Application of cyclocitral to preparation of pest control agent

Abstract

The invention discloses application of cyclocitral to preparation of a pest control agent. The cyclocitral is one or a mixture of two or more of beta-cyclocitral, alpha-cyclocitral and isocyclocitral. According to the method, the parasitic wasp attractant is developed by exploring the active odor volatile matter capable of remarkably regulating and controlling the selectivity of the parasitic wasps, the parasitic wasp attractant is used for trapping natural enemy insects, namely the parasitic wasps, the density of the field parasitic wasps and the host searching efficiency of the parasitic wasps are improved, and therefore the purpose of efficiently controlling pests is achieved.

Description

Technical Field

[0001] The invention belongs to the technical field of biochemical pesticides, and particularly relates to application of cyclocitral in preparing parasitic wasp attractants and related attractants. Background Art

[0002] Using natural enemy insects for biological pest control is an important development direction of pest control at home and abroad. Parasitic wasps, as an important parasitic natural enemy insect, play an important role in the green control of crop pests. However, on the one hand, because the population density of parasitic wasps is limited in the natural state, it is difficult to achieve efficient pest control effects; on the other hand, although many parasitic wasps can be reproduced and produced in large quantities and used for field pest control. However, due to the openness and complexity of the natural environment in the field and the short flight distance of parasitic wasps, the parasitism rate and residence rate of artificially released parasitic wasps in the field are not high. Summary of the invention

[0003] The present invention determines the effects of a series of odor volatiles on the selection behavior of parasitic wasps through a "Y"-type olfactory selection system, and finds that parasitic wasps have a high selectivity for cyclocitral, and statistical analysis also shows that compared with the blank control, the selectivity of cyclocitral for parasitic wasps presents a significant difference (P value <0.05), which indicates that the volatiles can significantly affect the directional behavior of parasitic wasps.

[0004] Based on this, the present invention provides the use of cyclocitral for preparing a pest control agent for controlling lepidopteran pests, coleopteran pests, hymenopteran pests or dipteran pests, wherein the cyclocitral is a mixture of one or two or more of β-cyclocitral, α-cyclocitral and isocitral. The pest control is achieved by attracting natural enemy insect parasitic wasps. Accordingly, the present invention also provides the use of cyclocitral for preparing a parasitic wasp attractant.

[0005] The present invention also provides a related parasitic wasp attractant, wherein the active component of the attractant is cyclocitral, and the cyclocitral is a mixture of one or two or more of β-cyclocitral, α-cyclocitral and isocyclocitral.

[0006] Furthermore, the present invention determines the effect of different concentrations of cyclocitral on the selection behavior of parasitic wasps, and while further confirming that the volatile compound can significantly regulate the selection behavior of parasitic wasps, it also shows that the regulation is closely related to the concentration of its compound, but it is not a simple positive correlation, and its effect is significant within a certain concentration range.

[0007] In a preferred embodiment, the mass percentage of cyclocitral in the attractant or control agent of the present invention is 0.5% to 95%. In a preferred embodiment, the mass percentage of cyclocitral is 5% to 10%.

[0008] The present invention develops parasitic bee attractants by exploring active odor volatiles that can significantly regulate the selectivity of parasitic bees, and uses parasitic bee attractants to attract natural enemy insects - parasitic bees, thereby increasing the density of parasitic bees in the field and the efficiency of parasitic bees in finding hosts, thereby achieving the purpose of efficiently controlling pests. In addition, by using attractants, the parasitic rate of artificially released parasitic bees in the field and the pest control effect can also be enhanced.

[0009] Cyclocitral is an important class of volatile terpenoid compounds, which can be obtained by various methods such as plant extraction, chemical synthesis, and biosynthesis, including isocitral, α-cyclocitral, and β-cyclocitral. Among them, isocitral is mainly used in the formulation of floral fragrances, and can also be used for the fragrance of household cleaners; α-cyclocitral and are important intermediates for terpenoid compounds such as synthetic spices and carotenoids; β-cyclocitral is an important substance released by algae and causes odor in water bodies. Studies have also found that this compound is a widely effective plant root growth promoter, which can act on monocotyledonous and dicotyledonous plants at the same time and can be used to enhance the vitality of crops under stress environments. The present invention uses it as an active substance of an attractant, which has the advantages of low toxicity, easy degradation, and environmental friendliness. BRIEF DESCRIPTION OF THE DRAWINGS

[0010] Figure 1 The graph shows the tendency of Pleuronectinus pallidipes to 24 different odor volatiles in Example 1; the samples in the graph are attractants prepared with various compounds, and the control is an attractant without adding odor volatiles.

[0011] Figure 2 This is the effect of different concentrations of β-cyclocitral on the selection behavior of Pseudocypterus pallens in Example 2; the samples in the figure are attractants of different concentrations prepared with β-cyclocitral, and the control is: attractant without adding β-cyclocitral. DETAILED DESCRIPTION

[0012] Unless otherwise specified, the scientific and technical terms used herein are understood according to the knowledge of ordinary technicians in the relevant fields.

[0013] The present invention is further described below by way of examples. The examples are provided to illustrate the present invention in detail, but the present invention is not limited thereto.

[0014] Parasitic wasps belong to the order Hymenoptera, and can be used to control lepidopteran pests, coleopteran pests, hymenopteran pests and dipteran pests in crops. The types of parasitic wasps reported at present include: trichogrammatids (trichogrammatids of pine caterpillars, trichogrammatids of broad-eyed, trichogrammatids of corn borers, etc.), braconids (velvet braconids of stem borers, soft braconids of wheat moth, coccinella braconids, velvet braconids of hawk moths, etc.), ichneumonids (black-spotted ichneumonids, black-spotted ichneumonids of gypsy moths, hanging cocoon ichneumonids, etc.), etc. The following examples select the pale-footed side groove braconid as a research representative, but the present invention is not limited to such parasitic wasps.

