Preparation method of cholesterol-consuming nano-reactor and application thereof

The "one-pot method" to prepare ZIF@COD/IR820 nanoparticles and the membrane protein extraction method to prepare HM/ZIF@COD/IR820 nanoreactor solved the problems of complex synthesis and insufficient targeting of existing liver cancer treatment drugs, achieved significant tumor cell targeting and cholesterol consumption effects, and improved the treatment effect of liver cancer.

CN119499373BActive Publication Date: 2025-10-10NANJING QIHUA INTERDISCIPLINARY SCIENCE & TECHNOLOGY CO LTD
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Patent Information

Application Number
CN202411474957.X
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-10-22
Publication Date
2025-10-10
Estimated Expiration
2044-10-22

AI Technical Summary

Technical Problem

Among existing liver cancer treatment drugs, the synthesis steps of 2-methylimidazole zinc metal frame nanoreactor are complex, the cholesterol oxidase loading efficiency is low, and it lacks targeting, resulting in insignificant cholesterol consumption effect, affecting the treatment effect.

Method used

ZI F@COD/I R820 nanoparticles were prepared by a one-pot method. Hepatocellular carcinoma and erythrocyte membrane fragments were obtained by membrane protein extraction. The fragments were combined in a liposome extruder and loaded with COD/I R820 nanoparticles using hybrid cell membrane camouflage. Cholesterol oxidase solution was prepared in one pot, centrifuged, and the precipitate was washed to obtain a cholesterol-consuming nanoreactor named HM/ZI F@COD/I R820.

Benefits of technology

The prepared nanoreactor has excellent cholesterol-consuming nanoparticles with excellent targeting, which can increase the cholesterol consumption of tumor cells, promote tumor cell apoptosis, and improve the treatment effect of liver cancer.

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Abstract

The application provides a preparation method of a cholesterol-consuming nano reactor and application thereof, and belongs to the technical field of biological medicines. The preparation method comprises the following steps: adding a zinc acetate solution dropwise into a 2-methylimidazole solution to obtain a mixed solution; adding a cholesterol oxidase solution and a new indocyanine green solution dropwise into the mixed solution to perform a reaction, so as to obtain a reaction solution; centrifuging the reaction solution, washing the precipitate, and obtaining ZIF@COD / IR820 nanoparticles; adopting a membrane protein extraction method to obtain Hepa1-6 liver cancer cell membrane fragments and red blood cell membrane fragments; mixing the Hepa1-6 liver cancer cell membrane fragments, the red blood cell membrane fragments and the ZIF@COD / IR820 nanoparticles, and then placing the mixture in a liposome extruder to perform repeated extrusion for several times, so as to obtain HM / ZIF@COD / IR820. The nano reactor prepared by the method can intensify cholesterol consumption of tumor cells, promote tumor cell apoptosis, has excellent homologous targeting, and is beneficial to improving the treatment effect of liver cancer.
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