a consumables box positioned on the positioning platform, the rotating head being transmission-connected to the consumables box, a plurality of containers for dyeing consumables disposed within the consumables box; a consumables pressing head corresponding to the tissue on the glass slide, for pressing the container to quantitatively supply the dyeing consumables to the tissue. The present invention has a compact structure, a small device size, is easy to carry, and is suitable for different use environments. At the same time, it can accurately control the output of reagents, improve the accuracy of dyeing, reduce waste, is convenient and fast, and is simple to operate, making it suitable for laboratories, hospitals, industries, and other fields.

Description

Technical Field

[0001] The invention belongs to the technical field of dyeing instruments, and in particular relates to a miniature dyeing device. Background Art

[0002] The purpose of staining is to use one or more dyes to stain tissues and intracellular structures differently, so that the internal structure of cells can be clearly observed under a microscope and correct judgment can be made.

[0003] Common staining equipment generally includes fixed stainers and portable stainers; fixed stainers are large-scale staining equipment with high staining efficiency, good staining uniformity, high degree of automation and good safety, but large-scale staining equipment is large in size, high in cost, complicated to operate and inconvenient to use; portable stainers are characterized by portability and flexibility, but the design of portable stainers is based on their portable characteristics, which will lead to uneven tissue staining and reduced staining quality, while increasing staining time, increasing reagent consumption, and reducing the low degree of automation of staining, making it difficult to meet the needs of fast, efficient and accurate staining.

[0004] Therefore, the present application designs a miniature dyeing device to solve the above technical problems. Summary of the Invention

[0005] In order to solve the above technical problems, the present invention proposes a miniature dyeing device.

[0006] To achieve the above object, the present invention provides a miniature dyeing device, comprising:

[0007] A protective housing, wherein a control module for controlling the operation of the device is disposed within the protective housing;

[0008] A pull-out plate, which can be pulled out and arranged in the protective shell, and a glass slide for carrying the tissue to be stained is positioned and arranged in the pull-out plate;

[0009] A positioning platform, the positioning platform is rotatably arranged on the protective shell, and a rotating head is provided on the protective shell to be lifted and rotated corresponding to the positioning platform;

[0010] A consumables box, the consumables box is positioned and mounted on the positioning platform, the rotating head is in transmission connection with the consumables box, and a plurality of containers for holding dyeing consumables are provided in the consumables box;

[0011] A consumables pressing head corresponds to the tissue on the glass slide above and below, and is used to press the container to quantitatively supply staining consumables to the tissue.

[0012] Preferably, a ventilation module is provided in the protective shell, and the ventilation module is arranged below the pull-out plate, for blowing the consumable liquid on the glass slide off the glass slide, discharging it along the drainage channel provided in the protective shell, and storing it in a waste water box in the protective shell.

[0013] Preferably, a working platform is provided on the protective shell, a positioning groove is opened on the working platform, the positioning platform is rotatably connected in the positioning groove, the positioning groove is adapted to the consumable box, and during installation, the consumable box slides into the inner wall of the positioning groove and is positioned on the positioning platform.

[0014] Preferably, a guide block is provided in the positioning groove, and a plurality of limit grooves are provided on the guide block. The limit grooves are provided corresponding to the through grooves provided on the positioning platform. The outlet of the container extends out of the consumable box and the limit grooves and the through grooves are provided correspondingly.

[0015] Preferably, a plurality of filling slots for filling the container are provided in the consumables box, and a liquid outlet is provided at the bottom end of the container, and the liquid outlet extends out of the filling slots.

[0016] Preferably, a clearance groove and a fixing card are provided at the top of the consumable box. The clearance groove is provided on the side wall of the consumable box and corresponds to the rotating head. The fixing card is open to one side of the clearance groove and is fixed to the top of the consumable box. When the consumable box is installed, the rotating head passes through the clearance groove and is clamped in the fixing card.

[0017] Preferably, a pressing motor is provided in the protective shell, the output shaft of the pressing motor is connected to a driving gear, the driving gear is meshed with a longitudinally arranged driven rod, and the consumable pressing head is fixedly installed at the bottom end of the driven rod, driving the consumable pressing head to squeeze the container.

[0018] Preferably, a guide cylinder corresponding to the driven rod is provided in the protective shell, and the driven rod is slidingly connected in the guide cylinder; a longitudinally arranged driven rack is provided on the driven rod, and the driven rack is exposed from the exposed groove of the side wall of the guide cylinder, and the driving gear extends into the exposed groove and engages with the driven rack for transmission.

[0019] Preferably, a rotating shaft is provided on the protective shell, the top end of the rotating shaft is transmission-connected to a rotating motor fixedly installed in the protective shell, and the bottom end of the rotating shaft is fixedly connected to the consumable pressing head.

[0020] Preferably, a lifting bearing is provided in the protective shell, the outer ring of the lifting bearing slides longitudinally with the protective shell, and the rotating shaft passes through the lifting bearing and is fixedly connected to the inner ring of the lifting bearing.

[0021] Compared with the prior art, the present invention has the following advantages and technical effects: the present invention discloses a miniature staining device, the protective shell is used for counterweighting, protecting the stability of the device, protecting the internal mechanism, and providing a base for the installation of the internal structure; the glass slide for carrying the tissue sample to be stained is arranged in a pull-out plate, and the pull-out plate is pulled out and arranged in the protective shell, which can be conveniently taken out and positioned, and is flexible and convenient to use; the positioning table and the rotating head are correspondingly arranged up and down, and the consumable box is arranged between the positioning table and the rotating head, and can be rotated at a fixed angle under the drive of the two, so that the consumable box positioned and fixed between the two can rotate at a fixed angle accordingly, so that the container containing the staining consumables can be aligned with the sample in turn, and the quantitative supply of the staining consumables is realized under the quantitative squeezing of the consumable pressure head, and the output of the staining consumables is accurately controlled to improve the accuracy of staining.

