Paeonia lactiflora 'Salaa' cut flower preservation method

Abstract

The invention discloses a Chinese herbaceous peony 'Salaa' cut flower fresh-keeping method. The Chinese herbaceous peony 'Salaa' cut flower fresh-keeping method comprises the step of inserting Chinese herbaceous peony 'Salaa' cut flowers into fresh-keeping liquid containing metformin hydrochloride and acarbose. The metformin hydrochloride and the acarbose are especially added into the Chinese herbaceous peony cut flower fresh-keeping liquid, so that the effect of synergistically adjusting flowering genes of Chinese herbaceous peony can be achieved, the flowering phase of Chinese herbaceous peony, especially 'Saxa' fresh cut flowers, is practically and effectively prolonged, and the flower diameter, the flower height, the fresh weight, the flower fragrance and the like are improved.

Description

Technical Field

[0001] The invention generally relates to a method for preserving cut peony flowers. Background Art

[0002] Fresh cut flowers refer to plant materials such as branches and flowers cut from living plants. Different cut flowers' own respiratory physiology, water physiology, material metabolism, oxidation balance, endogenous hormone levels and other factors will lead to aging of cut flowers to varying degrees. The life span of cut flowers in vases is limited after harvesting, and the post-harvest preservation technology cannot be guaranteed, resulting in the slow development of the scale of the traditional fresh cut flower market in my country. The physiological activities of cut flowers are still going on after they leave the mother plant, and the flowering period of cut flowers is shorter than that on the mother plant, so the viewing period of cut flowers is extremely short.

[0003] CN114903034A discloses that fresh-keeping liquid can promote the opening and delay the aging of various fresh cut flowers; CN112369411A discloses that fresh cut flowers can be treated with fresh-keeping agent to extend the flowering period, reduce water loss, withering and discoloration of fresh cut flowers, and solve the problems of short fresh-keeping period and poor fresh-keeping effect of fresh cut flowers; CN118140914A discloses that the preservative peony cut flower fresh-keeping liquid is designed with a preservative, and spermidine and glycine are added to play a synergistic role, so as to effectively extend the flowering period of the peony and / or improve the flower diameter; CN104255714B, CN102687716A, CN102365935B, etc. disclose that peony cut flowers are directly placed in various vase fresh-keeping liquids to extend the flowering period.

[0004] However, in view of the different specific requirements of peony cut flowers, there is still a need to further develop corresponding cut flower preservative solutions. Summary of the invention

[0005] The object of the present invention is to provide a fresh-keeping liquid for cut flowers of peony varieties, which can at least effectively prolong the flowering period of peony, especially 'Sala' cut flowers.

[0006] According to a first aspect of the present invention, a peony cut flower vase preservation liquid is provided, comprising:

[0007] Metformin hydrochloride 500-700 μmol / L; and acarbose 3-7 μmol / L.

[0008] The peony cut flower vase preservation liquid according to the present invention preferably further comprises:

[0009] NaCl 7~9g / L; KCl 0.1~0.3g / L; Na2HPO4.12H2O 3~4g / L;

[0010] KH2PO4 0.2~0.3g / L; citric acid 180~220mg / L; 8-hydroxyquinoline 90~110mg / L; salicylic acid 20~30mg / L; sucrose 15~25g / L.

[0011] The pH value of the peony cut flower vase preservation liquid according to the present invention can be 6.5-7.0, preferably about 6.8.

[0012] According to the peony cut flower vase preservation liquid of the present invention, 8-hydroxyquinoline, salicylic acid and citric acid are preferably first formed into an ethanol solution.

[0013] According to another aspect of the present invention, there is also provided a method for preparing the above-mentioned peony cut flower vase preservation liquid, comprising:

[0014] First, 8-hydroxyquinoline, salicylic acid and citric acid are formed into an ethanol solution;

[0015] Then, metformin hydrochloride, acarbose, NaCl, KCl, Na2HPO4.12H2O, and KH2PO4 are formed into an aqueous solution;

[0016] Then the ethanol solution was added to the aqueous solution and mixed thoroughly, and finally sucrose was added.

