Water bath heating device for antigen repair

Abstract

The invention discloses a water bath heating device for antigen repair, and relates to the technical field of antigen repair. The device comprises a heating box, a plurality of heating grooves are formed in the heating box, supporting plates are fixedly connected to the interiors of the heating grooves, moving rods are slidably connected to the side walls of the supporting plates, and elastic parts are arranged between the moving rods and the supporting plates; the upper end of the moving rod is fixedly connected with a clamping plate, the lower end of the moving rod is rotationally connected with a deflection rod, the middle of the deflection rod is rotationally connected with a supporting rod, and the supporting rod is fixedly connected to the supporting plate. Through mutual cooperation of a second structure of the support, the base, the flow guide plate and the limiting wheel, a slide can be clamped and limited during use, and large friction between the slide and the limiting wheel can be avoided through the rolling effect of the limiting wheel; therefore, the situation that cells on the surface of the slide fall off due to mechanical vibration generated between the slide and the base in the boiling process of the repair liquid is conveniently prevented.

Description

Technical Field

[0001] The invention relates to the technical field of antigen repair, in particular to a water bath heating device for antigen repair. Background Art

[0002] Immunohistochemical staining technology has become an indispensable detection method in modern pathological diagnosis. It is also used to determine the origin and classification of tumor tissues. Immunohistochemical technology is widely used in clinical pathological diagnosis, scientific experimental research and other related disciplines due to its advantages such as simple operation, high sensitivity and strong specificity. It has become one of the indispensable and important means. Immunohistochemistry mainly includes sample fixation, sectioning, dewaxing to water, inactivation, antigen repair, blocking, primary antibody incubation, secondary antibody incubation, color development, counterstaining and sealing. There are many processes and the time is long. Since formaldehyde is mostly used for specimen fixation in the early stage, aldehyde bonds are formed between amino acid residue molecules, and the antigenic determinants are masked or destroyed, resulting in the blocking of antigenic determinants, which hinders some antigen-antibody reactions. Whether the antigenic determinants are fully exposed is an important link in the quality of the staining effect. Antigen repair technology is a simple and effective enhancement method in immunohistochemical staining of paraffin sections. High temperature and high pressure repair and boiling repair are currently the two most commonly used repair methods.

[0003] The existing method generally involves placing the slide to be repaired on a slide rack, placing the slide rack in a container containing repair liquid and heating it in a microwave oven to boil. However, there is a large gap between the rack and the slide. After the liquid boils, the bubbles generated impact the slide, causing collisions and vibrations, which increases the risk of cells on the surface of the slide falling off the slide. For this reason, we propose a water bath heating device for antigen repair. Summary of the invention

[0004] The purpose of the present invention is to provide a water bath heating device for antigen repair, which can prevent the slide from vibrating and causing cells to fall off during the repair process.

[0005] To achieve the above object, the present invention provides the following technical solution: a water bath heating device for antigen repair, comprising a heating box, a plurality of heating slots are provided inside the heating box, a support plate is fixedly connected inside the heating slot, a moving rod is slidably connected to the side wall of the support plate, and an elastic member is provided between the moving rod and the support plate;

[0006] The upper end of the moving rod is fixedly connected to a clamping plate, and the lower end of the moving rod is rotatably connected to a deflection rod. The middle part of the deflection rod is rotatably connected to a support rod, and the support rod is fixedly connected to the support plate;

[0007] The free end of the deflection rod is rotatably connected to a base, a bracket is fixedly connected to the base, a plurality of guide plates are installed on both sides of the bracket, a guide groove is opened on the side wall of the guide plate, and limiting wheels are installed on both sides of the guide plate;

[0008] A lifting rod is fixedly connected to the bottom of the base, a threaded rod is connected to the internal thread of the lifting rod, a rotating shaft is fixedly connected to the bottom of the threaded rod, a driven wheel is fixedly connected to the outer surface of the rotating shaft, a driving wheel is meshedly connected to one side of the driven wheel, a transmission rod is fixedly connected to the side wall of the driving wheel, the other end of the transmission rod is arranged through the heating box, and a knob is fixedly connected to the other end of the transmission rod.