[0022] The present invention has a compact structure, a small device size, is easy to carry, and is suitable for different usage environments. At the same time, it can accurately control the output of the reagent, improve the accuracy of dyeing, reduce waste, is convenient and fast, and is simple to operate. It is suitable for laboratories, hospitals, industries and other fields. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] The accompanying drawings, which constitute part of this application, are intended to provide a further understanding of this application. The exemplary embodiments and descriptions of this application are intended to explain this application and do not constitute an improper limitation on this application. In the accompanying drawings:

[0024] Figure 1 This is a schematic diagram of the miniature dyeing equipment pull-out plate in the unfolded state of the present invention;

[0025] Figure 2 This is a schematic diagram of the closed state of the drawer plate of the miniature dyeing equipment of the present invention;

[0026] Figure 3 This is an axial view of the internal structure of the miniature dyeing device of the present invention;

[0027] Figure 4 This is a schematic cross-sectional view of the micro-dyeing device structure of the present invention;

[0028] Figure 5 This is a schematic structural diagram of the positioning platform of the present invention;

[0029] Figure 6 This is a schematic diagram of the shielding plate structure of the present invention;

[0030] Figure 7 This is a view of the motor shaft pressed by the present invention;

[0031] Figure 8 This is an axial view of the consumable box of the present invention;

[0032] Figure 9A top view of the consumables box of the present invention;

[0033] Figure 10 For the present invention Figure 9 Schematic cross-section of the middle AA;

[0034] Figure 11 For the present invention Figure 9 Schematic cross-section of the middle BB;

[0035] In the figure: 1. Protective shell; 2. Pull-out plate; 3. Positioning table; 4. Consumables box; 5. Consumables pressure head; 6. Rotating head; 7. Glass slide; 8. Container; 9. Ventilation module; 10. Drain channel; 11. Waste water box; 12. First handle; 13. Second handle; 14. Positioning groove; 15. Guide block; 16. Limiting groove; 17. Through groove; 18. Filling groove; 19. Liquid outlet; 20. Control ball; 21. Yield groove; 22. Fixing card; 23. Third handle; 24. Pressing motor; 25. Driving gear; 26. Driven rod; 27. Guide cylinder; 28. Driven rack; 29. ​​Rotating shaft; 30. Rotating motor; 31. Lifting bearing; 32. Shielding plate; 33. First button; 34. Second button; 35. Control module; 36. Diversion pipe; 37. Power supply module. DETAILED DESCRIPTION

[0036] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0037] Dyeing equipment

[0038] In the medical system, staining technology is a vital technology. It is widely used in pathology, histology, cytology and other fields, providing an important basis for the diagnosis and treatment of diseases.

[0039] 1. Purpose and principle of dyeing

[0040] The main purpose of staining is to improve the resolution of specimen components under an optical microscope, making cells and tissue structures more clearly visible. The principle is to use dyes to chemically react or physically adsorb with certain components in cells or tissues, thereby changing the refractive index of these components to light, causing them to appear different colors under the microscope.

[0041] 2. Commonly used dyeing methods

[0042] HE staining, also known as hematoxylin-eosin staining, is one of the most commonly used staining methods in histology. Hematoxylin primarily stains cell nuclei, giving them a blue or purple appearance, while eosin stains the cytoplasm and other tissue structures, giving them a pink appearance. HE staining is simple and easy to perform, providing information on cell morphology and tissue structure. It is fundamental to pathological diagnosis, assessment of tissue damage, and determination of tumor type.

[0043] Special stains: These stains, also known as histochemical stains, offer a richer range of colors and can directly reveal specific chemical substances inside and outside cells. For example, reticular fiber stains are used to differentiate between carcinomas and sarcomas; elastic fiber stains are used to determine whether lung cancer has invaded the pleura; acid-fast stains are used to identify tuberculosis bacteria in tissues; and PAS stains are used for the diagnosis and differential diagnosis of fungal infections. Special stains can reveal cellular structures or tissue components not visible with conventional stains, aiding in the identification of tissue or cell lesions and providing more accurate information for diagnosis and treatment.

[0044] Immunohistochemical staining utilizes the specific binding between antigens and antibodies to extract a chemical substance from tissues or cells as an antigen. Specific antibodies are then obtained by immunizing animals, and these antibodies are then used to detect similar antigenic substances in tissues or cells. Immunohistochemical staining can provide information on specific proteins, including their expression and localization, and is crucial for studying gene expression, cell differentiation, and tumorigenesis.

[0045] 3. Dyeing process and precautions

[0046] Process: Staining typically involves dehydration, humidification, sectioning, spreading, scooping, staining, dehydration, transparency, and sealing. The quality of the sections significantly impacts the staining results, so ensure the blade is flat and sharp before slicing. When slicing, ensure that the tissue layers and texture are parallel to the knife.

[0047] Note: During the staining process, it is necessary to pay attention to the dye concentration, staining time, temperature and other conditions to ensure the accuracy and stability of the staining results. At the same time, for special samples or experimental requirements, special staining methods or adjustments to the staining conditions may be required.

[0048] 4. Application and development of dyeing

[0049] Applications: Staining technology has a wide range of applications in the medical system. It is not only used for the diagnosis and differential diagnosis of diseases, but also for assessing the degree of tissue damage, determining tumor types, and studying cell differentiation. In addition, with the continuous development of medical technology, staining technology is constantly expanding into new application areas.

[0050] Development: In recent years, with the rapid development of disciplines such as molecular biology and genetics, staining techniques have also been continuously innovated and improved. For example, technologies such as fluorescence in situ hybridization (FISH) and gene chips have been gradually applied to clinical pathology diagnosis, providing more accurate and reliable means for disease diagnosis and treatment.

[0051] Advantages and disadvantages of large-scale dyeing equipment:

[0052] advantage

[0053] High throughput: Large-scale medical staining equipment can usually process multiple samples simultaneously, significantly improving staining efficiency. For laboratories that need to process large-scale samples, this can greatly shorten the staining cycle and improve overall work efficiency.