[0017] The preparation method according to the present invention also includes adjusting the pH value of the peony cut flower vase preservation solution to 6.5-7.0, such as about 6.8, with sodium hydroxide.

[0018] According to another aspect of the present invention, a method for preserving cut peony flowers is provided, comprising: inserting the cut peony 'Sala' flowers into the above-mentioned preservative solution. The preservative solution can be placed in a corresponding bottle.

[0019] The present invention adds metformin hydrochloride and acarbose to the peony cut flower preservative solution, thereby playing a role in synergistically regulating the flowering gene of peony, effectively extending the flowering period of peony, especially 'Sala' fresh cut flowers, and improving the flower diameter, flower height, fresh weight and flower fragrance, etc. In addition, the preservative solution components of the present invention are easy to obtain and low in cost, and are particularly suitable for industrial applications. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 Photos of the flowering changes of cut flowers of Paeonia lactiflora 'Sala' that were treated differently.

[0021] Figure 2 This is a bar chart showing the changes in flower height of Paeonia lactiflora 'Sala' after different treatments.

[0022] Figure 3 This is a bar chart showing the changes in flower diameter of Paeonia lactiflora 'Sala' after different treatments.

[0023] Figure 4 This is a bar chart showing the fresh weight changes of Paeonia lactiflora 'Sala' after different treatments. DETAILED DESCRIPTION

[0024] The present invention is described in detail below in conjunction with the accompanying drawings. It should be understood by those skilled in the art that the embodiments described below are only exemplary descriptions of the present invention and are not intended to limit the present invention in any way.

[0025] Example: Preparation of fresh-keeping liquid A

[0026] First, add 100 mg of 8-hydroxyquinoline ("Tianjin Komiou"), 200 mg of citric acid and 25 mg of salicylic acid into a 100 ml small glass beaker, then add 30 ml of anhydrous ethanol and stir to dissolve to obtain an ethanol solution.

[0027] Then, in a 1L large glass beaker, add: NaCl 8g, KCl 0.2g, Na2HPO4.12H2O 3.63g, KH2PO4 0.24g, 600μmol metformin hydrochloride and 5μmol acarbose, and finally add 970mL distilled water and mix well to form an aqueous solution.

[0028] Pour the ethanol solution in the small glass beaker into the large glass beaker, mix it thoroughly with the aqueous solution therein, then add 20 g of sucrose, stir and mix evenly, add sodium hydroxide to adjust the pH value of the mixed solution to 6.8 to obtain the standby preservative solution A.

[0029] Comparative Example 1: Preparation of preservative liquid CK2

[0030] The same procedures as in Example 1 were followed except that the amount of metformin hydrochloride and acarbose added was changed to 0, thereby obtaining a fresh-keeping solution CK2 for standby use.

[0031] Comparative Example 2

[0032] The laboratory water maker produces pure water, marked as CK1.

[0033] Collecting and processing plant samples

[0034] The cut flower material on May 17, 2023 is 'Sala'. Pick peony branches that are in the opening stage (level II) of flowering, select cut flowers with the same growth state, cut them at a 45° angle in water to a length of about 20-25cm, and retain two compound leaves. 150 branches are needed, and 5 replicates are set for each treatment. 5 of them are used to observe morphological characteristics and measure flower diameters at the same time every day. The indoor temperature is controlled at 20-22℃ and the relative humidity is 50%-60%. They are inserted into 200ml of distilled water or bottle inserts respectively.

[0035] Fresh weight of flower branches: Starting from the day of vase placement, measure the weight of the flower branches at the same time every day.

[0036] Flower diameter (flower diameter): Starting from the day of vase placement, use the cross method to measure the flower diameter at the same time every day.

[0037] Vase life: the number of days from 0 hours after vase insertion to when 50% of the petals begin to wilt and fall off. The vase life ends when the flower buds develop to the initial wilting stage, i.e. the edges of the petals begin to curl inward and wilt, losing their expansion.