[0009] The use effect and principle of the present invention:

[0010] When antigen repair is required, first place the glass slide carrying the cell tissue in the positioning groove between the limiting wheels. At this time, the limiting wheels will clamp and fix the two sides of the glass slide to prevent mechanical vibration between the glass slide and the base during the boiling of the repair solution, which will cause the cells on the surface of the glass slide to fall off. When the glass slide is placed, turn the knob to drive the transmission rod to rotate in the opposite direction, which can drive the active wheel to rotate. When the active wheel rotates, it will drive the driven wheel to rotate, thereby driving the shaft to rotate, which can drive the threaded rod to rotate accordingly. When the threaded rod rotates, since the top of the lifting rod is fixed to the base, relative rotation will occur between the threaded hole and the threaded rod, so that the lifting rod can move up and down, thereby driving the base to rise or fall. When the base moves downward, it will drive the deflection rod to deflect around the support rod. At this time, the bottom end of the deflection rod will deflect downward, and the top end of the deflection rod will deflect upward, so that the top of the deflection rod will deflect downward. The end drives the moving rod to move, thereby driving the clamping plate to move toward the center of the heating tank, which is convenient for clamping the glass slide, avoiding mechanical vibration between the glass slide and the bracket, and further preventing cells from falling off; when the heating is completed, the slide needs to be cooled. At this time, the knob is turned to drive the transmission rod to rotate forward. At this time, the active wheel and the driven wheel will drive the rotating shaft and the threaded rod to rotate accordingly, thereby driving the lifting rod and the base to move upward, so that the glass slide moves upward. At this time, the clamping plates on both sides will automatically disengage from the glass slide to unlock, and the glass slide is also separated from the boiling repair liquid. At this time, the high-temperature glass slide can be cooled by external air cooling equipment, and when the airflow blows to the glass slide from the side, the airflow will be diverted by the guide plate, and then the airflow will flow along the guide groove to blow to both sides of the glass slide, thereby improving the cooling efficiency. When cooling is completed, the glass slide can be directly removed from the bracket.

[0011] Furthermore, the number of the heating tanks is set to four, and electric heating tubes are installed at the bottom of the four heating tanks.

[0012] Furthermore, a controller is installed on the side wall of the heating box, and the electric heating tube is electrically connected to the controller.

[0013] Furthermore, a through hole adapted to the moving rod is provided on the side wall of the support plate, and the elastic member is sleeved on the moving rod.

[0014] Furthermore, a protective pad is provided on the side wall of the clamping plate.

[0015] Furthermore, there are multiple guide plates, and positioning grooves are formed between the limiting wheels provided on adjacent guide plates.

[0016] Furthermore, the lifting rod is located at the center of the base, and a threaded hole matching the threaded rod is provided inside the lifting rod.

[0017] Furthermore, the bottom of the rotating shaft is rotatably connected to the bottom of the heating tank through a bearing.

[0018] Furthermore, the driving wheel and the driven wheel are both configured as bevel gears.

[0019] Furthermore, a mechanical seal is provided at the portion where the transmission rod is connected to the heating box.

[0020] The present invention has at least the following beneficial effects:

[0021] 1. The present invention can clamp and limit the glass slide during use by cooperating with the second structure of the bracket, base, guide plate and limiting wheel, and can also avoid large friction between the glass slide and the limiting wheel by utilizing the rolling effect of the limiting wheel, thereby preventing mechanical vibration between the glass slide and the base during the boiling of the repair liquid, which may cause the cells on the surface of the glass slide to fall off.

[0022] 2. The present invention cooperates with the active wheel, driven wheel, threaded rod, movable rod and other structures, so that when the base is lowered, the clamping plates on both sides can move toward the middle at the same time, clamp the glass slide from both sides, and further enhance the stability of the glass slide. When the base is raised, the glass slide can be separated from the repair liquid, which is not only convenient for draining, but also convenient for taking the glass slide.

[0023] 3. The present invention provides guide plates and guide grooves, which can guide the airflow when the glass slide is cooled by external airflow, so that the airflow is evenly blown to both sides of the glass slide, thereby improving the cooling efficiency.

[0024] 4. The present invention is overall provided with four heating tanks, and the electric heating tubes in individual heating tanks can be started or shut down respectively, so as to facilitate adaptive adjustment according to the number of glass slides to be heated.