[0054] Good staining uniformity: These devices typically utilize advanced staining technology and control systems to ensure uniform heating and staining of samples during the staining process, resulting in uniform and consistent staining results. This helps improve diagnostic accuracy and reliability.

[0055] High degree of automation: Large medical staining equipment is often equipped with automated control systems that automate the entire process from sample loading, staining, rinsing, to drying. This reduces the complexity of manual operations, reduces human errors, and improves the stability and repeatability of staining quality.

[0056] Versatility: Some high-end, large-scale staining equipment also has multiple staining modes and functions to meet different sample types and staining needs; this makes the equipment more flexible and adaptable, and can meet the diverse needs of different laboratories.

[0057] High safety: Large medical staining equipment is usually equipped with comprehensive safety systems, such as overheat protection, leakage protection, and emergency shutdown functions, to ensure the safety and stability of the staining process. This helps protect the safety of operators while preventing equipment damage and sample contamination.

[0058] shortcoming

[0059] High Cost: Large-scale medical staining equipment is often expensive, which can be a burden for laboratories with limited budgets. Furthermore, equipment maintenance and repair costs can be high, requiring additional investment of funds and resources.

[0060] Complex operation: Although the equipment is highly automated, operation and maintenance still require certain professional knowledge and skills. For operators who lack relevant experience, it may take a long time of training and practice to master the operation and maintenance methods of the equipment.

[0061] Large space requirement: Large medical staining equipment is usually large in size and requires a large laboratory space. This can be a challenge for laboratories with limited space, requiring rational planning of laboratory layout and equipment placement.

[0062] High reagent consumption: Although the equipment has efficient staining capabilities, it also consumes a large amount of reagents such as stains and rinse solutions; this increases experimental costs and may cause certain environmental pollution. Therefore, it is necessary to use reagents rationally and dispose of waste properly.

[0063] Advantages and disadvantages of portable dyeing equipment;

[0064] A portable medical stainer is a staining device specially designed for use in the medical field. It is characterized by portability and flexibility, and can meet staining needs in different scenarios.

[0065] 1. Main Features

[0066] Portability: The portable medical stainer is small in size and light in weight, easy to carry and move, and can adapt to the staining needs of different places, such as field medical rescue, mobile laboratories, etc.

[0067] Flexibility: The device usually has a variety of staining modes and functions, which can be adjusted and set according to different sample types and staining requirements to achieve personalized staining plans.

[0068] Degree of automation: Although they are portable devices, many portable medical stainers still have a certain degree of automation functions, such as automatic sample addition, automatic staining, automatic rinsing, etc., which reduces the complexity of manual operation.

[0069] Accuracy: The portable medical stainer adopts advanced staining technology and control system, which can ensure the accuracy and consistency of staining results and improve the reliability of diagnosis.

[0070] 2. Application Scenarios

[0071] Field medical rescue: In emergency situations such as natural disasters and wars, portable medical stainers can quickly provide staining services to support the diagnosis and treatment of the wounded and sick.

[0072] Mobile laboratories: In research, teaching, or disease monitoring projects, mobile laboratories may need to change locations frequently. The portability and flexibility of portable medical stainers make them an ideal choice for mobile laboratories.

[0073] Primary medical institutions: In some remote areas or primary medical institutions, due to limited resources, they may not be equipped with large-scale staining equipment. Portable medical stainers can be used as an alternative to meet the staining needs of these institutions.

[0074] 3. Precautions

[0075] Operating specifications: Although the portable medical stainer has automated functions, the operator still needs to operate it according to the instructions and specifications to ensure the accuracy and safety of the staining results.

[0076] Maintenance: Clean, inspect and repair the equipment regularly to ensure normal operation of the equipment and extend its service life.

[0077] Reagent management: Rationally use and manage dyes, flushing solutions and other reagents to avoid waste and pollution.

[0078] 4. Market Trends

[0079] With the continuous advancement of medical technology and people's increasing demand for health, the market demand for portable medical stainers is also increasing. In the future, portable medical stainers will become more intelligent, automated, and multifunctional to meet more diverse staining needs. At the same time, with the increasing awareness of environmental protection, green and environmentally friendly staining technologies and materials will become an important development direction for portable medical stainers.

[0080] A stepper motor is an electric motor that converts electrical pulse signals into corresponding angular displacement or linear displacement.

[0081] 1. Working Principle

[0082] Stepper motors operate based on electromagnetic principles, converting electrical energy into mechanical energy. They rely on changes in the air gap's magnetic permeability to generate electromagnetic torque, thereby achieving rotation. When a stepper driver receives a pulse signal, it drives the stepper motor in a set direction and through a fixed angle, known as the "step angle." By controlling the frequency and number of pulse signals, the stepper motor's speed and angular displacement can be precisely controlled.

[0083] 2. Features

[0084] Simple control: Stepper motors are controlled by pulse signals and do not require a feedback circuit to return the position and speed information of the rotating shaft, so the control is relatively simple.

[0085] High positioning accuracy: Since the rotation of the stepper motor is discrete, each pulse signal corresponds to a fixed step angle, so high-precision positioning can be achieved.

[0086] No cumulative error: The movement of the stepper motor is carried out step by step, and the error of each step will not accumulate to the next step, so it has the characteristic of no cumulative error.

[0087] Simple structure: The structure of the stepper motor is relatively simple, easy to use and maintain, and the manufacturing cost is relatively low.

[0088] However, stepper motors also have some disadvantages, such as high noise and vibration, low efficiency, high heat generation, etc. In addition, when the control is not appropriate, synchronization loss (i.e. "step loss") may occur.

[0089] 3. Application Fields

[0090] Stepper motors are widely used in many fields due to their simple control and high positioning accuracy.

[0091] Robotics and automation systems: Stepper motors can precisely control the speed and direction of robot movement, improving production efficiency and accuracy.