[0038] Experiment on the treatment of cut flowers in vases

[0039] The opening process of cut flowers of Peony 'Sala' can be divided into seven stages: coloring stage (I), opening stage (II), initial opening stage (III), half-opening stage (IV), full opening stage (V), beginning of decay stage (VI), and decay stage (VII). The flowers should be placed in a vase from the opening stage until the decay stage.

[0040] The above-mentioned preservative solution, control base solution and 200 ml of pure water were respectively contained in long-necked vases (300 ml), and the above-mentioned cut flowers were respectively inserted into open vases, and the phenotypic state of the cut flowers was observed for 7 consecutive days (2023.5.17-2023.5.24). Figure 1 Photos of the flowering changes of cut flowers of Paeonia lactiflora 'Sala' that were treated differently.

[0041] The 'Sala' peony cut flowers absorb water quickly and bloom during the vase placement process, and then the petals wilt and die. Figure 1 It can be seen that all three treatments reached full bloom after 24 hours, CK1, CK2, and A began to curl at 72 hours, but CK1 and CK2 had petal drop in the later period, but A did not drop petals. Under the A treatment, the petals opened earlier, but wilted earlier; it can make the petals bloom earlier, and at the same time improve the stiff flower phenomenon of cut flowers, and the flowering period is extended by about 1 day on average compared with the control; the A treatment has the most significant effect.

[0042] Figure 2 This is a bar chart showing the changes in flower height of Paeonia lactiflora 'Sala' after different treatments.

[0043] Figure 3 This is a bar chart showing the changes in flower diameter of Paeonia lactiflora 'Sala' after different treatments.

[0044] Figure 4 This is a bar chart showing the fresh weight changes of Paeonia lactiflora 'Sala' after different treatments.

[0045] like Figure 2As shown in the figure, the flower height did not change significantly in the early and late stages, but the difference increased during the gradual opening of the flowers, reaching the highest peak at the 60th hour, and generally showed a trend of first rising and then falling. The addition of different concentrations was significantly better than CK1 at the best viewing period (P<0.05), and from the 60th to 96th hour, it can be seen that the flower height effect of peony cut flowers treated with solution A was significantly higher than that of other treatment groups (P<0.05). This shows that treatment A effectively improves the quality of cut flowers.

[0046] like Figure 3 As shown in the figure, the overall change in the flower diameter of the vase-inserted peony cut flowers showed a trend of first rapid increase and then slow decrease. In the early stage of vase insertion, the flowers opened rapidly after the flower branches absorbed water quickly, and the flower diameter changed rapidly, reaching the highest at the 60th hour. From the 0th hour to the 120th hour, the peony cut flowers treated with the added solution were always larger than the CK1 flower diameter; as can be seen from the figure, the A solution treatment was generally larger than the CK1 and CK2 treatments before the 60th hour of vase insertion. After the 108th hour, the significant difference between the three treatments narrowed. Among them, the A treatment had the most obvious effect, and the flower diameter increased significantly during the viewing period (P<0.05).

[0047] like Figure 4 As shown in the figure, the quality of fresh samples of cut peony flowers in vase showed a trend of first increasing and then decreasing. The quality of fresh samples of cut flowers reached the maximum at 48 hours after vase insertion, and then the quality began to decline continuously. The quality of fresh samples under different treatments was always at a high level, which was significantly higher than CK1 (P<0.05), and the quality of fresh samples under treatment A was significantly higher than that of other concentrations (P<0.05). This shows that treatment A is conducive to maintaining the quality of fresh samples of cut peony flowers and can effectively improve the quality of cut flowers.

[0048] Table 1 shows the content of floral aroma substances in Paeonia lactiflora 'Sala' after different treatments.

[0049]

[0050] As shown in Table 1, these compounds include many different types of organic compounds, including aldehydes, alkanes, alcohols, and aromatic compounds.