[0025] Of course, any product implementing the present invention does not necessarily need to achieve all of the advantages described above at the same time. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 It is a three-dimensional schematic diagram of the overall structure of the present invention;

[0027] Figure 2 It is a three-dimensional schematic diagram of the support plate structure of the present invention;

[0028] Figure 3 It is a three-dimensional schematic diagram of the mobile rod structure of the present invention;

[0029] Figure 4 It is a three-dimensional schematic diagram of the transmission rod structure of the present invention;

[0030] Figure 5 For the present invention Figure 4 A magnified view of the structure at center A;

[0031] Figure 6 It is a three-dimensional schematic diagram of the guide plate structure of the present invention;

[0032] Figure 7 This is a schematic diagram of the air flow direction when the guide plate of the present invention is in use.

[0033] Reference numerals:

[0034] 1. Heating box; 2. Heating trough; 3. Support plate; 4. Moving rod; 5. Elastic member; 6. Clamp; 7. Deflection rod; 8. Support rod; 9. Base; 10. Bracket; 11. Guide plate; 12. Guide trough; 13. Limiting wheel; 14. Lifting rod; 15. Threaded rod; 16. Rotating shaft; 17. Driven wheel; 18. Driving wheel; 19. Transmission rod; 20. Knob; 21. Positioning slot; 22. Controller; 23. Cover plate. DETAILED DESCRIPTION

[0035] The following will be combined with the drawings in the embodiments of the present disclosure to clearly and completely describe the technical solutions in the embodiments of the present disclosure. Obviously, the described embodiments are only part of the embodiments of the present disclosure, rather than all the embodiments. Based on the embodiments in the present disclosure, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present disclosure.

[0036] See also Figure 1-7 The present invention provides a technical solution: a water bath heating device for antigen repair, comprising a heating box 1, a plurality of heating slots 2 are provided inside the heating box 1, a support plate 3 is fixedly connected inside the heating slot 2, a moving rod 4 is slidably connected to the side wall of the support plate 3, and an elastic member 5 is provided between the moving rod 4 and the support plate 3;

[0037] The upper end of the moving rod 4 is fixedly connected to a clamping plate 6, and the lower end thereof is rotatably connected to a deflection rod 7, and the middle part of the deflection rod 7 is rotatably connected to a support rod 8, and the support rod 8 is fixedly connected to the support plate 3;

[0038] The free end of the deflection rod 7 is rotatably connected to a base 9, a bracket 10 is fixedly connected to the base 9, a plurality of guide plates 11 are installed on both sides of the bracket 10, a guide groove 12 is opened on the side wall of the guide plate 11, and a limiting wheel 13 is installed on both sides of the guide plate 11;

[0039] A lifting rod 14 is fixedly connected to the bottom of the base 9, a threaded rod 15 is connected to the internal thread of the lifting rod 14, a rotating shaft 16 is fixedly connected to the bottom of the threaded rod 15, a driven wheel 17 is fixedly connected to the outer surface of the rotating shaft 16, a driving wheel 18 is meshedly connected to one side of the driven wheel 17, a transmission rod 19 is fixedly connected to the side wall of the driving wheel 18, the other end of the transmission rod 19 is arranged through the heating box 1, and the other end of the transmission rod 19 is fixedly connected to a knob 20.

[0040] It should be noted that a cover plate 23 is rotatably connected to one side of the heating box 1 via a hinge, so as to facilitate closing the heating box 1 .

[0041] According to the technical solution of this embodiment, the number of heating tanks 2 is set to four, and electric heating tubes are installed at the bottom of the four heating tanks 2, and a controller 22 is installed on the side wall of the heating box 1. The electric heating tubes are electrically connected to the controller 22. The set electric heating tubes are used to heat the repair fluid in the heating tank 2 separately. The set controller 22 can start or shut down a single electric heating tube separately, which is convenient for heating operations according to actual needs.

[0042] According to the technical solution of this embodiment, a through hole compatible with the moving rod 4 is opened on the side wall of the support plate 3, and the elastic member 5 is sleeved on the moving rod 4. The set elastic member 5 is used to provide elastic potential energy for the moving rod 4 to drive it to reset. When the support plate 3 is fixed in the heating tank 2, a gap is formed between the support plate 3 and the side wall of the heating tank 2, and the moving rod 4 moves in the gap space.