[0092] CNC machine tools: Stepper motors are used to control the movement of tools or worktables to achieve high-precision processing of workpieces.

[0093] Printers: Stepper motors are used to control the movement of the print head to achieve high-quality text and image printing.

[0094] Medical equipment: For example, the movement of the scanning frame in medical imaging equipment, stepper motors can achieve fast and accurate imaging of patients.

[0095] Aerospace equipment: Stepper motors demonstrate excellent performance under the requirements of high precision and high stability in satellite attitude control, rocket propulsion systems, etc.

[0096] 4. Development Trends

[0097] With the development of microelectronics and computer technology, a combination of hardware and software has become the mainstream control method for stepper motors. Microcontrollers control stepper motors through software, further unlocking their potential. Furthermore, microstepping drive technology for stepper motors has also gained widespread application. This technology can further reduce the step angle, improving positioning accuracy and motion smoothness.

[0098] The existing published patent discloses a miniature stainer, including a shell, a partition plate and a staining system, the partition plate is located inside the shell, and the staining system is respectively connected to the shell and the partition plate; wherein, the staining system includes a control module, a moving module and a slide processing module, the control module is connected to one side of the partition plate, and is also respectively connected to the shell, the moving module and the slide processing module, the moving module is located on the inner bottom wall of the shell, the slide processing module is respectively connected to the shell and the moving module, and the moving module and the slide processing module are both located on the other side of the partition plate.

[0099] By setting up a shell, the shell can adopt a hollow square structure, and the shell is divided into two spaces by a partition plate. Since the size of the partition plate is smaller than the size of the shell, the two spaces are connected and can be used to set various components in the shell. At the same time, an entrance and exit are also set on the front wall of the shell, and an airtight door is provided on the entrance and exit, which can achieve isolation from the outside world during the dyeing process and play a sealing role.

[0100] During the staining process, first, through the control module, each module can be started to work in sequence. Driven by the mobile module, the slide processing module can be pushed out of the shell. When the staff has placed the slide, the mobile module can be pulled again to make the slide processing module re-enter the shell and put into work, so that automatic staining can be carried out. It is compact, easy to operate, occupies little space, has uniform staining effect, is clean and pollution-free, and the entire process is completed by machinery, which improves the automation and standardization of slide staining, and improves work efficiency, provides convenience for staff, and is worthy of promotion.

[0101] The mobile module includes an X-axis module and a motion module. The X-axis module is connected to the inner wall of the shell; the motion module is slidably connected to the shell through a sliding member, and the sliding member is also connected to the X-axis module.

[0102] The X-axis module can be started by operating the control module, wherein the X-axis module can be in the form of a motor and a belt, and the belt is connected to the sliding part. The motor can drive the belt to rotate when it is started. Driven by the belt, the sliding part drives the motion module to move in the shell, wherein the sliding part can adopt a structure in which a slider and a guide rail are matched, or other structures can be adopted. No further details will be given here. The motion module can be in the form of a platform, located on the sliding part, and can be provided with a limiting mechanism to avoid derailment. When the motion module moves, the glass slide processing module can be pushed out of the shell, and then after the glass slide is placed, the glass slide processing module can be pulled into the shell to achieve isolation from the outside world during the staining process, which plays a sealing role, thereby preventing the dye and cleaning fluid from spraying to the outside.

[0103] The slide processing module includes:

[0104] A cleaning pump and a liquid adding pump are both connected to the partition plate, and electromagnetic valves are provided on the output ends of the cleaning pump and the liquid adding pump;

[0105] The reagent soaking box is located on the motion module and is provided with a nozzle, which is connected to the output end of the liquid adding pump. The reagent soaking box and the nozzle are also installed in conjunction with the cleaning pump.

[0106] By setting up a reagent soaking box, the glass slide can be placed on the reagent soaking box when the reagent soaking box is outside the shell. When the glass slide enters the shell again along with the reagent soaking box, the X-axis module is driven to simulate the manual process. The X-axis module is responsible for the forward and backward movement of the reagent soaking box and starts the liquid adding pump to draw the dye liquid (special reagent) outside the equipment to the nozzle and drip it into the reagent soaking box in turn. The liquid adding pump is provided with a solenoid valve to prevent the liquid from overflowing when the liquid adding pump is not started. After the liquid adding is completed, the soaking is allowed to stand for a few minutes, and the cleaning pump is started to draw the cleaning liquid outside the equipment to the cleaning bin, and the nozzle and the reagent soaking box are cleaned in turn. The cleaning pump is also provided with a solenoid valve to prevent liquid overflow, and a drainage pump is also provided in the shell to discharge the waste liquid after cleaning and the waste liquid after dyeing to the waste liquid bottle outside the equipment.

[0107] The control module includes:

[0108] Switch, the switch is located on the housing;

[0109] A PCB circuit board, the PCB circuit board is connected to the partition plate and is electrically connected to the switch through a terminal block, the terminal block is located on the partition plate, and the PCB circuit board is also connected to the slide processing module and the moving module;

[0110] The touch screen is located on the housing and is also connected to the PCB circuit board.

[0111] The entire device can be powered on by starting the switch, and the PCB circuit board is set. The PCB circuit board controls all components, implements all logic functions through programming, and works with the touch screen. The touch screen adopts a small HMI touch screen, which is set on the inclined surface of the front side of the shell. The tilt angle is suitable for human operation, thereby realizing human-computer interaction;

[0112] Among them, the specific process of PCB circuit board control is:

[0113] 1. The motor control returns to zero. After completion, the X-axis module moves to the ready position (ready sensor position) and completes the automatic cleaning of the machine.

[0114] 2. After the screen selection starts, the X-axis module moves to the position where the slide is placed outside the device;

[0115] 3. Manually insert the slide and select staining on the touch screen;

[0116] 4. The X-axis module controls the inward movement of the reagent immersion box. After completion, the X-axis module returns to zero.