[0015] The attractant or control agent of the present invention can be a common pesticide formulation such as powder, emulsifiable concentrate, suspension, dry suspension, concentrated emulsion, sustained release agent, nano preparation, water emulsion, spray, etc. Those skilled in the art can select suitable adjuvants according to the relevant formulations, such as common adjuvants are one or more of solvents, emulsifiers, solubilizers, etc. Preferably, the solvent can be one or more of organic solvents such as paraffin oil, lanolin, water, ethanol, methanol, acetone, cyclohexanone, petroleum ether, petroleum aromatics, pure benzene, ethyl acetate, toluene, xylene, etc. The emulsifier is Tween, ethylene glycol ester, span, propylene glycol ester and / or polyethylene glycol type nonionic surfactant, etc. Preferably, the solubilizer is one or more of polyethylene glycol, sodium dodecylbenzene sulfonate, polysorbate, potassium dodecylbenzene sulfonate, sorbitol polyoxyethylene ether, lignin sulfonate, etc.

[0016] The following examples use a "Y" type olfactometer system to test the directional behavior of P. pallidipes. In addition, the compounds, reagents, etc. used in the following examples are all commercially available products.

[0017] Example 1: The inventors found that the pale-footed Braconidae showed a clear tendency to β-cyclocitral.

[0018] The selection idea of ​​the substances used in this embodiment is: 24 different types of volatile compounds with odors released by host plants, pests and their feces: indole, n-nonanal, hexanal, n-hexane, heptadecane, β-cyclocitral, cis-2-pentenol, n-hexanol, 2-phenylethanol, benzyl alcohol, linalool, 1-penten-3-ol, isooctyl alcohol, geraniol, leaf acetate, leaf isovaleric acid, leaf caproate, α-pinene, β-pinene, ocimene, α-phellandrene, α-farnesene, 3-octanone and (R)-(+)-limonene.

[0019] Preparation of attractant: Weigh 1.0 g of each compound, add emulsifier TW-80 (1 mL), then dissolve and dilute to 100 mL with paraffin oil, mix well, and prepare an attractant with a volatile compound content of 0.1 g / mL (10%).

[0020] Determination scheme: The above 24 attractants were used to determine the selection behavior of the parasitic wasp Pleuronectin through the "Y"-type olfactory system. For the specific determination scheme, please refer to the relevant records in "Zhang Tongyao et al., 2024. Attraction effect of two host plants on Pleuronectin and analysis of volatile components. Chinese Journal of Applied Entomology [J], 2024, 6(1): 62-73."

[0021] The results of the test are as follows Figure 1 As shown by Figure 1 The results show that the parasitic wasp showed different degrees of selectivity for 24 different types of volatile odor compounds. Among them, the selectivity for β-cyclocitral was the highest, with a selectivity rate of 67.86%. The statistical analysis results also showed that compared with the blank control, the parasitic wasp's selectivity for β-cyclocitral showed a very significant difference (P<0.01), which indicates that β-cyclocitral can significantly affect the tendency behavior of the parasitic wasp and show an attractant effect.

[0022] Example 2: The inventors found that the attracting effect of β-cyclocitral on the pale-footed braconid wasp is concentration-dependent.

[0023] plan:

[0024] A certain amount of β-cyclocitral was weighed, dissolved in paraffin oil and prepared into attractants with mass concentrations of 0.005 (0.5%), 0.01 (1.0%), 0.05 (5.0%), 0.1 (10%), 0.5 (50%) and 0.95 g / mL (95%), respectively, and then the selection behavior of Pleuronectin wasp was determined using the "Y" type olfactory system described in Example 1.

[0025] The results are as follows Figure 2 As shown in the figure, different concentrations of β-cyclocitral have an effect on the selection behavior of P. pallidipes. In particular, in the mass concentration range of 5.0%-10%, the selection rate of P. pallidipes for β-cyclocitral is relatively high, all above 67%, among which the selection rate is the highest when the mass concentration is 5.0%, and its selection rate is as high as 86.67%, indicating that β-cyclocitral shows a highly efficient attractant effect on P. pallidipes within this concentration range.

Claims

1. The use of cyclocitral in preparing a lepidopteran pest, coleopteran pest, hymenopteran pest or dipteran pest control agent, wherein the cyclocitral is one or a mixture of two or more of β-cyclocitral, α-cyclocitral and isocyclocitral.

2. The use of cyclocitral for preparing a parasitic wasp attractant, wherein the cyclocitral is a mixture of one or two or more of β-cyclocitral, α-cyclocitral and isocitral.

3. The use according to claim 1 or 2, characterized in that: The mass percentage of cyclocitral in the attractant or control agent is 0.5% to 95%.

4. The use according to claim 1 or 2, characterized in that: The mass percentage of cyclocitral in the attractant or control agent is 5% to 10%.

5. A parasitic wasp attractant, characterized in that: The active component of the attractant is cyclocitral, and the cyclocitral is one or a mixture of two or more of β-cyclocitral, α-cyclocitral and isocyclocitral.

6. The parasitic wasp attractant according to claim 5, characterized in that The mass percentage of cyclocitral in the attractant is 0.5% to 95%.

7. The parasitic wasp attractant according to claim 5, characterized in that The mass percentage of cyclocitral in the attractant is 5% to 10%.

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