[0043] According to the technical solution of this embodiment, the shape of the splint 6 is a rectangular parallelepiped, and a protective pad is provided on the side wall of the splint 6. There are two splints 6. The two splints 6 can squeeze and fix the glass slide from both sides, and the protective pad can prevent the splint 6 from causing damage to the glass slide.

[0044] According to the technical solution of this embodiment, there are multiple guide plates 11, and positioning grooves 21 are formed between the limiting wheels 13 arranged on adjacent guide plates 11. By placing a glass slide into the positioning groove 21, the limiting wheels 13 can be used to limit and fix the two sides of the glass slide, and the rolling effect of the limiting wheels 13 can also avoid large friction between the glass slide and the limiting wheels 13. It should be noted that the part where the limiting wheel 13 contacts the glass slide does not carry any cell tissue.

[0045] According to the technical solution of this embodiment, the lifting rod 14 is located at the center of the base 9, and a threaded hole compatible with the threaded rod 15 is opened inside the lifting rod 14. When the threaded rod 15 rotates, since the top end of the lifting rod 14 is fixed to the base 9, relative rotation will occur between the threaded hole and the threaded rod 15, so that the lifting rod 14 can move up and down, thereby driving the base 9 to rise or fall. When the base 9 moves downward, it will drive the deflection rod 7 to deflect around the support rod 8. At this time, the bottom end of the deflection rod 7 will deflect downward, and the top end of the deflection rod 7 will deflect upward, so that the top end of the deflection rod 7 drives the moving rod 4 to move, thereby driving the clamping plate 6 to move toward the center of the heating tank 2, which is convenient for clamping the glass slide.

[0046] According to the technical solution of this embodiment, the bottom of the rotating shaft 16 is rotatably connected to the bottom of the heating tank 2 through a bearing. The driving wheel 18 and the driven wheel 17 are both configured as bevel gears. When the driving wheel 18 rotates, it drives the driven wheel 17 to rotate, thereby driving the rotating shaft 16 to rotate. When the rotating shaft 16 rotates, it can drive the threaded rod 15 to rotate.

[0047] It should be noted that the portion where the transmission rod 19 is connected to the heating box 1 is provided with a mechanical seal, so as to enhance the sealing performance between the transmission rod 19 and the heating box 1 when the transmission rod 19 rotates, thereby preventing leakage of the repair fluid.

[0048] The use principle and process of the present invention are as follows: when antigen repair is required, first place the glass slide carrying the cell tissue in the positioning groove 21 between the limiting wheels 13. At this time, the limiting wheels 13 will clamp and fix the two sides of the glass slide to prevent mechanical vibration between the glass slide and the base 9 during the boiling of the repair liquid, which will cause the cells on the surface of the glass slide to fall off. When the glass slide is placed, turn the knob 20 to drive the transmission rod 19 to rotate in the opposite direction, which can drive the active wheel 18 to rotate. When the active wheel 18 rotates, it will drive the driven wheel 17 to rotate, thereby driving the rotating shaft 16 to rotate, which can drive the threaded rod 15 to rotate accordingly. When the threaded rod 15 rotates, since the top end of the lifting rod 14 is fixed to the base 9, relative rotation will occur between the threaded hole and the threaded rod 15, so that the lifting rod 14 can move up and down, thereby driving the base 9 to rise or fall. When the base 9 moves downward, it will drive the deflection rod 7 to deflect around the support rod 8. At this time, the bottom end of the deflection rod 7 will deflect downward, and the top end of the deflection rod 7 will deflect upward. Rotate, so that the top of the deflection rod 7 drives the moving rod 4 to move, thereby driving the clamping plate 6 to move toward the center of the heating tank 2, so as to clamp the glass slide, avoid mechanical vibration between the glass slide and the bracket 10, and further prevent the cells from falling off; when the heating is completed, the glass slide needs to be cooled. At this time, the knob 20 is turned to drive the transmission rod 19 to rotate forward. At this time, the active wheel 18 and the driven wheel 17 will drive the rotating shaft 16 and the threaded rod 15 to rotate accordingly, thereby driving the lifting rod 14 and the base 9 to move upward, so that the glass slide moves upward. At this time, the clamping plates 6 on both sides will automatically disengage from the glass slide to unlock, and the glass slide and the boiling repair liquid are also separated. At this time, the high-temperature glass slide can be cooled by an external air cooling device, and when the airflow blows to the glass slide from the side, the airflow will be diverted by the guide plate 11, and then the airflow will flow along the guide groove 12 to blow to both sides of the glass slide, thereby improving the cooling efficiency. When the cooling is completed, the glass slide can be directly removed from the bracket 10.

