A fungicidal composition for controlling rust of fig

Abstract

The present application belongs to the technical field of fungicides, and particularly relates to a fungicidal composition for preventing and treating rust of figs. The fungicidal composition is used for preventing and treating rust of figs, and is composed of triforine and flusilazole or diclomezine. The present application compounding triforine and flusilazole or diclomezine in a certain mass ratio, and the compounding combination shows a synergistic effect on the rust pathogen of figs, Pucciniastrum ambrosiae, in a certain mass ratio range, and compared with single components, can improve the prevention and treatment effect on the rust of figs, delay the generation and development of the pathogen resistance, and reduce the resistance risk of the fungicidal composition. Secondly, the fungicidal composition can broaden the compounding combination of triforine, and provide support for fully developing the compounding medicament of triforine.

Description

Technical Field

[0001] This invention belongs to the field of fungicide technology, specifically relating to a fungicide composition for preventing and controlling fig rust disease. Background Technology

[0002] Fig rust is prone to occur in rainy years and in damp orchards within greenhouses. The disease initially infects the base of the leaves, gradually spreading upwards. Early symptoms include rust-colored veins. As the disease progresses, reddish-brown lesions appear on the upper surface of the leaves, which eventually enlarge and merge. Numerous rust-colored powdery spores are produced on the underside of diseased leaves. In orchards with severe rust outbreaks, shaking the tree or branches releases spores into the air, creating a smoke-like dispersion. Fig rust causes premature leaf drop and, when combined with twig dieback, negatively impacts yield and quality.

[0003] The pathogen causing fig rust is *Uredo sawadae Ito.*, which only exhibits a urediniospore generation, while the aerospore and teliospore generations are unknown. It belongs to the class of imperfect rust fungi. Urediniospores are borne on the underside of leaves and are brown; the urediniospores are yellow to yellowish-brown, with tuberculate spines and thick walls. In fig production, chemical control of fig rust is the primary method, using fungicides such as thiophanate-methyl, tebuconazole, difenoconazole, triadimefon, tebuconazole, and cyazofamid. However, pesticide resistance is a significant issue in chemical control. Long-term, excessive, and unscientific use of pesticides has led to varying degrees of resistance in the pathogen to many existing agents, resulting in a gradual decrease in control effectiveness.

[0004] Trifluoropyridamole is a succinate dehydrogenase inhibitor (SDHI) class of fungicides and nematicides. In controlling pathogens, trifluoropyridamole inhibits pathogen respiration by interfering with the tricarboxylic acid cycle in respiratory electron transport complex II, inhibiting mitochondrial function, preventing energy production, and thus inhibiting pathogen growth, ultimately leading to pathogen death. In controlling nematodes, it inhibits succinate dehydrogenase activity, disrupting ATP production in the mitochondria of plant pathogenic nematodes, and can be used to control various fungal diseases and nematodes. Summary of the Invention

[0005] The purpose of this invention is to provide a fungicide composition for the prevention and control of fig rust. In the process of chemical control of pests and diseases, drug resistance is an issue that cannot be ignored. With the long-term, large-scale, and unscientific use of pesticides, fig rust pathogens have developed varying degrees of resistance to many existing pesticides, resulting in a year-by-year decrease in the effectiveness of control.

[0006] To achieve the above objectives, the present invention provides the following technical solution:

[0007] A fungicide composition for controlling fig rust, said fungicide composition being a compound of trifluoropyridine amine and flusilazole or phenoxybenzanone.

[0008] Furthermore, the mass ratio of trifluoropyridine to fluorosilazole is 1-40:15-1.

[0009] Furthermore, the mass ratio of trifluoropyridine to phenoxyacetic acid is 1-20:20-1.

[0010] A bactericide comprising the bactericidal composition thereof, wherein the bactericidal composition comprises 0.75-55% by mass of the total mass of the bactericide.

[0011] Compared with the prior art, the present invention has the following beneficial effects:

[0012] (1) In this invention, trifluopyridine is compounded with flusilazole or phenoxyacetic acid in a certain mass ratio. The compound combination shows a synergistic effect on fig rust pathogen Urinaria figosae within a certain mass ratio range. Compared with single components, it can improve the control effect on fig rust, and at the same time delay the emergence and development of pathogen resistance, and reduce the risk of drug resistance of the fungicide composition.

[0013] (2) The bactericidal composition of the present invention can broaden the combination of trifluoropyridine amines and provide support for the full development of compound agents of trifluoropyridine amines. Detailed Implementation

[0014] The technical solution of this invention patent will be clearly and completely described below. Obviously, the described embodiments are only some, not all, of the embodiments of this invention. Based on the embodiments of this invention, all other embodiments obtained by those skilled in the art without inventive effort are within the scope of protection of this invention.

[0015] Example Indoor bioactivity test of trifluoropyridine combined with fig rust disease

[0016] 1. Test strain

[0017] Fresh urediniospores of *Urpinus rubra* were collected from fig leaves exhibiting typical symptoms.