[0117] 5. The X-axis module moves to the slide position first, and then the liquid pump works to add dye solution A;

[0118] 6. The X-axis module moves to each slide position in turn to add liquid;

[0119] 7. After adding liquid, soak and let it stand for a few minutes, and clean the pump to clean the waste dye liquid;

[0120] 8. Add liquid B, liquid C and other dye solutions (special dye solutions) in sequence, and repeat steps 5, 6, and 7;

[0121] 9. Return the X-axis module to zero first, then move it to the outside of the device to take the position where the slide is placed;

[0122] 10. Manually remove the stained slide and insert a new slide to be stained or click the touch screen to complete the staining, and the reagent immersion box is returned to the inside of the device;

[0123] 11. The X-axis module moves to the ready position (ready sensor position).

[0124] The micro-stainer also includes:

[0125] a base, the base being located on the bottom of the housing;

[0126] The through hole is opened on the side wall of the shell.

[0127] A base is provided, wherein the bottom of the base is provided with non-slip feet to facilitate the movement and placement of the entire device, and the through holes provided can be used to maintain air circulation and heat dissipation inside the device, or to connect wires to external devices. I will not go into details here.

[0128] Another patent disclosed a special dyeing micro-dyeing machine, which includes a casing, a base, an operating table, a control screen, a heat dissipation window, a power switch, a PTC heating sheet, a heat dissipation structure, a heating device, a control screen, a controller, a temperature sensor, a buzzer, a dyeing tank and a dyeing vat. The casing is buckled and arranged on the upper part of the base, the operating table is arranged directly above the casing, the control screen is arranged on the front elevation of the casing, and a power switch is arranged on one side of the casing. A heating device is arranged inside the casing and is mounted on the base. The heating device is an automatic constant temperature PTC heater, which consists of a PTC heating sheet and a heat dissipation structure. The heat dissipation structure is wrapped around the outside of the PTC heating sheet. The PTC heating sheet is a commercially available PTC heating sheet that can automatically maintain a constant temperature and can set a constant temperature. Heat dissipation windows are arranged on both sides of the casing. The operating table is arranged directly above the heating device, and at least one dyeing trough is arranged on the operating table, and a dyeing vat is arranged in the dyeing trough. The heating device is provided with rectangular grooves corresponding to the dyeing troughs one by one, and the dyeing troughs are embedded in the rectangular grooves; a controller is arranged between the heating device and the control screen, a temperature sensor is arranged on one side of the heating device, and a buzzer is installed on the base. When the temperature is abnormal or the set time is expired, the buzzer automatically emits a warning sound.

[0129] The control panel, heating device, power switch, buzzer and temperature sensor are all electrically connected to the controller; the control panel adopts a touch screen, the dyeing tank adopts a dyeing tank made of stainless steel, and the dyeing vat adopts a dyeing vat made of PP material.

[0130] Here’s how to use it:

[0131] Add fixative, acid hydrolysis solution, DNA special staining solution and distilled water into different staining jars respectively, turn on the machine, click the control screen to set the temperature to 55℃, and the fixation, acid hydrolysis, staining and rinsing times.

[0132] After the temperature reaches 55°C, place the sections in the fixative staining jar for 2-5 minutes. When the warning sound sounds, remove the sections and rinse with running water.

[0133] Then put it into the acid solution staining tank for 1-5 minutes. When the warning sound is heard, take it out and wash it with running water. Then put it into the DNA special staining solution for 8-15 minutes. When the warning sound is heard, take it out and wash it with running water. Then put it into distilled water and rinse it for 1-5 minutes. When the warning sound is heard, take it out and dehydrate it, and seal it.

[0134] The application has the characteristics of automatic constant temperature, safety and energy saving, corrosion resistance, long life, stability and convenience.

[0135] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the present invention is further described in detail below with reference to the accompanying drawings and specific embodiments.

[0136] Reference Figure 1-11 As shown, this embodiment provides a miniature dyeing device, including:

[0137] A protective housing 1, wherein a control module 35 for controlling the operation of the device is arranged in the protective housing 1;

[0138] A pull-out plate 2 is provided in the protective housing 1 and is provided with a slide 7 for supporting the tissue to be stained.

[0139] The positioning platform 3 is rotatably mounted on the protective housing 1. The protective housing 1 is provided with a rotary head 6 which is arranged up and down corresponding to the positioning platform 3;

[0140] The consumables box 4 is positioned and mounted on the positioning platform 3. The rotating head 6 is in transmission connection with the consumables box 4. The consumables box 4 contains a plurality of containers 8 for containing dyeing consumables.

[0141] The consumable pressing head 5 corresponds to the tissue on the glass slide 7 above and below, and is used to press the container 8 to quantitatively supply the staining consumables to the tissue.

[0142] The present invention discloses a miniature staining device, wherein a protective shell 1 is used for counterweighting, protecting the stability of the device, protecting the internal mechanism, and providing a base for the installation of the internal structure; a glass slide 7 for carrying a tissue sample to be stained is arranged in a pull-out plate 2, and the pull-out plate 2 is pulled out and arranged in the protective shell 1, which can be conveniently taken in and positioned, and is flexible and convenient to use; a positioning platform 3 and a rotating head 6 are correspondingly arranged up and down, and a consumables box 4 is arranged between the positioning platform 3 and the rotating head 6, and can be rotated at a fixed angle under the drive of the two, so that the consumables box 4 positioned and fixed between the two can be rotated at a fixed angle accordingly, so that the container 8 containing the staining consumables can be aligned with the sample in turn, and the quantitative supply of the staining consumables can be achieved under the quantitative squeezing of the consumables pressure head 5, accurately controlling the output of the staining consumables, and improving the accuracy of staining. The present invention has a compact structure, a small device volume, is easy to carry, is suitable for different use environments, can accurately control the output of the reagent, improve the accuracy of staining, reduce waste, is convenient and fast, is simple to operate, and is suitable for laboratories, hospitals, industries and other fields.