[0049] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0050] For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to the specific circumstances. When an element is referred to as being "assembled on", "installed on", "fixed on" or "set on" another element, it can be directly on the other element or there can also be a centered element. When an element is considered to be "connected" to another element, it can be directly connected to the other element or there may be a centered element at the same time. The terms "vertical", "horizontal", "up", "down", "left", "right" and similar expressions used herein are only for illustrative purposes and are not intended to be the only implementation method.

[0051] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

[0052] In the description of this specification, the description with reference to the terms "one embodiment", "example", "specific example", etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present disclosure. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

Claims

1. An antigen repair water bath heating device, comprising a heating box (1), characterized in that: The heating box (1) is provided with a plurality of heating slots (2) inside, a support plate (3) is fixedly connected inside the heating slots (2), a moving rod (4) is slidably connected to the side wall of the support plate (3), and an elastic member (5) is provided between the moving rod (4) and the support plate (3); The upper end of the moving rod (4) is fixedly connected to a clamping plate (6), and the lower end thereof is rotatably connected to a deflection rod (7). The middle part of the deflection rod (7) is rotatably connected to a support rod (8), and the support rod (8) is fixedly connected to the support plate (3); The free end of the deflection rod (7) is rotatably connected to a base (9), a bracket (10) is fixedly connected to the base (9), a plurality of guide plates (11) are installed on both sides of the bracket (10), a guide groove (12) is provided on the side wall of the guide plate (11), and limiting wheels (13) are installed on both sides of the guide plate (11); The bottom of the base (9) is fixedly connected to a lifting rod (14), the internal thread of the lifting rod (14) is connected to a threaded rod (15), the bottom of the threaded rod (15) is fixedly connected to a rotating shaft (16), the outer surface of the rotating shaft (16) is fixedly connected to a driven wheel (17), one side of the driven wheel (17) is meshingly connected to a driving wheel (18), a transmission rod (19) is fixedly connected to the side wall of the driving wheel (18), the other end of the transmission rod (19) is arranged through the heating box (1), and the other end of the transmission rod (19) is fixedly connected to a knob (20).

2. The water bath heating device for antigen retrieval according to claim 1, characterized in that: The number of the heating tanks (2) is set to four, and electric heating pipes are installed at the bottom of the four heating tanks (2).

3. The water bath heating device for antigen retrieval according to claim 2, characterized in that: A controller (22) is installed on the side wall of the heating box (1), and the electric heating tube is electrically connected to the controller (22).

4. The water bath heating device for antigen retrieval according to claim 2, characterized in that: A through hole matched with the moving rod (4) is provided on the side wall of the support plate (3), and the elastic member (5) is sleeved on the moving rod (4).

5. The water bath heating device for antigen retrieval according to claim 4, characterized in that: A protective pad is arranged on the side wall of the clamping plate (6).

6. The water bath heating device for antigen retrieval according to claim 5, characterized in that: There are a plurality of guide plates (11), and positioning grooves (21) are formed between the limiting wheels (13) provided on adjacent guide plates (11).

7. The water bath heating device for antigen retrieval according to claim 6, characterized in that: The lifting rod (14) is located at the center of the base (9), and a threaded hole matching the threaded rod (15) is provided inside the lifting rod (14).

8. The water bath heating device for antigen retrieval according to claim 6, characterized in that: The bottom of the rotating shaft (16) is rotatably connected to the bottom of the heating tank (2) via a bearing.

9. The water bath heating device for antigen retrieval according to claim 8, characterized in that: The driving wheel (18) and the driven wheel (17) are both configured as bevel gears.

10. The water bath heating device for antigen retrieval according to claim 9, characterized in that: The portion where the transmission rod (19) is connected to the heating box (1) is provided with a mechanical seal.

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