[0051] Increase in nonanal (124-19-6) content: The nonanal content (5.5094) of treatment A was significantly higher than that of CK1 (1.9231) and CK2 (4.3462). This suggests that treatment A may have promoted the synthesis or release of nonanal, which may have improved the aroma characteristics of Paeonia lactiflora ‘Sala’ and enhanced its aroma intensity.

[0052] Increase in decanal (112-31-2) content: The decanal content of treatment A (6.9472) was significantly higher than that of CK1 (3.6538) and CK2 (4.1923), which may indicate that treatment A helps to increase the production of this compound, thereby enhancing the complexity and layering of the aroma.

[0053] Significant changes in the content of phenylethanol (60-12-8): The phenylethanol content of treatment A (521.8951) was close to that of CK1 (518.1731), but higher than that of CK2 (239), indicating that treatment A may have a more positive effect on the synthesis or release of phenylethanol. Phenylethanol is a compound with a floral odor. Increasing its content helps to enhance the floral characteristics of peony.

[0054] Increase in the content of n-octadecane (112-88-9): The n-octadecane content of treatment A (18.2330) was significantly higher than that of CK1 (5.7115) and CK2 (15.2692), indicating that treatment A may have an advantage in the synthesis of n-octadecane. n-Octadecane is a common alkane compound that usually has an oily feel and may contribute to the richness of the aroma.

[0055] Increase in the content of n-hexadecane (544-76-3): The n-hexadecane content of treatment A (24.8893) was higher than that of CK1 (17.6546) and CK2 (20.5192), which means that treatment A may help to generate more n-hexadecane, further enhancing the thickness and persistence of the aroma.

[0056] Improvement of overall aroma substances: The contents of overall aroma compounds (such as nonanal, decanal, phenylethanol, n-octadecane, n-hexadecane, etc.) in treatment A were generally higher than those in CK1 and CK2, which means that treatment A had a comprehensive effect on improving the aroma quality of Paeonia lactiflora ‘Sala’ and could optimize the level and richness of the aroma.

[0057] In summary, treatment A can significantly increase the content of multiple key aroma compounds, and compared with treatments CK1 and CK2, it not only helps to improve the concentration and complexity of the aroma, but also enhances the characteristics of the floral aroma, so it has obvious advantages in aroma improvement. It can be seen that the preservative solution of the present invention can not only effectively delay the aging process of Paeonia lactiflora 'Sala' peony cut flowers, but also has the effects of improving fresh weight, flower height, flower diameter, floral aroma, etc.

Claims

1. A peony cut flower vase preservation liquid, comprising: Metformin hydrochloride 500-700 μmol / L; and acarbose 3-7 μmol / L.

2. The peony cut flower vase preservation liquid according to claim 1, further comprising: NaCl 7~9g / L; KCl 0.1~0.3g / L; Na2HPO4.12H2O 3~4g / L; KH2PO4 0.2~0.3g / L; citric acid 180~220mg / L; 8-hydroxyquinoline 90~110mg / L; salicylic acid 20~30mg / L; sucrose 15~25g / L.

3. The peony cut flower vase preservation liquid according to claim 2, wherein the pH value is 6.5 to 7.

0.

4. The peony cut flower vase preservation liquid according to claim 2, wherein the 8-hydroxyquinoline, salicylic acid and citric acid are first formed into an ethanol solution.

5. A method for preparing the peony cut flower vase preservation liquid according to claim 2, comprising: First, 8-hydroxyquinoline, salicylic acid and citric acid are formed into an ethanol solution; Then, metformin hydrochloride, acarbose, NaCl, KCl, Na2HPO4.12H2O, and KH2PO4 are formed into an aqueous solution; Then the ethanol solution was added to the aqueous solution and mixed thoroughly, and finally sucrose was added.

6. The preparation method according to claim 5, further comprising adjusting the pH value of the peony cut flower vase preservation solution to 6.5-7.0 with sodium hydroxide.

7. A method for preserving cut peony flowers, comprising: Cut flowers of Paeonia lactiflora 'Sala' are inserted into the preservative solution according to any one of claims 1-4.

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