[0143] In one embodiment of the present application, a base is provided in the protective shell 1, and an anti-slip pad and anti-slip grooves are provided at the bottom of the base for anti-slip, and the stability is increased by counterweight; a supporting frame is provided on the base, and the supporting frame is used to support the protective shell, improve the strength of the protective shell, and improve the protective performance; at the same time, the supporting frame can also serve as the installation position of internal equipment, which facilitates the layout and positioning of the equipment.

[0144] In one embodiment of the present application, a first handle 12 is provided on the pull-out plate 2 to facilitate pulling the pull-out plate 2 in and out.

[0145] In one embodiment of the present application, a locking mechanism is provided between the pull-out plate 2 and the supporting frame in the protective shell 1, which can achieve stable self-locking after the pull-out plate 2 is pushed into the protective shell 1, thereby avoiding insufficient dyeing accuracy caused by shaking.

[0146] To further optimize the solution, a ventilation module 9 is provided in the protective housing 1. The ventilation module 9 is provided below the pull-out plate 2 and is used to blow the consumable liquid on the glass slide 7 off the glass slide 7, discharge it along the drainage channel 10 provided in the protective housing 1, and store it in the waste water box 11 in the protective housing 1. The ventilation module 9 is provided below the pull-out plate 2. After the staining consumables have been soaking the tissue sample for a certain period of time, the ventilation module 9 is started to blow the excess staining consumables on the glass slide 7 off the wave array; the drainage channel 10 is provided below the glass slide 7, and the blown-off staining consumables fall into the drainage channel 10 and are recovered in the waste water box 11 through the inclined drainage channel 10, which facilitates subsequent recycling and avoids pollution to the surrounding environment.

[0147] In one embodiment of the present application, the waste liquid box can be pulled out from the protective shell 1 to facilitate the disposal of the waste liquid inside.

[0148] In one embodiment of the present application, a second handle 13 is provided at one end of the waste liquid box outside the protective shell 1 to facilitate the pulling in and out of the waste liquid box.

[0149] In one embodiment of the present application, a locking mechanism is provided between the waste liquid box and the supporting frame of the protective shell 1, which can achieve stable self-locking after the waste liquid box is pushed into the protective shell 1, thereby avoiding waste liquid spillage caused by shaking of the waste liquid box.

[0150] A further optimized solution is provided on the protective shell 1 with a working platform 38, and a positioning groove 14 is provided on the working platform 38. The positioning platform 3 is rotatably connected to the positioning groove 14, and the positioning groove 14 is adapted to the consumables box 4. During installation, the consumables box 4 slides into the inner wall of the positioning groove 14 and is positioned on the positioning platform 3. One side of the protective shell 1 is concave to form the working platform 38. The positioning groove 14 is provided through the bottom surface of the working platform, and the positioning platform 3 is rotatably connected to the positioning groove 14. The glass slide 7 and the drainage channel 10 are located below the positioning groove 14. When the consumables box 4 is installed on the positioning platform 3, the consumables pressing head 5 squeezes the container 8, causing the staining consumables to fall into the container 8 below. The positioning groove 14 is adapted to the consumables box 4. During installation, the consumables box 4 slides along the positioning groove 14 onto the positioning platform 3 for easy positioning.

[0151] A further optimized solution is provided, in which a guide block 15 is provided within the positioning groove 14, and a plurality of limiting grooves 16 are provided on the guide block 15. The limiting grooves 16 correspond to the through-grooves 17 provided through the positioning platform 3. The outlet of the container 8 extends out of the consumables box 4, and the limiting grooves 16 and the through-grooves 17 are provided correspondingly. A guide block 15 is installed within the positioning groove 14, and a plurality of limiting grooves 16 are provided on the guide block 15 to match the outlet of the container 8. One end of the through-grooves 17 on the positioning platform 3 passes through the side wall and communicates with the limiting grooves 16. When the consumables box 4 is installed, the outlet of the container 8 slides along the limiting grooves 16 and into the through-grooves 17, facilitating positioning and ensuring accurate installation.

[0152] In one embodiment of the present application, a guide tube 36 is provided below the positioning table 3, and the guide tube 36 corresponds to the consumable press head 5 above and below. When working, the container 8 corresponding to the consumable press head 5 corresponds to the guide tube 36, so that the squeezed staining consumable falls along the guide tube 36 onto the glass slide 7, avoiding inaccuracy caused by the scattering of the staining consumable.

[0153] In a further optimized solution, the consumables box 4 is provided with a plurality of filling slots 18 for filling the containers 8. The bottom end of the container 8 is provided with a liquid outlet 19, which extends out of the filling slots 18. The filling slots 18 in the consumables box 4 are arranged circumferentially, and the bottom end of the filling slots 18 extends out of the consumables box 4 through the liquid outlet 19. The container 8 is upside down in the filling slots 18. When the container 8 is squeezed, the staining consumables in the container 8 flow from the outlet into the liquid outlet 19, and then fall from the liquid outlet 19 into the guide tube 36 below, flowing onto the glass slide 7.

[0154] In one embodiment of the present application, two control balls 20 in contact with each other are provided in the liquid outlet 19 , and the opening and closing of the liquid outlet 19 can be controlled by pressing, so as to conveniently and accurately control the discharge amount of the dyeing consumables.