[0018] 2. Test reagents

[0019] 86% trifluralinamide technical grade (Syngenta Crop Protection AG, Switzerland), 95% flusilazole technical grade (Shandong Weifang Runfeng Chemical Co., Ltd.), 98% phenoxyacetic acid technical grade (Hubei Hengjingrui Chemical Co., Ltd.)

[0020] The test reagent was first dissolved in dimethyl sulfoxide, and then diluted with 0.1% Tween-80 aqueous solution to prepare a single-agent stock solution. Multiple ratios were set up, and five mass concentration gradients were set for each single agent and ratio mixture according to the proportional method.

[0021] 3. Test methods (Refer to "NY / T 1156.1-2006 Guidelines for Indoor Bioassay of Pesticides - Fungicides Part 1: Inhibition of Pathogenic Fungal Spore Germination Test - Concave Slide Method")

[0022] Fresh urediniospores of *Ficus ficus* rust fungus were collected from fig leaves and resuspended in deionized water to a concentration of 1×10⁻⁶. 7 The concentration of the reagent was increased to 0.5% glucose solution and 0.5% glucose solution was added. 0.5 mL of the reagent and 0.5 mL of spore suspension were mixed thoroughly, and then the mixture was pipetted onto a concave glass slide using a micropipette. The slide was placed in a petri dish with a shallow layer of water, covered, and incubated at 25°C. Each treatment was repeated four times, with a 0.1% Tween-80 aqueous solution used as a blank control.

[0023] When the germination rate of the blank control spores reached over 90%, the germination status of spores in each treatment was examined. Three fields of view were randomly selected for each replicate of each treatment, and a total of 200 spores were collected. The number of germinating spores and the total number of spores were recorded. Germination was defined as a spore germ tube length greater than the short radius of the spore. Based on the results, the relative inhibition rate of spore germination for each treatment was calculated.

[0024]

[0025] 4. Data Analysis

[0026] Data statistical analysis was performed using DPS software. Linear regression was conducted with the logarithm of fungicide concentration as x and the corresponding relative spore germination inhibition rate probability as y to determine the EC50 toxicity of the fungicide against the target pathogen. 50 The value was calculated, and the cotoxicity coefficient (CTC) was calculated according to Sun Yunpei's method.

[0027] 5. Measurement Results

[0028] The synergistic effect of the agent was evaluated based on the calculated co-toxicity coefficient (CTC). CTC ≤ 80 indicates antagonistic effect, 80 < CTC < 120 indicates additive effect, and CTC ≥ 120 indicates synergistic effect. The results are shown in Table 1-2.

[0029] Table 1. Indoor bioactivity assay of trifluoropyridine and flusilazole combination against *Ustilago maydis* rust.

[0030] Drug Name and Proportion <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Trifluoropyridine 0.9357 100.0000 -- -- Fluorosilazole 12.7031 7.3659 -- -- Trifluoropyridine 1: Flusilazole 15 5.6311 16.6166 13.1555 126.3090 Trifluoropyridine 1: Flusilazole 10 3.0037 31.1516 15.7872 197.3218 Trifluoropyridine 1: Flusilazole 5 3.3702 27.7639 22.8049 121.7453 Trifluoropyridine 1: Flusilazole 1 1.0531 88.8520 53.6830 165.5124 Trifluoropyridine 5: Flusilazole 1 0.7413 126.2242 84.5610 149.2700 Trifluoropyridine 10: Flusilazole 1 0.5462 171.3109 91.5787 187.0641 Trifluoropyridine 20: Flusilazole 1 0.8075 115.8762 95.5889 121.2235 Trifluoropyridine 30: Flusilazole 1 0.6239 149.9760 97.0118 154.5956 Trifluoropyridine 40: Flusilazole 1 0.7774 120.3627 97.7406 123.1450

[0031] As shown in Table 1, the co-toxicity coefficients of trifluoropyridine and flusilazole against *Ustilago maydis* rust fungus were all greater than 120 within a mass ratio of 1-40:15-1, indicating a synergistic effect.

[0032] Table 2. Indoor bioactivity assays of the combination of trifluopyridine and phenoxybenzanol with *Ustilago maydis* rust.

[0033]

[0034]

[0035] As shown in Table 2, the co-toxicity coefficient of triflupyridine and phenoxyacetic acid in the compound of 1-20:20-1 against *Ustilago maydis* is greater than 120, indicating a synergistic effect.

[0036] In summary, this invention combines trifluopyridine with flusilazole or phenoxybenzanol in a certain mass ratio. Within a certain mass ratio range, the combination exhibits a synergistic effect against *Ustilago maydis*, the pathogen of fig rust. Compared with single components, it can improve the control effect on fig rust, while also delaying the emergence and development of drug resistance in the pathogen and reducing the risk of drug resistance in the fungicidal composition.

Claims

1. A bactericidal composition for controlling fig rust, characterized in that, The bactericidal composition is a compound of trifluoropyridine and flusilazole, wherein the mass ratio of trifluoropyridine to flusilazole is 1-40:15-1.

2. A bactericide, characterized in that, The bactericide includes the bactericide composition according to claim 1, wherein the mass of the bactericide composition accounts for 0.75-55% of the total mass of the bactericide.

Images