[0155] A further optimized solution is provided in which a clearance groove 21 and a fixing card 22 are provided at the top of the consumable box 4. The clearance groove 21 is provided on the side wall of the consumable box 4 and corresponds to the rotating head 6. The fixing card 22 is opened on one side of the clearance groove 21 and is fixed at the top of the consumable box 4. When the consumable box 4 is installed, the rotating head 6 passes through the clearance groove 21 and is clamped in the fixing card 22. The give way groove 21 is set on the side wall of the consumable box 4, and the one-way opening fixed card 22 is fixed at the center position of the consumable box 4, and its opening faces the give way groove 21; the width of the give way groove 21 is set corresponding to the rotating head 6. During installation, the give way groove 21 of the consumable box 4 is facing the rotating head 6, and the liquid outlet 19 at its bottom end is aligned with the limit groove 16, and then slides toward the positioning platform 3. The rotating head 6 passes through the give way groove 21, and then is inserted into the fixed card 22 through the opening of the fixed card 22 to realize the positioning of the fixed card 22 and the rotating head 6. A prismatic structure is set at the contact position of the fixed card 22 and the rotating head 6, so that the rotating head 6 can drive the consumable box 4 to rotate precisely, so that a number of containers 8 are aligned with the guide tube 36 in sequence.

[0156] In one embodiment of the present application, the two arms at the opening of the fixing clamp 22 are made of elastic material and are in a loose state, which can ensure that the rotating head 6 can be inserted into the fixing shell through the opening.

[0157] In one embodiment of the present application, a third handle 23 is provided on the side wall of the consumable box 4 , opposite to the clearance groove 21 , to facilitate control of the consumable box 4 .

[0158] In a further optimized solution, a pressing motor 24 is provided in the protective housing 1. The output shaft of the pressing motor 24 is connected to a driving gear 25. The driving gear 25 is meshed with a longitudinally arranged driven rod 26. The consumable pressing head 5 is fixedly mounted at the bottom end of the driven rod 26, driving the consumable pressing head 5 to squeeze the container 8. When pressing, the pressing motor 24 is started to drive the driving gear 25 to rotate, thereby controlling the rise and fall of the driven rod 26, driving the consumable pressing head 5 fixed at the bottom end of the driven rod to rise and fall, thereby squeezing the container 8.

[0159] In one embodiment of the present application, the pressing motor 24 can be a model with forward and reverse rotation functions and fixed angle rotation, such as a stepping motor, which can realize the lifting and lowering control of the consumable pressure head 5 with high control accuracy.

[0160] A further optimized solution is provided within the protective housing 1, with a guide cylinder 27 corresponding to the driven rod 26. The driven rod 26 is slidably connected within the guide cylinder 27. A longitudinally arranged driven rack 28 is provided on the driven rod 26. The driven rack 28 is exposed through an exposed slot in the side wall of the guide cylinder 27. The driving gear 25 extends into the exposed slot and meshes with the driven rack 28 for transmission. The guide cylinder 27 is arranged longitudinally within the protective housing 1, and the driven rod 26 is slidably connected within the guide cylinder 27, ensuring the stability of the driven rod 26 and improving accuracy. A longitudinal driven rack 28 is provided on one side of the gap between the driven rod 26 and the guide cylinder 27. The driven rack 28 is exposed through the exposed slot in the side wall of the guide cylinder 27, allowing the driven gear and the driven rack 28 to mesh and facilitate transmission.

[0161] In a further optimized solution, a rotating shaft 29 is provided on the protective housing 1. The top end of the rotating shaft 29 is in transmission connection with a rotating motor 30 fixedly mounted in the protective housing 1, and the bottom end of the rotating shaft 29 is fixedly connected to the consumable pressing head 5. The rotating motor 30 drives the rotating shaft 29 to rotate, and the bottom end of the rotating shaft 29 is fixed to the top end of the rotating head 6, thereby driving the rotating shaft 29 to rotate, and the consumable box 4 to rotate.

[0162] In one embodiment of the present application, the rotary motor 30 is a model with a fixed-angle rotation function, such as a stepping motor, which can realize the fixed-angle rotation of the consumable box 4.

[0163] A further optimization scheme is provided within the protective housing 1. The outer ring of the lifting bearing 31 slides longitudinally with the protective housing 1. The rotating shaft 29 passes through the lifting bearing 31 and is fixedly connected to the inner ring of the lifting bearing 31. The lifting bearing 31 is provided within the protective housing 1. The outer ring of the lifting bearing 31 slides longitudinally with the support frame of the inner cavity of the protective housing 1 without relative rotation. The inner ring of the lifting shaft is fixed to the outer wall of the rotating shaft 29, so that the rotation and lifting movements of the lifting shaft do not interfere with each other.

[0164] In one embodiment of the present application, a transmission hole is provided at the top of the rotating shaft 29, and the output shaft of the rotating motor 30 extends into the transmission hole to drive the rotating shaft 29 to rotate, while also ensuring the stability of the transmission of the rotating shaft 29 when it is raised or lowered.

[0165] In one embodiment of the present application, a baffle plate 32 is provided on the protective housing 1 corresponding to the upper and lower positioning platform 3, and the rotating head 6 and the consumable pressing head 5 are both located at the position of the baffle plate 32 to facilitate subsequent maintenance.

[0166] In one embodiment of the present application, the control module 35 has programming and program setting functions, which is used to control the operation of the device so that the device operates according to the set program.

[0167] In one embodiment of the present application, a first button 33 and a second button 34 are provided on the protective shell 1, and the first button 33 and the second button 34 are electrically connected to the control module 35 respectively, and are used to input instructions to the control module 35 so that the control module 35 runs programs with different functions, corresponding to two different dyeing processes, for easy control.

[0168] In one embodiment of the present application, a power supply module 37 is provided in the protective housing 1 for supplying power to the device.

[0169] In one embodiment of the present application, the power supply module 37 uses a battery with a charging function, which can conveniently provide power and can be used in an environment where direct power supply is not possible, thereby improving convenience.

[0170] In one embodiment of the present application, the device can also be powered by an adapter and is suitable for different usage environments.

[0171] Operation process:

[0172] 1. Place the slide 7 on the pull-out plate 2 and insert it into the device;

[0173] 2. Insert the container 8 containing dyeing consumables into the consumables box 4, and then snap the consumables box 4 into the device;

[0174] 3. The consumables box 4 has a clearance groove 21 and a fixing clip 22 structure, forming a fool-proof positioning structure; when inserting, the clearance groove 21 is positioned corresponding to the side of the rotating head 6. Through the shape and elastic deformation of the fixing clip 22, it is completely locked into the rotating head 6. This structure ensures that the consumables box 4 can be placed in the placement position according to the accurate position. That is, if any of the above conditions is not met, it cannot be placed in place, thus ensuring the accuracy of dyeing;

[0175] 4. According to the dyeing requirements, press the first button 33 or the second button 34, corresponding to two different dyeing processes;

[0176] 5. According to the settings, the consumable pressing head 5 is controlled by pressing the motor 24 to move downward, squeezing a container 8 in the consumable box 4, so that the dyeing consumable in the container 8 is discharged from the liquid outlet 19 and drips onto the glass slide 7;

[0177] 6. Then start the ventilation module 9 to blow away the reagent on the slide 7 and flow it into the waste liquid box through the drainage channel 10;

[0178] 7. According to the settings, the rotary head 6 driven directly by the rotary motor 30 selects the consumable box 4 and loads it to the next container 8 station;

[0179] 8. Repeat steps 5-7;

[0180] 9. Until the entire dyeing process is completed;

[0181] 10. Pull out the pull-out plate 2 and take out the glass slide 7 to prepare for subsequent operations;

[0182] 11 Pull out the waste liquid box and pour out the waste water.

[0183] In the description of the present invention, it should be understood that the terms "longitudinal", "transverse", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship, are based on the orientation or position relationship shown in the accompanying drawings, and are only for the convenience of describing the present invention, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0184] The embodiments described above are merely descriptions of preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Without departing from the spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by persons skilled in the art should fall within the scope of protection defined by the claims of the present invention.

Claims

1. A miniature dyeing device, characterized in that: include: A protective housing (1), wherein a control module (35) for controlling the operation of the device is arranged in the protective housing (1); A pull-out plate (2), the pull-out plate (2) being drawable and arranged in the protective housing (1), and a glass slide (7) for carrying the tissue to be stained being positioned and arranged in the pull-out plate (2); A positioning platform (3), the positioning platform (3) being rotatably mounted on the protective housing (1), and a rotating head (6) being arranged on the protective housing (1) so as to be lifted and rotated and corresponding to the positioning platform (3) above and below; A consumables box (4), the consumables box (4) is positioned and mounted on the positioning platform (3), the rotating head (6) is in transmission connection with the consumables box (4), and a plurality of containers (8) for containing dyeing consumables are provided in the consumables box (4); a consumable material pressing head (5), the consumable material pressing head (5) corresponding to the tissue on the glass slide (7) above and below, and used to press the container (8) to quantitatively supply the staining consumable material to the tissue; A working platform (38) is provided on the protective shell (1), a positioning groove (14) is provided on the working platform (38), the positioning platform (3) is rotatably connected in the positioning groove (14), the positioning groove (14) is adapted to the consumable box (4), and during installation, the consumable box (4) slides into the inner wall of the positioning groove (14) and is positioned on the positioning platform (3); A guide block (15) is provided in the positioning groove (14), and a plurality of limiting grooves (16) are provided on the guide block (15), and the limiting grooves (16) are provided correspondingly to the through grooves (17) provided through the positioning platform (3); the outlet of the container (8) extends out of the consumable box (4) and the limiting grooves (16) and the through grooves (17) are provided correspondingly; The consumables box (4) is provided with a plurality of filling slots (18) for filling the container (8), and the bottom end of the container (8) is provided with a liquid outlet (19), and the liquid outlet (19) extends out of the filling slots (18); The top of the consumable box (4) is provided with a clearance groove (21) and a fixing card (22); the clearance groove (21) is provided on the side wall of the consumable box (4) and is provided corresponding to the rotating head (6); the fixing card (22) is open to one side of the clearance groove (21) and is fixed to the top of the consumable box (4); when the consumable box (4) is installed, the rotating head (6) passes through the clearance groove (21) and is clamped in the fixing card (22).

2. The miniature dyeing device according to claim 1, characterized in that: A ventilation module (9) is provided in the protective shell (1), and the ventilation module (9) is provided below the pull-out plate (2) and is used to blow the consumable liquid on the glass slide (7) off the glass slide (7), discharge it along a drainage channel (10) provided in the protective shell (1), and store it in a waste water box (11) in the protective shell (1).

3. The miniature dyeing device according to claim 1, characterized in that: A pressing motor (24) is provided in the protective housing (1), the output shaft of the pressing motor (24) is connected to a driving gear (25), the driving gear (25) is meshed with a longitudinally arranged driven rod (26), and the consumable pressing head (5) is fixedly mounted on the bottom end of the driven rod (26) to drive the consumable pressing head (5) to squeeze the container (8).

4. The miniature dyeing device according to claim 3, characterized in that: A guide cylinder (27) is provided in the protective housing (1) and is arranged corresponding to the driven rod (26). The driven rod (26) is slidably connected in the guide cylinder (27). A longitudinally arranged driven rack (28) is provided on the driven rod (26). The driven rack (28) is exposed from an exposed groove on the side wall of the guide cylinder (27). The driving gear (25) extends into the exposed groove and meshes with the driven rack (28) for transmission.

5. The miniature dyeing device according to claim 1, characterized in that: A rotating shaft (29) is provided on the protective housing (1), the top end of the rotating shaft (29) is transmission-connected to a rotating motor (30) fixedly installed in the protective housing (1), and the bottom end of the rotating shaft (29) is fixedly connected to the consumable pressing head (5).

6. The miniature dyeing device according to claim 5, characterized in that: A lifting bearing (31) is provided in the protective shell (1), the outer ring of the lifting bearing (31) slides longitudinally with the protective shell (1), and the rotating shaft (29) passes through the lifting bearing (31) and is fixedly connected to the inner ring of the lifting bearing (31).

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